48 results on '"Wilhelm JM"'
Search Results
2. Syndrome des ongles jaunes. À propos de deux cas. Revue de la littérature
- Author
-
Kianzowa, M, primary, Saraceni, O, additional, Wilhelm, JM, additional, and Kieffer, P, additional
- Published
- 1994
- Full Text
- View/download PDF
3. Prevalence of Temporomandibular Disorders and Anxiety State Levels in Ballet Dancers A Cross-Sectional Study.
- Author
-
Hilgenberg-Sydney PB, Wilhelm JM, Pimentel G, Petterle R, and Bonotto D
- Subjects
- Adolescent, Adult, Age Factors, Anxiety psychology, Arthralgia psychology, Comorbidity, Cross-Sectional Studies, Dancing psychology, Female, Headache epidemiology, Humans, Male, Prevalence, Temporomandibular Joint Disorders psychology, Young Adult, Anxiety epidemiology, Dancing statistics & numerical data, Health Status, Temporomandibular Joint Disorders epidemiology
- Abstract
This study evaluated the prevalence of temporomandibular disorders (TMDs) in ballet dancers and explored their association with levels of anxiety. Participants were 51 male and female ballet dancers with a mean age of 31.5 ± 12.6 years. The diagnosis of TMDs was made in accordance with Research Diagnostic Criteria for Temporomandibular Joint Disorders (RDC/TMD). All subjects completed the state part of an anxiety questionnaire (STAI). Data were gathered and analyzed using the R statistical software (version: 3.4.0.) with level of significance set at 5%. The prevalence of all TMDs in the sample was 78%; that is, of the 51 participants, 39 had at least one positive diagnosis. The two most prevalence diagnoses were disk displacement with reduction and arthralgia. Individuals with TMD had a mean anxiety score of 42.55 ± 9.92 on the STAI, whereas for those without TMD it was 44.27 ± 11.89, with no statistically significant difference (p = 0.53). It is concluded that the general prevalence of TMDs in ballet dancer is higher than in the population at large. Disk displacement with reduction and arthralgia are the most common TMDs, and the presence of TMDs does not seem to affect anxiety state levels in this population.
- Published
- 2020
- Full Text
- View/download PDF
4. Liver adenomatosis in patients with hepatocyte nuclear factor-1 alpha maturity onset diabetes of the young (HNF1A-MODY): Clinical, radiological and pathological characteristics in a French series.
- Author
-
Haddouche A, Bellanne-Chantelot C, Rod A, Fournier L, Chiche L, Gautier JF, Timsit J, Laboureau S, Chaillous L, Valero R, Larger E, Jeandidier N, Wilhelm JM, Popelier M, Guillausseau PJ, Thivolet C, Lecomte P, Benhamou PY, and Reznik Y
- Subjects
- Adenoma diagnostic imaging, Adenoma pathology, Adolescent, Adult, Child, Cohort Studies, Diabetes Mellitus, Type 2 diagnostic imaging, Diabetes Mellitus, Type 2 pathology, Diagnostic Imaging methods, Family Health, Female, France, Hepatocyte Nuclear Factor 1-alpha metabolism, Humans, Liver Neoplasms diagnostic imaging, Liver Neoplasms pathology, Male, Middle Aged, Young Adult, Adenoma genetics, Diabetes Mellitus, Type 2 genetics, Hepatocyte Nuclear Factor 1-alpha genetics, Liver Neoplasms genetics, Mutation
- Abstract
Background: Liver adenomatosis (LA) is a rare disease resulting from biallelic inactivation of the hepatocyte nuclear factor-1 alpha (HNF1A) gene, which induces the proliferation of adenoma cells in liver parenchyma. Liver adenomatosis has only been documented in case reports from patients carrying a HNF1A germline mutation. We have evaluated the frequency of LA among a large cohort of patients with HNF1A-maturity onset diabetes of the young (MODY), previously termed "MODY3," and herein describe its clinical, radiological, and pathological characteristics., Methods: In all, 137 HNF1A-MODY subjects from 74 families were screened by liver ultrasonography in 13 centers, and 15 additional cases of LA were later included in the series. Liver adenomatosis was confirmed by liver computed tomography, magnetic resonance imaging (MRI), and/or histopathology., Results: Among 137 carriers of an HNF1A mutation, 9 patients (6.5%) from seven families were diagnosed with LA. Diabetes mellitus was present in 87.5% of patients with LA. In 25% of patients, LA was diagnosed due to intra-abdominal or intratumoral bleeding. Liver biochemistry was near normal in all patients. Liver imaging showed adenomas of various sizes and numbers. On MRI, most nodules had the radiological characteristics of steatotic adenomas. Histopathological confirmation of LA was available in 13 cases, and these adenomas were mostly steatotic. Surgery was initially performed in 37.5% of patients, and liver disease progression was observed in 30%. No disease progression was observed in 14 pregnancies., Conclusions: The frequency of LA in a cohort of screened HNF1A-MODY patients and the high incidence of LA progression and/or hemorrhage warrants systematic screening for liver adenomatosis in HNF1A-MODY families., (© 2019 Ruijin Hospital, Shanghai Jiaotong University School of Medicine and John Wiley & Sons Australia, Ltd.)
- Published
- 2020
- Full Text
- View/download PDF
5. Obstructive sleep apnoea in people with Type 1 diabetes: prevalence and association with micro- and macrovascular complications.
- Author
-
Manin G, Pons A, Baltzinger P, Moreau F, Iamandi C, Wilhelm JM, Lenoble P, Kessler L, and Kessler R
- Subjects
- Adult, Body Mass Index, Cardiovascular Diseases etiology, Cross-Sectional Studies, Diabetes Mellitus, Type 1 epidemiology, Diabetic Angiopathies etiology, Diabetic Neuropathies etiology, Female, Humans, Male, Middle Aged, Polysomnography, Prevalence, Risk Factors, Sleep Apnea, Obstructive etiology, Cardiovascular Diseases epidemiology, Diabetes Mellitus, Type 1 complications, Diabetic Angiopathies epidemiology, Diabetic Neuropathies epidemiology, Sleep Apnea, Obstructive epidemiology
- Abstract
Aims: Few reports have assessed the relationship between Type 1 diabetes and sleep disorders. The purposes of our study were to determine the prevalence of obstructive sleep apnoea in Type 1 diabetes and to compare the clinical profile of people with Type 1 diabetes with or without obstructive sleep apnoea., Methods: In this cross sectional study of 67 consecutive people with Type 1 diabetes, we performed polysomnography as part of their yearly check-ups., Results: In our cohort, with a mean BMI of 25.8 ± 4.7 kg/m(2), the prevalence of obstructive sleep apnoea [apnoea-hypopnoea index (AHI) > 10/h] was 46%. Severe obstructive sleep apnoea (AHI ≥ 30/h) was present in 19% of the patients. We found no significant differences in age, sex, body mass index, HbA1c or Epworth sleepiness scale score between people with or without obstructive sleep apnoea. People with obstructive sleep apnoea had a longer course of diabetes mellitus (P < 0.01) and a higher prevalence of retinopathy (P < 0.01), neuropathy (P = 0.05), cardiovascular disease (P < 0.01) and hypertension (P < 0.01). The occurrence of macrovascular complications was independently associated with the presence of OSA [odds ratio (OR) 8.28; 95% confidence interval (CI), 1.56-43.97; P = 0.013] and the duration of diabetes (OR 1.08; 95% CI, 1.02-1.15; P = 0.01). Moreover, retinopathy was independently associated with OSA (OR 4.54; 95% CI, 1.09-18.82; P = 0.04) and the duration of diabetes (OR 1.09; 95% CI, 1.04-1.15; P = 0.001)., Conclusions: The prevalence of obstructive sleep apnoea was high in people with Type 1 diabetes. Obstructive sleep apnoea was independently associated with macrovascular complications and retinopathy. Obesity and excessive daytime sleepiness were uncommon in this population., (© 2014 The Authors. Diabetic Medicine © 2014 Diabetes UK.)
