Search

Bioenergy from energy crops is a source of negative emissions and carbon-neutral fuels in many 1.5/2 ∘C IPCC pathways. This may compete with other land uses. In contrast, ancillary biomass like by-products and waste is not primarily grown for energy and thus without land/food/feed competition. Here, we examine the availability and environmental impacts of ancillary bioenergy from agricultural sources under 190 circular agroecological strategies using the global food-system model SOLm for the year 2050. We find that there is a diverse option space for the future food and energy system to meet both global warming targets (1.5 ∘C) and food system sustainability (medium to highly organic) – a similar range of ancillary bioenergy global potential (55–65 EJ)from very different food systems (50%–75% organic agriculture and various levels of waste and concentrate feeding reduction). We find three trade-offs between food system sustainability and ancillary bioenergy provision. First, there is a clear trade-off between nutrient recycling and negative emissions potential. 1.4–2.6 GTCO2eq of negative emissions supplied through ancillary bioenergy with carbon capture and storage comes at the cost of nutrient deficits and resulting incompatibility with even a medium degree of organic farming. Second, reducing feed from croplands increases the ancillary bioenergy production with low shares of organic agriculture and reduces it for high shares. Third, food waste reduction reduces ancillary bioenergy provision. Hence, the sustainable transformation of the food system towards a less animal-based diet and waste reduction may conflict with a higher ancillary bioenergy provision, especially when the organic share is high as well. The policy implication of our results is that ancillary bioenergy can provide a similar range of future bioenergy as foreseen in IPCC AR6 illustrative pathways (±10% ) without additional land use or compromising food availability. However, higher ancillary bioe, Energy and Industry

Bioenergy is currently a major renewable energy source in Europe but faces an unclear future because of conflicting modelling results and the lack of long-term policy. This paper identifies three challenges and potential opportunities by analysing bioenergy's historical national deployment, current policy support, and possible future roles in Europe. The first challenge is on the supply side. Calculating the supply-consumption dynamics and import dependency of EU bioenergy, we find that the security of bioenergy supply is challenging for liquid biofuels and those countries with the highest per-capita bioenergy consumption in Europe. Second, the definition of “sustainable bioenergy” in modelling studies is sometimes inconsistent with how EU policies label it. Third, on the demand side, there are unique but competing uses for bioenergy without a clear long-term strategy in Europe. We conclude with three opportunities to tackle these challenges for future research. First, utilising the untapped bioenergy potential with low environmental impacts could improve supply security. A clear and harmonised definition of “sustainable bioenergy” could better convey modelling results to policymaking. Finally, understanding where best to use limited sustainable bioenergy supply through sector-coupled energy system models can provide direction for a clearer EU bioenergy strategy towards 2050., Energie and Industrie

Biomass is a growing renewable energy source in Europe and is envisioned to play a role for realising carbon neutrality, predominantly using dedicated energy crops. However, dedicated biomass is controversial for reasons including its competition with food production or its land-use and emissions impacts. Here we examine the potential role of a land-free alternative: ancillary bioenergy (AB) from biomass sources not primarily grown for energy and without land/food/feed competition. We provide the first dataset of 2050 ancillary biomass potential using the agricultural system model SOLm, which encompasses untapped by-/co-products and detailed agricultural residues. Results show that there is a limited future potential for AB in Europe (2394-10 342 PJ, which is 3-6 times lower than other estimates including dedicated biomass). We design and investigate alternative scenarios where this bioenergy resource can be fully utilised, not utilised at all, or utilised optimally by the sector-coupled energy system model Euro-Calliope. We find that fully utilising ancillary biomass can help phase out controversial nuclear or land-intensive dedicated biomass, so might achieve higher societal acceptability. Using all ancillary biomass as a negative-emissions source at stationary bioenergy carbon capture and storage plants in a nuclear-free system provides additional climate benefits. It is also possible to leave the AB potential completely unused, which barely increases total system cost, but would preserve agricultural nutrients. We conclude that there are synergies and trade-offs among possible strategic uses of AB, which can provide guidelines for a more coherent European bioenergy strategy. Although the 2050 potential of AB is limited, our findings suggest that it could fill critical strategic niches for realising carbon-neutrality., Energie and Industrie

