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176 results on '"Willems, Rob J.L."'

1. Colonization of vancomycin-resistant Enterococcus faecium in human-derived colonic epithelium: Unraveling the transcriptional dynamics of host-enterococcal interactions

Author

MMB, UMC Utrecht Holding, Longziekten onderzoek 2, Onderzoek, Child Health, Regenerative Medicine and Stem Cells, MMB Research line 2, Beleid & Communicatie, Infection & Immunity, Stege, Paul B., Beekman, Jeffrey M., Hendrickx, Antoni P.A., Van Eijk, Laura, Rogers, Malbert R.C., Suen, Sylvia W.F., Vonk, Annelotte M., Willems, Rob J.L., Paganelli, Fernanda L., MMB, UMC Utrecht Holding, Longziekten onderzoek 2, Onderzoek, Child Health, Regenerative Medicine and Stem Cells, MMB Research line 2, Beleid & Communicatie, Infection & Immunity, Stege, Paul B., Beekman, Jeffrey M., Hendrickx, Antoni P.A., Van Eijk, Laura, Rogers, Malbert R.C., Suen, Sylvia W.F., Vonk, Annelotte M., Willems, Rob J.L., and Paganelli, Fernanda L.

Published
2024

2. YajC, a predicted membrane protein, promotes Enterococcus faecium biofilm formation in vitro and in a rat endocarditis model

Author

MMB Research line 2, Infection & Immunity, MMB, MS Reumatologie/Immunologie/Infectie, Top, Janetta, Zhang, Xinglin, Hendrickx, Antoni P.A., Boeren, Sjef, van Schaik, Willem, Huebner, Johannes, Willems, Rob J.L., Leavis, Helen L., Paganelli, Fernanda L., MMB Research line 2, Infection & Immunity, MMB, MS Reumatologie/Immunologie/Infectie, Top, Janetta, Zhang, Xinglin, Hendrickx, Antoni P.A., Boeren, Sjef, van Schaik, Willem, Huebner, Johannes, Willems, Rob J.L., Leavis, Helen L., and Paganelli, Fernanda L.

Published
2024

3. Pangenome-spanning epistasis and coselection analysis via de Bruijn graphs

Author

MMB Research line 2, Infection & Immunity, Kuronen, Juri, Horsfield, Samuel T., Pöntinen, Anna K., Mallawaarachchi, Sudaraka, Arredondo-Alonso, Sergio, Thorpe, Harry, Gladstone, Rebecca A., Willems, Rob J.L., Bentley, Stephen D., Croucher, Nicholas J., Pensar, Johan, Lees, John A., Tonkin-Hill, Gerry, Corander, Jukka, MMB Research line 2, Infection & Immunity, Kuronen, Juri, Horsfield, Samuel T., Pöntinen, Anna K., Mallawaarachchi, Sudaraka, Arredondo-Alonso, Sergio, Thorpe, Harry, Gladstone, Rebecca A., Willems, Rob J.L., Bentley, Stephen D., Croucher, Nicholas J., Pensar, Johan, Lees, John A., Tonkin-Hill, Gerry, and Corander, Jukka

Published
2024

7. Gut microbiome dynamics in index patients colonized with extended-spectrum beta-lactamase (ESBL)-producing Enterobacterales after hospital discharge and their household contacts

Author

MMB Research line 2, Infection & Immunity, Epi Infectieziekten Team 1, MMB, Medische Microbiologie, JC onderzoeksprogramma Infectieziekten, Top, Janetta, Verschuuren, Tess D., Viveen, Marco C., Riccio, M. Eugenia, Harbarth, Stephan, Kluytmans, Jan A.J.W., Willems, Rob J.L., Paganelli, Fernanda L., MMB Research line 2, Infection & Immunity, Epi Infectieziekten Team 1, MMB, Medische Microbiologie, JC onderzoeksprogramma Infectieziekten, Top, Janetta, Verschuuren, Tess D., Viveen, Marco C., Riccio, M. Eugenia, Harbarth, Stephan, Kluytmans, Jan A.J.W., Willems, Rob J.L., and Paganelli, Fernanda L.

Published
2023

8. The population-level impact of Enterococcus faecalis genetics on intestinal colonization and extraintestinal infection

Author

MMB Research line 2, Infection & Immunity, Datamanagement Team 1, Chaguza, Chrispin, Pöntinen, Anna K., Top, Janetta, Arredondo-Alonso, Sergio, Freitas, Ana R., Novais, Carla, Torres, Carmen, Bentley, Stephen D., Peixe, Luisa, Coque, Teresa M., Willems, Rob J.L., Corander, Jukka, MMB Research line 2, Infection & Immunity, Datamanagement Team 1, Chaguza, Chrispin, Pöntinen, Anna K., Top, Janetta, Arredondo-Alonso, Sergio, Freitas, Ana R., Novais, Carla, Torres, Carmen, Bentley, Stephen D., Peixe, Luisa, Coque, Teresa M., Willems, Rob J.L., and Corander, Jukka

Published
2023

9. Gut Colonization by ESBL-Producing Escherichia coli in Dogs Is Associated with a Distinct Microbiome and Resistome Composition

Author

MMB RZN, Infection & Immunity, MMB Research line 2, Stege, Paul B., Hordijk, Joost, Sandholt, Arnar K.S., Zomer, Aldert L., Viveen, Marco C., Rogers, Malbert R.C., Salomons, Moniek, Wagenaar, Jaap A., Mughini-Gras, Lapo, Willems, Rob J.L., Paganelli, Fernanda L., MMB RZN, Infection & Immunity, MMB Research line 2, Stege, Paul B., Hordijk, Joost, Sandholt, Arnar K.S., Zomer, Aldert L., Viveen, Marco C., Rogers, Malbert R.C., Salomons, Moniek, Wagenaar, Jaap A., Mughini-Gras, Lapo, Willems, Rob J.L., and Paganelli, Fernanda L.

