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3. Seventeenth sir peter freyer memorial lecture and surgical symposium: September 18th & 19th, 1992

Author

O’Gradaigh, D., Byrne, P. J., Gillen, P., Lawlor, P., Walsh, T. N., Hennessy, T. P. J., O’Sullivan, S. T., O’Sullivan, G. C., Kirwan, W. O., Li, H., Caldwell, M. T. P., Hone, S., Attwood, S. E. A., Kelly, I. P., Corrigan, T. P., Mulligan, E., Kerin, M. J., Williams, N. N., Cronin, K. J., Sadar, M. El, Dervan, P., Fitzpatrick, J. M., Gorey, T. F., Maher, M., Hehir, D., Horgan, A., Stuart, R., O’Donnell, J. A., Brady, M. P., O’Donoghue, J. M., Flynn, J. R., Doyle, J., Gallagher, M., Connolly, K., Barry, M., Davies, M. G, West, M., O’Broin, E., Connolly, J. A., Long, D., Shine, M. F., Lennon, F., Dawson, K. J., Novell, J. R., Burroughs, A. K., Rolles, K., Joyce, W. P., Dolan, J., Hyland, J., Traynor, O., Bennett, M., Tighe, O., Mulcahy, H., O’Donoghue, D., Bouchier-Hayes, D., Croke, D. T., Santos, G., Khoury, G., Winslet, M. C., Lewis, A. A. M., Beausang, E., Mealy, K., Joyce, L., McNicholls, M., McErlean, D., Stokes, M. A., Barry, K., Sullivan, R., Byrne, J., Callaghan, J., O’Gorman°, T., Given, H. F., Dudeney, M. S., Redmond, M. P., Deasy, J. M., Young, V. K., Watson, R. G. K., O’Kane, G., Murphy, K., McDowell, C., Khan, K., Al-Ghazal, S. K., McCann, J., Stuart, R. C., O’Connor, M., McCabe, J., O’Byrne, J., O’Farrell, D., Walsh, M., O’Beirne, J., O’Flannagan, S., McGuinness, A., Brady, O., Quinlan, W., McCabe, J. P., Curtin, B., Stephens, M., Stack, J., McCarthy, P., Schnall, M., Pollack, H., Lynch, T. H., Waymont, B., Dunn, J. A., Hughes, M. A., Wallace, D. M. A., McDermott, T. E. D., Grainger, R., Rogers, E., Corcoran, M., Bredin, H., Grimes, H., Lanigan, D., Roobottom, C., Dubbins, P. A., Choa, R. G., Creagh, T., Butler, M. R., O’Flynn, K. J., MacDonagh, R. P., Thomas, D. G., Dawson, K., Aitken, J., Cooke, B., Parbhoo, S. P., Cannon, P. M., Low, S. C., Dixon, A., Ellis, I. O., Elston, C. W., Blarney, R. W., Mulligan, E. D., Cronin, K., Stack, A., Ennis, J., Gorey, T. F., Abbaskoor, F., O’Donoghue, M. K., Fulton, G., Tanner, W. A., Keane, F. B. V., Joseph, B. V., Cunningham, F. O., Dowling, M., Conveney, E., Geraghty, J. G., Byrne, P., Clarke, G., Duffy, J., O’Higgins, N., O’Hanlon, D., Horgan, P. G., O’Brien, D., Phelan, C., Given, P., Barry, M., Kent, P., Sheehan, S., Colgan, M. P., Moore, D., Shanik, G., Kent, P., Murphy, P., Sheehan, S., Colgan, M. P., Moore, D., Shanik, G., Vashisht, R., Sian, M., Franks, P., O’Malley, M. K., Hone, S., Gul, Y., Waldron, D., Hederman, W. P., Hone, S., Gul, Y., Waldron, D., Hederman, W. P., Maher, M., Singh, H. P., Dias, S., Aherne, T., O’Sullivan, S. T., Hehir, D. J., O’Connor, M., O’Donnell, J. A., Brady, M. P., McKeever, J. A., Stokes, M. A., Bannon, C. A., Beausang, E., Mehigan, D., Keaveney, T. V., Browne, T. F., Sivananthan, U. M., Rees, M. R., Whittaker, S., Davies, G. A., Vashisght, R., Sharp, E., Coady, A., Sterpetti, A., Greenhalgh, R. M., O’Malley, M. K., O’Brien, D. P., Gough, D. B., Horgan, P. G., Phelan, C., Given, H. F., Regan, M. C., Efron, I. E., Kirk, S. I., Hurson, M., Wasserkrug, H. L., Barbul, A., Browne, T. F., Haynes, S., Davies, G. A., Thornton, J., Sparkes, J., Hill, A. D. K., Gillen, P., Walsh, T. N., Hennessy, T. P. J., Kelly, C. J., Gallagher, J., Modyka, L., Redmond, H. P., Daly, J. M., Austin, O. M., Redmond, H. P., Cunney, R. J., Grace, P. A., Bouchier-Hayes, D., Curran, C., Byrne, J., O’Donoghue, J., Horgan, P. G., Given, H. F., Stokes, M. A., Abernathy, M., Sharpe, N., Lucy, M., McDermott, E. W. D., Mercer, P. M., O’Higgins, N. J., Murugasu, G., Geraghty, J. G., Groeschel, A., Carmody, M., Donohue, J., Osborne, D. H., O’Brien, D. P., McLaughlin, M., Devlin, J., Phillips, J. P., Ellias, Y., Tahir, M., McKeever, J., Tighe, M., Lynch, V., Ahmed, M., Smyth, P. P. A., Hetherton, A. M., Cunningham, F. O., O’Hanlon, D., Horgan, P., Little, M., Given, H. F., Quill, D. S., Duncan, C. O., McKeever, J. A., Stokes, M. A., Lynch, V., O’Donnell, M., Hobby, J. A. E., Little, D., Murphy, M., Mealy, K., Burke, P., Broe, P., Al-Ghazal, S. K., McKiernan, M., Khan, K., McCann, J., Murphy, M., Mealy, K., Broe, P., O’Donnell, M., Hobby, J. A. E., Regan, M. C., Kirk, S. J., Hurson, M., Wasserkrug, H. L., Barbul, A., Cronin, K. J., Kerin, M. J., Williams, N. N., Attwood, S. E. A., Crowe, J., MacMathuna, P., Lennon, J., Corrigan, T., O’Connell, R., Fitzpatrick, J. M., Gorey, T. F., Browne, A., Quershi, A., Leahy, A., Courtney, G., Grace, P., Osborne, H., Bouchier-Hayes, D., Dudeney, M. S., Redmond, H. P., Grace, P. A., Bouchier-Hayes, D., Buckley, D. J., Hehir, D. J., Kirwan, W. O., Goggin, M., Joyce, W. P., Traynor, O., Hyland, J., Buckley, D. J., Hehir, D. J., Kirwan, W. O., Farrell, T. A., Geraghty, J., Keeling, F. K., Kelly, I. P., Attwood, S. E. A., O’Connell, P. R., Corrigan, T. P., Hill, A. D. K., Redmond, H. P., Naama, H., Grace, P. A., Bouchier-Hayes, D., Moore, E., Barry, E., Duffy, C., Hogan, P., Nee, G., Fahy, M., McKiernan, M., Kenny, D. P., McCann, J., Ellias, V., Gibney, E., Joyce, W., Traynor, O., Gaffney, E., Doyle, J., Dervan, P., McMahon, J., McCabe, M., Kelly, P., Leader, M., Timon, C. I., Gullane, P., Dardick, I., Redmond, H. P., McCarthy, I., Dudeney, M. S., Hill, A. D. K., Grace, P. A., Bouchier-Hayes, D., Williams, N. N., Joyce, W. P., Couse, N. F., Morrin, M., and Delaney, P. V.

