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Simple Summary: This study presents an integrated conservation framework for the globally threatened fishing cat, synthesizing both ex situ management and in situ strategies. The strategy aligns with the One Plan Approach, which views captive populations as part of a broader metapopulation network, and the Opportunities to Thrive framework, which emphasizes animal welfare in captivity. This study analyzed the genetic diversity of a recently initiated fishing cat captive breeding population by the West Bengal Zoo Authority, India, and conducted a population viability analysis. The results suggest that the current genetic diversity (56%) and population carrying capacity (30 individuals) can be sustained for over 100 years through a strategy of supplementation and harvesting. Introducing two adult males and females annually will allow for sustainable harvesting to supplement wild populations. Additionally, this study identified 21 potential reintroduction zones in the Sundarbans and Terai regions of West Bengal, using environmental criteria that favor fishing cat occurrence. This work offers a comprehensive blueprint for applying the One Plan Approach to ensure the long-term survival of the fishing cat. The One Plan Approach advocates for a hybrid species management framework, wherein captive-bred populations are considered metapopulations nested within a broader network of zoos and wild populations Additionally, the Opportunities to Thrive framework aims to enhance animal welfare by addressing the physiological, psychological, and emotional needs of captive individuals, thereby improving conservation outcomes. Here, we present an integrated framework for the conservation of a globally threatened wetland wild cat species, the fishing cat, by synthesizing optimal ex situ management practices and in situ conservation strategies. Further, we examined the genetic constitution of the founder population in a fishing cat captive breeding program that was recently initiated by the West Bengal Zoo Authority, India and conducted a population viability analysis to suggest how best to maintain the genetic diversity of the population. We found that the present genetic diversity of 56% and maximum carrying capacity of the captive population (30 individuals) can be maintained for more than 100 years with a combination of supplementation and harvesting. Keeping stochastic events in mind, the introduction of two adult males and females to the existing population each year will seamlessly allow the harvesting of two adult males and two adult females every alternate year to supplement wild populations. Further, we adopted the proposed integrated framework to delineate recommendations for the supplementation of wild populations in West Bengal. We used environmental criteria known to influence fishing cat occurrence to identify 21 potential reintroduction zones in the Sundarbans landscape and Terai region in northern West Bengal with habitable areas for the fishing cat that are larger than the maximum known species' home range. Our study is timely and insightful because it provides a holistic blueprint for implementing the One Plan Approach in safeguarding a threatened species. [ABSTRACT FROM AUTHOR]

Simple Summary: In Thailand, the flat-headed cat, fishing cat, and Asiatic golden cat are among nine wild Felidae species requiring urgent conservation efforts. This study assessed routine sperm characteristics and detected protein biomarkers related to fertilization in these species. IZUMO1, involved in gamete interaction, showed the highest levels in the flat-headed cat. CRISP2 and CRISP3, crucial for sperm–egg fusion, had the highest levels in the fishing cat. Cleaved caspase-3, linked to DNA fragmentation, was found in all species and associated with decreased semen quality. These findings suggest that analyzing these biomarkers, alongside routine sperm characteristics, could enhance breeding management for wild Felidae, especially the flat-headed and fishing cats. Effective wild cat conservation programs with assisted reproductive technologies are being developed in different parts of the world. The flat-headed cat, fishing cat, and Asiatic golden cat are three species among nine wild Felidae in Thailand that are in need of urgent conservation efforts. Here, we assessed routine sperm characteristics and we report the detection of protein biomarkers related to the fertilization process, IZUMO1 and the CRISP family, and apoptotic markers, active or cleaved caspase-3, in semen samples collected from these wild cats. IZUMO1 was located in the equatorial segment of the sperm head, which is the region involved in gamete interaction. The highest levels of IZUMO1 were found in both the sperm pellet and the seminal plasma of the flat-headed cat, as determined by immunoblotting. CRISP2, a sperm–egg fusion assisting protein, and CRISP3 were found in both the sperm pellet and the seminal plasma, and the highest levels were observed in the fishing cat. Positive correlations between certain semen parameters and IZUMO1, CRISP2, and CRISP3 expression were also demonstrated. Cleaved caspase-3 was found in all sperm samples in all three species and was associated with an increase in DNA fragmentation and a decrease in certain semen characteristics such as motility, viability, and intact acrosomes. Our results suggest that the analysis of IZUMO1, the CRISP family, and cleaved caspase-3, along with the routine sperm characteristics, may allow for better success in breeding management in wild Felidae, particularly in the flat-headed cat and the fishing cat. [ABSTRACT FROM AUTHOR]

