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1. EUREC4A

Author

Stevens, B, Bony, S, Farrell, D, Ament, F, Blyth, A, Fairall, C, Karstensen, J, Quinn, P, Speich, S, Acquistapace, C, Aemisegger, F, Albright, A, Bellenger, H, Bodenschatz, E, Caesar, K, Chewitt-Lucas, R, De Boer, G, Delanoe, J, Denby, L, Ewald, F, Fildier, B, Forde, M, George, G, Gross, S, Hagen, M, Hausold, A, Heywood, K, Hirsch, L, Jacob, M, Jansen, F, Kinne, S, Klocke, D, Kolling, T, Konow, H, Lothon, M, Mohr, W, Naumann, A, Nuijens, L, Olivier, L, Pincus, R, Pohlker, M, Reverdin, G, Roberts, G, Schnitt, S, Schulz, H, Pier Siebesma, A, Stephan, C, Sullivan, P, Touze-Peiffer, L, Vial, J, Vogel, R, Zuidema, P, Alexander, N, Alves, L, Arixi, S, Asmath, H, Bagheri, G, Baier, K, Bailey, A, Baranowski, D, Baron, A, Barrau, S, Barrett, P, Batier, F, Behrendt, A, Bendinger, A, Beucher, F, Bigorre, S, Blades, E, Blossey, P, Bock, O, Boing, S, Bosser, P, Bourras, D, Bouruet-Aubertot, P, Bower, K, Branellec, P, Branger, H, Brennek, M, Brewer, A, Brilouet, P, Brugmann, B, Buehler, S, Burke, E, Burton, R, Calmer, R, Canonici, J, Carton, X, Cato, G, Charles, J, Chazette, P, Chen, Y, Chilinski, M, Choularton, T, Chuang, P, Clarke, S, Coe, H, Cornet, C, Coutris, P, Couvreux, F, Crewell, S, Cronin, T, Cui, Z, Cuypers, Y, Daley, A, Damerell, G, Dauhut, T, Deneke, H, Desbios, J, Dorner, S, Donner, S, Douet, V, Drushka, K, Dutsch, M, Ehrlich, A, Emanuel, K, Emmanouilidis, A, Etienne, J, Etienne-Leblanc, S, Faure, G, Feingold, G, Ferrero, L, Fix, A, Flamant, C, Flatau, P, Foltz, G, Forster, L, Furtuna, I, Gadian, A, Galewsky, J, Gallagher, M, Gallimore, P, Gaston, C, Gentemann, C, Geyskens, N, Giez, A, Gollop, J, Gouirand, I, Gourbeyre, C, De Graaf, D, De Groot, G, Grosz, R, Guttler, J, Gutleben, M, Hall, K, Harris, G, Helfer, K, Henze, D, Herbert, C, Holanda, B, Ibanez-Landeta, A, Intrieri, J, Iyer, S, Julien, F, Kalesse, H, Kazil, J, Kellman, A, Kidane, A, Kirchner, U, Klingebiel, M, Korner, M, Kremper, L, Kretzschmar, J, Kruger, O, Kumala, W, Kurz, A, L'Hegaret, P, Labaste, M, Lachlan-Cope, T, Laing, A, Landschutzer, P, Lang, T, Lange, D, Lange, I, Laplace, C, Lavik, G, Laxenaire, R, Lebihan, C, Leandro, M, Lefevre, N, Lena, M, Lenschow, D, Li, Q, Lloyd, G, Los, S, Losi, N, Lovell, O, Luneau, C, Makuch, P, Malinowski, S, Manta, G, Marinou, E, Marsden, N, Masson, S, Maury, N, Mayer, B, Mayers-Als, M, Mazel, C, Mcgeary, W, Mcwilliams, J, Mech, M, Mehlmann, M, Meroni, A, Mieslinger, T, Minikin, A, Minnett, P, Moller, G, Avalos, Y, Muller, C, Musat, I, Napoli, A, Neuberger, A, Noisel, C, Noone, D, Nordsiek, F, Nowak, J, Oswald, L, Parker, D, Peck, C, Person, R, Philippi, M, Plueddemann, A, Pohlker, C, Portge, V, Poschl, U, Pologne, L, Posyniak, M, Prange, M, Melendez, E, Radtke, J, Ramage, K, Reimann, J, Renault, L, Reus, K, Reyes, A, Ribbe, J, Ringel, M, Ritschel, M, Rocha, C, Rochetin, N, Rottenbacher, J, Rollo, C, Royer, H, Sadoulet, P, Saffin, L, Sandiford, S, Sandu, I, Schafer, M, Schemann, V, Schirmacher, I, Schlenczek, O, Schmidt, J, Schroder, M, Schwarzenboeck, A, Sealy, A, Senff, C, Serikov, I, Shohan, S, Siddle, E, Smirnov, A, Spath, F, Spooner, B, Katharina Stolla, M, Szkolka, W, De Szoeke, S, Tarot, S, Tetoni, E, Thompson, E, Thomson, J, Tomassini, L, Totems, J, Ubele, A, Villiger, L, Von Arx, J, Wagner, T, Walther, A, Webber, B, Wendisch, M, Whitehall, S, Wiltshire, A, Wing, A, Wirth, M, Wiskandt, J, Wolf, K, Worbes, L, Wright, E, Wulfmeyer, V, Young, S, Zhang, C, Zhang, D, Ziemen, F, Zinner, T, Zoger, M, Stevens B., Bony S., Farrell D., Ament F., Blyth A., Fairall C., Karstensen J., Quinn P. K., Speich S., Acquistapace C., Aemisegger F., Albright A. L., Bellenger H., Bodenschatz E., Caesar K. -A., Chewitt-Lucas R., De Boer G., Delanoe J., Denby L., Ewald F., Fildier B., Forde M., George G., Gross S., Hagen M., Hausold A., Heywood K. J., Hirsch L., Jacob M., Jansen F., Kinne S., Klocke D., Kolling T., Konow H., Lothon M., Mohr W., Naumann A. K., Nuijens L., Olivier L., Pincus R., Pohlker M., Reverdin G., Roberts G., Schnitt S., Schulz H., Pier Siebesma A., Stephan C. C., Sullivan P., Touze-Peiffer L., Vial J., Vogel R., Zuidema P., Alexander N., Alves L., Arixi S., Asmath H., Bagheri G., Baier K., Bailey A., Baranowski D., Baron A., Barrau S., Barrett P. A., Batier F., Behrendt A., Bendinger A., Beucher F., Bigorre S., Blades E., Blossey P., Bock O., Boing S., Bosser P., Bourras D., Bouruet-Aubertot P., Bower K., Branellec P., Branger H., Brennek M., Brewer A., Brilouet P. -E., Brugmann B., Buehler S. A., Burke E., Burton R., Calmer R., Canonici J. -C., Carton X., Cato G., Charles J. A., Chazette P., Chen Y., Chilinski M. T., Choularton T., Chuang P., Clarke S., Coe H., Cornet C., Coutris P., Couvreux F., Crewell S., Cronin T., Cui Z., Cuypers Y., Daley A., Damerell G. M., Dauhut T., Deneke H., Desbios J. -P., Dorner S., Donner S., Douet V., Drushka K., Dutsch M., Ehrlich A., Emanuel K., Emmanouilidis A., Etienne J. -C., Etienne-Leblanc S., Faure G., Feingold G., Ferrero L., Fix A., Flamant C., Flatau P. J., Foltz G. R., Forster L., Furtuna I., Gadian A., Galewsky J., Gallagher M., Gallimore P., Gaston C., Gentemann C., Geyskens N., Giez A., Gollop J., Gouirand I., Gourbeyre C., De Graaf D., De Groot G. E., Grosz R., Guttler J., Gutleben M., Hall K., Harris G., Helfer K. C., Henze D., Herbert C., Holanda B., Ibanez-Landeta A., Intrieri J., Iyer S., Julien F., Kalesse H., Kazil J., Kellman A., Kidane A. T., Kirchner U., Klingebiel M., Korner M., Kremper L. A., Kretzschmar J., Kruger O., Kumala W., Kurz A., L'Hegaret P., Labaste M., Lachlan-Cope T., Laing A., Landschutzer P., Lang T., Lange D., Lange I., Laplace C., Lavik G., Laxenaire R., LeBihan C., Leandro M., Lefevre N., Lena M., Lenschow D., Li Q., Lloyd G., Los S., Losi N., Lovell O., Luneau C., Makuch P., Malinowski S., Manta G., Marinou E., Marsden N., Masson S., Maury N., Mayer B., Mayers-Als M., Mazel C., McGeary W., McWilliams J. C., Mech M., Mehlmann M., Meroni A. N., Mieslinger T., Minikin A., Minnett P., Moller G., Avalos Y. M., Muller C., Musat I., Napoli A., Neuberger A., Noisel C., Noone D., Nordsiek F., Nowak J. L., Oswald L., Parker D. J., Peck C., Person R., Philippi M., Plueddemann A., Pohlker C., Portge V., Poschl U., Pologne L., Posyniak M., Prange M., Melendez E. Q., Radtke J., Ramage K., Reimann J., Renault L., Reus K., Reyes A., Ribbe J., Ringel M., Ritschel M., Rocha C. B., Rochetin N., Rottenbacher J., Rollo C., Royer H., Sadoulet P., Saffin L., Sandiford S., Sandu I., Schafer M., Schemann V., Schirmacher I., Schlenczek O., Schmidt J., Schroder M., Schwarzenboeck A., Sealy A., Senff C. J., Serikov I., Shohan S., Siddle E., Smirnov A., Spath F., Spooner B., Katharina Stolla M., Szkolka W., De Szoeke S. P., Tarot S., Tetoni E., Thompson E., Thomson J., Tomassini L., Totems J., Ubele A. A., Villiger L., Von Arx J., Wagner T., Walther A., Webber B., Wendisch M., Whitehall S., Wiltshire A., Wing A. A., Wirth M., Wiskandt J., Wolf K., Worbes L., Wright E., Wulfmeyer V., Young S., Zhang C., Zhang