Published
1994
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6. Proceedings of meeting held November 6th & 7th, 1992 in the Sir Charles Parsons Theatre, University of Limerick

Author

O’Sullivan, S. T., Reardon, C. M., Hardiman, C., O’Donnell, J. A., Kirwan, W. O., Brady, M. P., Kelly, I., Attwood, S. E. A., Corrigan, T. P., Burke, P., Fitzgerald, P., Keeling, F., Grace, P., Bouchier-Hayes, D., Creagh, T. A., Williams, N. N., Kerin, M. K., Cronin, K., Smith, J., Fitzpatrick, J. M., Syed, S., Surana, R., Guiney, E. J., Malone, F., Reynolds, J., Ellias, Y., Traynor, O., O’Donnell, B., Samsunden, R., Humphreys, W. G., Khan, K., Al-Ghazal, S. K., Al-Ghazal, S., McKiernan, M., McCann, J., McAvinchey, D., Fitzgerald, M., McDermott, S., Murphy, A., Mooney, E. F., Geraghty, J., O’Connell, M., Kent, P., Sarazen, A., Angerson, W., Reynolds, J. V., Murchan, P., Keane, F. B. V., Tanner, W. A., McCarthy, J., Watson, W., O’Donnell, R., Brindley, N., Creagh, T., Dolan, J., Leader, M., Monkhouse, S., Qureshi, A., Clements, B., Halliday, I., Irwin, P., McCaigue, M., Barclay, R., Rowlands, B. J., Watson, R. W. G., Redmond, H. P., McCarthy, J. C., Dudley, M. S., Croke, D. T., Kelly, C. J., Grace, P. A., Burke, P. E., Bouchier-Hayes, D. J., Morrin, M., Khan, F., Barrett, N., Pembroke, T., Williams, N., Delaney, P., Gerghty, J., Kay, E., Leahy, A., Tanner, W. A., Refsum, S. E., Norwood, W., Boston, V. E., O’Mahony, A., Collins, J. K., O’Sullivan, G., Ramsbottom, D., Collins, P. B., Anderson, A. H., Johnston, S., Byrne, J., Horgan, P. G., Kennedy, M., Callaghan, J., Given, H. F., Delaney, C., Couse, N., Horgan, P., Fitzpatrick, J., Gorey, T., Solomon, I., Stokes, N., Bohan, A., Young, A., Murphy, D., Mercer, P., O’Higgins, N., Kerin, M. J., Murray, J., Dowling, M., Dervan, P., Ennis, J., Gorey, T. F., Ahmed, M., Cunningham, F. O., Smyth, P. P. A., Hetherton, A. M., Murray, M., O’Higgins, N. J., Alvi, R., Lane, B., Lynch, G., Browne, H., Keeling, P., Joyce, W. P., Hyland, J. M., Rehman, L., O’Leary, B., Hamdy, A., Watson, R. G. K., Hegarty, J., Breslin, D., Delaney, C. P., Istarabadi, M., O’Donnell, A., Hussain, R., Luke, D. A., McGovern, E., O’Neill, J., Stokes, M. A., Keaveney, T. V., Rasheed, R., Khan, F. H., Egan, T. J., Drumm, J., O’Domhnaill, S., Delaney, P. V., Hill, G. L., Hill, A. D. K., Darzi, A., Menzies-Gow, N., Donnelly, M. J., Timon, C. I., McShane, D. P., Ninan, G. K., Rasheed, K., Puri, P., Coveney, E. C., Moloney, R., Fitzgerald, R. J., Geoghegan, J. G., Branch, M. S., Pappas, T. N., Cotton, P. B., and Ryan, D.

Published
1993
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7. Irish Society of Gastroenterology: Proceedings of meeting held November 1990

Author

Coleman, J., Hayes, D. Bouchier, Daw, M. A., O’More, R., Keane, C. T., O’Morain, C., Johnson G. W., Spencer E. F. A., Wilkinson A. J., Kennedy T. L., Collins, J. S. A., Bamford, K. B., Collins, B. J., Sloan, J. M., Moorehead, R. J., Love, A. H. G., McCarthy, C., Collins, R., Beattie, S., McDevitt, J., Feighery, C., O’Farrelly, C., Weir, D. G., Kelleher, D., Whelan, A., Williams, Y., Kellegher, D., Weir, D., Prabhakar, M. C., Daw, M., Keane, F., McDermott E. W. M., Duduric B., Brennan M. F., Gaffney, R., O’Leary, J., Doyle, T., Gaffney, A., Gough, D. B., Fearon, K. C. H., Winstanley, P., Carter, D. C., McAnena, O. J., Moore, F. A., Moore, E. E., Pogetti, R., Peterson, V. M., Abemathy, C. M., Parsons, P., Williams, N. N., Daly, J. M., Herlyn, M., Bouchier-Hayes, D., Course, N. F., Horgan, P. G., Fahy, A., Delaney, C., Fitzpatrick, J. M., Gorey, T. F., Jazrawl S., Walsh T. N., Byrne P. J., Lawlor P., Hennessy T. P. J., O’Sullivan, K. R., Mathias, P. M., McNicholas, M., Logan, M., Masterson, J., Gillen, P., Hyland, J., Beausang, E., Joyce, W. P., Williams, N., Maxwell, W., Reid, I., Sharpe, I., Geraghty, J. G., Tanner, W. A., Patchett, S., Mulcahy, H., Afdhal, N., O’Donoghue, D., Lohan, A., Darzi, A., Keane, F. B. V., Keeling, P. W. N., Gibney, R. C., Keane, R., Drumm, J., Egan, T. J., Delaney, P. V., O’Sullivan, G., Harte, P., Grace, P. A., Qureshi, A., Osbome, H., Bouchier-Hayes, D. J., Bamford, S. L., Geraghty, J., Keane, F. B., Coleman, J. E., Gleeson, A., Mealy, K., Barry, M., Traynor, O., Keating, J. J., Chua, A., Ah-Kion, S., McNulty, J., Heaney, L. G., Murphy, P., Connolly, J. H., Callender, M. E., MacMathuna, P., Farrant, M., Hourihane, D. O. B., Donaldson, P., Lombard, M., Westaby, D., Weir, D. G., Williams, Roger, Couse, N. F., Burke, J., Lennon, G., Delaneyc, C. P., O’Brien, S., O’Brien, A., Fitzgerald, M. X., Hegarty, J. E., Noonan, N., Healy, M., O’Moore, R., Corrigan, O. I., Keating, P. W. N., Li, H. U. I., Byrne, P. J., Lawlor, P., Stuart, R. C., Jazrawi, S., Walsh, T. N., Hennessy, T. P. J., Course, N. F., Wai, D., Jaeger, H., Jackson, A. M., Mitchell, C. J., MacFie, J., Delaney, C. P., McGeeney, K., Caldwell, M. T. P., O’Donoghue, D. P., Connolly J., Stuart R. C., Kay E., Gorey T., Keane R., McKiernan, P., Glasgow, J. F. T., Johnson, B. T., Ferguson, R., Davidson, I., Hurley, J., Keeling, P., Crowley, T., Long, A. A., O’Brien, D. P., Waldron, R. P., Shearer, M. J., Given, H. F., McEntee, G., Monson, J., Donohue, J., Nagomey, D., Trimble, K. C., Molloy, A. M., Scott, J. M., Kay, E., McKeever, J., and Hennessey, T. P. J.

