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Chroma key technique which composes images by separating an object from its background in specific color has restrictions on color and space. Especially, unlike general chroma key technique, image composition for stereo 3D display requires natural image composition method in 3D space. The thesis attempted to compose images in 3D space using depth keying method which uses high resolution depth information. High resolution depth map was obtained through camera calibration between the DSLR and Kinect sensor. 3D mesh model was created by the high resolution depth information and mapped with RGB color value. Object was converted into point cloud type in 3D space after separating it from its background according to depth information. The image in which 3D virtual background and object are composed obtained and played stereo 3D images using a virtual camera.

An unfortunate consequence of improvements in the treatments of advanced primary cancers is the concurrent increase of metastatic brain tumors. Despite of unfavorable clinical prognosis, radiation therapy is still the only viable treatment option for brain metastases. Expression of c-Met induces cell migration and invasion in many cancers, which are indispensable steps for metastasis. Accordingly, we examined the effects of gene silencing of c-Met on brain metastasis to evaluate the possibility of c-Met as a potential target. MDA-MB-435 cells were transfected with c-Met targeting short hairpin RNAs (shRNAs). Effects of c-Met shRNAs on the expression of epithelial mesenchymal transition (EMT) related proteins, in vitro migration, and in vivo brain metastasis were examined. Expression of mesenchymal markers and in vitro migration of MDA-MB-435 cells were significantly inhibited by introduction of c-Met shRNAs. When c-Met-silenced MDA-MB-435 cells were stereotactically implanted into the brains of immune-compromised mice or injected into the right internal carotid arteries, c-Met-silenced MDA-MB-435 cells produced significantly smaller tumor masses or survival time was significantly prolonged, respectively, compared with MDA-MB-435 cells transfected with control shRNA. The data reveal the novel function of c-Met in the process of brain metastasis and its potential as a preventive and/or therapeutic target in this disease.

We prepared a new side-chain polyrotaxane 3 between cyclodextrins (CDs) and grafted poly(p-phenyleneethynylene)s (PPEs) and applied it for the supramolecular functionalization of single-walled carbon nanotubes (SWNTs). Capitalizing on the strong adsorption of the CD's hydroxyl groups on SiOx, we successfully coated silica fibers with SWNTs functionalized by the CD-containing copolymer 3. The significance of the current finding can be extended to other carbon nano-materials, and the functionalization of graphene oxide using the analogs of 3 and its physical adsorption on silica fibers is underway.

Poly(p-phenyleneethynylene)s, composed of a rigid backbone with alternating arylenes and ethynylenes, exhibit high conductivity and chemical stability, and thus they have huge potentials for various applications, including chemosensors, electronic and photonic devices. In spite of numerous interesting applications to date, not many researches concerning oligo(p-phenyleneethynylene)s-graft-poly(ethylene oxide)s (OPEs-g-PEOs) have been carried out, let alone their complexation behaviors with carbon nanomaterials. In this article, we present the synthesis of OPEs-g-PEOs and characterize its morphological and spectral properties by various characterization techniques. We have investigated the interaction of OPEs-g-PEOs with single-walled carbon nanotubes (SWNTs) using various characterization techniques, such as UV-visible, photoluminescence, and Raman spectroscopy, along with SEM, TEM, and POM. In this way, we have successfully demonstrated that OPEs-g-PEOs provide a useful means to modify the physical properties of CNTs, especially in terms of their solubility in common organic solvents.

In this article, we describe a supramolecular complex made of cyclodextrin and a chromophoric dye, in which anistropy occurred by the formation of the hexagonal packing of cylindrical cyclodextrin channels. The obtained complex exhibited effective fluorescence quenching in the presence of lead ions based on supramolecular wire assembly and dissociation. Selective and effective fluorescence quenching of the complex is attributed to the break-ups of the supramolecular wire and the unthreading of included dye molecules by lead ions. Though we are not yet sure about the origin of such a high selectivity, a topic that will be elaborated in subsequent investigations, these CD-templated supramolecular properties will be useful in analytical applications as a novel platform for metal ion sensing.

Background Glioblastoma multiforme (GBM) is characterized by extensive local invasion, which is in contrast with extremely rare systemic metastasis of GBM. Molecular mechanisms inhibiting systemic metastasis of GBM would be a novel therapeutic candidate for GBM in the brain. Methods Patient-derived GBM cells were primarily cultured from surgical samples of GBM patients and were inoculated into the brains of immune deficient BALB/c-nude or NOD-SCID IL2Rgammanull (NSG) mice. Human NK cells were isolated from peripheral blood mononucleated cells and expanded in vitro. Results Patient-derived GBM cells in the brains of NSG mice unexpectedly induced spontaneous lung metastasis although no metastasis was detected in BALB/c-nude mice. Based on the difference of the innate immunity between two mouse strains, NK cell activities of orthotopic GBM xenograft models based on BALB/c-nude mice were inhibited. NK cell inactivation induced spontaneous lung metastasis of GBM cells, which indicated that NK cells inhibit the systemic metastasis. In vitro cytotoxic activities of human NK cells against GBM cells indicated that cytotoxic activity of NK cells against GBM cells prevents systemic metastasis of GBM and that NK cells could be effective cell therapeutics against GBM. Accordingly, NK cells transplanted into orthotopic GBM xenograft models intravenously or intratumorally induced apoptosis of GBM cells in the brain and showed significant therapeutic effects. Conclusions Our results suggest that innate NK immunity is responsible for rare systemic metastasis of GBM and that sufficient supplementation of NK cells could be a promising immunotherapeutic strategy for GBM in the brain. Electronic supplementary material The online version of this article (doi:10.1186/s12885-015-2034-y) contains supplementary material, which is available to authorized users.

In this article, we synthesized an azo dye rotaxane containing bis(8-hydroxyquinoline) group and its polymeric metal complex with zinc. The azo dye rotaxane exhibits high pH sensitivity, solvatochromism and zinc (II) ion sensings in aqueous solution. These behaviors came from the tautomeric equilibrium between azo-hydrazone tautomers and the formation of extended conjugation. The structure of polymeric zinc complexed dye rotaxane was confirmed with NMR and FT-IR measurements. The existence of CD rings, provided by dye rotaxane formation, was found to be very beneficial in improving aqueous solubility of polymeric metal complex.