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153 results on '"Woodhoo, Ashwin"'

1. p63 controls metabolic activation of hepatic stellate cells and fibrosis via an HER2-ACC1 pathway

Author

Fondevila, Marcos F., Novoa, Eva, Gonzalez-Rellan, Maria J., Fernandez, Uxia, Heras, Violeta, Porteiro, Begoña, Parracho, Tamara, Dorta, Valentina, Riobello, Cristina, da Silva Lima, Natalia, Seoane, Samuel, Garcia-Vence, Maria, Chantada-Vazquez, Maria P., Bravo, Susana B., Senra, Ana, Leiva, Magdalena, Marcos, Miguel, Sabio, Guadalupe, Perez-Fernandez, Roman, Dieguez, Carlos, Prevot, Vincent, Schwaninger, Markus, Woodhoo, Ashwin, Martinez-Chantar, Maria L., Schwabe, Robert, Cubero, Francisco J., Varela-Rey, Marta, Crespo, Javier, Iruzubieta, Paula, and Nogueiras, Ruben

Published
2024
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2. Neddylation of phosphoenolpyruvate carboxykinase 1 controls glucose metabolism

Author

Gonzalez-Rellan, María J., Fernández, Uxía, Parracho, Tamara, Novoa, Eva, Fondevila, Marcos F., da Silva Lima, Natalia, Ramos, Lucía, Rodríguez, Amaia, Serrano-Maciá, Marina, Perez-Mejias, Gonzalo, Chantada-Vazquez, Pilar, Riobello, Cristina, Veyrat-Durebex, Christelle, Tovar, Sulay, Coppari, Roberto, Woodhoo, Ashwin, Schwaninger, Markus, Prevot, Vincent, Delgado, Teresa C., Lopez, Miguel, Diaz-Quintana, Antonio, Dieguez, Carlos, Guallar, Diana, Frühbeck, Gema, Diaz-Moreno, Irene, Bravo, Susana B., Martinez-Chantar, Maria L., and Nogueiras, Ruben

Published
2023
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3. Hepatic levels of S-adenosylmethionine regulate the adaptive response to fasting

Author

Capelo-Diz, Alba, Lachiondo-Ortega, Sofía, Fernández-Ramos, David, Cañas-Martín, Jorge, Goikoetxea-Usandizaga, Naroa, Serrano-Maciá, Marina, González-Rellan, Maria J., Mosca, Laura, Blazquez-Vicens, Joan, Tinahones-Ruano, Alberto, Fondevila, Marcos F., Buyan, Mason, Delgado, Teresa C., Gutierrez de Juan, Virginia, Ayuso-García, Paula, Sánchez-Rueda, Alejandro, Velasco-Avilés, Sergio, Fernández-Susavila, Héctor, Riobello-Suárez, Cristina, Dziechciarz, Bartlomiej, Montiel-Duarte, Cristina, Lopitz-Otsoa, Fernando, Bizkarguenaga, Maider, Bilbao-García, Jon, Bernardo-Seisdedos, Ganeko, Senra, Ana, Soriano-Navarro, Mario, Millet, Oscar, Díaz-Lagares, Ángel, Crujeiras, Ana B., Bao-Caamano, Aida, Cabrera, Diana, van Liempd, Sebastiaan, Tamayo-Caro, Miguel, Borzacchiello, Luigi, Gomez-Santos, Beatriz, Buqué, Xabier, Sáenz de Urturi, Diego, González-Romero, Francisco, Simon, Jorge, Rodríguez-Agudo, Rubén, Ruiz, Asier, Matute, Carlos, Beiroa, Daniel, Falcon-Perez, Juan M., Aspichueta, Patricia, Rodríguez-Cuesta, Juan, Porcelli, Marina, Pajares, María A., Ameneiro, Cristina, Fidalgo, Miguel, Aransay, Ana M., Lama-Díaz, Tomas, Blanco, Miguel G., López, Miguel, Villa-Bellosta, Ricardo, Müller, Timo D., Nogueiras, Rubén, Woodhoo, Ashwin, Martínez-Chantar, María Luz, and Varela-Rey, Marta

Published
2023
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4. TOP-229-YI The E2F2 target glycerophosphodiester phosphodiesterase domain containing 3 is involved in MASLD progression to HCC and related dyslipidemias

Author

Apodaka-Biguri, Maider, primary, Muñoz-Llanes, Nerea, additional, Gonzalez-Romero, Francisco, additional, Aurrekoetxea, Igor, additional, Santos, Beatriz Gómez, additional, Delgado, Igotz, additional, Buque, Xabier, additional, Nieva-Zuluaga, Ane, additional, de Gauna, Mikel Ruiz, additional, Fernández-Puertas, Idoia, additional, Gomez-Jauregui, Paul, additional, Sainz-Ramírez, Natalia, additional, Alfaro-Jiménez, Kendall, additional, Castillero, Estibaliz, additional, Congregado, Daniela Mestre, additional, Woodhoo, Ashwin, additional, Varela-Rey, Marta, additional, Lujambio, Amaia, additional, Zubiaga, Ana, additional, and Aspichueta, Patricia, additional

Published
2024
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5. Neddylation orchestrates the complex transcriptional and posttranscriptional program that drives Schwann cell myelination

Author

Ayuso-García, Paula, primary, Sánchez-Rueda, Alejandro, additional, Velasco-Avilés, Sergio, additional, Tamayo-Caro, Miguel, additional, Ferrer-Pinós, Aroa, additional, Huarte-Sebastian, Cecilia, additional, Alvarez, Vanesa, additional, Riobello, Cristina, additional, Jiménez-Vega, Selene, additional, Buendia, Izaskun, additional, Cañas-Martin, Jorge, additional, Fernández-Susavila, Héctor, additional, Aparicio-Rey, Adrián, additional, Esquinas-Román, Eva M., additional, Ponte, Carlos Rodríguez, additional, Guhl, Romane, additional, Laville, Nicolas, additional, Pérez-Andrés, Encarni, additional, Lavín, José L., additional, González-Lopez, Monika, additional, Cámara, Nuria Macías, additional, Aransay, Ana M., additional, Lozano, Juan José, additional, Sutherland, James D., additional, Barrio, Rosa, additional, Martinez-Chantar, María Luz, additional, Azkargorta, Mikel, additional, Elortza, Félix, additional, Soriano-Navarro, Mario, additional, Matute, Carlos, additional, Sánchez-Gómez, María Victoria, additional, Bayón-Cordero, Laura, additional, Pérez-Samartín, Alberto, additional, Bravo, Susana B., additional, Kurz, Thimo, additional, Lama-Díaz, Tomas, additional, Blanco, Miguel G., additional, Haddad, Saif, additional, Record, Christopher J., additional, van Hasselt, Peter M., additional, Reilly, Mary M., additional, Varela-Rey, Marta, additional, and Woodhoo, Ashwin, additional

Published
2024
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6. Trajectory analysis of hepatic stellate cell differentiation reveals metabolic regulation of cell commitment and fibrosis

Author

Martínez García de la Torre, Raquel A., primary, Vallverdú, Julia, additional, Xu, Qing, additional, Ariño, Silvia, additional, Aguilar-Bravo, Beatriz, additional, Ruiz-Blázquez, Paloma, additional, Fernandez-Fernandez, Maria, additional, Navarro-Gascon, Artur, additional, Blasco-Roset, Albert, additional, Sànchez-Fernàndez-de-Landa, Paula, additional, Pera Garcia, Juan, additional, Romero-Moya, Damià, additional, Ayuso Garcia, Paula, additional, Martínez Sánchez, Celia, additional, Zanatto, Laura, additional, Sererols, Laura, additional, Cantallops Vilà, Paula, additional, Antoine, Bénédicte, additional, Azkargorta, Mikel, additional, Lozano, Juan José, additional, Martínez-Chantar, Maria L, additional, Giorgetti, Alessandra, additional, Elortza, Félix, additional, Planavila, Anna, additional, Varela, Marta, additional, Woodhoo, Ashwin, additional, Zorzano, Antonio, additional, Graupera, Isabel, additional, Moles, Anna, additional, Coll, Mar, additional, Affo, Silvia, additional, and Sancho-Bru, Pau, additional

