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128 results on '"Winkler, Laura"'

3. The low mutational flexibility of the EPSP synthase in Bacillus subtilis is due to a higher demand for shikimate pathway intermediates

Author

Schwedt, Inge, primary, Schöne, Kerstin, additional, Eckert, Maike, additional, Pizzinato, Manon, additional, Winkler, Laura, additional, Knotkova, Barbora, additional, Richts, Björn, additional, Hau, Jann‐Louis, additional, Steuber, Julia, additional, Mireles, Raul, additional, Noda‐Garcia, Lianet, additional, Fritz, Günter, additional, Mittelstädt, Carolin, additional, Hertel, Robert, additional, and Commichau, Fabian M., additional

Published
2023
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6. A multi-model approach for wooden furniture failure under mechanical load

Author

Chevalier Luc, Pled Florent, Winkler Laura, Wilquin François, and Launay Eric

Subjects
wooden furniture, junction strength, multi-model, failure criteria, Materials of engineering and construction. Mechanics of materials, TA401-492
Abstract

Furniture failure generally appears at junctions between wooden parts. Failure prediction is a challenging problem considering the various technologies used for the assembly, the geometric dimensions of the wooden assembled parts and of the assembly components as well as the material properties of the wooden parts. Being able to provide a procedure for failure analysis is of great interest to the furniture industry. This paper proposes a multi-model approach in 3 steps: (i) a simplified global modeling of the whole structure (high loft bed) taking into account the specific geometry of each wooden part (beams or plates), (ii) a three-dimensional local numerical analysis of a through-bolt junction subjected to the mean critical load identified during a series of experimental compression tests to determine the local stresses in such a corner-type junction, and (iii) the application of an ad hoc failure criterion adapted to the anisotropic behavior of wood for failure prediction in through-bolt junctions.

Published
2022
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9. MicroRNA regulation of the serine synthesis pathway in endocrineresistant breast cancer cells.

Author

Petri, Belinda J., Piell, Kellianne M., Wilt, Ali E., Howser, Alexa D., Winkler, Laura, Whitworth, Mattie R., Valdes, Bailey L., Lehman, Norman L., Clem, Brian F., and Klinge, Carolyn M.

Subjects
RNA regulation, BREAST cancer, HORMONE receptor positive breast cancer, RNA-binding proteins, CANCER cells, SERINE, ESTROGEN
Abstract

Despite the successful combination of therapies improving survival of estrogen receptor a (ER+) breast cancer patients with metastatic disease, mechanisms for acquired endocrine resistance remain to be fully elucidated. The RNA binding protein HNRNPA2B1 (A2B1), a reader of N(6)-methyladenosine (m6A) in transcribed RNA, is upregulated in endocrineresistant, ER+ LCC9 and LY2 cells compared to parental MCF-7 endocrine-sensitive luminal A breast cancer cells. The miRNA-seq transcriptome of MCF-7 cells overexpressing A2B1 identified the serine metabolic processes pathway. Increased expression of two key enzymes in the serine synthesis pathway (SSP), phosphoserine aminotransferase 1 (PSAT1) and phosphoglycerate dehydrogenase (PHGDH), correlates with poor outcomes in ER+ breast patients who received tamoxifen (TAM). We reported that PSAT1 and PHGDH were higher in LCC9 and LY2 cells compared to MCF-7 cells and their knockdown enhanced TAM sensitivity in these-resistant cells. Here we demonstrate that stable, modest overexpression of A2B1 in MCF-7 cells increased PSAT1 and PHGDH and endocrine resistance. We identified four miRNAs downregulated in MCF-7-A2B1 cells that directly target the PSAT1 3'UTR (miR-145-5p and miR-424-5p), and the PHGDH 3'UTR (miR-34b-5p and miR-876-5p) in dual luciferase assays. Lower expression of miR-145-5p and miR-424-5p in LCC9 and ZR-75-1-4-OHT cells correlated with increased PSAT1 and lower expression of miR-34b-5p and miR-876-5p in LCC9 and ZR-75-1-4-OHT cells correlated with increased PHGDH. Transient transfection of these miRNAs restored endocrinetherapy sensitivity in LCC9 and ZR-75-1-4-OHT cells. Overall, our data suggest a role for decreased A2B1-regulated miRNAs in endocrine resistance and upregulation of the SSP to promote tumor progression in ER+ breast cancer. [ABSTRACT FROM AUTHOR]

Published
2023
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10. Over-Expression of LEDGF/p75 in HEp-2 Cells Enhances Autoimmune IgG Response in Patients with Benign Prostatic Hyperplasia—A Novel Diagnostic Approach with Therapeutic Consequence?

Author

Liedtke, Victoria, primary, Rose, Laura, additional, Hiemann, Rico, additional, Nasser, Abdullah, additional, Rödiger, Stefan, additional, Bonaventura, Alena, additional, Winkler, Laura, additional, Sowa, Mandy, additional, Stöckle, Michael, additional, Schierack, Peter, additional, Junker, Kerstin, additional, and Roggenbuck, Dirk, additional

Published
2023
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27. Coastal cliff at Lenzer Bach on Jasmund Peninsula, Rügen Island (Pleistocene Stripe 4): reconstructed history of glacitectonic deformation based on fold geometry and microstructural mapping

Author

Mehlhorn, Paul, Winkler, Laura, Grabbe, Franziska-Charlotte, Kenzler, Michael, Gehrmann, Anna, Hüneke, Heiko, and Rother, Henrik

Abstract

A thrust-bound footwall syncline located within the proximal part of the southern Jasmund Glacitectonic Complex is investigated, exploring the spatio-temporal relationship between glacitectonic macro- and microstructures. Orientation and geometry of macroscale folds and thrust faults reveal a two-phased deformation history recorded by the sedimentary sequence. The deformation is a result of glacitectonic imbrication and subsequent ice flow across Jasmund Peninsula during the late Weichselian. Clast microfabrics preserved within the folded glacial diamicts reveal that till-internal deformation is mainly related to subglacial shearing within the glacier bed, which predates large-scale imbrication and folding.

