20 results on '"Wu, Yuan-Zhou"'
Search Results
2. Degradation Behavior of Concrete Under the Compound Effects of Acid-Salt Mist and Load
- Author
-
Wu, Yuan-Zhou, primary, ZHANG, Jie, additional, TANG, Peng, additional, LV, Henglin, additional, HUANG, Tianhua, additional, and Wang, Hui, additional
- Published
- 2023
- Full Text
- View/download PDF
3. Tumor suppressive lncRNA MEG3 binds to EZH2 and enhances CXCL3 methylation in gallbladder cancer
- Author
-
De-Qiang, Li, Yi-Ren, Ding, Jin-Hui, Che, Zhan, Su, Wei-Zhong, Yang, Lu, Xu, Yun-Jiu, Li, Hai-Hong, Wang, and Wu-Yuan, Zhou
- Subjects
Cancer Research ,Apoptosis ,DNA Methylation ,Methylation ,Mice ,Oncology ,Cell Line, Tumor ,Animals ,Heterografts ,Humans ,Enhancer of Zeste Homolog 2 Protein ,Gallbladder Neoplasms ,RNA, Long Noncoding ,Promoter Regions, Genetic ,Chemokines, CXC ,Cell Proliferation - Abstract
Gallbladder cancer is a malignant tumor with a high mortality rate. Accumulating evidence supports that lncRNA MEG3 may halt the progression of gallbladder cancer, while the downstream mechanism is rarely studied. Thus, we aim to investigate the molecular basis of the tumor-suppressing role of lncRNA MEG3 in gallbladder cancer. The expression of lncRNA MEG3 and CXCL3 was measured in patient serum and cell lines of gallbladder cancer. The viability, apoptosis, migration, and invasion of gallbladder cancer cells were assessed following ectopic MEG3 expression, as detected by CCK-8, flow cytometry, and Transwell assays. The interaction among lncRNA MEG3, EZH2, and CXCL3 was explored through ChIP, RNA pull-down, and RIP assays. The effects of lncRNA MEG3 and CXCL3 on tumor growth were evaluated by a mouse xenograft model. lncRNA MEG3 was expressed at a low level in gallbladder cancer patient serum and cell lines, while CXCL3 was highly expressed. MEG3 overexpression repressed the malignant behaviors of gallbladder cancer cells and promoted their apoptosis. MEG3 was mainly localized in the nucleus. MEG3 bound to EZH2, and EZH2 catalyzed the H3K27 trimethylation of the CXCL3 promoter region. MEG3 downregulated CXCL3 by activating EZH2-mediated H3K27 trimethylation of CXCL3; MEG3 overexpression attenuated cancer cell malignant behaviors in vitro and suppressed tumor growth in vivo in gallbladder cancer by inhibiting CXCL3 expression. Altogether, our results indicate that lncRNA MEG3 impedes gallbladder cancer development via the EZH2-CXCL3 axis, offering potential biomarkers for gallbladder cancer management.
- Published
- 2022
4. Degradation model of bond performance between deteriorated concrete and corroded deformed steel bars
- Author
-
Wu, Yuan-Zhou, Lv, Heng-Lin, Zhou, Shu-Chun, and Fang, Zhong-Nian
- Subjects
Concrete -- Analysis -- Mechanical properties ,Steel -- Corrosion ,Steel bars -- Analysis -- Mechanical properties ,Business ,Construction and materials industries - Abstract
ABSTRACT An experimental investigation was conducted to explore the degradation model of bond stress between concrete and deformed steel bars with four different diameters (12, 16, 20 and 25 mm) [...]
- Published
- 2016
- Full Text
- View/download PDF
5. Hybrid Bayesian-Bp Neural Network-Based Strength Degradation Model of Corroded Reinforcements Under the Colliery Environment
- Author
-
Zheng, Xiao-Wei, primary, Zhou, Shu-Chun, additional, Lv, Heng-Lin, additional, Wu, Yuan-Zhou, additional, and Wang, Hui, additional
- Published
- 2022
- Full Text
- View/download PDF
6. Deterioration behavior of reinforced concrete beam under compound effects of acid-salt mist and carbon dioxide
- Author
-
Lv, Heng-Lin, Wu, Yuan-Zhou, Fang, Zhong-Nian, and Zhou, Shu-chun
- Subjects
Carbon dioxide -- Analysis -- Properties ,Corrosion and anti-corrosives -- Analysis ,Business ,Construction and materials industries - Abstract
ABSTRACT An experimental investigation on the deterioration behavior of reinforced concrete (RC) beams under compound effects of acid-salt mist and carbon dioxide was conducted. The changes of failure mode, neutralization [...]