- Published
- 2015
- Full Text
- View/download PDF
6. [Recurrent nerve palsy due to Lyme disease: report of two cases].
- Author
-
Martzolff L, Bouhala M, Dukic R, Saraceni O, Wilhelm JM, Bombaron P, and Kieffer P
- Subjects
- Aged, 80 and over, Anti-Bacterial Agents therapeutic use, Female, Humans, Lyme Disease complications, Lyme Disease diagnosis, Lyme Neuroborreliosis drug therapy, Male, Middle Aged, Sensitivity and Specificity, Serologic Tests, Treatment Outcome, Vocal Cord Paralysis drug therapy, Borrelia burgdorferi isolation & purification, Lyme Neuroborreliosis complications, Lyme Neuroborreliosis diagnosis, Vocal Cord Paralysis diagnosis, Vocal Cord Paralysis microbiology
- Abstract
Introduction: Neuroborreliosis can be a difficult diagnosis which requires epidemiologic, clinical and biologic arguments., Case Reports: We report two patients who presented with a recurrent laryngeal nerve palsy with positive Lyme serology and favorable outcome after antibiotic therapy. In one case, a lymphocytic meningitis with intrathecal production of specific antibodies was evidenced., Conclusion: Recurrent laryngeal nerve palsy is an uncommon manifestation of neuroborreliosis. Lyme serology is an important tool when neurologic disorder occurs because of an atypical course of Lyme disease., (2009 Société nationale française de médecine interne (SNFMI). Published by Elsevier SAS. All rights reserved.)
- Published
- 2010
- Full Text
- View/download PDF
7. A novel variation in the Twinkle linker region causing late-onset dementia.
- Author
-
Echaniz-Laguna A, Chanson JB, Wilhelm JM, Sellal F, Mayençon M, Mohr M, Tranchant C, and Mousson de Camaret B
- Subjects
- Adult, Age of Onset, Aged, Aged, 80 and over, Dementia diagnosis, Female, Genes, Dominant, Humans, Male, Mitochondrial Diseases genetics, Mitochondrial Proteins, Muscular Diseases diagnosis, Muscular Diseases genetics, Neurophysiology methods, Ophthalmoplegia, Chronic Progressive External diagnosis, Pedigree, DNA Helicases genetics, DNA, Mitochondrial genetics, Dementia genetics, Ophthalmoplegia, Chronic Progressive External genetics
- Abstract
Variations in the mitochondrial helicase Twinkle (PEO1) gene are usually associated with autosomal dominant chronic progressive external ophthalmoplegia (PEO). We describe five patients from two unrelated Alsatian families with the new R374W variation in the Twinkle linker region who progressively developed an autosomal dominant multisystem disorder with PEO, hearing loss, myopathy, dysphagia, dysphonia, sensory neuropathy, and late-onset dementia resembling Alzheimer's disease. These observations demonstrate that Twinkle variations in the linker domain alter cerebral function and further implicate disrupted mitochondrial DNA integrity in the pathogenesis of dementia.
- Published
- 2010
- Full Text
- View/download PDF
8. [Systematic determination of diabetic microangiopathy combined with therapeutic education].
- Author
-
Wilhelm JM, Weigel C, Tolosa G, Demure A, Demark S, Faller M, Haennig A, Lichtlé AL, and Muller R
- Subjects
- Blood Glucose Self-Monitoring, Diabetic Angiopathies prevention & control, Diabetic Nephropathies prevention & control, Diet, Diabetic, Humans, Diabetic Angiopathies diagnosis, Diabetic Angiopathies rehabilitation, Patient Education as Topic
- Published
- 2008
9. [A young woman with granulomatous mastitis: a corynebacteria may be involved in the pathogenesis of these disease].
- Author
-
Kieffer P, Dukic R, Hueber M, Kieffer C, Bouhala M, Riegel P, and Wilhelm JM
- Subjects
- Adult, Amoxicillin therapeutic use, Anti-Bacterial Agents therapeutic use, Corynebacterium classification, Corynebacterium isolation & purification, Diagnosis, Differential, Female, Glucocorticoids therapeutic use, Granuloma, Plasma Cell diagnosis, Humans, Immunosuppressive Agents therapeutic use, Mastitis diagnosis, Methotrexate therapeutic use, Prednisone therapeutic use, Punctures, Recurrence, Corynebacterium Infections diagnosis, Granuloma, Plasma Cell microbiology, Mastitis microbiology
- Abstract
Introduction: The granulomatous mastitis is an inflammatory pseudotumor of the breast of which evolution benign but likely to generate important morphological after-effects among young women. This anatomoclinic entity of dubious etiology until these last years poses a problem of differential diagnosis with other etiologies of granulomatosis and especially with inflammatory carcinoma of the breast. The infectious theory is actually based on solid arguments and mainly explains the physiopathology of this affection., Interpretation: A 26 years old young woman developed an inflammatory tumor of the left breast of which the catch of load by surgery and an antibiotherapy had shown trailing local continuations and of the esthetic after-effects. One year later, a very inflammatory repetition on the level of the right breast was dealt with in a different way: by steroids and immunomodulating drugs associated with iterative punctures with the purulent collections, the objective being to be less dilapidating that left side. The initial answer was rather favorable and encouraging but the purulent reappearance bulky granulomas with sinus way made reconsider the therapeutic attitude and antibiotics were undertaken after description of a lipophilic corynebactery in the material of puncture (Corynebacteria kroppenstedtii). The effectiveness of the amoxicilline introduced on the data of the antibiogram was undeniable., Conclusion: This observation illustrates the therapeutic and diagnostic difficulties of an exceptional affection. Potentially accessible to antibiotics it generally requires a joint surgical assumption of responsibility, at the same time to ensure the histological diagnosis but also with a therapeutic aim. The interest of steroids and the immunomodulation by methotrexate is debatable, these treatments cannot however be conceived without antibiotherapy and sometimes surgery.