Introduction: Human-wildlife interactions are on the rise due to anthropogenic pressures. Understanding these interactions is crucial for preventing conflict and promoting coexistence, ultimately benefitting humans and wildlife. Objective: In this study, we analysed the interactions between humans and jaguars Panthera onca in Tortuguero National Park, Costa Rica. Methods: We examined data concerning jaguar sightings within Tortuguero National Park, Costa Rica compiled from 2000 to 2022. Results: A total of 381 jaguar sightings were recorded, of which 75% occurred in the coastal habitat; 81% occurred during the leatherback sea turtle Dermochelys coriacea (March-May) and the green sea turtle Chelonia mydas (June-October) nesting seasons. From photographic records, 26 individual jaguars were identified (14 females, 12 males). Furthermore, 9% of the sightings were reported within the boundaries of Tortuguero village, where at least 48 domestic dogs Canis lupus familiaris were attacked by jaguars. Conclusions: Despite the jaguar's cryptic nature, the data show an increase in the frequency and duration of jaguar sightings over the years. This trend is likely due to an increase in the local jaguar population and possibly an increase in jaguars' tolerance towards humans, possibly triggered by people's inappropriate behaviour (e.g. approaching jaguars while feeding, mating or with their cubs) during the sightings. Our study also highlights the potential for conflict due to jaguar predation of domestic dogs, which is increasing within the study area. Therefore, there is a need for management action to promote coexistence between humans and the local jaguar population in Tortuguero National Park, Costa Rica.

The One Plan Approach advocates for a hybrid species management framework, wherein captive-bred populations are considered metapopulations nested within a broader network of zoos and wild populations Additionally, the Opportunities to Thrive framework aims to enhance animal welfare by addressing the physiological, psychological, and emotional needs of captive individuals, thereby improving conservation outcomes. Here, we present an integrated framework for the conservation of a globally threatened wetland wild cat species, the fishing cat, by synthesizing optimal ex situ management practices and in situ conservation strategies. Further, we examined the genetic constitution of the founder population in a fishing cat captive breeding program that was recently initiated by the West Bengal Zoo Authority, India and conducted a population viability analysis to suggest how best to maintain the genetic diversity of the population. We found that the present genetic diversity of 56% and maximum carrying capacity of the captive population (30 individuals) can be maintained for more than 100 years with a combination of supplementation and harvesting. Keeping stochastic events in mind, the introduction of two adult males and females to the existing population each year will seamlessly allow the harvesting of two adult males and two adult females every alternate year to supplement wild populations. Further, we adopted the proposed integrated framework to delineate recommendations for the supplementation of wild populations in West Bengal. We used environmental criteria known to influence fishing cat occurrence to identify 21 potential reintroduction zones in the Sundarbans landscape and Terai region in northern West Bengal with habitable areas for the fishing cat that are larger than the maximum known species’ home range. Our study is timely and insightful because it provides a holistic blueprint for implementing the One Plan Approach in safeguarding a threatened species.

The wild cat (Felis silvestris), spread in Romania from the Danube Delta to the mountain range is present in the Banat area, on the hunting ground that can be contaminated with different stage developmental forms of parasites, some of them having real zoonotic potential. The wild cat is an animal protected by the Romanian law of protection animals.

While the use of trees has been widely documented for most of the big cats, this information is scant for the jaguar (Panthera onca). In this study, we investigated the use of trees by jaguars, evaluating different underlying reasons as well as the frequency of tree use based on sex, age, and reproductive status. Data were obtained from 2013 to 2020 through a combination of direct observations during fieldwork and camera trapping focused on this behavior in the Brazilian Pantanal. We documented 252 climbing events (176 direct observations, 76 camera captures). Using only camera trapping data to avoid observers' influence on jaguar behavior, we fitted generalized linear mixed models (GLMM) with jaguar identity as a random factor to test the effects of age, sex, and presence of conspecifics in the odds of animals climbing trees. We also used a generalized linear model (GLM) to test the effect of adult body weight on jaguar tree climbing probability. Age and presence of conspecifics were the most important drivers of jaguar climbing behavior in the study area, with cubs being more likely to climb trees than adults of either sex (odds ratio = 7.91, p < 0.001), and the presence of conspecifics, irrespective of age and sex, accentuated that behavior (odds ratio = 3.26, p < 0.005). Adult females (especially the lighter ones) and cubs were commonly recorded through direct observation on trees, a similar trend to our GLMM that showed a marginally negative effect of body weight on jaguar tree climbing probability. Only a few adult male jaguars ascended trees, and in all cases, these males were following females in heat. We suggest that trees are vertical extensions inside jaguar home ranges and may be safe refuges for resting and for protecting offspring against potential threats, including the harassment of large adult males. [ABSTRACT FROM AUTHOR]