Published
1993
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8. Irish society of gastroenterology: Proceedings of meeting held in university college, Galway in may 1892

Author

Brannigan, A., Williams, N. N., Grahn, M., Williams, N. S., Fitzpatrick, J. M., O’Connell, P. R., Soong, C. V., Blair, P., Halliday, M. I., Hood, J. M., Rowlands, B. J., D’sa, A. A. B. Barros, Cahill, R. J., Beattie, S., Hamilton, H., O’Morain, C., Kelly, S. J., O’Malley, K. E., Stack, W. A., O’Donoghue, D., Baird, A. W., Cronin, K. J., Kerin, M. J., Crowe, J., MacMathuna, P., Lennon, J., Gorey, T. F., Chua, A., O’Kane, V., Dinan, T. G., Keeling, P. W. N., Mulligan, E., Cronin, K. L., Dervan, P., Ireland, A., Murphy, D., O’Sullivan, G., Ryan, E., Kelly, P., Gilvarry, J., Sant, S., Fan, X. J., Chua, A., Shahi, C. N., O’Connell, M., Weir, D. G., Kelleher, D., McDevitt, J., O’Donoghue, J. M., Horgan, P. G., Byrne, W. J., McGuire, M., Given, H. F., Daw, M. A., Kavanagh, P., O’Mahony, P., Joy, T., Gleeson, F., Mullan, A., Gibney, M., Mannion, Anne, Stevens, F. M., McCarthy, C. F., Killeen, A. A., Murchan, P. M., Reynolds, J. V., Leonard, N., Marks, P., Keane, F. B. V., Tanner, W. A., O’Connell, M. A., Corridan, B., Collins, R., Shannon, R., Cahill, R., Joyce, W. P., Goggin, M., O’Donoghue, D., Hyland, J., Traynor, O., Qureshi, A., DaCosta, M., Brindley, N., Burke, P., Grace, P., Bouchier-Hayes, D., Leahy, A. L., Courtney, G., Osbome, H., O’Donovan, N., O’Donoghue, M., Collins, J. K., Morrissey, D., McCarthy, J. E., Redmond, H. P., Hill, A. D. K., Grace, P. A., Naama, H., Austin, O. M., Bouchier-Hayes, D. M., Daly, J. M., Mulligan, E., Fitzpatrick, J. M., Breslin, D., Delaney, C. P., O’Sullivan, S. T., O’Sullivan, G. C., Kirwan, W. O., Weir, C. D., McGrath, L. T., Maynard, S., Anderson, N. H., Halliday, M. I., D’sa, A. A. B. Barros, Gokulan, C., O’Gorman, T. A., Breshihan, E., Lam, Pin Yin, Skehill, R., Grimes, H., McKeever, J. A., Stokes, M. A., Mehigan, D., Keaveny, T. V., Meehan, J., Molloy, A., Q’Farrelly, C., Scott, J., Dudeney, M. S., Leahy, A., Grace., P. A., McEntee, G., Hcaton, N. D., Douglas, V., Mondragon, R., O’Grady, J., Williams, R., Tan, K. C., Xia, H. X., Keane, C. T., O’Morain, C. A., O’Mahony, A., O’Sullivan, G. C., Corbett, A., O’Mahony, A., Ireland, A., Harte, P., Mulcahy, H., Patchett, S., Stack, W., Gallagher, M., Connolly, K., Doyle, J., Flynn, J. R., Maher, M., Hehir, D., Horgan, A., Stuart, R., Brady, M. P., Johnston, P. W., Johnston, B. T., Collins, B. J., Collins, J. S. A., Love, A. H. G., Marshall, S. G., Parks, T. G., Spence, R. A. J., O’Connor, H. J., Cunnane, K., Duggan, M., MacMalhuna, P., Delaney, C. P., Kerin, M., Gorey, T. F., Attwood, S. E. A., Viani, L., Jeffers, M., Walsh, T. N., Byrne, P. J., Frazer, I., Hennessy, T. P. J., Hill, G. L., Dickey, W., McMillan, S. A., Bharucha, C., Porter, K. G., Rolfe, H., Thornton, J., Attwood, S. E. A., Coleman, J., Stephens, R. B., Hone, S., Holmes, K., Kelly, I. P., Corrigan, T. P., McCrory, D., McCaigue, M., Barclay, G. R., Stack, W. A., Quirke, M., Hegarty, J. E., O’Donoghue, D. P., O’Hanlon, D., and Byrne, J.

Published
1992
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9. Irish society of gastroenterology: Proceedings Of Meeting Held

Author

Cotter, L. A., Healy, M., Buckley, M., O’Morain, C., Keane, C., O’Moore, R. R., Dickey, W., Roberts, G., Orr, G., Porter, K., McCrory, D., Halliday, M. I., Hoper, M., Crockard, A., Rowlands, B. J., Chua, A., Dinan, T., Dunbar, B., Weir, D. G., Keeling, P. W. N., Johnston, B. T., Collins, J. S. A., McFarland, R. J., Love, A. H. G., Darzi, A., Speakman, C. T. N., Spigelman, A., Henry, M. M., fnTanner, W. A., fnMcEntee, G. P., fnKeane, F. B., Tighe, O., Bennett, M., Mulcahy, H., Williams, N. N., Duignan, J. P., Bouchier-Hayes, D., O’Donoghue, D., Croke, D. T., Hill, A. D., Walsh, T. N., Hennessy, T. P. J., Goggin, M., Joyce, W. P., Prendergast, C., Gibney, E., Traynor, O. J., Hyland, J., O’Brien, S., Fitzgerald, M. X., Hegarty, J. E., Leahy, A., Grace, P., Qureshi, A., Leader, M., Broe, P., Eustace, S., Blake, N., McDevitt, J., Feighery, C. F., O’Farrelly, C., Kelleher, D., O’Connell, M. A., Stokes, M. A., Hill, G. L., Gaffney, P., O’Leary, J., Doyle, C., Hogan, J., Gaffney, Anne, Attwood, S. E. A., Murphy, P., Stephens, R. B., Wilson, R. H., Gilliland, R., Kee, F., Sloan, J. M., Moorehead, R. J., ’Suilleabhain, G., Horgan, A., Kirwan, W. O., Deans, G. T., Heatley, M., Williamson, K., Parks, T. G., Rowland, B. J., Spence, R. A. J., Mealy, K., Burke, P., Herlyn, M., Redmond, H. P., Clery, A. P., Deasy, J. M., Austin, O., Meenan, J., Canili, R. J., Mathias, P. M., Beattie, S., Hamilton, H., Geoghegan, J. G., Cheng, C. A., Lawson, D. C., Pappas, T. N., Collins, R., Beatie, S., Collins, J. K., O’Sullivan, G., Corbett, A., Clements, W. D. B., MacMathuna, P., Lombard, M., Gimson, A., Westaby, D., Williams, R., Duggan, M., Lennon, J., Crowe, J., Ritchie, A. J., Johnston, F., McGuigan, J., Gibbons, J. R. P., Buchanan, K. D., Gilvarry, J. M., Robinson, R., Fielding, J. F., Lawler, M., Humphries, P., Sheils, O., O’Briain, D. S., McCarthy, J., McDermott, M., Hourihane, D., Gallagher, H., Barry, M., Lennon, F., Hederman, W. P., O’Connell, P. R., Gorey, T. F., Fitzpatrick, J. M., Daly, J. M., Carthy, J. E., Redmond, H., Croake, D., Grace, P. A., Campbell, G., Maguire, O., Lynch, S., Atwood, J., Madrigal, L., Attwood, J., Murphy, A., Shovlin, P., Hegarty, J., Egleston, V., Mealy, K., MacErlean, D. P., Johnston, S., O’Malley, K., McEntee, G., Smyth, E., Moran, B., Plant, G., Rees, M., Brindley, N., Osborne, H., Lane, B., Lynch, G., Geraghty, J., Murphy, D., O’Brien, M., and Harte, P.

