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6 results on '"Wu, Yuzhuang"'

1. MiR-30a-5p suppresses cell growth and enhances apoptosis of hepatocellular carcinoma cells via targeting AEG-1

Author

He, Rongquan, Yang, Lihua, Lin, Xiaomiao, Chen, Xin, Lin, Xinggu, Wei, Fanglin, Liang, Xiaona, Luo, Yihuan, Wu, Yuzhuang, Gan, Tingqing, Dang, Yiwu, and Chen, Gang

Subjects
Caspase 7, Binding Sites, Carcinoma, Hepatocellular, Time Factors, Caspase 3, Cell Survival, Liver Neoplasms, Membrane Proteins, RNA-Binding Proteins, Apoptosis, Hep G2 Cells, Transfection, Gene Expression Regulation, Neoplastic, MicroRNAs, Genes, Reporter, Humans, Original Article, RNA Interference, Erratum, 3' Untranslated Regions, Cell Adhesion Molecules, Cell Proliferation, Signal Transduction
Abstract

Background: MiR-30a-5p has been reported to play vital roles in the carcinogenesis and progression of various malignancies via different molecular mechanisms. However, the role and target genes of miR-30a-5p in hepatocellular carcinoma (HCC) remain still unclear. In silico analysis finds that there are complementary sequences between the 3’-untrasnlated region of astrocyte elevated gene 1 (AEG-1) and miR-30a-5p. Herein, we investigated the biological function of miR-30a-5p, as well as the potential molecular mechanism via targeting AEG-1 in HCC cells. Materials and methods: MiR-30a-5p inhibitor, miR-30a-5p mimic, AEG-1 siRNAs, as well as their negative controls were transfected into HCC cell lines HepG2, SMMC-7221, HepB3 and SNU449. Then, the in vitro influence and mechanism of miR-30a-5p on cell viability, proliferation, caspase-3/7 activity and apoptosis were studied, as assessed by different methods, including spectrophotometry, fluorimetry, fluorescence microscopy of Hoechst 33342/propidium iodide double chromatin staining, western blot and dual luciferase reporter assay, respectively. Results: MiR-30a-5p mimic markedly inhibited cell growth, also induced caspase-3/7 activity and apoptosis in all four HCC cell lines tested. The strongest effect was observed in HepG2 and SMMC-7721 cells. However, this effect was slightly weaker than that of AEG-1 siRNAs. Transfection of miR-30a-5p mimic led to a markedly reduced AEG-1 protein level and further dual luciferase reporter assay confirmed that AEG-1 was one of the target genes of miR-30a-5p in HCC cells. Conclusions: MiR-30a-5p may play an essential role in the cell growth and apoptosis of HCC cells, partially via targeting AEG-1.

Published
2020

2. Clinicopathological role of miR-30a-5p in hepatocellular carcinoma tissues and prediction of its function with bioinformatics analysis

Author

Huang,Wenting, Chen,Zu-xuan, He,Rongquan, Wu,Yuzhuang, Yin,Shuya, Liang,Xiao-na, Chen,Gang, Yang,Hong, Peng,Zhi-gang, Yang,Lihua, Huang,Wenting, Chen,Zu-xuan, He,Rongquan, Wu,Yuzhuang, Yin,Shuya, Liang,Xiao-na, Chen,Gang, Yang,Hong, Peng,Zhi-gang, and Yang,Lihua

