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5 results on '"Wiwat Pattanawong"'

1. The Use of Essential Oil-based Pharmaceutical Products to Control Cattle Ticks

Author

Apichart Manwicha, Wiwat Pattanawong, Nattha Vigad, Sunee Chansakaow, Pramote Tipduangta, and Kridda Chukiatsiri

Subjects
Veterinary medicine, SF600-1100
Abstract

The purpose of this study was to compare the efficacy of six essential oils obtained from sour orange (Citrus aurantium L.), ginger (Zingiber officinale Roscoe), lemongrass (Cymbopogon citratus (DC.) Stapf.), litsea (Litsea cubeba (Lour.) Pers.), clove (Eugenia caryophyllata Thunb.) and curcumin (Curcuma longa Linn.) against cattle ticks. Two essential oils demonstrated the greatest significant efficiency and were chosen for further testing on the cattle skin. The results showed that the 8% lemongrass essential oil had the most significant inhibiting effect on the oviposition of engorged female cattle ticks, whereas ginger oil, curcumin oil, sour orange, litsea oil and clove oil, showed lesser effect respectively (p

Published
2024
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2. High-Efficiency Bovine Sperm Sexing Used Magnetic-Activated Cell Sorting by Coupling scFv Antibodies Specific to Y-Chromosome-Bearing Sperm on Magnetic Microbeads

Author

Korawan Sringarm, Marninphan Thongkham, Supamit Mekchay, Chompunut Lumsangkul, Wannaluk Thaworn, Wiwat Pattanawong, Ekaphot Rangabpit, Pornchai Rachtanapun, Kittisak Jantanasakulwong, Anucha Sathanawongs, and Surat Hongsibsong

Subjects
bull semen, sexing semen, magnetic-activated cell sorting, scFv antibody, semen quality, Biology (General), QH301-705.5
Abstract

Sperm sexing technique is favored in the dairy industry. This research focuses on the efficiency of bovine sperm sexing using magnetic-activated cell sorting (MACS) by scFv antibody against Y-chromosome-bearing sperm (Y-scFv) coupled to magnetic microbeads and its effects on kinematic variables, sperm quality, and X/Y-sperm ratio. In this study, the optimal concentration of Y-scFv antibody coupling to the surface of magnetic microbeads was 2–4 mg/mL. PY-microbeads revealed significantly enriched Y-chromosome-bearing sperm (Y-sperm) in the eluted fraction (78.01–81.43%) and X-chromosome-bearing sperm (X-sperm) in the supernatant fraction (79.04–82.65%). The quality of frozen–thawed sexed sperm was analyzed by CASA and imaging flow cytometer, which showed that PY-microbeads did not have a negative effect on X-sperm motility, viability, or acrosome integrity. However, sexed Y-sperm had significantly decreased motility and viability. The X/Y-sperm ratio was determined using an imaging flow cytometer and real-time PCR. PY-microbeads produced sperm with up to 82.65% X-sperm in the X-enriched fraction and up to 81.43% Y-sperm in the Y-enriched fraction. Bovine sperm sexing by PY-microbeads showed high efficiency in separating Y-sperm from X-sperm and acceptable sperm quality. This initial technique is feasible for bovine sperm sexing, which increases the number of heifers in dairy herds while lowering production expenses.

Published
2022
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3. Production of single-chain fragment variable (scFv) antibodies specific to plasma membrane epitopes on bull Y-bearing sperm

Author

Wiwat Pattanawong, Surat Hongsibsong, Korawan Sringarm, Supamit Mekchay, Wannaluk Thaworn, and Marninphan Thongkham

Subjects
Male, endocrine system, animal diseases, Bioengineering, Single chain, Biology, Epitope, Epitopes, fluids and secretions, Animals, ScFv Antibodies, Cloning, Molecular, reproductive and urinary physiology, Hybridomas, urogenital system, Fragment (computer graphics), Cell Membrane, Antibodies, Monoclonal, Bull semen, Spermatozoa, Sperm, Molecular biology, Membrane, Cattle, Animal Science and Zoology, Single-Chain Antibodies, Biotechnology
Abstract