Published
1992
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10. Fifteenth Sir Peter Freyer Memorial Lecture and Surgical Symposium Proceedings of meeting held 14th & 15th September, 1990 at University College, Galway

Author

Bolger, C., Fry, G., Coakley, D., Philips, J., Sheahan, N., Malone, J., Gray, W. P., O’Sullivan, M., Buckley, T. F., O’Dwyer, T. P., Gullane, P. J., Kneafsey, B. P., Moran, K. T., O’Sullivan, S. T., Brady, M. P., Coveney, E. C., Geraghty, J. G., O’Higgins, N. J., O’Beirne, J., Seighe, P., McElwain, J. P., McCabe, J. P., Waldron, B., Byme, J., Waldron, B., Hickey, N., McCabe, J., McMahon, J., Colville, J., Moran, B. J., Frost, R. A., Kerin, M. J., Jaeger, J. J., Mitchell, C. J., MacFie, J., O’Hanrahan, T., Scott, N. A., Leinhardt, D., Irving, M. H., Gough, D., White, M., Morrin, M., Joyce, W., Phelan, D., Fitzpatrick, J., Gorey, T., Kerin, M. J., Wilkinson, D., Parkin, A., Kester, R. C., Gibney, E. J., McGrath, K., Cunningham, A. J., Bouchier-Hayes, D., Barry, M., Farrell, M., Monkhouse, W., Bouchier-Hayes, D., Dawson, K. J., Hehir, D., Hamilton, G., Grace, P. A., Quereschi, A., Keane, R., Broe, P., Bouchier-Hayes, D., Stansby, G., Hehir, D., Fuller, B., Hamilton, G., Connolly, A., O’Donnell, J., Little, D., Keane, R. M., Regan, M., Bouchier-Hayes, D., Horgan, P. G., Curran, C., O’Brien, D., Waldron, D., Mooney, E., Greally, J., Given, H. F., Duffy, M. J., Reilly, D., Coveney, E., Geraghty, J., Fennelly, J. J., O’Higgins, N., O’Hare, C. M., Jones, P. L., Zoma, T. A., Hemstreet, G. P., Postier, R. G., Coleman, J. E., Chaikof, E. L., Merrill, E. W., Callow, A. D., Williams, N. N., Daly, J. M., Herlyn, M., Bouchier-Hayes, D., Gaffney, R., Walsh, M., McShane, D., Timon, C., Hamilton, D., Connolly, J., Byrne, P. J., Stuart, R. B., Kay, E., Gorey, T., Hennessy, T. P. J., O’Leary, D. P., Booker, M., Scott, T. E., LaMorte, W. W., Geraty, J. G., Angerson, W. A., Carter, D. C., Lyons, J., Gough, D., Stack, A., Joyce, W., Gorey, T., Fitzpatrick, J. M., Kelly, C., Augustine, C., Kennedy, J., Creagh, T., Bouchier-Hayes, D., Mannion, D., Seigne, P., Fitzpatrick, G., Feeley, M., Butler, P., Grace, P., Leader, M., Curren, B., Barry-Walsh, C., Bouchier-Hayes, D., O’Brien, D., Horgan, P. G., Waldron, R., Shearer, M., Given, H. F., O’Rourke, S., Galea, M., Gilmour, A., Carter, R., Parkin, D., Blarney, R. W., Hehir, D. J., Parbhoo, S. P., Rothnie, N., Crowe, J., Wells, C., Dawson, K. J., Geraghty, J. G., Coveney, E. C., Duffy, M. J., Sherry, F., O’Higgins, N. J., Duffy, M. J., O’Grady, P., Coveney, E., Geraghty, J., Fennelly, J. J., O’Higgins, N. J., Byrne, J., Horgan, P. G., England, S., O’Callaghan, J., Given, H. F., Horgan, P. G., Waldron, D., O’Brien, D., Mooney, E., Grimes, H., Given, H. F., O’Brien, D., Horgan, P. G., Mooney, E., Waldron, D., Grimes, H., Given, H. F., Mulcahy, Ursula, Coveney, E. C., Smyth, P. P. A., McAlister, V., Geraghty, J. G., Murray, M. J., O’Higgins, M. J., Laoide, R. O., Coveney, E. C., Geraghty, J. G., Hourihane, J. B., O’Higgins, N. J., Mooney, E. F., Horgan, P. G., Brougham, C., Headon, D. R., Given, H. F., Coleman, C., Coveny, E. C., Laoide, R. O., Geraghty, J. G., Hourihane, J. B., O’Higgins, N. J., Jazawi, S., Walsh, T. N., Byrne, P. J., Lawlor, P., Li, H., Bolger, C., Sanfey, H., Hennessy, T. P. J., Joyce, W. P., Gough, D. B., Delaney, P. V., Gorey, T. F., Fitzpatrick, J. M., Attwood, S. E. A., Watson, A., Rogers, E., Waldron, R. P., Glynn, G., El-Bouri, K. U., Flynn, J., Keeling, P., Davies, M. G., Lavelle, J., Connolly, J., Shine, M. F., Lennon, F., Byrne, P. J., Stewart, R. C., Lawlor, P., Walsh, T. N., Hennessy, T. P., McKiernan, M. V., Johnston, J. G., Rogers, E., Greally, J., Hanrahan, L., Bredin, H. C., Corcoran, M. O., Norton, M., Rogers, E., Bredin, H. C., Corcoran, M. O., Flynn, R., Gleeson, M., Grainger, R., McDermott, T. E. D., Lanigan, D., McLean, P., Curran, B., Leader, M., Gleeson, M. J., Griffin, D. P., Gallagher, H. J., Creagh, T. A., Mulvin, D. M., Donovan, M. G., Murphy, D. M., McLean, P. A., Mulvin, D. W., Creagh, T. A., O’Brien, A., Murphy, D. M., O’Flynn, K. L., McDonagh, R., Thomas, D. G., Lynch, T. H., Anderson, P., Vaughan, A. T. M., Beaney, R. P., Wallace, D. M. A., Connolly, J., Solomon, L., Lavelle, J., Lennon, F., Shine, M. F., O’Riordain, D. S., O’Connell, P. R., Kirwan, W. O., Li, Hui, Byrne, P. J., Lawlor, P., Stuart, R. C., Jazrawi, S., Walsh, T. N., Hennessy, T. P. J., Koh, T. N., Sheehan, S. J., McKeever, J., Donohoe, J., Carmody, M., Osborne, D. H., Waldron, D. E., Rodgers, E., Patel, F., Horgan, P., Corcoran, M., Given, H. F., Walsh, K., Joyce, W. P., Gough, D. B., Gorey, T. F., Fitzpatrick, J. M., O’Donoghue, J. M., Waldron, R., Kerin, M. J., McCabe, J. P., McAnena, O. J., McGuire, M., Given, H. F., Smyth, J., Keye, G., Bahadursingh, A., Delaney, C., Joyce, W. P., Gough, D., Fitzpatrick, J. M., Gorey, T. F., Richie, A. J., Gibbons, J. R. P., O’Hanrahan, T., Marples, M., Banacewicz, J., Coleman, J. E., Troidl, H., Cassidy, L., Grace, P., Bouchier-Hayes, D., Prenderville, E. J., Burke, P. E., Colgan, M. -.P, Wee, B. L., Moore, D. J., Shanik, G. D., Cross, K. S., El-Sanadiki, M., Murray, J. J., Mikat, E., McCann, R., Hagen, P. -O., Cheatle, T. R., Steibe, E., Smith, P. D. Colebridge, Scurr, J. H., Barry, K., Waldron, D., Bresnihan, E., Courtney, D. F., Quill, D. S., Buckley, D., O’Riordan, D. S., O’Donncll, J. A., Gray, W. P., O’Donnell, J. A., Hill, A. D. K., O’Dwycr, P. J., MacErlean, D. P., Kerin, M. J., Couse, N. F., MacFie, J., Campbell, D., McBride, K., Geraghty, J. G., MacErlean, D., Murphy, J. J., Kirwan, W. O., Kaar, K., Docrat, H., Malik, S., Egan, J., Davidson, I. R., Hurley, J., Keeling, P., Rowley, H., Kaar, K., O’Sullivan, S. T., and Brady, M. P.