Published
2023

10. Characterization of blaKPC-2 and blaNDM-1 Plasmids of a K. pneumoniae ST11 Outbreak Clone

Author

CMM Groep De Ridder, Cancer, MMB Research line 2, Infection & Immunity, Boralli, Camila Maria dos Santos, Paganini, Julian Andres, Meneses, Rodrigo Silva, Mata, Camila Pacheco Silveira Martins da, Leite, Edna Marilea Meireles, Schürch, Anita C., Paganelli, Fernanda L., Willems, Rob J.L., Camargo, Ilana Lopes Baratella Cunha, CMM Groep De Ridder, Cancer, MMB Research line 2, Infection & Immunity, Boralli, Camila Maria dos Santos, Paganini, Julian Andres, Meneses, Rodrigo Silva, Mata, Camila Pacheco Silveira Martins da, Leite, Edna Marilea Meireles, Schürch, Anita C., Paganelli, Fernanda L., Willems, Rob J.L., and Camargo, Ilana Lopes Baratella Cunha

Published
2023

11. Pangenome-spanning epistasis and coselection analysis via de Bruijn graphs

Author

Kuronen, Juri, Horsfield, Samuel T., Po¨ntinen, Anna K., Mallawaarachchi, Sudaraka, Arredondo-Alonso, Sergio, Thorpe, Harry, Gladstone, Rebecca A., Willems, Rob J.L., Bentley, Stephen D., Croucher, Nicholas J., Pensar, Johan, Lees, John A., Tonkin-Hill, Gerry, and Corander, Jukka

Abstract

Studies of bacterial adaptation and evolution are hampered by the difficulty of measuring traits such as virulence, drug resistance, and transmissibility in large populations. In contrast, it is now feasible to obtain high-quality complete assemblies of many bacterial genomes thanks to scalable high-accuracy long-read sequencing technologies. To exploit this opportunity, we introduce a phenotype- and alignment-free method for discovering coselected and epistatically interacting genomic variation from genome assemblies covering both core and accessory parts of genomes. Our approach uses a compact colored de Bruijn graph to approximate the intragenome distances between pairs of loci for a collection of bacterial genomes to account for the impacts of linkage disequilibrium (LD). We demonstrate the versatility of our approach to efficiently identify associations between loci linked with drug resistance and adaptation to the hospital niche in the major human bacterial pathogens Streptococcus pneumoniaeand Enterococcus faecalis.

Published
2024
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13. The population-level impact of Enterococcus faecalis genetics on intestinal colonisation and extraintestinal infection

Author

Chaguza, Chrispin, primary, Pöntinen, Anna K., additional, Top, Janetta, additional, Arredondo-Alonso, Sergio, additional, Freitas, Ana R., additional, Novais, Carla, additional, Torres, Carmen, additional, Bentley, Stephen D., additional, Peixe, Luisa, additional, Coque, Teresa M., additional, Willems, Rob J.L., additional, and Corander, Jukka, additional

Published
2022
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14. Species-Specific Patterns of Gut Metabolic Modules in Dutch Individuals with Different Dietary Habits

Author

MMB Research line 2, Infection & Immunity, Shetty, Sudarshan A., Stege, Paul B., Hordijk, Joost, Gijsbers, Esther, Dierikx, Cindy M., Van Duijkeren, Engeline, Franz, Eelco, Willems, Rob J.L., Paganelli, Fernanda L., Fuentes, Susana, MMB Research line 2, Infection & Immunity, Shetty, Sudarshan A., Stege, Paul B., Hordijk, Joost, Gijsbers, Esther, Dierikx, Cindy M., Van Duijkeren, Engeline, Franz, Eelco, Willems, Rob J.L., Paganelli, Fernanda L., and Fuentes, Susana

Published
2022

15. The interplay between community and hospital Enterococcus faecium clones within health-care settings: a genomic analysis

Author

MMB Research line 2, Infection & Immunity, van Hal, Sebastiaan J., Willems, Rob J.L., Gouliouris, Theodore, Ballard, Susan A., Coque, Teresa M., Hammerum, Anette M., Hegstad, Kristin, Pinholt, Mette, Howden, Benjamin P., Malhotra-Kumar, Surbhi, Werner, Guido, Yanagihara, Katsunori, Earl, Ashlee M., Raven, Katherine E., Corander, Jukka, Bowden, Rory, Enterococcal Study Group, MMB Research line 2, Infection & Immunity, van Hal, Sebastiaan J., Willems, Rob J.L., Gouliouris, Theodore, Ballard, Susan A., Coque, Teresa M., Hammerum, Anette M., Hegstad, Kristin, Pinholt, Mette, Howden, Benjamin P., Malhotra-Kumar, Surbhi, Werner, Guido, Yanagihara, Katsunori, Earl, Ashlee M., Raven, Katherine E., Corander, Jukka, Bowden, Rory, and Enterococcal Study Group

Published
2022

16. Effects of early-life antibiotics on the developing infant gut microbiome and resistome: a randomized trial

Author

Infectieziekten onderzoek3 (Bogaert), Immuno/reuma patientenzorg, MMB Research line 2, Infection & Immunity, Cluster B, Child Health, Reyman, Marta, van Houten, Marlies A., Watson, Rebecca L., Chu, Mei Ling J.N., Arp, Kayleigh, de Waal, Wouter J., Schiering, Irene, Plötz, Frans B., Willems, Rob J.L., van Schaik, Willem, Sanders, Elisabeth A.M., Bogaert, Debby, Infectieziekten onderzoek3 (Bogaert), Immuno/reuma patientenzorg, MMB Research line 2, Infection & Immunity, Cluster B, Child Health, Reyman, Marta, van Houten, Marlies A., Watson, Rebecca L., Chu, Mei Ling J.N., Arp, Kayleigh, de Waal, Wouter J., Schiering, Irene, Plötz, Frans B., Willems, Rob J.L., van Schaik, Willem, Sanders, Elisabeth A.M., and Bogaert, Debby

Published
2022

17. Impact of long-term dietary habits on the human gut resistome in the Dutch population

Author

MMB Research line 2, Infection & Immunity, MMB Zorg, Stege, Paul B., Hordijk, Joost, Shetty, Sudarshan A., Visser, Michael, Viveen, Marco C., Rogers, Malbert R.C., Gijsbers, Esther, Dierikx, Cindy M., van der Plaats, Rozemarijn Q.J., van Duijkeren, Engeline, Franz, Eelco, Willems, Rob J.L., Fuentes, Susana, Paganelli, Fernanda L., MMB Research line 2, Infection & Immunity, MMB Zorg, Stege, Paul B., Hordijk, Joost, Shetty, Sudarshan A., Visser, Michael, Viveen, Marco C., Rogers, Malbert R.C., Gijsbers, Esther, Dierikx, Cindy M., van der Plaats, Rozemarijn Q.J., van Duijkeren, Engeline, Franz, Eelco, Willems, Rob J.L., Fuentes, Susana, and Paganelli, Fernanda L.