Published
2024
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7. Neddylation orchestrates the complex transcriptional and posttranscriptional program that drives Schwann cell myelination

Author

Metabole ziekten patientenzorg, Child Health, Infection & Immunity, Ayuso-García, Paula, Sánchez-Rueda, Alejandro, Velasco-Avilés, Sergio, Tamayo-Caro, Miguel, Ferrer-Pinós, Aroa, Huarte-Sebastian, Cecilia, Alvarez, Vanesa, Riobello, Cristina, Jiménez-Vega, Selene, Buendia, Izaskun, Cañas-Martin, Jorge, Fernández-Susavila, Héctor, Aparicio-Rey, Adrián, Esquinas-Román, Eva M., Ponte, Carlos Rodríguez, Guhl, Romane, Laville, Nicolas, Pérez-Andrés, Encarni, Lavín, José L., González-Lopez, Monika, Cámara, Nuria Macías, Aransay, Ana M., Lozano, Juan José, Sutherland, James D., Barrio, Rosa, Martinez-Chantar, María Luz, Azkargorta, Mikel, Elortza, Félix, Soriano-Navarro, Mario, Matute, Carlos, Sánchez-Gómez, María Victoria, Bayón-Cordero, Laura, Pérez-Samartín, Alberto, Bravo, Susana B., Kurz, Thimo, Lama-Díaz, Tomas, Blanco, Miguel G., Haddad, Saif, Record, Christopher J., van Hasselt, Peter M., Reilly, Mary M., Varela-Rey, Marta, Woodhoo, Ashwin, Metabole ziekten patientenzorg, Child Health, Infection & Immunity, Ayuso-García, Paula, Sánchez-Rueda, Alejandro, Velasco-Avilés, Sergio, Tamayo-Caro, Miguel, Ferrer-Pinós, Aroa, Huarte-Sebastian, Cecilia, Alvarez, Vanesa, Riobello, Cristina, Jiménez-Vega, Selene, Buendia, Izaskun, Cañas-Martin, Jorge, Fernández-Susavila, Héctor, Aparicio-Rey, Adrián, Esquinas-Román, Eva M., Ponte, Carlos Rodríguez, Guhl, Romane, Laville, Nicolas, Pérez-Andrés, Encarni, Lavín, José L., González-Lopez, Monika, Cámara, Nuria Macías, Aransay, Ana M., Lozano, Juan José, Sutherland, James D., Barrio, Rosa, Martinez-Chantar, María Luz, Azkargorta, Mikel, Elortza, Félix, Soriano-Navarro, Mario, Matute, Carlos, Sánchez-Gómez, María Victoria, Bayón-Cordero, Laura, Pérez-Samartín, Alberto, Bravo, Susana B., Kurz, Thimo, Lama-Díaz, Tomas, Blanco, Miguel G., Haddad, Saif, Record, Christopher J., van Hasselt, Peter M., Reilly, Mary M., Varela-Rey, Marta, and Woodhoo, Ashwin

Published
2024

8. Methionine and S-adenosylmethionine levels are critical regulators of PP2A activity modulating lipophagy during steatosis

Author

Zubiete-Franco, Imanol, García-Rodríguez, Juan Luis, Martínez-Uña, Maite, Martínez-Lopez, Nuria, Woodhoo, Ashwin, Juan, Virginia Gutiérrez-De, Beraza, Naiara, Lage-Medina, Sergio, Andrade, Fernando, Fernandez, Marta Llarena, Aldámiz-Echevarría, Luis, Fernández-Ramos, David, Falcon-Perez, Juan Manuel, Lopitz-Otsoa, Fernando, Fernandez-Tussy, Pablo, Barbier-Torres, Lucía, Luka, Zigmund, Wagner, Conrad, García-Monzón, Carmelo, Lu, Shelly C, Aspichueta, Patricia, Mato, José María, Martínez-Chantar, María Luz, and Varela-Rey, Marta

Subjects
Biomedical and Clinical Sciences, Clinical Sciences, Digestive Diseases, Nutrition, Chronic Liver Disease and Cirrhosis, Complementary and Integrative Health, Liver Disease, Aetiology, 2.1 Biological and endogenous factors, Oral and gastrointestinal, Animals, Autophagy, Cell Culture Techniques, Disease Models, Animal, Fatty Liver, Hepatocytes, Humans, Methionine, Methylation, Mice, Protein Phosphatase 2, S-Adenosylmethionine, SAMe, Liver, Steatosis, Gnmt, Public Health and Health Services, Gastroenterology & Hepatology, Clinical sciences
Abstract

Background & aimsGlycine N-methyltransferase (GNMT) expression is decreased in some patients with severe non-alcoholic fatty liver disease. Gnmt deficiency in mice (Gnmt-KO) results in abnormally elevated serum levels of methionine and its metabolite S-adenosylmethionine (SAMe), and this leads to rapid liver steatosis development. Autophagy plays a critical role in lipid catabolism (lipophagy), and defects in autophagy have been related to liver steatosis development. Since methionine and its metabolite SAMe are well known inactivators of autophagy, we aimed to examine whether high levels of both metabolites could block autophagy-mediated lipid catabolism.MethodsWe examined methionine levels in a cohort of 358 serum samples from steatotic patients. We used hepatocytes cultured with methionine and SAMe, and hepatocytes and livers from Gnmt-KO mice.ResultsWe detected a significant increase in serum methionine levels in steatotic patients. We observed that autophagy and lipophagy were impaired in hepatocytes cultured with high methionine and SAMe, and that Gnmt-KO livers were characterized by an impairment in autophagy functionality, likely caused by defects at the lysosomal level. Elevated levels of methionine and SAMe activated PP2A by methylation, while blocking PP2A activity restored autophagy flux in Gnmt-KO hepatocytes, and in hepatocytes treated with SAMe and methionine. Finally, normalization of methionine and SAMe levels in Gnmt-KO mice using a methionine deficient diet normalized the methylation capacity, PP2A methylation, autophagy, and ameliorated liver steatosis.ConclusionsThese data suggest that elevated levels of methionine and SAMe can inhibit autophagic catabolism of lipids contributing to liver steatosis.

Published
2016

9. O-GlcNAcylated p53 in the liver modulates hepatic glucose production

Author

Gonzalez-Rellan, Maria J., Fondevila, Marcos F., Fernandez, Uxia, Rodríguez, Amaia, Varela-Rey, Marta, Veyrat-Durebex, Christelle, Seoane, Samuel, Bernardo, Ganeko, Lopitz-Otsoa, Fernando, Fernández-Ramos, David, Bilbao, Jon, Iglesias, Cristina, Novoa, Eva, Ameneiro, Cristina, Senra, Ana, Beiroa, Daniel, Cuñarro, Juan, DP Chantada-Vazquez, Maria, Garcia-Vence, Maria, Bravo, Susana B., Da Silva Lima, Natalia, Porteiro, Begoña, Carneiro, Carmen, Vidal, Anxo, Tovar, Sulay, Müller, Timo D., Ferno, Johan, Guallar, Diana, Fidalgo, Miguel, Sabio, Guadalupe, Herzig, Stephan, Yang, Won Ho, Cho, Jin Won, Martinez-Chantar, Maria Luz, Perez-Fernandez, Roman, López, Miguel, Dieguez, Carlos, Mato, Jose M., Millet, Oscar, Coppari, Roberto, Woodhoo, Ashwin, Fruhbeck, Gema, and Nogueiras, Ruben