Published
1991
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13. Oncology

Author

Browell, D. A., Kirby, J. A., Gilmore, K., Shenton, B. K., Lennard, T. W. J., Orr, D. J. A., Hughes, L. E., Horgan, K., Darzi, A., Goldin, R., Guillou, P. J., Monson, J. R. T., Dworkin, M. J., Earlam, S., Allen-Mersh, T. G., Kerin, M. J., Mulligan, E., Williams, N. N., Cronin, K. J., Dervan, P., Fitzpatrick, J. M., Gorey, T. F., Reinbach, D. H., McGregor, J. R., O’Dwyer, P. J., Reynolds, J. V., Nolan, N., McCann, A., Duffy, M. J., McDermott, E. W. M., and O’Higgins, N. J.

Published
1992
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14. Colorectal

Author

Williams, N. N., Bennett, M., Tighe, O., Mulcahy, H., O’Donoghue, D., Bouchier-Hayes, D., Croke, D. T., Burke, P., Mealy, K., Gillen, P., Joyce, L., Traynor, O., Hyland, J., Byrne, D. J., Lavelle-Jones, M., Pringle, R., Kuzu, A., Lewis, W. G., Jones, D., Holdsworth, P. J., Finan, P. J., Johnston, D., Morgan, A. R., and Wastell, C.

Published
1992
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16. Royal Academy of Medicine in Ireland Section of Sugery: Proceedings of the registrars’ prize meeting held at the Royal College of Surgeons in Ireland on Friday 14th April 1989

Author

Reid, I. M., Monson, J. R. T., Keane, F. B. V., Tanner, W. A., Darzi, A., O’Morain, C., Taner, W. A., Reynolds, J. V., Daly, J. M., Williams, N. N., Daly, J. M., Herlyn, M., Moran, K., Kelly, D., Sheehan, S., Dervan, P., Fitzpatrick, J. M., Sheehan, S. J., Grace, P. A., Moran, K. T., Dowsett, D. J., Hurley, J. P., Tiernan, E., MacGowan, S., Lennon, F., Joyce, W. P., Provan, J. L., Ameli, F. M., McEwan, P., Jelenich, S., Jones, D. P., Jones, B. J., Fenton, D., Nowlan, P., Voorheis, H. P., Ryan, P. C., Butler, M. R., McEntee, G., McGeeney, K., Stuart, R. C., Marks, P., Lawlor, P., Byrne, P. J., Gorey, T. F., Hennessy, T. P. J., Kerin, M. J., Neilan, J., Waldron, R. P., McAnena, O. J., Gannon, F. X., and Given, H. F.

Published
1989
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17. The pros and cons of laparoscopic cholecystectomy and extracorporeal shock wave lithotripsy in the management of gallstone disease

Author

Darzi, A., Geraghty, J. G., Williams, N. N., Sheehan, S. S., Tanner, A. N., and Keane, F. B.

Subjects
Research Article
Abstract

The recent introduction of laparoscopic cholecystectomy (LAPC) has revolutionised the surgical treatment of gallstone disease. However, it has also raised doubts about the future role of extracorporeal shock wave lithotripsy (ESWL) in the treatment of gallstones. In this study, we compared patients treated successfully with ESWL and dissolution therapy with patients treated by LAPC. Out of 67 patients, 50 had successful clearance with ESWL while 50 out of 54 had successful LAPC. We evaluate treatment duration, recurrence rate (ESWL) and cost of treatment in both groups. All patients had uncomplicated symptomatic gallstones. The inclusion criteria were similar in both groups with the exception of patients with non-functioning gallbladders who were excluded from ESWL. The results of the study show that although ESWL is noninvasive and associated with minimal morbidity, it is also costly and has a high failure and recurrence rate. In contrast, laparoscopic cholecystectomy, while requiring short-term hospital stay and debility, seems to be a safe and effective alternative with an advantage in terms of cost and duration of treatment.

Published
1994

23. Tumor suppressor functions of ARLTS1 in lung cancers

Author

Tamotsu Kuroki, Carlo M. Croce, Manuela Ferracin, Masayoshi Shimizu, Kristoffel R. Dumon, Florencia Bullrich, Larry R. Kaiser, Sai Yendamuri, Cinzia Sevignani, Chang Gong Liu, Massimo Negrini, Shashi Rattan, George A. Calin, Noel N. Williams, Francesco Trapasso, Rossano Cesari, YENDAMURI S, TRAPASSO F, FERRACIN M, CESARI R, SEVIGNANI C, SHIMIZU M, RATTAN S, KUROKI T, DUMON K. R, BULLRICH F, LIU C. G, M. NEGRINI, WILLIAMS N. N, KAISER L. R, CROCE C. M, and CALIN G. A

Subjects
Cancer Research, Lung Neoplasms, Tumor suppressor gene, Molecular Sequence Data, Down-Regulation, Mice, Nude, Apoptosis, Cell Growth Processes, Biology, Adenoviridae, Mice, Cell Line, Tumor, medicine, Animals, Humans, Genes, Tumor Suppressor, Amino Acid Sequence, Lung cancer, Promoter Regions, Genetic, Conserved Sequence, A549 cell, Cell Growth Processe, Animal, Cell growth, ADP-Ribosylation Factors, Apoptosi, ADP-Ribosylation Factor, Promoter, Gene Therapy, Genetic Therapy, DNA Methylation, medicine.disease, Lung Neoplasm, Oncology, Cell culture, DNA methylation, Cancer research, Promoter Regions (Genetics), Sequence Alignment, Human
Abstract

ARLTS1 is a newly characterized tumor suppressor gene located at chromosome 13q14.3 and involved in the pathogenesis of various types of tumors: two single-nucleotide polymorphisms, one of them responsible for protein truncation, were found statistically associated with familial malignancies, whereas DNA hypermethylation and genomic deletions have been identified as a mechanism of ARLTS1 down-regulation in sporadic cancers. We found that in a large portion of lung carcinomas (37%) and in all analyzed lung cancer cell lines, ARLTS1 is strongly down-regulated due to DNA methylation in its promoter region. After its restoration by adenoviral transduction, ARLTS1-negative A549 and H1299 cells underwent apoptosis and inhibition of cell growth. Furthermore, ARLTS1 reexpression significantly reduced the ability of A549 and H1299 to form tumors in nude mice. Finally, we identified ∼650 transcripts differentially expressed after restoration of ARLTS1 expression in A549 cells, suggesting that various pathways involved in cell survival, proliferation, signaling, and development mediate the effects of wild-type ARLTS1 in a lung cancer system. [Cancer Res 2007;67(16):7738–45]

Published
2007

24. Utility of barium studies for patients with recurrent weight gain after Roux-en-Y gastric bypass.

Author

Wang B, Levine MS, Rubesin SE, Williams NN, Dumon K, and Raper S

Subjects
Adult, Aged, Barium Sulfate, Case-Control Studies, Contrast Media, Female, Gastric Bypass methods, Humans, Male, Middle Aged, Reoperation statistics & numerical data, Retrospective Studies, Treatment Failure, Weight Gain, Weight Loss, Anastomosis, Roux-en-Y adverse effects, Gastric Bypass adverse effects, Stomach pathology, Stomach surgery, Surgical Stapling adverse effects
Abstract

