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Abstract The screening of siRNAs targeting 390 human G protein-coupled receptors (GPCRs) was multiplexed in combination with cisplatin against lung cancer cells. While the cell viability measure hardly captured the anticancer effect of siGPCRs, the direct cell count revealed the anticancer potential of diverse GPCRs (46 hits with > twofold growth inhibition, p-value

ARL2 regulates the dynamics of cytological components and is highly expressed in colon cancer tissues. Here, we report novel roles of ARL2 in the cell nucleus and colon cancer stem cells (CSCs). ARL2 is expressed at relatively low levels in K‐RAS active colon cancer cells, but its expression is induced in CSCs. Depletion of ARL2 results in M phase arrest exclusively in non‐CSC cultured cells; in addition, DNA break stress accumulates in CSCs leading to apoptosis. ARL2 expression is positively associated with the expression of all six RAD51 family genes, which are essential for homologous recombination repair (HRR). Furthermore, ARL2 is required for HRR and detected within chromatin compartments. These results demonstrate the requirement of ARL2 in colon CSC maintenance, which possibly occurs through mediating double‐strand break DNA repair in the nucleus.

Abstract The success of cancer chemotherapy is limited by multidrug resistance (MDR), which is mainly caused by P-glycoprotein (P-gp) overexpression. In the present study, we describe a novel microtubule inhibitor, 5-(N-methylmaleimid-3-yl)-chromone (SPC-160002), that can be used to overcome MDR. A synthetic chromone derivative, SPC-160002, showed a broad spectrum of anti-proliferative effects on various human cancer cells without affecting P-gp expression and its drug efflux function. Treatment with SPC-160002 arrested the cell cycle at the M phase, as evidenced using fluorescence-activated cell sorting analysis, and increased the levels of mitotic marker proteins, including cyclin B, pS10-H3, and chromosomal passenger complex. This mitotic arrest by SPC-160002 was mediated by promoting and stabilizing microtubule polymerization, similar to the mechanism observed in case of taxane-based drugs. Furthermore, SPC-160002 suppressed the growth and sphere-forming activity of cancer stem cells. Our data herein strongly suggest that SPC-160002, a novel microtubule inhibitor, can be used to overcome MDR and can serve as an attractive candidate for anticancer drugs.

Background: As a process of aging, skeletal muscle mass and function gradually decrease. It is reported that ginsenoside Rb1 and Rb2 play a role as AMP-activated protein kinase activator, resulting in regulating glucose homeostasis, and Rb1 reduces oxidative stress in aged skeletal muscles through activating the phosphatidylinositol 3-kinase/Akt/Nrf2 pathway. We examined the effects of Rb1 and Rb2 on differentiation of the muscle stem cells and myotube formation. Methods: C2C12 myoblasts treated with Rb1 and/or Rb2 were differentiated and induced to myotube formation, followed by immunoblotting for myogenic marker proteins, such as myosin heavy chain, MyoD, and myogenin, or immunostaining for myosin heavy chain or immunoprecipitation analysis for heterodimerization of MyoD/E-proteins. Results: Rb1 and Rb2 enhanced myoblast differentiation through accelerating MyoD/E-protein heterodimerization and increased myotube hypertrophy, accompanied by activation of Akt/mammalian target of rapamycin signaling. In addition, Rb1 and Rb2 induced the MyoD-mediated transdifferentiation of the rhabdomyosarcoma cells into myoblasts. Furthermore, co-treatment with Rb1 and Rb2 had synergistically enhanced myoblast differentiation through Akt activation. Conclusion: Rb1 and Rb2 upregulate myotube growth and myogenic differentiation through activating Akt/mammalian target of rapamycin signaling and inducing myogenic conversion of fibroblasts. Thus, our first finding indicates that Rb1 and Rb2 have strong potential as a helpful remedy to prevent and treat muscle atrophy, such as age-related muscular dystrophy. Keywords: Akt/mTOR signaling, Hypertrophy, Myoblast differentiation, Rb1, Rb2

Background and Objectives: Prion diseases are fatal neurodegenerative disorders caused by the abnormal proteinase K-resistant prion protein (PrPSc). Since variant Creutzfeldt–Jakob disease (CJD) was first reported in the United Kingdom (UK) in 1996, the occurrence of variant CJD has been reported in over 10 countries. To date, variant CJD has not been reported in Korea. However, the E211K somatic mutation in the prion protein gene (PRNP), which is related to bovine spongiform encephalopathy (BSE), was reported in Korean Holstein cattle, and atypical BSE, which is supposed to be sporadic BSE, has been occurring in many countries, including Japan and the USA. These results suggest that BSE may occur naturally in Korea. Thus, we performed a preemptive PrPSc test in appendix specimens to diagnose variant CJD in a Korean population. Materials and Methods: In the present study, we investigated CJD-related mutations and polymorphisms of the PRNP gene and carried out an examination on PrPSc in appendix specimens of Korean patients after appendectomy. Results: In all Korean appendix specimens tested, PrPSc bands were not detected. Conclusion: To the best of our knowledge, this was the first evaluation of PrPSc in Korean appendix specimens.