D., Ziemen F., Zinner T., Zoger M., Stevens, B, Bony, S, Farrell, D, Ament, F, Blyth, A, Fairall, C, Karstensen, J, Quinn, P, Speich, S, Acquistapace, C, Aemisegger, F, Albright, A, Bellenger, H, Bodenschatz, E, Caesar, K, Chewitt-Lucas, R, De Boer, G, Delanoe, J, Denby, L, Ewald, F, Fildier, B, Forde, M, George, G, Gross, S, Hagen, M, Hausold, A, Heywood, K, Hirsch, L, Jacob, M, Jansen, F, Kinne, S, Klocke, D, Kolling, T, Konow, H, Lothon, M, Mohr, W, Naumann, A, Nuijens, L, Olivier, L, Pincus, R, Pohlker, M, Reverdin, G, Roberts, G, Schnitt, S, Schulz, H, Pier Siebesma, A, Stephan, C, Sullivan, P, Touze-Peiffer, L, Vial, J, Vogel, R, Zuidema, P, Alexander, N, Alves, L, Arixi, S, Asmath, H, Bagheri, G, Baier, K, Bailey, A, Baranowski, D, Baron, A, Barrau, S, Barrett, P, Batier, F, Behrendt, A, Bendinger, A, Beucher, F, Bigorre, S, Blades, E, Blossey, P, Bock, O, Boing, S, Bosser, P, Bourras, D, Bouruet-Aubertot, P, Bower, K, Branellec, P, Branger, H, Brennek, M, Brewer, A, Brilouet, P, Brugmann, B, Buehler, S, Burke, E, Burton, R, Calmer, R, Canonici, J, Carton, X, Cato, G, Charles, J, Chazette, P, Chen, Y, Chilinski, M, Choularton, T, Chuang, P, Clarke, S, Coe, H, Cornet, C, Coutris, P, Couvreux, F, Crewell, S, Cronin, T, Cui, Z, Cuypers, Y, Daley, A, Damerell, G, Dauhut, T, Deneke, H, Desbios, J, Dorner, S, Donner, S, Douet, V, Drushka, K, Dutsch, M, Ehrlich, A, Emanuel, K, Emmanouilidis, A, Etienne, J, Etienne-Leblanc, S, Faure, G, Feingold, G, Ferrero, L, Fix, A, Flamant, C, Flatau, P, Foltz, G, Forster, L, Furtuna, I, Gadian, A, Galewsky, J, Gallagher, M, Gallimore, P, Gaston, C, Gentemann, C, Geyskens, N, Giez, A, Gollop, J, Gouirand, I, Gourbeyre, C, De Graaf, D, De Groot, G, Grosz, R, Guttler, J, Gutleben, M, Hall, K, Harris, G, Helfer, K, Henze, D, Herbert, C, Holanda, B, Ibanez-Landeta, A, Intrieri, J, Iyer, S, Julien, F, Kalesse, H, Kazil, J, Kellman, A, Kidane, A, Kirchner, U, Klingebiel, M, Korner, M, Kremper, L, Kretzschmar, J, Kruger, O, Kumala, W, Kurz, A, L'Hegaret, P, Labaste, M, Lachlan-Cope, T, Laing, A, Landschutzer, P, Lang, T, Lange, D, Lange, I, Laplace, C, Lavik, G, Laxenaire, R, Lebihan, C, Leandro, M, Lefevre, N, Lena, M, Lenschow, D, Li, Q, Lloyd, G, Los, S, Losi, N, Lovell, O, Luneau, C, Makuch, P, Malinowski, S, Manta, G, Marinou, E, Marsden, N, Masson, S, Maury, N, Mayer, B, Mayers-Als, M, Mazel, C, Mcgeary, W, Mcwilliams, J, Mech, M, Mehlmann, M, Meroni, A, Mieslinger, T, Minikin, A, Minnett, P, Moller, G, Avalos, Y, Muller, C, Musat, I, Napoli, A, Neuberger, A, Noisel, C, Noone, D, Nordsiek, F, Nowak, J, Oswald, L, Parker, D, Peck, C, Person, R, Philippi, M, Plueddemann, A, Pohlker, C, Portge, V, Poschl, U, Pologne, L, Posyniak, M, Prange, M, Melendez, E, Radtke, J, Ramage, K, Reimann, J, Renault, L, Reus, K, Reyes, A, Ribbe, J, Ringel, M, Ritschel, M, Rocha, C, Rochetin, N, Rottenbacher, J, Rollo, C, Royer, H, Sadoulet, P, Saffin, L, Sandiford, S, Sandu, I, Schafer, M, Schemann, V, Schirmacher, I, Schlenczek, O, Schmidt, J, Schroder, M, Schwarzenboeck, A, Sealy, A, Senff, C, Serikov, I, Shohan, S, Siddle, E, Smirnov, A, Spath, F, Spooner, B, Katharina Stolla, M, Szkolka, W, De Szoeke, S, Tarot, S, Tetoni, E, Thompson, E, Thomson, J, Tomassini, L, Totems, J, Ubele, A, Villiger, L, Von Arx, J, Wagner, T, Walther, A, Webber, B, Wendisch, M, Whitehall, S, Wiltshire, A, Wing, A, Wirth, M, Wiskandt, J, Wolf, K, Worbes, L, Wright, E, Wulfmeyer, V, Young, S, Zhang, C, Zhang, D, Ziemen, F, Zinner, T, Zoger, M, Stevens B., Bony S., Farrell D., Ament F., Blyth A., Fairall C., Karstensen J., Quinn P. K., Speich S., Acquistapace C., Aemisegger F., Albright A. L., Bellenger H., Bodenschatz E., Caesar K. -A., Chewitt-Lucas R., De Boer G., Delanoe J., Denby L., Ewald F., Fildier B., Forde M., George G., Gross S., Hagen M., Hausold A., Heywood K. J., Hirsch L., Jacob M., Jansen F., Kinne S., Klocke D., Kolling T., Konow H., Lothon M., Mohr W., Naumann A. K., Nuijens L., Olivier L., Pincus R., Pohlker M., Reverdin G., Roberts G., Schnitt S., Schulz H., Pier Siebesma A., Stephan C. C., Sullivan P., Touze-Peiffer L., Vial J., Vogel R., Zuidema P., Alexander N., Alves L., Arixi S., Asmath H., Bagheri G., Baier K., Bailey A., Baranowski D., Baron A., Barrau S., Barrett P. A., Batier F., Behrendt A., Bendinger A., Beucher F., Bigorre S., Blades E., Blossey P., Bock O., Boing S., Bosser P., Bourras D., Bouruet-Aubertot P., Bower K., Branellec P., Branger H., Brennek M., Brewer A., Brilouet P. -E., Brugmann B., Buehler S. A., Burke E., Burton R., Calmer R., Canonici J. -C., Carton X., Cato G., Charles J. A., Chazette P., Chen Y., Chilinski M. T., Choularton T., Chuang P., Clarke S., Coe H., Cornet C., Coutris P., Couvreux F., Crewell S., Cronin T., Cui Z., Cuypers Y., Daley A., Damerell G. M., Dauhut T., Deneke H., Desbios J. -P., Dorner S., Donner S., Douet V., Drushka K., Dutsch M., Ehrlich A., Emanuel K., Emmanouilidis A., Etienne J. -C., Etienne-Leblanc S., Faure G., Feingold G., Ferrero L., Fix A., Flamant C., Flatau P. J., Foltz G. R., Forster L., Furtuna I., Gadian A., Galewsky J., Gallagher M., Gallimore P., Gaston C., Gentemann C., Geyskens N., Giez A., Gollop J., Gouirand I., Gourbeyre C., De Graaf D., De Groot G. E., Grosz R., Guttler J., Gutleben M., Hall K., Harris G., Helfer K. C., Henze D., Herbert C., Holanda B., Ibanez-Landeta A., Intrieri J., Iyer S., Julien F., Kalesse H., Kazil J., Kellman A., Kidane A. T., Kirchner U., Klingebiel M., Korner M., Kremper L. A., Kretzschmar J., Kruger O., Kumala W., Kurz A., L'Hegaret P., Labaste M., Lachlan-Cope T., Laing A., Landschutzer P., Lang T., Lange D., Lange I., Laplace C., Lavik G., Laxenaire R., LeBihan C., Leandro M., Lefevre N., Lena M., Lenschow D., Li Q., Lloyd G., Los S., Losi N., Lovell O., Luneau C., Makuch P., Malinowski S., Manta G., Marinou E., Marsden N., Masson S., Maury N., Mayer B., Mayers-Als M., Mazel C., McGeary W., McWilliams J. C., Mech M., Mehlmann M., Meroni A. N., Mieslinger T., Minikin A., Minnett P., Moller G., Avalos Y. M., Muller C., Musat I., Napoli A., Neuberger A., Noisel C., Noone D., Nordsiek F., Nowak J. L., Oswald L., Parker D. J., Peck C., Person R., Philippi M., Plueddemann A., Pohlker C., Portge V., Poschl U., Pologne L., Posyniak M., Prange M., Melendez E. Q., Radtke J., Ramage K., Reimann J., Renault L., Reus K., Reyes A., Ribbe J., Ringel M., Ritschel M., Rocha C. B., Rochetin N., Rottenbacher J., Rollo C., Royer H., Sadoulet P., Saffin L., Sandiford S., Sandu I., Schafer M., Schemann V., Schirmacher I., Schlenczek O., Schmidt J., Schroder M., Schwarzenboeck A., Sealy A., Senff C. J., Serikov I., Shohan S., Siddle E., Smirnov A., Spath F., Spooner B., Katharina Stolla M., Szkolka W., De Szoeke S. P., Tarot S., Tetoni E., Thompson E., Thomson J., Tomassini L., Totems J., Ubele A. A., Villiger L., Von Arx J., Wagner T., Walther A., Webber B., Wendisch M., Whitehall S., Wiltshire A., Wing A. A., Wirth M., Wiskandt J., Wolf K., Worbes L., Wright E., Wulfmeyer V., Young S., Zhang C., Zhang D., Ziemen F., Zinner T., and Zoger M.