Aim: To determine the utility of barium studies for detecting abnormalities responsible for recurrent weight gain after gastric bypass surgery., Methods: A computerized search identified 42 patients who had undergone barium studies for recurrent weight gain after gastric bypass and 42 controls. The images were reviewed to determine the frequency of staple-line breakdown and measure the length/width of the pouch and gastrojejunal anastomosis. A large pouch exceeded 6 cm in length or 5 cm in width and a wide anastomosis exceeded 2 cm. Records were reviewed for the amount of recurrent weight gain and subsequent weight loss after additional treatment., Results: Staple-line breakdown was present in 6/42 patients (14%) with recurrent weight gain. When measurements were obtained, 13/35 patients (37%) with recurrent weight gain had a large pouch, three (9%) had a wide anastomosis, and four (11%) had both, whereas 22/42 controls (52%) had a large pouch, one (2%) had a wide anastomosis, and two (5%) had both. Ten patients (24%) with recurrent weight gain underwent staple-line repair (n = 3) or pouch/anastomosis revision (n = 7). These 10 patients had a mean weight loss of 38.1 lbs versus a mean loss of 8.6 lbs in 19 patients managed medically., Conclusion: Only 14% of patients with recurrent weight gain after gastric bypass had staple-line breakdown, whereas 57% had a large pouch, wide anastomosis, or both. Not all patients with abnormal anatomy had recurrent weight gain, but those who did were more likely to benefit from surgical intervention than from medical management., (Copyright © 2014 The Royal College of Radiologists. Published by Elsevier Ltd. All rights reserved.)

Published
2015
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25. The state of general surgery training: a different perspective.

Author

Foley PJ, Roses RE, Kelz RR, Resnick AS, Williams NN, Mullen JL, Kaiser LR, and Morris JB

Subjects
Humans, Internet, Quality of Life, Salaries and Fringe Benefits, Surveys and Questionnaires, United States, Workload, General Surgery education, Internship and Residency, Job Satisfaction
Abstract

Background: Much has been written about the influences of Accreditation Council for Graduate Medical Education (ACGME) work restrictions, the litigious climate in American medicine, and the proliferation of subspecialty fellowships on general surgery training. Few previous studies have addressed general surgical residents' perceptions of surgical training on a national level., Methods: A 38-question Institutional Review Board-approved survey was sent via e-mail to the program directors at all ACGME-approved general surgical training programs for distribution to categorical general surgery residents. Voluntary responses to statements focusing on job satisfaction, quality of life, and the influences of operative experience, work hours, fellows, physician extenders, as well as faculty and administration on resident training were solicited., Results: Overall, 997 responses were received from residents of all clinical levels from 40 states. Most respondents were from university-based programs (79%) with a broad representation of program sizes (mean of 6 graduates per year; range 2 to 11). Residents believe that they will be prepared to enter clinical practice at the conclusion of their training (86%), that the duration of surgical training is adequate (85%), and that they are exposed to sufficient case volume and complexity (85% and 84%, respectively). Only 360 respondents (36%) believe that they are financially compensated appropriately. Although most respondents support the ACGME work-hour restrictions (70%), far fewer feel that they improve their training or patient care (46.6% and 46.8%, respectively). Most respondents are proud to be surgical residents (88%), view surgery as a rewarding profession (87%), and would choose surgery as a profession again (77%)., Conclusions: Surgical residents are positive regarding the quality of their training and life, although they feel poorly compensated for their work. Most residents intend to pursue fellowship training. Survey responses were consistent irrespective of gender, ethnicity, and program type.

Published
2008
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26. Long term survival after pancreatic resection for pancreatic adenocarcinoma.

Author

Ahmad NA, Lewis JD, Ginsberg GG, Haller DG, Morris JB, Williams NN, Rosato EF, and Kochman ML

Subjects
Adult, Aged, Aged, 80 and over, Female, Humans, Male, Middle Aged, Survival Rate, Adenocarcinoma mortality, Adenocarcinoma surgery, Pancreatectomy mortality, Pancreatic Neoplasms mortality, Pancreatic Neoplasms surgery
Abstract

Objective: The aim of this study was to determine the long term survival of patients with pancreatic adenocarcinoma who underwent surgical resection and to assess the association of clinical, pathological, and treatment features with survival., Methods: Between January, 1990, and December, 1998, 125 patients underwent a pancreaticoduodenal or partial pancreatic resection for pancreatic ductal adenocarcinoma at our institution. The records of these patients were reviewed for demographics, tumor characteristics including size, histological grade, margin status, lymph node status, surgical TNM staging, and postoperative adjuvant therapy. The primary outcome variable analyzed was survival., Results: A total of 116 patients had complete follow-up and were included in the final analysis. The median survival after surgery was 16 months. The 1-, 3-, 5-, and 7-yr survival rates for all 116 patients were 60%, 23%, 19%, and 11%, respectively. The 1-, 3-, 5-, and 7-yr survival rates for patients who received adjuvant therapy were 69%, 28%, 23%, and 18% compared with 20% and 0% in patients who did not receive adjuvant therapy (p < 0.0001). The 1-, 3-, 5-, and 7-yr survival rates for patients with negative lymph nodes were 73%, 38%, 26%, and 22% compared with survival rates of 52%, 14%, 14%, and 9% in patients with positive lymph nodes (p = 0.01). In multivariate analyses, adjuvant therapy was the only feature found to be strongly associated with survival (hazards ratio = 0.26, 95% CI = 0.15-0.44)., Conclusions: The overall 5- and 7-yr survival rates of 19% and 11% in our study further validate that surgical resection in patients with pancreatic adenocarcinoma can result in long term survival, particularly when performed in association with adjuvant chemoradiation.

Published
2001
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27. Fragile histidine triad expression delays tumor development and induces apoptosis in human pancreatic cancer.

Author

Dumon KR, Ishii H, Vecchione A, Trapasso F, Baldassarre G, Chakrani F, Druck T, Rosato EF, Williams NN, Baffa R, During MJ, Huebner K, and Croce CM

Subjects
Adenoviridae genetics, Animals, Caspases metabolism, Cell Cycle physiology, Cell Division genetics, DNA Fragmentation, Female, Gene Transfer Techniques, Genes, Tumor Suppressor, Genetic Therapy, Genetic Vectors genetics, Humans, Mice, Mice, Nude, Mitochondria physiology, Pancreatic Neoplasms metabolism, Protein Biosynthesis, Signal Transduction physiology, Transduction, Genetic, Tumor Cells, Cultured, Xenograft Model Antitumor Assays, Acid Anhydride Hydrolases, Apoptosis genetics, Neoplasm Proteins, Pancreatic Neoplasms genetics, Pancreatic Neoplasms pathology, Proteins genetics
Abstract

The fragile histidine triad (FHIT) gene is a tumor suppressor gene that is altered by deletion in a large fraction of human tumors, including pancreatic cancer. To evaluate the potential of FHIT gene therapy, we developed recombinant adenoviral and adenoassociated viral (AAV) FHIT vectors and tested these vectors in vitro and in vivo for activity against human pancreatic cancer cells. Our data show that viral FHIT gene delivery results in apoptosis by activation of the caspase pathway. Furthermore, Fhit overexpression enhances the susceptibility of pancreatic cancer cells to exogenous inducers of apoptosis. In vivo results show that FHIT gene transfer delays tumor growth and prolongs survival in a murine model mimicking human disease.