A memristor is a fundamental electronic device that operates like a biological synapse and is considered as the solution of classical von Neumann computers. Here, a fully printed and flexible memristor is fabricated by depositing a thin film of metal–non-metal (chromium-nitrogen)-doped titanium dioxide (TiO2). The resulting device exhibited enhanced performance with self-rectifying and forming free bipolar switching behavior. Doping was performed to bring stability in the performance of the memristor by controlling the defects and impurity levels. The forming free memristor exhibited characteristic behavior of bipolar resistive switching with a high on/off ratio (2.5 × 103), high endurance (500 cycles), long retention time (5 × 103 s) and low operating voltage (±1 V). Doping the thin film of TiO2 with metal–non-metal had a significant effect on the switching properties and conduction mechanism as it directly affected the energy bandgap by lowering it from 3.2 eV to 2.76 eV. Doping enhanced the mobility of charge carriers and eased the process of filament formation by suppressing its randomness between electrodes under the applied electric field. Furthermore, metal–non-metal-doped TiO2 thin film exhibited less switching current and improved non-linearity by controlling the surface defects.

Two-dimensional (2D) materials and their composites have gained significant importance as the functional layer of various environmental sensors and nanoelectronics owing to their unique properties. This work reports for the first time a highly sensitive, fast, and stable humidity sensor based on the bi-layered active sensing area composed of graphene flower (GF) and poly (vinyl alcohol) PVA thin films for multifunctional applications. The GF/PVA humidity sensor exhibited stable impedance response over 15 days, for a relative humidity (RH) range of (40–90% RH) under ambient operating conditions. The proposed bi-layered humidity sensor also exhibited an ultra-high capacitive sensitivity response of the 29 nF/%RH at 10 kHz and fast transient response of 2 s and 3.5 s, respectively. Furthermore, the reported sensor also showed a good response towards multi-functional applications such as non-contact skin humidity and mouth breathing detection.

TiO2-based materials are commonly employed as photocatalysts for industrial wastewater treatment. The primary reasons of employing TiO2 include cost effectiveness, ready availability, eco-friendliness, non-toxic behavior, and exceptional resistance towards photo-corrosion. However, the wider band gap of pure TiO2 restricts its performance because of its optical absorption of solar light to the ultraviolet (UV) region only, and to some extent of photo-excited charge recombination. In the present work an attempt is made to develop a facile synthesis approach by using urea, a cheap chemical precursor, to form nitrogen doped TiO2 with the key objective of extended light absorption and thus enhanced photocatalytic performance. It was also observed that the urea-induced anatase phase enrichment of TiO2 is another key factor in promoting the photocatalytic performance. The photocatalysts prepared by varying the amount of urea as a nitrogen dopant precursor, are characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, and photoluminescence (PL) to evaluate their crystallinity, morphology, functional groups, and charge separation properties, respectively. Moreover, the surface area was also estimated by physicochemical adsorption. The maximum nitrogen-doped sample yielded >99% photodegradation efficiency of methylene blue (MB) dye-simulated wastewater as compared to a pure TiO2 sample which exhibited 6.46% efficiency. The results show that the simultaneous factors of nitrogen doping and anatase phase enhancement contributes significantly towards the improvement of photocatalytic performance.

Biopolymers are a solution to solve the increasing problems caused by the advances and revolution in the electronic industry owing to the use of hazardous chemicals. In this work, we have used egg white (EW) as the low-cost functional layer of a biocompatible humidity sensor and deposited it on gold (Au) interdigitated electrodes (IDEs) patterned through the state-of-the-art fabrication technology of thermal vacuum evaporation. The presence of hydrophilic proteins inside the thin film of EW makes it an attractive candidate for sensing humidity. Usually, the dependence of the percentage of relative humidity (%RH) on the reliability of measurement setup is overlooked for impedimetric humidity sensors but we have used a modified experimental setup to enhance the uniformity of the obtained results. The characteristics of our device include almost linear response with a quick response time (1.2 s) and fast recovery time (1.7 s). High sensitivity of 50 kΩ/%RH was achieved in the desirable detection range of 10–85%RH. The device size was intentionally kept small for its potential integration in a marketable chip. Results for the response of our fabricated sensor for dry and wet fingertips, along with determining the rate of breathing through the mouth, are part of this study, making it a potential device for health monitoring.

