562 results on '"Wichels A"'
Search Results
2. The travelling particles: community dynamics of biofilms on microplastics transferred along a salinity gradient
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Song, Jessica, Beule, Lukas, Jongmans-Hochschulz, Elanor, Wichels, Antje, and Gerdts, Gunnar
- Published
- 2022
- Full Text
- View/download PDF
3. Shell Disease Syndrome Is Associated with Reduced and Shifted Epibacterial Diversity on the Carapace of the Crustacean Cancer pagurus
- Author
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Nils Bergen, Philipp Krämer, Julia Romberg, Antje Wichels, Gabriele Gerlach, and Thorsten Brinkhoff
- Subjects
Crustacea ,microbiome composition ,shell disease ,Aquimarina ,Rhodobacteraceae ,microbe-host interaction ,Microbiology ,QR1-502 - Abstract
ABSTRACT Cancer pagurus is highly susceptible to shell disease syndrome. However, little is known about concomitant changes in the epibacterial community. We compared the bacterial communities of black spot affected and nonaffected areas of the carapace by amplicon sequencing of 16S rRNA genes and 16S rRNA. Within each spot, bacterial communities of affected areas were less diverse compared to communities from nonaffected areas. Communities of different affected spots were, however, more divergent from each other, compared to those of different nonaffected areas. This indicates a reduced and shifted microbial community composition caused by the black spot disease. Different communities found in black spots likely indicate different stages of the disease. In affected areas, Flavobacteriaceae rose to one of the most abundant and active families due to the increase of Aquimarina spp., suggesting a significant role in shell disease syndrome. We isolated 75 bacterial strains from diseased and healthy areas, which are primarily affiliated with Proteobacteria and Bacteroidetes, reflecting the dominant phyla detected by amplicon sequencing. The ability to degrade chitin was mainly found for Gammaproteobacteria and Aquimarina spp. within the Flavobacteriia, while the ability to use N-acetylglucosamine, the monomer of the polysaccharide chitin, was observed for most isolates, including many Alphaproteobacteria. One-third of the isolates, including most Aquimarina spp., showed antagonistic properties, indicating a high potential for interactions between the bacterial populations. The combination of bacterial community analysis and the physiological properties of the isolates provided insights into a functional complex epibacterial community on the carapace of C. pagurus. IMPORTANCE In recent years, shell disease syndrome has been detected for several ecologically and economically important crustacean species. Large proportions of populations are affected, e.g., >60% of the widely distributed species Cancer pagurus in different North Sea areas. Bacteria play a significant role in the development of different forms of shell disease, all characterized by microbial chitinolytic degradation of the outer shell. By comparing the bacterial communities of healthy and diseased areas of the shell of C. pagurus, we demonstrated that the disease causes a reduced bacterial diversity within affected areas, a phenomenon co-occurring also with many other diseases. Furthermore, the community composition dramatically changed with some taxa rising to high relative abundances and showing increased activity, indicating strong participation in shell disease. Characterization of bacterial isolates obtained from affected and nonaffected spots provided deeper insights into their physiological properties and thus the possible role within the microbiome.
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- 2022
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4. Comparative Omics and Trait Analyses of Marine Pseudoalteromonas Phages Advance the Phage OTU Concept
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Duhaime, Melissa B, Solonenko, Natalie, Roux, Simon, Verberkmoes, Nathan C, Wichels, Antje, and Sullivan, Matthew B
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Microbiology ,Biological Sciences ,Ecology ,Genetics ,Infectious Diseases ,2.2 Factors relating to the physical environment ,Aetiology ,Infection ,phage ,marine microbiology ,particle-associated ,comparative genomics ,Pseudoalteromonas ,Environmental Science and Management ,Soil Sciences ,Medical microbiology - Abstract
Viruses influence the ecology and evolutionary trajectory of microbial communities. Yet our understanding of their roles in ecosystems is limited by the paucity of model systems available for hypothesis generation and testing. Further, virology is limited by the lack of a broadly accepted conceptual framework to classify viral diversity into evolutionary and ecologically cohesive units. Here, we introduce genomes, structural proteomes, and quantitative host range data for eight Pseudoalteromonas phages isolated from Helgoland (North Sea, Germany) and use these data to advance a genome-based viral operational taxonomic unit (OTU) definition. These viruses represent five new genera and inform 498 unaffiliated or unannotated protein clusters (PCs) from global virus metagenomes. In a comparison of previously sequenced Pseudoalteromonas phage isolates (n = 7) and predicted prophages (n = 31), the eight phages are unique. They share a genus with only one other isolate, Pseudoalteromonas podophage RIO-1 (East Sea, South Korea) and two Pseudoalteromonas prophages. Mass-spectrometry of purified viral particles identified 12-20 structural proteins per phage. When combined with 3-D structural predictions, these data led to the functional characterization of five previously unidentified major capsid proteins. Protein functional predictions revealed mechanisms for hijacking host metabolism and resources. Further, they uncovered a hybrid sipho-myovirus that encodes genes for Mu-like infection rarely described in ocean systems. Finally, we used these data to evaluate a recently introduced definition for virus populations that requires members of the same population to have >95% average nucleotide identity across at least 80% of their genes. Using physiological traits and genomics, we proposed a conceptual model for a viral OTU definition that captures evolutionarily cohesive and ecologically distinct units. In this trait-based framework, sensitive hosts are considered viral niches, while host ranges and infection efficiencies are tracked as viral traits. Quantitative host range assays revealed conserved traits within virus OTUs that break down between OTUs, suggesting the defined units capture niche and fitness differentiation. Together these analyses provide a foundation for model system-based hypothesis testing that will improve our understanding of marine copiotrophs, as well as phage-host interactions on the ocean particles and aggregates where Pseudoalteromonas thrive.
- Published
- 2017
5. Diatom fucan polysaccharide precipitates carbon during algal blooms
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Silvia Vidal-Melgosa, Andreas Sichert, T. Ben Francis, Daniel Bartosik, Jutta Niggemann, Antje Wichels, William G. T. Willats, Bernhard M. Fuchs, Hanno Teeling, Dörte Becher, Thomas Schweder, Rudolf Amann, and Jan-Hendrik Hehemann
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Science - Abstract
The fate of ocean carbon is determined by the balance between primary productivity and heterotrophic breakdown of that photosynthate. Here the authors show that diatoms produce a polysaccharide that resists bacterial degradation, accumulates, aggregates and stores carbon during spring blooms.
