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8. Evaluating DNA barcoding for species identification and discovery in European Gracillariid moths

Author

Lopez-Vaamonde, C. (Carlos), Kirichenko, N. (Natalia), Cama, A. (Alain), Doorenweerd, C. (Camiel), Godfray, H. C. (H. Charles J.), Guiguet, A. (Antoine), Gomboc, S. (Stanislav), Huemer, P. (Peter), Landry, J.-F. (Jean-François), Laštůvka, A. (Ales), Laštůvka, Z. (Zdenek), Lee, K. M. (Kyung Min), Lees, D. C. (David C.), Mutanen, M. (Marko), van Nieukerken, E. J. (Erik J.), Segerer, A. H. (Andreas H.), Triberti, P. (Paolo), Wieser, C. (Christian), Rougerie, R. (Rodolphe), Lopez-Vaamonde, C. (Carlos), Kirichenko, N. (Natalia), Cama, A. (Alain), Doorenweerd, C. (Camiel), Godfray, H. C. (H. Charles J.), Guiguet, A. (Antoine), Gomboc, S. (Stanislav), Huemer, P. (Peter), Landry, J.-F. (Jean-François), Laštůvka, A. (Ales), Laštůvka, Z. (Zdenek), Lee, K. M. (Kyung Min), Lees, D. C. (David C.), Mutanen, M. (Marko), van Nieukerken, E. J. (Erik J.), Segerer, A. H. (Andreas H.), Triberti, P. (Paolo), Wieser, C. (Christian), and Rougerie, R. (Rodolphe)

Abstract

Gracillariidae is the most species-rich leaf-mining moth family with over 2,000 described species worldwide. In Europe, there are 263 valid named species recognized, many of which are difficult to identify using morphology only. Here we explore the use of DNA barcodes as a tool for identification and species discovery in European gracillariids. We present a barcode library including 6,791 COI sequences representing 242 of the 263 (92%) resident species. Our results indicate high congruence between morphology and barcodes with 91.3% (221/242) of European species forming monophyletic clades that can be identified accurately using barcodes alone. The remaining 8.7% represent cases of non-monophyly making their identification uncertain using barcodes. Species discrimination based on the Barcode Index Number system (BIN) was successful for 93% of species with 7% of species sharing BINs. We discovered as many as 21 undescribed candidate species, of which six were confirmed from an integrative approach; the other 15 require additional material and study to confirm preliminary evidence. Most of these new candidate species are found in mountainous regions of Mediterranean countries, the South-Eastern Alps and the Balkans, with nine candidate species found only on islands. In addition, 13 species were classified as deep conspecific lineages, comprising a total of 27 BINs with no intraspecific morphological differences found, and no known ecological differentiation. Double-digest restriction-site associated DNA sequencing (ddRAD) analysis showed strong mitonuclear discrepancy in four out of five species studied. This discordance is not explained by Wolbachia-mediated genetic sweeps. Finally, 26 species were classified as “unassessed species splits” containing 71 BINs and some involving geographical isolation or ecological specialization that will require further study to test whether they represent new cryptic species.

Published
2021

12. DNA barcode library for European Gelechiidae (Lepidoptera) suggests greatly underestimated species diversity

Author

Huemer, P. (Peter), Karsholt, O. (Ole), Aarvik, L. (Leif), Berggren, K. (Kai), Bidzilya, O. (Oleksiy), Junnilainen, J. (Jari), Landry, J.-F. (Jean-François), Mutanen, M. (Marko), Nupponen, K. (Kari), Segerer, A. (Andreas), Šumpich, J. (Jan), Wieser, C. (Christian), Wiesmair, B. (Benjamin), Hebert, P. D. (Paul D. N.), Huemer, P. (Peter), Karsholt, O. (Ole), Aarvik, L. (Leif), Berggren, K. (Kai), Bidzilya, O. (Oleksiy), Junnilainen, J. (Jari), Landry, J.-F. (Jean-François), Mutanen, M. (Marko), Nupponen, K. (Kari), Segerer, A. (Andreas), Šumpich, J. (Jan), Wieser, C. (Christian), Wiesmair, B. (Benjamin), and Hebert, P. D. (Paul D. N.)