A novel composite based on a polymer (P(VDF-TrFE)) and a two-dimensional material (graphene flower) was proposed as the active layer of an interdigitated electrode (IDEs) based humidity sensor. Silver (Ag) IDEs were screen printed on a flexible polyethylene terephthalate (PET) substrate followed by spin coating the active layer of P(VDF-TrFE)/graphene flower on its surface. It was observed that this sensor responds to a wide relative humidity range (RH%) of 8–98% with a fast response and recovery time of 0.8 s and 2.5 s for the capacitance, respectively. The fabricated sensor displayed an inversely proportional response between capacitance and RH%, while a directly proportional relationship was observed between its impedance and RH%. P(VDF-TrFE)/graphene flower-based flexible humidity sensor exhibited high sensitivity with an average change of capacitance as 0.0558 pF/RH%. Stability of obtained results was monitored for two weeks without any considerable change in the original values, signifying its high reliability. Various chemical, morphological, and electrical characterizations were performed to comprehensively study the humidity-sensing behavior of this advanced composite. The fabricated sensor was successfully used for the applications of health monitoring and measuring the water content in the environment.

The elimination of the cancer stem cell (CSC) population may be required to achieve better outcomes of cancer therapy. We evaluated stearoyl-CoA desaturase 1 (SCD1) as a novel target for CSC-selective elimination in colon cancer. CSCs expressed more SCD1 than bulk cultured cells (BCCs), and blocking SCD1 expression or function revealed an essential role for SCD1 in the survival of CSCs, but not BCCs. The CSC potential selectively decreased after treatment with the SCD1 inhibitor in vitro and in vivo. The CSC-selective suppression was mediated through the induction of apoptosis. The mechanism leading to selective CSC death was investigated by performing a quantitative RT-PCR analysis of 14 CSC-specific signaling and marker genes after 24 and 48 h of treatment with two concentrations of an inhibitor. The decrease in the expression of Notch1 and AXIN2 preceded changes in the expression of all other genes, at 24 h of treatment in a dose-dependent manner, followed by the downregulation of most Wnt- and NOTCH-signaling genes. Collectively, we showed that not only Wnt but also NOTCH signaling is a primary target of suppression by SCD1 inhibition in CSCs, suggesting the possibility of targeting SCD1 against colon cancer in clinical settings.

This case report discusses a patient diagnosed with L1 burst fracture, right lateral femoral condyle and proximal tibial and fibular head and neck comminuted fractures, and left medial femoral condyle and suspected tibial fractures after a traffic accident. She received integrated Korean medicine treatment, including motion- style acupuncture treatment (MSAT), electroacupuncture, acupuncture, herbal medicine, moxibustion, and cupping therapy for 112 days. The scores improved for the low back pain Numeric Rating Scale (NRS) (from 7 to 3), Oswestry Disability Index (from 94 to 38), bilateral leg pain NRS (from 6 to 2), Lysholm Knee Scoring Scale (from 0 to 66), left ankle manual muscle test (from Poor− to Normal), Korean version of the modified Barthel index (from 32 to 92), and EuroQol 5-dimension 5-level (from 25 to 9), as did mobility. Radiological scans also showed fracture healing. This study suggests the potential of MSAT in high-energy polytrauma care, particularly for fractures.

Background: Preservation of the external branch of the superior laryngeal nerve (EBSLN) during thyroidectomy is important because its injury may lead to frequent occurrence of vocal fatigue and the inability to perform superenergetic phonation. Most studies on the anatomy of EBSLN have been performed in Western countries and, thus, have some limitations in their application to Koreans. We explored the anatomy of EBSLNs using intraoperative neuromonitoring (IONM) in Korean adults. Methods: Between August 2011 and December 2011, 50 patients underwent thyroidectomy at Korea University, Anam Hospital in Seoul, Korea. IONM was performed with the NIM response 3.0 system. Results: Forty-two total thyroidectomies and eight lobectomies were performed with IONM and 92 EBSLNs were evaluated. Type I EBSLN was observed in 15 of the 92 nerves (16.3%), type IIa EBSLN was noted in 52 (56.5%) and type IIb EBSLN was noted in 25 (27.2%). Patients with types IIa and IIb were at higher risk of injuries and these types were more frequently observed (83.7%) compared with previous Western studies. We found that 35.9% of distal insertion sites of EBSLNs were located within 1 cm of the center of the cricoid cartilage. Conclusion: Surgeons should pay close attention to preserving the EBSLN during thyroidectomy in Korean patients because their EBSLNs are more frequently located beneath the superior thyroid vessels.

