Search

Your search keyword '"Woehrl S"' showing total 21 results
Start Over Author "Woehrl S"
21 results on '"Woehrl S"'

2. Allergic Rhinitis and its Impact on Asthma (ARIA): Achievements in 10 years and future needs

Author

Bousquet, J., Schünemann, H.J., Samolinski, B., Demoly, P., Baena-Cagnani, C.E., Bachert, C., Bonini, S., Boulet, L.P., Bousquet, P.J., Brozek, J.L., Canonica, G.W., Casale, T.B., Cruz, A.A., Fokkens, W.J., Fonseca, J.A., van Wijk, R. Gerth, Grouse, L., Haahtela, T., Khaltaev, N., Kuna, P., Lockey, R.F., Lodrup Carlsen, K.C., Mullol, J., Naclerio, R., O'Hehir, R.E., Ohta, K., Palkonen, S., Papadopoulos, N.G., Passalacqua, G., Pawankar, R., Price, D., Ryan, D., Simons, F.E.R., Togias, A., Williams, D., Yorgancioglu, A., Yusuf, O.M., Aberer, W., Adachi, M., Agache, I., Aït-Khaled, N., Akdis, C.A., Andrianarisoa, A., Annesi-Maesano, I., Ansotegui, I.J., Baiardini, I., Bateman, E.D., Bedbrook, A., Beghé, B., Beji, M., Bel, E.H., Ben Kheder, A., Bennoor, K.S., Bergmann, K.C., Berrissoul, F., Bieber, T., Bindslev Jensen, C., Blaiss, M.S., Boner, A.L., Bouchard, J., Braido, F., Brightling, C.E., Bush, A., Caballero, F., Calderon, M.A., Calvo, M.A., Camargos, P.A.M., Caraballo, L.R., Carlsen, K.H., Carr, W., Cepeda, A.M., Cesario, A., Chavannes, N.H., Chen, Y.Z., Chiriac, A.M., Chivato Pérez, T., Chkhartishvili, E., Ciprandi, G., Costa, D.J., Cox, L., Custovic, A., Dahl, R., Darsow, U., De Blay, F., Deleanu, D., Denburg, J.A., Devillier, P., Didi, T., Dokic, D., Dolen, W.K., Douagui, H., Dubakiene, R., Durham, S.R., Dykewicz, M.S., El-Gamal, Y., El-Meziane, A., Emuzyte, R., Fiocchi, A., Fletcher, M., Fukuda, T., Gamkrelidze, A., Gereda, J.E., González Diaz, S., Gotua, M., Guzmán, M.A., Hellings, P.W., Hellquist-Dahl, B., Horak, F., Hourihane, J.O'B., Howarth, P., Humbert, M., Ivancevich, J.C., Jackson, C., Just, J., Kalayci, O., Kaliner, M.A., Kalyoncu, A.F., Keil, T., Keith, P.K., Khayat, G., Kim, Y.Y., Koffi N'Goran, B., Koppelman, G.H., Kowalski, M.L., Kull, I., Kvedariene, V., Larenas-Linnemann, D., Le, L.T., Lemière, C., Li, J., Lieberman, P., Lipworth, B., Mahboub, B., Makela, M.J., Martin, F., Marshall, G.D., Martinez, F.D., Masjedi, M.R., Maurer, M., Mavale-Manuel, S., Mazon, A., Melen, E., Meltzer, E.O., Mendez, N.H., Merk, H., Mihaltan, F., Mohammad, Y., Morais-Almeida, M., Muraro, A., Nafti, S., Namazova-Baranova, L., Nekam, K., Neou, A., Niggemann, B., Nizankowska-Mogilnicka, E., Nyembue, T.D., Okamoto, Y., Okubo, K., Orru, M.P., Ouedraogo, S., Ozdemir, C., Panzner, P., Pali-Schöll, I., Park, H.S., Pigearias, B., Pohl, W., Popov, T.A., Postma, D.S., Potter, P., Rabe, K.F., Ratomaharo, J., Reitamo, S., Ring, J., Roberts, R., Rogala, B., Romano, A., Roman Rodriguez, M., Rosado-Pinto, J., Rosenwasser, L., Rottem, M., Sanchez-Borges, M., Scadding, G.K., Schmid-Grendelmeier, P., Sheikh, A., Sisul, J.C., Solé, D., Sooronbaev, T., Spicak, V., Spranger, O., Stein, R.T., Stoloff, S.W., Sunyer, J., Szczeklik, A., Todo-Bom, A., Toskala, E., Tremblay, Y., Valenta, R., Valero, A.L., Valeyre, D., Valiulis, A., Valovirta, E., Van Cauwenberge, P., Vandenplas, O., van Weel, C., Vichyanond, P., Viegi, G., Wang, D.Y., Wickman, M., Wöhrl, S., Wright, J., Yawn, B.P., Yiallouros, P.K., Zar, H.J., Zernotti, M.E., Zhong, N., Zidarn, M., and Zuberbier, T.