- Published
- 2006
- Full Text
- View/download PDF
10. Synthesis and structure-activity relationship of novel 6-aryl-1,4-dihydrobenzo[d][1,3]oxazine-2-thiones as progesterone receptor modulators leading to the potent and selective nonsteroidal progesterone receptor agonist tanaproget.
- Author
-
Fensome A, Bender R, Chopra R, Cohen J, Collins MA, Hudak V, Malakian K, Lockhead S, Olland A, Svenson K, Terefenko EA, Unwalla RJ, Wilhelm JM, Wolfrom S, Zhu Y, Zhang Z, Zhang P, Winneker RC, and Wrobel J
- Subjects
- Alkaline Phosphatase metabolism, Animals, Area Under Curve, Benzoxazines chemistry, Benzoxazines pharmacology, Binding, Competitive, Cell Line, Tumor, Contraceptive Agents, Female chemical synthesis, Contraceptive Agents, Female chemistry, Contraceptive Agents, Female pharmacology, Decidua drug effects, Decidua metabolism, Female, Half-Life, Humans, In Vitro Techniques, Ligands, Molecular Structure, Oxazines chemistry, Oxazines pharmacology, Protein Structure, Tertiary, Pyrroles chemistry, Pyrroles pharmacology, Rats, Rats, Sprague-Dawley, Receptors, Progesterone antagonists & inhibitors, Receptors, Progesterone chemistry, Structure-Activity Relationship, Thiones chemistry, Thiones pharmacology, Benzoxazines chemical synthesis, Oxazines chemical synthesis, Pyrroles chemical synthesis, Receptors, Progesterone agonists, Thiones chemical synthesis
- Abstract
Tanaproget represents a potential first-in-class nonsteroidal PR agonist for contraception with improved safety and side effect profiles versus currently available steroidal oral contraceptives. Additional SAR, biological activity, and structural information from a tanaproget/hPR-LBD (hPR-LBD = human progesterone receptor ligand binding domain) cocrystal structure will also be presented.
- Published
- 2005
- Full Text
- View/download PDF
11. [Minocycline-induced lymphomatoid papulosis].
- Author
-
Dukic R, Derragui A, Geiss S, Wilhelm JM, Thannberger P, Colson A, and Kieffer P
- Subjects
- Acne Vulgaris drug therapy, Adult, Anti-Bacterial Agents therapeutic use, Humans, Male, Minocycline therapeutic use, Anti-Bacterial Agents adverse effects, Lymphomatoid Papulosis chemically induced, Minocycline adverse effects
- Published
- 2004
- Full Text
- View/download PDF
12. Clinical spectrum associated with hepatocyte nuclear factor-1beta mutations.
- Author
-
Bellanné-Chantelot C, Chauveau D, Gautier JF, Dubois-Laforgue D, Clauin S, Beaufils S, Wilhelm JM, Boitard C, Noël LH, Velho G, and Timsit J
- Subjects
- Adult, Atrophy, Diabetes Mellitus, Type 2 pathology, Diabetic Nephropathies genetics, Diabetic Nephropathies pathology, Exocrine Pancreatic Insufficiency complications, Exocrine Pancreatic Insufficiency pathology, Genes, Dominant, Genitalia abnormalities, Hepatocyte Nuclear Factor 1, Hepatocyte Nuclear Factor 1-alpha, Hepatocyte Nuclear Factor 1-beta, Humans, Kidney abnormalities, Kidney pathology, Pancreas pathology, Phenotype, DNA-Binding Proteins genetics, Diabetes Mellitus, Type 2 genetics, Mutation, Nuclear Proteins, Transcription Factors genetics
- Abstract
Background: Maturity-onset diabetes of the young type 5 (MODY5), a type of dominantly inherited diabetes mellitus and nephropathy, has been associated with mutations of the hepatocyte nuclear factor-1beta (HNF-1beta) gene, mostly generating truncated protein. Various phenotypes, including urogenital malformations, are related to HNF-1beta mutations., Objective: To describe clinical and genetic findings in 13 patients with 8 novel HNF-1beta mutations., Design: Multicenter, descriptive study., Setting: 2 departments of diabetes, 1 department of internal medicine, and 1 department of nephrology., Participants: 8 probands with diabetes diagnosed before 40 years of age and nondiabetic kidney disease who were selected independent of their family history of diabetes, and 5 offspring., Measurements: Characteristics of diabetes, renal function and structure, genital tract abnormalities, pancreas structure, insulin secretion, exocrine pancreas function, and liver test results., Results: All mutations, including 5 missense changes, were found in the DNA-binding domain. Cosegregation of the mutation and MODY5 phenotype was observed in 4 families. Occurrence of a de novo mutation was demonstrated in 2 families. Diabetes was present in 10 of 13 mutation carriers. It was clinically overt in 5 participants and found by screening at age 19 to 38 years in 5 participants. Pancreas atrophy was observed in 5 of 6 probands, and pancreas exocrine insufficiency was observed in 6 of 7 probands. Renal involvement, consisting of structural changes and slowly progressive renal failure, was recognized in 9 patients at 18 to 41 years of age. Dysplastic kidneys were found by ultrasonography in 3 fetuses who subsequently showed transient neonatal renal failure. Genital tract abnormalities were present in 5 probands and liver enzyme levels were abnormal in 11 of 13 patients., Limitations: Since the study was small and not population-based, it could not estimate the prevalence of MODY5. Other phenotypes might be associated with HNF-1beta mutations., Conclusions: Maturity-onset diabetes of the young type 5 encompasses a wide clinical spectrum. Analysis for mutations of HNF-1beta is warranted, even without a family history of diabetes, in nonobese patients with diabetes and slowly progressive nondiabetic nephropathy, particularly when pancreatic atrophy or genital abnormalities are present.
- Published
- 2004
- Full Text
- View/download PDF
13. [Paraneoplastic adult Still's disease].
- Author
-
Kianzowa M, Dukic R, Derragui A, Wilhelm JM, Saraceni O, and Kieffer P
- Subjects
- Female, Humans, Middle Aged, Still's Disease, Adult-Onset etiology, Breast Diseases complications, Paraneoplastic Syndromes, Still's Disease, Adult-Onset pathology
- Published
- 2002
- Full Text
- View/download PDF
14. [A plea for a chronobiological approach to diabetes mellitus].