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- 2021
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6. Genomic characterization of filamentous phage vB_VpaI_VP-3218, an inducible prophage of Vibrio parahaemolyticus
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Garin-Fernandez, Ale, Glöckner, Frank Oliver, and Wichels, Antje
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- 2020
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7. Looking for the hidden: Characterization of lysogenic phages in potential pathogenic Vibrio species from the North Sea
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Garin-Fernandez, Ale and Wichels, Antje
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- 2020
- Full Text
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8. The ocean sampling day consortium
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Kopf, Anna, Bicak, Mesude, Kottmann, Renzo, Schnetzer, Julia, Kostadinov, Ivaylo, Lehmann, Katja, Fernandez-Guerra, Antonio, Jeanthon, Christian, Rahav, Eyal, Ullrich, Matthias, Wichels, Antje, Gerdts, Gunnar, Polymenakou, Paraskevi, Kotoulas, Giorgos, Siam, Rania, Abdallah, Rehab Z, Sonnenschein, Eva C, Cariou, Thierry, O’Gara, Fergal, Jackson, Stephen, Orlic, Sandi, Steinke, Michael, Busch, Julia, Duarte, Bernardo, Caçador, Isabel, Canning-Clode, João, Bobrova, Oleksandra, Marteinsson, Viggo, Reynisson, Eyjolfur, Loureiro, Clara Magalhães, Luna, Gian Marco, Quero, Grazia Marina, Löscher, Carolin R, Kremp, Anke, DeLorenzo, Marie E, Øvreås, Lise, Tolman, Jennifer, LaRoche, Julie, Penna, Antonella, Frischer, Marc, Davis, Timothy, Katherine, Barker, Meyer, Christopher P, Ramos, Sandra, Magalhães, Catarina, Jude-Lemeilleur, Florence, Aguirre-Macedo, Ma Leopoldina, Wang, Shiao, Poulton, Nicole, Jones, Scott, Collin, Rachel, Fuhrman, Jed A, Conan, Pascal, Alonso, Cecilia, Stambler, Noga, Goodwin, Kelly, Yakimov, Michael M, Baltar, Federico, Bodrossy, Levente, Van De Kamp, Jodie, Frampton, Dion MF, Ostrowski, Martin, Van Ruth, Paul, Malthouse, Paul, Claus, Simon, Deneudt, Klaas, Mortelmans, Jonas, Pitois, Sophie, Wallom, David, Salter, Ian, Costa, Rodrigo, Schroeder, Declan C, Kandil, Mahrous M, Amaral, Valentina, Biancalana, Florencia, Santana, Rafael, Pedrotti, Maria Luiza, Yoshida, Takashi, Ogata, Hiroyuki, Ingleton, Tim, Munnik, Kate, Rodriguez-Ezpeleta, Naiara, Berteaux-Lecellier, Veronique, Wecker, Patricia, Cancio, Ibon, Vaulot, Daniel, Bienhold, Christina, Ghazal, Hassan, Chaouni, Bouchra, Essayeh, Soumya, Ettamimi, Sara, Zaid, El Houcine, Boukhatem, Noureddine, Bouali, Abderrahim, Chahboune, Rajaa, Barrijal, Said, Timinouni, Mohammed, El Otmani, Fatima, Bennani, Mohamed, and Mea, Marianna
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Microbiology ,Oceanography ,Biological Sciences ,Earth Sciences ,Life Below Water ,Biodiversity ,Database Management Systems ,Marine Biology ,Metagenomics ,Oceans and Seas ,Ocean sampling day ,OSD ,Genomics ,Health Index ,Bacteria ,Microorganism ,Marine ,Micro B3 ,Standards - Abstract
Ocean Sampling Day was initiated by the EU-funded Micro B3 (Marine Microbial Biodiversity, Bioinformatics, Biotechnology) project to obtain a snapshot of the marine microbial biodiversity and function of the world's oceans. It is a simultaneous global mega-sequencing campaign aiming to generate the largest standardized microbial data set in a single day. This will be achievable only through the coordinated efforts of an Ocean Sampling Day Consortium, supportive partnerships and networks between sites. This commentary outlines the establishment, function and aims of the Consortium and describes our vision for a sustainable study of marine microbial communities and their embedded functional traits.
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- 2015
9. Diatom fucan polysaccharide precipitates carbon during algal blooms
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Vidal-Melgosa, Silvia, Sichert, Andreas, Francis, T. Ben, Bartosik, Daniel, Niggemann, Jutta, Wichels, Antje, Willats, William G. T., Fuchs, Bernhard M., Teeling, Hanno, Becher, Dörte, Schweder, Thomas, Amann, Rudolf, and Hehemann, Jan-Hendrik
- Published
- 2021
- Full Text
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10. The founding charter of the Genomic Observatories Network
- Author
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Davies, Neil, Field, Dawn, Amaral-Zettler, Linda, Clark, Melody S, Deck, John, Drummond, Alexei, Faith, Daniel P, Geller, Jonathan, Gilbert, Jack, Glöckner, Frank Oliver, Hirsch, Penny R, Leong, Jo-Ann, Meyer, Chris, Obst, Matthias, Planes, Serge, Scholin, Chris, Vogler, Alfried P, Gates, Ruth D, Toonen, Rob, Berteaux-Lecellier, Véronique, Barbier, Michèle, Barker, Katherine, Bertilsson, Stefan, Bicak, Mesude, Bietz, Matthew J, Bobe, Jason, Bodrossy, Levente, Borja, Angel, Coddington, Jonathan, Fuhrman, Jed, Gerdts, Gunnar, Gillespie, Rosemary, Goodwin, Kelly, Hanson, Paul C, Hero, Jean-Marc, Hoekman, David, Jansson, Janet, Jeanthon, Christian, Kao, Rebecca, Klindworth, Anna, Knight, Rob, Kottmann, Renzo, Koo, Michelle S, Kotoulas, Georgios, Lowe, Andrew J, Marteinsson, Viggó Thór, Meyer, Folker, Morrison, Norman, Myrold, David D, Pafilis, Evangelos, Parker, Stephanie, Parnell, John Jacob, Polymenakou, Paraskevi N, Ratnasingham, Sujeevan, Roderick, George K, Rodriguez-Ezpeleta, Naiara, Schonrogge, Karsten, Simon, Nathalie, Valette-Silver, Nathalie J, Springer, Yuri P, Stone, Graham N, Stones-Havas, Steve, Sansone, Susanna-Assunta, Thibault, Kate M, Wecker, Patricia, Wichels, Antje, Wooley, John C, Yahara, Tetsukazu, Zingone, Adriana, and GOs-COS
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Biodiversity ,Genomics ,Biocode ,Earth observations ,GOs-COS - Abstract
The co-authors of this paper hereby state their intention to work together to launch the Genomic Observatories Network (GOs Network) for which this document will serve as its Founding Charter. We define a Genomic Observatory as an ecosystem and/or site subject to long-term scientific research, including (but not limited to) the sustained study of genomic biodiversity from single-celled microbes to multicellular organisms.An international group of 64 scientists first published the call for a global network of Genomic Observatories in January 2012. The vision for such a network was expanded in a subsequent paper and developed over a series of meetings in Bremen (Germany), Shenzhen (China), Moorea (French Polynesia), Oxford (UK), Pacific Grove (California, USA), Washington (DC, USA), and London (UK). While this community-building process continues, here we express our mutual intent to establish the GOs Network formally, and to describe our shared vision for its future. The views expressed here are ours alone as individual scientists, and do not necessarily represent those of the institutions with which we are affiliated.
- Published
- 2014
11. Mycoplankton Biome Structure and Assemblage Processes Differ Along a Transect From the Elbe River Down to the River Plume and the Adjacent Marine Waters
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Yanyan Yang, Stefanos Banos, Gunnar Gerdts, Antje Wichels, and Marlis Reich
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aquatic fungi ,estuary ,QPE analysis ,salinity gradient ,Chytridiomycota ,shallow freshwater ,Microbiology ,QR1-502 - Abstract
Rivers are transport systems and supply adjacent ecosystems with nutrients. They also serve human well-being, for example as a source of food. Microorganism biodiversity is an important parameter for the ecological balance of river ecosystems. Despite the knowledge that fungi are key players in freshwater nutrient cycling and food webs, data on planktonic fungi of streams with higher stream order are scarce. This study aims to fill this knowledge gap by a fungi-specific 18S ribosomal RNA (rRNA) gene tag sequencing approach, investigating mycoplankton diversity in the Elbe River along a transect from shallow freshwater, to the estuary and river plume down to the adjacent marine waters (sections of seventh stream order number). Using multivariate analyses and the quantitative process estimates (QPEs) method, questions (i) of how mycoplankton communities as part of the river continuum change along the transect, (ii) what factors, spatial and environmental, play a role, and (iii) what assembly processes, such as selection or dispersion, operate along the transect, were addressed. The partitioning of mycoplankton communities into three significant distant biomes was mainly driven by local environmental conditions that were partly under spatial control. The assembly processes underlying the biomes also differed significantly. Thus, variable selection dominated the upstream sections, while undominated processes like ecological drift dominated the sections close to the river mouth and beyond. Dispersal played a minor role. The results suggest that the ecological versatility of the mycoplankton communities changes along the transect as response, for example, to a drastic change from an autotrophic to a heterotrophic system caused by an abrupt increase in the river depth. Furthermore, a significant salinity-dependent occurrence of diverse basal fungal groups was observed, with no clade found exclusively in marine waters. These results provide an important framework to help understand patterns of riverine mycoplankton communities and serve as basis for a further in-depth work so that fungi, as an important ecological organism group, can be integrated into models of, e.g., usage-balance considerations of rivers.