Published
2021
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10. Signals that control embryonic Schwann cell development and myelination

Author

Woodhoo, Ashwin

Subjects
612.81
Abstract

The generation of mature Schwann cells from their cells of origin, neural crest cells, proceeds through two transitional steps first neural crest cells are specified to form Schwann cell precursors, which then mature into immature Schwann cells. These then generate the myelinating and non-myelinating Schwann cells found in mature nerves. Each of these steps has been well characterised phenotypically in terms of antigenic profile, survival mechanisms and morphological changes. First of all, using adapted survival assays I found that there is a range of factors that supports the survival of crest-derived glial precursor populations that generate satellite cells and Schwann cells but not of crest cells themselves. I found also that satellite cells develop earlier than Schwann cells in a number of characteristics including survival mechanisms and antigenic profile. I then examined the role of the Notch signalling pathway in the Schwann cell lineage using different in vitro and in vivo studies. I found that only Schwann cell precursor maturation is regulated by Notch signalling during early Schwann cell development. Notch signalling is also important in regulating cell division in immature Schwann cells and it acts as a negative regulator of myelination. During normal development, Notch signalling is attenuated, by a mechanism likely to involve the transcription factor Krox-20, to allow myelination to occur. Finally, in collaboration with Professors W.F. Blakemore and R.J. Franklin (University of Cambridge), I made a comparative analysis of the transplant characteristics of Schwann cell precursors and Schwann cells in two animal models of Multiple Sclerosis. We found that Schwann cell precursors outperform Schwann cells in myelination potential, survival and migratory properties in these animal models.

Published
2007

11. Functional and mechanistic role of lncRNA-A1 in cholangiocarcinoma

Author

Tinahones, Alberto, primary, Vicens, Joan Blázquez, additional, Cañas, Jorge, additional, Capelo, Alba, additional, Lavin, JL, additional, Perugorria Montiel, Maria Jesus, additional, de Barrena, Maite García-Fernandez, additional, Fondevila, Marcos Fernandez, additional, Porteiro, Begoña, additional, Lozano, J, additional, Coll, M, additional, Sancho-Bru, Pau, additional, Gonzalez-Sanchez, Ester, additional, Bañales, Jesús, additional, Milkiewicz, Piotr, additional, Milkiewicz, Malgorzata, additional, Bujanda, Luis, additional, Avila, Ma, additional, Aransay, Ana María, additional, Nogueiras, Ruben, additional, Turnes, Juan, additional, Pelacho, Beatriz, additional, Amengual, J, additional, Fabregat, Isabel, additional, Vaquero, Javier, additional, Entrialgo, Rodrigo, additional, Vicent, Silve, additional, Woodhoo, Ashwin, additional, and Varela-Rey, Marta, additional

Published
2023
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12. Functional and mechanistic role of lncRNA-C1 in cholestatic liver diseases

Author

Vicens, Joan Blázquez, primary, Ruano, Alberto Tinahones, additional, Cañas, Jorge, additional, Borzacchiello, Luigi, additional, Capelo, Alba, additional, Fondevila, Marcos Fernandez, additional, Porteiro, Begoña, additional, Bunyan-Woodcraft, Mason, additional, Ramos, David Fernández, additional, Perugorria Montiel, Maria Jesus, additional, Garcia Pagan, Juan Carlos, additional, Banales, Jesus Maria, additional, Milkiewicz, Piotr, additional, Milkiewicz, Małgorzata, additional, Sangro, Bruno, additional, Bujanda, Luis, additional, Avila, Matías A, additional, Aransay, Ana María, additional, Mato, José M., additional, Turnes, Juan, additional, Pelacho, Beatriz, additional, Nogueiras, Ruben, additional, Martínez-Chantar, María Luz, additional, Woodhoo, Ashwin, additional, and Varela-Rey, Marta, additional

Published
2023
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13. Data from E2F1 and E2F2-Mediated Repression of CPT2 Establishes a Lipid-Rich Tumor-Promoting Environment

Author

González-Romero, Francisco, primary, Mestre, Daniela, primary, Aurrekoetxea, Igor, primary, O'Rourke, Colm J., primary, Andersen, Jesper B., primary, Woodhoo, Ashwin, primary, Tamayo-Caro, Miguel, primary, Varela-Rey, Marta, primary, Palomo-Irigoyen, Marta, primary, Gómez-Santos, Beatriz, primary, de Urturi, Diego Sáenz, primary, Núñez-García, Maitane, primary, García-Rodríguez, Juan L., primary, Fernández-Ares, Larraitz, primary, Buqué, Xabier, primary, Iglesias-Ara, Ainhoa, primary, Bernales, Irantzu, primary, De Juan, Virginia Gutierrez, primary, Delgado, Teresa C., primary, Goikoetxea-Usandizaga, Naroa, primary, Lee, Richard, primary, Bhanot, Sanjay, primary, Delgado, Igotz, primary, Perugorria, Maria J., primary, Errazti, Gaizka, primary, Mosteiro, Lorena, primary, Gaztambide, Sonia, primary, Martinez de la Piscina, Idoia, primary, Iruzubieta, Paula, primary, Crespo, Javier, primary, Banales, Jesus M., primary, Martínez-Chantar, Maria L., primary, Castaño, Luis, primary, Zubiaga, Ana M., primary, and Aspichueta, Patricia, primary

Published
2023
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14. Supplementary Information from E2F1 and E2F2-Mediated Repression of CPT2 Establishes a Lipid-Rich Tumor-Promoting Environment

Author

González-Romero, Francisco, primary, Mestre, Daniela, primary, Aurrekoetxea, Igor, primary, O'Rourke, Colm J., primary, Andersen, Jesper B., primary, Woodhoo, Ashwin, primary, Tamayo-Caro, Miguel, primary, Varela-Rey, Marta, primary, Palomo-Irigoyen, Marta, primary, Gómez-Santos, Beatriz, primary, de Urturi, Diego Sáenz, primary, Núñez-García, Maitane, primary, García-Rodríguez, Juan L., primary, Fernández-Ares, Larraitz, primary, Buqué, Xabier, primary, Iglesias-Ara, Ainhoa, primary, Bernales, Irantzu, primary, De Juan, Virginia Gutierrez, primary, Delgado, Teresa C., primary, Goikoetxea-Usandizaga, Naroa, primary, Lee, Richard, primary, Bhanot, Sanjay, primary, Delgado, Igotz, primary, Perugorria, Maria J., primary, Errazti, Gaizka, primary, Mosteiro, Lorena, primary, Gaztambide, Sonia, primary, Martinez de la Piscina, Idoia, primary, Iruzubieta, Paula, primary, Crespo, Javier, primary, Banales, Jesus M., primary, Martínez-Chantar, Maria L., primary, Castaño, Luis, primary, Zubiaga, Ana M., primary, and Aspichueta, Patricia, primary

Published
2023
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15. Supplementary Table 1 from E2F1 and E2F2-Mediated Repression of CPT2 Establishes a Lipid-Rich Tumor-Promoting Environment