- Published
- 2015
- Full Text
- View/download PDF
7. Degradation Behaviour of Deteriorated RC Beams Strengthened with CFRP under the Compound Effects of Acid-Salt Mist and Carbon Dioxide
- Author
-
Wang, Wei, primary, Wu, Yuan-zhou, additional, and Fang, Zhongnian, additional
- Published
- 2021
- Full Text
- View/download PDF
8. CXCL12/CXCR4 axis plays pivotal roles in the organ-specific metastasis of pancreatic adenocarcinoma: A clinical study
- Author
-
De-xian Zhang, Sheng Li, Weixia Zhong, Jian-Bo Zhang, Yu-Hui Li, Wu-Yuan Zhou, Yongsheng Gao, Jujie Sun, and Weiwei Chen
- Subjects
Cancer Research ,Pathology ,medicine.medical_specialty ,chemokines ,chemokine receptors ,Metastasis ,Immunology and Microbiology (miscellaneous) ,Pancreatic cancer ,medicine ,organ-specific ,business.industry ,Cancer ,pancreatic neoplasms ,General Medicine ,Articles ,medicine.disease ,biological factors ,medicine.anatomical_structure ,embryonic structures ,Adenocarcinoma ,Immunohistochemistry ,CA19-9 ,Lymph ,biological phenomena, cell phenomena, and immunity ,business ,Pancreas - Abstract
Pancreatic cancer is one of the most lethal types of cancer, and curative resection is only applicable to potentially limited cases due to early metastasis and local invasion. This study reports the influence of CXCL12 and its receptor CXCR4 on the progression of pancreatic cancer and highlights the correlation between the CXCL12/CXCR4 axis and the organ-specific metastasis of pancreatic adenocarcinoma (PAC). A total of 34 patients with pancreatic cancer participated in the current study. The expression of CXCL12 and CXCR4 in cancerous tissues, paracancerous tissues, normal pancreas and lymph nodes surrounding the pancreas were investigated using immunohistochemistry and RT-PCR; furthermore, their relationship with clinicopathological factors was explored (PV9000 method). The positive rate of CXCL12 protein was 13.3% (4/30), the positive rate of CXCR4 protein was 80% (24/30) in tumor tissues. Additionally, a significant correlation between the expression pattern of the CXCL12/CXCR4 axis with lymph node metastasis was identified (P0.05). Also, the positive rate of CXCL12 protein was 50% (15/30), the positive rate of CXCR4 protein was 73.3% (22/30) in the lymphocytes in lymph nodes surrounding the pancreas. Furthermore, we found that CXCL12 and CXCR4 expression in paratumorous vessels and neural tissue were significantly strongly positive. The paratumorous vessels and neural tissue with positive CXCL12 and CXCR4 expression were invaded by CXCL12-positive pancreatic cancer cells. The chemotactic interaction between CXCR4 and its ligand CXCL12 may be a critical event during the progression of pancreatic cancer. The CXCL12/CXCR4 axis plays an important role in the progression and organ-specific metastasis of pancreatic adenocarcinoma.