Abstract

For the first time, a nearly complete barcode library for European Gelechiidae is provided. DNA barcode sequences (COI gene — cytochrome c oxidase 1) from 751 out of 865 nominal species, belonging to 105 genera, were successfully recovered. A total of 741 species represented by specimens with sequences ≥ 500bp and an additional ten species represented by specimens with shorter sequences were used to produce 53 NJ trees. Intraspecific barcode divergence averaged only 0.54% whereas distance to the Nearest-Neighbour species averaged 5.58%. Of these, 710 species possessed unique DNA barcodes, but 31 species could not be reliably discriminated because of barcode sharing or partial barcode overlap. Species discrimination based on the Barcode Index System (BIN) was successful for 668 out of 723 species which clustered from minimum one to maximum 22 unique BINs. Fifty-five species shared a BIN with up to four species and identification from DNA barcode data is uncertain. Finally, 65 clusters with a unique BIN remained unidentified to species level. These putative taxa, as well as 114 nominal species with more than one BIN, suggest the presence of considerable cryptic diversity, cases which should be examined in future revisionary studies.

Published
2020

13. Large geographic distance versus small DNA barcode divergence:insights from a comparison of European to South Siberian Lepidoptera

Author

Huemer, P. (Peter), Hebert, P. D. (Paul D. N.), Mutanen, M. (Marko), Wieser, C. (Christian), Wiesmai, B. (Benjamin), Hausmann, A. (Axel), Yakovlev, R. (Roman), Mös, M. (Markus), Gottsberger, B. (Brigitte), Strutzenberger, P. (Patrick), and Fiedler, K. (Konrad)

Abstract

Spanning nearly 13,000 km, the Palearctic region provides an opportunity to examine the level of geographic coverage required for a DNA barcode reference library to be effective in identifying species with broad ranges. This study examines barcode divergences between populations of 102 species of Lepidoptera from Europe and South Siberia, sites roughly 6,000 km apart. While three-quarters of these species showed divergence between their Asian and European populations, these divergence values ranged between 0–1%, distinctly less than the distance to the Nearest-Neighbor species in all but a few cases. Our results suggest that further taxonomic studies may be required for 16 species that showed either extremely low interspecific or high intraspecific variation. For example, seven species pairs showed low or no barcode divergence, but four of these cases are likely to reflect taxonomic over-splitting while the others involve species pairs that are either young or show evidence for introgression. Conversely, some of the nine species with deep intraspecific divergence at varied spatial levels may include overlooked species. Although these 16 cases require further investigation, our overall results indicate that barcode reference libraries based on records from one locality can be very effective in identifying specimens across an extensive geographic area.

Published
2018

15. ARTigo: Building an Artwork Search Engine With Games and Higher-Order Latent Semantic Analysis

Author

Wieser, C., François Bry, Bérard, A., and Lagrange, R.

Abstract

This article describes how a semantic search engine has been build from, and still is continuously improved by, a semantic analysis of the “footprints” left by players on the gaming Web platform ARTigo. The Web platform offers several Games With a Purpose (GWAPs) some of which have been specifically designed to collect the data needed for building the artwork search engine. ARTigo is a “tagging ecosystem” of games that cooperate so as to gather a wide range of information on artworks. The ARTigo ecosystem generates a folksonomy saved as 3rd-order tensor, that is a generalization of a matrix, the three orders or dimensions of which represent (1) who (2) tagged an (3) an artwork. The semantic search engine is build using a non-trivial generalization of the well-known, matrix-based, Latent Semantic Analysis (LSA) methods and algorithms. ARTigo is in service for five years and is subject to an active research constantly resulting in new developments, some of which are reported about for the first time in this article.

Published
2013
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20. Search for lightest neutralino and stau pair production in light gravitino scenarios with stau NLSP