Published
2012
Full Text
View/download PDF

3. Practical guide to skin prick tests in allergy to aeroallergens

Author

Bousquet, J., Heinzerling, L., Bachert, C., Papadopoulos, N. G., Bousquet, P. J., Burney, P. G., Canonica, G. W., Carlsen, K. H., Cox, L., Haahtela, T., Lodrup Carlsen, K. C., Price, D., Samolinski, B., Simons, F. E. R., Wickman, M., Annesi-Maesano, I., Baena-Cagnani, C. E., Bergmann, K. C., Bindslev-Jensen, C., Casale, T. B., Chiriac, A., Cruz, A. A., Dubakiene, R., Durham, S. R., Fokkens, W. J., Gerth-van-Wijk, R., Kalayci, O., Kowalski, M. L., Mari, A., Mullol, J., Nazamova-Baranova, L., OʼHehir, R. E., Ohta, K., Panzner, P., Passalacqua, G., Ring, J., Rogala, B., Romano, A., Ryan, D., Schmid-Grendelmeier, P., Todo-Bom, A., Valenta, R., Woehrl, S., Yusuf, O. M., Zuberbier, T., and Demoly, P.

Published
2012
Full Text
View/download PDF

4. Allergen immuntherapy in the current COVID-19 pandemic - A position paper of ARIA, EAACI, AeDA, DGAKI, and GPA

Author

Klimek, L., Pfaar, O., Worm, M., Bergmann, K-C, Bieber, T., Buhl, R., Buters, J., Darsow, U., Keil, T., Kleine-Tebbe, J., Lau, S., Maurer, M., Merk, H., Moesges, R., Saloga, J., Staubach, P., Stute, P., Rabe, K., Rabe, U., Vogelmeier, C., Biedermann, T., Jung, K., Schlenter, W., Ring, J., Chaker, A., Wehrmann, W., Becker, S., Muelleneisen, N., Nemat, K., Czech, W., Wrede, H., Brehler, R., Fuchs, T., Tomazic, P-, V, Aberer, W., Fink-Wagner, A., Horak, F., Woehrl, S., Niederberger-Leppin, V, Pali-Schoell, I, Pohl, W., Roller-Wirnsberger, R., Spranger, O., Valenta, R., Akdis, M., Akdis, C., Hoffmann-Sommergruber, K., Jutel, M., Matricardi, P., Spertini, F., Khaltaev, N., Michel, J-P, Nicod, L., Schmid-Grendelmeier, P., Hamelmann, E., Jakob, T., Werfel, T., Wagenmann, M., Taube, C., Bousquet, J., Zuberbier, T., Vogelberg, C., Gerstlauer, M., Klimek, L., Pfaar, O., Worm, M., Bergmann, K-C, Bieber, T., Buhl, R., Buters, J., Darsow, U., Keil, T., Kleine-Tebbe, J., Lau, S., Maurer, M., Merk, H., Moesges, R., Saloga, J., Staubach, P., Stute, P., Rabe, K., Rabe, U., Vogelmeier, C., Biedermann, T., Jung, K., Schlenter, W., Ring, J., Chaker, A., Wehrmann, W., Becker, S., Muelleneisen, N., Nemat, K., Czech, W., Wrede, H., Brehler, R., Fuchs, T., Tomazic, P-, V, Aberer, W., Fink-Wagner, A., Horak, F., Woehrl, S., Niederberger-Leppin, V, Pali-Schoell, I, Pohl, W., Roller-Wirnsberger, R., Spranger, O., Valenta, R., Akdis, M., Akdis, C., Hoffmann-Sommergruber, K., Jutel, M., Matricardi, P., Spertini, F., Khaltaev, N., Michel, J-P, Nicod, L., Schmid-Grendelmeier, P., Hamelmann, E., Jakob, T., Werfel, T., Wagenmann, M., Taube, C., Bousquet, J., Zuberbier, T., Vogelberg, C., and Gerstlauer, M.