Leopard cat (Prionailurus bengalensis euptilurus) is a small wild cat assessed as an endangered wildlife in Korea. There have been very few reports of their diseases. Herein, we describe fibrinous pleuritis caused by Streptococcus canis infection with excessive pleural effusion, hydropericardium, mild ascites, and liver fibrosis in a leopard cat. S. canis is a commensal microflora in domestic cats and often affects the upper respiratory tract inducing chronic and severe respiratory diseases. However, there is no literature regarding the S. canis in leopard cats. Therefore, we first report fibrinous pleuritis associated with an S. canis infection in a leopard cat. [ABSTRACT FROM AUTHOR]

Effective wild cat conservation programs with assisted reproductive technologies are being developed in different parts of the world. The flat-headed cat, fishing cat, and Asiatic golden cat are three species among nine wild Felidae in Thailand that are in need of urgent conservation efforts. Here, we assessed routine sperm characteristics and we report the detection of protein biomarkers related to the fertilization process, IZUMO1 and the CRISP family, and apoptotic markers, active or cleaved caspase-3, in semen samples collected from these wild cats. IZUMO1 was located in the equatorial segment of the sperm head, which is the region involved in gamete interaction. The highest levels of IZUMO1 were found in both the sperm pellet and the seminal plasma of the flat-headed cat, as determined by immunoblotting. CRISP2, a sperm–egg fusion assisting protein, and CRISP3 were found in both the sperm pellet and the seminal plasma, and the highest levels were observed in the fishing cat. Positive correlations between certain semen parameters and IZUMO1, CRISP2, and CRISP3 expression were also demonstrated. Cleaved caspase-3 was found in all sperm samples in all three species and was associated with an increase in DNA fragmentation and a decrease in certain semen characteristics such as motility, viability, and intact acrosomes. Our results suggest that the analysis of IZUMO1, the CRISP family, and cleaved caspase-3, along with the routine sperm characteristics, may allow for better success in breeding management in wild Felidae, particularly in the flat-headed cat and the fishing cat.

The Tsushima leopard cat (Prionailurus bengalensis euptilurus) is a subspecies of the mainland leopard cat that lives on the small island of Tsushima, Japan. Captive breeding has been attempted in zoos in Japan because only approximately 100 animals remain in the wild and the Tsushima leopard cat is an endangered species. There are very few reports on diseases, including tumours, of this species. We analysed the deaths of 58 Tsushima leopard cats and confirmed that nine had neoplastic disease. The average age at death of the animals with neoplasia was 14 years and tumours were the primary cause of death in all animals. Eight of the nine cases involved primary tumours of the pancreas, liver, gallbladder, tongue and salivary glands, suggesting that Tsushima leopard cats may have a predilection for digestive system tumours. This is the first report of neoplastic disease in the Tsushima leopard cat. [ABSTRACT FROM AUTHOR]

Keywords: Felis wiedii; Leopardus macrourus; Leopardus wiedii; margay; Reise nach Brasilien; Heinrich Rudolf Schinz; wild cat EN Felis wiedii Leopardus macrourus Leopardus wiedii margay Reise nach Brasilien Heinrich Rudolf Schinz wild cat 412 415 4 11/23/22 20221001 NES 221001 The margay, I Leopardus wiedii i [16], is a Neotropical small spotted cat, whose nomenclatural history has long been confused ([20]; [14]; [1]). First description of Felis macroura by Alexander Philipp Maximilian, Prince zu Wied-Neuwied, Reise nach Brasilien in den Jahren 1815 bis 1817 (1820, part II: 204). Leopardus macrourus, Reise nach Brasilien, Heinrich Rudolf Schinz, Felis wiedii, Leopardus wiedii, margay, wild cat. [Extracted from the article]

This note presents the first record of Ocelot (Leopardus pardalis) in a rural area in the municipality of Santa Rosa de Cabal. This finding was obtained by a camera trap. In addition, there were two records by habitants of the sector. These records are of great importance since they are the first in the department of Risaralda outside of protected areas, so arises the idea of implementing monitoring and conservation plans.

