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Objective To study the expression of AT1 and AT2 receptors following the focal cerebral ischemia in the rats. Methods The rat model of middle cerebral artery occlusion (MCAO) was established by minimal invasive craniotomy. The twenty-five SD adult male rats were randomly divided into normal control groups (n=5), sham operation groups (n=5),cerebral ischemia groups(including 1 d, 3 d, 1 w groups after MCAO,n=5) . Immunohistochemistry was used to observe the expression of AT1 and AT2 receptors by anti-AT1 and anti-AT2 antibodies. Results There were a large number of AT1 and AT2 receptors-positive cells in the ischemia border(penumbra) zone. These cells respectively reached the peak in 3 d and 1 w post-injury with the largest number,and the optical density of the AT1 and AT2-positive cells increased strikingly, which showed significantly differences compared with the control groups(p<0.05) . Conclusion The adult brain can express AT1 and AT2 receptors after cerebral ischemia as in the developing brain, which may be an important cell source of angiotensin-receptor system involving the pathological processes of ischemia. [ABSTRACT FROM AUTHOR]

PURPOSE: To evaluate the role of digital image correlation method in measuring the initiation and propagation of polymerization shrinkage of light cured composite resin. METHODS: Six freshly extracted complete molars were collected and divided into 2 groups randomly. The proximal surface was sliced to plane and proximal--occlusal cavity were prepared with 2.0 mm x 2.0 mm x 2.0 mm in size, then the tissue surface was treated with etch and bond. The cavity samples in the first group were filled with 3M Z350, and the samples in the second group were filled with material 3M Valux Plus, the proximal surfaces were dealt with random speckle, then fixed to micro tensile framework. As light curing proceeded for 40 seconds, all the speckled images were collected by digital camera in real-time simultaneously. The digital image correlation analysis software was used to deal these images and achieve the displacement and strains in both horizontal and vertical direction on all pixel points, at last the linear shrinkage ratios were counted and the displacement at free surface of the two materials at the 20th second were analyzed with SPSS 12.01 software package for Student's t test. RESULTS: The results showed displacement and strain distribution were accurately achieved and their correlation ratios (R) were bigger than 0.98. The shrinkage represented anisotropy with different direction and unequal value in 40 seconds. The linear shrinkage values of the two materials were 0.439%-0.75%, 0.526%-0.834% in horizontal direction and 0.253% -1.34%, 0.355%-1.51% in vertical direction, respectively. Considering the displacement at the 20th second, shrinkage of Z350 was lower than V. P's both in horizontal and vertical directions (P<0.05). CONCLUSIONS: This study indicates that digital image correlation method can be used to study the polymerization shrinkage of light-cured composites kinematically and effectively, and calculate displacement and strain continually in full fields. Also this method is much closer lo clinic application, and can give more theoretical and experimental evidence for clinic use. [ABSTRACT FROM AUTHOR]

PURPOSE: To evaluate the clinical application of digital photography in special color teeth, and to explore the role and the problems s of digital photos to improve cosmetic effect. METHODS: Digital photography combined visual color- matching was applied in 50 cases with special color teeth in ceramic restorations selected randomly. Then the satisfactory degree of the patients and dentists was evaluated and the value of L*a*b* between prosthesis and its neighbors was collected by self-developed software Dental ImgShop. Chroma (Cab)and hue (hab°) were calculated according to the value of a and b. The data was analyzed with paired t test using SAS6.12 software package, so as chromatic aberration (ΔE). RESULTS: The satisfactory degree of the patients was 98%, and the satisfaction degree of the dentists was 94%. The range of L, a ,b Cab, hab ° of special color teeth was 54.50-92.29, -0.97-8.12,-0.73-20.85,0.85-21.34,29.73-89.20, respectively. There was no significant difference between the prosthesis and their neighbors statistically (P>0.05). The mean value of chromatic aberration (ΔE) was 0.948 (0.136-3.45), and only 3 cases were unacceptable (ΔE≥52). CONCLUSIONS: Digital photography could record and transmit the color information of special color teeth more exactly, and could improve the patients satisfaction and the cosmetic effect in color measuring. Supported by Research Fund of Natural Science for Young Scholars of Minhang Central Hospital. [ABSTRACT FROM AUTHOR]