- Published
- 2012
9. The chemotactic interaction between CCL21 and its receptor, CCR7, facilitates the progression of pancreatic cancer via induction of angiogenesis and lymphangiogenesis
- Author
-
Bo Zhang, Sheng Li, Kai Cui, Chang-Liang Wang, Wen-Hua Zhao, Bin Zhao, Ai-Liang Wang, and Wu-Yuan Zhou
- Subjects
Adult ,Male ,Receptors, CCR7 ,endocrine system ,Angiogenesis ,Blotting, Western ,C-C chemokine receptor type 7 ,Real-Time Polymerase Chain Reaction ,Surgical oncology ,Cell Line, Tumor ,Pancreatic cancer ,medicine ,Humans ,RNA, Neoplasm ,Lymphangiogenesis ,Aged ,Retrospective Studies ,Chemokine CCL21 ,Neovascularization, Pathologic ,Hepatology ,business.industry ,Middle Aged ,medicine.disease ,Immunohistochemistry ,Pancreatic Neoplasms ,Real-time polymerase chain reaction ,Immunology ,Disease Progression ,Cancer research ,Adenocarcinoma ,Female ,Surgery ,business - Abstract
In this study, we report the influence of CCL21 and its receptor, CCR7, on the progression of pancreatic cancer and illuminates the correlation between the CCL21/CCR7 axis and the angiogenesis and lymphangiogenesis of pancreatic adenocarcinoma (PAC). A total of 30 patients with pancreatic cancer was involved in the current study. The expression of CCL21 and CCR7 in cancerous tissues, paracancerous tissues and normal pancreas were investigated using real-time PCR, Western blot and immunohistochemistry, respectively. In addition, we assessed microvessel density (MVD) and microlymphatic vessel density (MLVD) in tumor tissues using immunohistochemistry. Compared to paracancerous tissues and normal pancreas, CCL21 expression in cancerous tissues was detected at a significantly low level. In contrast, the CCR7 expression was considerably higher in cancerous tissues than in normal pancreas and paracancerous tissues. Additionally, a significant correlation between the expression pattern of the CCL21/CCR7 axis and clinicopathological features, such as lymph node metastasis, was identified. Furthermore, we found that CCL21 expression was significantly associated with MVD but not significantly associated with MLVD, while CCR7 expression was significantly associated with MLVD but not significantly associated with MVD. The chemotactic interaction between CCR7 and its ligand, CCL21, may be a critical event during progression in pancreatic cancer, and its underlying mechanism may be induction of angiogenesis and lymphangiogenesis regulated by this chemotactic interaction.
- Published
- 2011
10. Expression of vascular endothelial growth factors-C and -D correlate with evidence of lymphangiogenesis and angiogenesis in pancreatic adenocarcinoma
- Author
-
Wu-Yuan Zhou, Bo Zhang, Wen-Sheng Yu, Jinming Yu, Wen-Hua Zhao, and Sheng Li
- Subjects
Adult ,Male ,Cancer Research ,Pathology ,medicine.medical_specialty ,Angiogenesis ,Vascular Endothelial Growth Factor C ,Vascular Endothelial Growth Factor D ,Adenocarcinoma ,chemistry.chemical_compound ,medicine ,Humans ,Lymphangiogenesis ,Aged ,Neovascularization, Pathologic ,business.industry ,Middle Aged ,medicine.disease ,Immunohistochemistry ,Pancreatic Neoplasms ,Vascular endothelial growth factor ,Oncology ,Vascular endothelial growth factor C ,chemistry ,Tumor progression ,Disease Progression ,Female ,business - Abstract
Background : We analyzed the intratumoral and peritumoral microvessel density (MVD) and microlymphatic vessel density (MLVD) in pancreatic adenocarcinoma (PAC) and recorded the expression of vascular endothelial growth factor (VEGF)-C and -D. These data were tested for their significance for tumor progression. Methods : The tissue samples were obtained from 30 patients with PAC. The expression of VEGF-C and -D, MLVD, MVD was assayed by immunohistochemical staining. The expression of VEGF-A and -C, and -D mRNA was detected by semi-quantitative RT-PCR. Results : Immunohistochemical analysis revealed the presence of VEGF-C and -D immunoreactivity in 73% (22/30) and 57% (17/30). The positive rates of VEGF-C and -D protein in central portion of tumors (30% and 16.7%) were significantly lower than those in marginal portion (73.3% and 56.7%). The group with high expression of VEGF-C and -D in marginal portion had higher incidence of lymph node metastasis, lymphatic invasion and venous invasion. The MLVD in both of the VEGF-C and -D positive groups was higher than that in the negative groups, and the lymph node metastasis increased. MVD in the VEGF-C positive group was higher than that in the negative group. Conclusions : The expression of VEGF-C and -D in the marginal portion of tumor significantly associated with lymphatic metastasis and prognosis in patients with PAC, and may induced lymphangiogenesis. VEGF-C was important in the regulation of angiogenesis and lymphangiogenesis in PAC.