Very few developed economies have a full free trade agreement (FTA) with China. This study employs one GTAP model and builds an extended environmental multi-region input–output model to investigate a hypothetical China-UK FTA, concerning embodied industrial emissions of SO2, PM2.5, NOX, and NH3. The economic sectors are also classified based on their embodied pollution intensity and trade advantage index under various FTA scenarios. Results show that the UK’s GDP and welfare and China’s welfare will increase, along with changes in their trade structures. Overall, this FTA brings about larger net impacts on embodied emissions of SO2 than on PM2.5, NOX and NH3, and both countries are net importers of the latter three pollutants. Key sectors such as non-metallic mineral products, chemical products, and agriculture are inclined to become less competitive and less polluting under the FTA. The inclusion of agri-food sectors exhibits slight counteracting effects in general. The findings are of policy importance as they provide insights into how best to target key sectors, seeking a balance between trade development and environmental protection., Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public., Energie and Industrie

The Josephson effect is one of the most studied macroscopic quantum phenomena in condensed matter physics and has been an essential part of the quantum technologies development over the last decades. It is already used in many applications such as magnetometry, metrology, quantum computing, detectors or electronic refrigeration. However, developing devices in which the induced superconductivity can be monitored, both spatially and in its magnitude, remains a serious challenge. In this work, we have used local gates to control confinement, amplitude and density profile of the supercurrent induced in one-dimensional nanoscale constrictions, defined in bilayer graphene-hexagonal boron nitride van der Waals heterostructures. The combination of resistance gate maps, out-of-equilibrium transport, magnetic interferometry measurements, analytical and numerical modelling enables us to explore highly tunable superconducting weak links. Our study opens the path way to design more complex superconducting circuits based on this principle, such as electronic interferometers or transition-edge sensors., QN/Akhmerov Group

The Josephson effect is one of the most studied macroscopic quantum phenomena in condensed matter physics and has been an essential part of the quantum technologies development over the last decades. It is already used in many applications such as magnetometry, metrology, quantum computing, detectors or electronic refrigeration. However, developing devices in which the induced superconductivity can be monitored, both spatially and in its magnitude, remains a serious challenge. In this work, we have used local gates to control confinement, amplitude and density profile of the supercurrent induced in one-dimensional nanoscale constrictions, defined in bilayer graphene-hexagonal boron nitride van der Waals heterostructures. The combination of resistance gate maps, out-of-equilibrium transport, magnetic interferometry measurements, analytical and numerical modelling enables us to explore highly tunable superconducting weak links. Our study opens the path way to design more complex superconducting circuits based on this principle, such as electronic interferometers or transition-edge sensors., QN/Akhmerov Group

The mechanism by which bacteria divide is not well understood. Cell division is mediated by filaments of FtsZ and FtsA (FtsAZ) that recruit septal peptidoglycan-synthesizing enzymes to the division site. To understand how these components coordinate to divide cells, we visualized their movements relative to the dynamics of cell wall synthesis during cytokinesis. We found that the division septum was built at discrete sites that moved around the division plane. FtsAZ filaments treadmilled circumferentially around the division ring and drove the motions of the peptidoglycan-synthesizing enzymes. The FtsZ treadmilling rate controlled both the rate of peptidoglycan synthesis and cell division. Thus, FtsZ treadmilling guides the progressive insertion of new cell wall by building increasingly smaller concentric rings of peptidoglycan to divide the cell., Accepted Author Manuscript, BN/Cees Dekker Lab

The mechanism by which bacteria divide is not well understood. Cell division is mediated by filaments of FtsZ and FtsA (FtsAZ) that recruit septal peptidoglycan-synthesizing enzymes to the division site. To understand how these components coordinate to divide cells, we visualized their movements relative to the dynamics of cell wall synthesis during cytokinesis. We found that the division septum was built at discrete sites that moved around the division plane. FtsAZ filaments treadmilled circumferentially around the division ring and drove the motions of the peptidoglycan-synthesizing enzymes. The FtsZ treadmilling rate controlled both the rate of peptidoglycan synthesis and cell division. Thus, FtsZ treadmilling guides the progressive insertion of new cell wall by building increasingly smaller concentric rings of peptidoglycan to divide the cell., Accepted Author Manuscript, BN/Cees Dekker Lab

