1. INNO-LiPA DNA line probe assay misidentification of M. smegmatis as Mycobacterium fortuitum complex
- Author
-
Rob J. Rentenaar, Nard G Janssen, Theo van den Broek, David J. Hetem, Jakko van Ingen, Wouter Bekers, Ad C. Fluit, Johannes G. Kusters, and Miranda Kamst
- Subjects
Adult ,Male ,Microbiology (medical) ,HSP65 sequencing ,Sequence analysis ,Inno lipa ,Mycobacterium smegmatis ,Mycobacterium Infections, Nontuberculous ,INNO-LiPA ,Microbial Sensitivity Tests ,Microbiology ,chemistry.chemical_compound ,All institutes and research themes of the Radboud University Medical Center ,Lymphadenitis ,Case report ,Animals ,Humans ,Mycobacterium fortuitum complex ,Diagnostic Errors ,Line Probe Assay ,biology ,Mycobacterium fortuitum ,Chemistry ,General Medicine ,biology.organism_classification ,Mycobacterium fortuitum Complex ,Matrix-assisted laser desorption/ionization ,Treatment Outcome ,lnfectious Diseases and Global Health Radboud Institute for Health Sciences [Radboudumc 4] ,Infectious Diseases ,Molecular Diagnostic Techniques ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Pus specimen ,Cattle ,Reagent Kits, Diagnostic ,DNA - Abstract
Seven weeks after being kicked in the face by a cow, a 34-year-old male patient developed a posttraumatic mycobacterial lymphadenitis. A rapidly growing mycobacterial isolate cultured from a surgically drained lymphadenitis pus specimen was identified as Mycobacterium smegmatis by matrix-assisted laser desorption/ionization mass spectrometry and a combination of ITS-, hsp65-, and 16S rRNA-DNA sequence analysis, but as Mycobacterium fortuitum complex using the commercial INNO-LiPA Mycobacteria v2 line probe assay. As it is unclear if the misidentification of this strain is an exception, more research is required.
- Published
- 2019