Published
2020

5. Use of biologicals in allergic and type 2 inflammatory diseases in the current COVID 19 pandemic

Author

Klimek, L., Pfaar, O., Worm, M., Eiwegger, T., Hagemann, J., Ollert, M., Untersmayr, E., Hoffmann-Sommergruber, K., Vultaggio, A., Agache, I, Bavbek, S., Bossios, A., Casper, I, Chan, S., Chatzipetrou, A., Vogelberg, C., Firinu, D., Kauppi, P., Kolios, A., Kothari, A., Matucci, A., Palomares, O., Szepfalusi, Z., Pohl, W., Hoetzenecker, W., Rosenkranz, A., Bergmann, K-C, Bieber, T., Buhl, R., Buters, J., Darsow, U., Keil, T., Kleine-Tebbe, J., Lau, S., Maurer, M., Merk, H., Moesges, R., Saloga, J., Staubach, P., Jappe, U., Rabe, C., Rabe, U., Vogelmeier, C., Biedermann, T., Jung, K., Schlenter, W., Ring, J., Chaker, A., Wehrmann, W., Becker, S., Freudelsperger, L., Muelleneisen, N., Nemat, K., Czech, W., Wrede, H., Brehler, R., Fuchs, T., Tomazic, P-, V, Aberer, W., Fink-Wagner, A., Horak, F., Woehrl, S., Niederberger-Leppin, V, Pali-Schoell, I, Roller-Wirnsberger, R., Spranger, O., Valenta, R., Akdis, M., Matricardi, P. M., Spertini, F., Khaltaev, N., Michel, J-P, Nicod, L., Schmid-Grendelmeier, P., Idzko, M., Hamelmann, E., Jakob, T., Werfel, T., Wagenmann, M., Taube, C., Jensen-Jarolim, E., Korn, S., Hentges, F., Schwarze, J., OMahony, L., Knol, E., del Giacco, S., Chivato Perez, T., Bousquet, J., Zuberbier, T., Akdis, C., Jutel, M., Klimek, L., Pfaar, O., Worm, M., Eiwegger, T., Hagemann, J., Ollert, M., Untersmayr, E., Hoffmann-Sommergruber, K., Vultaggio, A., Agache, I, Bavbek, S., Bossios, A., Casper, I, Chan, S., Chatzipetrou, A., Vogelberg, C., Firinu, D., Kauppi, P., Kolios, A., Kothari, A., Matucci, A., Palomares, O., Szepfalusi, Z., Pohl, W., Hoetzenecker, W., Rosenkranz, A., Bergmann, K-C, Bieber, T., Buhl, R., Buters, J., Darsow, U., Keil, T., Kleine-Tebbe, J., Lau, S., Maurer, M., Merk, H., Moesges, R., Saloga, J., Staubach, P., Jappe, U., Rabe, C., Rabe, U., Vogelmeier, C., Biedermann, T., Jung, K., Schlenter, W., Ring, J., Chaker, A., Wehrmann, W., Becker, S., Freudelsperger, L., Muelleneisen, N., Nemat, K., Czech, W., Wrede, H., Brehler, R., Fuchs, T., Tomazic, P-, V, Aberer, W., Fink-Wagner, A., Horak, F., Woehrl, S., Niederberger-Leppin, V, Pali-Schoell, I, Roller-Wirnsberger, R., Spranger, O., Valenta, R., Akdis, M., Matricardi, P. M., Spertini, F., Khaltaev, N., Michel, J-P, Nicod, L., Schmid-Grendelmeier, P., Idzko, M., Hamelmann, E., Jakob, T., Werfel, T., Wagenmann, M., Taube, C., Jensen-Jarolim, E., Korn, S., Hentges, F., Schwarze, J., OMahony, L., Knol, E., del Giacco, S., Chivato Perez, T., Bousquet, J., Zuberbier, T., Akdis, C., and Jutel, M.