Cerebral ischemia affects many especially with the ageing population. The ensuing ischemic reactions include oxidative stress, inflammation, and excitotoxicity among others. In the search for effective therapeutic strategies for cerebral ischemia, activated microglia which are the key player in neuroinflammation are now recognized as a potential therapeutic target. Microglia possess both neurotoxic and neuroprotective roles. They are protective by continuously surveilling the microenvironment, phagocytosing dead cells, secreting trophic factors and sculpting the neuronal connections by removing axons and pruning excess synapses. On the other hand, hyperactivated microglia may impair cerebral oxidative metabolism, and produce excessive proinflammatory mediators that may exacerbate the brain damage. In view of this, suppression of microglial activation has been considered a therapeutic strategy to mitigate microglia-based neuroinflammation in cerebral ischemia. However, balancing the neuroprotective and neurotoxic roles of activated microglia remains a challenging issue. Many traditional Chinese herbal agents have been used in clinic for treatment of cerebral ischemia. Here, we provide an overview of five common Chinese herbs targeting specifically microglia-mediated neuroinflammation in cerebral ischemia. It is hoped that a common parallel may be drawn from their beneficial effects especially in the latter pathological conditions for their better and effective use in the future.

Objective: To investigate the risk factors of pinhole infection in the fractured lower limbs after external fixation., Methods: The case-control study was designed. From May 2009 to May 2014, the clinical data of 272 patients with lower limb fracture treated by external fixation device were collected. All the patients were divided into two groups according to post-operative pinhole infection. There were 29 cases in the case group including 23 males and 6 females. The age of patients in case group ranged from 25 to 77 years old,with the average age of (53.41 ± 12.77) years old. There were 243 cases in control group including 217 males and 26 females. The age of patients in the control group ranged from 27 to 78 years old, with the average age of (48.71 ± 11.87) years old. There were nine risk factors observed in our study including age, gender, fixed time by external fixation device, diabetes, time in bed, smoking, operation condition of other parts in the body, infection condition of other parts in the body., Results: The results of univariate analysis showed that there were statistically significant differences among age (χ² = 15.708, P < 0.001), fixed time by external fixation device (χ² = 11.940, P < 0.001), severity of the lower limb fracture (χ² =15.438, P < 0.001), diabetes (χ² = 8.519, P = 0.004) and time in bed (χ² = 7.165, P = 0.007) between case group and control group. The results of Logistic regression analysis showed that the risk factors of pinhole infection after fixed by external fixation device in the lower limb fracture were the advanced age (OR = 8.327, P < 0.001), fixed time by external fixation device (OR = 6.795, P < 0.001), diabetes (OR = 4.965, P = 0.001) and time in bed (OR = 4.864, P = 0.008)., Conclusion: The advanced age, long fixed time, diabetes and long time in bed could increase the risk of pinhole infection after external fixation in the lower limbs with fracture.

Objective: To investigate the effects of tamoxifen (TMX) combined with coenzyme Q10 (CoQ10) on idiopathic oligoasthenospermia., Methods: A total of 183 patients with idiopathic oligoasthenospermia were randomly divided into a TMX + CoQ10 group (n = 63), a TMX group (n = 61) and a CoQ10 group (n = 59). At the end of 3 and 6 months of treatment, semen analyses and hormone tests were performed, and the results were compared with those obtained before the treatment., Results: Compared with the pre-treatment results, the levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH) and testosterone (T) and sperm concentration were significantly elevated in the TMX + CoQ10 and TMX groups (P < 0.05), but showed no significant difference in the CoQ10 group (P > 0.05); sperm motility and morphologically normal sperm were increased significantly in the TMX + CoQ10 and CoQ10 groups (P < 0.05), and slightly in the TMX group but with no statistically significant difference (P > 0.05)., Conclusion: Tamoxifen combined with CoQ10 can significantly improve sperm concentration, motility and morphology in patients with idiopathic oligoasthenospermia.