A crucial tradeoff between material efficacy and environmental impact is often encountered in the development of high‐performance sensors. The use of rare‐earth elements or intricate fabrication techniques is sometimes needed for conventional sensing materials, posing concerns regarding sustainability. Exploring the potential of tomato peel (TP) as a dual‐purpose sensing dielectric layer for pressure and humidity monitoring is a paradigm shift, capitalizing on its porous structure and hygroscopic nature. TP‐based humidity sensor (TP‐HS) exhibits impressive results, with a wide humidity sensing range (5%–95%), fast response/recovery time (6.5/9 s), a high sensitivity (12 500 pF %RH−1), and a high stability (30 days). Additionally, TP‐based pressure sensor (TP‐PS) also shows excellent performance in accurately sensing pressure changes in a wide range (0–196 kPa). TP‐HS can easily distinguish between breathing rates (normal, fast, and slow) and moisture content present in different moisturizers (aloe vera and sanitizer) along with its successful use for proximity sensing. Alternatively, TP‐PS demonstrates weight measurement (490 and 980 N), grip recognition (measuring the pressure exerted by each finger), and gesture detection (by monitoring multiple bending angles 0°, 30°, 50°, and 80°). A wearable, biocompatible dual sensor based on a promising sustainable material for environmental, robotic, and health monitoring applications is successfully demonstrated.

Among the many stilbenoids found in a variety of berries, resveratrol and pterostilbene are of particular interest given their potential for use in cancer therapeutics and prevention. We purified four stilbenoids from R. undulatum and found that pterostilbene inhibits cancer cell proliferation more efficiently than rhapontigenin, piceatannol and resveratrol. To investigate the underlying mechanism of this superior action of pterostilbene on cancer cells, we utilized a reverse-phase protein array followed by bioinformatic analysis and found that the ATM/CHK pathway is modified by pterostilbene in a lung cancer cell line. Given that ATM/CHK signaling requires p53 for its biological effects, we hypothesized that p53 is required for the anticancer effect of pterostilbene. To test this hypothesis, we used two molecularly defined precancerous human bronchial epithelial cell lines, HBECR and HBECR/p53i, with normal p53 and suppressed p53 expression, respectively, to represent premalignant states of squamous lung carcinogenesis. Pterostilbene inhibited the cell cycle more efficiently in HBECR cells compared to HBECR/p53i cells, suggesting that the presence of p53 is required for the action of pterostilbene. Pterostilbene also activated ATM and CHK1/2, which are upstream of p53, in both cell lines, though pterostilbene-induced senescence was dependent on the presence of p53. Finally, pterostilbene more effectively inhibited p53-dependent cell proliferation compared to the other three stilbenoids. These results strongly support the potential chemopreventive effect of pterostilbene on p53-positive cells during early carcinogenesis.

Background: The objectives of this study were to compare the efficacy of acupuncture among different types and to evaluate point-selective pain relief effects between selected adjacent or distant points in participants with temporomandibular joint disorders (TMDs). Methods: Forty-two participants were randomly allocated to three groups: an adjacent point selection group (Trt, n = 14), a distant point selection group (Con1, n = 14), or a combination group (Con2, n = 14). All three groups received a total of six acupuncture sessions (twice a week for 3 weeks), the outcomes being assessed pain intensity using a 10-cm visual analogue scale, and the palpation index of the muscle and temporomandibular joint every week of treatment and 4 weeks after the end of treatment. Results: The pain intensity was reduced in the Trt (34%), Con1 (31%), and Con2 (36%) groups after 3 weeks compared with each group's baseline, with no significant difference among the three groups (p = 0.5867). Similarly, the palpation index was decreased by 52% (Trt), 62% (Con1), and 50% (Con2) after 3 weeks of treatment, but no significant differences between groups were shown (p = 0.3289). Conclusion: Our results suggest that point-selective effects among adjacent, distal, or a combination of acupoints are hardly associated with pain intensity or palpation index in participants with TMDs. Larger sample size trials are required to overcome the shortcomings of the study.

A review of randomized controlled trials (RCTs) using electroacupuncture (EA) to treat patients with foot drop was performed to analyze the effectiveness of EA for this condition. Relevant studies (n = 183) from 7 databases (Cochrane Library, Excerpta Medica Database, PubMed, China National Knowledge Infrastructure, Korean Studies Information Service System, Research Information Sharing Service, and Oriental Medicine Advanced Searching Integrated System) were selected based on the inclusion and exclusion criteria, and 12 RCTs met the selection criteria. In all 12 studies, EA showed significantly positive changes. In most indicators, positive changes were observed in the EA group compared with that in the control group. Significant increases were confirmed in muscle strength-related indicators such as the Fugl–Meyer motor scale, surface electromyography, active range of motion, and gait-related indicators such as the Tinetti score, maximum walking speed, and Berg balance scale. No notable adverse events were reported. EA is suggested as an effective treatment for post-stroke foot drop; however, more RCTs are required.