Abstract

The science guiding the EUREC4A campaign and its measurements is presented. EUREC4A comprised roughly 5 weeks of measurements in the downstream winter trades of the North Atlantic - eastward and southeastward of Barbados. Through its ability to characterize processes operating across a wide range of scales, EUREC4A marked a turning point in our ability to observationally study factors influencing clouds in the trades, how they will respond to warming, and their link to other components of the earth system, such as upper-ocean processes or the life cycle of particulate matter. This characterization was made possible by thousands (2500) of sondes distributed to measure circulations on meso- (200 km) and larger (500 km) scales, roughly 400 h of flight time by four heavily instrumented research aircraft; four global-class research vessels; an advanced ground-based cloud observatory; scores of autonomous observing platforms operating in the upper ocean (nearly 10 000 profiles), lower atmosphere (continuous profiling), and along the air-sea interface; a network of water stable isotopologue measurements; targeted tasking of satellite remote sensing; and modeling with a new generation of weather and climate models. In addition to providing an outline of the novel measurements and their composition into a unified and coordinated campaign, the six distinct scientific facets that EUREC4A explored - from North Brazil Current rings to turbulence-induced clustering of cloud droplets and its influence on warm-rain formation - are presented along with an overview of EUREC4A's outreach activities, environmental impact, and guidelines for scientific practice. Track data for all platforms are standardized and accessible at 10.25326/165 , and a film documenting the campaign is provided as a video supplement.