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- 2021
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12. Mature biofilm communities on synthetic polymers in seawater - Specific or general?
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Kirstein, Inga V., Wichels, Antje, Krohne, Georg, and Gerdts, Gunnar
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- 2018
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13. The North Sea goes viral: Occurrence and distribution of North Sea bacteriophages
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Garin-Fernandez, Ale, Pereira-Flores, Emiliano, Glöckner, Frank Oliver, and Wichels, Antje
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- 2018
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14. Bacterial community succession in response to dissolved organic matter released from live jellyfish
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Hao, Wenjin, Wichels, Antje, Fuchs, Bernhardt, Tang, Xuexi, and Gerdts, Gunnar
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- 2019
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15. Bacterial communities associated with scyphomedusae at Helgoland Roads
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Hao, Wenjin, Gerdts, Gunnar, Holst, Sabine, and Wichels, Antje
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- 2019
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16. Seasonal Dynamics of Pelagic Mycoplanktonic Communities: Interplay of Taxon Abundance, Temporal Occurrence, and Biotic Interactions
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Stefanos Banos, Deisy Morselli Gysi, Tim Richter-Heitmann, Frank Oliver Glöckner, Maarten Boersma, Karen H. Wiltshire, Gunnar Gerdts, Antje Wichels, and Marlis Reich
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phytoplankton ,zooplankton ,marine fungi ,food web structure ,microbial loop ,pattern ,Microbiology ,QR1-502 - Abstract
Marine fungi are an important component of pelagic planktonic communities. However, it is not yet clear how individual fungal taxa are integrated in marine processes of the microbial loop and food webs. Most likely, biotic interactions play a major role in shaping the fungal community structure. Thus, the aim of our work was to identify possible biotic interactions of mycoplankton with phytoplankton and zooplankton groups and among fungi, and to investigate whether there is coherence between interactions and the dynamics, abundance and temporal occurrence of individual fungal OTUs. Marine surface water was sampled weekly over the course of 1 year, in the vicinity of the island of Helgoland in the German Bight (North Sea). The mycoplankton community was analyzed using 18S rRNA gene tag-sequencing and the identified dynamics were correlated to environmental data including phytoplankton, zooplankton, and abiotic factors. Finally, co-occurrence patterns of fungal taxa were detected with network analyses based on weighted topological overlaps (wTO). Of all abundant and persistent OTUs, 77% showed no biotic relations suggesting a saprotrophic lifestyle. Of all other fungal OTUs, nearly the half (44%) had at least one significant negative relationship, especially with zooplankton and other fungi, or to a lesser extent with phytoplankton. These findings suggest that mycoplankton OTUs are embedded into marine food web chains via highly complex and manifold relationships such as parasitism, predation, grazing, or allelopathy. Furthermore, about one third of all rare OTUs were part of a dense fungal co-occurrence network probably stabilizing the fungal community against environmental changes and acting as functional guilds or being involved in fungal cross-feeding. Placed in an ecological context, strong antagonistic relationships of the mycoplankton community with other components of the plankton suggest that: (i) there is a top-down control by fungi on zooplankton and phytoplankton; (ii) fungi serve as a food source for zooplankton and thereby transfer nutrients and organic material; (iii) the dynamics of fungi harmful to other plankton groups are controlled by antagonistic fungal taxa.
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- 2020
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17. A potencialidade das redes sociais na promoção de destinos turísticos: O caso de Tenerife
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S. Wichels
- Subjects
comunicação de destino turístico ,redes sociais ,turismo 2:0 ,estratégia de comunicação online em turismo ,Espanha ,turismo de Tenerife ,Geography (General) ,G1-922 ,Social sciences (General) ,H1-99 - Abstract
As redes sociais, poderosas plataformas de relacionamento, captação e fidelização de clientes, nomeadamente o TripAdvisor, o Facebook e o Instagram ou a Blogosfera, estão a influenciar novos comportamentos do consumidor, obrigando a novas práticas em comunicação turística. Frente a esta realidade, as estratégias e ferramentas de comunicação e marketing tradicionais dos destinos turísticos mostram-se menos efetivas e a mudança envolve não só o como e quando se comunica, mas também o quê. Neste artigo analisamos, enquanto estudo de caso, a estratégia de comunicação e redes sociais de Tenerife. Como estará a reagir um dos destinos mais consolidados do mundo, à mudança de paradigma em comunicação turística?
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- 2018
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18. Methane distribution and oxidation around the Lena Delta in summer 2013
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I. Bussmann, S. Hackbusch, P. Schaal, and A. Wichels
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Ecology ,QH540-549.5 ,Life ,QH501-531 ,Geology ,QE1-996.5 - Abstract
The Lena River is one of the largest Russian rivers draining into the Laptev Sea. The predicted increases in global temperatures are expected to cause the permafrost areas surrounding the Lena Delta to melt at increasing rates. This melting will result in high amounts of methane reaching the waters of the Lena and the adjacent Laptev Sea. The only biological sink that can lower methane concentrations within this system is methane oxidation by methanotrophic bacteria. However, the polar estuary of the Lena River, due to its strong fluctuations in salinity and temperature, is a challenging environment for bacteria. We determined the activity and abundance of aerobic methanotrophic bacteria by a tracer method and by the quantitative polymerase chain reaction. We described the methanotrophic population with a molecular fingerprinting method (monooxygenase intergenic spacer analysis), as well as the methane distribution (via a headspace method) and other abiotic parameters, in the Lena Delta in September 2013. The median methane concentrations were 22 nmol L−1 for riverine water (salinity (S) −1 for mixed water (5 S −1 for polar water (S > 20). The Lena River was not the source of methane in surface water, and the methane concentrations of the bottom water were mainly influenced by the methane concentration in surface sediments. However, the bacterial populations of the riverine and polar waters showed similar methane oxidation rates (0.419 and 0.400 nmol L−1 d−1), despite a higher relative abundance of methanotrophs and a higher estimated diversity in the riverine water than in the polar water. The methane turnover times ranged from 167 days in mixed water and 91 days in riverine water to only 36 days in polar water. The environmental parameters influencing the methane oxidation rate and the methanotrophic population also differed between the water masses. We postulate the presence of a riverine methanotrophic population that is limited by sub-optimal temperatures and substrate concentrations and a polar methanotrophic population that is well adapted to the cold and methane-poor polar environment but limited by a lack of nitrogen. The diffusive methane flux into the atmosphere ranged from 4 to 163 µmol m2 d−1 (median 24). The diffusive methane flux accounted for a loss of 8 % of the total methane inventory of the investigated area, whereas the methanotrophic bacteria consumed only 1 % of this methane inventory. Our results underscore the importance of measuring the methane oxidation activities in polar estuaries, and they indicate a population-level differentiation between riverine and polar water methanotrophs.
