Search

Your search keyword '"Wu, Lixian"' showing total 238 results
Start Over Author "Wu, Lixian"
238 results on '"Wu, Lixian"'

17. Matrix metalloproteinase 2 contributes to adult eclosion and immune response in the small hive beetle, Aethina tumida.

Author

Wu, Lixian, Xu, Yajing, Li, Liangbin, Cao, Dainan, Liu, Fang, and Zhao, Hongxia

Subjects
*MATRIX metalloproteinases, *RNA interference, *SMALL interfering RNA, *CHITIN, *IMMUNE response, *BEETLES
Abstract

During the pupal‐adult eclosion process of holometabolous insects, the old cuticle is shed and replaced by a completely different new cuticle that requires tanning and expansion, along with extensive extracellular matrix (ECM) remodeling. In vertebrates, matrix metalloproteinases (MMPs), a class of zinc‐dependent endopeptidases, play key roles in regulating the ECM that surrounds cells. However, little is known about these extracellular proteinases available in insects. The small hive beetle (SHB), Aethina tumida, is a widespread invasive parasite of honey bees. In this study, 6 MMP homologs were identified in the SHB genome. RNA interference experiments showed that all 6 AtMmps are not required for the larval‐pupal transition, only AtMmp2 was essential for pupal‐adult eclosion in SHB. Knockdown of AtMmp2 resulted in eclosion defects and wing expansion failure, as well as mortality within 3 d of adult eclosion. Transcriptomic analysis revealed that knockdown of AtMmp2 significantly increased expression of the Toll and Imd pathways, chitin metabolism, and cross‐linking (such as the pro‐phenoloxidase activating cascade pathway and the tyrosine‐mediated cuticle sclerotization and pigmentation pathway). These data revealed evolutionarily conserved functions of Mmp2 in controlling adult eclosion and wing expansion, also provided a preliminary exploration of the novel function of regulating Toll and Imd pathways, as well as new insights into how MMPs regulate insect development and defense barriers. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

28. Supplementary Methods and Materials, Figure Legend from Dual Inhibition of Bcr-Abl and Hsp90 by C086 Potently Inhibits the Proliferation of Imatinib-Resistant CML Cells

Author

Wu, Lixian, primary, Yu, Jing, primary, Chen, Ruijia, primary, Liu, Yang, primary, Lou, Liguang, primary, Wu, Ying, primary, Huang, Lisen, primary, Fan, Yingjuan, primary, Gao, Pinzhang, primary, Huang, Meijuan, primary, Wu, Yong, primary, Chen, Yuanzhong, primary, and Xu, Jianhua, primary

Published
2023
Full Text
View/download PDF

29. Albumin-encapsulated HSP90-PROTAC BP3 nanoparticles not only retain protein degradation ability but also enhance the antitumour activity of BP3 in vivo

Author

Jiang, Qingna, primary, Hu, Yan, additional, Liu, Quanyu, additional, Tang, Yuanling, additional, Wu, Xinhua, additional, Liu, Jingwen, additional, Tu, Guihui, additional, Li, Ge, additional, Lin, Xiaoqing, additional, Qu, Minghui, additional, Cai, Yajuan, additional, Huang, Xiuwang, additional, Xu, Jianhua, additional, Deng, Yanping, additional, Chen, Zhuo, additional, and Wu, Lixian, additional

Published
2023
Full Text
View/download PDF

37. Discovery of PARP1-PROTAC as novel strategy against PARP inhibitor resistance via promoting ferroptosis in mutant-p53 triple-negative breast cancer

Author

Wu, Lixian, primary, Li, Ge, additional, Lin, Shanshan, additional, Yu, Zelei, additional, Wu, Xinhua, additional, Liu, Jingwen, additional, Tu, Guihui, additional, Liu, Quanyu, additional, Tang, Yuanling, additional, Jiang, Qingna, additional, Xu, Jianhua, additional, and Huang, Qingling, additional

Published
2022
Full Text
View/download PDF

42. Identification and characterization of CYP307A1 as a molecular target for controlling the small hive beetle, Aethina tumida.

Author

Wu, Lixian, Li, Liangbin, Xu, Yajing, Li, Qiang, Liu, Fang, and Zhao, Hongxia

Subjects
REVERSE transcriptase polymerase chain reaction, DRUG target, MOLTING, METAMORPHOSIS, HEMATOXYLIN & eosin staining, TOPICAL drug administration, ECDYSONE
Abstract