- Author
-
Wilhelm JM, Thannberger P, Dukic R, Derragui A, Saraceni O, and Kieffer P
- Subjects
- Blood Glucose analysis, Chronobiology Phenomena, Diabetes Mellitus, Type 2 blood, Humans, Lipids blood, Diabetes Mellitus, Type 2 physiopathology
- Abstract
Diabetes mellitus is, in most patients, a multi-metabolic condition disease under fixed standardized conditions. We develop here the advantages of a chronobiological approach, exploring nycthemeral variations in blood pressure, variations in insulin resistance, postprandial changes in blood glucose and postabsorptive variations in blood lipids. Such information can help improve our understanding of the disease, better identify risk and prognosis, and enlighten therapeutic options.
- Published
- 2002
15. [Von Recklinghausen's disease associated with pancreatic somatostatinoma].
- Author
-
Thannberger P, Wilhelm JM, Derragui A, Saraceni O, and Kieffer P
- Subjects
- Adult, Humans, Male, Neurofibromatosis 1 diagnosis, Pancreas pathology, Pancreatic Neoplasms diagnosis, Pancreatic Neoplasms pathology, Pancreatic Neoplasms surgery, Somatostatin blood, Somatostatinoma diagnosis, Somatostatinoma pathology, Somatostatinoma surgery, Neurofibromatosis 1 complications, Pancreatic Neoplasms complications, Somatostatinoma complications
- Abstract
Introduction: Pancreatic somatostatinoma is a rare entity and its association with Von Recklinghausen's disease has only been described on two occasions. We report a new observation, with clinical and evolving status differing from those described in isolated cases of somatostatinoma., Observation: A 28 year-old man presenting with familial Von Recklinghausen's disease, had suffered for 10 years from paroxysmic abdominal pain. He was hospitalized for intense pain. Imaging revealed a retroperineal tumoral formation. Following duodenopancreatectomy, somatostatinoma was diagnosed. Diffuse metastatic miliary was revealed and multiple glandular metastases., Discussion: Other than the rarity of the morbid association (pancreatic somatostatinoma and Von Recklinghausen's disease) described, this case is particular in that the patient was very young, symptomatology was unapparent and tumoral evolution was minimal.
- Published
- 2001
16. Multiple organ dysfunction dramatically improving with the infusion of vitamin C: more support for the persistence of scurvy in our "welfare" society.
- Author
-
Kieffer P, Thannberger P, Wilhelm JM, Kieffer C, and Schneider F
- Subjects
- Aged, Diagnosis, Differential, Hemodynamics drug effects, Humans, Male, Multiple Organ Failure diagnosis, Multiple Organ Failure drug therapy, Scurvy diagnosis, Ascorbic Acid therapeutic use, Scurvy drug therapy, Scurvy physiopathology
- Published
- 2001
- Full Text
- View/download PDF
17. [Acute disulfiram intoxication with severe neurological sequaelae and presenting misleading psychiatric deficiency].
- Author
-
Thannberger P, Wilhelm JM, Saraceni O, Derragui A, Dietemann JL, and Kieffer P
- Subjects
- Adult, Brain diagnostic imaging, Brain pathology, Diagnosis, Differential, Female, Humans, Magnetic Resonance Imaging, Poisoning physiopathology, Poisoning psychology, Suicide, Attempted, Tomography, X-Ray Computed, Alcohol Deterrents poisoning, Disulfiram poisoning, Poisoning diagnosis
- Abstract
Disulfiram self poisoning is exceptional. The authors report on the case of such an intoxication related to the ingestion of a potentially lethal dose of this drug (30 g) and draw the attention on the following points: 1) The initial signs may be misleading because they include both psychiatrics and neurological signs such as phonation abnormalities, myoclonias and tetraparesia. 2) The evolution is unforseeable with the possible occurrence of severe psychological and motricity sequaelae, associated with bilateral and symetric injuries of the putamen, the palladium and the basal nuclei on CT-scan (or MRI). The pathophysiologic al mechanisms of theses signs are discussed, and the need for disulfiram in the care of alcoholic patients seeking for withdrawal as well.
- Published
- 2000
18. [Budd-Chiari syndrome with inferior vena cava stenosis].
- Author
-
Thannberger P, Ruxer S, Derragui A, Saraceni O, Wilhelm JM, Weisse D, and Kieffer P
- Subjects
- Angioplasty, Balloon, Budd-Chiari Syndrome diagnosis, Budd-Chiari Syndrome therapy, Constriction, Pathologic complications, Constriction, Pathologic diagnosis, Constriction, Pathologic therapy, Female, Humans, Middle Aged, Stents, Budd-Chiari Syndrome etiology, Vena Cava, Inferior
- Abstract
Background: Stenosis of the inferior vena cava is a rare cause of Budd-Chiari's syndrome. We report on such a case related to a specific cause with a dramatic improvement after endovascular treatment., Case Report: A 53-year old woman was admitted to hospital because she demonstrated circulatory collapse, inferior limbs edema and post-prandial epigastralgia. These signs were related to the occurrence of a stenosis of the sus-hepatic segment of the inferior vena cava related to the presence of the distal part of a catheter. An angioplasty with the insertion of a stent was followed by a total recovery with a one-year survey., Conclusion: Angioplasty with the insertion of a stent should be considered in a Budd-Chiari syndrome related to the stenosis of the inferior vena cava. This option should be evaluated with reference to classic surgical techniques.
- Published
- 2000
19. [Thrombotic manifestations revealing a digestive tract disease].
- Author
-
Thannberger P, Wilhelm JM, Ruxer S, Derragui A, Saraceni O, and Kieffer P
- Subjects
- Aged, Digestive System Diseases complications, Digestive System Diseases diagnosis, Female, Humans, Intestinal Absorption, Malabsorption Syndromes diagnosis, Male, Middle Aged, Phlebitis etiology, Vitamin B 12 Deficiency diagnosis, Malabsorption Syndromes complications, Myocardial Infarction etiology, Venous Thrombosis etiology, Vitamin B 12 Deficiency complications
- Published
- 2000
- Full Text
- View/download PDF
20. [Interstitial pneumopathy 2 months after discontinuing treatment with amiodarone].
- Author
-
Wilhelm JM, Thannberger P, Derragui A, Ruxer S, Saraceni O, and Kieffer P
- Subjects
- Aged, Amiodarone therapeutic use, Humans, Long-Term Care, Lung Diseases, Interstitial diagnosis, Male, Vasodilator Agents therapeutic use, Amiodarone adverse effects, Lung Diseases, Interstitial chemically induced, Myocardial Ischemia drug therapy, Substance Withdrawal Syndrome diagnosis, Vasodilator Agents adverse effects
- Published
- 1999
21. [Campylobacter fetus meningitis in adults].