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- 2017
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19. “FEMENINA, SÍ. FEMINISTA, NO.” DEMONIZACIÓN DEL FEMINISMO EN BLOGS PORTUGUESES
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WICHELS, Susana, primary
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- 2019
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20. The Plastisphere - Uncovering tightly attached plastic 'specific' microorganisms.
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Inga Vanessa Kirstein, Antje Wichels, Elisabeth Gullans, Georg Krohne, and Gunnar Gerdts
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Medicine ,Science - Abstract
In order to understand the degradation potential of plastics in the marine environment, microorganisms that preferentially colonize and interact with plastic surfaces, as opposed to generalists potentially colonising everything, need to be identified. Accordingly, it was hypothesized that i.) plastic "specific" microorganisms are closely attached to the polymeric surface and ii.) that specificity of plastics biofilms are rather related to members of the rare biosphere. To answer these hypotheses, a three phased experiment to stepwise uncover closely attached microbes was conducted. In Phase 1, nine chemically distinct plastic films and glass were incubated in situ for 21 months in a seawater flow through system. In Phase 2, a high-pressure water jet treatment technique was used to remove the upper biofilm layers to further, in Phase 3, enrich a plastic "specific" community. To proof whether microbes colonizing different plastics are distinct from each other and from other inert hard substrates, the bacterial communities of these different substrates were analysed using 16S rRNA gene tag sequencing. Our findings indicate that tightly attached microorganisms account to the rare biosphere and suggest the presence of plastic "specific" microorganisms/assemblages which could benefit from the given plastic properties or at least grow under limited carbon resources.
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- 2019
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21. Dangerous hitchhikers? Evidence for potentially pathogenic Vibrio spp. on microplastic particles
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Kirstein, Inga V., Kirmizi, Sidika, Wichels, Antje, Garin-Fernandez, Ale, Erler, Rene, Löder, Martin, and Gerdts, Gunnar
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- 2016
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22. Neue Ideen zur Optimierung nicht klinischer Teilfunktionen
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Salfeld, Rainer, Hehner, Steffen, Wichels, Reinhard, Salfeld, Rainer, Hehner, Steffen, and Wichels, Reinhard
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- 2009
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23. Wege zur transsektoralen Versorgung
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Salfeld, Rainer, Hehner, Steffen, Wichels, Reinhard, Salfeld, Rainer, Hehner, Steffen, and Wichels, Reinhard
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- 2009
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24. Anpassung der Angebotsstrukturen
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Salfeld, Rainer, Hehner, Steffen, Wichels, Reinhard, Salfeld, Rainer, Hehner, Steffen, and Wichels, Reinhard
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- 2009
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25. Der Weg zur erfolgreichen Vermarktung von Krankenhausleistungen
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Salfeld, Rainer, Hehner, Steffen, Wichels, Reinhard, Salfeld, Rainer, Hehner, Steffen, and Wichels, Reinhard
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- 2009
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26. Qualität im Krankenhaus – Das Wohl des Patienten als wirtschaftlicher Erfolgsfaktor
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Salfeld, Rainer, Hehner, Steffen, Wichels, Reinhard, Salfeld, Rainer, Hehner, Steffen, and Wichels, Reinhard
- Published
- 2009
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27. Patientenzentrierte Behandlungsabläufe – Schlüssel zu mehr Wirtschaftlichkeit und Qualität
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Salfeld, Rainer, Hehner, Steffen, Wichels, Reinhard, Salfeld, Rainer, Hehner, Steffen, and Wichels, Reinhard
- Published
- 2009
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28. Von der Verwaltung zum Management von Krankenhäusern
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Salfeld, Rainer, Hehner, Steffen, Wichels, Reinhard, Salfeld, Rainer, Hehner, Steffen, and Wichels, Reinhard
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- 2009
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29. Die deutschen Krankenhäuser – international auf dem Weg an die Spitze
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Salfeld, Rainer, Hehner, Steffen, Wichels, Reinhard, Salfeld, Rainer, Hehner, Steffen, and Wichels, Reinhard
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- 2009
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30. Herausforderungen und Handlungsfelder für die Krankenhausführung
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Salfeld, Rainer, Hehner, Steffen, Wichels, Reinhard, Salfeld, Rainer, Hehner, Steffen, and Wichels, Reinhard
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- 2009
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31. Shell Disease Syndrome Is Associated with Reduced and Shifted Epibacterial Diversity on the Carapace of the Crustacean Cancer pagurus
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Bergen, Nils, primary, Krämer, Philipp, additional, Romberg, Julia, additional, Wichels, Antje, additional, Gerlach, Gabriele, additional, and Brinkhoff, Thorsten, additional
- Published
- 2022
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32. The travelling particles: community dynamics of biofilms on microplastics transferred along a salinity gradient
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Jessica Song, Lukas Beule, Elanor Jongmans-Hochschulz, Antje Wichels, and Gunnar Gerdts
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Biofilms ,General Medicine - Abstract
Microplastics (MP), as novel substrata for microbial colonization within aquatic ecosystems, are a matter of growing concern due to their potential to propagate foreign or invasive species across different environments. MP are known to harbour a diversity of microorganisms, yet little is understood of the dynamics of their biofilms and their capacity to successfully displace these microorganisms across different aquatic ecosystems typically marked by steep salinity gradients. To address this, we performed an in situ sequential incubation experiment to simulate MP transport from riverine to coastal seawaters using synthetic (high-density polyethylene, HDPE and tyre wear, TW) and natural (Wood) substrata. Bacterial communities on incubated particles were compared to each other as well as to those in surrounding waters, and their dynamics along the gradient investigated. All communities differed significantly from each other in their overall structure along the salinity gradient and were shaped by different ecological processes. While HDPE communities were governed by environmental selection, those on TW and Wood were dominated by stochastic events of dispersal and drift. Upon transfer into coastal seawaters, an almost complete turnover was observed among HDPE and TW communities. While synthetic particles displaced a minor proportion of communities across the salinity gradient, some of these comprised putatively pathogenic and resistant taxa. Our findings present an extensive assessment of MP biofilms and their dynamics upon displacement across different aquatic systems, presenting new insights into the role of MP as transport vectors. Also see: https://micro2022.sciencesconf.org/426985/document, In MICRO 2022, Online Atlas Edition: Plastic Pollution from MACRO to nano
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- 2022
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33. Einleitung
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Wichels, Daniel and Wichels, Daniel
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- 2002
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34. Schlussbetrachtung
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Wichels, Daniel and Wichels, Daniel
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- 2002
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35. Handlungsempfehlungen zur Gestaltung der Kapitalmarktkommunikation mit Finanzanalysten
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Wichels, Daniel and Wichels, Daniel
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- 2002
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36. Bedeutung von Finanzanalysten auf den Kapitalmärkten und Implikationen für Investor Relations
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Wichels, Daniel and Wichels, Daniel
- Published
- 2002
- Full Text
- View/download PDF
37. Empirische Untersuchung zum Informationsbedarf von Finanzanalysten in der Automobilindustrie
- Author
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Wichels, Daniel and Wichels, Daniel
- Published
- 2002
- Full Text
- View/download PDF
38. Grundlagen
- Author
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Wichels, Daniel and Wichels, Daniel
- Published
- 2002
- Full Text
- View/download PDF
39. Diversity and activity of marine bacterioplankton during a diatom bloom in the North Sea assessed by total RNA and pyrotag sequencing
- Author
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Klindworth, Anna, Mann, Alexander J., Huang, Sixing, Wichels, Antje, Quast, Christian, Waldmann, Jost, Teeling, Hanno, and Glöckner, Frank Oliver
- Published
- 2014
- Full Text
- View/download PDF
40. Highly diverse flavobacterial phages isolated from North Sea spring blooms
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Nina Bartlau, Rudolf Amann, Evelien M. Adriaenssens, Bernhard M. Fuchs, Antje Wichels, Cristina Moraru, Georg Krohne, and Anneke Heins
- Subjects
Water microbiology ,Host (biology) ,fungi ,Zoology ,Eutrophication ,Biology ,Spring bloom ,biology.organism_classification ,Microbiology ,Article ,Habitat ,Microbial population biology ,Lytic cycle ,Phytoplankton ,Humans ,Metagenome ,CRISPR ,Bacteriophages ,North Sea ,Flavobacteriaceae ,Ecology, Evolution, Behavior and Systematics ,Flavobacteriia - Abstract
It is generally recognized that phages are a mortality factor for their bacterial hosts. This could be particularly true in spring phytoplankton blooms, which are known to be closely followed by a highly specialized bacterial community. We hypothesized that phages modulate these dense heterotrophic bacteria successions following phytoplankton blooms. In this study, we focused on Flavobacteriia, because they are main responders during these blooms and have an important role in the degradation of polysaccharides. A cultivation-based approach was used, obtaining 44 lytic flavobacterial phages (flavophages), representing twelve new species from two viral realms. Taxonomic analysis allowed us to delineate ten new phage genera and ten new families, from which nine and four, respectively, had no previously cultivated representatives. Genomic analysis predicted various life styles and genomic replication strategies. A likely eukaryote-associated host habitat was reflected in the gene content of some of the flavophages. Detection in cellular metagenomes and by direct-plating showed that part of these phages were actively replicating in the environment during the 2018 spring bloom. Furthermore, CRISPR/Cas spacers and re-isolation during two consecutive years suggested that, at least part of the new flavophages are stable components of the microbial community in the North Sea. Together, our results indicate that these diverse flavophages have the potential to modulate their respective host populations.