Author

Abreu, P Adam, W Adye, T Adzic, P Alderweireld, T and Alekseev, GD Alemany, R Allmendinger, T Allport, PP and Almehed, S Amaldi, U Amato, S Anassontzis, EG Andersson, P Andreazza, A Andringa, S Antilogus, P Apel, WD and Arnoud, Y Asman, B Augustin, JE Augustinus, A Baillon, P and Bambade, P Barao, F Barbiellini, G Barbier, R and Bardin, DY Barker, G Baroncelli, A Battaglia, M and Baubillier, M Becks, KH Begalli, M Beilliere, P and Belokopytov, Y Belous, K Benvenuti, AC Berat, C and Berggren, M Bertini, D Bertrand, D Besancon, M Bianchi, F Bigi, M Bilenky, MS Bizouard, MA Bloch, D Blom, HM and Bonesini, M Bonivento, W Boonekamp, M Booth, PSL and Borgland, AW Borisov, G Bosio, C Botner, O Boudinov, E and Bouquet, B Bourdarios, C Bowcock, TJV Boyko, I and Bozovic, I Bozzo, M Branchini, P Brenke, T Brenner, RA and Bruckman, P Brunet, JM Bugge, L Buran, T and Burgsmueller, T Buschmann, P Cabrera, S Caccia, M Calvi, M Rozas, AJC Camporesi, T Canale, V Carena, F and Carroll, L Caso, C Gimenez, MVC Cattai, A Cavallo, FR and Chabaud, V Chapkin, M Charpentier, P Chaussard, L and Checchia, P Chelkov, GA Chierici, R Chliapnikov, P and Chochula, P Chorowicz, V Chudoba, J Collins, P Colomer, M Contri, R Cortina, E Cosme, G Cossutti, F Cowell, JH Crawley, HB Crennell, D Crosetti, G Maestro, JC and Czellar, S Damgaard, G Davenport, M Da Silva, W and Deghorain, A Della Ricca, G Delpierre, P Demaria, N De Angelis, A De Boer, W De Brabandere, S De Clercq, C De Lotto, B De Min, A De Paula, L Dijkstra, H Eigen, G and Ekelof, T Ekspong, G Ellert, M Elsing, M Engel, JP and Erzen, B Santo, ME Falk, E Fanourakis, G Fassouliotis, D and Fayot, J Feindt, M Fenyuk, A Ferrari, P Ferrer, A and Ferrer-Ribas, E Fichet, S Firestone, A Fischer, PA and Flagmeyer, U Foeth, H Fokitis, E Fontanelli, F Franek, B and Frodesen, AG Fruhwirth, R Fulda-Quenzer, F Fuster, J and Galloni, A Gamba, D Gamblin, S Gandelman, M Garcia, C and Garcia, J Gaspar, C Gaspar, G Gasparini, U Gavillet, P Gazis, EN Gele, D Ghodbane, N Gil, I Glege, F and Gokieli, R Golob, B Gomez-Ceballos, G Goncalves, P and Caballero, IG Gopal, G Gorn, L Gorski, M Gouz, Y and Gracco, V Grahl, J Graziani, E Green, C Grimm, HJ and Gris, P Grzelak, K Gunther, M Guy, J Hahn, F Hahn, S and Haider, S Hallgren, A Hamacher, K Harris, FJ and Hedberg, V Heising, S Hernandez, JJ Herquet, P Herr, H and Hessing, TL Heuser, JM Higon, E Holmgren, SO Holt, PJ Holthuizen, D Hoorelbeke, S Houlden, M Hrubec, J and Huet, K Hultqvist, K Jackson, JN Jacobsson, R Jalocha, P and Janik, R Jarlskog, C Jarlskog, G Jarry, P and Jean-Marie, B Johansson, EK Jonsson, P Joram, C Juillot, P Kapusta, F Karafasoulis, K Katsanevas, S Katsoufis, EC and Keranen, R Kersevan, BP Khomenko, BA Khovanski, NN and Kiiskinen, A King, B Kjaer, NJ Klapp, O Klein, H and Kluit, P Kokkinias, P Koratzinos, M Kostioukhine, V and Kourkoumelis, C Kouznetsov, O Krammer, M Kreuter, C and Kriznic, E Krstic, J Krumstein, Z Kubinec, P Kucewicz, W and Kurvinen, K Lamsa, JW Lane, DW Langefeld, P Lapin, V and Laugier, JP Lauhakangas, R Ledroit, F Lefebure, V and Leinonen, L Leisos, A Leitner, R Lenzen, G Lepeltier, V