Nanofabricated structures and microfluidic technologies are increasingly being used to study bacteria because of their precise spatial and temporal control. They have facilitated studying many long-standing questions regarding growth, chemotaxis and cell-fate switching, and opened up new areas such as probing the effect of boundary geometries on the subcellular structure and social behavior of bacteria. We review the use of nano/microfabricated structures that spatially separate bacteria for quantitative analyses and that provide topological constraints on their growth and chemical communications. These approaches are becoming modular and broadly applicable, and show a strong potential for dissecting the complex life of bacteria at various scales and engineering synthetic microbial societies., Accepted Author Manuscript, BN/Cees Dekker Lab

Cells owe their internal organization to self-organized protein patterns, which originate and adapt to growth and external stimuli via a process that is as complex as it is little understood. Here, we study the emergence, stability, and state transitions of multistable Min protein oscillation patterns in live Escherichia coli bacteria during growth up to defined large dimensions. De novo formation of patterns from homogenous starting conditions is observed and studied both experimentally and in simulations. A new theoretical approach is developed for probing pattern stability under perturbations. Quantitative experiments and simulations show that, once established, Min oscillations tolerate a large degree of intracellular heterogeneity, allowing distinctly different patterns to persist in different cells with the same geometry. Min patterns maintain their axes for hours in experiments, despite imperfections, expansion, and changes in cell shape during continuous cell growth. Transitions between multistable Min patterns are found to be rare events induced by strong intracellular perturbations. The instances of multistability studied here are the combined outcome of boundary growth and strongly nonlinear kinetics, which are characteristic of the reaction–diffusion patterns that pervade biology at many scales., BN/Cees Dekker Lab

Cells owe their internal organization to self-organized protein patterns, which originate and adapt to growth and external stimuli via a process that is as complex as it is little understood. Here, we study the emergence, stability, and state transitions of multistable Min protein oscillation patterns in live Escherichia coli bacteria during growth up to defined large dimensions. De novo formation of patterns from homogenous starting conditions is observed and studied both experimentally and in simulations. A new theoretical approach is developed for probing pattern stability under perturbations. Quantitative experiments and simulations show that, once established, Min oscillations tolerate a large degree of intracellular heterogeneity, allowing distinctly different patterns to persist in different cells with the same geometry. Min patterns maintain their axes for hours in experiments, despite imperfections, expansion, and changes in cell shape during continuous cell growth. Transitions between multistable Min patterns are found to be rare events induced by strong intracellular perturbations. The instances of multistability studied here are the combined outcome of boundary growth and strongly nonlinear kinetics, which are characteristic of the reaction–diffusion patterns that pervade biology at many scales., BN/Cees Dekker Lab

Nanofabricated structures and microfluidic technologies are increasingly being used to study bacteria because of their precise spatial and temporal control. They have facilitated studying many long-standing questions regarding growth, chemotaxis and cell-fate switching, and opened up new areas such as probing the effect of boundary geometries on the subcellular structure and social behavior of bacteria. We review the use of nano/microfabricated structures that spatially separate bacteria for quantitative analyses and that provide topological constraints on their growth and chemical communications. These approaches are becoming modular and broadly applicable, and show a strong potential for dissecting the complex life of bacteria at various scales and engineering synthetic microbial societies., Accepted Author Manuscript, BN/Cees Dekker Lab

One of the most basic features that pervade biology is the existence of boundaries that separate living cells from their outer environments. Molecules responsible for the internal organization of a living cell must adapt to its boundaries, as the cell grows, divides, and changes in shape and size. In this thesis, we aim to understand how intracellular protein networks and chromosomes perceive boundary geometries and in turn organize themselves to guide proper distribution of material during cell growth and division. To manipulate cell boundaries, we use nanofabricate microchambers to guide living bacterial cells to grow into defined shapes such as circles, triangles, squares and rectangles in a large range of sizes. Quantitative studies using this novel ‘cell-sculpting’ technique reveal that Min proteins form oscillation patterns that sense the symmetry axes of the cell shapes and scale their concentration gradient in adaptation to cell sizes, thus ensuring Escherichia coli bacteria to place their division machineries right at the cell center. Chromosomes in E. coli are found to compact and rearrange under the constraints of cell boundaries and they migrate during cell growth through their interactions with cell membrane. These organizational patterns, together with a versatile formation of the FtsZ cytoskeletal filaments, lead to a remarkable ability of E. coli to robustly and accurately divide in diverse shapes., Bionanoscience, Applied Sciences

