1. Dynamics of telomeric repeat-containing RNA expression in early embryonic cleavage stages with regards to maternal age
- Author
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Ana Guío-Carrión, Isabel López de Silanes, Maria A. Blasco, Paweł Kordowitzki, KNOW consortium (Poland MSHE), Ministerio de Economía y Competitividad (España), Comunidad de Madrid, Worldwide Cancer Research (WCR), Fundacion Botin (Spain), Comunidad de Madrid (España), Worldwide Cancer Research, and Botín Foundation
- Subjects
Male ,GERM-CELLS ,Aging ,HOMEOSTASIS ,Time Factors ,Telomeric repeat-containing RNAs ,CLEARANCE ,Cleavage Stage, Ovum ,Fertilization in Vitro ,Biology ,Embryo Culture Techniques ,ACTIVATION ,early embryonic development ,Age related female infertility ,telomere length ,medicine ,LENGTH ,Animals ,oocytes ,Blastocyst ,TRANSCRIPTION ,RATES ,EZH2 ,Epigenomics ,Oocyte Donation ,Embryonic cleavage ,Embryogenesis ,Gene Expression Regulation, Developmental ,Telomere Homeostasis ,RNA ,DELAYED CHILDBEARING ,TERRA ,Cell Biology ,Telomere ,Cell biology ,DNA-Binding Proteins ,Mice, Inbred C57BL ,medicine.anatomical_structure ,reproductive aging ,Cattle ,Female ,Maternal Age ,Transcription Factors ,Priority Research Paper - Abstract
Telomeres are transcribed into long non-coding RNAs known as Telomeric Repeat-Containing RNA (TERRA). They have been shown to be essential regulators of telomeres and to act as epigenomic modulators at extra-telomeric sites. However the role of TERRA during early embryonic development has never been investigated. Here, we show that TERRA is expressed in murine and bovine early development following a wave pattern. It starts at 4-cell stage, reaching a maximum at the 16-cell followed by a decline at the morula and blastocyst stages. Moreover, TERRA expression is not affected by increasing oocyte donor age whereas telomere length does. This indicates that TERRA expression is independent of the telomere length in early development. Our findings anticipate an essential role of TERRA in early stages of development and this might be useful in the future for a better understanding of age related female infertility. Dr P. Kordowitzki was supported by KNOW consortium (Poland MS&HE, Decision No. 05-1/KNOW2/2015) and by a special statutory fund of DRI&P IAR&FR PAS in Olsztyn. The support of Sagrario Ortega and co-workers is gratefully acknowledged. Research in the Blasco Lab is funded by the Spanish Ministry of Economy and Competitiveness Projects (SAF2013-45111-R and SAF2015-72455-EXP), the Comunidad de Madrid Project (S2017/BMD-3770), the World Cancer Research (WCR) Project (16-1177), and the Fundacion Botin (Spain). Sí
- Published
- 2020