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10 results on '"Wittschier N"'

1. An extract of Pelargonium sidoides (EPs 7630) inhibits in situ adhesion of Helicobacter pylori to human stomach

Author

Wittschier, N., Faller, G., and Hensel, A.

Subjects
Medical research -- Analysis, Medicine, Experimental -- Analysis, Helicobacter pylori -- Analysis -- Research, Geraniums -- Research -- Dosage and administration -- Analysis, Biological sciences, Health, Science and technology
Abstract

Abstract Root extract from Pelargonium sidoides DC is used therapeutically as antimicrobial agent against infections of the respiratory system. In order to elucidate possible modes of actions we investigated the [...]

Published
2007

3. Occurrence of N-Phenylpropenoyl-<scp>L</scp>-amino Acid Amides in Different Herbal Drugs and their Influence on Human Keratinocytes, on Human Liver Cells and on Adhesion of Helicobacter pylori to the Human Stomach

Author

Andreas Hensel, Timo D. Stark, Thomas Hofmann, G. Müller, Wittschier. N., and Alexandra Deters

Subjects
Keratinocytes, Coriandrum, Angelica archangelica, Pharmaceutical Science, Cassia angustifolia, Sambucus nigra, Isozyme, Bacterial Adhesion, Mass Spectrometry, Analytical Chemistry, Drug Discovery, Gene expression, Humans, Amino Acids, Cell Proliferation, Pharmacology, chemistry.chemical_classification, Plants, Medicinal, Helicobacter pylori, biology, Stomach, Organic Chemistry, biology.organism_classification, Amides, Amino acid, Enzyme, Gene Expression Regulation, Complementary and alternative medicine, chemistry, Biochemistry, Hepatocytes, Molecular Medicine, STAT6 Transcription Factor, Chromatography, Liquid
Abstract

Thirty commonly used medicinal plants were screened by a selective and specific LC-MS/MS method for the occurrence of N-phenylpropenoyl- L-amino acid amides, a new homologous class of secondary products. In 15 plants, one or more of the respective derivatives (1 to 12) were found and quantitated. Especially roots from Angelica archangelica, fruits of Cassia angustifolia, C. senna, Coriandrum sativum, leaves from Hedera helix, flowers from Lavandula spec. and from Sambucus nigra contained high amounts (1 to 11 microg/g) of mixtures of the different amides 1 to 12. For functional investigations on potential activity in cellular physiology, two amides with an aliphatic (8) and an aromatic amino acid residue (5) were used. N-(E)-Caffeic acid L-aspartic acid amide (8) and N-(E)-caffeic acid L-tryptophan amide (5) stimulated mitochondrial activity as well as the proliferation rate of human liver cells (HepG2) at 10 microg/mL significantly. When monitoring the influence of selected phase I and II metabolizing enzymes, both compounds did not influence CYP3A4 gene expression, but stimulated CYP1A2 gene expression and inhibited GST expression. Also, the proliferation of human keratinocytes (NHK) was increased up to 150% by both amides 5 and 8; this stimulation was also detectable on the level of gene expression by an up-regulation of the transcription factor STAT6. The aliphatic aspartic compound 8 showed strong antiadhesive properties on the adhesion of Helicobacter pylori to human stomach tissue.

Published
2007
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9. Aqueous extracts and polysaccharides from liquorice roots (Glycyrrhiza glabra L.) inhibit adhesion of Helicobacter pylori to human gastric mucosa.

Author

Wittschier N, Faller G, and Hensel A

Subjects
Acids isolation & purification, Acids pharmacology, Bacterial Adhesion drug effects, Helicobacter pylori physiology, Humans, Plant Extracts chemistry, Plant Roots, Polysaccharides isolation & purification, Anti-Bacterial Agents pharmacology, Gastric Mucosa drug effects, Glycyrrhiza chemistry, Helicobacter pylori drug effects, Plant Extracts pharmacology, Polysaccharides pharmacology, Porphyromonas gingivalis drug effects
Abstract

Aims: Aqueous extracts from the roots of Glycyrrhiza glabra L. (Fabaceae) are widely used for treatment of stomach ulcer. The clinical proven effects are related to the presence of anti-inflammatory 12-keto-triterpensaponins in the extracts. Apart from that the influence of Glycyrrhiza glabra extract on the bacterial adhesion of Helicobacter pylori to stomach tissue was to be investigated. Additionally the influence of Glycyrrhiza glabra secondary compounds on the bacterial adhesion of Porphyromonas gingivalis, a major pathogen for induction of periodontal inflammations was to be investigated., Methodology: In vitro cytotoxicity against Helicobacter pylori was investigated by agar diffusion assay; antiadhesive properties of aqueous extract, raw polysaccharides and purified polysaccharide fractions was investigated by means of an in situ adhesion assay with FITC-labelled bacteria on tissue slides of human stomach resectates., Results: Aqueous extract (1mg/mL) of Glycyrrhiza glabra significantly inhibited the adhesion of Helicobacter pylori to human stomach tissue. This effect was related to the polysaccharides isolated from the extract, with one purified acidic fraction (0.25 SPB) as main active polymer. Purified polysaccharides did not exhibit direct cytotoxic effects against Helicobacter pylori and did not influence hemagglutination. Additionally raw polysaccharides from Glycyrrhiza glabra were shown to have strong antiadhesive effects against Porphyromonas gingivalis., Conclusion: Aqueous extracts and polysaccharides from the roots of Glycyrrhiza glabra are strong antiadhesive systems, which may be used as potent tools for a further development of cytoprotective preparations with anti-infectious potential.

Published
2009
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10. An extract of Pelargonium sidoides (EPs 7630) inhibits in situ adhesion of Helicobacter pylori to human stomach.

Author

Wittschier N, Faller G, and Hensel A

Subjects
Anti-Bacterial Agents administration & dosage, Anti-Bacterial Agents therapeutic use, Bacterial Adhesion drug effects, Helicobacter Infections drug therapy, Helicobacter pylori physiology, Humans, Microbial Sensitivity Tests, Plant Extracts administration & dosage, Plant Extracts therapeutic use, Plant Roots, Stomach microbiology, Anti-Bacterial Agents pharmacology, Helicobacter pylori drug effects, Pelargonium, Phytotherapy, Plant Extracts pharmacology
Abstract

Root extract from Pelargonium sidoides DC is used therapeutically as antimicrobial agent against infections of the respiratory system. In order to elucidate possible modes of actions we investigated the influence of P. sidoides root extract on microbial adhesion with Helicobacter pylori as model microorganism, a germ with a strong adherence to human stomach tissue. In an in-situ anti-adhesion assay intact human stomach tissue from patient resectates was incubated with fluorescent-labelled bacteria. Epithelial adhesion occurred in untreated samples and was quantified by fluorescent microscopy. Pre-treatment of the bacteria with Pelargonium extract showed good anti-adhesive activity. The antiadhesive effect was clearly dose-dependent in a range from 0.001 to 10 mg/ml. Within agar diffusion-test the extract had no direct cytotoxicity against H. pylori. The results show that the root extract from Pelargonium sidoides is a potent anti-adhesive agent against H. pylori and could therefore be a useful choice to avoid the first step of a bacterial infection.

Published
2007
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