Distinguishing between bull Y- and X-bearing sperm populations is advantageous for techniques using sexed bull semen. The aim of this study was to produce a single-chain fragment variable (scFv) antibody against plasma membrane epitopes on bull Y-bearing sperm. Variable heavy (VH)- and variable light (VL)-region genes generated from a hybridoma cell secreting a specific Y-bearing sperm monoclonal antibody (mAb-1F9) were cloned and expressed. The expected sizes of the DNA bands were ∼350 bp for the VH gene and ∼318 bp for the VL gene. The VH and VL genes were generated and used to construct an scFv gene (∼650 bp), which was expressed in

Published
2020

4. Spermatological parameters of immunologically sexed bull semen assessed by imaging flow cytometry, and dairy farm trial

Author

Korawan Sringarm, Marninphan Thongkham, Wiwat Pattanawong, Sorn Teepatimakorn, Supamit Mekchay, and Wannaluk Thaworn

Subjects
0301 basic medicine, Imaging flow cytometry, Male, endocrine system, X Chromosome, Pregnancy Rate, Cell Survival, Semen, Sexing, Biology, urologic and male genital diseases, Andrology, 03 medical and health sciences, Semen quality, fluids and secretions, 0302 clinical medicine, Endocrinology, Pregnancy, Y Chromosome, Freezing, Image Processing, Computer-Assisted, Animals, Sex Preselection, Acrosome, Cryopreservation, 030219 obstetrics & reproductive medicine, urogenital system, Acrosome Reaction, Antibodies, Monoclonal, Flow Cytometry, Sperm, Pregnancy rate, 030104 developmental biology, Herd, Sperm Motility, Animal Science and Zoology, Cattle, Female, Developmental Biology, Semen Preservation
Abstract

This study compared the quality parameters of bull semen sexed using an immunological method with those of conventional semen by imaging flow cytometry and applied this semen in dairy farm trials. Semen samples were collected from ten ejaculates from five bulls. Each sample was divided into two treatments: conventional semen (CON) and semen sexed using monoclonal male-specific antibodies combined with the complement system for cytotoxicity reaction (IC-sexed). After obtaining frozen-thawed semen, we used imaging flow cytometry to assess acrosome integrity, sperm sex ratio and viability. Sperm morphology was evaluated using eosin-nigrosin staining. The percentage acrosome integrity did not differ between IC-sexed and CON semen (P = 0.313). The sperm sex ratio showed that the percentage of live X-chromosome-bearing sperm was higher than that of live Y-chromosome-bearing sperm in IC-sexed semen (P = 0.001). IC-sexed semen showed a higher percentage of head and tail defects than did CON semen (P = 0.019). In field trials, 585 cows were subjected randomly to AI with CON or IC-sexed semen. The pregnancy rate of the IC-sexed group did not differ from that of the CON group (P = 0.535). However, IC-sexed semen produced a significantly higher percentage of female calves than did CON semen (P = 0.031). Thus, immunological sexing did not adversely affect the acrosome integrity of sperm. Furthermore, a female calf birth rate of over 74 % can potentially be achieved using IC-sexed semen. These findings could help farmers to replace heifers in their herds.

Published
2020

5. Carcass Characteristics and Meat Quality of Thai Inheritance Chickens

Author

Nakarin Pripwai, Tawatchai Teltathum, Montri Punyatong, and Wiwat Pattanawong

Subjects
animal structures, Carcass weight, Animal science, embryonic structures, food and beverages, Water holding capacity, Biology
Abstract

Inheritance chickens are important in the developing countries, because of their meat quality. The present study was performed to investigate the effect of genetic background of Baetong, Black-boned, and Praduhangdum chickens. 30 chickens each and 4 replications of the chicken breeds were reared for 14 weeks. 10 chickens of each replication were sampled and analyzed, including carcass characteristics and meat quality as well as Shear’s value, water holding capacity, and color of their meat. No different marketable weight and hot carcass weight was found (P>0.05). Baetong chickens were lower hot carcass yield than Black-boned chickens and Praduhangdum chickens (P

Published
2014

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