Objective: To investigate the toxicity of alpha-terthienyl to the larvae of Aedes albopictus, its influencing factors and effect on the larva development., Methods: Under experimental ultraviolet-A (UVA), the number of dead,pupal or eclosive mosquito larvae was determined on the condition of different doses of alpha-terthienyl and different disposal time in the dark; the number of dead larvae was also determined under sunlight on the condition of different doses of alpha-terthienyl and different disposal time to water., Results: The LC50 of alpha-terthienyl to Aedes albopictus larvae was 2.37 microg/L under UVA. The best effect was shown when the larvae were incubated with alpha-terthienyl 3 h in dark. Alpha-terthienyl could significantly inhibit the larva development and the emergence of the pupae. Under strong sunlight, the larvae were quickly killed by high concentration alpha-terthienyl. The 24 hours effect of alpha-terthienyl was better when it was applied at 5 AM than that of at 10 AM and 1 PM., Conclusion: Alpha-terthienyl is an effective, practicable larvicide which prohibits the growth and development of the larvae of Aedes albopictus.

Purpose: To report the method and effects of anatomical reduction by retro-mandibular incision and miniplate rigid fixation for condylar and subcondylar fractures., Methods: Twenty-four condylar neck and subcondylar fractures including 18 unilateral and 6 bilateral in 24 consecutive patients were studied. The fractures were approached by retromandibular incision and miniplate rigid fixation. Postoperative follow up was 3 to 6 months. The treatment results were evaluated including occlusion,joint functions as well as radiographic assessment of condylar changes., Results: The average mouth opening deviation was 6.2mm before operation, which reduced to 1.4mm 3 months after surgical treatment,3 patients had malocclusion,2 had facial nerve damages., Conclusion: Miniplate rigid fixation by retromandibular incision based on anatomical reduction is an effective procedure in treating severely displaced and dislocated neck of condyle and subcondylar fractures.

Objective: To explore possible mechanisms of hepatic fibrosis by investigating the ultrastructural dynamic changes of liver tissue, especially several kinds of cells related to hepatic fibrosis., Methods: Murine schistosomal hepatic fibrosis model was established by infecting mice with Schistosoma japonicum cercariae. Routine transmission electron microscopy was used to observe the liver tissue. H.E. staining was used for examining the pathological changes., Results: H.E. staining showed that the model was established successfully. Ultrastructural observation showed that at the 6th week after infection, the necrosis of hepatocytes around the acute granulomas occurred; the number of sinusoidal endothelial fenestrae and vitamin A droplets in fat-storing cells decreased; large phagosomes and rough endoplasmic reticulum could be seen in the cytoplasm of Kupffer's cells. At the 8th week, steatosis was found in some hepatocytes, some microvilli emerged on a few inter-hepatocytic surfaces and the inter-hepatocytic spaces were enlarged. Large collagen fibrillar bundles filled in the perisinusoidal spaces, and capillarization of hepatic sinusoids was observed. Secretory vesicles filled with collagen fibrils appeared in the cytoplasm of fat-storing cells with large amount of collagenous fiber bundles surround the cells. Rough endoplasmic reticulum increased in Kupffer's cells. At the 10th week, fat-storing cells were activated and transformed into myofibroblasts. At the 12th week, the number of myofibroblasts decreased but that of fibroblasts and fiber cells increased., Conclusion: Activation of fat-storing cells and transformation from fat-storing cells into myofibroblasts are the critical link in the development of hepatic fibrogenesis following schistosome infection. Kupffer's cells, necrotic hepatocytes and sinusoidal endothelial cells may relate to the activation of fat-storing cells. Capillarization of hepatic sinusoids possibly accelerates the development of hepatic fibrosis.