Published
2020

6. Handling of allergen immunotherapy in the COVID-19 pandemic: An ARIA- EAACI-AeDA-GPA-DGAKI Position Paper (Pocket-Guide)

Author

Pfaar, O., Klimek, L., Worm, M., Bergmann, K-C, Bieber, T., Buhl, R., Buters, J., Darsow, U., Keil, T., Kleine-Tebbe, J., Lau, S., Maurer, M., Merk, H., Mosges, R., Saloga, J., Staubach, P., Stute, P., Rabe, K., Rabe, U., Vogelmeier, C., Biedermann, T., Jung, K., Schlenter, W., Ring, J., Chaker, A., Wehrmann, W., Becker, S., Muelleneisen, N., Nemat, K., Czech, W., Wrede, H., Brehler, R., Fuchs, T., Tomazic, P-, V, Aberer, W., Fink-Wagner, A., Horak, F., Woehrl, S., Niederberger-Leppin, V, Pali-Schoell, I, Pohl, W., Roller-Wirnsberger, R., Spranger, O., Valenta, R., Akdis, M., Akdis, C., Hoffmann-Sommergruber, K., Jutel, M., Matricardi, P., Spertini, F., Khaltaev, N., Michel, J-P, Nicod, L., Schmid-Grendelmeier, P., Hamelmann, E., Jakob, T., Werfel, T., Wagenmann, M., Taube, C., Gerstlauer, M., Vogelberg, C., Bousquet, J., Zuberbier, T., Pfaar, O., Klimek, L., Worm, M., Bergmann, K-C, Bieber, T., Buhl, R., Buters, J., Darsow, U., Keil, T., Kleine-Tebbe, J., Lau, S., Maurer, M., Merk, H., Mosges, R., Saloga, J., Staubach, P., Stute, P., Rabe, K., Rabe, U., Vogelmeier, C., Biedermann, T., Jung, K., Schlenter, W., Ring, J., Chaker, A., Wehrmann, W., Becker, S., Muelleneisen, N., Nemat, K., Czech, W., Wrede, H., Brehler, R., Fuchs, T., Tomazic, P-, V, Aberer, W., Fink-Wagner, A., Horak, F., Woehrl, S., Niederberger-Leppin, V, Pali-Schoell, I, Pohl, W., Roller-Wirnsberger, R., Spranger, O., Valenta, R., Akdis, M., Akdis, C., Hoffmann-Sommergruber, K., Jutel, M., Matricardi, P., Spertini, F., Khaltaev, N., Michel, J-P, Nicod, L., Schmid-Grendelmeier, P., Hamelmann, E., Jakob, T., Werfel, T., Wagenmann, M., Taube, C., Gerstlauer, M., Vogelberg, C., Bousquet, J., and Zuberbier, T.

Published
2020

7. Practical guide to skin prick tests in allergy to aeroallergens

Author

Bousquet, J, Heinzerling, L, Bachert, C, Papadopoulos, NG, Bousquet, PJ, Burney, PG, Canonica, GW, Carlsen, KH, Cox, L, Haahtela, T, Carlsen, KCL, Price, D, Samolinski, B, Simons, FER, Wickman, M, Annesi-Maesano, I, Baena-Cagnani, CE, Bergmann, KC, Bindslev-Jensen, C, Casale, TB, Chiriac, A, Cruz, AA, Dubakiene, R, Durham, SR, Fokkens, WJ, Gerth van Wijk, Roy, Kalayci, O, Kowalski, ML, Mari, A, Mullol, J, Nazamova-Baranova, L, O'Hehir, RE, Ohta, K, Panzner, P, Passalacqua, G, Ring, J, Rogala, B, Romano, A, Ryan, D, Schmid-Grendelmeier, P, Todo-Bom, A, Valenta, R, Woehrl, S, Yusuf, OM, Zuberbier, T, Demoly, P, Epidémiologie des maladies infectieuses et modélisation (ESIM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Dermatology, Medical School-Charité - UniversitätsMedizin = Charité - University Hospital [Berlin], Bousquet J, Heinzerling L, Bindslev-Jensen C, Casale TB, Bachert C, Chiriac A, Cruz AA, Dubakiene R, Durham SR, Fokkens WJ, Gerth-van-Wijk R, Kalayci O, Lodrup Carlsen KC, Kowalski ML, Mari A, Mullol J, Papadopoulos NG, Nazamova-Baranova L, O'Hehir RE, Ohta K, Panzner P, Passalacqua G, Ring J, Price D, Rogala B, Romano A, Ryan D, Schmid-Grendelmeier P, Bousquet PJ, Todo-Bom A, Valenta R, Woehrl S, Yusuf OM, Zuberbier T, Samolinski B, Demoly P, Burney PG, Canonica GW, Carlsen KH, Cox L, Haahtela T, Simons FE, Wickman M, Annesi-Maesano I, Baena-Cagnani CE, Bergmann KC, AII - Amsterdam institute for Infection and Immunity, Ear, Nose and Throat, University of Zurich, Bousquet, J, Dermatology, and Internal Medicine