Abstract Background Benign juvenile idiopathic epilepsy has been described in humans but rarely in animals. The objectives of the study were to describe the clinical signs, clinical data, imaging findings, genetic examinations, treatment, long-term outcome and prognosis in Iberian lynx with juvenile epilepsy. Medical records, video recordings and diagnostic data from 2005 to 2019 were reviewed. Results Twenty lynx cubs with early onset of epileptic seizures (ES) from the conservation program were included. The average age at seizure onset was 75 days. Isolated and cluster ES were recorded. Focal ES, focal ES evolving into generalized ES with a stereotypical pattern and generalized ES were observed. All the cubs were normal between episodes, had a normal neurological examination and unremarkable investigations. Phenobarbital was used as a first line antiepileptic drug (AED). ES halted 10 days (0–34) after starting treatment in eight out of twenty cubs (40%). Treatment was discontinued in this group after a mean of 578 days and no further ES were reported (mean follow-up longer than 5 years). Eleven animals (55%) continued on AED treatment for a mean of 1306 days (70–3466). An adult-onset was observed for one lynx (5%). Polytherapy was necessary in seven lynxes (35%). The inheritance pattern observed was compatible with an autosomal recessive condition. Based on this assumption, mating between two identified carriers has been avoided since 2012, which may have contributed to the subsequent decrease in prevalence, with no further cases detected in 2018 and 2019. Conclusions Lynx pardinus may have an early onset self-limiting ES syndrome characteristic of benign juvenile idiopathic epilepsy. Information obtained from this study strongly suggests a genetic basis for the here presented epilepsy.

The African golden cat, Caracal aurata, ranges from west through central to east Africa and is categorized as Vulnerable on the IUCN Red List of Threatened Species. Coenraad J. Temminck (1778–1858) described Felis aurata (African golden cat) and other new felid species in the fourth of seven monographs collected in the first volume of his Monographies de mammalogie, which was dated 1827 on the title-page. For nearly two centuries, Temminck's description of Felis aurata has therefore been cited as published in 1827. However, this is in error because the different monographs were published as they were completed and later compiled into volumes. The actual date of issue for the fourth monograph was 1825, so that the correct current citation for the African golden cat should be Caracal aurata (Temminck, 1825). This revised citation date is also applicable to the Iberian lynx, Lynx pardinus (Temminck, 1825), which is categorized as Endangered on the IUCN Red List. [ABSTRACT FROM AUTHOR]

Aelurostrogylus abstrusus is a helminthic pathogen that infects the respiratory tract of felids after the ingestion of intermediate or paratenic hosts. The present paper assesses A. abstrusus infection in domestic and wild cats in south of Brazil. Fecal samples from 97 domestic cats and from seven wild cats (Puma concolor, Puma yagouaroundi and Leopardus tigrinus) were tested using sedimentation and flotation techniques. The prevalence of first-stage larvae was 2.1% (2/97) in domestic cats, and Puma yagouaroundi was the only wild cat that turned out to be positive for this lungworm. This is the first report of A. abstrusus in Puma yagouaroundi in Brazil. The identification of this parasite raises a red flag about the presence of infected intermediate and/or paratenic hosts and about the importance of this parasitic disease even in the absence of respiratory clinical signs. The analysis of fecal samples is then suggested for the detection of A. abstrusus. [ABSTRACT FROM AUTHOR]

Abstract Background Felids (domestic and wild cats) are important in the epidemiology of the parasite Toxoplasma gondii because they are the only hosts that can excrete the environmentally resistant oocysts. We conducted a systematic review and meta-analysis to estimate the global prevalence of T. gondii in species of the family Felidae. Methods We searched seven databases (PubMed, Embase, Google Scholar, ScienceDirect, Scopus, Proquest and Web of Science) for studies reporting seroprevalence of T. gondii in felids from 1967 to 31 December 2017. A total of 217 published papers, containing 223 datasets were eligible for inclusion in the meta-analysis, comprised 59,517 domestic and 2733 wild cats from 1967 to 2017. Results The pooled global T. gondii seroprevalence was estimated to be 35% (95% CI: 32–38%) and 59% (95% CI: 56–63%) in domestic cats and wild felids, respectively, using random effects model. The seroprevalence was higher in Australia and Africa where the T. gondii seropositivity in domestic cats was 52% (95% CI: 15–89%) and 51% (95% CI: 20–81%), respectively. The lowest seroprevalence was estimated in Asia 27% (95% CI: 24–30%). The seroprevalence values for T. gondii in wild felids were 74% (95% CI: 62–83%) in Africa, 67% (95% CI: 23–111%) in Asia, 67% (95% CI: 58–75%) in Europe and 66% (95% CI: 41–91%) in South America. Conclusions Our study provides the global prevalence of T. gondii in species of the family Felidae and is a source of information to aid public health workers in developing prevention plans.