One of the most basic features that pervade biology is the existence of boundaries that separate living cells from their outer environments. Molecules responsible for the internal organization of a living cell must adapt to its boundaries, as the cell grows, divides, and changes in shape and size. In this thesis, we aim to understand how intracellular protein networks and chromosomes perceive boundary geometries and in turn organize themselves to guide proper distribution of material during cell growth and division. To manipulate cell boundaries, we use nanofabricate microchambers to guide living bacterial cells to grow into defined shapes such as circles, triangles, squares and rectangles in a large range of sizes. Quantitative studies using this novel ‘cell-sculpting’ technique reveal that Min proteins form oscillation patterns that sense the symmetry axes of the cell shapes and scale their concentration gradient in adaptation to cell sizes, thus ensuring Escherichia coli bacteria to place their division machineries right at the cell center. Chromosomes in E. coli are found to compact and rearrange under the constraints of cell boundaries and they migrate during cell growth through their interactions with cell membrane. These organizational patterns, together with a versatile formation of the FtsZ cytoskeletal filaments, lead to a remarkable ability of E. coli to robustly and accurately divide in diverse shapes., Bionanoscience, Applied Sciences

My graduation project located in South Chicago. The site is an abandoned industry area which is totally empty now. My design developed from urban idea to building idea to detail idea. The group strategy is to use urban farming to activate the site. My building is an urban farming theme restaurant., Complex projects, Architecture, Architecture and The Built Environment

My graduation project located in South Chicago. The site is an abandoned industry area which is totally empty now. My design developed from urban idea to building idea to detail idea. The group strategy is to use urban farming to activate the site. My building is an urban farming theme restaurant., Complex projects, Architecture, Architecture and The Built Environment

Cell division in typical rod-shaped bacteria such as Escherichia coli shows a remarkable plasticity in being able to adapt to a variety of irregular cell shapes. Here, we investigate the roles of the Min system and the nucleoid-occlusion factor SlmA in supporting this adaptation. We study “squeezed” E. coli in narrow nanofabricated channels where these bacteria exhibit highly irregular shapes and large volumes. Despite the severely anomalous morphologies we find that most of these bacteria maintain their ability to divide into two equally sized daughters with an accuracy comparable to that of normal rod-shaped cells (about 4%). Deletion of either slmA or minC shows that the molecular systems associated with these genes are largely dispensable for accurate cell division in these irregular cell shapes. Using fluorescence time-lapse microscopy, we determine that the functionality of the Min system is affected by the cell shape, whereas the localization of a nucleoid relative to the cell division proteins (the divisome) remains unperturbed in a broad spectrum of morphologies, consistent with nucleoid occlusion. The observed positioning of the nucleoid relative to the divisome appears not to be affected by the nucleoid-occlusion factor SlmA. The current study underscores the importance of nucleoid occlusion in positioning the divisome and shows that it is robust against shape irregularities., BN/Bionanoscience, Applied Sciences

Cell division in typical rod-shaped bacteria such as Escherichia coli shows a remarkable plasticity in being able to adapt to a variety of irregular cell shapes. Here, we investigate the roles of the Min system and the nucleoid-occlusion factor SlmA in supporting this adaptation. We study “squeezed” E. coli in narrow nanofabricated channels where these bacteria exhibit highly irregular shapes and large volumes. Despite the severely anomalous morphologies we find that most of these bacteria maintain their ability to divide into two equally sized daughters with an accuracy comparable to that of normal rod-shaped cells (about 4%). Deletion of either slmA or minC shows that the molecular systems associated with these genes are largely dispensable for accurate cell division in these irregular cell shapes. Using fluorescence time-lapse microscopy, we determine that the functionality of the Min system is affected by the cell shape, whereas the localization of a nucleoid relative to the cell division proteins (the divisome) remains unperturbed in a broad spectrum of morphologies, consistent with nucleoid occlusion. The observed positioning of the nucleoid relative to the divisome appears not to be affected by the nucleoid-occlusion factor SlmA. The current study underscores the importance of nucleoid occlusion in positioning the divisome and shows that it is robust against shape irregularities., BN/Bionanoscience, Applied Sciences

Kavli Institute of Nanoscience, Applied Sciences

Kavli Institute of Nanoscience, Applied Sciences