Published
2022

19. The global dissemination of hospital clones of Enterococcus faecium

Author

van Hal, Sebastiaan J., Willems, Rob J.L., Gouliouris, Theodore, Ballard, Susan A., Coque, Teresa M., Hammerum, Anette M., Hegstad, Kristin, Westh, Hendrik T., Howden, Benjamin P., Malhotra-Kumar, Surbhi, Werner, Guido, Yanagihara, Katsunori, Earl, Ashlee M., Raven, Katherine E., Corander, Jukka, Bowden, Rory, Pinholt, Mette, Loens, Katherine, Xavier, Basil B., Matheeussen, Veerle, Goossens, Herman, van Hal, Sebastiaan J., Willems, Rob J.L., Gouliouris, Theodore, Ballard, Susan A., Coque, Teresa M., Hammerum, Anette M., Hegstad, Kristin, Westh, Hendrik T., Howden, Benjamin P., Malhotra-Kumar, Surbhi, Werner, Guido, Yanagihara, Katsunori, Earl, Ashlee M., Raven, Katherine E., Corander, Jukka, Bowden, Rory, Pinholt, Mette, Loens, Katherine, Xavier, Basil B., Matheeussen, Veerle, and Goossens, Herman

Abstract

Background: The hospital-adapted A1 group of Enterococcus faecium remains an organism of significant concern in the context of drug-resistant hospital-associated infections. How this pathogen evolves and disseminates remains poorly understood. Methods: A large, globally representative collection of short-read genomic data from the hospital-associated A1 group of Enterococcus faecium was assembled (n = 973). We analysed, using a novel analysis approach, global diversity in terms of both the dynamics of the accessory genome and homologous recombination among conserved genes. Results: Two main modes of genomic evolution continue to shape E. faecium: the acquisition and loss of genes, including antimicrobial resistance genes, through mobile genetic elements including plasmids, and homologous recombination of the core genome. These events lead to new clones emerging at the local level, followed by the erosion of signals of clonality through recombination, and in some identifiable cases producing new clonal clusters. These patterns lead to new, emerging lineages which are able to spread globally over relatively short timeframes. Conclusions: The ability of A1 E. faecium to continually present new combinations of genes for potential selection suggests that controlling this pathogen will remain challenging but establishing a framework for understanding genomic evolution is likely to aid in tracking the threats posed by newly emerging lineages.

Published
2021

21. Recovering escherichia coli plasmids in the absence of long-read sequencing data

Author

Infection & Immunity, MMB Research line 2, MMB opleiding Arts microbioloog, Biostatistiek Onderzoek, Paganini, Julian A., Plantinga, Nienke L., Arredondo-alonso, Sergio, Willems, Rob J.L., Schürch, Anita C., Infection & Immunity, MMB Research line 2, MMB opleiding Arts microbioloog, Biostatistiek Onderzoek, Paganini, Julian A., Plantinga, Nienke L., Arredondo-alonso, Sergio, Willems, Rob J.L., and Schürch, Anita C.

Published
2021

22. Erratum for Janssen et al., 'Evolution of Colistin Resistance in the Klebsiella pneumoniae Complex Follows Multiple Evolutionary Trajectories with Variable Effects on Fitness and Virulence Characteristics': Evolution of colistin resistance in the klebsiella pneumoniae complex follows multiple evolutionary trajectories with variable effects on fitness and virulence characteristics (Antimicrobial Agents and Chemotherapy (2021) 65:1 (e01958-20) DOI: 10.1128/AAC.01958-20)

Author

MMB Research line 2, MMB Research line 1, Infection & Immunity, MMB Zorg, Epi Infectieziekten, JC onderzoeksprogramma Infectieziekten, Janssen, Axel B., Doorduijn, Dennis J., Mills, Grant, Rogers, Malbert R.C., Bonten, Marc J.M., Rooijakkers, Suzan H.M., Willems, Rob J.L., Bengoechea, Jose A., Van Schaik, Willem, MMB Research line 2, MMB Research line 1, Infection & Immunity, MMB Zorg, Epi Infectieziekten, JC onderzoeksprogramma Infectieziekten, Janssen, Axel B., Doorduijn, Dennis J., Mills, Grant, Rogers, Malbert R.C., Bonten, Marc J.M., Rooijakkers, Suzan H.M., Willems, Rob J.L., Bengoechea, Jose A., and Van Schaik, Willem

Published
2021

23. Differential PilA pilus assembly by a hospital-acquired and a community-derived Enterococcus faecium isolate

Author

Hendrickx, Antoni P.A., Schapendonk, Claudia M.E., van Luit-Asbroek, Miranda, Bonten, Marc J.M., van Schaik, Willem, and Willems, Rob J.L.

Subjects
Genes -- Research, Cross infection -- Risk factors, Cross infection -- Research, Nosocomial infections -- Risk factors, Nosocomial infections -- Research, Enterococcus -- Health aspects, Enterococcus -- Research, Biological sciences
Abstract

Pili are hair-like structures protruding from the cell envelope of bacterial cells. Here, we describe the conditional and differential display of PilA-type pili, and PilE and PilF proteins, encoded from pilin gene cluster 1 at the surface of a hospital-acquired Enterococcus faecium bloodstream isolate (E1 165) and a community-derived stool isolate (E1039), at two different temperatures. Both strains have virtually identical pilA gene clusters, as determined by sequencing. Western blotting and transmission immunoelectron microscopy revealed that PilA and PilF assembled into high-molecular-mass pilus-like structures at 37[degrees]C in the E1165 strain, whereas PilE was not produced at either of the temperatures used; at 21[degrees]C, PilA and PilF were cell-wall-anchored proteins. In contrast, in strain E1039, PilA, PilE and PilF pilin proteins were found to be displayed as cell-wall-anchored proteins at 37[degrees]C only, and they were not associated with pilus-like structures. The discrepancy in pilus assembly between E1039 and E1165 cannot be explained by differences in expression of the genes encoding the predicted sortases in the pilA gene cluster, as these had similar expression levels in both strains at 21 and 37[degrees]C. Double-labelling electron microscopy revealed that PilA formed the pilus backbone in E1165, and PilF the minor subunit which was distributed along the PilA pilus shaft and positioned at the tip; however, it was deposited as a cell-wall-anchored protein in a pilA isogenic mutant. The differential deposition of surface proteins from pilin gene cluster 1 and differences in pilus assembly in the two strains suggest a complex post-transcriptional regulatory mechanism of pilus biogenesis in E. faecium. DOI 10.1099/mic.0.041392-0

Published
2010

24. Genetic variation and evolution of the pathogenicity island of Enterococcus faecalis

Author

McBride, Shonna M., Coburn, Phillip S., Baghdayan, Arto S., Willems, Rob J.L., Grande, Maria J., Shankar, Nathan, and Gilmore, Michael S.