and Lesiak, T Lethuillier, M Libby, J Liko, D Lipniacka, A Lippi, I Loerstad, B Loken, JG Lopes, JH Lopez, JM and Lopez-Fernandez, R Loukas, D Lutz, P Lyons, L and MacNaughton, J Mahon, JR Maio, A Malek, A Malmgren, TGM and Malychev, V Mandl, F Marco, J Marco, R Marechal, B and Margoni, M Marin, JC Mariotti, C Markou, A and Martinez-Rivero, C Martinez-Vidal, F Garcia, SMI Masik, J and Mastroyiannopoulos, N Matorras, F Matteuzzi, C Matthiae, G Masik, J Mazzucato, F Mazzucato, M McCubbin, M and McKay, R McNulty, R McPherson, G Meroni, C Meyer, WT and Migliore, E Mirabito, L Mitaroff, WA Mjoernmark, U Moa, T Moeller, R Moenig, K Monge, MR Moreau, X and Morettini, P Morton, G Mueller, U Muenich, K Mulders, M and Mulet-Marquis, C Muresan, R Murray, WJ Muryn, B and Myatt, G Myklebust, T Naraghi, F Navarria, FL Navas, S and Nawrocki, K Negri, P Neufeld, N Neumeister, N and Nicolaidou, R Nielsen, BS Nikolenko, M Nomokonov, V and Normand, A Nygren, A Obraztsov, V Olshevski, AG Onofre, A Orava, R Orazi, G Osterberg, K Ouraou, A Paganoni, M Paiano, S Pain, R Paiva, R Palacios, J Palka, H and Papadopoulou, TD Papageorgiou, K Pape, L Parkes, C and Parodi, F Parzefall, U Passeri, A Passon, O Pegoraro, M and Peralta, L Pernicka, M Perrotta, A Petridou, C and Petrolini, A Phillips, HT Piana, G Pierre, F Pimenta, M and Piotto, E Podobnik, T Pol, ME Polok, G Poropat, P and Pozdniakov, V Privitera, P Pukhaeva, N Pullia, A and Radojicic, D Ragazzi, S Rahmani, H Rakoczy, D Ratoff, PN and Read, AL Rebecchi, P Redaelli, NG Regler, M Reid, D and Reinhardt, R Renton, PB Resvanis, LK Richard, F and Ridky, J Rinaudo, G Rohne, O Romero, A Ronchese, P and Rosenberg, EI Rosinsky, P Roudeau, P Rovelli, T and Ruhlmann-Kleider, V Ruiz, A Saarikko, H Sacquin, Y and Sadovsky, A Sajot, G Salt, J Sampsonidis, D Sannino, M and Schneider, H Schwemling, P Schwickerath, U Schyns, MAE and Scuri, F Seager, P Sedykh, Y Segar, AM Sekulin, R and Shellard, RC Sheridan, A Siebel, M Silvestre, R and Simard, L Simonetto, F Sisakian, AN Skaali, TB Smadja, G and Smirnov, N Smirnova, O Smith, GR Sopczak, A and Sosnowski, R Spassov, T Spiriti, E Sponholz, P Squarcia, S Stampfer, D Stanescu, C Stanic, S Stapnes, S and Stevenson, K Stocchi, A Strauss, J Strub, R Stugu, B and Szczekowski, M Szeptycka, M Tabarelli, T Tegenfeldt, F and Terranova, F Thomas, J Tilquin, A Timmermans, J and Tkatchev, LG Todorova, S Toet, DZ Tomaradze, A Tome, B and Tonazzo, A Tortora, L Transtromer, G Treille, D and Tristram, G Troncon, C Tsirou, A Turluer, ML Tyapkin, IA and Tzamarias, S Ueberschaer, B Ullaland, O Uvarov, V and Valenti, G Vallazza, E Van Apeldoorn, GW Van Dam, P Van Eldik, J Van Lysebetten, A Van Vulpen, I Vassilopoulos, N and Vegni, G Ventura, L Venus, W Verbeure, F Verlato, M and Vertogradov, LS Verzi, V Vilanova, D Vitale, L and Vlasov, E Vodopyanov, AS Vollmer, C Voulgaris, G Vrba, V and Wahlen, H Walck, C Wieser, C Wicke, D Wickens, JH and Wilkinson, GR Winter, M Witek, M Wolf, G Yi, J and Yushchenko, O Zaitsev, A Zalewska, A Zalewski, P and Zavrtanik, D Zevgolatakos, E Zimin, NI Zucchelli, GC and Zumerle, G DELPHI Collaboration