Subjects
2403 Immunology, Air Pollutants, 2723 Immunology and Allergy, Hypersensitivity, 10177 Dermatology Clinic, Humans, 610 Medicine & health, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Allergens, Skin Tests
Abstract

International audience; To cite this article: Bousquet J, Heinzerling L, Bachert C, Papadopoulos NG, Bousquet PJ, Burney PG, Canonica GW, Carlsen KH, Cox L, Haahtela T, Lodrup Carlsen KC, Price D, Samolinski B, Simons FER, Wickman M, Annesi-Maesano I, Baena-Cagnani CE, Bergmann KC, Bindslev-Jensen C, Casale TB, Chiriac A, Cruz AA, Dubakiene R, Durham SR, Fokkens WJ, Gerth-van-Wijk R, Kalayci O, Kowalski ML, Mari A, Mullol J, Nazamova-Baranova L, O'Hehir RE, Ohta K, Panzner P, Passalacqua G, Ring J, Rogala B, Romano A, Ryan D, Schmid-Grendelmeier P, Todo-Bom A, Valenta R, Woehrl S, Yusuf OM, Zuberbier T, Demoly P. Practical guide to skin prick tests in allergy to aeroallergens. Allergy 2011; DOI: 10.1111/j.1398-9995.2011.02728.x ABSTRACT: This pocket guide is the result of a consensus reached between members of the Global Allergy and Asthma European Network (GA(2) LEN) and Allergic Rhinitis and its Impact on Asthma (ARIA). The aim of the current pocket guide is to offer a comprehensive set of recommendations on the use of skin prick tests in allergic rhinitis-conjunctivitis and asthma in daily practice. This pocket guide is meant to give simple answers to the most frequent questions raised by practitioners in Europe, including 'practicing allergists', general practitioners and any other physicians with special interest in the management of allergic diseases. It is not a long or detailed scientific review of the topic. However, the recommendations in this pocket guide were compiled following an in-depth review of existing guidelines and publications, including the 1993 European Academy of Allergy and Clinical Immunology position paper, the 2001 ARIA document and the ARIA update 2008 (prepared in collaboration with GA(2) LEN). The recommendations cover skin test methodology and interpretation, allergen extracts to be used, as well as indications in a variety of settings including paediatrics and developing countries.

Published
2011
Full Text
View/download PDF

8. Practical guide to skin prick tests in allergy to aeroallergens

Author

Bousquet, J. Heinzerling, L. Bachert, C. Papadopoulos, N.G. Bousquet, P.J. Burney, P.G. Canonica, G.W. Carlsen, K.H. Cox, L. Haahtela, T. Lodrup Carlsen, K.C. Price, D. Samolinski, B. Simons, F.E.R. Wickman, M. Annesi-Maesano, I. Baena-Cagnani, C.E. Bergmann, K.C. Bindslev-Jensen, C. Casale, T.B. Chiriac, A. Cruz, A.A. Dubakiene, R. Durham, S.R. Fokkens, W.J. Gerth-Van-Wijk, R. Kalayci, O. Kowalski, M.L. Mari, A. Mullol, J. Nazamova-Baranova, L. O'Hehir, R.E. Ohta, K. Panzner, P. Passalacqua, G. Ring, J. Rogala, B. Romano, A. Ryan, D. Schmid-Grendelmeier, P. Todo-Bom, A. Valenta, R. Woehrl, S. Yusuf, O.M. Zuberbier, T. Demoly, P.