Abstract

Wen-Ting Huang,1,* Zu-xuan Chen,2,* Rong-quan He,2 Yu-zhuang Wu,1 Shu-ya Yin,1 Xiao-na Liang,1 Gang Chen,1 Hong Yang,3 Zhi-gang Peng,2,* Li-hua Yang2,* 1Department of Pathology, 2Department of Medical Oncology, 3Department of Ultrasonography, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, People’s Republic of China *These authors contributed equally to this work Background: It has been reported that deregulation or dysfunction of microRNAs (miRNAs) plays an essential part in the hepatocarcinogenesis. However, the contribution and mechanism of microRNA-30a-5p (miR-30a-5p) in hepatocellular carcinoma (HCC) remains largely unknown. Therefore, our aim was to investigate the clinicopathological role of miR-30a-5p in HCC tissues and explore its potential pathways in this study.Methods: The expression of miR-30a-5p was measured in 95 HCC and adjacent noncancer tissues by real-time reverse transcription quantitative polymerase chain reaction. The relationship between miR-30a-5p expression levels and clinicopathological parameters was also analyzed. Furthermore, the potential target genes of miR-30a-5p were collected via online prediction and literature searching. Gene ontology and pathway enrichment analyses were used to identify the possible function of miR-30a-5p in HCC.Results: Compared with adjacent noncancer tissues (2.23±0.77), expression level of miR-30a-5p was significantly lower in HCC tissues (1.26±0.66, P<0.001). MiR-30a-5p expression was evidently correlated with tumor nodes, metastasis, tumor–node–metastasis stage, portal vein tumor embolus, vascular invasion, and status of tumor capsule (all P<0.05). A total of 878 genes were finally used for the biological informatics analyses. These prospective target genes were highly enriched in various key pathways, for instance, Ubiquitin-mediated proteolysis, Axon guidance, Ne

Published
2016

6. MiR-30a-5p suppresses cell growth and enhances apoptosis of hepatocellular carcinoma cells via targeting AEG-1.

Author

He R, Yang L, Lin X, Chen X, Lin X, Wei F, Liang X, Luo Y, Wu Y, Gan T, Dang Y, and Chen G

Subjects
3' Untranslated Regions, Binding Sites, Carcinoma, Hepatocellular genetics, Carcinoma, Hepatocellular pathology, Caspase 3 metabolism, Caspase 7 metabolism, Cell Adhesion Molecules genetics, Cell Survival, Gene Expression Regulation, Neoplastic, Genes, Reporter, Hep G2 Cells, Humans, Liver Neoplasms genetics, Liver Neoplasms pathology, Membrane Proteins, MicroRNAs genetics, RNA Interference, RNA-Binding Proteins, Signal Transduction, Time Factors, Transfection, Apoptosis, Carcinoma, Hepatocellular metabolism, Cell Adhesion Molecules metabolism, Cell Proliferation, Liver Neoplasms metabolism, MicroRNAs metabolism
Abstract

Background: MiR-30a-5p has been reported to play vital roles in the carcinogenesis and progression of various malignancies via different molecular mechanisms. However, the role and target genes of miR-30a-5p in hepatocellular carcinoma (HCC) remain still unclear. In silico analysis finds that there are complementary sequences between the 3'-untrasnlated region of astrocyte elevated gene 1 (AEG-1) and miR-30a-5p. Herein, we investigated the biological function of miR-30a-5p, as well as the potential molecular mechanism via targeting AEG-1 in HCC cells., Materials and Methods: MiR-30a-5p inhibitor, miR-30a-5p mimic, AEG-1 siRNAs, as well as their negative controls were transfected into HCC cell lines HepG2, SMMC-7221, HepB3 and SNU449. Then, the in vitro influence and mechanism of miR-30a-5p on cell viability, proliferation, caspase-3/7 activity and apoptosis were studied, as assessed by different methods, including spectrophotometry, fluorimetry, fluorescence microscopy of Hoechst 33342/propidium iodide double chromatin staining, western blot and dual luciferase reporter assay, respectively., Results: MiR-30a-5p mimic markedly inhibited cell growth, also induced caspase-3/7 activity and apoptosis in all four HCC cell lines tested. The strongest effect was observed in HepG2 and SMMC-7721 cells. However, this effect was slightly weaker than that of AEG-1 siRNAs. Transfection of miR-30a-5p mimic led to a markedly reduced AEG-1 protein level and further dual luciferase reporter assay confirmed that AEG-1 was one of the target genes of miR-30a-5p in HCC cells., Conclusions: MiR-30a-5p may play an essential role in the cell growth and apoptosis of HCC cells, partially via targeting AEG-1.

Published
2015

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