Subjects
Genetic variation -- Evaluation, Molecular evolution -- Evaluation, Bacterial proteins -- Genetic aspects, Bacterial proteins -- Properties, Enterococcus -- Chemical properties, Enterococcus -- Genetic aspects, Biological sciences
Abstract

Enterococcus faecalis is a leading cause of nosocomial infections and is known for its ability to acquire and transfer virulence and antibiotic resistance determinants from other organisms. A 150-kb pathogenicity island (PAI) encoding several genes that contribute to pathogenesis was identified among antibiotic-resistant clinical isolates. In the current study, we examined the structure of the PAI in a collection of isolates from diverse sources in order to gain insight into its genesis and dynamics. Using multilocus sequence typing to assess relatedness at the level of strain background and microarray analysis to identify variations in the PAI, we determined the extent to which structural variations occur within the PAI and also the extent to which these variations occur independently of the chromosome. Our findings provide evidence for a modular gain of defined gene clusters by the PAI. These results support horizontal transfer as the mechanism for accretion of genes into the PAI and highlight a likely role for mobile elements in the evolution of the E. faecalis PAI.

Published
2009

25. Expression of two distinct types of pill by a hospital-acquired Enterococcus faecium isolate

Author

Hendrickx, Antoni P.A., Bonten, Marc J.M., van Luit-Asbroek, Miranda, Schapendonk, Claudia M.E., Kragten, Angela H.M., and Willems, Rob J.L.

Subjects
Nosocomial infections -- Causes of, Cilia and ciliary motion -- Analysis, Cross infection -- Causes of, Enterococcus -- Structure, Enterococcus -- Research, Enterococcus -- Health aspects, Enterococcus -- Genetic aspects, Biological sciences
Abstract

Surface filamentous structures designated pill, and implicated in virulence, have been found on the surfaces of several Gram-positive pathogens. This work describes the conditional expression of two phenotypically distinct pilus-like structures, designated PilA and PilB, on the surface of a hospital-adapted Enterococcus faecium bloodstream isolate. E. faecium is an emerging Gram-positive opportunistic pathogen that can cause severe disease, particularly in immunocompromised patients. Expression of PilA- and PilB-type pill was analysed during different phases of growth in broth culture. During growth, PilA and PilB pilin subunits were expressed around the cross-wall in early-exponential-phase cells. Polymerization and migration of short PilB-type pill towards the poles occurred in cells from the exponential phase and long polymerized pill were expressed at the poles of cells grown to stationary phase. In contrast, PilA-type pill were not expressed in broth culture, but only when cells were grown on solid media. Furthermore, surface expression of the PilA- and PilB-type pill was regulated in a temperature-dependent manner, as polymerization of two distinct types of pill at the surface only occurred when cells were grown at 37 [degrees]C; no pili were observed on cells grown at 21 [degrees]C. Hospital-aquired E. faecium isolates were specifically enriched in pilin gene clusters, suggesting that conditional expression of pill may contribute to E. faecium pathogenesis.

Published
2008

26. Five genes encoding surface-exposed LPXTG proteins are enriched in hospital-adapted Enterococcus faecium clonal complex 17 isolates

Author

Hendrickx, Antoni P.A., van Wamel, Willem J.B., Posthuma, George, Bonten, Marc J.M., and Willems, Rob J.L.

Subjects
Enterococcus -- Genetic aspects, Cross infection -- Genetic aspects, Nosocomial infections -- Genetic aspects, Biological sciences
Abstract

Most Enterococcus faecium isolates associated with hospital outbreaks and invasive infections belong to a distinct genetic subpopulation called clonal complex 17 (CC17). It has been postulated that the genetic evolution of CC17 involves the acquisition of various genes involved in antibiotic resistance, metabolic pathways, and virulence. To gain insight into additional genes that may have favored the rapid emergence of this nosocomial pathogen, we aimed to identify surface-exposed LPXTG cell wall-anchored proteins (CWAPs) specifically enriched in CC17 E. faecium. Using PCR and Southern and dot blot hybridizations, 131 E. faecium isolates (40 CC17 and 91 non-CCl7) were screened for the presence of 22 putative CWAP genes identified from the E. faecium TX0016 genoine. Five genes encoding LPXTG surface proteins were specifically enriched in E. faecium CC17 isolates. These five LPXTG surface protein genes were found in 28 to 40 (70 to 100%) of CC17 and in only 7 to 24 (8 to 26%) of non-CC17 isolates (P < 0.05). Three of these CWAP genes clustered together on the E. faecium TX0016 genoine, which may eoinprise a novel enterococcal pathogenicity island covering E. faecium contig 609. Expression at the InRNA level was demonstrated, and iininunotransinission electron microscopy revealed an association of the five LPXTG surface proteins with the cell wall. Minimal spanning tree analysis based on the presence and absence of 22 CWAP genes revealed grouping of all 40 CC17 strains together with 18 hospital-derived but evolutionary unrelated non-CC17 isolates in a distinct CWAP-enriched cluster, suggesting horizontal transfer of CWAP genes and a role of these CWAPs in hospital adaptation.

Published
2007

27. Enterococcal surface protein Esp is important for biofilm formation of Enterococcus faecium E1162

Author

Heikens, Esther, Bonten, Marc J.M., and Willems, Rob J.L.

Subjects
Bacterial proteins -- Influence, Microbial mats -- Growth, Enterococcus -- Physiological aspects, Company growth, Biological sciences
Abstract

Enterococci have emerged as important nosocomial pathogens with resistance to multiple antibiotics. Adhesion to abiotic materials and biofilm formation on medical devices are considered important virulence properties. A single clonal lineage of Enterococcus faecium, complex 17 (CC17), appears to be a successful nosocomial pathogen, and most CC17 isolates harbor the enterococcal surface protein gene, esp. In this study, we constructed an esp insertion-deletion mutant in a clinical E. faecium CC17 isolate. In addition, initial adherence and biofilm assays were performed. Compared to the wild-type strain, the esp insertion-deletion mutant no longer produced Esp on the cell surface and had significantly lower initial adherence to polystyrene and significantly less biofilm formation, resulting in levels of biofilm comparable to those of an esp-negative isolate. Capacities for initial adherence and biofilm formation were restored in the insertion-deletion mutant by in trans complementation with esp. These results identify Esp as the first documented determinant in E. faecium CC17 with an important role in biofilm formation, which is an essential factor in infection pathogenesis.