Author

González-Romero, Francisco, primary, Mestre, Daniela, primary, Aurrekoetxea, Igor, primary, O'Rourke, Colm J., primary, Andersen, Jesper B., primary, Woodhoo, Ashwin, primary, Tamayo-Caro, Miguel, primary, Varela-Rey, Marta, primary, Palomo-Irigoyen, Marta, primary, Gómez-Santos, Beatriz, primary, de Urturi, Diego Sáenz, primary, Núñez-García, Maitane, primary, García-Rodríguez, Juan L., primary, Fernández-Ares, Larraitz, primary, Buqué, Xabier, primary, Iglesias-Ara, Ainhoa, primary, Bernales, Irantzu, primary, De Juan, Virginia Gutierrez, primary, Delgado, Teresa C., primary, Goikoetxea-Usandizaga, Naroa, primary, Lee, Richard, primary, Bhanot, Sanjay, primary, Delgado, Igotz, primary, Perugorria, Maria J., primary, Errazti, Gaizka, primary, Mosteiro, Lorena, primary, Gaztambide, Sonia, primary, Martinez de la Piscina, Idoia, primary, Iruzubieta, Paula, primary, Crespo, Javier, primary, Banales, Jesus M., primary, Martínez-Chantar, Maria L., primary, Castaño, Luis, primary, Zubiaga, Ana M., primary, and Aspichueta, Patricia, primary

Published
2023
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16. Supplementary Data from E2F1 and E2F2-Mediated Repression of CPT2 Establishes a Lipid-Rich Tumor-Promoting Environment

Author

González-Romero, Francisco, primary, Mestre, Daniela, primary, Aurrekoetxea, Igor, primary, O'Rourke, Colm J., primary, Andersen, Jesper B., primary, Woodhoo, Ashwin, primary, Tamayo-Caro, Miguel, primary, Varela-Rey, Marta, primary, Palomo-Irigoyen, Marta, primary, Gómez-Santos, Beatriz, primary, de Urturi, Diego Sáenz, primary, Núñez-García, Maitane, primary, García-Rodríguez, Juan L., primary, Fernández-Ares, Larraitz, primary, Buqué, Xabier, primary, Iglesias-Ara, Ainhoa, primary, Bernales, Irantzu, primary, De Juan, Virginia Gutierrez, primary, Delgado, Teresa C., primary, Goikoetxea-Usandizaga, Naroa, primary, Lee, Richard, primary, Bhanot, Sanjay, primary, Delgado, Igotz, primary, Perugorria, Maria J., primary, Errazti, Gaizka, primary, Mosteiro, Lorena, primary, Gaztambide, Sonia, primary, Martinez de la Piscina, Idoia, primary, Iruzubieta, Paula, primary, Crespo, Javier, primary, Banales, Jesus M., primary, Martínez-Chantar, Maria L., primary, Castaño, Luis, primary, Zubiaga, Ana M., primary, and Aspichueta, Patricia, primary

Published
2023
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17. Supplementary Table 2 from E2F1 and E2F2-Mediated Repression of CPT2 Establishes a Lipid-Rich Tumor-Promoting Environment

Author

González-Romero, Francisco, primary, Mestre, Daniela, primary, Aurrekoetxea, Igor, primary, O'Rourke, Colm J., primary, Andersen, Jesper B., primary, Woodhoo, Ashwin, primary, Tamayo-Caro, Miguel, primary, Varela-Rey, Marta, primary, Palomo-Irigoyen, Marta, primary, Gómez-Santos, Beatriz, primary, de Urturi, Diego Sáenz, primary, Núñez-García, Maitane, primary, García-Rodríguez, Juan L., primary, Fernández-Ares, Larraitz, primary, Buqué, Xabier, primary, Iglesias-Ara, Ainhoa, primary, Bernales, Irantzu, primary, De Juan, Virginia Gutierrez, primary, Delgado, Teresa C., primary, Goikoetxea-Usandizaga, Naroa, primary, Lee, Richard, primary, Bhanot, Sanjay, primary, Delgado, Igotz, primary, Perugorria, Maria J., primary, Errazti, Gaizka, primary, Mosteiro, Lorena, primary, Gaztambide, Sonia, primary, Martinez de la Piscina, Idoia, primary, Iruzubieta, Paula, primary, Crespo, Javier, primary, Banales, Jesus M., primary, Martínez-Chantar, Maria L., primary, Castaño, Luis, primary, Zubiaga, Ana M., primary, and Aspichueta, Patricia, primary

Published
2023
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18. Supplementary Figures from E2F1 and E2F2-Mediated Repression of CPT2 Establishes a Lipid-Rich Tumor-Promoting Environment

Author

González-Romero, Francisco, primary, Mestre, Daniela, primary, Aurrekoetxea, Igor, primary, O'Rourke, Colm J., primary, Andersen, Jesper B., primary, Woodhoo, Ashwin, primary, Tamayo-Caro, Miguel, primary, Varela-Rey, Marta, primary, Palomo-Irigoyen, Marta, primary, Gómez-Santos, Beatriz, primary, de Urturi, Diego Sáenz, primary, Núñez-García, Maitane, primary, García-Rodríguez, Juan L., primary, Fernández-Ares, Larraitz, primary, Buqué, Xabier, primary, Iglesias-Ara, Ainhoa, primary, Bernales, Irantzu, primary, De Juan, Virginia Gutierrez, primary, Delgado, Teresa C., primary, Goikoetxea-Usandizaga, Naroa, primary, Lee, Richard, primary, Bhanot, Sanjay, primary, Delgado, Igotz, primary, Perugorria, Maria J., primary, Errazti, Gaizka, primary, Mosteiro, Lorena, primary, Gaztambide, Sonia, primary, Martinez de la Piscina, Idoia, primary, Iruzubieta, Paula, primary, Crespo, Javier, primary, Banales, Jesus M., primary, Martínez-Chantar, Maria L., primary, Castaño, Luis, primary, Zubiaga, Ana M., primary, and Aspichueta, Patricia, primary

Published
2023
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19. Supplementary Table 3 from E2F1 and E2F2-Mediated Repression of CPT2 Establishes a Lipid-Rich Tumor-Promoting Environment

Author

González-Romero, Francisco, primary, Mestre, Daniela, primary, Aurrekoetxea, Igor, primary, O'Rourke, Colm J., primary, Andersen, Jesper B., primary, Woodhoo, Ashwin, primary, Tamayo-Caro, Miguel, primary, Varela-Rey, Marta, primary, Palomo-Irigoyen, Marta, primary, Gómez-Santos, Beatriz, primary, de Urturi, Diego Sáenz, primary, Núñez-García, Maitane, primary, García-Rodríguez, Juan L., primary, Fernández-Ares, Larraitz, primary, Buqué, Xabier, primary, Iglesias-Ara, Ainhoa, primary, Bernales, Irantzu, primary, De Juan, Virginia Gutierrez, primary, Delgado, Teresa C., primary, Goikoetxea-Usandizaga, Naroa, primary, Lee, Richard, primary, Bhanot, Sanjay, primary, Delgado, Igotz, primary, Perugorria, Maria J., primary, Errazti, Gaizka, primary, Mosteiro, Lorena, primary, Gaztambide, Sonia, primary, Martinez de la Piscina, Idoia, primary, Iruzubieta, Paula, primary, Crespo, Javier, primary, Banales, Jesus M., primary, Martínez-Chantar, Maria L., primary, Castaño, Luis, primary, Zubiaga, Ana M., primary, and Aspichueta, Patricia, primary

Published
2023
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20. Neddylation of phosphoenolpyruvate carboxykinase 1 controls glucose metabolism