- Author
-
Wilhelm JM, Saraceni O, Penner MF, Trévoux A, and Kieffer P
- Subjects
- Aged, Aged, 80 and over, Anti-Bacterial Agents, Drug Therapy, Combination therapeutic use, Humans, Male, Campylobacter Infections cerebrospinal fluid, Campylobacter Infections drug therapy, Campylobacter fetus, Meningitis, Bacterial cerebrospinal fluid, Meningitis, Bacterial drug therapy
- Abstract
Campylobacter fetus is an uncommon cause of meningitis in the adult. We report a case observed in an 84-year-old man with alcoholic cirrhosis. The patient presented fever, jaundice and a state of mental confusion. Blood and cerebrospinal fluid cultures identified Campylobacter fetus sensitive to several antibiotics. Ciprofloxacine-ceftriaxone combination replaced the antibiotics prescribed empirically prior to identification and led to regression of the fever and normal mental status within 4 days. Spinal tap on day 7 showed 20 white cells, 85% lymphocytes and normal protein level. Unfortunately, the patient later developed edema and ascitis with major jaundice. Oligo-anuria could not be controlled and the patient died two weeks after admission. Campylobacter fetus meningitis is predominantly seen in men, mean age of onset 50 years. Clinical signs are not specific and diagnosis can only be obtained on the basis of cerebrospinal fluid results. Adapted antibiotics are required.
- Published
- 1996
22. [Amiodarone-induced hypothyroidism followed by hyperthyroidism. Apropos of 2 new cases].
- Author
-
Wilhelm JM, Saraceni O, and Kieffer P
- Subjects
- Aged, Female, Humans, Hyperthyroidism physiopathology, Hypothyroidism physiopathology, Male, Middle Aged, Amiodarone adverse effects, Anti-Arrhythmia Agents adverse effects, Hyperthyroidism chemically induced, Hypothyroidism chemically induced
- Abstract
Amiodarone, an iodine-rich benzofuranic derivative, may often induce hyperthyroidism and hypothyroidism. We report two cases of complex dysthyroidism, in which hypothyroidism and then hyperthyroidism alternated: we recall five former observations found in the literature. Their nosologic position and pathophysiologic mechanisms are discussed.
- Published
- 1996
23. [Yellow nail syndrome. Apropos of 2 cases. Review of the literature].
- Author
-
Kianzowa M, Saraceni O, Wilhelm JM, and Kieffer P
- Subjects
- Aged, Aged, 80 and over, Female, Humans, Male, Middle Aged, Syndrome, Breast Neoplasms complications, Laryngeal Neoplasms complications, Lymphedema complications, Nail Diseases etiology
- Abstract
The yellow nail syndrome is a clinical syndrome without specific biological parameters. It is characterized by the following threesome of symptoms: nail abnormalities, primary lymphedema, and pleuropulmonary manifestations. In most cases lymphography shows abnormalities of the lymphatic vessels and spirometry shows a restrictive defect and rarely obstructive disorders. Pathogenesis of this syndrome is still unknown. It is, however, remarkable that the yellow nail syndrome may occur in association with other diseases, the most frequent of which are cancer affections and immune disorders. We report two cases of the yellow nail syndrome which were associated with cancer (neoplasm of the larynx and breast).
- Published
- 1994
- Full Text
- View/download PDF
24. [Lyme disease disclosed by disabling amyotrophy].
- Author
-
Kianzowa M, Saraceni O, Wilhelm JM, and Kieffer P
- Subjects
- Aged, Anti-Bacterial Agents therapeutic use, Humans, Immunohistochemistry, Lyme Disease diagnosis, Lyme Disease drug therapy, Lyme Disease immunology, Male, Muscular Dystrophies drug therapy, Muscular Dystrophies immunology, Pain etiology, Lyme Disease complications, Muscular Dystrophies etiology
- Abstract
The Lyme disease, which was first found in Lyme, USA, in 1975 is an infectious multi-system disorder whose agent, the Borrelia Burgdorferi, was identified in 1983. Various disease manifestations have been reported since them. We report a case of Lyme disease manifestation in the muscle revealed by serious amyotrophy in the scapular muscles, in the neck and in the trunk with scapula alata accompanied by noctural lumbago which is resistant to non steroïd anti-inflammatory drugs. Electromyography showed patterns of the myogenic type in the serrate anterior and infraspinous muscles on both sides and patterns of the neurogenic peripheral type in the lower extremities. The histological, immunohistochemical and electron-microscopal studies of the muscle biopsy have been without any pathological result. Creatine kinase and aldolase proved normal. Serological antibodies were not significantly increased. A positive diagnosis was obtained by lumbar puncture which showed lymphocytic meningitis, by demonstrating specific antibodies in the cerebrospinal fluid and by Western Blot antibodies analysis. Efficacy of the antibiotic therapy has resulted in a rapid regression of the symptoms, which is a further confirmation of the previous diagnosis.
- Published
- 1993
- Full Text
- View/download PDF
25. Serological evidence for culture-negative listeriosis of central nervous system.
- Author
-
Gaillard JL, Beretti JL, Boulot-Tolle M, Wilhelm JM, Bertrand JL, Herbelleau T, and Berche P
- Subjects
- Central Nervous System Diseases diagnosis, Female, Humans, Male, Middle Aged, Serologic Tests, Central Nervous System Diseases microbiology, Listeriosis diagnosis
- Published
- 1992
- Full Text
- View/download PDF
26. Post-translational cleavage of mucocyst precursors in Tetrahymena.
- Author
-
Collins T and Wilhelm JM
- Subjects
- Animals, Dibucaine pharmacology, Kinetics, Microscopy, Electron, Molecular Weight, Peptide Biosynthesis, Peptide Fragments analysis, Tetrahymena pyriformis drug effects, Tetrahymena pyriformis ultrastructure, Mucus metabolism, Protein Biosynthesis, Tetrahymena pyriformis physiology
- Abstract
Pulse-chase experiments utilizing intact Tetrahymena revealed that at least six proteins (molecular weights, 61,000, 56,000, 51,000, 48,000, 42,000, and 38,000) were unstable and underwent proteolytic cleavage during the first 20 min of the chase period. At least 9 product polypeptides (molecular weights, 45,000, 41,000, 25,000, 21,000, 20,000, 18,000, 17,000, 16,000, and 15,000) appeared during the chase. The proposal that the product polypeptides were actually mucocyst constituents was supported by a variety of observations. First, treatment of whole cells in a complete media with dibucaine caused precursor cleavage and product accumulation with kinetics that were consistent with previous morphologic observations on mucocyst formation. Second, the product polypeptides were enriched in a cell fraction containing just cortical components and amorphous material consistent with aggregated mucus. Third, the labeled product polypeptides in the cortex comigrated with partially purified labeled mucus obtained by dibucaine treatment of whole cells. Fourth, one-dimensional peptide mapping of the 45-kilodalton product confirmed that the post-translationally derived product in whole cell pulse-chase experiments was similar to the purified products in the cortex fraction and in dibucaine-released mucus. Two-dimensional peptide mapping of the 125I-labeled tryptic peptides of three pairs of products in the cortex and mucus further suggested a strong homology. The cleavage of mucocyst precursors was blocked by agents which deplete ATP levels and by N-tosyl-L-phenylalanyl chloromethyl ketone. Preliminary structural relationships were established between some of the precursors and products by one-dimensional peptide mapping. Models for the biogenesis of mucocysts are discussed, and it is proposed that the additional sequence information present in the precursors may be required for the intracellular transport of these proteins or their insertion and assembly within the mucocyst.