- Published
- 2021
41. List of Contributors
- Author
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Abbas, B., primary, Abreu, A., additional, Adams, R., additional, Adolfsson-Erici, M., additional, Afonso, A., additional, Afonso-Olivares, C., additional, Agirbas, E., additional, Aguiló, J.M., additional, Airoldi, L., additional, Aksoy, H., additional, Albentosa, M., additional, Alcaro, L., additional, Aliani, S., additional, Al-Maslamani, I., additional, Alomar, C., additional, Altin, D., additional, Álvarez, E., additional, Amaral-Zettler, L.A., additional, Amato, E., additional, Anderson, A., additional, Andrady, A.L., additional, Andrius, G., additional, Angel, D., additional, Ariese, F., additional, Arp, H.P., additional, Asensio, M., additional, Assidqi, K., additional, Avio, C.G., additional, Aytan, U., additional, Bahri, T., additional, Baini, M., additional, Bakir, A., additional, Ball, H., additional, Baranyi, C., additional, Barboza, L.G.A., additional, Barg, U., additional, Bargelloni, L., additional, Barras, H., additional, Barrera, C., additional, Barria, P., additional, Barrows, A., additional, Barth, A., additional, Batel, A., additional, Baztan, J., additional, Baztan, P., additional, Beiras, R., additional, Benedetti, M., additional, Berber, A.A., additional, Berber, N., additional, Bergmann, M., additional, Berlino, M., additional, Berrow, S., additional, Bessa, F., additional, Besseling, E., additional, Beyer, B., additional, Binaglia, M., additional, Bizjak, T., additional, Bjorndal, K.A., additional, Blust, R., additional, Boertien, M., additional, Bolten, A.B., additional, Booth, A.M., additional, Bounoua, B., additional, Bourseau, P., additional, Brahimi, N., additional, Bramini, M., additional, Brennholt, N., additional, Breuninger, E., additional, Bried, J., additional, Broderick, A., additional, Broglio, E., additional, Browne, M.A., additional, Bruzaud, S., additional, Buceta, J., additional, Buchinger, S., additional, Budimir, S., additional, Budzin-ski, H., additional, Butter, E., additional, Cachot, J., additional, Caetano, M., additional, Callaghan, A., additional, Camedda, A., additional, Capella, S., additional, Cardelli, L., additional, Carpentieri, S., additional, Carrasco, A., additional, Carriço, R., additional, Caruso, A., additional, Cassone, A.-L., additional, Castillo, A., additional, Castro, R.O., additional, Catarino, A.I., additional, Cazenave, P.W., additional, Çelik, İ., additional, Cerralbo, P., additional, César, G., additional, Chouinard, O., additional, Chubarenko, I., additional, Chubarenko, I.P., additional, Cicero, A.M., additional, Clarindo, G., additional, Clarke, B., additional, Clérandeau, C., additional, Clüsener-Godt, M., additional, Codina-García, M., additional, Cole, M., additional, Collard, F., additional, Collignon, A., additional, Collins, T., additional, Compa, M., additional, Conan, P., additional, Constant, M., additional, Cordier, M., additional, Courtene-Jones, W., additional, Cousin, X., additional, Covelo, P., additional, Cózar, A., additional, Crichton, E., additional, Crispi, O., additional, Cronin, M., additional, Croot, P.L., additional, Cruz, M.J., additional, d’Errico, G., additional, Dâmaso, C., additional, Das, K., additional, de Alencastro, L.F., additional, de Araujo, F.V., additional, de Boer, J.F., additional, de Lucia, G.A., additional, Debeljak, P., additional, Dehaut, A., additional, Deudero, S., additional, Devrieses, L., additional, Di Vito, S., additional, Díaz, A., additional, Donohue, J., additional, Doumenq, P., additional, Doyle, T.K., additional, Dris, R., additional, Druon, J.-N., additional, Duarte, C.M., additional, Duflos, G., additional, Dumontier, M., additional, Duncan, E., additional, Dussud, C., additional, Eckerlebe, A., additional, Egelkraut-Holtus, M., additional, Eidsvoll, D.P., additional, Ek, C., additional, Elena, S., additional, Elineau, A., additional, Enevoldsen, H., additional, Eppe, G., additional, Eriksen, M., additional, Ernsteins, R., additional, Espino, M., additional, Estévez-Calvar, N., additional, Ewins, C., additional, Fabre, P., additional, Faimali, M., additional, Fattorini, D., additional, Faure, F., additional, Ferrando, S., additional, Ferreira, J.C., additional, Ferreira-da-Costa, M., additional, Fileman, E., additional, Fischer, M., additional, Fortunato, A.B., additional, Fossi, M.C., additional, Foulon, V., additional, Frank, A., additional, Frenzel, M., additional, Frère, L., additional, Frias, J.P.G.L., additional, Frick, H., additional, Froneman, P.W., additional, Gabet, V.M., additional, Gabrielsen, G.W., additional, Gago, J., additional, Gajst, T., additional, Galgani, F., additional, Gallinari, M., additional, Galloway, T.S., additional, Gamarro, E.G., additional, Gambardella, C., additional, Garaventa, F., additional, Garcia, S., additional, Garrabou, J., additional, Garrido, P., additional, Gary, S.F., additional, Gasperi, J., additional, Gaze, W., additional, Geertz, T., additional, Gelado-Caballero, M.D., additional, George, M., additional, Gercken, J., additional, Gerdts, G., additional, Ghiglione, J.-F., additional, Gies, E., additional, Gilbert, B., additional, Giménez, L., additional, Glassom, D., additional, Glockzin, M., additional, Godley, B., additional, Goede, K., additional, Goksøyr, A., additional, Gómez, M., additional, Gómez-Parra, A., additional, González-Marco, D., additional, González-Solís, J., additional, Gorbi, S., additional, Gorokhova, E., additional, Gorsky, G., additional, Gosch, M., additional, Grose, J., additional, Guebitz, G.M., additional, Guedes-Alonso, R., additional, Guijarro, B., additional, Guilhermino, L., additional, Gundry, T., additional, Gutow, L., additional, Haave, M., additional, Haeckel, M., additional, Haernvall, K., additional, Hajbane, S., additional, Hamann, M., additional, Hämer, J., additional, Hamm, T., additional, Hansen, B.H., additional, Hardesty, B.D., additional, Harth, B., additional, Hartikainen, S., additional, Hassellöv, M., additional, Hatzky, S., additional, Healy, M.G., additional, Hégaret, H., additional, Henry, T.B., additional, Hermabessiere, L., additional, Hernández-Brito, J.J., additional, Hernandez-Gonzalez, A., additional, Hernandez-Milian, G., additional, Hernd, G., additional, Herrera, A., additional, Herring, C., additional, Herzke, D., additional, Heussner, S., additional, Hidalgo-Ruz, V., additional, Himber, C., additional, Holland, M., additional, Hong, N.-H., additional, Horton, A.A., additional, Horvat, P., additional, Huck, T., additional, Huhn, M., additional, Huvet, A., additional, Iglesias, M., additional, Igor, C., additional, Isachenko, I.A., additional, Ivar do Sul, J-A., additional, Jahnke, A., additional, Janis, B., additional, Janis, K., additional, Janis, U., additional, Jemec, A., additional, Jiménez, J.C., additional, Johnsen, H., additional, Jorgensen, B., additional, Jørgensen, J.H., additional, Jörundsdóttir, H., additional, Jung, Y.