Author

Agencia Estatal de Investigación (España), Ministerio de Ciencia y Tecnología (España), Xunta de Galicia, Fundación BBVA, Fundación Atresmedia, Fundación la Caixa, European Commission, Centro de Investigación Biomédica en Red Enfermedades Hepáticas y Digestivas (España), Instituto de Salud Carlos III, Gonzalez-Rellan, María J, Fernández, Uxía, Parracho, Tamara, Novoa, Eva, Fondevila, Marcos F, da Silva Lima, Natalia, Ramos, Lucía, Rodríguez, Amaia, Serrano-Maciá, Marina, Perez-Mejias, Gonzalo, Chantada-Vazquez, Pilar, Riobello, Cristina, Veyrat-Durebex, Christelle, Tovar, Sulay, Coppari, Roberto, Woodhoo, Ashwin, Schwaninger, Markus, Prevot, Vincent, Delgado, Teresa C, Lopez, Miguel, Diaz-Quintana, Antonio, Dieguez, Carlos, Guallar, Diana, Frühbeck, Gema, Diaz-Moreno, Irene, Bravo, Susana B, Martinez-Chantar, Maria L, Nogueiras, Ruben, Agencia Estatal de Investigación (España), Ministerio de Ciencia y Tecnología (España), Xunta de Galicia, Fundación BBVA, Fundación Atresmedia, Fundación la Caixa, European Commission, Centro de Investigación Biomédica en Red Enfermedades Hepáticas y Digestivas (España), Instituto de Salud Carlos III, Gonzalez-Rellan, María J, Fernández, Uxía, Parracho, Tamara, Novoa, Eva, Fondevila, Marcos F, da Silva Lima, Natalia, Ramos, Lucía, Rodríguez, Amaia, Serrano-Maciá, Marina, Perez-Mejias, Gonzalo, Chantada-Vazquez, Pilar, Riobello, Cristina, Veyrat-Durebex, Christelle, Tovar, Sulay, Coppari, Roberto, Woodhoo, Ashwin, Schwaninger, Markus, Prevot, Vincent, Delgado, Teresa C, Lopez, Miguel, Diaz-Quintana, Antonio, Dieguez, Carlos, Guallar, Diana, Frühbeck, Gema, Diaz-Moreno, Irene, Bravo, Susana B, Martinez-Chantar, Maria L, and Nogueiras, Ruben

Abstract

Neddylation is a post-translational mechanism that adds a ubiquitin-like protein, namely neural precursor cell expressed developmentally downregulated protein 8 (NEDD8). Here, we show that neddylation in mouse liver is modulated by nutrient availability. Inhibition of neddylation in mouse liver reduces gluconeogenic capacity and the hyperglycemic actions of counter-regulatory hormones. Furthermore, people with type 2 diabetes display elevated hepatic neddylation levels. Mechanistically, fasting or caloric restriction of mice leads to neddylation of phosphoenolpyruvate carboxykinase 1 (PCK1) at three lysine residues-K278, K342, and K387. We find that mutating the three PCK1 lysines that are neddylated reduces their gluconeogenic activity rate. Molecular dynamics simulations show that neddylation of PCK1 could re-position two loops surrounding the catalytic center into an open configuration, rendering the catalytic center more accessible. Our study reveals that neddylation of PCK1 provides a finely tuned mechanism of controlling glucose metabolism by linking whole nutrient availability to metabolic homeostasis.

Published
2023

22. Analyzing Autophagic Flux in Nerve Cultures

Author

Tamayo Caro, Miguel, primary, Palomo Irigoyen, Marta, additional, Pérez Andrés, Encarnación, additional, Barreira Manrique, Adrián, additional, Varela Rey, Marta, additional, and Woodhoo, Ashwin, additional

Published
2018
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24. Inhibition of carnitine palmitoyltransferase 1A in hepatic stellate cells protects against fibrosis

Author

Fondevila, Marcos F., primary, Fernandez, Uxia, additional, Heras, Violeta, additional, Parracho, Tamara, additional, Gonzalez-Rellan, Maria J., additional, Novoa, Eva, additional, Porteiro, Begoña, additional, Alonso, Cristina, additional, Mayo, Rebeca, additional, da Silva Lima, Natalia, additional, Iglesias, Cristina, additional, Filliol, Aveline A., additional, Senra, Ana, additional, Delgado, Teresa C., additional, Woodhoo, Ashwin, additional, Herrero, Laura, additional, Serra, Dolors, additional, Prevot, Vincent, additional, Schwaninger, Markus, additional, López, Miguel, additional, Dieguez, Carlos, additional, Millet, Oscar, additional, Mato, Jose M., additional, Cubero, Francisco J., additional, Varela-Rey, Marta, additional, Iruzubieta, Paula, additional, Crespo, Javier, additional, Martinez-Chantar, Maria L., additional, Schwabe, Robert F., additional, and Nogueiras, Ruben, additional

Published
2022
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25. Gene Regulatory Network in Schwann cell Disorders

Author

Grandes Moreno, Pedro Rolando, Woodhoo, Ashwin, Neurociencias, Neurozientziak, Palomo Irigoyen, Marta, Grandes Moreno, Pedro Rolando, Woodhoo, Ashwin, Neurociencias, Neurozientziak, and Palomo Irigoyen, Marta

Abstract

222 p., Cancer cells can develop a strong addiction to discrete molecular regulators, which control the aberranttranscription programs that drive and maintain the cancer phenotype. Here, we report the identificationof the RNA-binding protein HuR as a central oncogenic driver for malignant peripheral nerve sheathtumours (MPNSTs), which are highly aggressive sarcomas. We show HuR was bound to a multitude ofcancer-associated transcripts in human MPNST samples. Accordingly, genetic and pharmacologicalinhibition of HuR had potent cytostatic and cytotoxic effects on tumour growth, and strongly supressedmetastatic capacity in vivo. Importantly, we linked the profound tumorigenic function of HuR to itsability to simultaneously regulate multiple essential oncogenic pathways in MPNST cellls, including theWnt/ß-Catenin, YAP/TAZ, Rb-E2F and BET proteins, which converge on key transcriptional networks.Regulating survival, proliferation, and dissemination. Therefore, we propose HuR represents an idealtherapeutic target for MPNST treatment. Peripheral nervous system involvement has also beenobserved in Lyme disease-assicated clinical manifestations, which is the most common arthropod-borne infectious disease in temperate regions. It is caused by infection with the spirochete Borreliaburgdorferi, which is transmitted by a tick bite. Here, we identified Schwann cells as the new targets ofB. burgdorferi, leading to demyelination in the absence of immune cells. We suggest that B. burgdorferiregulates intracellular signaling pathways, including Wnt/ß-Catenin, to strongly repress myelin gene andprotein expression, throwing light on one of the most intriguing pathological features of Lyme disease.

Published
2022

26. Neddylationin Schwann cell myelination

Author

Sierra Saavedra, Amanda, Woodhoo, Ashwin, Neurociencias, Neurozientziak, Tamayo Caro, Miguel, Sierra Saavedra, Amanda, Woodhoo, Ashwin, Neurociencias, Neurozientziak, and Tamayo Caro, Miguel

Abstract

Las secciones 5.13, 5.14,5.15 y 5.16 están sujetas a confidencialidad por el autor. 180 p., En esta tesis, hemos encontrado que la modificación postraduccional llamada neddylationes un regulador crítico de la mielinización en células de Schwann. El silenciamiento genético en célulasde Schwann del gen Nae1 (llamado NAe1 cKO), la enzima que inicia la cascada de neddyaltion, causanotables defectos en los nervios periféricos mostrando síntomas similares a los observados en neuropatías graves. Estos defectos incluyen: la reducción de la velocidad de conducción nerviosa, la ausencia de mielina en los nervios periféricos y la degradación de la mielina en células de Schwann . Al mismo tiempo, pudimos detectar en los ratones silenciados grandes cambios en el perfil transcriptómico y proteómico de las células de Schwann. Estos resultados sugieren que la actividad de las Cullin RingLigases (CRL) podría estar afectada, lo que podría estar impidiendo la degradación de reguladores negativos de la mielinización como c-Jun y Sox2. Por otro lado, no fuimos capaces de relacionar directamente la neddylation con el mantenimiento de las vainas de mielina en ratones adultos ni con papel de las células de Schwann en la regeneración de nervios periféricos en respuesta a daño en los mismos