Background: The molting hormone 20‐hydroxyecdysone (20E) plays a key role in insect development, metamorphosis, and reproduction. Previous studies have shown that ecdysteroid metabolism is regulated by a series of CYP genes in most of the insect species. However, the roles of these CYP genes in a Coleopteran beetle, Aethina tumida (small hive beetle, SHB) have not yet been explored. Results: In the current study, we identified seven CYP genes (six Halloween genes and one AtCYP18A1 gene) related to 20E metabolism. Reverse transcription quantitative polymerase chain reaction (RT‐qPCR) showed that AtCYP307A1 and AtCYP307B1 were primarily expressed in the embryonic stage and in the cephalothorax of larvae. RNA interference (RNAi) screening revealed that suppression of AtCYP307A1 expression caused a lethal phenotype during the larval–pupal metamorphosis. Furthermore, Hematoxylin and Eosin staining of the integument showed that the RNAi of AtCYP307A1 inhibited the apolysis and degradation of the old cuticle. In addition, silencing of AtCYP307A1 resulted in significant down‐regulation of 20E titers and the expression levels of 20E signaling pathway genes. Finally, the AtCYP307A1 RNAi phenotype was rescued by topical application of 20E. Conclusion: Our studies suggest that AtCYP307A1 involved in 20E synthesis is indispensable during the larval–pupal metamorphosis of beetles, which could serve as a putative insecticide target for pest control. © 2022 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

48. Effect and Mechanism of Mycobacterium tuberculosis Lipoprotein LpqH in NLRP3 Inflammasome Activation in Mouse Ana-1 Macrophage

Author

Wu Lixian, Chen Xiaowen, Liu Liting, Zhai Kaixin, Chen Yue, and Wang Guofu

Subjects
0301 basic medicine, education, behavioral disciplines and activities, General Biochemistry, Genetics and Molecular Biology, Epitope, Flow cytometry, 03 medical and health sciences, 0302 clinical medicine, Protein structure, Western blot, medicine, Secretion, B cell, General Immunology and Microbiology, medicine.diagnostic_test, Chemistry, Inflammasome, General Medicine, Molecular biology, Protein tertiary structure, 030104 developmental biology, medicine.anatomical_structure, Medicine, 030215 immunology, medicine.drug
Abstract

The study is aimed at investigating the role and mechanism of LpqH of Mycobacterium tuberculosis in the activation of NLRP3 inflammasome in mouse Ana-1 macrophages. ExPASy-ProtParam, PHYRE2, ABCpred, and SYFPEITHI were used to predict and analyze the physicochemical properties, protein structure, and B cell/T cell-associated epitopes of LpqH protein. The recombinant LpqH protein was purified, and its immunoreactivity was analyzed with western blot. The LPS-treated mouse Ana-1 macrophages were incubated with purified LpqH protein directly. The expression of NLRP3, ASC, and caspase-1 protein was detected by western blot. The secretion of IL-1β was detected by ELISA, and LDH was detected by a kit. Cell death was detected by flow cytometry. LpqH consisted of 159 amino acids and was a hydrophobic protein with stable properties. Its secondary structure contained 47% random coils, 53% β-sheets, and 3% α-helix. The tertiary structure showed a relatively loose spatial conformation. Additionally, it had 8 B cell epitopes ( score > 0.8 ) and 10 CTL cell epitopes ( score ≥ 20 ). The recombinant LpqH, which had strong immunoreactivity, significantly increased the levels of NLRP3, ASC, and caspase-1 p20 ( P < 0.01 ) and promoted the secretion of IL-1β by the cells ( P < 0.01 ). In addition, high concentration of KCl significantly inhibited the effect of LpqH on mouse Ana-1 macrophages and reduced the expression of NLRP3, ASC, and caspase-1 p20 ( P < 0.01 ). However, there was no significant change in LDH ( P > 0.05 ). Meanwhile, LpqH protein did not cause additional cell death ( P > 0.05 ). LpqH protein has good immunogenicity and can activate the NLRP3 inflammasome through the potassium efflux pathway without causing cell death.

Published
2021

50. Investigation of biological activity of Alpinia platychilusextracts and its use as a natural preservative in fruits

Author

Wu, Lixian, Hu, Xiu, Li, Yongquan, Lao, Qinghua, Zhong, Yucheng, Li, Qiaoguang, and Zhang, Hui

Abstract

This study was conducted to explore Alpinia platyjchilusextract as plant source preservative instead of chemical preservative. Finding a plant-based preservative is one of the methods to solve the harm caused by chemical preservatives to human and the environment. The results showed that the leaves and stems extracts (L), roots and rhizomes extracts (R) of A. platyjchilushad strong antibacterial and antioxidant activities. R showed an average inhibition rate of 52.74% on six fruiting putrefactive bacteria. N-butanol extracts of roots and rhizomes (NER) inhibited DPPH and ABTS by 98.29% and 99.48%, respectively. The contents of total flavone and phenolics in N-butanol extracts of leaf and stem (NEL) were 10.13 ± 0.25 mg g−1and 20.57 ± 0.89 mg g−1, respectively. Among them, ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) showed that NEL and NER contained gluconic acid, protocatechuic acid, niacinamide, naringin and other active ingredients. Then, the edible coating was prepared by mixing the extract with 2 mg mL−1and 10 mg mL−1Arabic gum. The weight loss rate of peach, kiwi and pear after NEL treatment was only 1.33%, and the control effect could reach 87.65%. Overall, the results demonstrated that the extract of A. platychiluseffectively enhanced the storage ability of fruits, highlighting its potential as a natural preservative material.

Published
2023
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results