-J., additional, Kedzierski, M., additional, Keiter, S., additional, Kershaw, P., additional, Kerhervé, P., additional, Kesy, K., additional, Khan, F., additional, Khatmullina, L.I., additional, Kirby, J., additional, Kiriakoulakis, K., additional, Klein, R., additional, Klunderud, T., additional, Knudsen, C.M.H., additional, Knudsen, T.B., additional, Kochleus, C., additional, Koelmans, A.A., additional, Kögel, T., additional, Koistinen, A., additional, Kopke, K., additional, Korez, Š., additional, Kowalski, N., additional, Kreikemeyer, B., additional, Kroon, F., additional, Krumpen, T., additional, Krzan, A., additional, Kržan, A., additional, Labrenz, M., additional, Lacroix, C., additional, Ladirat, L., additional, Laforsch, C., additional, Lagarde, F., additional, Lahive, E., additional, Lambert, C., additional, Lapucci, C., additional, Lattin, G., additional, Law, K.L., additional, Le Roux, F., additional, Le Souef, K., additional, Le Tilly, V., additional, Lebreton, L., additional, Leemans, E., additional, Lehtiniemi, M., additional, Lenz, M., additional, Leskinen, J., additional, Leslie, H., additional, Leslie, H.A., additional, Levasseur, C., additional, Lewis, C., additional, Licandro, P., additional, Lind, K., additional, Lindeque, P., additional, Lindeque, P.K., additional, Lips, I., additional, Liria, A., additional, Liria-Loza, A., additional, Llinás, O., additional, Loiselle, S.A., additional, Long, M., additional, Lorenz, C., additional, Lorenzo, S.M., additional, Loubar, K., additional, Luna-Jorquera, G., additional, Lusher, A.L., additional, Macchia, V., additional, MacGabban, S., additional, Mackay, K., additional, MacLeod, M., additional, Maes, T., additional, Magaletti, E., additional, Maggiore, A., additional, Magnusson, K., additional, Mahon, A.M., additional, Makorič, P., additional, Mallow, O., additional, Marques, J., additional, Marsili, L., additional, Martí, E., additional, Martignac, M., additional, Martin, J., additional, Martínez, I., additional, Martínez, J., additional, Martinez-Gil, M., additional, Martins, H.R., additional, Matiddi, M., additional, Maximenko, N., additional, Mazlum, R., additional, Mcadam, R., additional, Mcknight, L., additional, McNeal, A.W., additional, Measures, J., additional, Mederos, M.S., additional, Mendoza, J., additional, Meyer, M.S., additional, Miguelez, A., additional, Milan, M., additional, Militão, T., additional, Miller, R.Z., additional, Mino-Vercellio-Verollet, M., additional, Mir, G., additional, Miranda-Urbina, D., additional, Misurale, F., additional, Montesdeoca-Esponda, S., additional, Mora, J., additional, Morgana, S., additional, Moriceau, B., additional, Morin, B., additional, Morley, A., additional, Morrison, L., additional, Murphy, F., additional, Naidoo, T., additional, Näkki, P., additional, Napper, I.E., additional, Narayanaswamy, B.E., additional, Nash, R., additional, Negri, A., additional, Nel, H.A., additional, Nerheim, M.S., additional, Nerland, I.L., additional, Neto, J., additional, Neves, V., additional, Nies, H., additional, Noel, M., additional, Nor, N.H.M., additional, Noren, F., additional, O’ Connell, B., additional, O’ Connor, I., additional, Obbard, J.P., additional, Oberbeckmann, S., additional, Obispo, R., additional, Officer, R., additional, Ogonowski, M., additional, Orbea, A., additional, Ortlieb, M., additional, Osborn, A.M., additional, Ostiategui-Francia, P., additional, Packard, T., additional, Pahl, S., additional, Palatinus, A., additional, Palmqvist, A., additional, Pannetier, P., additional, Panti, C., additional, Parmentier, E., additional, Pasanen, P., additional, Patarnello, T., additional, Pattiaratchi, C., additional, Pauletto, M., additional, Paulus, M., additional, Pavlekovsky, K., additional, Pedersen, H.B., additional, Pedrotti, M.-L., additional, Peeken, I., additional, Peeters, D., additional, Peeters, E., additional, Pellegrini, D., additional, Perales, J.A., additional, Perez, E., additional, Perz, V., additional, Petit, S., additional, Pflieger, M., additional, Pham, C.K., additional, Piazza, V., additional, Pinto, M., additional, Planells, O., additional, Plaza, M., additional, Pompini, O., additional, Potthoff, A., additional, Prades, L., additional, Primpke, S., additional, Proietti, M., additional, Proskurowski, G., additional, Puig, C., additional, Pujo-Pay, M., additional, Pullerits, K., additional, Queirós, A.M., additional, Quinn, B., additional, Raimonds, E., additional, Ramis-Pujol, J., additional, Rascher-Friesenhausen, R., additional, Reardon, E., additional, Regoli, F., additional, Reichardt, A.M., additional, Reifferscheid, G., additional, Reilly, K., additional, Reisser, J., additional, Riba, I., additional, Ribitsch, D., additional, Rinnert, E., additional, Rios, N., additional, Rist, S.E., additional, Rivadeneira, M.M., additional, Rivière, G., additional, Robbens, J., additional, Robertson, C.J.R., additional, Rocher, V., additional, Rochman, C.M., additional, Rodrigues, M., additional, Rodriguez, Y., additional, Rodríguez, A., additional, Rodríguez, G., additional, Rodríguez, J.R.B., additional, Rodríguez, S., additional, Rodríguez, Y., additional, Rogan, E., additional, Rojo-Nieto, E., additional, Romeo, T., additional, Ross, P.S., additional, Roveta, A., additional, Rowland, S.J., additional, Ruckstuhl, N.A., additional, Ruiz-Fernández, A-C., additional, Ruiz-Orejón, L.F., additional, Runge, J., additional, Russell, M., additional, Saavedra, C., additional, Saborowski, R., additional, Sahin, B.E., additional, Sailley, S., additional, Sakaguchi-Söder, K., additional, Salaverria, I., additional, Sánchez-Arcilla, A., additional, Sánchez-Nieva, J., additional, Sanderson, W., additional, Santana-Rodríguez, J.J., additional, Santana-Viera, S., additional, Santos, M.B., additional, Santos, M.R., additional, Sanz, M.R., additional, Sardá, R., additional, Savelli, H., additional, Schoeneich-Argent, R., additional, Scholz-Böttcher, B.M., additional, Sciacca, F., additional, Scofield, R.P., additional, Setälä, O., additional, Selenius, M., additional, Sempere, R., additional, Senturk, Y., additional, Shashoua, Y., additional, Sherman, P., additional, Sick, C., additional, Siegel, D., additional, Sierra, J.P., additional, Silva, F., additional, Silvestri, C., additional, Sintija, G., additional, Sire, O., additional, Slat, B., additional, Smit, A., additional, Sobral, P., additional, Sorvari, J., additional, Sosa-Ferrera, Z., additional, Sotillo, M.G., additional, Soudant, P., additional, Speidel, L., additional, Spurgeon, D.J., additional, Steer, M.K., additional, Steindal, C.C., additional, Stifanese, R., additional, Štindlová, A., additional, Stuurman, L., additional, Suaria, G., additional, Suazo, C.G., additional, Sureda, A., additional, Surette, C., additional, Svendsen, C., additional, Syberg, K., additional, Tairova, Z., additional, Talvitie, J., additional, Tassin, B., additional, Tazerout, M., additional, Tekman, M.B., additional, ter Halle, A., additional, Thiel, M., additional, Thomas, K.V., additional, Thompson, R.C., additional, Tinkara, T., additional, Tirelli, V., additional, Tomassetti, P., additional, Toorman, E., additional, Toppe, J., additional, Tornambè, A., additional, Torres, R., additional, Torres-Padrón, M.E., additional, Underwood, A.J., additional, Urbina, M., additional, Usategui-Martín, A., additional, Usta, R., additional, Valdés, L., additional, Valente, A., additional, Valentina, T., additional, van