Abstract

Promptly decaying lightest neutralinos and long-lived staus are searched for in the context of light gravitino scenarios. It is assumed that the stau is the next to lightest supersymmetric particle (NLSP) and that the lightest neutralino is the next to NLSP (NNLSP). Data collected with the Delphi detector at centre-of-mass energies from 161 to 183 GeV are analysed. No evidence of the production of these particles is found. Hence, lower mass limits for both kinds of particles are set at 95% C.L.. The mass of gaugino-like neutralinos is found to be greater than 71.5 GeV/c(2). In the search for long-lived stau: masses less than 70.0 to 77.5 GeV/c(2) are excluded for gravitino masses from 10 to 150 eV/c(2). Combining this search with the searches for stable heavy leptons and minimal supersymmetric standard model staus a lower limit of 68.5 GeV/c(2) may be set for the stau mass independent of the mass of the gravitino.

Published
1999

30. Endothelin and Big Endothelin Concentrations in Injured Patients

Author

J. Koller, Wieser C, Puschendorf B, M. Herold, A. Pomaroli, and Peter Mair

Subjects
Adult, Male, medicine.medical_specialty, Adolescent, Endothelin-1, business.industry, Endothelins, General Medicine, Middle Aged, Endocrinology, Internal medicine, medicine, Humans, Wounds and Injuries, Female, Big endothelin 1, Protein Precursors, business, Endothelin receptor, Aged
Published
1991
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31. Intraoperative Kalorimetrie bei Aortengabelrekonstruktion.

Author

Balogh, D, Wieser, C, Mair, P, Furtwängler, W, Weimann, S, and Gruber, E

Abstract

Oxygen uptake (VO2) and carbon dioxide elimination (VCO2) can be measured with an indirect calorimeter, this method is well established in routine monitoring of ICU patients to evaluate metabolic state as a reflection of stress. In various experimental studies it was demonstrated that anaesthetics can influence whole-body metabolism. The purpose of this study was to examine whether indirect calorimetry can be used intraoperatively during routine anaesthesia and whether presumable changes in metabolism can be detected immediately. Abdominal aortic cross-clamping changes circulation, nutritional supply of the lower extremities and thus VO2 and VCO2. We therefore used this operation for our study. METHOD. Eleven patients, mean age 64 years, undergoing reconstruction of the aortic bifurcation, were studied. After premedication with piritramid and atropine, total intravenous anaesthesia (TIVA) was performed with fentanyl and midazolam after an induction with thiopental. Patients were ventilated with a Servo-Ventilator 900 D and a constant FiO2 of 0.5, without N2O. Routine monitoring consisted of ECG, pulsoximetry, CVP and continuous AP. VO2 and VCO2 were measured with a Deltatrac (Datex), and data were registered every minute. For statistical evaluation we used a Wilcoxon-Ranksum test for matched pairs, p < 0.05 was considered significant. Data from specific time (5 min after intubation, 5 min before clamping; 5, 10 and 15 min after clamping, before declamping and 5 and 10 min after declamping and at the end of surgery) were calculated. In addition to absolute values, we compared the measured VO2 and VCO2 to baseline (5 min before clamping = MP2). RESULTS. Mean operating time was 139 min +/- 37; aortic cross-clamping time for the first extremity was 38 min and 55 min for the second. As expected, there was a significant decrease in VO2 (90% of baseline) and VCO2 (75% of baseline) during aortic cross-clamping. After declamping VO2 again rose to 110% of baseline, or to 103% for the second limb. VCO2 increased to only 90% and 82%, respectively. At the end of surgery VO2 reached baseline, whereas VCO2 remains at 83%. The respiratory quotient VCO2: VO2 was markedly reduced from 0.95 +/- 0.156 to 0.73 +/- 0.06 during surgery. The Deltatrac showed every change in VO2 without delay; changes in VCO2 seem to occur somewhat retarded. DISCUSSION. Aortic cross-clamping leads to a marked decrease in VO2 and VCO2 reflecting the temporary reduction in whole-body metabolism. Declamping results in a compensatory rise, especially in VO2. VCO2 seems to increase less after declamping, perhaps due to the CO2 pool of the organism or to a change in metabolism from carbohydrate to mainly fat oxidation. The results of this study demonstrate that indirect calorimetry can easily be performed during anaesthesia and surgery. Preconditions are a non-rebreathing system without airleak, constant FiO2 < 0.6 and no use of nitrous oxide. [ABSTRACT FROM AUTHOR]