Abstract

This pocket guide is the result of a consensus reached between members of the Global Allergy and Asthma European Network (GA 2LEN) and Allergic Rhinitis and its Impact on Asthma (ARIA). The aim of the current pocket guide is to offer a comprehensive set of recommendations on the use of skin prick tests in allergic rhinitis-conjunctivitis and asthma in daily practice. This pocket guide is meant to give simple answers to the most frequent questions raised by practitioners in Europe, including 'practicing allergists', general practitioners and any other physicians with special interest in the management of allergic diseases. It is not a long or detailed scientific review of the topic. However, the recommendations in this pocket guide were compiled following an in-depth review of existing guidelines and publications, including the 1993 European Academy of Allergy and Clinical Immunology position paper, the 2001 ARIA document and the ARIA update 2008 (prepared in collaboration with GA 2LEN). The recommendations cover skin test methodology and interpretation, allergen extracts to be used, as well as indications in a variety of settings including paediatrics and developing countries. © 2011 John Wiley & Sons A/S.

Published
2012

9. Allergy

Author

Bousquet, Jean, Heinzerling, L., Bachert, Claus, Papadopoulos, N. G., Bousquet, P. J., Burney, P. G., Canonica, Giorgio W., Carlsen, K. H., Cox, L., Haahtela, T., Carlsen, Lodrup K. C., Prince, D., Samolinski, B., Simons, F. E. R., Wickman, M., Annesi-Maesano, I., Baena-Cagnani, C. E., Bergmann, K. C., Bindslev-Jensen, J., Casale, T. B., Chiriac, A., Cruz Filho, Álvaro Augusto Souza da, Chiriac, R., Chiriac, S. R., Chiriac, W. J., Gerth-van-Wijk, R., Kalayci, Ö., Kowalski, M. L., Mari, A., Mullol, J., Nazamova-Baranova, L., O'Hehir, R. E., Ohta, K., Panzner, P., Passalacqua, G., Ring, J., Rogala, B., Romano, A., Ryan, D., Schmid-Grendelmeier, P., Todo-Bom, A., Valenta, A., Woehrl, S., Yusuf, O. M., Zuberbier, Torsten, and Demoly, P.

Subjects
Allergy, Skin prick test, Inhalant, Asthma, Rhinitis
Abstract

Texto completo: acesso restrito. p. 18–24 Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-08-27T14:59:42Z No. of bitstreams: 1 A. A. Cruz.pdf: 209888 bytes, checksum: 7d347fe02cf77a0c8ede71e0efb225bf (MD5) Approved for entry into archive by Delba Rosa (delba@ufba.br) on 2014-09-23T14:48:34Z (GMT) No. of bitstreams: 1 A. A. Cruz.pdf: 209888 bytes, checksum: 7d347fe02cf77a0c8ede71e0efb225bf (MD5) Made available in DSpace on 2014-09-23T14:48:35Z (GMT). No. of bitstreams: 1 A. A. Cruz.pdf: 209888 bytes, checksum: 7d347fe02cf77a0c8ede71e0efb225bf (MD5) Previous issue date: 2012 This pocket guide is the result of a consensus reached between members of the Global Allergy and Asthma European Network (GA2LEN) and Allergic Rhinitis and its Impact on Asthma (ARIA). The aim of the current pocket guide is to offer a comprehensive set of recommendations on the use of skin prick tests in allergic rhinitis–conjunctivitis and asthma in daily practice. This pocket guide is meant to give simple answers to the most frequent questions raised by practitioners in Europe, including ‘practicing allergists’, general practitioners and any other physicians with special interest in the management of allergic diseases. It is not a long or detailed scientific review of the topic. However, the recommendations in this pocket guide were compiled following an in-depth review of existing guidelines and publications, including the 1993 European Academy of Allergy and Clinical Immunology position paper, the 2001 ARIA document and the ARIA update 2008 (prepared in collaboration with GA2LEN). The recommendations cover skin test methodology and interpretation, allergen extracts to be used, as well as indications in a variety of settings including paediatrics and developing countries.