- Published
- 2007
11. Flexural Behavior of RC Beams Under Combined Effects of Acid–Salt Mist and Carbon Dioxide
- Author
-
Wu Yuan-Zhou, Fang Zhong-Nian, and Lv Heng-Lin
- Subjects
Materials science ,flexural capacity ,Structural Engineering ,Carbonation ,Mist ,Rebar ,coupling effects of multi-factors ,Compression (physics) ,colliery ground environment ,Civil Engineering ,Corrosion ,law.invention ,Flexural strength ,law ,Coupling (piping) ,Composite material ,deterioration ,RC beams ,Beam (structure) - Abstract
The coupling effects of hydrochloric acid mist, carbon dioxide, and salt mist rich in Cl– and SO4 2– on the degradation of reinforcement concrete (RC) beams were researched with the simulation of colliery ground environment (CGE) and experimental investigation. The results indicated that carbonation of concrete and corrosion of rebar increased slowly as the maximum width of crack became
- Published
- 2014
12. Mechanisms of TRAIL and gemcitabine induction of pancreatic cancer cell apoptosis
- Author
-
Bin, Zhao, Lu, Li, Kai, Cui, Chang-Liang, Wang, Ai-Liang, Wang, Zi-Qiang, Sun, Bo, Zhang, Wu-Yuan, Zhou, Zuo-Xing, Niu, He, Tian, Yan, Xue, and Sheng, Li
- Subjects
Caspase 3 ,Intracellular Signaling Peptides and Proteins ,Cytochromes c ,Down-Regulation ,Apoptosis ,X-Linked Inhibitor of Apoptosis Protein ,Deoxycytidine ,Gemcitabine ,Up-Regulation ,Mitochondrial Proteins ,Pancreatic Neoplasms ,TNF-Related Apoptosis-Inducing Ligand ,Cell Line, Tumor ,Humans ,Apoptosis Regulatory Proteins ,Cell Proliferation - Abstract
The aim of this study was to investigate induction of apoptosis by the tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) and gemcitabine in the pancreatic cancer cell line SW1990. The sensitivity of SW1990 cells to TRAIL and/or gemcitabine-induced apoptosis and the rate of apoptosis were assessed by MTT assay and flow cytometry, respectively. We used Hoechst 33342 staining to observe apoptotic morphology and expression levels of proteins were analyzed by Western blottin. Growth inhibition and apoptosis rates on treatment with the combination of TRAIL and gemcitabine were significantly higher than with each drug alone (p0.05). Pancreatic cancer cells exhibited a typical apoptosis morphology after treatment with TRAIL or gemcitabine. The levels of cellular apoptosis-associated proteins such as Smac/DIABLO, Cyto C, and the activated fragment of caspase-3 (P17) increased, but the expression of XIAP was significantly decreased after 24 h (p0.05). SW1990 cells responded to TRAIL and/or gemcitabine-induction of apoptosis in a time and concentration-dependent manner. The mechanism of the apoptosis-sensitization effect appeared associated with significant up-regulation of Smac/DIABLO and cytochrome C, down-regulation of XIAP, and activation of caspase-3.
- Published
- 2012
13. [The study of the relationship between microlymphatic vessel density and lymph metastasis in hepatocellular carcinoma]
- Author
-
Xi-Kun, Zhang, Wu-Yuan, Zhou, Zeng-Jun, Li, Ju-Jie, Sun, Wei-Xia, Zhong, and Xue-Tao, Shi
- Abstract
Not Abstract.