Published
1995

33. Endocrine abnormalities in severe traumatic brain injury--a cue to prognosis in severe craniocerebral trauma?

Author

Hackl, J., Gottardis, M., Wieser, Ch., Rumpl, E., Stadler, Ch., Schwarz, S., Monkayo, R., Hackl, J M, Wieser, C, and Stadler, C

Abstract

Patients with severe craniocerebral trauma (sCCT) display metabolic and endocrine changes. The question is raised whether hormonal patterns give cues to the prognosis of outcome or not. In 21 patients the function of the adrenocortical, gonadal, thyroid and human growth hormone (hGH)-insulin system was assessed. LH, FSH, TSH, prolactin and hGH were stimulated. 3 groups of patients were formed. Group I: patients in acute phase with a Glasgow Coma Score (GCS) more than 6 (group Ia) and less than 6 (group Ib). Group II: patients in transition to traumatic apallic syndrome (TAS). Group III: patients with full-blown or resolving TAS. The values of group Ia comprised low T3, T4 and testosterone, elevated insulin, normal hGH. Group Ib had hypothyroid T3 and T4 and an attenuated response of LH, TSH, prolactin and hGH to stimulation. Group III: there was seen an endocrine normalisation with elevated T4 and TBG and an altered response of hGH and prolactin to stimulation. Endocrine abnormalities were not helpful in predicting which course, either to better or to worse, a given patient would follow. [ABSTRACT FROM AUTHOR]

Published
1991
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34. Die primäre Knochenbruchheilung und ihre Störung im Röntgenbild.

Author

Wieser, C.

Abstract

Copyright of Langenbecks Archiv fuer Chirurgie is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
1965
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40. Exit-site care in Austrian peritoneal dialysis centers - A nationwide survey

Author

Kopriva-Altfahrt, G., König, P., Mündle, M., Prischl, F., Roob, J. M., Wiesholzer, M., Vychytil, A., Arneitz, K., Karner, A., Artes, R., Wolf, E., Auinger, M., Pawlak, A., Fraberger, J., Hofbauer, S., Galvan, G., Salmhofer, H., Pichler, B., Wazel, M., Gruber, M., Thonhofer, A., Hager, A., Malajner, S., Heiss, S., Braunsteiner, T., Zweifler, M., Rudnicki, M., Kogler, R., Kohlhauser, D., Moser, E., Peter Kotanko, Loibner, H., Nitz, H., Miska, H. J., Wenzel, R., Wölfler, M., Breuss, H., Hölzl, B., Oberortner, W., Schmekal, B., Riener, E. -M, Wonisch, W., Vikydal, R., Frank, B., Wieser, C., and Pokorny, K.

Subjects
Adult, Male, Adolescent, Middle Aged, Anti-Bacterial Agents, Young Adult, Catheters, Indwelling, Patient Education as Topic, Austria, Catheter-Related Infections, Health Care Surveys, Humans, Female, Practice Patterns, Physicians', Gram-Negative Bacterial Infections, Peritoneal Dialysis, Device Removal, Gram-Positive Bacterial Infections, Aged
Abstract

Catheter-associated infections markedly contribute to treatment failure in peritoneal dialysis (PD) patients. There is much controversy surrounding prophylactic strategies to prevent these infections.In this nationwide multicenter study we analyzed strategies to prevent catheter-associated infections as performed in Austrian PD centers in 2006. A questionnaire was sent to all 23 PD centers in Austria.Ten different catheter models were used in the 332 patients being treated in the 23 Austrian PD centers. Systemic antibiotics prior to catheter placement were given by 17 of the 23 PD centers (glycopeptides, n = 7; cephalosporins, n = 10). Nasal swabs were taken preoperatively by 17 PD centers; nasal Staphylococcus aureus carriers were treated prophylactically with mupirocin cream in 15 of these centers. Dressing change was routinely performed in 318 of 332 chronic PD patients (nonocclusive film dressing, n = 58; gauze dressing, n = 260). Disinfectants for chronic exit-site care included povidone iodine (n = 155), sodium hypochlorite (n = 31), povidone iodine + sodium hypochlorite together (n = 102), and octenidine dihydrochloride/phenoxyethanol (n = 17). Water + non-disinfectant soap or 0.9% sodium chloride was administered as a cleansing agent to the exit site by 27 patients. Routine S. aureus screening (nasal and/or exit-site swabs) in chronic PD patients was performed in 12 PD centers; carriers were treated with mupirocin cream in 11 of these centers. Dialysis staff members were screened for S. aureus in 8 PD centers and spouses were screened for S. aureus in 5 PD centers. The overall exit-site infection rate was 1 episode/43.9 patient-months, tunnel infection rate was 1 episode/88.9 patient-months, and peritonitis rate was 1 episode/51.0 patient-months. Patients of centers that have installed a prophylaxis protocol for treating S. aureus carriers had lower mean infection rates compared with those not using such a protocol.Various individual prophylactic strategies are used to prevent catheter-associated infections in Austrian PD centers. Infection rates are within the range reported in the literature. There is still scope for improvement in some centers (e.g., by establishing a prophylaxis protocol).