Published
2001

28. Crohn's disease of the colon.

Author

Guy TS, Williams NN, and Rosato EF

Subjects
Colectomy, Colonic Diseases complications, Crohn Disease complications, Humans, Ileostomy, Intestinal Fistula etiology, Patient Selection, Proctocolectomy, Restorative, Treatment Outcome, Colonic Diseases surgery, Crohn Disease surgery
Abstract

The surgical treatment of Crohn's disease of the colon is distinct from that used in treating ulcerative colitis. Crohn's disease often involves the small bowel and is not "cured" by colorectal resection. The popular ileo-anal pouch procedures used in the management of ulcerative colitis generally are not used for the treatment of Crohn's colitis, because of higher complication rates. Commonly performed operations include ileostomy, segmental colon resection, subtotal colectomy, and proctocolectomy. The general surgeon, therefore, is provided with many options when faced with complications of Crohn's colitis. This article examines the attributes of and results reported for each of these options.

Published
2001
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29. Proliferating cell nuclear antigen: a new prognostic indicator in renal cell carcinoma.

Author

Cronin KJ, Williams NN, Kerin MJ, Creagh TA, Dervan PA, Smith JM, and Fitzpatrick JM

Subjects
Adult, Aged, Aged, 80 and over, Female, Humans, Male, Middle Aged, Prognosis, Proliferating Cell Nuclear Antigen, Survival Rate, Antigens, Neoplasm analysis, Carcinoma, Renal Cell mortality, Kidney Diseases mortality, Nuclear Proteins analysis
Abstract

Renal cell carcinoma is a tumor, the prognosis and behavior of which remain poorly understood. Proliferating cell nuclear antigen levels have been shown to act as an independent prognostic variable in a variety of malignancies. Proliferating cell nuclear antigen was evaluated in 59 cases of renal cell carcinoma, and the results were correlated with existing clinicopathological variables and survival. Proliferating cell nuclear antigen index (percentage of tumor cells positive for proliferating cell nuclear antigen) did not correlate with stage, grade or ploidy. To assess survival, tumors with proliferating cell nuclear antigen indexes of greater than and less than 60% were compared. The 24 patients with a high index (greater than 60%) had a significantly worse survival than did 35 with a low index (less than 60%, p < 0.001). Therefore, the prognostic potential of proliferating cell nuclear antigen in renal cell carcinoma is promising and may be of clinical value in the management of patients with renal cell carcinoma.

Published
1994
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30. The pros and cons of laparoscopic cholecystectomy and extracorporeal shock wave lithotripsy in the management of gallstone disease.

Author

Darzi A, Geraghty JG, Williams NN, Sheehan SS, Tanner AN, and Keane FB

Subjects
Adult, Aged, Bile Acids and Salts therapeutic use, Cholelithiasis drug therapy, Cholelithiasis surgery, Combined Modality Therapy, Female, Hospital Costs, Humans, Male, Middle Aged, Postoperative Complications, Recurrence, Cholecystectomy, Laparoscopic economics, Cholelithiasis therapy, Lithotripsy economics
Abstract

The recent introduction of laparoscopic cholecystectomy (LAPC) has revolutionised the surgical treatment of gallstone disease. However, it has also raised doubts about the future role of extracorporeal shock wave lithotripsy (ESWL) in the treatment of gallstones. In this study, we compared patients treated successfully with ESWL and dissolution therapy with patients treated by LAPC. Out of 67 patients, 50 had successful clearance with ESWL while 50 out of 54 had successful LAPC. We evaluate treatment duration, recurrence rate (ESWL) and cost of treatment in both groups. All patients had uncomplicated symptomatic gallstones. The inclusion criteria were similar in both groups with the exception of patients with non-functioning gallbladders who were excluded from ESWL. The results of the study show that although ESWL is noninvasive and associated with minimal morbidity, it is also costly and has a high failure and recurrence rate. In contrast, laparoscopic cholecystectomy, while requiring short-term hospital stay and debility, seems to be a safe and effective alternative with an advantage in terms of cost and duration of treatment.

Published
1994

31. Growth-factor-independence and invasive properties of colorectal carcinoma cells.

Author

Williams NN, Györfi T, Iliopoulos D, Herlyn D, Greenstein D, Linnenbach AJ, Daly JM, Jensen P, Rodeck U, and Herlyn M

Subjects
Animals, Cell Division drug effects, Cell Division physiology, Humans, Liver Neoplasms, Experimental secondary, Lung Neoplasms secondary, Mice, Mice, Inbred BALB C, Mice, Nude, Neoplasm Invasiveness pathology, Neoplasm Metastasis pathology, Neoplasm Transplantation, Phenotype, Tumor Cells, Cultured, Colorectal Neoplasms pathology, Growth Substances pharmacology
Abstract

During serial passage of the colorectal carcinoma cell line SW1116 in athymic nude mice, we selected 2 variants that metastasized to the lungs and liver. The metastatic capacity of these in vivo variant cell lines was associated with their ability to (1) grow under growth-factor-deprived conditions, (2) invade and transgress a reconstructed basement membrane with high effectiveness, and (3) produce higher activities of the substrate-degrading enzymes collagenase and plasminogen activator as compared to parental cells. To assess the relative contribution of growth-factor-independence and high levels of invasiveness/motility to the metastatic phenotype, variants of 6 colorectal carcinomas were selected in vitro by adaptation to a growth-factor-free culture medium followed by selection of highly invasive cells in chemoinvasion assays. Four out of 6 cell lines selected for growth-factor-independence showed significantly higher levels of invasiveness through reconstructed membranes, suggesting co-segregation of growth-factor-independence and high levels of invasiveness in vitro. Using an in vitro chemoinvasion assay, 2 poorly and 1 highly invasive cell line were further selected for invasiveness. After 6 selection passages, all cell lines were highly invasive and showed high motility rates. However, when injected s.c. into athymic nude mice to test their metastatic capacity in vivo, double-selected variant cell lines did not form spontaneous metastases. Our results indicate that growth-factor-independence and high levels of invasiveness, although associated with the metastatic phenotype, are not sufficient for experimental metastasis formation of colorectal carcinoma cells in vivo.

Published
1992
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32. Natural killer cell stimulatory factor (NKSF) augments natural killer cell and antibody-dependent tumoricidal response against colon carcinoma cell lines.

Author

Lieberman MD, Sigal RK, Williams NN 2nd, and Daly JM

Subjects
Animals, Carcinoma pathology, Colonic Neoplasms pathology, HLA Antigens analysis, Humans, Immunity, Cellular drug effects, In Vitro Techniques, Interleukin-12, Mice, Mice, Nude, Neoplasm Transplantation, Receptors, Fc metabolism, Tumor Cells, Cultured, Antibody-Dependent Cell Cytotoxicity drug effects, Carcinoma immunology, Colonic Neoplasms immunology, Interleukins pharmacology, Killer Cells, Natural immunology
Abstract

The therapy of colorectal cancer may be improved by biologic response modifiers that enhance natural killer (NK) cell and antibody-dependent tumoricidal mechanisms. This study examined the effect of a recently discovered cytokine purified from the supernatant of an Ebstein-Barr virus-transformed B-lymphoblastoid cell line (RPMI-8866), natural killer cell stimulatory factor (NKSF), on NK and antibody-dependent cellular cytotoxicity (ADCC) of human colon adenocarcinoma cell lines. Human peripheral blood lymphocytes were cultured for 24 hr in the presence or absence of NKSF (3.6 pM) or interleukin-2 (1 nM). The cultured lymphocytes were analyzed for lytic potential toward chromium-51-labeled colon carcinoma targets SW 1116, 498 LI, and WC 1. ADCC was measured by incubating chromium-51-labeled SW 1116 or WC 1 targets with the monoclonal antibody CO17-1A, an IgG2a antibody reactive with gastrointestinal cancer-associated cell antigen, or control mouse IgG prior to testing NKSF-treated or control PBL effectors in a 6-hr cytotoxicity assay. NKSF significantly enhanced NK cytolysis of colon carcinoma and NK-resistant lymphoma cell lines, and on a molar basis was approximately 300 times more potent than interleukin-2 in generating NK cytotoxicity. Furthermore, NKSF significantly augmented lymphocyte-mediated ADCC against colon carcinoma targets, and the combination of NKSF with the antibody CO17-1A had an additive effect on lymphocyte tumoricidial capacity. Thus, NKSF may have a potential role in the treatment of colon cancer.