Abstract The sustained water repellency of interconnected micropatterned surfaces is explored over an extended duration, with a focus on their resilience during a 90‐day water‐immersion test. Initially, the microstructure surfaces exhibit high water repellency, a characteristic of the Cassie–Baxter state. However, subsequent detailed temporal analyses reveal varying responses depending on the structural topology. The interconnected micropatterned surfaces exhibit remarkable long‐term resistance to water; this is attributed to the formation of large and stable air pockets enabled by their unique microcavity structures. In comparison, hierarchical microcavity surfaces with micropillars exhibit a notable decrease in water repellency, as evidenced by reduced contact angles, suggesting a transition to a wetting state owing to the emergence of surface hydrophilicity during long‐term water exposure. This study demonstrates the importance of stable air‐pocket effects, particularly in applications where the long‐term stability of liquid repellency is critical, and suggests the role of interconnected structures in maintaining water repellency over time.

Zinc oxide (ZnO)/titanium dioxide (TiO2) nanorods have been synthesized via a hydrothermal method for ZnO nanorods and an electron-beam deposition for TiO2 nanorods. This work examined the effect of annealing ZnO seed layer on the photocatalytic activity of the ZnO/TiO2 nanorods which was determined from photodecomposition of methylene blue under UV irradiation. The photocatalytic activity of the ZnO/TiO2 nanorods was improved with increasing annealing temperature of the seed layer from 300°C to 500°C. Annealing the seed layer at 500°C showed the best photocatalytic activity resulting from high UV absorption ability, a large surface area with flower structure and copious oxygen defects which promote separation of electron-hole pairs reducing electron recombination. The prepared nanorods were characterized by field emission-scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), photoluminescence (PL), and UV-visible spectroscopy.

Flexible pressure sensors are essential in intelligent skins, healthcare monitoring systems, and soft robotics to bridge mechanical movements and electrical signals. Conventional pressure sensors, however, have a variety of challenges, including a complex fabrication method, increased cost, and biocompatibility issues. This work presents a novel bio-friendly, hierarchically structured porous natural dielectric material based capacitive pressure sensor featuring a maximum sensitivity of 0.0572 kPa−1. The proposed pressure sensor uses a biofilm with a typically arranged honeycomb structure as a dielectric layer sandwiched between the Aluminum (Al) electrodes and incorporates polydimethylsiloxane (PDMS) encapsulation. With a 1.44 cm2 sensing area, the device exhibits a response time (Tres) of 250 ms and a recovery time (Trec) of 156 ms, functioning within a capacitive pressure sensing range of 0–110 kPa. This device monitors various human activities and stimuli and accurately detects pressure distribution and gesture recognition.

Recently graphene, a two-dimensional, layered carbon nanomaterial, has gained attention of the researchers working on nanomaterials due to its outstanding mechanical, thermal, and electrical properties. When incorporated into polymer matrices, graphene serves as a versatile filler, resulting in the development of high-performance nanocomposites with variety of applications. This review article delves into the latest advancements in graphene-based polymer composites, primarily focusing on their role in energy-related applications.The article explores the extensive potential of graphene-based polymer nanocomposites and covers their fabrication using diverse techniques. These techniques include solution mixing, in-situ polymerization, electrospinning, and layer-by-layer assembly, each tailored to meet specific composite requirements. These versatile methods enable researchers to design and engineer graphene-polymer composites with tailored properties.The practical applications of these composites span various fields, with a significant emphasis on energy-related applications. They find utility in energy storage and conversion. This includes the enhancement of supercapacitors, lithium-ion batteries, fuel cells, and electrolyte membranes, all of which benefit from the improved performance and stability imparted by graphene. The article meticulously reviews the latest breakthroughs and innovations in these areas, underscoring the substantial progress achieved by researchers in enhancing the efficiency of graphene-based polymer nanocomposites for energy applications which includes conversion and storage.This review article provides a comprehensive and insightful examination of graphene-based polymer nanocomposites. It covers their fabrication techniques, outlines their applications in energy storage and conversion, and showcases both recent advancements and historical developments. By acknowledging the achievements and considering potential future directions, the article offers a well-rounded perspective on the evolving landscape of energy-related materials science.

To determine the effectiveness of acupuncture in treating restless legs syndrome (RLS), we conducted a literature review of randomized controlled trials (RCTs) that utilized acupuncture as an intervention for patients diagnosed with RLS. Relevant clinical studies (n = 158) from seven databases (the Cochrane Library, PubMed, Embase, CNKI, KISS, RISS, and OASIS) were included based on the inclusion and exclusion criteria and analyzed. Moreover, 6 RCTs were selected for review. In all six studies, it was indicated people who underwent acupuncture treatment showed significant improvements in their overall health. An increase in the treatment efficacy rate, sleep quality, and quality of life indicators after the acupuncture treatment was confirmed. The severity of pain as assessed using the visual analog scale (VAS) scores and International RLS Study Group Rating Scale (IRLSRS) scores and the severity of RLS symptoms were significantly reduced. Any significant side effects were not reported. Acupuncture is suggested as an effective and safe treatment method for RLS. However, further large-scale RCT studies are needed to confirm our findings.