47. Factor structure and reliability of the Italian adaptation of the Hypomania Check List-32, second revision (HCL-32-R2)

Author

Matteo Martino, Concetta De Pasquale, Giorgio Consoli, Domenico De Berardis, Fulvio Bedani, Michele Fornaro, Mai Elassy, Christian Wieser, Febronia Dugo, Carlo Ignazio Cattaneo, Sergio Mungo, Monica Mazza, Felice Iasevoli, Vito Fabio Paternò, Maria Chiara Pino, Giampaolo Perna, Giovanni Martinotti, Francesca Lo Monaco, Valerio Selle, Livia Avvisati, Emanuela D׳Angelo, Antonio Ventriglio, Andrea de Bartolomeis, Luisa Indelicato, Sergio Tartaglione, Anna Romano, Alessandro Del Debbio, Jules Angst, Alessandro Valchera, Carmine Tomasetti, Ann Sarah Koshy, Ettore Favaretto, Fornaro, Michele, De Berardis, D, Mazza, M, Pino, M, Favaretto, E, Bedani, F, Wieser, C, Indelicato, L, Paternò, Vf, Lo Monaco, F, Dugo, F, Ventriglio, A, Mungo, S, Selle, V, Valchera, A, Elassy, M, Martinotti, G, DE BARTOLOMEIS, Andrea, Iasevoli, Felice, Tomasetti, Carmine, Avvisati, L, Tartaglione, S, Perna, G, Cattaneo, Ci, Consoli, G, Romano, A, Del Debbio, A, Martino, M, Angelo E, D', De Pasquale, C, Koshy, A, and Angst, J.

Subjects
Adult, Male, 34-item, medicine.medical_specialty, Bipolar Disorder, Italian, Adolescent, Psychometrics, (HCL-32-R2), Comorbidity, Young Adult, Cronbach's alpha, Predictive Value of Tests, Recall bias, Positive predicative value, Hypomania Check-List-32, second revision (HCL-32-R2), 34-item, Italian, Depression, Bipolar disorder, 80 and over, medicine, Humans, Bipolar disorder, Medical diagnosis, Psychiatry, Check-List-32 second revision, Depression (differential diagnoses), Aged, Aged, 80 and over, Psychiatric Status Rating Scales, Depressive Disorder, Major, Depressive Disorder, Depression, Major, Reproducibility of Results, Hypomania, Diagnostic and Statistical Manual of Mental Disorders, Female, Italy, Middle Aged, medicine.disease, Psychiatry and Mental health, Clinical Psychology, Major depressive disorder, medicine.symptom, Psychology, Clinical psychology
Abstract

Objective To assess the psychometric properties of the Italian adaptation of the Hypomania-Check-List 32-item, second revision (HCL-32-R2) for the detection of bipolarity in major depressive disorder (MDD) treatment-seeking outpatients. Methods A back-to-back Italian adaption of the “Bipolar Disorders: Improving Diagnosis, Guidance, and Education” English module of the HCL-32-R2 was administered between March 2013 and October 2014 across twelve collaborating sites in Italy. Diagnostic and Statistical Manual Fourth edition (DSM-IV) diagnoses were made adopting the mini-international neuropsychiatric interview, using bipolar disorder (BD) patients as controls. Results In our sample ( n =441, of whom, BD-I=68; BD-II=117; MDD=256), using a cut-off of 14 allowed the HCL-32-R2 to discriminate DSM-IV-defined MDD patients between “true unipolar” (HCL-32-R2 − ) and “sub-threshold bipolar depression” (HCL-32-R2 + ) with sensitivity=89% and specificity=79%. Area under the curve was .888; positive and negative predictive values were 75.34% and 90.99% respectively. Owing to clinical interpretability considerations and consistency with previous adaptations of the HCL-32, a two-factor solution (F1=“ hyperactive / elated ” vs. F2=“ irritable / distractible / impulsive ”) was preferred using exploratory and confirmatory factor analyses, whereas items n.33 (“ I gamble more ”) and n.34 (“ I eat more ”) introduced in the R2 version of the scale slightly loaded onto F2 and F1 respectively. Cronbach׳s α=.88 for F1 and .71 for F2. Limitations No cross-validation with any additional validated screening tool; treatment-seeking outpatient sample; recall bias; no systematic evaluation of eventual medical/psychiatric comorbidities, current/lifetime pharmacological history, neither record of severity of current MDE. Conclusions Our results seem to indicate fair accuracy of HCL-32 as a screening instrument for BD, though replication studies are warranted.