Arkel, K., additional, Van Colen, C., additional, Van Der Hal, N., additional, van Franeker, J.A., additional, Van Herwerden, L., additional, Van Loosdrecht, M., additional, van Oyen, A., additional, Vandeperre, F., additional, Vanderlinden, J-P., additional, Vani, D., additional, Vasconcelos, L., additional, Vega-Moreno, D., additional, Ventero, A., additional, Vethaak, A.D., additional, Vianello, A., additional, Vicioso, M., additional, Vieira, L.R., additional, Viršek, M.K., additional, Vos, M., additional, Wahl, M., additional, Wallace, N., additional, Walton, A., additional, Waniek, J.J., additional, Watts, A., additional, Webster, L., additional, Wesch, C., additional, Whitfield, E., additional, Wichels, A., additional, Wieczorek, A.M., additional, Wilcox, C., additional, Williams, R.J., additional, Wong-Wah-Chung, P., additional, Wright, S., additional, Wyles, K.J., additional, Young, R., additional, Yurtsever, M., additional, Yurtsever, U., additional, Zada, L., additional, Zamani, N.P., additional, and Zampetti, G., additional
- Published
- 2017
- Full Text
- View/download PDF
42. Linking Education and Science to Increase Awareness of Marine Plastic Litter—Distribution of Plastic Waste on Beaches of the German Bight
- Author
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Wichels, A., primary, Harth, B., additional, and Gerdts, G., additional
- Published
- 2017
- Full Text
- View/download PDF
43. Comparative Omics and Trait Analyses of Marine Pseudoalteromonas Phages Advance the Phage OTU Concept
- Author
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Melissa B. Duhaime, Natalie Solonenko, Simon Roux, Nathan C. Verberkmoes, Antje Wichels, and Matthew B. Sullivan
- Subjects
phage ,marine microbiology ,particle-associated ,comparative genomics ,Pseudoalteromonas ,Microbiology ,QR1-502 - Abstract
Viruses influence the ecology and evolutionary trajectory of microbial communities. Yet our understanding of their roles in ecosystems is limited by the paucity of model systems available for hypothesis generation and testing. Further, virology is limited by the lack of a broadly accepted conceptual framework to classify viral diversity into evolutionary and ecologically cohesive units. Here, we introduce genomes, structural proteomes, and quantitative host range data for eight Pseudoalteromonas phages isolated from Helgoland (North Sea, Germany) and use these data to advance a genome-based viral operational taxonomic unit (OTU) definition. These viruses represent five new genera and inform 498 unaffiliated or unannotated protein clusters (PCs) from global virus metagenomes. In a comparison of previously sequenced Pseudoalteromonas phage isolates (n = 7) and predicted prophages (n = 31), the eight phages are unique. They share a genus with only one other isolate, Pseudoalteromonas podophage RIO-1 (East Sea, South Korea) and two Pseudoalteromonas prophages. Mass-spectrometry of purified viral particles identified 12–20 structural proteins per phage. When combined with 3-D structural predictions, these data led to the functional characterization of five previously unidentified major capsid proteins. Protein functional predictions revealed mechanisms for hijacking host metabolism and resources. Further, they uncovered a hybrid sipho-myovirus that encodes genes for Mu-like infection rarely described in ocean systems. Finally, we used these data to evaluate a recently introduced definition for virus populations that requires members of the same population to have >95% average nucleotide identity across at least 80% of their genes. Using physiological traits and genomics, we proposed a conceptual model for a viral OTU definition that captures evolutionarily cohesive and ecologically distinct units. In this trait-based framework, sensitive hosts are considered viral niches, while host ranges and infection efficiencies are tracked as viral traits. Quantitative host range assays revealed conserved traits within virus OTUs that break down between OTUs, suggesting the defined units capture niche and fitness differentiation. Together these analyses provide a foundation for model system-based hypothesis testing that will improve our understanding of marine copiotrophs, as well as phage–host interactions on the ocean particles and aggregates where Pseudoalteromonas thrive.
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- 2017
- Full Text
- View/download PDF
44. Nuevos desafíos en Relaciones Públicas 2.0: La creciente influencia de las plataformas de online review en Turismo/New Challenges in Public Relations 2.0: The growing influence of online review platforms in Tourism
- Author
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Susana Wichels
- Subjects
comunicación turística ,comunicación 2.0 ,relaciones públicas 2.0 ,redes sociales ,TripAdvisor. ,tourism communication ,communication 2.0 ,public relations 2.0 ,social media ,TripAdvisor ,Political science - Abstract
La influencia de las Tecnologías de la Información y Comunicación (TIC) en el sector turístico y en especial en la comunicación de los productos y en la gestión de las relaciones con los públicos es ampliamente conocida. Las aplicaciones Web 2.0 basadas en comentarios y valoraciones de usuarios a escala mundial, en concreto TripAdvisor, influencian las prácticas en relaciones públicas y comunicación turísticas obligando a una cuidada gestión de la reputación online. Si por un lado las nuevas herramientas 2.0 están à disposición de las organizaciones para comunicar con sus públicos, por otro lado son también fuente de comentários y valoraciones directas que pueden reforzar positivamente la imagen de una organización o contribuir con opiniones negativas que desafian nuevas práticas en relaciones públicas. A fin de determinar si estas aplicaciones están afectando el comportamiento de planificación y reservas de productos y servicios turísticos, hicimos un estudio basado en el análisis de Burguess y Kerr (2012) dirigida a turistas portugueses. / The influence of Information Technology and Communication (ICT) in the tourism sector, especially in communicating products and managing relations with the public is recognized widely. Web 2.0 applications based on reviews and ratings from users worldwide, specifically TripAdvisor, are influencing practices in public relations and communication in tourism and obliging a careful management of online reputation. If on one hand, the new 2.0 tools allow a direct communication with the audiences, on the other hand they also provide comments and reviews that can positively reinforce or undermine the image of an organization, challenging new practices in public relations. To determine how internet, and in particular online review applications are affecting the behavior of researching and booking, we have prepared a study based on Burguess & Kerr (2012) analysis, about traveler’s use of the internet: understanding Portuguese planning and booking behavior.