Published
2007

29. Nonclonal Emergence of Colistin Resistance Associated with Mutations in the BasRS Two-Component System in Escherichia coli Bloodstream Isolates

Author

MMB Moleculair B, MMB Research line 2, Epi Infectieziekten, MMB, Infection & Immunity, JC onderzoeksprogramma Infectieziekten, Janssen, Axel B., Bartholomew, Toby L., Marciszewska, Natalia P., Bonten, Marc J.M., Willems, Rob J.L., Bengoechea, Jose A., van Schaik, Willem, MMB Moleculair B, MMB Research line 2, Epi Infectieziekten, MMB, Infection & Immunity, JC onderzoeksprogramma Infectieziekten, Janssen, Axel B., Bartholomew, Toby L., Marciszewska, Natalia P., Bonten, Marc J.M., Willems, Rob J.L., Bengoechea, Jose A., and van Schaik, Willem

Published
2020

30. Genomic rearrangements uncovered by genome-wide co-evolution analysis of a major nosocomial pathogen, enterococcus faecium

Author

MMB Research line 2, Infection & Immunity, Top, Janetta, Arredondo-Alonso, Sergio, Schürch, Anita C., Puranen, Santeri, Pesonen, Maiju, Pensar, Johan, Willems, Rob J.L., Corander, Jukka, MMB Research line 2, Infection & Immunity, Top, Janetta, Arredondo-Alonso, Sergio, Schürch, Anita C., Puranen, Santeri, Pesonen, Maiju, Pensar, Johan, Willems, Rob J.L., and Corander, Jukka

Published
2020

31. Whole Genome Sequence Analysis of the First Vancomycin-Resistant Enterococcus faecium Isolates from a Libyan Hospital in Tripoli

Author

MMB Front office, dLAB Biobank, MMB Research line 2, Infection & Immunity, Ahmed, Mohamed O., Elramalli, Asma K., Baptiste, Keith E., Daw, Mohamed A., Zorgani, Abdulaziz, Brouwer, Ellen, Willems, Rob J.L., Top, Janetta, MMB Front office, dLAB Biobank, MMB Research line 2, Infection & Immunity, Ahmed, Mohamed O., Elramalli, Asma K., Baptiste, Keith E., Daw, Mohamed A., Zorgani, Abdulaziz, Brouwer, Ellen, Willems, Rob J.L., and Top, Janetta

Published
2020

32. Low IgA Associated With Oropharyngeal Microbiota Changes and Lung Disease in Primary Antibody Deficiency

Author

MS Reumatologie/Immunologie/Infectie, Infection & Immunity, MS Radiologie, MS Interne Geneeskunde, Immuno/reuma patientenzorg, Child Health, MMB RZN, MMB Research line 2, MMB, Researchgr. Systems Radiology, Circulatory Health, Regenerative Medicine and Stem Cells, Cancer, Biostatistiek Onderzoek, JC onderzoeksprogramma Methodologie, Berbers, Roos Marijn, Mohamed Hoesein, Firdaus A.A., Ellerbroek, Pauline M., van Montfrans, Joris M., Dalm, Virgil A.S.H., van Hagen, P. Martin, Paganelli, Fernanda L., Viveen, Marco C., Rogers, Malbert R.C., de Jong, Pim A., Uh, Hae Won, Willems, Rob J.L., Leavis, Helen L., MS Reumatologie/Immunologie/Infectie, Infection & Immunity, MS Radiologie, MS Interne Geneeskunde, Immuno/reuma patientenzorg, Child Health, MMB RZN, MMB Research line 2, MMB, Researchgr. Systems Radiology, Circulatory Health, Regenerative Medicine and Stem Cells, Cancer, Biostatistiek Onderzoek, JC onderzoeksprogramma Methodologie, Berbers, Roos Marijn, Mohamed Hoesein, Firdaus A.A., Ellerbroek, Pauline M., van Montfrans, Joris M., Dalm, Virgil A.S.H., van Hagen, P. Martin, Paganelli, Fernanda L., Viveen, Marco C., Rogers, Malbert R.C., de Jong, Pim A., Uh, Hae Won, Willems, Rob J.L., and Leavis, Helen L.

Published
2020

33. gplas: a comprehensive tool for plasmid analysis using short-read graphs

Author

MMB RZN, MMB Research line 2, Epi Hart- en Vaatziekten Team 2, Epi Infectieziekten Team 1, Infection & Immunity, JC onderzoeksprogramma Infectieziekten, MMB, UMC Utrecht, Arredondo-Alonso, Sergio, Bootsma, Martin, Hein, Yaïr, Rogers, Malbert R.C., Corander, Jukka, Willems, Rob J.L., Schürch, Anita C., MMB RZN, MMB Research line 2, Epi Hart- en Vaatziekten Team 2, Epi Infectieziekten Team 1, Infection & Immunity, JC onderzoeksprogramma Infectieziekten, MMB, UMC Utrecht, Arredondo-Alonso, Sergio, Bootsma, Martin, Hein, Yaïr, Rogers, Malbert R.C., Corander, Jukka, Willems, Rob J.L., and Schürch, Anita C.

Published
2020

36. Global spread of vancomycin-resistant Enterococcus faecium from distinct nosocomial genetic complex

Author

Willems, Rob J.L., Top, Janetta, van Santen, Marga, Robinson, D. Ashley, Coque, Teresa M., Baquero, Fernando, Grundmann, Hajo, and Bonten, Marc J.M.

Subjects
Vancomycin -- Research, Drug resistance in microorganisms -- Research, Drug resistance in microorganisms -- Genetic aspects, Enterococcal infections -- Research, Enterococcal infections -- Drug therapy, Enterococcal infections -- Genetic aspects
Abstract

Vancomycin-resistant enterococci (VRE) have caused hospital outbreaks worldwide, and the vancomycin-resistance gene (vanA) has crossed genus boundaries to methicillin-resistant Staphylococcus aureus. Spread of VRE, therefore, represents an immediate threat for [...]