- Published
- 2011
14. The CXCR4-CXCL12 pathway facilitates the progression of pancreatic cancer via induction of angiogenesis and lymphangiogenesis
- Author
-
Bo Zhang, Wen-Hua Zhao, Jinming Yu, Kai Cui, Chang-Liang Wang, Sheng Li, Ai-Liang Wang, Ziqiang Sun, and Wu-Yuan Zhou
- Subjects
Adult ,Male ,Pathology ,medicine.medical_specialty ,Receptors, CXCR4 ,Angiogenesis ,CXCR4 ,Neovascularization ,Pancreatic cancer ,Medicine ,Humans ,Lymphangiogenesis ,Aged ,Neoplasm Staging ,Neovascularization, Pathologic ,business.industry ,Middle Aged ,medicine.disease ,biological factors ,Chemokine CXCL12 ,Gene Expression Regulation, Neoplastic ,Pancreatic Neoplasms ,medicine.anatomical_structure ,Lymphatic Metastasis ,embryonic structures ,Disease Progression ,Adenocarcinoma ,Immunohistochemistry ,Surgery ,Female ,biological phenomena, cell phenomena, and immunity ,medicine.symptom ,Neoplasm Grading ,business ,Pancreas ,Signal Transduction - Abstract
Background This study reports the influence of CXCL12 and its receptor CXCR4 on the progression of pancreatic cancer and illuminates the correlation between the CXCL12/CXCR4 axis and the angiogenesis and lymphangiogenesis of pancreatic adenocarcinoma (PAC). Methods A total of 30 patients with pancreatic cancer participated in the current study. The expression of CXCL12 and CXCR4 in cancerous tissues, paracancerous tissues, normal pancreas, and lymph nodes surrounding the pancreas were investigated using real-time PCR and immunohistochemistry, respectively. In addition, we assessed microvessel density (MVD) and microlymphatic vessel density (MLVD) in tumor tissues using immunohistochemistry. Results CXCL12 expression in tumor tissues was significantly lower than that of paracancerous tissues, normal pancreas, and lymph nodes. In contrast, CXCR4 expression in cancerous tissues was considerably higher than that of normal pancreas. Additionally, a significant correlation between the expression pattern of the CXCL12/CXCR4 axis and clinicopathologic features, such as lymph node metastasis, was identified. Furthermore, we found that CXCL12 expression was significantly associated with MVD but not significantly associated with MLVD, while CXCR4 expression was significantly associated with MLVD but not significantly associated with MVD. Conclusions The chemotactic interaction between CXCR4 and its ligand CXCL12 may be a critical event during the progression of pancreatic cancer. The underlying mechanism may be the induction of angiogenesis and lymphangiogenesis regulated by the interaction of CXCL12 and CXCR4.
- Published
- 2009
15. [Quantitative analysis and significance of CXCL12 and CXCR4 expression with lymphangiogenesis of pancreatic adenocarcinoma]
- Author
-
Li-ming, Fei, Chang-liang, Wang, Wen-hua, Zhao, Kai, Cui, Bo, Zhang, Wu-yuan, Zhou, Wei-xia, Zhong, and Sheng, Li
- Subjects
Adult ,Male ,Pancreatic Neoplasms ,Receptors, CXCR4 ,Lymphatic Metastasis ,Humans ,Female ,Lymphangiogenesis ,Middle Aged ,Chemokine CXCL12 ,Aged - Abstract
To investigate the expression of CXCL12, its receptor CXCR4 and its correlations with clinical pathology and lymphangiogenesis in pancreatic adenocarcinoma (PAC).The tissue samples were obtained from 30 patients with PAC by surgery between January 2005 and December 2007, which including PAC, the cancerous peripheral tissues, the normal pancreatic tissues and peripheral lymph nodes. The patients age ranged from 35 to 78 years old (median 57.2 years old). The expressions of CXCL12 and CXCR4 in these tissues were assayed by immunohistochemical staining, RT-PCR and fluorescence quantitative real-time PCR.In the immunohistochemical staining, the CXCL12 protein mainly located in the normal pancreatic cell envelopes and/or cytolymphs. In the immunohistochemical staining, the CXCR4 protein mainly located in the cell envelopes and/or cytolymphs of PAC. The results of RT-PCR and fluorescence quantitative real-time PCR indicated that the expression levels of CXCR4 mRNA in PAC tissues, the cancerous peripheral tissues and peripheral lympho nodes were higher than that in the normal pancreatic tissues (P0.01). The MLVD in PAC were detected by morphometric analysis respectively. The level of MLVD in III-IV stages was higher than I-II stages of PAC (P0.01), and in these cases which had lymphatic metastasis, the level of MLVD significantly increased (P0.01). And there was no correlation between the differentiation and histology types of PAC (P0.05). There was 22 samples that the CXCR4 protein was positive, and among these samples the MLVD was higher than that in negative group of CXCR4 protein (P = 0.003).The expression of CXCR4 was significantly associated with lymphatic metastasis of PAC, and the higher expression of CXCR4 in PAC tissues was significantly associated with lymphangiogenesis of PAC.
