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11. Current commercial dPCR platforms: technology and market review

Author

Tan, Li Ling, Loganathan, Nitin, Agarwalla, Sushama, Yang, Chun, Yuan, Weiyong, Zeng, Jasmine, Wu, Ruige, Wang, Wei, Duraiswamy, Suhanya, Tan, Li Ling, Loganathan, Nitin, Agarwalla, Sushama, Yang, Chun, Yuan, Weiyong, Zeng, Jasmine, Wu, Ruige, Wang, Wei, and Duraiswamy, Suhanya

Abstract

Digital polymerase chain reaction (dPCR) technology has provided a new technique for molecular diagnostics, with superior advantages, such as higher sensitivity, precision, and specificity over quantitative real-time PCRs (qPCR). Eight companies have offered commercial dPCR instruments: Fluidigm Corporation, Bio-Rad, RainDance Technologies, Life Technologies, Qiagen, JN MedSys Clarity, Optolane, and Stilla Technologies Naica. This paper discusses the working principle of each offered dPCR device and compares the associated: technical aspects, usability, costs, and current applications of each dPCR device. Lastly, up-and-coming dPCR technologies are also presented, as anticipation of how the dPCR device landscape may likely morph in the next few years. © 2022 Informa UK Limited, trading as Taylor & Francis Group.

Published
2022

12. Current commercial dPCR platforms: technology and market review.

Author

Tan, Li Ling, Loganathan, Nitin, Agarwalla, Sushama, Yang, Chun, Yuan, Weiyong, Zeng, Jasmine, Wu, Ruige, Wang, Wei, and Duraiswamy, Suhanya

Subjects
POLYMERASE chain reaction, MOLECULAR diagnosis
Abstract

Digital polymerase chain reaction (dPCR) technology has provided a new technique for molecular diagnostics, with superior advantages, such as higher sensitivity, precision, and specificity over quantitative real-time PCRs (qPCR). Eight companies have offered commercial dPCR instruments: Fluidigm Corporation, Bio-Rad, RainDance Technologies, Life Technologies, Qiagen, JN MedSys Clarity, Optolane, and Stilla Technologies Naica. This paper discusses the working principle of each offered dPCR device and compares the associated: technical aspects, usability, costs, and current applications of each dPCR device. Lastly, up-and-coming dPCR technologies are also presented, as anticipation of how the dPCR device landscape may likely morph in the next few years. [ABSTRACT FROM AUTHOR]

Published
2023
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21. Microfluidic chip-capillary electrophoresis device for the determination of urinary metabolites and proteins

Author

YS Fung and Wu Ruige

Subjects
Analyte, Chromatography, Chemistry, Clinical Biochemistry, Microfluidics, Electrophoresis, Capillary, Proteins, General Medicine, Urinalysis, Analytical Chemistry, Integrated devices, Systems Integration, Medical Laboratory Technology, Electrophoresis, Capillary electrophoresis, Microfluidic chip, Standard addition, Lab-On-A-Chip Devices, Humans, General Pharmacology, Toxicology and Pharmaceutics
Abstract

Microfluidic chip-CE (MC-CE) devices have caught recent attention for diagnostic applications in urine. This is due to the successes reported in handling real urine samples by integrating microfluidic chips (MC) with analyte enrichment and sample cleanup to CE with high separation efficiency and sensitive analyte detection. Here, we review the determination of urinary metabolites and proteins by MC-CE devices within the past 7 years. The application scope for MC-CE integrated devices was found to exceed the use of either technique alone, showing comparable performance to laser-induced fluorescence detection using less sensitive UV detectors, offering the flexibility to handle difficult urine samples with on-chip dilution and online standard addition and delivering enhanced performance as compared with commercial microfluidic chip electrophoresis chips.

Published
2015

22. Plug-and-play microvalve and micropump for rapid integration with microfluidic chips

Author

Harvard University--MIT Division of Health Sciences and Technology, Mousavi Shaegh, Seyed Ali, Wang, Zhenfeng, Ng, Sum Huan, Wu, Ruige, Nguyen, Huu Tuan, Chan, Leon Cong Zhi, Toh, Alicia Guek Geok, Wang, Zhiping, Harvard University--MIT Division of Health Sciences and Technology, Mousavi Shaegh, Seyed Ali, Wang, Zhenfeng, Ng, Sum Huan, Wu, Ruige, Nguyen, Huu Tuan, Chan, Leon Cong Zhi, Toh, Alicia Guek Geok, and Wang, Zhiping

Abstract

This paper reports design, fabrication, and characterization of an air-actuated microvalve and a micropump made of thermoplastic materials. The bonding process was carried out by thermal fusion process with no particular surface treatment. The developed microvalve was used as a reversible switch for controlling both liquid flow and electrical field. Bonding strength of the fabricated microvalves could withstand liquid and air pressures of up to 600 kPa with no burst failure. The micropump made of three connected microvalves, actuated by compressed air, could generate a liquid flow rate of up to 85 µl/min. The proposed microvalve and micropump can be used as pre-fabricated off-the-shelf microfluidic functional elements for easy and rapid integration with thermoplastic microfluidic circuitries in a plug-and-play arrangement.

Published
2016

30. Multidimensional microchip-capillary electrophoresis device for determination of functional proteins in infant milk formula.

Author

Wu R, Wang Z, and Fung YS

Subjects
Animals, Humans, Infant, Electrophoresis, Capillary methods, Electrophoresis, Microchip methods, Infant Formula chemistry, Proteins chemistry
Abstract

Functional proteins have been found in infant milk formula as supplements, added by an increasing number of manufacturers. Their supplementations are expected to be controlled and monitored. Here, we describe a microchip-integrated CE method for the determination of these low levels of functional proteins in a protein-rich sample matrix. On-chip isoelectric focusing (IEF) is used to separate high-abundance proteins from low-abundance proteins instead of using some complicated time-consuming protein purification process. After that, transient isotachophoresis hyphenated capillary zone electrophoresis (t-ITP-CZE) can preconcentrate, separate, and analyze transferred functional proteins in the embedded capillary under UV detection.

Published
2015
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