Search

An adeno-associated virus vector encoding an antisense RNA was used to transduce stable intracellular resistance to human immunodeficiency virus-1 (HIV-1) in human hemopoietic and non-hemopoietic cell lines. The antisense targets are present in all HIV-1 transcripts and include the TAR sequence, which is critical for transcription and virus replication, and the polyadenylation signal. Cell lines expressing antisense RNA showed up to 95 percent inhibition of gene expression directed by the HIV-1 long terminal repeat and greater than 99 percent reduction in infectious HIV-1 production, with no detectable cellular toxicity. Because of their efficient transcription and inability to recombine with HIV-1, adeno-associated virus vectors represent a promising form of anti-retroviral gene therapy., Current estimates indicate that more than 1 million people in the United States may be infected with HIV-1, a causative agent of acquired immunodeficiency syndrome (AIDS) [1]. One potential form [...]

Focuses on efforts to develop a vaccine for chronic myelogenous leukemia (CML). Claim that CML has been responsive to immunotherapy and hematopoietic stem-cell transplantation; Outcome of a clinical trial of a BRC-ABL peptide vaccine for CML; Issues impacting the success of CML vaccines; Impact of using imatinib as a treatment for CML.