During 2016–2019, new data on the wildcat’s distribution in Khmelnytskyi, Vinnytsia, and Ternopil regions were obtained. The data concern 4 findings of the wildcat whereof 3 findings are dead specimens (a dead female in a poacher’s automatic trap, 2 specimens (male and female) hunted by a poacher, one specimen killed during the red fox population control measures in Podilski Tovtry National Nature Park, and one specimens recorded by a camera trap in Medobory Nature Reserve. The article provides detailed data on new findings of the species in Podillia and describes the environmental factors influencing its population, as well as provides recommendations on protection of the wildcat in the region. The frequency of occurrence of the wildcat in Podillia has been growing for several years. Nevertheless, the data remain incidental and occasional thus cannot indicate the growth of the species abundance in the region. Most of the located specimens were found dead on roads, in poacher traps or even killed during population control measures in the territory of protected areas. According to the information provided by Kamianets-Podilskyi organization of the Ukrainian Hunter and fishermen Society, its associate hunting teams annually obtain more than 100 specimens of feral cats, among which supposedly there is a great number of wildcats. Currently, spaying feral cats is the key element for preservation of the wildcat population in general, nevertheless, this aim remains unfulfilled only because this issue is not addressed properly thus a significant number of intact pets maintains contacts with the wildcat outside the human settlements. Ecological educational programs are an essential measure for the wildcat protection, especially in its habitats, though usually such work is aimed at both current and remote prospects. It is expected that a ban for hunting of feral cats in habitats of the wildcat implemented by including the appropriate paragraph into the population control permit issued by the Ecology and Natural Resources Department should enhance the species’ preservation in the near future. Availability of long-distance photographic equipment for locating the wildcat (such as camera traps) and monitoring system implementation at least on sites of protected areas would provide the required data on the abundance and distribution of the wildcat in the region allowing to improve measure for its protection and conservation.

Aelurostrogylus abstrusus is a helminthic pathogen that infects the respiratory tract of felids after the ingestion of intermediate or paratenic hosts. The present paper assesses A. abstrusus infection in domestic and wild cats in south of Brazil. Fecal samples from 97 domestic cats and from seven wild cats (Puma concolor, Puma yagouaroundi and Leopardus tigrinus) were tested using sedimentation and flotation techniques. The prevalence of first-stage larvae was 2.1% (2/97) in domestic cats, and Puma yagouaroundi was the only wild cat that turned out to be positive for this lungworm. This is the first report of A. abstrusus in Puma yagouaroundi in Brazil. The identification of this parasite raises a red flag about the presence of infected intermediate and/or paratenic hosts and about the importance of this parasitic disease even in the absence of respiratory clinical signs. The analysis of fecal samples is then suggested for the detection of A. abstrusus. [ABSTRACT FROM AUTHOR]

Background: Benign juvenile idiopathic epilepsy has been described in humans but rarely in animals. The objectives of the study were to describe the clinical signs, clinical data, imaging findings, genetic examinations, treatment, long-term outcome and prognosis in Iberian lynx with juvenile epilepsy. Medical records, video recordings and diagnostic data from 2005 to 2019 were reviewed. Results: Twenty lynx cubs with early onset of epileptic seizures (ES) from the conservation program were included. The average age at seizure onset was 75 days. Isolated and cluster ES were recorded. Focal ES, focal ES evolving into generalized ES with a stereotypical pattern and generalized ES were observed. All the cubs were normal between episodes, had a normal neurological examination and unremarkable investigations. Phenobarbital was used as a first line antiepileptic drug (AED). ES halted 10 days (0–34) after starting treatment in eight out of twenty cubs (40%). Treatment was discontinued in this group after a mean of 578 days and no further ES were reported (mean follow-up longer than 5 years). Eleven animals (55%) continued on AED treatment for a mean of 1306 days (70–3466). An adult-onset was observed for one lynx (5%). Polytherapy was necessary in seven lynxes (35%). The inheritance pattern observed was compatible with an autosomal recessive condition. Based on this assumption, mating between two identified carriers has been avoided since 2012, which may have contributed to the subsequent decrease in prevalence, with no further cases detected in 2018 and 2019. Conclusions: Lynx pardinus may have an early onset self-limiting ES syndrome characteristic of benign juvenile idiopathic epilepsy. Information obtained from this study strongly suggests a genetic basis for the here presented epilepsy. [ABSTRACT FROM AUTHOR]