- Published
- 2014
- Full Text
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45. Diatom fucan polysaccharide precipitates carbon during algal blooms
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Andreas Sichert, Rudolf Amann, Bernhard M. Fuchs, Jutta Niggemann, T. Ben Francis, Hanno Teeling, Antje Wichels, Thomas Schweder, Daniel Bartosik, William G.T. Willats, Dörte Becher, Jan-Hendrik Hehemann, and Silvia Vidal-Melgosa
- Subjects
0301 basic medicine ,Carbon Sequestration ,Science ,030106 microbiology ,Glycobiology ,General Physics and Astronomy ,chemistry.chemical_element ,Carbon sequestration ,Polysaccharide ,Algal bloom ,Antibodies ,Article ,General Biochemistry, Genetics and Molecular Biology ,Carbon cycle ,Epitopes ,03 medical and health sciences ,Polysaccharides ,Seawater ,North sea ,Glycomics ,Diatoms ,chemistry.chemical_classification ,Multidisciplinary ,biology ,Chemistry ,fungi ,Chaetoceros ,General Chemistry ,Eutrophication ,biology.organism_classification ,Carbon ,030104 developmental biology ,Diatom ,Marine chemistry ,Environmental chemistry ,North Sea - Abstract
The formation of sinking particles in the ocean, which promote carbon sequestration into deeper water and sediments, involves algal polysaccharides acting as an adhesive, binding together molecules, cells and minerals. These as yet unidentified adhesive polysaccharides must resist degradation by bacterial enzymes or else they dissolve and particles disassemble before exporting carbon. Here, using monoclonal antibodies as analytical tools, we trace the abundance of 27 polysaccharide epitopes in dissolved and particulate organic matter during a series of diatom blooms in the North Sea, and discover a fucose-containing sulphated polysaccharide (FCSP) that resists enzymatic degradation, accumulates and aggregates. Previously only known as a macroalgal polysaccharide, we find FCSP to be secreted by several globally abundant diatom species including the genera Chaetoceros and Thalassiosira. These findings provide evidence for a novel polysaccharide candidate to contribute to carbon sequestration in the ocean., The fate of ocean carbon is determined by the balance between primary productivity and heterotrophic breakdown of that photosynthate. Here the authors show that diatoms produce a polysaccharide that resists bacterial degradation, accumulates, aggregates and stores carbon during spring blooms.
- Published
- 2021
46. Management von Kapitalmarkterwartungen
- Author
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Achleitner, Paul, Wichels, Daniel, Ebel, Bernhard, editor, and Hofer, Markus B., editor
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- 2003
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47. G
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Brixner, Ulrich, Achleitner, Paul, Wichels, Daniel, Krumnow, Jürgen, editor, Gramlich, Ludwig, editor, Lange, Thomas A., editor, and Dewner, Thomas M., editor
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- 2002
- Full Text
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48. Effect of elevated CO2 on the dynamics of particle-attached and free-living bacterioplankton communities in an Arctic fjord
- Author
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S. Romac, U. Riebesell, J. I. Nissimov, L. Bittner, J. Gilbert, J. La Roche, A.-S. Roy, H. Schunck, A. Wichels, G. Gerdts, J. Piontek, M. Sperling, and A. Engel
- Subjects
Ecology ,QH540-549.5 ,Life ,QH501-531 ,Geology ,QE1-996.5 - Abstract
In the frame of the European Project on Ocean Acidification (EPOCA), the response of an Arctic pelagic community (pCO2 was investigated. For this purpose 9 large-scale in situ mesocosms were deployed in Kongsfjorden, Svalbard (78°56.2´ N, 11°53.6´ E), in 2010. The present study investigates effects on the communities of particle-attached (PA; >3 μm) and free-living (FL; < 3 μm > 0.2 μm) bacteria by Automated Ribosomal Intergenic Spacer Analysis (ARISA) in 6 of the mesocosms, ranging from 185 to 1050 μatm initial pCO2, and the surrounding fjord. ARISA was able to resolve, on average, 27 bacterial band classes per sample and allowed for a detailed investigation of the explicit richness and diversity. Both, the PA and the FL bacterioplankton community exhibited a strong temporal development, which was driven mainly by temperature and phytoplankton development. In response to the breakdown of a picophytoplankton bloom, numbers of ARISA band classes in the PA community were reduced at low and medium CO2 (~ 185–685 μatm) by about 25%, while they were more or less stable at high CO2 (~ 820–1050 μatm). We hypothesise that enhanced viral lysis and enhanced availability of organic substrates at high CO2 resulted in a more diverse PA bacterial community in the post-bloom phase. Despite lower cell numbers and extracellular enzyme activities in the post-bloom phase, bacterial protein production was enhanced in high CO2 mesocosms, suggesting a positive effect of community richness on this function and on carbon cycling by bacteria.
- Published
- 2013
- Full Text
- View/download PDF
49. Substrate-Controlled Succession of Marine Bacterioplankton Populations Induced by a Phytoplankton Bloom
- Author
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Teeling, Hanno, Fuchs, Bernhard M., Becher, Dörte, Klockow, Christine, Gardebrecht, Antje, Bennke, Christin M., Kassabgy, Mariette, Huang, Sixing, Mann, Alexander ]., Waldmann, Jost, Weber, Marc, Klindworth, Anna, Otto, Andreas, Lange, Jana, Bernhardt, Jörg, Reinsch, Christine, Hecker, Michael, Peplies, Jörg, Bockelmann, Frank D., Callies, Ulrich, Gerdts, Gunnar, Wichels, Antje, Wiltshire, Karen H., Glöckner, Frank Oliver, Schweder, Thomas, and Amann, Rudolf
- Published
- 2012
- Full Text
- View/download PDF
50. Recurring patterns in bacterioplankton dynamics during coastal spring algae blooms
- Author
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Hanno Teeling, Bernhard M Fuchs, Christin M Bennke, Karen Krüger, Meghan Chafee, Lennart Kappelmann, Greta Reintjes, Jost Waldmann, Christian Quast, Frank Oliver Glöckner, Judith Lucas, Antje Wichels, Gunnar Gerdts, Karen H Wiltshire, and Rudolf I Amann
- Subjects
marine bacterioplankton ,marine phytoplankton ,marine microbial biodiversity ,bacterial decomposition ,algal biomass ,blooms ,Medicine ,Science ,Biology (General) ,QH301-705.5 - Abstract
A process of global importance in carbon cycling is the remineralization of algae biomass by heterotrophic bacteria, most notably during massive marine algae blooms. Such blooms can trigger secondary blooms of planktonic bacteria that consist of swift successions of distinct bacterial clades, most prominently members of the Flavobacteriia, Gammaproteobacteria and the alphaproteobacterial Roseobacter clade. We investigated such successions during spring phytoplankton blooms in the southern North Sea (German Bight) for four consecutive years. Dense sampling and high-resolution taxonomic analyses allowed the detection of recurring patterns down to the genus level. Metagenome analyses also revealed recurrent patterns at the functional level, in particular with respect to algal polysaccharide degradation genes. We, therefore, hypothesize that even though there is substantial inter-annual variation between spring phytoplankton blooms, the accompanying succession of bacterial clades is largely governed by deterministic principles such as substrate-induced forcing.
- Published
- 2016
- Full Text
- View/download PDF
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