Published
2021

2. EUREC 4 A

Author

Stevens, Bjorn, Bony, Sandrine, Farrell, David, Ament, Felix, Blyth, Alan M., Fairall, Chiristopher W., Karstensen, Johannes, Quinn, Patricia K., Speich, Sabrina, Acquistapace, Claudia, Aemisegger, Franziska, Albright, Anna, Bellenger, Hugo, Bodenschatz, Eberhard, Caesar, Kathy-Ann, Chewitt-Lucas, Rebecca, De Boer, Gijs, Delanoë, Julien, Denby, Leif Christopher, Ewald, Florian, Fildier, Benjamin, Forde, Marvin, George, Geet, Gross, Silke, Hagen, Martin, Hausold, Andrea, Heywood, Karen J., Hirsch, Lutz, Jacob, Marek, Jansen, Friedhelm, Kinne, Stefan, Klocke, Daniel, Kölling, Tobias, Konow, Heike, Lothon, Marie, Mohr, Wiebke, Naumann, Ann Kristin, Nuijens, Louise, Olivier, Léa, Pincus, Robert, Pöhlker, Mira L., Reverdin, Gilles, Roberts, Gregory, Schnitt, Sabrina, Sullivan, Peter P., Touzé-Peiffer, Ludovic, Vial, Jessica, Vogel, Raphaela, Alexander, Nicola, Alves, Lyndon, Arixi, Sophian, Asmath, Hamish, Bagheri, Gholamhossein, Bailey, Adriana, Baranowski, Dariusz, Baron, Alexandre, Barrau, Sébastien, Barrett, Paul Alan, Behrendt, Andreas, Bendinger, Arne, Beucher, Florent, Bigorre, Sebastien, Bosser, Pierre, Blossey, P., Bock, Olivier, Bourras, Denis, Bouruet-Aubertot, Pascale, Bower, K, Branger, H, Brennek, M, Brewer, A, Brilouet, P.-E, Brügmann, B, Buehler, S, Burke, E, Burton, R, Böing, S, Calmer, R, Canonici, J.-C, Carton, X, Cato, G, Charles, J, Chazette, Patrick, Chen, Y, Choularton, T, Chuang, P, Clarke, S, Coe, H, Cornet, C, Coutris, P, Couvreux, F, Crewell, S, Cronin, T, Cui, Z, Cuypers, Y, Daley, A, Damerell, G, Dauhut, T, De Graaf, D, De Groot, G, De Szoeke, S, Deneke, H, Desbios, J.-P, Douet, V, Drushka, K, Dütsch, M, Ehrlich, A, Emanuel, K, Emmanouilidis, A, Etienne, J.-C, Etienne-Leblanc, S, Faure, G, Feingold, G, Ferrero, L, Fix, A, Flamant, Cyrille, Flatau, P, Foltz, G, Gadian, A, Galewsky, J, Gallagher, M, Gallimore, P, Gaston, C, Gentemann, C, Geyskens, N, Giez, A, Gourbeyre, C, Grosz, R, Gutleben, M, Güttler, J, Hall, K, Harris, G, Helfer, K, Henze, D, Herbert, C, Holanda, B, Ibanez-Landeta, A, Intrieri, J, Iyer, S, Julien, F, Kalesse, H, Kazil, J, Kellman, A, Kirchner, U, Klingebiel, M, Kremper, L, Kretzschmar, J, Krüger, O, Kurz, A, Körner, M, L'hégaret, P, Lachlan-Cope, T, Laing, A, Landschützer, P, Lang, T, Lange, D, Lange, I, Laplace, C, Laxenaire, R, Le Bihan, C, Leandro, M, Lefevre, N, Lenschow, D, Li, Q, Lloyd, G, Los, S, Losi, N, Lovell, O, Luneau, C, Makuch, P, Malinowski, S, Manta, G, Marinou, E, Marsden, N, Matthieu, L, Maury, N, Mayer, B, Mayers-Als, M, Mazel, Christophe, Mcgeary, W, Mcwilliams, J, Mech, M, Mehlmann, M, Meroni, A, Mieslinger, T, Minikin, A, Avalos, Y, Muller, Caroline, Musat, I, Möller, G, Napoli, A, Neuberger, A, Noone, D, Nordsiek, F, Nowak, J, Oswald, L, Parker, D, Peck, C, Person, R, Plueddemann, A, Pologne, L, Posyniak, M, Prange, M, Pöhlker, C, Pörtge, V, Pöschl, U, Quiñones Meléndez, E, Radtke, J, Ramage, K, Reimann, J, Renault, L, Reus, K, Reyes, A, Ribbe, J, Ringel, M, Ritschel, M, Rocha, C, Rochetin, N, Rollo, C, Röttenbacher, J, Saffin, L, Sandiford, S, Sandu, I, Schemann, V, Schirmacher, I, Schlenczek, O, Schmidt, J, Schröder, M, Schulz, H, Schwarzenboeck, A, Schäfer, M, Sealy, A, Serikov, I, Shohan, S, Siddle, E, Siebesma, A, Späth, F, Stephan, C, Stolla, M, Szkółka, W, Tarot, S, Tetoni, E, Thompson, E, Thomson, J, Tomassini, L, Totems, J, Villiger, L, Walther, A, Webber, B, Wendisch, M, Whitehall, S, Wiltshire, A, Wing, A, Wirth, M, Wiskandt, J, Wolf, K, Worbes, L, Wright, E, Wulfmeyer, V, Young, S, Zhang, D, Zhang, C, Ziemen, F, Zinner, T, Zuidema, P, Zöger, M, Max Planck Institute for Meteorology (MPI-M), Max-Planck-Gesellschaft, Laboratoire de Météorologie Dynamique (UMR 8539) (LMD), Institut national des sciences de l'Univers (INSU - CNRS)-École polytechnique (X)-École des Ponts ParisTech (ENPC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Département des Géosciences - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Caribbean Institute for Meteorology and Hydrology (CIMH), Meteorological Institute [Hamburg], University of Hamburg, National Centre for Atmospheric Science [Leeds] (NCAS), Natural Environment Research Council (NERC), NOAA Earth System Research Laboratory (ESRL), National Oceanic and Atmospheric Administration (NOAA), Helmholtz Centre for Ocean Research [Kiel] (GEOMAR), NOAA Pacific Marine Environmental Laboratory [Seattle] (PMEL), Institute for Geophysics and Meteorology [Köln] (IGM), University of Cologne, Institute for Atmospheric and Climate Science [Zürich] (IAC), Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology in Zürich [Zürich] (ETH Zürich), Max Planck Institute for Dynamics and Self-Organization (MPIDS), NOAA Physical Sciences Laboratory (PSL), SPACE - LATMOS, Laboratoire Atmosphères, Milieux, Observations Spatiales (LATMOS), Sorbonne Université (SU)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS), University of Leeds, Deutsches Zentrum für Luft- und Raumfahrt (DLR), Deutscher Wetterdienst [Offenbach] (DWD), Ludwig-Maximilians-Universität München (LMU), Laboratoire d'aérologie (LA), Centre National de la Recherche Scientifique (CNRS)-Observatoire Midi-Pyrénées (OMP), Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées, Universität Hamburg (UHH), Max Planck Institute for Marine Microbiology, Delft University of Technology (TU Delft), Laboratoire d'Océanographie et du Climat : Expérimentations et Approches Numériques (LOCEAN), Sorbonne Université (SU)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Institut Pierre-Simon-Laplace (IPSL (FR_636)), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Universität zu Köln, École nationale des sciences géographiques (ENSG), Institut National de l'Information Géographique et Forestière [IGN] (IGN)-Université Gustave Eiffel, Institut de Physique du Globe de Paris (IPGP), Institut national des sciences de l'Univers (INSU - CNRS)-IPG PARIS-Université de La Réunion (UR)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), National Center for Atmospheric Research [Boulder] (NCAR), Institut de Recherche sur les Phénomènes Hors Equilibre (IRPHE), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), TROPO - LATMOS, Institut Pythéas (OSU PYTHEAS), and Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
[SDE]Environmental Sciences, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2020

6. TFIIIB placement on a yeast U6 RNA gene in vivo is directed primarily by TFIIIC rather than by sequence-specific DNA contacts

Author

Gerlach, V L, Whitehall, S K, Geiduschek, E P, and Brow, D A

Abstract

The Saccharomyces cerevisiae U6 RNA gene (SNR6), which is transcribed by RNA polymerase III, has an unusual combination of promoter elements: an upstream TATA box, an intragenic A block, and a downstream B block. In tRNA genes, the A and B blocks are binding sites for the transcription initiation factor TFIIIC, which positions TFIIIB a fixed distance upstream of the A block. However, in vitro transcription of SNR6 with purified components requires neither TFIIIC nor the A and B blocks, presumably because TFIIIB recognizes the upstream sequences directly. Here we demonstrate that TFIIIB placement on SNR6 in vivo is directed primarily by the TFIIIC-binding elements rather than by upstream sequences. We show that the A block is a stronger start site determinant than the upstream sequences when the two are uncoupled by an insertion mutation. Furthermore, while TFIIIC-independent in vitro transcription of SNR6 is highly sensitive to TATA box point mutations, in vivo initiation on SNR6 is only marginally sensitive to such mutations unless the A block is mutated. Intriguingly, a deletion downstream of the U6 RNA coding region that reduces A-to-B block spacing also increases in vivo dependence on the TATA box. Moreover, this deletion results in the appearance of micrococcal nuclease-hypersensitive sites in the TFIIIB chromatin footprint, indicating that TFIIIB binding is disrupted by a mutation 150 bp distant. This and additional chromatin footprinting data suggest that SNR6 is assembled into a nucleoprotein complex that facilitates the TFIIIC-dependent binding of TFIIIB.

