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This study introduces a case of developing and piloting a tailored education program on AI education for beginner-level learners using machine learning platforms such as Google Teachable Machine and Scratch. The program was piloted with 27 elementary school students. Learning effects and satisfaction were examined during the pilot phase. The results suggested that the AI education program had high potential usability, positive learning outcomes, and high satisfaction. Overall, this research paper discusses the development of the AI education program based on relevant literature and the pilot study to evaluate its potential usability in K-12. The study used both quantitative and qualitative responses from the learners.

The Physics is Fun Project is an initiative funded by Purdue's Office of Engagement and aims to promote an interest in physics among young learners aged 5 to 12. The project's unique approach blends instructional design and motivational design to create an engaging and relevant learning experience. Development of the project was guided by the analyze, design, develop, implement, and evaluate (ADDIE) framework and Keller's attention, relevance, confidence, and satisfaction (ARCS) theory and utilized storytelling and hands-on learning strategies to bring physics to life through the story of a young physics enthusiast who is passionate about building a dream car. Basic physics concepts such as force, motion, and energy were presented using age-appropriate language and large infographics with relatable examples. Learners each received a STEM packet to construct their own dream car, therefore providing an authentic learning experience as they assembled their own car with the physics concepts. The first outreach was held in collaboration with Imagination Station in Lafayette and reached over 30 young physics inquirers with minimal scaffolding to support their learning. [ABSTRACT FROM AUTHOR]

Abstract The generation of human oligodendrocyte progenitor cells (OPCs) may be therapeutically valuable for human demyelinating diseases such as multiple sclerosis. Here, we report the direct reprogramming of human somatic cells into expandable induced OPCs (iOPCs) using a combination of OCT4 and a small molecule cocktail. This method enables generation of A2B5+ (an early marker for OPCs) iOPCs within 2 weeks retaining the ability to differentiate into MBP-positive mature oligodendrocytes. RNA-seq analysis revealed that the transcriptome of O4+ iOPCs was similar to that of O4+ OPCs and ChIP-seq analysis revealed that putative OCT4-binding regions were detected in the regulatory elements of CNS development-related genes. Notably, engrafted iOPCs remyelinated the brains of adult shiverer mice and experimental autoimmune encephalomyelitis mice with MOG-induced 14 weeks after transplantation. In conclusion, our study may contribute to the development of therapeutic approaches for neurological disorders, as well as facilitate the understanding of the molecular mechanisms underlying glial development.

Abstract Background Human keratinocytes and derived products are crucial for skin repair and regeneration. Despite substantial advances in engineered skin equivalents, their poor availability and immunorejection remain major challenges in skin grafting. Methods Induced keratinocyte-like cells (iKCs) were directly reprogrammed from human urine cells by retroviral transduction of two lineage-specific transcription factors BMI1 and △NP63α (BN). Expression of keratinocyte stem cell or their differentiation markers were assessed by PCR, immunofluorescence and RNA-Sequencing. Regeneration capacity of iKCs were assessed by reconstitution of a human skin equivalent under air-interface condition. Results BN-driven iKCs were similar to primary keratinocytes (pKCs) in terms of their morphology, protein expression, differentiation potential, and global gene expression. Moreover, BN-iKCs self-assembled to form stratified skin equivalents in vitro. Conclusions This study demonstrated an approach to generate human iKCs that could be directly reprogrammed from human somatic cells and extensively expanded in serum- and feeder cell-free systems, which will facilitate their broad applicability in an efficient and patient-specific manner.

Alopecia: Stimulating hair growth via stem cell-based therapy A treatment developed using stem cells that express high levels of a hair-growth-stimulating protein could help treat alopecia. Cells at the base of hair follicles secrete signaling molecules that help maintain follicle stem cell populations and stimulate hair growth, but defects in these signaling pathways can trigger alopecia. Potential treatments created using stem cells derived from amniotic fluid are being explored by Gwonhwa Song, In Yong Kim and Seungkwon You at the College of Life Sciences and Biotechnology, Seoul, South Korea. They have demonstrated that a protein called Nanog found in amniotic fluid-derived stem cell populations can help maintain stem cell health and stimulate secretion of hair growth proteins. These findings led them to develop a topical treatment with medicines derived from stem cells overexpressing Nanog, which triggered dermal cell proliferation and hair regrowth in mice.

ATP-binding cassette transporter subfamily D member 1 (ABCD1) gene is a member of ABC transporter super family, which conduct peroxisomal import of very long chain fatty acid and crucial underlying factor that induces X-linked adrenoleukodystrophy (X-ALD) when the gene is defected. Here, we report the generation of a human embryonic stem cell sub-line harboring a hemizygous ABCD1 mutation (C.1696_1710 del) using CRISPR/Cas9 system. Established line expresses pluripotency marker genes, can be differentiated to three germ layers, and maintains a normal karyotype.

Charcot-Marie-Tooth disease (CMTX) is inherited neurological disorder caused by gap junction beta 1 gene (GJB1) mutation. We generated induced pluripotent stem cell (iPSC) line from 36-year-old CMTX disease patient by electroporation of skin fibroblasts with episomal vectors encoding OCT4, SOX2, KLF4, L-MYC, LIN28 and shRNA-p53. Established iPSCs expressed various pluripotency markers, had differentiation potential of three germ layers in vitro, had normal karyotype and retained GJB1 mutation. This CMT patient-derived iPSC line could be useful in vitro tool for CMTX research as disease modeling and drug development.

Charcot-Marie-Tooth disease (CMT1B) is an inherited neurological disorder caused by mutation of the myelin protein zero (MPZ) gene. We generated an induced pluripotent stem cell (iPSC) line from an 81-year-old patient with CMT1B by electroporating of lymphoblastoid cell lines with episomal plasmids encoding OCT4, SOX2, KLF4, L-MYC, LIN28, and p53-targeting shRNA. The established iPSCs expressed various pluripotency markers, demonstrated the potential to differentiate into cells of the three germ layers in vitro, had a normal karyotype and retained the MPZ mutation.

Human neural stem cells (NSCs) hold enormous promise for neurological disorders, typically requiring their expandable and differentiable properties for regeneration of damaged neural tissues. Despite the therapeutic potential of induced NSCs (iNSCs), a major challenge for clinical feasibility is the presence of integrated transgenes in the host genome, contributing to the risk for undesired genotoxicity and tumorigenesis. Here, we describe the advanced transgene-free generation of iNSCs from human urine-derived cells (HUCs) by combining a cocktail of defined small molecules with self-replicable mRNA delivery. The established iNSCs were completely transgene-free in their cytosol and genome and further resembled human embryonic stem cell-derived NSCs in the morphology, biological characteristics, global gene expression, and potential to differentiate into functional neurons, astrocytes, and oligodendrocytes. Moreover, iNSC colonies were observed within eight days under optimized conditions, and no teratomas formed in vivo, implying the absence of pluripotent cells. This study proposes an approach to generate transplantable iNSCs that can be broadly applied for neurological disorders in a safe, efficient, and patient-specific manner.