Published
2012
Full Text
View/download PDF

10. TNF-like weak inducer of apoptosis (TWEAK) and TNF-α cooperate in the induction of keratinocyte apoptosis

Author

Zimmermann, M, Koreck, A, Meyer, N, Basinski, T, Meiler, F, Simone, B, Woehrl, S, Moritz, K, Eiwegger, T, Schmid-Grendelmeier, P, Kemeny, L, Akdis, C A, University of Zurich, and Zimmermann, M

Subjects
2403 Immunology, 10183 Swiss Institute of Allergy and Asthma Research, 2723 Immunology and Allergy, 10177 Dermatology Clinic, 610 Medicine & health
Published
2011
Full Text
View/download PDF

11. TNF-like weak inducer of apoptosis (TWEAK) and TNF-alpha cooperate in the induction of keratinocyte apoptosis

Author

Zimmermann M, Koreck A, Meyer N, Basinski T, Meiler F, Simone B, Woehrl S, Moritz K, Eiwegger T, Schmid-Grendelmeier P, Kemeny L, and Akdis CA

Abstract

Background: Activation of skin keratinocytes followed by their apoptotic death leads to eczema and spongiosis formations in patients with atopic dermatitis (AD). TNF like weak inducer of apoptosis (TWEAK) binds to its receptor fibroblast growth factor inducible 14 (Fn14) and controls many cellular activities including proliferation migration differentiation apoptosis angiogenesis and inflammation. Objective: The aim of the study was to investigate the role of TWEAK and Fn14 in the formation of eczema in patients with AD. Methods: Primary keratinocytes were isolated from nonlesional skin from patients with AD and psoriasis and from normal skin of healthy donors. Apoptosis analysis was performed by using annexin V/7 aminoactinomycin D and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling staining. The expression and regulation of TWEAK TNF alpha Fn14 TNF receptor (TNFR) 1 and TNFR2 were measured by means of RT PCR flow cytometric analysis and ELISA. TWEAK and Fn14 expression of lesional AD and psoriatic skin and normal control skin was analyzed by using immunohistochemistry and immunofluorescence. Results: TWEAK and TNF alpha cooperate in the induction of apoptosis in primary keratinocytes obtained from patients with AD patients with psoriasis and healthy subjects and in artificial skin equivalents. TNFR1 and Fn14 were the main receptors involved. TWEAK upregulates TNF alpha expression in primary keratinocytes whereas TNF alpha did not affect the expression of TWEAK and its receptors. High TWEAK expression was observed in AD lesions but not in psoriatic lesions or normal skin. Fn14 was highly expressed in the lesional skin of patients with AD and patients with psoriasis and in healthy control skin. Conclusion: The high expression of TWEAK in lesional AD skin contributes to the difference in keratinocyte apoptosis and lesional formation between AD and psoriasis. (J Allergy Clin Immunol 2011;127:200 7.)

Published
2012

20. TNF-like weak inducer of apoptosis (TWEAK) and TNF-α cooperate in the induction of keratinocyte apoptosis.

Author

Zimmermann M, Koreck A, Meyer N, Basinski T, Meiler F, Simone B, Woehrl S, Moritz K, Eiwegger T, Schmid-Grendelmeier P, Kemeny L, and Akdis CA

Subjects
Cell Separation, Cells, Cultured, Cytokine TWEAK, Dermatitis, Atopic complications, Dermatitis, Atopic metabolism, Dermatitis, Atopic pathology, Eczema etiology, Eczema pathology, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Fluorescent Antibody Technique, Humans, Immunohistochemistry, In Situ Nick-End Labeling, Keratinocytes pathology, Reverse Transcriptase Polymerase Chain Reaction, Apoptosis physiology, Eczema metabolism, Keratinocytes metabolism, Tumor Necrosis Factor-alpha metabolism, Tumor Necrosis Factors metabolism
Abstract