Published
1995
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8. EUREC4A

Author

B. Stevens, S. Bony, D. Farrell, F. Ament, A. Blyth, C. Fairall, J. Karstensen, P. K. Quinn, S. Speich, C. Acquistapace, F. Aemisegger, A. L. Albright, H. Bellenger, E. Bodenschatz, K.-A. Caesar, R. Chewitt-Lucas, G. de Boer, J. Delanoë, L. Denby, F. Ewald, B. Fildier, M. Forde, G. George, S. Gross, M. Hagen, A. Hausold, K. J. Heywood, L. Hirsch, M. Jacob, F. Jansen, S. Kinne, D. Klocke, T. Kölling, H. Konow, M. Lothon, W. Mohr, A. K. Naumann, L. Nuijens, L. Olivier, R. Pincus, M. Pöhlker, G. Reverdin, G. Roberts, S. Schnitt, H. Schulz, A. P. Siebesma, C. C. Stephan, P. Sullivan, L. Touzé-Peiffer, J. Vial, R. Vogel, P. Zuidema, N. Alexander, L. Alves, S. Arixi, H. Asmath, G. Bagheri, K. Baier, A. Bailey, D. Baranowski, A. Baron, S. Barrau, P. A. Barrett, F. Batier, A. Behrendt, A. Bendinger, F. Beucher, S. Bigorre, E. Blades, P. Blossey, O. Bock, S. Böing, P. Bosser, D. Bourras, P. Bouruet-Aubertot, K. Bower, P. Branellec, H. Branger, M. Brennek, A. Brewer, P.-E. Brilouet, B. Brügmann, S. A. Buehler, E. Burke, R. Burton, R. Calmer, J.-C. Canonici, X. Carton, G. Cato Jr., J. A. Charles, P. Chazette, Y. Chen, M. T. Chilinski, T. Choularton, P. Chuang, S. Clarke, H. Coe, C. Cornet, P. Coutris, F. Couvreux, S. Crewell, T. Cronin, Z. Cui, Y. Cuypers, A. Daley, G. M. Damerell, T. Dauhut, H. Deneke, J.-P. Desbios, S. Dörner, S. Donner, V. Douet, K. Drushka, M. Dütsch, A. Ehrlich, K. Emanuel, A. Emmanouilidis, J.-C. Etienne, S. Etienne-Leblanc, G. Faure, G. Feingold, L. Ferrero, A. Fix, C. Flamant, P. J. Flatau, G. R. Foltz, L. Forster, I. Furtuna, A. Gadian, J. Galewsky, M. Gallagher, P. Gallimore, C. Gaston, C. Gentemann, N. Geyskens, A. Giez, J. Gollop, I. Gouirand, C. Gourbeyre, D. de Graaf, G. E. de Groot, R. Grosz, J. Güttler, M. Gutleben, K. Hall, G. Harris, K. C. Helfer, D. Henze, C. Herbert, B. Holanda, A. Ibanez-Landeta, J. Intrieri, S. Iyer, F. Julien, H. Kalesse, J. Kazil, A. Kellman, A. T. Kidane, U. Kirchner, M. Klingebiel, M. Körner, L. A. Kremper, J. Kretzschmar, O. Krüger, W. Kumala, A. Kurz, P. L'Hégaret, M. Labaste, T. Lachlan-Cope, A. Laing, P. Landschützer, T. Lang, D. Lange, I. Lange, C. Laplace, G. Lavik, R. Laxenaire, C. Le Bihan, M. Leandro, N. Lefevre, M. Lena, D. Lenschow, Q. Li, G. Lloyd, S. Los, N. Losi, O. Lovell, C. Luneau, P. Makuch, S. Malinowski, G. Manta, E. Marinou, N. Marsden, S. Masson, N. Maury, B. Mayer, M. Mayers-Als, C. Mazel, W. McGeary, J. C. McWilliams, M. Mech, M. Mehlmann, A. N. Meroni, T. Mieslinger, A. Minikin, P. Minnett, G. Möller, Y. Morfa Avalos, C. Muller, I. Musat, A. Napoli, A. Neuberger, C. Noisel, D. Noone, F. Nordsiek, J. L. Nowak, L. Oswald, D. J. Parker, C. Peck, R. Person, M. Philippi, A. Plueddemann, C. Pöhlker, V. Pörtge, U. Pöschl, L. Pologne, M. Posyniak, M. Prange, E. Quiñones Meléndez, J. Radtke, K. Ramage, J. Reimann, L. Renault, K. Reus, A. Reyes, J. Ribbe, M. Ringel, M. Ritschel, C. B. Rocha, N. Rochetin, J. Röttenbacher, C. Rollo, H. Royer, P. Sadoulet, L. Saffin, S. Sandiford, I. Sandu, M. Schäfer, V. Schemann, I. Schirmacher, O. Schlenczek, J. Schmidt, M. Schröder, A. Schwarzenboeck, A. Sealy, C. J. Senff, I. Serikov, S. Shohan, E. Siddle, A. Smirnov, F. Späth, B. Spooner, M. K. Stolla, W. Szkółka, S. P. de Szoeke, S. Tarot, E. Tetoni, E. Thompson, J. Thomson, L. Tomassini, J. Totems, A. A. Ubele, L. Villiger, J. von Arx, T. Wagner, A. Walther, B. Webber, M. Wendisch, S. Whitehall, A. Wiltshire, A. A. Wing, M. Wirth, J. Wiskandt, K. Wolf, L. Worbes, E. Wright, V. Wulfmeyer, S. Young, C. Zhang, D. Zhang, F. Ziemen, T. Zinner, M. Zöger, Max Planck Institute for Meteorology (MPI-M), Max-Planck-Gesellschaft, Laboratoire de Météorologie Dynamique (UMR 8539) (LMD), Institut national des sciences de l'Univers (INSU - CNRS)-École polytechnique (X)-École des Ponts ParisTech (ENPC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Département des Géosciences - ENS Paris, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Caribbean Institute for Meteorology and Hydrology (CIMH), Meteorological Institute [Hamburg], University of Hamburg, National Centre for Atmospheric Science [Leeds] (NCAS), Natural Environment Research Council (NERC), NOAA Earth System Research Laboratory (ESRL), National Oceanic and Atmospheric Administration (NOAA), Helmholtz Centre for Ocean Research [Kiel] (GEOMAR), NOAA Pacific Marine Environmental Laboratory [Seattle] (PMEL), Institute for Geophysics and Meteorology [Köln] (IGM), University of Cologne, Institute for Atmospheric and Climate Science [Zürich] (IAC), Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), Max Planck Institute for Dynamics and Self-Organization (MPIDS), NOAA Physical Sciences Laboratory (PSL), SPACE - LATMOS, Laboratoire Atmosphères, Milieux, Observations Spatiales (LATMOS), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), University of Leeds, Deutsches Zentrum für Luft- und Raumfahrt (DLR), Deutscher Wetterdienst [Offenbach] (DWD), Ludwig-Maximilians-Universität München (LMU), Laboratoire d'aérologie (LAERO), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Observatoire Midi-Pyrénées (OMP), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Centre National de la Recherche Scientifique (CNRS), Universität Hamburg (UHH), Max Planck Institute for Marine Microbiology, Delft University of Technology (TU Delft), Processus et interactions de fine échelle océanique (PROTEO), Laboratoire d'Océanographie et du Climat : Expérimentations et Approches Numériques (LOCEAN), Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut Pierre-Simon-Laplace (IPSL (FR_636)), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut Pierre-Simon-Laplace (IPSL (FR_636)), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Cooperative Institute for Research in Environmental Sciences (CIRES), University of Colorado [Boulder]-National Oceanic and Atmospheric Administration (NOAA), Max-Planck-Institut für Chemie (MPIC), Centre national de recherches météorologiques (CNRM), Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Midi-Pyrénées (OMP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Centre National de la Recherche Scientifique (CNRS), Scripps Institution of Oceanography (SIO - UC San Diego), University of California [San Diego] (UC San Diego), University of California (UC)-University of California (UC), Universität zu Köln = University of Cologne, National Center for Atmospheric Research [Boulder] (NCAR), Hydrometeorological Service [Georgetown], Ministry of Agriculture [Guyana], Institute of Marine Affairs (IMA), Institute of Geophysics [Warsaw], Polska Akademia Nauk = Polish Academy of Sciences (PAN), Laboratoire des Sciences du Climat et de l'Environnement [Gif-sur-Yvette] (LSCE), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), United Kingdom Met Office [Exeter], Institute of Physics and Meteorology [Stuttgart] (IPM), University of Hohenheim, Woods Hole Oceanographic Institution (WHOI), Queen Elizabeth Hospital [Bridgetown, St. Michael, Barbados] (QEH), Department of Atmospheric Sciences [Seattle], University of Washington [Seattle], École nationale des sciences géographiques (ENSG), Institut National de l'Information Géographique et Forestière [IGN] (IGN)-Université Gustave Eiffel, Institut de Physique du Globe de Paris (IPGP (UMR_7154)), Institut national des sciences de l'Univers (INSU - CNRS)-Université de La Réunion (UR)-Institut de Physique du Globe de Paris (IPG Paris)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Equipe Marine Mapping & Metrology (Lab-STICC_M3), Laboratoire des sciences et techniques de l'information, de la communication et de la connaissance (Lab-STICC), École Nationale d'Ingénieurs de Brest (ENIB)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL)-IMT Atlantique (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-École Nationale d'Ingénieurs de Brest (ENIB)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL)-IMT Atlantique (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT), École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne), University of Manchester [Manchester], Institut de Recherche sur les Phénomènes Hors Equilibre (IRPHE), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), University of Warsaw (UW), NOAA Chemical Sciences Laboratory (CSL), Max-Planck-Institut für Meteorologie (MPI-M), St. Christopher Air and Sea Port (SCASPA), Service des Avions Français Instrumentés pour la Recherche en Environnement (SAFIRE), Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France, Laboratoire