Published
2022

27. Inhibition of carnitine palmitoyltransferase 1A in hepatic stellate cells protects against fibrosis

Author

Universidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas, Universidade de Santiago de Compostela. Departamento de Fisioloxía, Fernandez Fondevila, Marcos, Fernández Regueira, Uxía, Heras Domínguez, Violeta, Parracho Martínez, Tamara, González Rellán, María Jesús, Nóvoa Deaño, Eva María, Porteiro Couto, Begoña, Alonso, Cristina, Mayo, Rebeca, Silva Lima, Natália da, Iglesias García, Cristina, Filliol, Aveline, Senra Viñas, Ana, Delgado, Teresa, woodhoo, ashwin, Herrero, Laura, Serra, Dolors, Prevot, Vincent, Schwaninger, Markus, López, Miguel, Diéguez González, Carlos, Millet, Óscar, Mato de la Paz, José María, Cubero Palero, Francisco Javier, Varela Rey, Marta, Iruzubieta, Paula, Crespo, Javier, Martinez Chantar, Maria Luz, Schwabe, Robert F., Nogueiras Pozo, Rubén, Universidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas, Universidade de Santiago de Compostela. Departamento de Fisioloxía, Fernandez Fondevila, Marcos, Fernández Regueira, Uxía, Heras Domínguez, Violeta, Parracho Martínez, Tamara, González Rellán, María Jesús, Nóvoa Deaño, Eva María, Porteiro Couto, Begoña, Alonso, Cristina, Mayo, Rebeca, Silva Lima, Natália da, Iglesias García, Cristina, Filliol, Aveline, Senra Viñas, Ana, Delgado, Teresa, woodhoo, ashwin, Herrero, Laura, Serra, Dolors, Prevot, Vincent, Schwaninger, Markus, López, Miguel, Diéguez González, Carlos, Millet, Óscar, Mato de la Paz, José María, Cubero Palero, Francisco Javier, Varela Rey, Marta, Iruzubieta, Paula, Crespo, Javier, Martinez Chantar, Maria Luz, Schwabe, Robert F., and Nogueiras Pozo, Rubén

Abstract

One of the most common types of fibrotic liver occurs in patients with non-alcoholic fatty liver disease (NAFLD), which may develop into non-alcoholic steatohepatitis (NASH). Hepatic stellate cells (HSCs) are the primary fibrogenic cell type activated following liver injury, moving from a quiescent phenotype rich in vitamin A into activated myofibroblast-like cells with proliferative and migratory properties.

Published
2022

28. HuR-targeted agents: An insight into medicinal chemistry, biophysical, computational studies and pharmacological effects on cancer models

Author

Assoni, Giulia, primary, La Pietra, Valeria, additional, Digilio, Rosangela, additional, Ciani, Caterina, additional, Licata, Nausicaa Valentina, additional, Micaelli, Mariachiara, additional, Facen, Elisa, additional, Tomaszewska, Weronika, additional, Cerofolini, Linda, additional, Pérez-Ràfols, Anna, additional, Varela Rey, Marta, additional, Fragai, Marco, additional, Woodhoo, Ashwin, additional, Marinelli, Luciana, additional, Arosio, Daniela, additional, Bonomo, Isabelle, additional, Provenzani, Alessandro, additional, and Seneci, Pierfausto, additional

Published
2022
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30. List of Contributors

Author

Álvarez-Errico, Damiana, primary, Bainor, Anthony J., additional, Ballestar, Esteban, additional, Bayón, Gustavo F., additional, Beekman, Renée, additional, Bibikova, Marina, additional, Bollati, Valentina, additional, Carugno, Michele, additional, Christensen, Brock C., additional, David, Gregory, additional, Fernández, Agustín F., additional, Figueroa, Maria E., additional, Fraga, Mario F., additional, Heyn, Holger, additional, Iodice, Simona, additional, Johnson, Kevin C., additional, Kulis, Marta, additional, Léveillé, Nicolas, additional, Ling, Charlotte, additional, Martín-Subero, José Ignacio, additional, McDevitt, Michael A., additional, Melo, Carlos A., additional, Melo, Sonia A., additional, Motta, Valeria, additional, Park, Yongseok, additional, Qin, Tingting, additional, Rönn, Tina, additional, Sartor, Maureen A., additional, and Woodhoo, Ashwin, additional

Published
2016
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32. HuR/Methyl-HuR and AUF1 Regulate the MAT Expressed During Liver Proliferation, Differentiation, and Carcinogenesis

Author

Vázquez–Chantada, Mercedes, Fernández–Ramos, David, Embade, Nieves, Martínez–Lopez, Nuria, Varela–Rey, Marta, Woodhoo, Ashwin, Luka, Zigmund, Wagner, Conrad, Anglim, Paul P., Finnell, Richard H., Caballería, Juan, Laird–Offringa, Ite A., Gorospe, Myriam, Lu, Shelly C., Mato, José M., and Martínez–Chantar, M. Luz

Published
2010
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33. Alcohol, DNA methylation, and cancer

Author

Varela-Rey, Marta, Woodhoo, Ashwin, Martinez-Chantar, Maria-Luz, Mato, Jose M., and Lu, Shelly C.

Subjects
Alcoholism -- Complications and side effects -- Genetic aspects, DNA -- Health aspects, Methylation -- Health aspects, Carcinogenesis -- Genetic aspects, Genetic polymorphisms -- Health aspects, Cancer -- Risk factors -- Genetic aspects, Oxidative stress -- Genetic aspects -- Health aspects, Epigenetic inheritance -- Health aspects, Alcoholic beverages -- Health aspects -- Genetic aspects, Acetaldehyde -- Genetic aspects -- Health aspects, Health
Abstract

According to the World Health Organization (WHO) Global Burden of Disease Project, alcohol accounts for approximately 3.2 percent of all deaths per year worldwide (corresponding to 1.8 million people) and [...]

Published
2013

34. Hepatic O-GlcNAcylated-p53 as a hub integrating the glucose counterregulatory response and insulin-suppressed gluconeogenesis

Author

González, Rellán María Jesús, primary, Fernández, Fondevila Marcos, additional, Fernández, Paz Uxia, additional, Rodríguez, Amaia, additional, Varela, Rey Marta, additional, Veyrat-Durebex, Christelle, additional, Seoane, Samuel, additional, Bernardo, Ganeko, additional, Fernando, Lopitz Otsoa, additional, Fernandez, Ramos David F, additional, Bilbao, Jon, additional, Iglesias, Cristina, additional, Müller, David F, additional, Herzig, Stephan, additional, Martinez, Chantar Maria Luz, additional, Perez, Fernandez Roman, additional, Lopez, Miguel, additional, Diéguez, Roman, additional, Maria, Mato Jose, additional, Millet, Oscar, additional, Coppari, Roberto, additional, Woodhoo, Ashwin, additional, Fruhbeck, Gema, additional, and Nogueiras, Pozo Rubén, additional

Published
2021
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35. E2F1 and E2F2-mediated repression of CPT2 establishes a lipid-rich tumor-promoting environment