Published
2019

33. Body Composition and Menstrual Status in Adults With a History of Anorexia Nervosa—At What Fat Percentage is the Menstrual Cycle Restored?

Author

Winkler, Laura Al-Dakhiel, Frølich, Jacob Stampe, Schulpen, Maya, and Støving, René Klinkby

Subjects
Adult, body composition, menstrual status, Adolescent, Body Composition/physiology, Menstruation/physiology, dual energy X-ray absorptiometry, Anorexia Nervosa/diagnostic imaging, anorexia nervosa, amenorrhea, Body Mass Index, Young Adult, Absorptiometry, Photon, Menstrual Cycle/physiology, Humans, Female
Abstract

OBJECTIVE: To study the association between body composition measures and menstrual status in a large sample of adult patients with a history of anorexia nervosa and to calculate the predicted probability of resumption of menstrual function. Furthermore, to establish whether fat percentage is superior to body mass index in predicting the resumption of menses.METHOD: One hundred and thirteen adult women with a history of anorexia nervosa underwent a dual energy X-ray absorptiometry (DXA) scan and completed questionnaires regarding medication prescription and menstrual function.RESULTS: Fifty percent of patients were expected to resume their menstrual function at a body mass index of 19 kg m(-2) or a fat percentage of 23%. Twenty-five percent of patients were expected to resume their menstrual function at body mass index 14 kg m(-2) or fat percentage 11%. Fat percentage and body mass index were equally capable of predicting the resumption of menses.DISCUSSION: Fat percentage and body mass index were positive predictors of the resumption of menses, however, body composition measured by dual energy X-ray absorptiometry was not superior to body mass index in predicting menstrual recovery, which is of great clinical relevance as body mass index is easier and cheaper to obtain. Body composition measures only account for one of numerous factors involved in the resumption of menses. Regression models based on our data had a R(2) value of 0.14, indicating that only 14% of the variation in menstrual recovery could be explained by the variables included. © 2016 Wiley Periodicals, Inc. (Int J Eat Disord 2016).

Published
2017

34. NBV:Anorexia nervosa og andre spiseforstyrrelser

Author

Støving, René Klinkby, Solis, Anette, Richelsen, Bjørn, Priezel, Hanne, Frølich, Jacob Stampe, Ibsen, Jenna Rosenqvist, Nielsen, Joan Bach, Al-Dakhiel Winkler, Laura, Poulsen, Morten Møller, and Holmager, Pernille

Published
2017

45. Body composition and menstrual status in adults with a history of anorexia nervosa-at what fat percentage is the menstrual cycle restored?

Author

Winkler, Laura Al‐Dakhiel, Frølich, Jacob Stampe, Schulpen, Maya, and Støving, René Klinkby

Subjects
*ADIPOSE tissues, *AMENORRHEA, *ANOREXIA nervosa, *BODY composition, *CHI-squared test, *CONFIDENCE intervals, *MENSTRUATION, *PROBABILITY theory, *QUESTIONNAIRES, *STATISTICS, *T-test (Statistics), *LOGISTIC regression analysis, *DATA analysis, *BODY mass index, *DISEASE duration, *DATA analysis software, *DESCRIPTIVE statistics, *PHOTON absorptiometry, *ODDS ratio, *MANN Whitney U Test
Abstract

ABSTRACT Objective To study the association between body composition measures and menstrual status in a large sample of adult patients with a history of anorexia nervosa and to calculate the predicted probability of resumption of menstrual function. Furthermore, to establish whether fat percentage is superior to body mass index in predicting the resumption of menses. Method One hundred and thirteen adult women with a history of anorexia nervosa underwent a dual energy X-ray absorptiometry (DXA) scan and completed questionnaires regarding medication prescription and menstrual function. Results Fifty percent of patients were expected to resume their menstrual function at a body mass index of 19 kg m−2 or a fat percentage of 23%. Twenty-five percent of patients were expected to resume their menstrual function at body mass index 14 kg m−2 or fat percentage 11%. Fat percentage and body mass index were equally capable of predicting the resumption of menses. Discussion Fat percentage and body mass index were positive predictors of the resumption of menses, however, body composition measured by dual energy X-ray absorptiometry was not superior to body mass index in predicting menstrual recovery, which is of great clinical relevance as body mass index is easier and cheaper to obtain. Body composition measures only account for one of numerous factors involved in the resumption of menses. Regression models based on our data had a R2 value of 0.14, indicating that only 14% of the variation in menstrual recovery could be explained by the variables included. © 2016 Wiley Periodicals, Inc.(Int J Eat Disord 2017; 50:370-377) [ABSTRACT FROM AUTHOR]

Published
2017
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