d'Océanographie Physique et Spatiale (LOPS), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), Saint Vincent and the Grenadines Meteorological Services, Grenada Meteorological Services, Chimie Atmosphérique Expérimentale (CAE), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut Pierre-Simon-Laplace (IPSL (FR_636)), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), University of California [Santa Cruz] (UC Santa Cruz), University of California (UC), Cayman Islands National Weather Service, Laboratoire d’Optique Atmosphérique - UMR 8518 (LOA), Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Météorologie Physique (LaMP), Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA), Massachusetts Institute of Technology (MIT), Centre for Ocean and Atmospheric Sciences [Norwich] (COAS), School of Environmental Sciences [Norwich], University of East Anglia [Norwich] (UEA)-University of East Anglia [Norwich] (UEA), College of Earth, Ocean and Atmospheric Sciences [Corvallis] (CEOAS), Oregon State University (OSU), Leibniz Institute for Tropospheric Research (TROPOS), Department of Meteorology and Geophysics [Vienna], Universität Wien, Leipziger Institut für Meteorologie (LIM), Universität Leipzig, Meteorological Department (St. Maarten), Università degli Studi di Milano-Bicocca = University of Milano-Bicocca (UNIMIB), TROPO - LATMOS, NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML), Department of Earth and Planetary Sciences [Albuquerque] (EPS), The University of New Mexico [Albuquerque], University of Miami [Coral Gables], Farallon Institute, Division technique INSU/SDU (DTI), Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Deutsches Zentrum für Luft- und Raumfahrt [Oberpfaffenhofen-Wessling] (DLR), Regional Security System [Barbados], Max Planck Institute for Chemistry (MPIC), Applied Physics Laboratory [Seattle] (APL-UW), Barbados Coast Guard, Développement Instrumental et Techniques Marines (DITM), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Austral, Boréal et Carbone (ABC), Institut Pythéas (OSU PYTHEAS), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Nucleus for European Modeling of the Ocean (NEMO R&D ), Laboratoire d'études en Géophysique et océanographie spatiales (LEGOS), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Midi-Pyrénées (OMP), European Project: 694768,EUREC4A, European Project: 820829,CONSTRAIN H2020, European Project: 741120,COMPASS, European Project: 817578,TRIATLAS(2019), École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Sorbonne Université (SU)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS), Laboratoire d'aérologie (LA), Centre National de la Recherche Scientifique (CNRS)-Observatoire Midi-Pyrénées (OMP), Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées, Sorbonne Université (SU)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Institut Pierre-Simon-Laplace (IPSL (FR_636)), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Sorbonne Université (SU)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Institut Pierre-Simon-Laplace (IPSL (FR_636)), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Institut national des sciences de l'Univers (INSU - CNRS)-Météo France-Centre National de la Recherche Scientifique (CNRS), Scripps Institution of Oceanography (SIO), University of California-University of California, Universität zu Köln, Queen Elizabeth Hospital [Bridgetown, St. Michael, Barbados], Institut de Physique du Globe de Paris (IPGP), Institut national des sciences de l'Univers (INSU - CNRS)-IPG PARIS-Université de La Réunion (UR)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Institut Mines-Télécom [Paris] (IMT)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-École Nationale d'Ingénieurs de Brest (ENIB)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut de Recherche pour le Développement (IRD)-Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut Pierre-Simon-Laplace (IPSL (FR_636)), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut de Recherche pour le Développement (IRD)-Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU), Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), École Nationale d'Ingénieurs de Brest (ENIB)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-École Nationale d'Ingénieurs de Brest (ENIB)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM), Centre National de la Recherche Scientifique (CNRS)-Météo France-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), University of California [Santa Cruz] (UCSC), University of California, Universität Leipzig [Leipzig], Università degli Studi di Milano-Bicocca [Milano] (UNIMIB), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Midi-Pyrénées (OMP), Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Centre National de la Recherche Scientifique (CNRS), Stevens, B, Bony, S, Farrell, D, Ament, F, Blyth, A, Fairall, C, Karstensen, J, Quinn, P, Speich, S, Acquistapace, C, Aemisegger, F, Albright, A, Bellenger, H, Bodenschatz, E, Caesar, K, Chewitt-Lucas, R, De Boer, G, Delanoe, J, Denby, L, Ewald, F, Fildier, B, Forde, M, George, G, Gross, S, Hagen, M, Hausold, A, Heywood, K, Hirsch, L, Jacob, M, Jansen, F, Kinne, S, Klocke, D, Kolling, T, Konow, H, Lothon, M, Mohr, W, Naumann, A, Nuijens, L, Olivier, L, Pincus, R, Pohlker, M, Reverdin, G, Roberts, G, Schnitt, S, Schulz, H, Pier Siebesma, A, Stephan, C, Sullivan, P, Touze-Peiffer, L, Vial, J, Vogel, R, Zuidema, P, Alexander, N, Alves, L, Arixi, S, Asmath, H, Bagheri, G, Baier, K, Bailey, A, Baranowski, D, Baron, A, Barrau, S, Barrett, P, Batier, F, Behrendt, A, Bendinger, A, Beucher, F, Bigorre, S, Blades, E, Blossey, P, Bock, O, Boing, S, Bosser, P, Bourras, D, Bouruet-Aubertot, P, Bower, K, Branellec, P, Branger, H, Brennek, M, Brewer, A, Brilouet, P, Brugmann, B, Buehler, S, Burke, E, Burton, R, Calmer, R, Canonici, J, Carton, X, Cato, G, Charles, J, Chazette, P, Chen, Y, Chilinski, M, Choularton, T, Chuang, P, Clarke, S, Coe, H, Cornet, C, Coutris, P, Couvreux, F, Crewell, S, Cronin, T, Cui, Z, Cuypers, Y, Daley, A, Damerell, G, Dauhut, T, Deneke, H, Desbios, J, Dorner, S, Donner, S, Douet, V, Drushka, K, Dutsch, M, Ehrlich, A, Emanuel, K, Emmanouilidis, A, Etienne, J, Etienne-Leblanc, S, Faure, G, Feingold, G, Ferrero, L, Fix, A, Flamant, C, Flatau, P, Foltz, G, Forster, L, Furtuna, I, Gadian, A, Galewsky, J, Gallagher, M, Gallimore, P, Gaston, C, Gentemann, C, Geyskens, N, Giez, A, Gollop, J, Gouirand, I, Gourbeyre, C, De Graaf, D, De Groot, G, Grosz, R, Guttler, J, Gutleben, M, Hall, K, Harris, G, Helfer, K, Henze, D, Herbert, C, Holanda, B, Ibanez-Landeta, A, Intrieri, J, Iyer, S, Julien, F, Kalesse, H, Kazil, J, Kellman, A, Kidane, A, Kirchner, U, Klingebiel, M, Korner, M, Kremper, L, Kretzschmar, J, Kruger, O, Kumala, W, Kurz, A, L'Hegaret, P, Labaste, M, Lachlan-Cope, T, Laing, A, Landschutzer, P, Lang, T, Lange, D, Lange, I, Laplace, C, Lavik, G, Laxenaire, R, Lebihan, C, Leandro, M, Lefevre, N, Lena, M, Lenschow, D, Li, Q, Lloyd, G, Los, S, Losi, N, Lovell, O, Luneau, C, Makuch, P, Malinowski, S, Manta, G, Marinou, E, Marsden, N, Masson, S, Maury, N, Mayer, B, Mayers-Als, M, Mazel, C, Mcgeary, W, Mcwilliams, J, Mech, M, Mehlmann, M, Meroni, A, Mieslinger, T, Minikin, A, Minnett, P, Moller, G, Avalos, Y, Muller, C, Musat, I, Napoli, A, Neuberger, A, Noisel, C, Noone, D, Nordsiek, F, Nowak, J, Oswald, L, Parker, D, Peck, C, Person, R, Philippi, M, Plueddemann, A, Pohlker, C, Portge, V, Poschl, U, Pologne, L, Posyniak, M, Prange, M, Melendez, E, Radtke, J, Ramage, K, Reimann, J, Renault, L, Reus, K, Reyes, A, Ribbe, J, Ringel, M, Ritschel, M, Rocha, C, Rochetin, N, Rottenbacher, J, Rollo, C, Royer, H, Sadoulet, P, Saffin, L, Sandiford, S, Sandu, I, Schafer, M, Schemann, V, Schirmacher, I, Schlenczek, O, Schmidt, J, Schroder, M, Schwarzenboeck, A, Sealy, A, Senff, C, Serikov, I, Shohan, S, Siddle, E, Smirnov, A, Spath, F, Spooner, B, Katharina Stolla, M, Szkolka, W, De Szoeke, S, Tarot, S, Tetoni, E, Thompson, E, Thomson, J, Tomassini, L, Totems, J, Ubele, A, Villiger, L, Von Arx, J, Wagner, T, Walther, A, Webber, B, Wendisch, M, Whitehall, S, Wiltshire, A, Wing, A, Wirth, M, Wiskandt, J, Wolf, K, Worbes, L, Wright, E, Wulfmeyer, V, Young, S, Zhang, C, Zhang, D, Ziemen, F, Zinner, T, Zoger, M, and Institut Mines-Télécom [Paris] (IMT)-École Nationale d'Ingénieurs de Brest (ENIB)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL)-Institut Mines-Télécom [Paris] (IMT)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique)

