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35 results on '"Wickramasinghe, S.R."'

4. List of contributors

Author

Algieri, C., primary, Amelio, A., additional, Basile, A., additional, Brazinha, C., additional, Calabro, V., additional, Capocelli, M., additional, Cardoso, V.L., additional, Cassano, A., additional, Castro-Muñoz, R., additional, Chakraborty, S., additional, Chen, Y., additional, Cheruvu, S., additional, Coelhoso, I.M., additional, Conidi, C., additional, Crespo, J.G., additional, Curcio, S., additional, Dehghani, Z., additional, De Luca, G., additional, Di Paola, L., additional, Donato, L., additional, Figoli, A., additional, Galanakis, C.M., additional, Galiano, F., additional, Garofalo, A., additional, Ghasemzadeh, K., additional, Gijon-Felix, R., additional, Hu, Z., additional, Iovane, P., additional, Jiang, C., additional, Jönsson, A.-S., additional, Kalil Duarte, L.H., additional, Lee, H.J., additional, Lopresto, C., additional, Luis, P., additional, Malmali, M., additional, Marino, T., additional, Meireles, I.T., additional, Migliori, M., additional, Molino, A., additional, Nategh, M., additional, Orticello, G., additional, Piemonte, V., additional, Qian, X., additional, Rahimpour, M.R., additional, Rego De Oliveira, S.H., additional, Reis, M.H.M., additional, Sadati Tilebon, S.M., additional, Saha, K., additional, Salvi, E., additional, Seyed Dorraji, M.S., additional, Steiner, D., additional, Van der Bruggen, B., additional, Van Ginkel, S., additional, Vatanpour, V., additional, Verardi, A., additional, Wang, Y., additional, Wei, X., additional, Wickramasinghe, S.R., additional, Xu, C., additional, Xu, T., additional, and Zhang, X., additional

Published
2016
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20. Kinetic, inhibition and structural studies on 3-oxoacyl-ACP reductase from Plasmodium falciparum, a key enzyme in fatty acid biosynthesis

Author

Wickramasinghe, S.R., Inglis, K.A., Urch, J.E., Müller, S., van Aalten, D.M., and Fairlamb, A.H.

Subjects
QR180
Abstract

Type II fatty acid biosynthesis represents an attractive target for the discovery of new antimalarial drugs. Previous studies have identified malarial ENR (enoyl acyl-carrier-protein reductase, or FabI) as the target for the antiseptic triclosan. In the present paper, we report the biochemical properties and 1.5 Å (1 Å=0.1 nm) crystal structure of OAR (3-oxoacyl acyl-carrier-protein reductase, or FabG), the second reductive step in fatty acid biosynthesis and its inhibition by hexachlorophene. Under optimal conditions of pH and ionic strength, Plasmodium falciparum OAR displays kinetic properties similar to those of OAR from bacteria or plants. Activity with NADH is Escherichia coli OAR–NADP+ complex. A cluster of positively charged residues is positioned at the entrance to the active site, consistent with the proposed recognition site for the physiological substrate (3-oxoacyl-acyl-carrier protein) in E coli OAR. The antibacterial and anthelminthic agent hexachlorophene is a potent inhibitor of OAR (IC50 2.05 μM) displaying non-linear competitive inhibition with respect to NADPH. Hexachlorophene (EC50 6.2 μM) and analogues such as bithionol also have antimalarial activity in vitro, suggesting they might be useful leads for further development.

Published
2006

35. Back-extraction Of Pu 4 +  From 20% Tributyl Phosphate – 20% Mixed Trialkyl Phosphine Oxides/kerosene In The Presence Of.

Author

Han, Binbing, Wu, Qiulin, Zhu, Yongjun, and Wickramasinghe, S.R.

Subjects
EXTRACTION (Chemistry), PETROLEUM products, PHOSPHORUS compounds, ORGANOPHOSPHORUS compounds, KEROSENE, OXALIC acid
Abstract

Back-extraction of Pu 4 +  from a mixture of 20% tributyl phosphate (TBP) and 20% mixed trialkyl phosphine oxides (TRPO) in kerosene in the presence of UO 2+ 2 was studied. The back-extractants investigated may be divided into three groups: carboxylic acids and salts, amino polycarboxylates, and phosphonic acid. The distribution coefficients of both Pu 4 +  and UO 2+ 2 using a number of different back-extractants were measured and compared. The results obtained suggest that the only practical back-extractants are carboxylic acids. Among the carboxylic acids tested, oxalic acid is suitable when the UO 2+ 2 concentration in the organic phase is less than 2 g/L. For UO 2+ 2 concentrations between 2 and 10 g/L, oxalic acid-nitric acid mixtures may be used. For UO 2+ 2 concentrations greater than 10 g/L, the only practical back-extractant is glycolic acid. The results obtained here may be used to further develop a new process for separation of Pu 4 +  and UO 2+ 2 from TBP-TRPO/kerosene mixture. [ABSTRACT FROM AUTHOR]

Published
2005
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