Background: Felids (domestic and wild cats) are important in the epidemiology of the parasite Toxoplasma gondii because they are the only hosts that can excrete the environmentally resistant oocysts. We conducted a systematic review and meta-analysis to estimate the global prevalence of T. gondii in species of the family Felidae. Methods: We searched seven databases (PubMed, Embase, Google Scholar, ScienceDirect, Scopus, Proquest and Web of Science) for studies reporting seroprevalence of T. gondii in felids from 1967 to 31 December 2017. A total of 217 published papers, containing 223 datasets were eligible for inclusion in the meta-analysis, comprised 59,517 domestic and 2733 wild cats from 1967 to 2017. Results: The pooled global T. gondii seroprevalence was estimated to be 35% (95% CI: 32–38%) and 59% (95% CI: 56–63%) in domestic cats and wild felids, respectively, using random effects model. The seroprevalence was higher in Australia and Africa where the T. gondii seropositivity in domestic cats was 52% (95% CI: 15–89%) and 51% (95% CI: 20–81%), respectively. The lowest seroprevalence was estimated in Asia 27% (95% CI: 24–30%). The seroprevalence values for T. gondii in wild felids were 74% (95% CI: 62–83%) in Africa, 67% (95% CI: 23–111%) in Asia, 67% (95% CI: 58–75%) in Europe and 66% (95% CI: 41–91%) in South America. Conclusions: Our study provides the global prevalence of T. gondii in species of the family Felidae and is a source of information to aid public health workers in developing prevention plans. [ABSTRACT FROM AUTHOR]

Abstract The jaguar is categorized as "Near Threatened". Conservation strategies, therefore, are needed which include use of reproductive biotechniques. For implementation of biotechnique use, the reproductive characteristics of the species must be understood, which is currently not the case. This study, therefore, aimed to describe the detailed morphology of jaguar sperm, and to evaluate the sperm mitochondrial activity. Five male adults were used. Slides stained with Rose Bengal were used for morphometric and morphological analyses. The length and the width of the sperm head were measured, as well as the length of the middle piece, the tail, and the total length. Scanning and transmission electron microscopy were used for ultrastructural analysis. Mitochondrial function was assessed using the marker 3,3′-diaminobenzidine (DAB). The results are expressed as means ± SEM. The most significant morphological abnormalities observed were head (9 ± 1.7%) and tail defects (12.5 ± 3.3%). The width and length of the head were 3.6 ± 0.03 μm and 4.9 ± 0.02 μm, respectively. The middle piece measured 9.7 ± 0.3 μm, the tail measured 54.5 ± 4.4 μm, and the total length of the sperm was 59.5 ± 0.1 μm. Electron-lucent regions and approximately 54 mitochondrial spirals in the middle piece were identified in the nucleus using electron microscopy. The greatest percentages of cells were classified as DAB I (46.6 ± 4.9%) and DAB II (38 ± 4.4%). The data provide detailed information on the sperm characteristics of jaguars and can support research on germplasm conservation for the species. [ABSTRACT FROM AUTHOR]

This work was developed at Dr. Francisco Maeda - FAFRAM campus in the city of Ituverava, SP, aimed to elaborate a protocol for analysis of crossbreeding occurrence between domestic and wildcat species, and also to scientifically confirm this occurrence which still non-existent in Brazil. Genetic material of two litters of cats supposedly descending from natural crossing between domestic female cats and a male wildcat were analyzed. For such analysis, two markers associated with the Y chromosome were employed, SMCY and SRY, intending to identify the paternal lineage, and an ATP6 mitochondrial marker, to identify the maternal lineage. The results indicated that the used markers were adequate and sufficient to confirm or to exclude the occurrence of hybridization, in both paternal and maternal lineages, and could be used as protocol for such analysis. On the other hand, this work concluded that the animals belonged to the domestic cat species (Felis silvestris catus L.), discarding any possibility of hybridization with wildcats of the Brazilian fauna. [ABSTRACT FROM AUTHOR]

Objective To establish baseline normal scotopic electroretinograpic (ERG) parameters for two wild cat species: fishing cats (FC) and leopard cats (LC). Animal studied Twelve normal, FC and eight LC kept in the Chiang Mai Night Safari Zoo, Thailand. The mean ages of FC and LC were 7.08 and 5.00 years, respectively. Procedure All animals were studied using a standard scotopic protocol of a portable, handheld, multi-species electroretinography (HMsERG). Results There were significant differences in the means of ERG b-wave amplitude of the rod response (Rod, 0.01 cd.s/m2), a- and b-wave amplitudes of standard light intensity of rod and cone response (Std R&C, 3 cd.s/m2) and b-wave amplitude of high light intensity of rod and cone response (Hi-int R&C, 10 cd.s/m2) with LC having higher amplitudes than FC. There was no significant difference in a- and b- wave implicit time except for the b-wave of Hi-int (P=0.03). No significant differences were observed in b/a amplitude ratios. Conclusions Data from this report provides reference values for scotopic ERG measurements in these two wild cat species. It showed that the normal scotopic ERG responses have some differences between the two species which might be due to the skull conformation, eye size or physiology of the retina. [ABSTRACT FROM AUTHOR]