- Published
- 1981
27. [Pirprofen hepatitis with a favorable development. Apropos of 5 cases].
- Author
-
Castot A, Netter P, Arnaudo JP, Andrieu J, Vicari F, Ponge B, Wilhelm JM, Danan G, Trechot P, and Frelon JH
- Subjects
- Adult, Aged, Female, Follow-Up Studies, Humans, Male, Middle Aged, Transaminases blood, Anti-Inflammatory Agents, Non-Steroidal adverse effects, Chemical and Drug Induced Liver Injury etiology, Phenylpropionates adverse effects
- Published
- 1984
28. A cortical scaffold in the ciliate Tetrahymena.
- Author
-
Collins T, Baker RL, Wilhelm JM, and Olmsted JB
- Subjects
- Animals, Cell Fractionation, Cilia ultrastructure, Microtubules ultrastructure, Polyethylene Glycols, Tetrahymena ultrastructure
- Published
- 1980
- Full Text
- View/download PDF
29. Fidelity of the eukaryotic codon-anticodon interaction: interference by aminoglycoside antibiotics.
- Author
-
Eustice DC and Wilhelm JM
- Subjects
- Animals, Anticodon, Binding Sites, Codon, Hydrogen-Ion Concentration, Peptide Elongation Factors metabolism, Structure-Activity Relationship, Tetracycline pharmacology, Tetrahymena, Transfer RNA Aminoacylation, Anti-Bacterial Agents pharmacology, Protein Biosynthesis drug effects, Ribosomes metabolism
- Abstract
A homologous in vitro method was developed from Tetrahymena for ribosomal A-site binding of aminoacyl-tRNA to poly(uridylic acid)-programmed ribosomes with very low error frequency. The reaction mixture pH was the crucial factor in the stable A-site association of aminoacyl-tRNA with high fidelity. At a pH greater than 7.1, endogenous activity translocated A-site-bound aminoacyl-tRNA to the P site. If translocation was allowed to occur, a near-cognate amino-acyl-tRNA, Leu-tRNA, could stably bind to the ribosome by translocation to the ribosomal P site. Near-cognate aminoacyl-tRNA did not stably bind to either site when translocation was blocked. Misreading antibiotics stimulated the stable association of near-cognate aminoacyl-tRNA to the ribosomal A site, thereby increasing the error frequency by several orders of magnitude. Ribosome binding of total aminoacyl-tRNA near equilibrium was not inhibited by misreading antibiotics; however, initial rate kinetics of the binding reaction were dramatically altered such that a 6-fold rate increase was observed with paromomycin or hygromycin B. The rate increase was evident with both cognate and near-cognate aminoacyl-tRNAs. Several antibiotics were tested for misreading potency by the ribosome binding method. We found gentamicin G418 greater than paromomycin greater than neomycin greater than hygromycin B greater than streptomycin in the potentiation of misreading. Tetracycline group antibiotics effectively inhibited A-site aminoacyl-tRNA binding without promoting misreading.
- Published
- 1984
- Full Text
- View/download PDF
30. Phenotypic suppression of nonsense mutants in yeast by aminoglycoside antibiotics.
- Author
-
Palmer E, Wilhelm JM, and Sherman F
- Subjects
- Anti-Bacterial Agents pharmacology, Cell-Free System, Codon, Phenotype, Ribosomes drug effects, Structure-Activity Relationship, Aminoglycosides pharmacology, Protein Biosynthesis drug effects, Saccharomyces cerevisiae genetics
- Published
- 1979
- Full Text
- View/download PDF
31. Aminoglycoside antibiotics and eukaryotic protein synthesis: structure--function relationships in the stimulation of misreading with a wheat embryo system.
- Author
-
Wilhelm JM, Pettitt SE, and Jessop JJ
- Subjects
- Amino Acids metabolism, Leucine metabolism, Paromomycin pharmacology, Poly U metabolism, Polyamines pharmacology, Polyribonucleotides metabolism, Structure-Activity Relationship, Triticum genetics, Aminoglycosides pharmacology, Anti-Bacterial Agents pharmacology, Plants genetics, Protein Biosynthesis drug effects
- Published
- 1978
- Full Text
- View/download PDF
32. Altered 40 S ribosomal subunits in omnipotent suppressors of yeast.
- Author
-
Eustice DC, Wakem LP, Wilhelm JM, and Sherman F
- Subjects
- Centrifugation, Density Gradient, Electrophoresis, Genetic Code drug effects, Macromolecular Substances, Paromomycin pharmacology, Protein Biosynthesis, Ribosomal Proteins genetics, Saccharomyces cerevisiae genetics, Suppression, Genetic drug effects
- Abstract
The five suppressors SUP35, SUP43, SUP44, SUP45 and SUP46, each mapping at a different chromosomal locus in the yeast Saccharomyces cerevisiae, suppress a wide range of mutations, including representatives of all three types of nonsense mutations, UAA, UAG and UGA. We have demonstrated that ribosomes from the four suppressors SUP35, SUP44, SUP45 and SUP46 translate polyuridylate templates in vitro with higher errors than ribosomes from the normal stain, and that this misreading is substantially enhanced by the antibiotic paromomycin. Furthermore, ribosomal subunit mixing experiments established that the 40 S ribosomal subunit, and this subunit only, is responsible for the higher levels of misreading. Thus, the gene products of SUP35, SUP44, SUP45 and SUP46 are components of the 40 S subunit or are enzymes that modify the subunit. In addition, a protein from the 40 S subunit of the SUP35 suppressor has an altered electrophoretic mobility; this protein is distinct from the altered protein previously uncovered in the 40 S subunit of the SUP46 suppressor. In contrast to the ribosomes from the four suppressors SUP35, SUP44, SUP45 and SUP46, the ribosomes from the SUP43 suppressor do not significantly misread polyuridylate templates in vitro, suggesting that this locus may not encode a ribosomal component or that the misreading is highly specific.