Body balancing is a complex task that includes the coordination of muscles, tendons, bones, ears, eyes, and the brain. Imbalance or disequilibrium is the inability to maintain the center of gravity. Perpetuating body balance plays an important role in preventing us from falling or swaying. Biomechanical tests and video analysis can be performed to analyze body imbalance. The musculoskeletal system is one of the fundamental systems by which our balance or equilibrium is sustained and our upright posture is maintained. Electromyogram (EMG) and ground reaction force (GRF) monitoring can be utilized in cases where a rapid response to body imbalance is a necessity. Body balance also depends on visual stimuli that can be either real or virtual. Researchers have used virtual reality (VR) to predict motion sickness and analyze heart rate variability, as well as in rehabilitation. VR can also be used to induce body imbalance in a controlled way. In this research, body imbalance was induced in a controlled way by playing an Oculus game and, simultaneously, EMG and GRF were recorded. Features were extracted from the EMG and were then fed to a machine learning algorithm. Several machine learning algorithms were tested and upon 10-fold cross-validation; a minimum accuracy of 71% and maximum accuracy of 98% were achieved by Gaussian Naïve Bayes and Gradient Boosting classifiers, respectively, in the classification of imbalance and its intensities. This research can be incorporated into various rehabilitative and therapeutic systems.

Diagnosis of bruxism is challenging because not all contractions of the masticatory muscles can be classified as bruxism. Conventional methods for sleep bruxism detection vary in effectiveness. Some provide objective data through EMG, ECG, or EEG; others, such as dental implants, are less accessible for daily practice. These methods have targeted the masseter as the key muscle for bruxism detection. However, it is important to consider that the temporalis muscle is also active during bruxism among masticatory muscles. Moreover, studies have predominantly examined sleep bruxism in the supine position, but other anatomical positions are also associated with sleep. In this research, we have collected EMG data to detect the maximum voluntary contraction of the temporalis and masseter muscles in three primary anatomical positions associated with sleep, i.e., supine and left and right lateral recumbent positions. A total of 10 time domain features were extracted, and six machine learning classifiers were compared, with random forest outperforming others. The models achieved better accuracies in the detection of sleep bruxism with the temporalis muscle. An accuracy of 93.33% was specifically found for the left lateral recumbent position among the specified anatomical positions. These results indicate a promising direction of machine learning in clinical applications, facilitating enhanced diagnosis and management of sleep bruxism.

Pharmaceutical industry wastewater is causing an increased risk of resistant pharmaceutical micropollutants (PMP) e.g. antibiotic resistant bacteria (super bug) in the ecosystem. Amongst variety of wastewater treatment approaches, Advanced oxidation processes (AOPs) employing photocatalysis provides a cost-effective and sustainable approach for fixation of PMP in an economical and efficient manner to counter the potential risks. Until today, tremendous efforts have been made to trig the performance of photocatalytic wastewater treatment, with the key focus on development of cost-effective, efficient and a moderately stable photocatalyst. Such attempts succeeded with different types of photocatalysts using different synthesis techniques. In recent years, graphitic carbon nitride (GCN) has emerged as one of the cost effective, moderately stable, nontoxic and efficient photocatalyst, and has been scarcely studied specifically for pharmaceutical micropollutants (PMPs) degradation. Hence, considering these factors alongside the facile synthesis and moderate optical absorption of GCN, an effort was made in the present work with effective customization of GCN i.e. silver (Ag) doping to extend light absorption in visible light range which may enhance the photocatalytic performance for Ciprofloxacin (CIP) degradation. The optimization of photocatalytic performance was executed with varied Ag dopant content to obtain an optimum sample with supreme photocatalytic activity for the maximum degradation of CIP, a common antibiotic. The best Ag-doped GCN sample (0.1 AGCN) exhibits a photocatalytic degradation efficiency of 84%, which is 2.15 times greater than pure GCN (39%). The obtained results showed that the strategy of Ag doping substantially enhances the photocatalytic performance, thus offering an efficient mean for developing visible light active photocatalyst for PMP removal and encouraging further research. The photocatalytic performance of the prepared samples was evaluated by degradation of CIP under visible light irradiation. Several characterization techniques were used to characterize and analyze the prepared samples, such as X-ray diffraction (XRD), Scanning electron microscopy (SEM), Raman spectroscopy, Fourier Transform Infrared (FTIR) spectroscopy, UV Visible absorption, and Photoluminescence (PL) spectroscopy.