Published
2005

37. A novel putative enterococcal pathogenicity island linked to the esp virulence gene of Enterococcus faecium and associated with epidemicity

Author

Leavis, Helen, Top, Janetta, Shankar, Nathan, Borgen, Katrine, Bonten, Marc, van Embden, Jan, and Willems, Rob J.L.

Subjects
Biological sciences
Abstract

Enterococcus faecalis harbors a virulence-associated surface protein encoded by the esp gene. This gene has been shown to be part of a 150-kb putative pathogenicity island. A gene similar to esp has recently been found in Enterococcus faecium isolates recovered from hospitalized patients. In the present study we analyzed the polymorphism in the esp gene of E. faecium, and we investigated the association of esp with neighboring chromosomal genes. The esp gene showed considerable sequence heterogeneity in the regions encoding the nonrepeat N- and C-terminal domains of the Esp protein as well as differences in the number of repeats. DNA sequencing of chromosomal regions flanking the esp gene of E. faecium revealed seven open reading frames, representing putative genes implicated in virulence, regulation of transcription, and antibiotic resistance. These flanking regions were invariably associated with the presence or absence of the esp gene in E. faecium, indicating that esp in E. faecium is part of a distinct genetic element. Because of the presence of virulence genes in this gene cluster, the lower G+C content relative to that of the genome, and the presence of esp in E. faecium isolates associated with nosocomial outbreaks and clinically documented infections, we conclude that this genetic element constitutes a putative pathogenicity island, the first one described in E. faecium. Except for the presence of esp and araC, this pathogenicity island is completely different from the esp-containing pathogenicity island previously disclosed in E. faecalis.

Published
2004

38. Epidemic and nonepidemic multidrug-resistant Enterococcus faecium

Author

Leavis, Helen L., Willems, Rob J.L., Top, Janetta, Spalburg, Emile, Mascini, Ellen M., Fluit, Ad C., Hoepelman, Andy, de Neeling, Albert J., and Bonten, Mark J.M.

Subjects
Drug resistance -- Physiological aspects, Drug resistance -- Genetic aspects, Enterococcal infections -- Genetic aspects, Enterococcal infections -- Development and progression, Enterococcal infections -- Care and treatment
Abstract

The epidemiology of vancomycin-resistant Enterococcus faecium (VREF) in Europe is characterized by a large community reservoir. In contrast, nosocomial outbreaks and infections (without a community reservoir) characterize VREF in the [...]