- Published
- 2009
16. A fatal case of malignant atrophic papulosis
- Author
-
Sheng Li, Ling Li, Wu-Yuan Zhou, Meng Guo, Fang-Lian Jiao, Yan Li, Chun-Ying Wang, Li-Min Zhao, and Wen-Hua Zhao
- Subjects
medicine.medical_specialty ,Traditional medicine ,business.industry ,medicine ,Degos disease ,Dermatology ,medicine.disease ,business ,Malignant Atrophic Papulosis - Abstract
Auteur(s) : Wen-Hua Zhao1, Chun-Ying Wang1, Wu-Yuan Zhou1, Meng Guo1, Yan Li1, Li-Min Zhao1, Fang-Lian Jiao1, Ling Li2, Sheng Li1 1Shandong Tumor Hospital, Jinan 250117, Shandong Province, China 2Shandong Academy of Medicine Sciences, Jinan 250086, Shandong Province, China Malignant atrophic papulosis (MAPs, otherwise known as Degos disease) has a purely cutaneous variant and a systemic variant with cutaneous manifestations. We describe a 41-year-old woman who presented with a widespread skin [...]
- Published
- 2009
17. Effect of 5-LOX/COX-2 common inhibitor DHDMBF30 on pancreatic cancer cell Capan2
- Author
-
Ming Lv, Sheng Li, Chang-Liang Wang, Wu-Yuan Zhou, Bo Zhang, Wei-xia Zhong, Wen-Hua Zhao, Wen-Sheng Yu, Yan Zhang, and Chun-Ying Wang
- Subjects
medicine.medical_specialty ,Cell division ,Mice, Nude ,Apoptosis ,Flow cytometry ,Mice ,Nude mouse ,Pancreatic cancer ,Internal medicine ,medicine ,Animals ,Humans ,MTT assay ,Lipoxygenase Inhibitors ,Furans ,Mice, Inbred BALB C ,Arachidonate 5-Lipoxygenase ,Cyclooxygenase 2 Inhibitors ,medicine.diagnostic_test ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,business.industry ,Gene Amplification ,Gastroenterology ,General Medicine ,Flow Cytometry ,medicine.disease ,biology.organism_classification ,Pancreatic Neoplasms ,Transplantation ,Basic Research ,Endocrinology ,Cyclooxygenase 2 ,Cell culture ,Cancer research ,business ,Cell Division - Abstract
AIM: To study the effect of 5-lipoxygenase/cyclooxy-genase-2 (5-LOX/COX-2) dual inhibitor 7-tert-butyl-2, 3-dihydro-3, 3-dimethyl substituted dihydrofuran 30 (DHDMBF30) on proliferation and apoptosis of the pancreatic cancer cell line Capan-2 and the effect of DHDMBF30 on human pancreatic cancer in a nude mouse model. METHODS: Investigate the effect of 5-LOX/COX-2 dual inhibitor DHDMBF30 on proliferation and apoptosis of the pancreatic cancer cell line Capan-2 by RT-PCR, MTT assay, FCM and electron microscope. Cell line Capan-2 was inoculated percutaneously on the outer thigh of 12 nude mice. The VEGF mRNA of transplantation tumor was detected by RT-PCR. RESULTS: DHDMBF30 inhibits the proliferation of cell line Capan2, reduces the expression of 5-LOX, COX-2 and VEGF. After Capan2 was treated with DHDMBF30, we found that the apoptosis peak of the experimental group was significantly higher than that of the contrast group (3.08 ± 1.89 vs 27.67 ± 0.52, P < 0.001). The tumor weight of the DHDMBF30 group was significantly lower than PBS control groups (1.35 ± 0.47 vs 2.92 ± 0.73, P < 0.01). Expression of VEGF in the DHDMBF30 group was significantly decreased. CONCLUSION: DHDMBF30 inhibits the proliferation of the pancreatic cell line Capan2, and induces apoptosis and inhibits the growth of pancreatic cancer in nude mice.