The historical literature suggests that in Australia, the domestic cat (Felis catus) had a European origin [~200 years before present (ybp)], but it is unclear if cats arrived from across the Asian land bridge contemporaneously with the dingo (4000 ybp), or perhaps immigrated ~40 000 ybp in association with Aboriginal settlement from Asia. The origin of cats in Australia is important because the continent has a complex and ancient faunal assemblage that is dominated by endemic rodents and marsupials and lacks the large placental carnivores found on other large continents. Cats are now ubiquitous across the entire Australian continent and have been implicit in the range contraction or extinction of its small to medium sized (<3.5 kg) mammals. We analyzed the population structure of 830 cats using 15 short tandem repeat (STR) genomic markers. Their origin appears to come exclusively from European founders. Feral cats in continental Australia exhibit high genetic diversity in comparison with the low diversity found in populations of feral cats living on islands. The genetic structure is consistent with a rapid westerly expansion from eastern Australia and a limited expansion in coastal Western Australia. Australian cats show modest if any population structure and a close genetic alignment with European feral cats as compared to cats from Asia, the Christmas and Cocos (Keeling) Islands (Indian Ocean), and European wildcats (F. silvestris silvestris). [ABSTRACT FROM AUTHOR]

The article presents a study on the explanation of the origin and distribution of collateral branches from abdominal aorta in ocelot (Leopardus pardalis). It mentions that the analysis was performed on two ocelots including a female and a male. It concludes that the abdominal arterial vasculature of ocelots is similar to that in domestic cats and other mammals, differing in number of the jejunal arteries and origin of the renal arteries.

Abstract: Six free-ranging European beavers (Castor fiber) from Berlin greater metropolitan area and twelve European wildcats (Felis silvestris silvestris) originating from the German Federal State of Saxony-Anhalt were found dead and their carcasses were submitted for necropsy. Brain and lung samples were analysed for the presence of Toxoplasma gondii DNA. Histo-pathologic analysis of one beaver revealed several cyst-like protozoal structures in parts of the brain. Tissue DNA isolated from all animal samples was analysed by a specific T. gondii-PCR. Two beavers and four wildcats tested T. gondii-positive. DNA of the parasites was further analysed by PCR-RFLP typing using nine markers (nSAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico). Only T. gondii type II alleles were detected, except for the Apico locus, where type I alleles were observed in two isolates from beavers and in three from wild cats. The results of this study indicate that type II T. gondii (including type II variant strain) is the most common genotype infecting wildcats and beavers from Germany. [Copyright &y& Elsevier]

The present article constitutes a monograph of educational and informative character in the environment of the geographical and environmental sciences, being dedicated to give to know the situation that experiences the autochthonous fauna of the V Region of Valparaíso, as well as the natural vegetation that is own and representative of this part of "Temperate Mediterranean Chile", as - for example - the palm of coconuts or Khan - kán Oubaea spectabilis), the thorn (Acacia cavenia), the boldo (Peumus boldus), the belloto of the north, the lúcumo, the quillay (Quillaja saponaria), the peumo (Cryptocaria alba), the molle, the litre (Litraea caustica) and the guayacán, among others. Finally, the article presents inherent bibliographical information to the British naturalist Charles Darwin's trip through "Temperate Mediterranean Chile" in the 1830's and where is given to know the route for him followed and the activities developed from the port of Valparaíso and until the Andean sector of the valley of the river Aconcagua, area where it made interesting observations regarding the existent wild life in this part of "Southern America". [ABSTRACT FROM AUTHOR]

The influence of chronic hypertension (HT) on the cerebral and renal arteries was examined pathologically and morphometrically in wild cats without a specific genetic background. Chronic HT for 8–15 months was induced by uninephrectomy and salt-loading, and the blood pressure was monitored for a maximum of 5 months. The grade of systolic blood pressure elevation in each cat during the monitoring period was 21–51 mmHg. Histologically, the cerebral arachnoid and medullary arteries of all hypertensive cats showed a well-preserved medial layer, and neither loss of medial smooth muscle cells, adventitial fibrosis or fibrinoid exudation was detected. This experimental model of chronic HT in wild cats for 8–15 months induced segmental intimal elastofibrosis of the arachnoid and renal arteries, but spared the cerebral medullary artery. The parenchymal changes in the brain were negligible. Morphometrically, the arachnoid artery in control cats had a significantly thinner media than the renal artery, and the medial hypertrophy of the arachnoid artery resulting from HT occurred significantly less frequently than that of the renal artery. These findings suggest that the arachnoid and medullary arteries are relatively well protected from HT, and that this may be characteristic of cerebral arteries in general and ascribed to autoregulation. [ABSTRACT FROM AUTHOR]