Published
1991
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33. Transforming growth factor-alpha secretion by epidermal growth factor-dependent human tumor cell lines.

Author

Singletary SE, Williams NN, Rodeck U, Larry L, Tucker S, Spitzer G, and Herlyn M

Subjects
Carcinoma, Cell Line, Culture Media, Dose-Response Relationship, Drug, Female, Humans, Kinetics, Melanoma, Sarcoma, Transforming Growth Factor alpha metabolism, Uterine Cervical Neoplasms, Cell Division drug effects, Epidermal Growth Factor pharmacology, Transforming Growth Factor alpha biosynthesis
Abstract

Pairs of cell lines from spontaneous human tumors (cervical adenocarcinoma, melanoma, and synovial sarcoma) were established using serum-free culture conditions with and without exogenous epidermal growth factor (EGF). EGF-adapted cultures of melanoma and cervical adenocarcinoma origin secreted higher levels of bioactive transforming growth factor alpha (TGF-alpha) when compared to cultures maintained in the absence of EGF. Depletion of EGF for these EGF-adapted cultures resulted in growth arrest. In contrast, the sarcoma cell lines did not secrete TGF-alpha regardless of the culture conditions but EGF significantly stimulated proliferation of these cells in short-term assays. We show that exogenous EGF induces TGF-alpha production and supports proliferation of tumor cells of various tissue origin but is not essential for in vitro growth factor-deprived conditions.

Published
1990

34. Flow cytometry and prognostic implications in patients with solid tumors.

Author

Williams NN and Daly JM

Subjects
Breast Neoplasms pathology, Carcinoma pathology, Carcinoma, Transitional Cell genetics, Carcinoma, Transitional Cell pathology, Colorectal Neoplasms mortality, Colorectal Neoplasms pathology, Evaluation Studies as Topic, Head and Neck Neoplasms genetics, Head and Neck Neoplasms pathology, Humans, Melanoma genetics, Melanoma pathology, Neoplasm Recurrence, Local pathology, Ploidies, Prognosis, Sarcoma genetics, Sarcoma pathology, Skin Neoplasms genetics, Skin Neoplasms pathology, Urinary Bladder Neoplasms genetics, Urinary Bladder Neoplasms pathology, Biomarkers, Tumor analysis, Breast Neoplasms genetics, Carcinoma genetics, Colorectal Neoplasms genetics, DNA, Neoplasm analysis, Flow Cytometry methods, Neoplasm Recurrence, Local genetics
Abstract

Flow cytometric DNA content analysis is a rapid, quantitative method of determining the DNA ploidy status and proliferative index of a given tumor. Abnormal DNA content, or aneuploidy, has been recognized as a marker of malignancy and is present in about 70 per cent of solid tumors. In the majority of solid tumors, the consensus is that the presence of an aneuploid tumor predicts a poorer over-all survival rate and a shorter disease-free interval, indicating that patients with diploid tumors have a more favorable prognosis than those with aneuploid tumors. The prognostic implications of an abnormal DNA content, therefore, suggest either a higher risk of relapse, a worsening of survival rate or a risk for progression of disease in stages I and II carcinoma of the breast, carcinoma of the colon and rectum, superficial carcinoma of the bladder and malignant melanoma. Thus, the assessment of cellular DNA content should be regarded as an additional prognostic determinant and should play an ancillary role in the decisions regarding the management of patients with malignant disease. With the introduction of more sophisticated technology, it will be possible to simultaneously assay for DNA ploidy and cell cycle distribution in addition to a series of tumor markers, such as CEA, and various products of oncogenes, thus providing further understanding of the heterogeneity of solid tumor cells.

Published
1990

35. Current trends in management of hepatic metastases.

Author

Williams NN and Daly JM

Subjects
Antineoplastic Agents therapeutic use, Colorectal Neoplasms pathology, Humans, Liver Neoplasms diagnosis, Liver Neoplasms surgery, Neoplasm Staging, Liver Neoplasms secondary
Published
1989

36. Infusional versus systemic chemotherapy for liver metastases from colorectal cancer.

Author

Williams NN and Daly JM

Subjects
Adult, Aged, Antineoplastic Agents therapeutic use, Floxuridine administration & dosage, Hepatic Artery, Humans, Infusions, Intra-Arterial, Infusions, Intravenous, Liver Neoplasms secondary, Middle Aged, Antineoplastic Agents administration & dosage, Colorectal Neoplasms, Liver Neoplasms drug therapy
Abstract

It is clear from these studies that intrahepatic arterial infusion of floxuridine produces significantly higher response rates than does systemic infusion of the same drugs. Nevertheless, survival is the most important consideration when comparing two methods of treatment, and at present, there is only a slightly improved survival rate in those patients who receive intrahepatic infusional therapy. However, palliation of the patient and the effect on the quality of life should be taken into account, and these high response rates suggest an effective palliative measure to be offered to patients with this advanced disease. It is hoped that in the future the development of methods to identify those patients who will respond to this form of therapy, together with new ways to increase drug uptake by the tumors, will translate the better response rates achieved into better survival rates.

Published
1989
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37. Heparin kinetics in vascular surgery.

Author

Williams NN, Broe PJ, Burke P, Meagher EA, O'Donoghue C, Otridge B, and Bouchier-Hayes D

Subjects
Aged, Dose-Response Relationship, Drug, Heparin administration & dosage, Humans, Male, Middle Aged, Partial Thromboplastin Time, Prothrombin Time, Aortic Aneurysm surgery, Carotid Artery Diseases surgery, Endarterectomy, Heparin pharmacokinetics, Iliac Artery surgery
Abstract

Currently there is little information available about the efficacy of heparin during vascular surgery or of the effects of surgical trauma on heparin kinetics. This study was undertaken to evaluate the kinetics of heparin therapy during vascular surgery. Nine patients undergoing major vascular surgery (one carotid, one common iliac and seven aortic operations) were studied both preoperatively and intra-operatively, each patient acting as his own control. Following determination of control activated partial thromboplastin time (APTT) and plasma heparin levels, heparin (100 u/kg body weight) was administered intravenously. Heparin dosage ranged form 4500 units to 8600 units with a mean dose of 6500 units. Plasma heparin and APTT levels were then measured at 10 minute intervals for 1 hour and 20 minute intervals for a second hour. The mean pre-operative and intra-operative APTT levels at ten minutes attained maximal values of 6.6 +/- 3.7 and 8.8 +/- 1.7 times the control respectively. At the end of 2 hours the mean APTT remained greater than 2.5 times the control in both groups. Mean plasma heparin level was 0.83 +/- 0.04 units at 10 minutes and was almost identical in both groups. Heparin level was not a reliable indicator of anticoagulant effect as most patients achieved the same levels but had markedly differing APTT results. The results of this study suggest that excessive doses of heparin may be used in vascular surgery and that surgical trauma does not significantly alter sensitivity to heparin.(ABSTRACT TRUNCATED AT 250 WORDS)

Published
1989
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