Subjects
[SDU.OCEAN]Sciences of the Universe [physics]/Ocean, Atmosphere, QE1-996.5, [SDU.STU.GP]Sciences of the Universe [physics]/Earth Sciences/Geophysics [physics.geo-ph], Cloud Feedback, Geology, [SDU.STU.ME]Sciences of the Universe [physics]/Earth Sciences/Meteorology, Environmental sciences, Atmospheric circulation, Trade-wind cumulus, Field campaign, Aerosol, clouds, sea, sub-tropic, CHIM/12 - CHIMICA DELL'AMBIENTE E DEI BENI CULTURALI, [SDE]Environmental Sciences, GE1-350, Shallow convection, [SDU.OTHER]Sciences of the Universe [physics]/Other, ComputingMilieux_MISCELLANEOUS
Abstract

The science guiding the EUREC4A campaign and its measurements is presented. EUREC4A comprised roughly 5 weeks of measurements in the downstream winter trades of the North Atlantic – eastward and southeastward of Barbados. Through its ability to characterize processes operating across a wide range of scales, EUREC4A marked a turning point in our ability to observationally study factors influencing clouds in the trades, how they will respond to warming, and their link to other components of the earth system, such as upper-ocean processes or the life cycle of particulate matter. This characterization was made possible by thousands (2500) of sondes distributed to measure circulations on meso- (200 km) and larger (500 km) scales, roughly 400 h of flight time by four heavily instrumented research aircraft; four global-class research vessels; an advanced ground-based cloud observatory; scores of autonomous observing platforms operating in the upper ocean (nearly 10 000 profiles), lower atmosphere (continuous profiling), and along the air–sea interface; a network of water stable isotopologue measurements; targeted tasking of satellite remote sensing; and modeling with a new generation of weather and climate models. In addition to providing an outline of the novel measurements and their composition into a unified and coordinated campaign, the six distinct scientific facets that EUREC4A explored – from North Brazil Current rings to turbulence-induced clustering of cloud droplets and its influence on warm-rain formation – are presented along with an overview of EUREC4A's outreach activities, environmental impact, and guidelines for scientific practice. Track data for all platforms are standardized and accessible at https://doi.org/10.25326/165 (Stevens, 2021), and a film documenting the campaign is provided as a video supplement.

Published
2021

9. Analysis of Heterochromatin in Schizosaccharomyces pombe.

Author

Cam HP and Whitehall S

Subjects
Heterochromatin genetics, Heterochromatin metabolism, Schizosaccharomyces genetics, Schizosaccharomyces metabolism
Abstract

This introduction briefly describes the biology of heterochromatin in the fission yeast Schizosaccharomyces pombe We highlight some of the salient features of fission yeast that render it an excellent unicellular eukaryote for studying heterochromatin. We then discuss key aspects of heterochromatin that are of interest to those in the field, and last we introduce experimental approaches often used to investigate heterochromatin., (© 2016 Cold Spring Harbor Laboratory Press.)

Published
2016
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10. Micrococcal Nuclease Digestion of Schizosaccharomyces pombe Chromatin.