Published
2015

49. A DNA barcode library of Austrian geometridae (Lepidoptera) reveals high potential for DNA-based species identification.

Author

Schattanek-Wiesmair B, Huemer P, Wieser C, Stark W, Hausmann A, Koblmüller S, and Sefc KM

Subjects
Animals, DNA Barcoding, Taxonomic methods, Austria, Ecosystem, Biodiversity, DNA, Lepidoptera genetics, Moths genetics
Abstract

Situated in the Eastern section of the European Alps, Austria encompasses a great diversity of different habitat types, ranging from alpine to lowland Pannonian ecosystems, and a correspondingly high level of species diversity, some of which has been addressed in various DNA barcoding projects. Here, we report a DNA barcode library of all the 476 species of Geometridae (Lepidoptera) that have been recorded in Austria. As far as possible, species were sampled from different Austrian regions in order to capture intraspecific genetic variation. In total, 2500 DNA barcode sequences, representing 438 species, were generated in this study. For complete coverage of Austrian geometrid species in the subsequent analyses, the dataset was supplemented with DNA barcodes from specimens of non-Austrian origin. Species delimitations by ASAP, BIN and bPTP methods yielded 465, 510 and 948 molecular operational taxonomic units, respectively. Congruency of BIN and ASAP partitions with morphospecies assignments was reasonably high (85% of morphospecies in unique partitions), whereas bPTP appeared to overestimate the number of taxonomic units. The study furthermore identified taxonomically relevant cases of morphospecies splitting and sharing in the molecular partitions. We conclude that DNA barcoding and sequence analysis revealed a high potential for accurate DNA-based identification of the Austrian Geometridae species. Additionally, the study provides an updated checklist of the geometrid moths of Austria., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Schattanek-Wiesmair et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Published
2024
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50. First Nationwide Monitoring Program for the Detection of Potentially Invasive Mosquito Species in Austria.

Author

Bakran-Lebl K, Pree S, Brenner T, Daroglou E, Eigner B, Griesbacher A, Gunczy J, Hufnagl P, Jäger S, Jerrentrup H, Klocker L, Paill W, Petermann JS, Barogh BS, Schwerte T, Suchentrunk C, Wieser C, Wortha LN, Zechmeister T, Zezula D, Zimmermann K, Zittra C, Allerberger F, and Fuehrer HP

Abstract

In Austria, only fragmented information on the occurrence of alien and potentially invasive mosquito species exists. The aim of this study is a nationwide overview on the situation of those mosquitoes in Austria. Using a nationwide uniform protocol for the first time, mosquito eggs were sampled with ovitraps at 45 locations in Austria at weekly intervals from May to October 2020. The sampled eggs were counted and the species were identified by genetic analysis. The Asian tiger mosquito Aedes albopictus was found at two sites, once in Tyrol, where this species has been reported before, and for the first time in the province of Lower Austria, at a motorway rest stop. The Asian bush mosquito Aedes japonicus was widespread in Austria. It was found in all provinces and was the most abundant species in the ovitraps by far. Aedes japonicus was more abundant in the South than in the North and more eggs were found in habitats with artificial surfaces than in (semi-) natural areas. Further, the number of Ae. japonicus eggs increased with higher ambient temperature and decreased with higher wind speed. The results of this study will contribute to a better estimation of the risk of mosquito-borne disease in Austria and will be a useful baseline for a future documentation of changes in the distribution of those species.

Published
2022
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