Published
2003

40. Contributors

Author

Alangaden, George J., primary, Aldape, Michael J., additional, Allardet-Servent, Jérôme, additional, Allen, Upton D., additional, Ammerlaan, Heidi S.M., additional, Angelakis, Emmanouil, additional, Artenstein, Andrew, additional, Asboe, David, additional, Asiedu, Kingsley B., additional, Atherton, John C., additional, Aw, Tar-Ching, additional, Baid-Agrawal, Seema, additional, Bailey, Robin, additional, Bandel, Christopher, additional, Barie, Philip S., additional, Barillo, David J., additional, Bart, Pierre-Alexandre, additional, Bayston, Roger, additional, Beard, C. Ben, additional, Beeching, Nick J., additional, Bégué, Rodolfo E., additional, Benhamou, Yves, additional, Benson, Constance A., additional, Berbari, Elie F., additional, Berendt, Anthony R., additional, Bhatta, Madhav P., additional, Bille, Jacques, additional, Bitnun, Ari, additional, Black, Finn T., additional, Blair, Iain, additional, Blanche, Stéphane, additional, Bleck, Thomas P., additional, Bleeker-Rovers, Chantal P., additional, Bleijenberg, Gijs, additional, Bloch, Karen C., additional, Bonten, Marc J.M., additional, Boucher, Charles A.B., additional, Bourayou, Rafik, additional, Bouza, Emilio S., additional, Bowie, William R., additional, Brause, Barry D., additional, Brisse, Sylvain, additional, Britton, Warwick, additional, Brook, Itzhak, additional, Brown, David W.G., additional, Brun-Buisson, Christian, additional, Brust, James C.M., additional, Bryant, Amy E., additional, Bryskier, André, additional, Buller, R. Mark L, additional, Bush, Karen, additional, Calandra, Thierry, additional, Cameron, D. William, additional, Caraël, Michel, additional, Carr, Michael J., additional, Casas, Inmaculada, additional, Chambers, Stephen T., additional, Chiller, Katarina G., additional, Chiller, Tom M., additional, Chiodini, Peter L., additional, Chopra, Ian, additional, Chu, Anthony C., additional, Chung, Kevin K., additional, Clark, Benjamin M., additional, Clumeck, Nathan, additional, Cockerell, Clay J., additional, Cohen, Jonathan, additional, Collinge, John, additional, Conlon, Christopher P., additional, Corey, G. Ralph, additional, Cross, Alan, additional, Cross, John H., additional, Currier, Judith, additional, Curtis, Carmel M., additional, Dallabetta, Gina, additional, Davidson, Robert N., additional, Davies, Jane, additional, Day, Jeremy, additional, Day, Nicholas P.J., additional, De Gascun, Cillian F., additional, de Wit, Stéphane, additional, Delmont, Jean, additional, Dennis, David T., additional, Diemert, David J., additional, Doganay, Mehmet, additional, Doherty, Tom, additional, Dolecek, Christiane, additional, Donati, Stéphane Y., additional, Dondorp, Arjen M., additional, Doudier, Barbara, additional, Drancourt, Michel, additional, Drekonja, Dimitri M., additional, Drew, Richard H., additional, Duker, Jay S., additional, Dummer, J. Stephen, additional, Edwards, Charles N., additional, Ekkelenkamp, Miquel B., additional, Enright, Mark C., additional, Epstein, Paul R., additional, Erard, Veronique, additional, Eziefula, Alice Chijioke, additional, Feinberg, Mark B., additional, Fenollar, Florence, additional, Fenwick, Alan, additional, Fernandez, Luis, additional, Fierer, Joshua, additional, Finch, Roger G., additional, Flexner, Charles W., additional, Fluit, Ad C., additional, Ford-Jones, Elizabeth Lee, additional, Fournier, Pierre-Edouard, additional, Fraser, Victoria, additional, French, Martyn A., additional, Friedland, Jon S., additional, Fritz, Joseph M., additional, Furuya, E. Yoko, additional, Gage, Kenneth L., additional, Garcia, Lynne S., additional, Gastañaduy, Arturo S., additional, Ghanem, Khalil G., additional, Giannella, Maddalena, additional, Glaser, Carol A., additional, Glesby, Marshall J., additional, Glover, Sarah, additional, Glupczynski, Youri, additional, Gnann, John W., additional, Goddard, Andrew F., additional, Goldstein, Ellie J.C., additional, González, Iveth J., additional, Gorbach, Sherwood L., additional, Gottstein, Bruno, additional, Gowda, Ravi, additional, Grabenstein, John D., additional, Grange, John M., additional, Green, Michael D., additional, Green, Stephen T., additional, Greenblatt, Danielle T., additional, Greenwood, Brian, additional, Gregson, Aric L., additional, Groll, Andreas H., additional, Gupta, Aditya K., additional, Gwee, Kok-Ann, additional, Hall, William, additional, Hammer, Scott M., additional, Handa, Sajeev, additional, Hanfelt-Goade, Diane, additional, Harari, Alexandre, additional, Harris, Marianne, additional, Hartman, Barry J., additional, Hay, Roderick J., additional, Henderson, David K., additional, Hensley, Lisa E., additional, Herbert, Luke, additional, Hill, David R., additional, Hills, Timothy J., additional, Hinze, John David, additional, Hirsch, Hans H., additional, Hirschel, Bernard, additional, Hoepelman, Andy I.M., additional, Holland, Steven M., additional, Horgan, Mary M., additional, Howe, Robin, additional, Hughes, James M., additional, Hull, Mark W., additional, Inderlied, Clark B., additional, Ison, Michael G., additional, Jenks, Peter J., additional, Johnson, James R., additional, Jones, Theodore, additional, Kanno, Mettassebia, additional, Kauffman, Carol, additional, Kelly, Patrick, additional, Kendler, Jason S., additional, Keynan, Yoav, additional, Khan, Ali S., additional, Kho, Grace T., additional, Kinghorn, George R., additional, Klapper, Paul E., additional, Kluytmans, Jan AJW, additional, Kok, Menno, additional, Koné-Paut, Isabelle, additional, Krieger, John N., additional, Kroes, Aloys C.M., additional, Kroon, Frank P., additional, Kubin, Christine J., additional, La Rosa, Alberto M., additional, Lalani, Tahaniyat, additional, Lalloo, David G., additional, Lambert, Harold, additional, Landraud, Luce, additional, Lawn, Stephen D., additional, Pharm, Phillipe Lehours, additional, Leone, Marc, additional, Levi, Itzchak, additional, Levitt, Alexandra M., additional, Lindquist, H. D. Alan, additional, Lloyd, Graham, additional, Looney, David J., additional, Lowy, Franklin D., additional, Luft, Benjamin J., additional, Lynn, William A., additional, Macielag, Mark J., additional, Mackowiak, Philip A., additional, MacPherson, Paul A., additional, Maghraoui-Slim, Valérie, additional, Main, Janice, additional, Mallet, Vincent, additional, Mangino, Julie E., additional, Manuel, Oriol, additional, Marchetti, Oscar, additional, Marks, Kristen, additional, Marr, Kieren A., additional, Martin, Claude, additional, Martín-Rabadán, Pablo, additional, Martinez, Augusto Julio, additional, Mascini, Ellen M., additional, Mayer, Kenneth H., additional, McCormick, Joseph B., additional, McGready, Rose, additional, McKendrick, Michael W., additional, Mead, Simon, additional, Mégraud, Francis, additional, Meheus, André Z., additional, Meintjes, Graeme, additional, Michaels, Marian G., additional, Miles, Michael, additional, Miller, Alastair, additional, Mimiaga, Matthew J., additional, Mingeot-Leclercq, Marie-Paule, additional, Mitchell, Thomas G., additional, Moise, Pamela A., additional, Montaner, Julio, additional, Moore, Caroline B., additional, Moreillon, Philippe, additional, Morgan-Capner, Peter, additional, Montessori, Valentina, additional, Moss, Peter, additional, Muñoz, Patricia, additional, Naber, Kurt G., additional, Nakhla, Sammy, additional, Narain, Jai P., additional, Nathwani, Dilip, additional, Newton, Paul, additional, Nguyen, Chinh, additional, Nicolle, Lindsay E., additional, Niederman, Michael S., additional, Noel, Gary J., additional, Norrby, S. Ragnar, additional, Nosten, François, additional, Notarangelo, Luigi Daniele, additional, Nyirjesy, Paul, additional, O'Connell, P. Ronan, additional, Odorico, Jon S., additional, Ong, Edmund L.C., additional, Opal, Steven M., additional, Ormerod, L. Peter, additional, Osmon, Douglas R., additional, Ottesen, Eric A., additional, Palacios, Gustavo, additional, Pantaleo, Giuseppe, additional, Papazian, Laurent, additional, Parola, Philippe, additional, Pascual, Manuel A., additional, Patrozou, Eleni, additional, Paya, Carlos, additional, Peacock, Sharon J., additional, Pechère, Jean-Claude, additional, Perkins, Mark D., additional, Peters, Barry, additional, Pfyffer, Gaby E., additional, Pham, Paul A., additional, Piot, Peter, additional, Placko-Parola, Geraldine, additional, Pol, Stainslas, additional, Posfay-Barbe, Klara M., additional, Powderly, William G., additional, Pozniak, Anton, additional, Prod'hom, Guy, additional, Quinn, Thomas C., additional, Rahn, Daniel W., additional, Rana, Aadia I., additional, Raoult, Didier, additional, Raz, Raul, additional, Razonable, Raymund, additional, Read, Robert C., additional, Reynolds, Stephen J., additional, Richardson, Malcolm D., additional, Robinson, Christopher C., additional, Rooijakkers, Suzan H.M., additional, Rosenbluth, Daniel, additional, Rosenzweig, Sergio D., additional, Rovery, Clarisse, additional, Rubin, Robert H., additional, Rubinovitch, Bina, additional, Rubins, Kathleen H., additional, Rubinstein, Ethan, additional, Ryan, Greg, additional, Ryder, Stephen, additional, Safren, Steven, additional, Sahasrabuddhe, Vikrant V., additional, Saikku, Pekka A.I., additional, Sakoulas, George, additional, Salazar, Juan Carlos, additional, Salvaggio, Michelle R., additional, Schaffer, Kirsten, additional, Schmitz, Franz-Josef, additional, Schooley, Robert T., additional, Schumacher, Richard-Fabian, additional, Scrimgeour, Euan M., additional, Seddon, James, additional, Seifert, Harald, additional, Serjeant, Graham R., additional, Sha, Beverly E., additional, Shah, Keerti V., additional, Shapiro, Daniel S., additional, Sheehan, Gerard, additional, Shoham, Shmuel, additional, Simmons, Cameron P., additional, Simonsen, Kari A., additional, Singh, Neeraj, additional, Slack, Mary P.E., additional, Sobel, Jack D., additional, Sopirala, Madhuri M., additional, Spacek, Lisa A., additional, Sriskandan, Shiranee, additional, Stanley, Samuel L., additional, Steckelberg, James M., additional, Stephenson, Iain, additional, Stevens, Dennis L., additional, Straus, Walter L., additional, Sturm, Willem, additional, Summerbell, Richard C., additional, Susa, Joseph S., additional, Tabrizi, Sarah J., additional, Tack, Marc A., additional, Taplitz, Randy, additional, Tebas, Pablo, additional, Temmerman, Marleen, additional, Thijsen, Steven F.T., additional, Thomas, Lora D., additional, Thomson, Gail, additional, Thwaites, Guy E., additional, Tirelli, Umberto, additional, Tolkoff-Rubin, Nina E., additional, Tønjum, Tone, additional, Torriani, Francesca J., additional, Townsend, Gregory C., additional, Masó, Gloria Trallero, additional, Tulkens, Paul M., additional, Tunkel, Allan R., additional, Vaccher, Emanuela, additional, Vallet-Pichard, Anaïs, additional, Van Bambeke, Françoise, additional, van de Beek, Diederik, additional, van der Meer, Jos W.M., additional, van Loon, Anton M., additional, van Putten, Jos, additional, Vaudaux, Bernard P., additional, Vermund, Sten H., additional, Verstraelen, Hans, additional, Verweij, Paul, additional, Viscidi, Raphael P., additional, Visvanathan, Kumar, additional, Visvesvara, Govinda S., additional, von Seidlein, Lorenz, additional, Wagenlehner, Florian M.E., additional, Wahl-Jensen, Victoria, additional, Walsh, Thomas J., additional, Warhurst, David C., additional, Warnock, David W., additional, Warrell, David A., additional, Warrell, Mary J., additional, Warris, Adilia, additional, Weber, Rainer, additional, Weidner, Wolfgang, additional, Weston, Vivienne C., additional, Whimbey, Estella, additional, Whitby, Michael, additional, White, Peter J., additional, Whitty, Christopher J.M., additional, Willems, Rob J.L., additional, Williams, Emrys, additional, Wilson, Cara, additional, Wilson, Mary E., additional, Winn, Richard E., additional, Winthrop, Kevin L., additional, Wiselka, Martin J., additional, Wisplinghoff, Hilmar, additional, Wolfe, Cameron R., additional, Wood, Robin, additional, Wright, Natalie, additional, Yankaskas, James R., additional, Zaidi, Najam A., additional, Zenilman, Jonathan M., additional, Zhang, Yaobi, additional, Zuckerman, Arie J., additional, Zuckerman, Jane Nicola, additional, and Zumla, Alimuddin, additional