Author

Gonzalez-Romero, Francisco, Mestre, Daniela, Aurrekoetxea, Igor, O'Rourke, Colm J., Andersen, Jesper B., Woodhoo, Ashwin, Tamayo-Caro, Miguel, Varela-Rey, Marta, Palomo-Irigoyen, Marta, Gomez-Santos, Beatriz, de Urturi, Diego Saenz, Nuñez-García, Maitane, García-Rodríguez, Juan L., Fernandez-Ares, Larraitz, Buque, Xabier, Iglesias-Ara, Ainhoa, Bernales, Irantzu, de Juan, Virginia Gutierrez, Delgado, Teresa C., Goikoetxea-Usandizaga, Naroa, Lee, Richard, Bhanot, Sanjay, Delgado, Igotz, Perugorria, Maria J., Errazti, Gaizka, Mosteiro, Lorena, Gaztambide, Sonia, de la Piscina, Idoia Martinez, Iruzubieta, Paula, Crespo, Javier, Banales, Jesus M., Martínez-Chantar, Maria L., Castaño, Luis, Zubiaga, Ana M., Aspichueta, Patricia, Gonzalez-Romero, Francisco, Mestre, Daniela, Aurrekoetxea, Igor, O'Rourke, Colm J., Andersen, Jesper B., Woodhoo, Ashwin, Tamayo-Caro, Miguel, Varela-Rey, Marta, Palomo-Irigoyen, Marta, Gomez-Santos, Beatriz, de Urturi, Diego Saenz, Nuñez-García, Maitane, García-Rodríguez, Juan L., Fernandez-Ares, Larraitz, Buque, Xabier, Iglesias-Ara, Ainhoa, Bernales, Irantzu, de Juan, Virginia Gutierrez, Delgado, Teresa C., Goikoetxea-Usandizaga, Naroa, Lee, Richard, Bhanot, Sanjay, Delgado, Igotz, Perugorria, Maria J., Errazti, Gaizka, Mosteiro, Lorena, Gaztambide, Sonia, de la Piscina, Idoia Martinez, Iruzubieta, Paula, Crespo, Javier, Banales, Jesus M., Martínez-Chantar, Maria L., Castaño, Luis, Zubiaga, Ana M., and Aspichueta, Patricia

Abstract

Lipid metabolism rearrangements in nonalcoholic fatty liver disease (NAFLD) contribute to disease progression. NAFLD has emerged as a major risk for hepatocellular carcinoma (HCC), where metabolic reprogramming is a hallmark. Identification of metabolic drivers might reveal therapeutic targets to improve HCC treatment. Here, we investigated the contribution of transcription factors E2F1 and E2F2 to NAFLD-related HCC and their involvement in metabolic rewiring during disease progression. In mice receiving a high-fat diet (HFD) and diethylnitrosamine (DEN) administration, E2f1 and E2f2 expressions were increased in NAFLD-related HCC. In human NAFLD, E2F1 and E2F2 levels were also increased and positively correlated. E2f1-/- and E2f2-/- mice were resistant to DEN-HFD-induced hepatocarcinogenesis and associated lipid accumulation. Administration of DEN-HFD in E2f1-/- and E2f2-/- mice enhanced fatty acid oxidation (FAO) and increased expression of Cpt2, an enzyme essential for FAO, whose downregulation is linked to NAFLD-related hepatocarcinogenesis. These results were recapitulated following E2f2 knockdown in liver, and overexpression of E2f2 elicited opposing effects. E2F2 binding to the Cpt2 promoter was enhanced in DEN-HFD-administered mouse livers compared with controls, implying a direct role for E2F2 in transcriptional repression. In human HCC, E2F1 and E2F2 expressions inversely correlated with CPT2 expression. Collectively, these results indicate that activation of the E2F1-E2F2-CPT2 axis provides a lipid-rich environment required for hepatocarcinogenesis.

Published
2021

37. E2F1 and E2F2-Mediated Repression of CPT2 Establishes a Lipid-Rich Tumor-Promoting Environment

Author

González-Romero, Francisco, primary, Mestre, Daniela, additional, Aurrekoetxea, Igor, additional, O'Rourke, Colm J., additional, Andersen, Jesper B., additional, Woodhoo, Ashwin, additional, Tamayo-Caro, Miguel, additional, Varela-Rey, Marta, additional, Palomo-Irigoyen, Marta, additional, Gómez-Santos, Beatriz, additional, de Urturi, Diego Sáenz, additional, Núñez-García, Maitane, additional, García-Rodríguez, Juan L., additional, Fernández-Ares, Larraitz, additional, Buqué, Xabier, additional, Iglesias-Ara, Ainhoa, additional, Bernales, Irantzu, additional, De Juan, Virginia Gutierrez, additional, Delgado, Teresa C., additional, Goikoetxea-Usandizaga, Naroa, additional, Lee, Richard, additional, Bhanot, Sanjay, additional, Delgado, Igotz, additional, Perugorria, Maria J., additional, Errazti, Gaizka, additional, Mosteiro, Lorena, additional, Gaztambide, Sonia, additional, Martinez de la Piscina, Idoia, additional, Iruzubieta, Paula, additional, Crespo, Javier, additional, Banales, Jesus M., additional, Martínez-Chantar, Maria L., additional, Castaño, Luis, additional, Zubiaga, Ana M., additional, and Aspichueta, Patricia, additional

Published
2021
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38. SAT-275 - Functional and mechanistic role of lncRNA-A1 in cholangiocarcinoma

Author

Tinahones, Alberto, Vicens, Joan Blázquez, Cañas, Jorge, Capelo, Alba, Lavin, JL, Perugorria Montiel, Maria Jesus, de Barrena, Maite García-Fernandez, Fondevila, Marcos Fernandez, Porteiro, Begoña, Lozano, J, Coll, M, Sancho-Bru, Pau, Gonzalez-Sanchez, Ester, Bañales, Jesús, Milkiewicz, Piotr, Milkiewicz, Malgorzata, Bujanda, Luis, Avila, Ma, Aransay, Ana María, Nogueiras, Ruben, Turnes, Juan, Pelacho, Beatriz, Amengual, J, Fabregat, Isabel, Vaquero, Javier, Entrialgo, Rodrigo, Vicent, Silve, Woodhoo, Ashwin, and Varela-Rey, Marta

Published
2023
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39. WED-223 - Functional and mechanistic role of lncRNA-C1 in cholestatic liver diseases

Author

Vicens, Joan Blázquez, Ruano, Alberto Tinahones, Cañas, Jorge, Borzacchiello, Luigi, Capelo, Alba, Fondevila, Marcos Fernandez, Porteiro, Begoña, Bunyan-Woodcraft, Mason, Ramos, David Fernández, Perugorria Montiel, Maria Jesus, Garcia Pagan, Juan Carlos, Banales, Jesus Maria, Milkiewicz, Piotr, Milkiewicz, Małgorzata, Sangro, Bruno, Bujanda, Luis, Avila, Matías A, Aransay, Ana María, Mato, José M., Turnes, Juan, Pelacho, Beatriz, Nogueiras, Ruben, Martínez-Chantar, María Luz, Woodhoo, Ashwin, and Varela-Rey, Marta

Published
2023
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40. HuR/ELAVL1 drives malignant peripheral nerve sheath tumor growth and metastasis