Background: Activation of skin keratinocytes followed by their apoptotic death leads to eczema and spongiosis formations in patients with atopic dermatitis (AD). TNF-like weak inducer of apoptosis (TWEAK) binds to its receptor, fibroblast growth factor-inducible 14 (Fn14), and controls many cellular activities, including proliferation, migration, differentiation, apoptosis, angiogenesis, and inflammation., Objective: The aim of the study was to investigate the role of TWEAK and Fn14 in the formation of eczema in patients with AD., Methods: Primary keratinocytes were isolated from nonlesional skin from patients with AD and psoriasis and from normal skin of healthy donors. Apoptosis analysis was performed by using annexin V/7-aminoactinomycin D and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling staining. The expression and regulation of TWEAK, TNF-α, Fn14, TNF receptor (TNFR) 1, and TNFR2 were measured by means of RT-PCR, flow cytometric analysis, and ELISA. TWEAK and Fn14 expression of lesional AD and psoriatic skin and normal control skin was analyzed by using immunohistochemistry and immunofluorescence., Results: TWEAK and TNF-α cooperate in the induction of apoptosis in primary keratinocytes obtained from patients with AD, patients with psoriasis, and healthy subjects and in artificial skin equivalents. TNFR1 and Fn14 were the main receptors involved. TWEAK upregulates TNF-α expression in primary keratinocytes, whereas TNF-α did not affect the expression of TWEAK and its receptors. High TWEAK expression was observed in AD lesions but not in psoriatic lesions or normal skin. Fn14 was highly expressed in the lesional skin of patients with AD and patients with psoriasis and in healthy control skin., Conclusion: The high expression of TWEAK in lesional AD skin contributes to the difference in keratinocyte apoptosis and lesional formation between AD and psoriasis., (Copyright © 2010 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.)

Published
2011
Full Text
View/download PDF

21. IL-32 is expressed by human primary keratinocytes and modulates keratinocyte apoptosis in atopic dermatitis.

Author

Meyer N, Zimmermann M, Bürgler S, Bassin C, Woehrl S, Moritz K, Rhyner C, Indermitte P, Schmid-Grendelmeier P, Akdis M, Menz G, and Akdis CA

Subjects
Cells, Cultured, Humans, Interleukins pharmacology, Keratinocytes immunology, Keratinocytes physiology, T-Lymphocytes immunology, Apoptosis drug effects, Dermatitis, Atopic immunology, Dermatitis, Atopic physiopathology, Interleukins metabolism, Keratinocytes metabolism
Abstract

Background: Keratinocyte (KC) apoptosis is an important mechanism of eczema and spongiosis in patients with atopic dermatitis (AD) and is mediated by IFN-gamma, which is secreted by T(H)1 cells. IL-32 is a proinflammatory cytokine that is involved in the inflammatory processes of rheumatoid arthritis, chronic obstructive pulmonary disease, and Crohn disease. Recently, it was shown that upregulation of IL-32 induces apoptosis., Objective: The aim of the study was to investigate the expression and function of IL-32 in patients with AD., Methods: The expression of IL-32 in KCs was analyzed by means of RT-PCR, ELISA, and flow cytometry. Transfections of small interfering RNA were performed in primary KCs, and apoptosis was analyzed by means of terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling, annexin-V, and 7-amino actinomycin D stainings. Immunofluorescence stainings were used to detect IL-32 in skin biopsy specimens, and serum levels of IL-32 were analyzed by means of ELISA., Results: We report that IL-32 is expressed in human primary KCs on stimulation with IFN-gamma, TNF-alpha, and T(H)1 cells in contrast to T(H)2, regulatory T (Treg), or T(H)17 cells, which showed no effect. Transfection of primary KCs and artificial skin equivalents with small interfering RNA to IL-32, which resulted in a clear decrease in IL-32 expression, significantly reduced KC apoptosis. Immunofluorescence staining demonstrated that IL-32 was expressed in AD lesional skin, whereas it was present in neither skin biopsy specimens from healthy donors nor in lesional skin from patients with psoriasis. Serum levels of IL-32 from patients with AD correlated with disease severity, but increased serum levels of IL-32 were also detected in asthmatic patients., Conclusion: The present study demonstrates KCs as a source of IL-32, which modulates KC apoptosis and contributes to the pathophysiology of AD., (Copyright (c) 2010 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.)

Published
2010
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results