An efficient rare earth metal (nickel) doped graphitic carbon nitride photocatalyst is developed by a facile synthesis approach. The synthesized nickel doped graphitic carbon nitride (x-NGCN) is employed for photocatalytic degradation of organic dye methylene blue (MB) simulated textile wastewater. It is observed that Ni doping of GCN enhances the optical absorption to such an extent that it efficiently degrades the MB dye under simple LED white light (5 W), mainly consisting of visible light range. Hence Ni doping resulted in sensitivity towards visible light range of spectrum due to narrowing of bandgap, thus increasing the photocatalytic performance. The prepared photocatalysts are characterized using various spectroscopy tools to investigate the crystallinity, morphology, functional groups, optical absorption, and charge separation efficiency.

Abstract The main challenge in preparing a flexible mold stamp using roll-to-roll nanoimprint lithography is to simultaneously increase the imprintable area with a minimized perceptible seam. However, the current methods for stitching multiple small molds to fabricate large-area molds and functional surfaces typically rely on the alignment mark, which inevitably produces a clear alignment mark and stitched seam. In this study, we propose a mark-less alignment by the pattern itself method inspired by moiré technique, which uses the Fourier spectral analysis of moiré patterns formed by superposed identical patterns for alignment. This method is capable of fabricating scalable functional surfaces and imprint molds with quasi-seamless and alignment mark-free patterning. By harnessing the rotational invariance property in the Fourier transform, our approach is confirmed to be a simple and efficient method for extracting the rotational and translational offsets in overlapped periodic or nonperiodic patterns with a minimized stitched region, thereby allowing for the large-area and quasi-seamless fabrication of imprinting molds and functional surfaces, such as liquid-repellent film and micro-optical sheets, that surpass the conventional alignment and stitching limits and potentially expand their application in producing large-area metasurfaces.

Biomaterials will have far reaching effects on solving the ever-rising environmental problems caused by the presently used toxic materials. Disposal of hazardous materials based electronic devices is causing a huge environmental problem in the form of electronic wastes (E-wastes). This study is an effort to contribute in the reduction of E-wastes by presenting the design, fabrication, and performance evaluation of a fully biocompatible humidity sensor with gelatin as the active layer. Gelatin is an excellent choice for humidity sensing because it is a hygroscopic material with the natural ability to attract and absorb water molecules, allowing for an effective detection and measurement of humidity levels in its surrounding environment. We patterned highly conductive (2.05 μΩ-cm) gold (Au) interdigitated electrode (IDE) (50 nm thickness) structure on a glass substrate by using thermal vacuum evaporation followed by the deposition of gelatin thin film by using cost effective technique of spin coating (at 1,500 rpm for 15 s). The device size was 25 mm × 22 mm with a line spacing of 1.50 mm between IDEs. Electrical characterisation was carried out to determine necessary performance parameters of the fully biocompatible humidity sensor and the obtained results exhibited a fast response/recovery time (4/6.30 s), wide operating range (15 %RH to 86 %RH), highly repeatable behaviour of 3 different samples and long lifetime (1 month). Wide variation in the resistance of sensor was observed at extreme humidity sensors ranging from 2.45 MΩ (15 %RH) to 0.12 MΩ (86 %RH). The fabricated sensor was used for the practical applications of health monitoring, touch less sensing, and environment monitoring. Moisture content of multiple commercially available moisturizers was determined by using gelatin based fully biocompatible humidity sensor and the obtained results showed that moisturizing cream had highest moisture content of 87 %RH (0.1 MΩ), followed by 80 %RH (0.30 MΩ) of rose water, and 72 %RH (0.67 MΩ) of baby oil.

IntroductionSingle-port access (SPA) laparoscopy requires only one incision, unlike conventional laparoscopy. However, its umbilical incision is larger than that of conventional laparoscopy and can be vulnerable to postoperative pain. This study aimed to evaluate whether simultaneous use of a continuous wound infiltration (CWI) system and intravenous patient-controlled analgesia (IV PCA) effectively decreases surgical site pain in patients who underwent SPA laparoscopy due to gynecologic adnexal disease.MethodsA total of 371 patients who underwent SPA laparoscopy and who received IV PCA or CWI was retrospectively reviewed (combined group [CWI + IV PCA, n = 159] vs. PCA group [IV PCA only, n = 212]). To evaluate postoperative pain management, the numeric rating scale (NRS) pain score after surgery, total amount of fentanyl administered via IV PCA, and additional pain killer consumption were collected.ResultsThe NRS scores at 12 h (1.90 ± 1.11 vs. 2.70 ± 1.08, p < 0.001) and 24 h (1.82 ± 0.82 vs. 2.11 ± 1.44, p = 0.026) after surgery were significantly lower in the combined group than in the PCA group. The total amount of PCA fentanyl was significantly smaller in the combined group than in the PCA group (p < 0.001). The total quantity of rescue analgesics was smaller in the combined group than in the PCA group (p < 0.05).ConclusionCombined use of the CWI system and IV PCA is an effective postoperative pain management strategy in patient who underwent SPA laparoscopy for adnexal disease.