Author

Bioquímica y biología molecular, Biokimika eta biologia molekularra, Palomo Irigoyen, Marta, Pérez Andrés, Encarni, Iruarrizaga Lejarreta, Marta, Barreira Manrique, Adrián, Tamayo Caro, Miguel, Vila Vecilla, Laura, Moreno Cugnon, Leire, Beitia, Nagore, Medrano, Daniela, Fernández Ramos, David, Lozano, Juan José, Okawa, Satoshi, Lavín, José L., Martín Martín, Natalia, Sutherland, James D., Guitiérez de Juan, Virginia, González López, Monika, Macías Cámara, Nuria, Mosén Ansorena, David, Laraba, Liyam, Hanemann, C. Oliver, Ercolano, Emanuela, Parkinson, David B., Schultz, Christopher W., Araúzo Bravo, Marcos J., Ascensión, Alex M., Gerovska, Daniela, Iribar, Haizea, Izeta, Ander, Pytel, Peter, Krastel, Philipp, Provenzani, Alessandro, Seneci, Pierfausto, Carrasco, Ruben D., Del Sol, Antonio, Martínez Chantar, María Luz, Barrio Olano, María Rosa, Serra, Eduard, Lazaro, Conxi, Flanagan, Adrienne M., Gorospe, Myriam, Ratner, Nancy, Aransay Bañares, Ana María, Carracedo Pérez, Arkaitz, Varela Rey, Marta, Woodhoo, Ashwin, Bioquímica y biología molecular, Biokimika eta biologia molekularra, Palomo Irigoyen, Marta, Pérez Andrés, Encarni, Iruarrizaga Lejarreta, Marta, Barreira Manrique, Adrián, Tamayo Caro, Miguel, Vila Vecilla, Laura, Moreno Cugnon, Leire, Beitia, Nagore, Medrano, Daniela, Fernández Ramos, David, Lozano, Juan José, Okawa, Satoshi, Lavín, José L., Martín Martín, Natalia, Sutherland, James D., Guitiérez de Juan, Virginia, González López, Monika, Macías Cámara, Nuria, Mosén Ansorena, David, Laraba, Liyam, Hanemann, C. Oliver, Ercolano, Emanuela, Parkinson, David B., Schultz, Christopher W., Araúzo Bravo, Marcos J., Ascensión, Alex M., Gerovska, Daniela, Iribar, Haizea, Izeta, Ander, Pytel, Peter, Krastel, Philipp, Provenzani, Alessandro, Seneci, Pierfausto, Carrasco, Ruben D., Del Sol, Antonio, Martínez Chantar, María Luz, Barrio Olano, María Rosa, Serra, Eduard, Lazaro, Conxi, Flanagan, Adrienne M., Gorospe, Myriam, Ratner, Nancy, Aransay Bañares, Ana María, Carracedo Pérez, Arkaitz, Varela Rey, Marta, and Woodhoo, Ashwin

Abstract

Cancer cells can develop a strong addiction to discrete molecular regulators, which control the aberrant gene expression programs that drive and maintain the cancer phenotype. Here, we report the identification of the RNA-binding protein HuR/ELAVL1 as a central oncogenic driver for malignant peripheral nerve sheath tumors (MPNSTs), which are highly aggressive sarcomas that originate from cells of the Schwann cell lineage. HuR was found to be highly elevated and bound to a multitude of cancer-associated transcripts in human MPNST samples. Accordingly, genetic and pharmacological inhibition of HuR had potent cytostatic and cytotoxic effects on tumor growth, and strongly suppressed metastatic capacity in vivo. Importantly, we linked the profound tumorigenic function of HuR to its ability to simultaneously regulate multiple essential oncogenic pathways in MPNST cells, including the Wnt/beta-catenin, YAP/TAZ, RB/E2F, and BET pathways, which converge on key transcriptional networks. Given the exceptional dependency of MPNST cells on HuR for survival, proliferation, and dissemination, we propose that HuR represents a promising therapeutic target for MPNST treatment.

Published
2020

41. Gene Regulatory Network in Schwann cell Disorders

Author

Grandes Moreno, Pedro Rolando, Woodhoo, Ashwin, Neurociencias, Neurozientziak, Palomo Irigoyen, Marta, Grandes Moreno, Pedro Rolando, Woodhoo, Ashwin, Neurociencias, Neurozientziak, and Palomo Irigoyen, Marta

Abstract

222 p., Cancer cells can develop a strong addiction to discrete molecular regulators, which control the aberranttranscription programs that drive and maintain the cancer phenotype. Here, we report the identificationof the RNA-binding protein HuR as a central oncogenic driver for malignant peripheral nerve sheathtumours (MPNSTs), which are highly aggressive sarcomas. We show HuR was bound to a multitude ofcancer-associated transcripts in human MPNST samples. Accordingly, genetic and pharmacologicalinhibition of HuR had potent cytostatic and cytotoxic effects on tumour growth, and strongly supressedmetastatic capacity in vivo. Importantly, we linked the profound tumorigenic function of HuR to itsability to simultaneously regulate multiple essential oncogenic pathways in MPNST cellls, including theWnt/ß-Catenin, YAP/TAZ, Rb-E2F and BET proteins, which converge on key transcriptional networks.Regulating survival, proliferation, and dissemination. Therefore, we propose HuR represents an idealtherapeutic target for MPNST treatment. Peripheral nervous system involvement has also beenobserved in Lyme disease-assicated clinical manifestations, which is the most common arthropod-borne infectious disease in temperate regions. It is caused by infection with the spirochete Borreliaburgdorferi, which is transmitted by a tick bite. Here, we identified Schwann cells as the new targets ofB. burgdorferi, leading to demyelination in the absence of immune cells. We suggest that B. burgdorferiregulates intracellular signaling pathways, including Wnt/ß-Catenin, to strongly repress myelin gene andprotein expression, throwing light on one of the most intriguing pathological features of Lyme disease.

Published
2020

43. Murine double minute 2 regulates Hu antigen R stability in human liver and colon cancer through NEDDylation

Author

Embade, Nieves, Fernández-Ramos, David, Varela-Rey, Marta, Beraza, Naiara, Sini, Marcella, de Juan, Virginia Gutiérrez, Woodhoo, Ashwin, Martínez-López, Nuria, Rodríguez-Iruretagoyena, Begoña, Bustamante, Francisco Javier, de la Hoz, Ana Belén, Carracedo, Arkaitz, Xirodimas, Dimitris P., Rodríguez, Manuel S., Lu, Shelly C., Mato, José M., and Martínez-Chantar, María L.

Published
2012
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45. HuR/ELAVL1 drives malignant peripheral nerve sheath tumor growth and metastasis

Author

Palomo-Irigoyen, Marta, primary, Pérez-Andrés, Encarni, additional, Iruarrizaga-Lejarreta, Marta, additional, Barreira-Manrique, Adrián, additional, Tamayo-Caro, Miguel, additional, Vila-Vecilla, Laura, additional, Moreno-Cugnon, Leire, additional, Beitia, Nagore, additional, Medrano, Daniela, additional, Fernández-Ramos, David, additional, Lozano, Juan José, additional, Okawa, Satoshi, additional, Lavín, José L., additional, Martín-Martín, Natalia, additional, Sutherland, James D., additional, de Juan, Virginia Guitiérez, additional, Gonzalez-Lopez, Monika, additional, Macías-Cámara, Nuria, additional, Mosén-Ansorena, David, additional, Laraba, Liyam, additional, Hanemann, C. Oliver, additional, Ercolano, Emanuela, additional, Parkinson, David B., additional, Schultz, Christopher W., additional, Araúzo-Bravo, Marcos J., additional, Ascensión, Alex M., additional, Gerovska, Daniela, additional, Iribar, Haizea, additional, Izeta, Ander, additional, Pytel, Peter, additional, Krastel, Philipp, additional, Provenzani, Alessandro, additional, Seneci, Pierfausto, additional, Carrasco, Ruben D., additional, Del Sol, Antonio, additional, Martinez-Chantar, María Luz, additional, Barrio, Rosa, additional, Serra, Eduard, additional, Lazaro, Conxi, additional, Flanagan, Adrienne M., additional, Gorospe, Myriam, additional, Ratner, Nancy, additional, Aransay, Ana M., additional, Carracedo, Arkaitz, additional, Varela-Rey, Marta, additional, and Woodhoo, Ashwin, additional

Published
2020
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46. Activation of LKB1-Akt Pathway Independent of Phosphoinositide 3-Kinase Plays a Critical Role in the Proliferation of Hepatocellular Carcinoma From Nonalcoholic Steatohepatitis

Author

Martínez-López, Nuria, Varela-Rey, Marta, Fernández-Ramos, David, Woodhoo, Ashwin, Vázquez-Chantada, Mercedes, Embade, Nieves, Espinosa-Hevia, Luis, Bustamante, Francisco Javier, Parada, Luis A., Rodriguez, Manuel S., Lu, Shelly C., Mato, José M., and Martínez-Chantar, Maria L.

Published
2010
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