Wildcats (Felis silvestris) spread over Europe ranging from Spanish to Eastern woodlands. In Northern Africa, the wildcat belongs to a different subspecies (Felis silvestris libyca) which diffused in Sardinia giving rise to a novel taxon there (Felis silvestris libyca sarda). The morphological features of the Sardinian wildcat taxon are not well-known yet. In our work its morphological and morphometrical features have been studied on 19 stuffed models. Numerous morphometrical parameters were taken, and the distribution of spots and stripes of the coat was studied. A low variability in the morphometrical parameters was detected, confirming that the Sardinian wildcat can be considered a well-defined taxon. Since one of the most severe problems in the safeguard of pure Sardinian wildcats is their crossbreeding with domestic cats, the comprehensive knowledge of the anatomical features of these animals could be useful in making a distinction between these two taxa [ABSTRACT FROM AUTHOR]

Adipose derived mesenchymal stem cells (AMSCs) have been isolated from domestic and wild cats. For wild cats, the isolation of AMSCs has been reported in the black-footed cats ( Felis nigripes ) and guigna ( Leopardus guigna ). Stromal vascular fraction (SVF) isolated from cougar adipose tissue have been used to restore elbow functionality in the cougar ( Puma concolor ) but multipotent characteristics of these cells have not been described. The present study describes for the first time the isolation and characterization of mesenchymal stem cells derived from adipose tissue of cougar. AMSCs and fibroblasts from six months female cougar were isolated and cultured in DMEM/F12, supplemented with FBS 10% + 1% Antibiotic/Antifungal + 2.4 mM L-Glutamine + 2.4 mM pyruvate up to passage 5. Expression of pluripotent and surface marker genes was evaluated at mRNA level. Mesodermal differentiation (adipogenic, osteogenic and chondrogenic) was described. AMSCs expressed mRNA of pluripotent genes Oct4, Nanog, Sox2 and Klf4 and surface markers Cd44 , Cd90 , Cd105 and MHCII . Fibroblasts showed similar mRNA expression with the exception of Sox2 . AMSCs obtained from cougar exhibit multipotency features similar to domestic cats MSC, nevertheless, other analyses are required. AMSCs from cougar could be a source of interest for treatment of individuals that remain in captivity or arrive to wildlife rehabilitation centers., Competing Interests: Conflicts of interest: The authors have no conflict of interest to declare., (Copyright © The Author(s).)

A jaguatirica (Leopardus pardalis) é uma das espécies de felino silvestre que pouco foi investigada quanto a sua morfologia. Assim, o estudo objetivou detalhar a origem e distribuição dos ramos colaterais da aorta abdominal deste animal. Avaliou-se dois exemplares, sendo um macho e uma fêmea, jovens, provenientes de Paragominas-PA, doados ao Laboratório de Pesquisa Morfológica Animal (LaPMA) da Universidade Federal Rural da Amazônia (UFRA). O sistema arterial foi preenchido com látex pigmentado de vermelho e os cadáveres foram preservados com solução de formaldeído tamponado a 10%. A aorta abdominal do L. pardalis teve origem entre T12 e L1, sendo a artéria celíaca o primeiro ramo visceral no sentido crânio-caudal, resultando nas artérias hepática, gástrica esquerda e esplênica. A artéria mesentérica cranial surgiu como segundo ramo da aorta abdominal, originando as artérias jejunais. Na sequência localizamos artéria pancreáticoduodenal caudal, artérias ileais, artérias ileocólicas, artérias renais direita e esquerda, artérias adrenais direita e esquerda e artérias ováricas ou testiculares direita e esquerda. Parietalmente, a aorta abdominal originou em média seis ramos lombares, bem como a artéria frenicoabdominal, as artérias circunflexas ilíacas profundas e artérias ilíacas externa e interna. A aorta abdominal gerou ainda a artéria mesentérica caudal, a qual dividiu-se em artérias cólica esquerda e retal cranial. A artéria cólica esquerda seguiu cranialmente paralela ao cólon descendente irrigando-o, originando em média 18 ramos, e anastomosando-se com a artéria cólica média. A artéria retal cranial seguiu em direção caudal distribuindo oito ramos à porção final do cólon descendente e ao reto, e uniu-se com a artéria retal média. Por fim, a aorta abdominal emitiu como ramo terminal a artéria sacral mediana. A vascularização arterial abdominal desta espécie é bastante semelhante ao descrito em felinos domésticos e demais mamíferos, com diferenças quanto ao número de artérias jejunais e origem das artérias renais.