Employing a combination of Polyethylene terephthalate (PET) thermoforming and 3D-printed cylindrical patterns, we carefully engineer a linear resistive temperature sensor. This intricate process involves initial PET thermoforming, yielding a hollow cylindrical chamber. This chamber is then precisely infused with a composite fluid of graphite and water glue. Ensuring electrical connectivity, both ends are affixed with metal wires and securely sealed using a hot gun. This cost-effective, versatile sensor adeptly gauges temperature shifts by assessing composite fluid resistance alterations. Its PET outer surface grants immunity to water and solubility concerns, enabling application in aquatic and aerial settings without extra encapsulation. Rigorous testing reveals the sensor’s linearity and stability within a 10 °C to 60 °C range, whether submerged or airborne. Beyond 65 °C, plastic deformation arises. To mitigate hysteresis, a 58 °C operational limit is recommended. Examining fluidic composite width and length effects, we ascertain a 12 Ω/°C sensitivity for these linear sensors, a hallmark of their precision. Impressive response and recovery times of 4 and 8 s, respectively, highlight their efficiency. These findings endorse thermoforming’s potential for fabricating advanced temperature sensors. This cost-effective approach’s adaptability underscores its viability for diverse applications.

Photocatalysis is one of the most preferred methods for the degradation of organic pollutants as it can lead to complete mineralization of organic pollutants employing sunlight as an energy source. Until so far enormous number of investigations have been done regarding the development of visible light responsive, stable, and cost effective photocatalysts. In the present work, owing to objectives mentioned above, an attempt was made to develop an efficient, cheaper, and visible light active composite photocatalyst consisting of moderate band gap, CuWO4 with electron conductive graphene quantum dots. A series of photocatalysts was prepared by varying the amount of CuWO4 while keeping the amount of GQDs fixed, and their activity was evaluated for the phenol degradation under visible light irradiation. The prepared photocatalysts were characterized by using X-ray diffraction (XRD), Scanning electron microscopy (SEM), Raman spectroscopy, Fourier Transform Infrared (FTIR) spectroscopy, and Photoluminescence (PL) for the evaluation of crystallinity, functional groups, and properties of charge carrier separation, respectively. The maximum efficiency of photodegradation of phenol simulated wastewater was achieved by sample 0.5GCW (optimized sample having 0.5 wt% of CuWO4 with respect to fixed amount of GQDs), 53.41%, as compared to the pure CuWO4 sample, which exhibited 19.08% efficiency. A possible mechanism for enhanced activity of CuWO4 is proposed mainly due to efficient transfer of electrons from CuWO4 to graphene quantum dots. The results show that the graphene quantum dots GQDs with the CuWO4 significantly contributes to the improvement of photocatalytic performance.

Abstract An experimental study about field emission properties of commercially available graphene flowers cloth is reported. Material characterization by means of X‐ray diffraction, Raman spectroscopy, and X‐ray photoemission spectroscopy confirms the formation of high quality vertical few‐layers graphene nanosheets. A tip‐anode setup is exploited in which nanomanipulated tungsten tip is used as the anode at controlled distance from the emitter in order to reduce the effective emitting area below 1 µm2, giving access to local characterization. A turn‐on field as low as 0.07 V nm−1 and field enhancement factor up to 32 for very small cathode–anode separation distances is demonstrated, in the range 400–700 nm. It is also shown that the turn‐on field increases for increasing distances, while the field enhancement factor decreases. Finally, time stability of the field emission current is reported, evidencing a reduction of the fluctuations for lower current levels.

Biomaterials offer great potential for enhancing the performance of humidity sensors, which play a critical role in controlling moisture levels across different applications. By utilizing environmentally friendly, sustainable, and cost-effective biomaterials, we can improve the manufacturing process of these sensors while reducing our environmental impact. In this study, we present a high-performance humidity sensor that utilizes purple sweet potato peel (PSPP) as both the substrate and sensing layer. The PSPP is chosen for its polar hydrophilic functional groups, as well as its environmentally friendly nature, sustainability, and cost-effectiveness. Remarkably, this humidity sensor does not require an external substrate. It exhibits a wide detection range of 0 to 85% relative humidity at various operating frequencies (100 Hz, 1 kHz, and 10 kHz) in ambient temperature, demonstrating its effectiveness in responding to different humidity levels. The sensor achieves a high sensitivity value of 183.23 pF/%RH and minimal hysteresis of only 5% at 10 kHz under ambient conditions. It also boasts rapid response and recovery times of 1 and 2 s, respectively, making it suitable for use in high-end electronic devices. Moreover, the sensor’s applications extend beyond environmental monitoring. It has proven effective in monitoring mouth and nasal breathing, indicating its potential for respiratory monitoring and noncontact proximity response. These findings suggest that sweet potato peel material holds great promise as a highly stable, non-toxic, biodegradable, cost-effective, and environmentally friendly option for various domains, including healthcare monitoring.