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222 results on '"Wu, Shengchun"'

19. Detection of bacteria carried by ticks from the mountain areas of Guizhou, Southwestern China

Author

Zhan, Lin, primary, Wu, Shengchun, additional, He, Xian Hai, additional, Zhou, Xiao Quan, additional, Meng, Jiao, additional, Zhou, Cao Min, additional, Yang, Bin, additional, Huang, JianSheng, additional, Yang, Guanghong, additional, Jiang, Jia Fu, additional, Xiao, Zheng, additional, Sun, Yi, additional, Wu, Jia Hong, additional, Cao, Wu Chun, additional, and Yu, Fu xun, additional

Published
2023
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42. miR-125a-5p inhibits colorectal cancer cell epithelial–mesenchymal transition, invasion and migration by targeting TAZ

Author

Tang,Lei, Zhou,Li, Wu,Shengchun, Shi,Xiaoming, Jiang,Guangwei, Niu,Shuai, and Ding,Dianzhu

Subjects
OncoTargets and Therapy
Abstract

Lei Tang,1 Li Zhou,2 Shengchun Wu,1 Xiaoming Shi,1 Guangwei Jiang,1 Shuai Niu,1 Dianzhu Ding11Vascular Surgery, Hebei General Hospital, Shijiazhuang, Hebei 050000, People’s Republic of China; 2Department of Pneumology, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, People’s Republic of ChinaBackground:miR-125a-5p regulated biological processes in various types of cancer, including colorectal cancer (CRC). TAZ, a vital transcriptional coactivators of the Hippo pathway, was found to be overexpressed in various cancers.Objectives: This study aims to study the effect of miR-125a-5p on the progression of CRC by regulating TAZ expression.Methods:In this study, miR-125a-5p and TAZ expression in CRC tissue and cell lines were detected by real-time polymerase chain reaction (RT-PCR). Luciferase reporter assay was applied to detect whether TAZ was a target of miR-125a-5p. Cell migration and invasion were detected in vitro by wound-healing assay and cell invasion assay. Western blot was used to detect the expression of epithelial-mesenchymal transition (EMT)-related proteins.Findings: The results revealed downregulation of miR-125a-5p, as well as upregulation of TAZ in CRC tissue and cell lines. TAZ was identified as a direct target of miR-125a-5p, and its expression was negatively regulated by miR-125a-5p in CRC cell lines. The functional studies revealed that overexpression of miR-125a-5p inhibited the migration, invasion and EMT of CRC cells, while upregulation of TAZ reversed the inhibitory effect caused by miR-125a-5p.Conclusion: Our data suggest that miR-125a-5p inhibits CRC cell migration, invasion and EMT by targeting TAZ. These results suggest that miR-125a-5p serves as a potential therapeutic biomarker for CRC patients.Keywords: miR-125a-5p, TAZ, colorectal cancer, epithelial–mesenchymal transition, migration, invasion &nbsp

Published
2019

50. Mycorrhizal Colonization Status of Lowland Rice (Oryza sativa L.) in the Southeastern Region of China

Author

Chen, Xunwen CIVL, Wu, Fuyong, Li, Hui, Chan, Waifung, Wu, Shengchun, Wong, Ming Hung, Chen, Xunwen CIVL, Wu, Fuyong, Li, Hui, Chan, Waifung, Wu, Shengchun, and Wong, Ming Hung

Abstract

The accumulation, distribution, and speciation of contaminants, such as arsenic, in rice can be affected by soil microorganisms such as arbuscular mycorrhizal fungi (AMF). As a potential measure to control contaminant acquisition in rice, the status and performance of AMF in the field need to be investigated. Root samples of rice plants were collected in seven different cities in Guangdong, Jiangxi, Hubei, and Jiangsu Provinces in China in order to investigate the colonization rate of AMF. The total DNA of the roots was extracted, followed by PCR and sequencing, and further confirmed the existence of AMF. The highest colonization rates (19.5 ± 7.2%) were observed in samples from Huizhou City, Guangdong Province. Sequences of ribosomal DNA derived from Pingtan (PT) and Shuikou (SK) in Huizhou shared a similarity of 73 and 86% to Glomus cf. clarum Att894-7 (FM865542) and “uncultured fungus” (EF434122.1), respectively. The moisture tolerance of the AMF from different sources was tested by subjecting to different levels of water content in the soil. Only AMF from PT, SK, and LJ colonized rice under a condition of 100% of the soil water holding capacity (WHC), but not those isolated from upland plants. The AM colonization rate could be governed by the lighting conditions and temperature. AMF isolated in paddy fields has been shown to have more tolerance to moisture than other upland species. Radial oxygen loss (species and stress dependent) could be an essential factor influencing the colonization rate and requires more investigation. © 2016, Springer-Verlag Berlin Heidelberg.

Published
2017

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