Published
2010
Full Text
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43. In vitroevolution of colistin resistance in theKlebsiella pneumoniaecomplex follows multiple evolutionary trajectories with variable effects on fitness and virulence characteristics

Author

Janssen, Axel B., primary, Doorduijn, Dennis J., additional, Mills, Grant, additional, Rogers, Malbert R.C., additional, Bonten, Marc J.M., additional, Rooijakkers, Suzan H.M., additional, Willems, Rob J.L., additional, Bengoechea, Jose A., additional, and Schaik, Willem van, additional

Published
2020
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47. The complement system facilitates clearance of Enterococcus faecium during murine peritonitis

Author

Leendertse, Masja, Willems, Rob J.L., Flierman, Roelof, de Vos, Alex F., Bonten, Marc J.M., and van der Poll, Tom

Subjects
Complement system -- Physiological aspects, Complement system -- Research, Enterococcal infections -- Patient outcomes, Enterococcal infections -- Research, Peritonitis -- Patient outcomes, Peritonitis -- Research, Health
Published
2010

48. Enterococcal surface protein transiently aggravates Enterococcus faecium-induced urinary tract infection in mice

Author

Leendertse, Masja, Heikens, Esther, Wijnands, Lucas M., van Luit-Asbroek, Miranda, Teske, Gwendoline J.D., Roelofs, Joris J.T.H., Bonten, Marc J.M., van der poll, Tom, and Willems, Rob J.L.

Subjects
Urinary tract infections -- Development and progression, Urinary tract infections -- Research, Enterococcus -- Physiological aspects, Enterococcus -- Research, Virulence (Microbiology) -- Research, Rats as laboratory animals -- Research, Health
Published
2009

49. Intestinal enterococcus faecium colonization improves host defense during polymicrobial peritonitis

Author

Leendertse, Masja, Willems, Rob J.L., Oei, G. Anneke, Florquin, Sandrine, Bonten, Marc J.M., and van der Poll, Tom

Subjects
Enterococcal infections -- Research, Enterococcal infections -- Genetic aspects, Peritonitis -- Research, Peritonitis -- Physiological aspects, Colorectal diseases -- Research, Colorectal diseases -- Physiological aspects, Gastrointestinal diseases -- Research, Gastrointestinal diseases -- Physiological aspects, Health
Published
2009

50. Corrigendum : Mlplasmids: A user-friendly tool to predict plasmid-and chromosome-derived sequences for single species, (Microbial Genomics), (2018), 4, 10.1099/mgen.0.000224

Author

Arredondo-Alonso, Sergio, Rogers, Malbert R.C., Braat, Johanna C., Verschuuren, Tess D., Top, Janetta, Corander, Jukka, Willems, Rob J.L., and Schürch, Anita C.

Subjects
Medicine(all), Published Erratum, Journal Article
Abstract

There was an error in the Funding information section in the published article. The Funding information section should include the following sentence: ‘This study was supported by the Joint Programming Initiative in Antimicrobial Resistance (JPIAMR Third call, STARCS, JPIAMR2016-AC16/00039 to SA, RJLW).’ The author apologizes for any inconvenience caused.

Published
2019

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