- Published
- 1986
- Full Text
- View/download PDF
33. Misreading of the ribosomal suppressor SUP46 due to an altered 40 S subunit in yeast.
- Author
-
Masurekar M, Palmer E, Ono BI, Wilhelm JM, and Sherman F
- Subjects
- Leucine metabolism, Mutation drug effects, Paromomycin pharmacology, Phenylalanine metabolism, Protein Biosynthesis drug effects, Ribosomes metabolism, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae genetics, Suppression, Genetic
- Published
- 1981
- Full Text
- View/download PDF
34. Differential translation in normal and adenovirus type 5-infected human cells and cell-free systems.
- Author
-
Cherney CS and Wilhelm JM
- Subjects
- Cell Line, Cell-Free System, Humans, Hypertonic Solutions, Peptide Chain Elongation, Translational drug effects, Peptide Chain Initiation, Translational drug effects, Adenoviridae growth & development, Potassium Chloride pharmacology, Protein Biosynthesis drug effects, Sodium Chloride pharmacology, Viral Proteins biosynthesis
- Abstract
When uninfected or adenovirus 5-infected KB cells are exposed to hypertonic medium, the incorporation of radioactive amino acids into protein decreases in both, but more severely in the uninfected cells. Although the effect of hypertonic medium on the synthesis of specific polypeptides varies, the translation of viral polypeptides as a class is less inhibited. The same patterns of proteins are synthesized regardless of the solute used in the hypertonic medium. The mechanism by which hypertonic conditions exert their effect on whole cells was investigated in K cell-free systems. It was possible to simulate the differential patterns of protein synthesis obtained in whole cells in hypertonic medium by increasing ion concentrations in cell-free extracts which are capable of initiating polypeptide chains on exogenous templates. However, in cell lysates which only elongate proteins, the same patterns were not obtained. Certain host and viral polypeptides displayed striking responses to increased ionic conditions in whole cells and cell-free systems. The synthesis of a host 44K protein, actin, appeared to be most sensitive; lower-molecular-weight proteins were fairly resistant. Among the viral proteins, the synthesis of 100K was inhibited, but most notable was the marked resistance of the synthesis of polypeptide IX. Possible mechanisms for differential synthesis and their significance are considered.
- Published
- 1979
- Full Text
- View/download PDF
35. Mistranslation in a eucaryotic organism.
- Author
-
Palmer E and Wilhelm JM
- Subjects
- Animals, Butirosin Sulfate pharmacology, Cell-Free System, Gentamicins pharmacology, Kanamycin pharmacology, Neomycin pharmacology, Paromomycin analogs & derivatives, Paromomycin pharmacology, Streptomycin pharmacology, Tetrahymena metabolism, Aminoglycosides pharmacology, Anti-Bacterial Agents pharmacology, Protein Biosynthesis drug effects, Tetrahymena drug effects
- Abstract
Previous work from our laboratory has demonstrated that a subclass of the aminoglycoside antibiotics, those containing the drug fragment paromamine, stimulates mistranslation in cell-free protein-synthesizing systems derived from eucaryotic cells. We report here experiments which show that the ciliate Tetrahymena thermophila (formerly T. pyriformis, syngen 1) is sensitive to the paromamine-containing aminoglycoside antibiotics. The drugs are active with respect to growth inhibition, inhibition of protein synthesis in the whole organism, inhibition of protein synthesis in vitro and the stimulation of mistranslation in cell-free protein-synthesizing systems. Because of their misreading properties, these drugs may be useful in isolating and propagating strains carrying mutations which can be translationally suppressed (that is, missense and nonsense mutations).
- Published
- 1978
- Full Text
- View/download PDF
36. Variation of phenotypic suppression due to the psi+ and psi- extrachromosomal determinants in yeast.
- Author
-
Palmer E, Wilhelm JM, and Sherman F
- Subjects
- Codon, Extrachromosomal Inheritance, Mutation drug effects, Paromomycin pharmacology, Protein Biosynthesis drug effects, Phenotype, Saccharomyces cerevisiae genetics
- Published
- 1979
- Full Text
- View/download PDF
37. Mechanisms of action of aminoglycoside antibiotics in eucaryotic protein synthesis.
- Author
-
Eustice DC and Wilhelm JM
- Subjects
- Drug Resistance, Microbial, Eukaryotic Cells metabolism, Lysine metabolism, Peptide Elongation Factors metabolism, Peptides metabolism, Polyribosomes metabolism, RNA, Messenger metabolism, Aminoglycosides pharmacology, Anti-Bacterial Agents pharmacology, Cells drug effects, Eukaryotic Cells drug effects, Protein Biosynthesis, Tetrahymena drug effects
- Abstract
Tetrahymena thermophila is a eucaryotic organism that is highly susceptible to growth inhibition by aminoglycoside antibiotics. Concentrations of paromomycin, gentamicin G418, and hygromycin B at 22, 10, and 17 microM, respectively, inhibited growth by 50%. A combination of in vitro and in vivo methods was used to determine the mechanisms of action of these aminoglycoside antibiotics on protein synthesis in T. thermophila. Analysis of polysome profiles from paromomycin- and gentamicin G418-treated cells showed clear, progressive depletions of polysomes concomitant with an inhibition of in vivo [14C] lysine incorporation. In vitro, paromomycin and gentamicin G418, which are disubstituted 2-deoxystreptamine-containing molecules, were not very effective inhibitors of either the translocation of peptidyl-tRNA or the elongation of nascent polypeptide chains on polysomes. In contrast, we found that the translocation of phe-tRNA on polyuridylate programmed ribosomes was susceptible to inhibition by paromomycin. We conclude that the primary inhibitory action of paromomycin and gentamicin G418 was at (i) an early stage of elongation after initiation, (ii) the initiation stage of translation, or (iii) a stage of translation before initiation. Hygromycin B, which is a monosubstituted 2-deoxystreptamine-containing aminoglycoside, potently inhibited the elongation of nascent chains during the translation of polysomes. In addition, the in vitro translation of polysomes from two hygromycin B-resistant mutants was resistant to the inhibition of elongation caused by hygromycin B.
- Published
- 1984
- Full Text
- View/download PDF
38. Aminoglycoside antibiotics and eukaryotic protein synthesis: stimulation of errors in the translation of natural messengers in extracts of cultured human cells.
- Author
-
Wilhelm JM, Jessop JJ, and Pettitt SE
- Subjects
- Adenoviruses, Human genetics, Cell Line, Cell-Free System, Humans, Leucine metabolism, Paromomycin pharmacology, Poly U metabolism, Viral Proteins biosynthesis, Aminoglycosides pharmacology, Anti-Bacterial Agents pharmacology, Protein Biosynthesis drug effects, RNA, Messenger metabolism
- Published
- 1978
- Full Text
- View/download PDF
39. In vitro synthesis of T4 proteins: control of transcription of gene 57.
- Author
-
Wilhelm JM and Haselkorn R
- Subjects
- Acrylates, Amino Acids, Carbon Isotopes, Cell-Free System, Chloramphenicol, Coliphages metabolism
- Published
- 1971
- Full Text
- View/download PDF
40. Interaction of antibiotics with ribosomes: structure-function relationships and a possible common mechanism for the antibacterial action of the macrolides and lincomycin.