- Published
- 2008
18. The chemotactic interaction between CCL21 and its receptor, CCR7, facilitates the progression of pancreatic cancer via induction of angiogenesis and lymphangiogenesis.
- Author
-
Bin Zhao, Kai Cui, Chang-Liang Wang, Ai-Liang Wang, Bo Zhang, Wu-Yuan Zhou, Wen-Hua Zhao, and Sheng Li
- Published
- 2011
- Full Text
- View/download PDF
19. [Effects of minimally invasive versus open esophagectomy on circulating tumor cells in patients with esophageal cancer].
- Author
-
Guo X, Wu YZ, Jia LF, Li YL, Yan YS, and Chen QQ
- Subjects
- Humans, Postoperative Complications, Treatment Outcome, Esophageal Neoplasms surgery, Esophagectomy methods, Minimally Invasive Surgical Procedures, Neoplastic Cells, Circulating
- Abstract
Objective: To investigate the effects of minimally invasive esophagectomy (MIE) and open esophagectomy (OE) on the level of circulating tumor cells (CTCs) in patients with esophageal cancer (EC)., Methods: A total of 73 patients with EC undergoing MIE (n=38) or OE (n=35) in our department between October, 2015 and October, 2017 were enrolled, with 10 patients with benign esophagus disease and 10 healthy volunteers as controls. The levels of CTCs in the peripheral blood of the participants were detected using CanPatrol
TM technique and analyzed for their association with the operation methods and perioperative complications., Results: CTCs were detected in 60.3% (44/73) of the EC patients but in none of the control subjects. CTC level after the surgery was significantly higher than that during the surgery, and CTC level during the surgery was significantly higher than that before surgery (P<0.001). The preoperative and intra-operative CTC levels were not significantly different between MIE and OE groups (P>0.05), but the postoperative CTC level was significantly lower in MIE group than in OE group, and postoperative increment of CTC level (from the preoperative level) was significantly lower in MIE group than in OE group (P<0.001). The total incidence of postoperative complications was significantly lower in MIE group than in OE group (28.9% vs 54.3%, P=0.023), and in both groups, CTC levels in patients with complications were significantly higher than those in patients without complications (P=0.001 and P=0.005 in MIE and OE groups, respectively)., Conclusion: MIE may help to reduce the number of peripheral blood CTCs early after the operation, and dynamic monitoring CTCs level assists in evaluation of the prognosis of EC patients. CTC level may serve as an indicator for monitoring the prognosis of EC.- Published
- 2018
20. [Establishment of a rabbit model of small diameter vascular graft replacement].
- Author
-
Zhang JQ, Chen KT, Zhang FW, Li SB, Wu YZ, Feng J, Wang WJ, and Yan YS
- Subjects
- Animals, Coronary Artery Bypass, Femoral Artery, Hemodynamics, Rabbits, Vascular Patency, Aorta, Abdominal surgery, Disease Models, Animal, Vascular Surgical Procedures
- Abstract
Objective: To establish an rabbit model that mimics the hemodynamics of the bypass graft after coronary artery bypass surgery., Methods: Sixteen New Zealand rabbits were randomly divided into two groups for abdominal aortic artery replacement using a 3-cm-long ePTFE graft with an inner diameter 4 mm through an incision at 1/3 from the middle to the lower part of the abdomen (group A) or in the lower abdomen (group B). The general conditions of the rabbits, operative time, number of collateral vessels that needed to be ligated, rate of massive intraoperative bleeding, fluctuation of vascular anastomosis after surgery, patency rate of the graft on day 7 after the operation were compared between the two groups., Results: The two groups of rabbits had similar body weight, diameter of the abdominal aortic artery, intraoperative bleeding rate and occlusion rate of the vascular graft at 7 days after the procedure. The operative time was longer in group A, but the difference was not statistically significant. In group A, the number of the vascular branches that needed to be ligated was smaller and the rate normal femoral artery pulsation was higher than those in group B., Conclusion: It is feasible to establish models of small diameter vascular graft replacement in rabbits, and the patency rate of the graft can be monitored by observation of the general condition and ultrasound examination of the rabbits.
- Published
- 2017
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.