1. Characterization of 29 polymorphic microsatellite markers developed by genomic screening of Sumatran rhinoceros (Dicerorhinus sumatrensis)
- Author
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Jessica R. Brandt, Sinta H. Saidah, Kai Zhao, Yasuko Ishida, Isabella Apriyana, Oliver A. Ryder, Widodo Ramono, Herawati Sudoyo, Helena Suryadi, Peter J. Van Coeverden de Groot, and Alfred L. Roca
- Subjects
Dicerorhinus sumatrensis ,Short tandem repeats ,Non-invasive sampling ,Medicine ,Biology (General) ,QH301-705.5 ,Science (General) ,Q1-390 - Abstract
Abstract Objective The Sumatran rhinoceros is critically endangered, with fewer than 100 individuals surviving across its current range. Accurate census estimates of the remaining populations are essential for development and implementation of conservation plans. In order to enable molecular censusing, we here develop microsatellite markers with amplicon sizes of short length, appropriate for non-invasive fecal sampling. Results Due to limited sample quantity and potential lack of genome-wide diversity, Illumina sequence reads were generated from two Sumatran rhinoceros samples. Genomic screening identified reads with short tandem repeats and loci that were polymorphic within the dataset. Twenty-nine novel polymorphic microsatellite markers were characterized (A = 2.4; H O = 0.30). These were sufficient to distinguish among individuals (P ID
- Published
- 2021
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