- Author
-
Wilhelm JM, Oleinick NL, and Corcoran JW
- Subjects
- Bacillus subtilis drug effects, Erythromycin pharmacology, Lincomycin pharmacology, Anti-Bacterial Agents pharmacology, Binding Sites drug effects, Ribosomes drug effects
- Published
- 1967
41. In vitro synthesis of T4 proteins: lysozyme and the products of genes 22 and 57.
- Author
-
Wilhelm JM and Haselkorn R
- Subjects
- Autoradiography, Carbon Isotopes, Electrophoresis, Escherichia coli, Genes, Lysine metabolism, Molecular Biology, Mutation, RNA, Messenger pharmacology, RNA, Viral pharmacology, Ribosomes metabolism, Stimulation, Chemical, Time Factors, Virus Cultivation, Coliphages, Muramidase biosynthesis, Viral Proteins biosynthesis
- Published
- 1969
- Full Text
- View/download PDF
42. Specific inhibition of bacteriophage SPO1 DNA-directed protein synthesis by the SPO1 transcription factor, TF 1.
- Author
-
Wilhelm JM, Johnson G, Haselkorn R, and Geiduschek EP
- Subjects
- Aminohydrolases biosynthesis, Carbon Isotopes, Coliphages enzymology, Cytosine Nucleotides, Escherichia coli drug effects, Escherichia coli enzymology, Escherichia coli metabolism, Leucine metabolism, Lysine metabolism, Muramidase biosynthesis, RNA Nucleotidyltransferases antagonists & inhibitors, RNA, Viral pharmacology, Ribosomes drug effects, Ribosomes metabolism, Templates, Genetic, Tritium, Uracil Nucleotides, Bacillus subtilis, Bacteriophages enzymology, DNA, Viral pharmacology, Protein Biosynthesis, Viral Proteins pharmacology
- Published
- 1972
- Full Text
- View/download PDF
43. Antibiotic glycosides. VI. Definition of the 50s ribosomal subunit of Bacillus subtilis 168 as a major determinant of sensitivity to erythromycin A.
- Author
-
Wilhelm JM and Corcoran JW
- Subjects
- Adenosine Triphosphate pharmacology, Carbon Isotopes, Kinetics, Lysine metabolism, Mutation, Phenylalanine metabolism, Polynucleotides chemical synthesis, Proline metabolism, Pyruvate Kinase metabolism, RNA, Transfer pharmacology, Bacillus subtilis drug effects, Erythromycin pharmacology, Glycosides, Peptide Biosynthesis, Ribosomes metabolism
- Published
- 1967
- Full Text
- View/download PDF
44. Synthesis in vitro of type 5 adenovirus capsid proteins.
- Author
-
Wilhelm JM and Ginsberg HS
- Subjects
- Adenoviridae immunology, Amino Acids metabolism, Animals, Carbon Isotopes, Carcinoma, Cell Fractionation, Cell Line microbiology, Cell-Free System, Centrifugation, Density Gradient, Chemical Precipitation, Cytoplasm metabolism, Dithiothreitol, Humans, Immune Sera, Kinetics, Mouth Neoplasms, Peptide Biosynthesis, Rabbits immunology, Ribosomes metabolism, Serotyping, Valine metabolism, Viral Proteins isolation & purification, Adenoviridae metabolism, Viral Proteins biosynthesis
- Abstract
Reaction mixtures containing cytoplasmic extracts and ribosomal fractions prepared from KB cells infected with type 5 adenovirus were able to carry out incorporation of amino acids into protein. The in vitro product included proteins which reacted specifically with antisera to adenovirus capsid proteins; in control experiments with extracts from uninfected cells, no reactions with the antisera were found. The viral proteins were synthesized in vitro on small polyribosomes, were released from them, and significant numbers of the free polypeptides were assembled in vitro into multimeric adenovirus capsid structures.
- Published
- 1972
- Full Text
- View/download PDF
45. The chain growth rate of T4 lysozyme in vitro.
- Author
-
Wilhelm JM and Haselkorn R
- Subjects
- Carbon Isotopes, Coliphages cytology, Cyclohexanecarboxylic Acids pharmacology, Lysine metabolism, Peptides metabolism, Polynucleotides pharmacology, RNA, Transfer metabolism, Viral Proteins biosynthesis, Coliphages enzymology, Muramidase biosynthesis, RNA, Messenger metabolism, Ribosomes metabolism
- Abstract
The average interval between attachment of a 30S ribosomal subunit to bacteriophage T4 lysozyme mRNA and the completion of synthesis of lysozyme protein has been measured in vitro. The measured completion times yield rates of polypeptide chain propagation of 1.6 and 3.0 amino acids per second at 25 degrees C and 31 degrees C, respectively.
- Published
- 1970
- Full Text
- View/download PDF
46. In vitro synthesis of T4 proteins: the products of genes 9, 18, 19, 23, 24, and 38.
- Author
-
Wilhelm JM and Haselkorn R
- Subjects
- Acrylates, Amino Acids, Autoradiography, Carbon Isotopes, Cell-Free System, Coliphages metabolism
- Published
- 1971
- Full Text
- View/download PDF
47. Nonidentity of the site of action of erythromycin A and chloramphenicol on Bacillus subtilis ribosomes.
- Author
-
Oleinick NL, Wilhelm JM, and Corcoran JW
- Subjects
- Bacillus subtilis growth & development, Carbon Isotopes, Chloramphenicol pharmacology, Drug Resistance, Microbial, Lysine metabolism, Peptide Biosynthesis, Ribosomes metabolism, Bacillus subtilis drug effects, Erythromycin pharmacology, Receptors, Drug, Ribosomes drug effects
- Published
- 1968
- Full Text
- View/download PDF
48. The inhibition of bacterial RNA synthesis by the rifamycin antibiotics.
- Author
-
Wilhelm JM, Oleinick NL, and Corcoran JW
- Subjects
- Bacillus subtilis drug effects, Cell-Free System, Depression, Chemical, Drug Resistance, Microbial, Escherichia coli enzymology, Genetics, Microbial, Lysine, Mutation, Phenylalanine, RNA Nucleotidyltransferases, Anti-Bacterial Agents pharmacology, Bacillus subtilis metabolism, Peptide Biosynthesis, RNA, Bacterial biosynthesis, Rifampin pharmacology
- Published
- 1968
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.