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Dietary fibers (DF) are important components of human and animal diets. However, they can decrease protein digestibility and absorption and thus the nutritional value of a food. The aim of this study was to investigate how the form of delivery of pea DF impacted the integrity of the intestinal barrier and, thereby, the potential absorption of molecules. To this end, two pea flours, with either intact or ruptured cell walls, and two controls, pea fibers and pea protein, were digested in vitro and the digesta obtained applied onto a jejunum porcine cell line (IPEC-J2 cells). Cell viability and integrity were evaluated by transepithelial electrical resistance measurement, colorimetric assay (MTS), and immunohistochemistry for tight junction proteins. Additionally, the diffusion of FITC-dextran (FD4) and lucifer yellow (LY) through the epithelial cell monolayers was monitored. The digested pea samples did not alter the IPEC-J2 viability and permeability. For instance, no difference in the diffusion of molecules either FD4 or LY across the monolayers was observed between the different digesta and the control. Similarly, no effect was observed in ZO-1 labeling intensity compared to the control. This study demonstrated that intestinal integrity was maintained whether pea cell walls were intact or ruptured.

Milk stasis in the mammary gland is a situation that induces a reduction in milk yield and a change in its composition. Theses changes could be related to a decrease in the number and/or activity of secretory cells. Previous observations showed that a disruption of cell junctions is one of the early phenomena accompanying milk accumulation in the mammary gland. The aim of the present study was to investigate the local effect of 36 h milk accumulation on mammary cells activity, apoptosis and proliferation. We also studied the expression of cell junction proteins after 36 h of milk stasis. We observed a decrease in the quantity of milk produced after 36 h of milk stasis in goat. Lower milk lactose and protein yields were also observed, which was associated with a decrease in the transcripts of genes involved in synthesis of these constituents, such as alpha-lactalbumin and kappa-casein. Mammary gland apoptosis was more intensive than mammary cell proliferation after 36 h of milk accumulation. All these changes were associated with an up-regulation of E-cadherin protein and increase of its transcripts levels. It could suggest that these adjustments are made in order to limit losses of secretory cell number and activity.