Author

Cam HP and Whitehall S

Subjects
Hydrolysis, Schizosaccharomyces chemistry, Chromatin metabolism, Micrococcal Nuclease metabolism, Nucleosomes chemistry, Schizosaccharomyces genetics
Abstract

Digestion of chromatin with micrococcal nuclease (MNase) is widely used to probe nucleosome organization. Analysis of MNase digests by end-labeling techniques or overlapping quantitative polymerase chain reaction (qPCR) can be used to map locus-specific nucleosome positions. Furthermore, the application of genomic technologies can provide genome-wide views of nucleosome position and occupancy. This protocol provides a basic method for MNase digestion of Schizosaccharomyces pombe chromatin and depends on the production of permeabilized spheroplasts., (© 2016 Cold Spring Harbor Laboratory Press.)

Published
2016
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11. Chromatin Immunoprecipitation (ChIP) in Schizosaccharomyces pombe.

Author

Cam HP and Whitehall S

Subjects
Binding Sites, Polymerase Chain Reaction, Sequence Analysis, DNA, Chromatin Immunoprecipitation methods, DNA, Fungal metabolism, DNA-Binding Proteins analysis, Schizosaccharomyces chemistry, Schizosaccharomyces genetics
Abstract

Chromatin immunoprecipitation (ChIP), the cross-linking of chromatin followed by immunoprecipitation with antibodies against a chromatin target, is a key method for measuring association of proteins with a specific genomic region(s). As a negative control, a mock ChIP experiment in which no antibody is added to the immunoprecipitation reaction is included. Enriched DNA fragments from a ChIP experiment can be analyzed in a variety of ways. For semiquantitative analysis, a region of interest can be amplified using standard polymerase chain reaction (PCR) techniques. PCR products are analyzed on agarose (or polyacrylamide) gels and band intensity calculated with a standard imaging software. ChIP enrichment is usually calculated as the ratio of ChIP to input compared with a similar ratio for a reference region not expected to be enriched for that factor. For heterochromatin analysis, housekeeping genes such as act1 + are good references. Real-time quantitative PCR (qPCR) can be used for a fully quantitative approach. If a factor is to be mapped across the genome, ChIP DNA can be amplified and labeled for microarray analysis or scrutinized on a next-generation DNA sequencing platform., (© 2016 Cold Spring Harbor Laboratory Press.)

Published
2016
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12. Reporter Gene Silencing Assays in Fission Yeast.

Author

Cam HP and Whitehall S

Subjects
Culture Media chemistry, Molecular Biology methods, Gene Silencing, Genes, Fungal, Genes, Reporter, Genetics, Microbial methods, Heterochromatin metabolism, Schizosaccharomyces genetics, Transcription, Genetic
Abstract

Reporter gene silencing assays provide a facile method for assessing the function of heterochromatin in Schizosaccharomyces pombe They use strains containing auxotrophic markers (commonly ura4 + or ade6 + ) located within a heterochromatic region. Transcriptional silencing of these reporters can be assessed by plating serial dilutions of cells onto minimal agar. In addition, silencing of ura4 + renders cells resistant to 5-fluoroorotic acid (5-FOA) and ade6 + silencing results in red colony color on adenine-limiting agar. Various reporters for each of the major heterochromatic domains (telomeres, centromeres, and the mating type locus) are available and, importantly, transcriptional silencing is correlated with the proper function of these regions., (© 2016 Cold Spring Harbor Laboratory Press.)

Published
2016
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13. A histone H3K36 chromatin switch coordinates DNA double-strand break repair pathway choice.

Author

Pai CC, Deegan RS, Subramanian L, Gal C, Sarkar S, Blaikley EJ, Walker C, Hulme L, Bernhard E, Codlin S, Bähler J, Allshire R, Whitehall S, and Humphrey TC

Subjects
Acetylation, Methylation, Schizosaccharomyces metabolism, Acetyltransferases metabolism, Chromatin metabolism, DNA End-Joining Repair, DNA Repair, DNA, Fungal metabolism, Histone-Lysine N-Methyltransferase metabolism, Histones metabolism, Recombinational DNA Repair, Schizosaccharomyces genetics, Schizosaccharomyces pombe Proteins metabolism
Abstract

DNA double-strand break (DSB) repair is a highly regulated process performed predominantly by non-homologous end joining (NHEJ) or homologous recombination (HR) pathways. How these pathways are coordinated in the context of chromatin is unclear. Here we uncover a role for histone H3K36 modification in regulating DSB repair pathway choice in fission yeast. We find Set2-dependent H3K36 methylation reduces chromatin accessibility, reduces resection and promotes NHEJ, while antagonistic Gcn5-dependent H3K36 acetylation increases chromatin accessibility, increases resection and promotes HR. Accordingly, loss of Set2 increases H3K36Ac, chromatin accessibility and resection, while Gcn5 loss results in the opposite phenotypes following DSB induction. Further, H3K36 modification is cell cycle regulated with Set2-dependent H3K36 methylation peaking in G1 when NHEJ occurs, while Gcn5-dependent H3K36 acetylation peaks in S/G2 when HR prevails. These findings support an H3K36 chromatin switch in regulating DSB repair pathway choice.

Published
2014
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14. Chromatin and the DNA damage response.

Author

Lydall D and Whitehall S

Subjects
Cell Cycle Proteins metabolism, DNA Repair, Histones chemistry, Humans, Intracellular Signaling Peptides and Proteins metabolism, Lysine metabolism, Methylation, Phosphoproteins metabolism, Telomere metabolism, Yeasts genetics, Yeasts metabolism, Chromatin metabolism, Chromatin Assembly and Disassembly, DNA Damage, Histones metabolism
Abstract

The impact of chromatin structure upon the DNA damage response is becoming increasingly apparent. We can reasonably expect many more papers showing how chromatin and chromatin modifications impact upon aspects of the DNA damage response. Here, we present our perspective on some recent developments in this exciting area of cell biology. We aim that this review will be of interest to those who study the DNA damage response, but not usually in the context of chromatin, and equally to those who study chromatin, but not the DNA damage response. It seems likely that these two communities will increasingly share common questions and interests.

Published
2005
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15. Transcriptional regulators of the Schizosaccharomyces pombe fbp1 gene include two redundant Tup1p-like corepressors and the CCAAT binding factor activation complex.

Author

Janoo RT, Neely LA, Braun BR, Whitehall SK, and Hoffman CS

Subjects
Amino Acid Sequence, Cloning, Molecular, Cyclic AMP-Dependent Protein Kinases metabolism, DNA, Complementary metabolism, Gene Deletion, Gene Library, Genotype, MAP Kinase Signaling System, Molecular Sequence Data, Mutation, Phenotype, Phosphorylation, Plasmids genetics, Promoter Regions, Genetic, Sequence Homology, Amino Acid, Signal Transduction, Temperature, Transcriptional Activation, beta-Galactosidase metabolism, CCAAT-Binding Factor genetics, Fructose-Bisphosphatase genetics, Fungal Proteins genetics, Gene Expression Regulation, Fungal, Nuclear Proteins, Repressor Proteins, Saccharomyces cerevisiae Proteins, Schizosaccharomyces genetics, Transcription, Genetic
Abstract

The Schizosaccharomyces pombe fbp1 gene, which encodes fructose-1,6-bis-phosphatase, is transcriptionally repressed by glucose through the activation of the cAMP-dependent protein kinase A (PKA) and transcriptionally activated by glucose starvation through the activation of a mitogen-activated protein kinase (MAPK). To identify transcriptional regulators acting downstream from or in parallel to PKA, we screened an adh-driven cDNA plasmid library for genes that increase fbp1 transcription in a strain with elevated PKA activity. Two such clones express amino-terminally truncated forms of the S. pombe tup12 protein that resembles the Saccharomyces cerevisiae Tup1p global corepressor. These clones appear to act as dominant negative alleles. Deletion of both tup12 and the closely related tup11 gene causes a 100-fold increase in fbp1-lacZ expression, indicating that tup11 and tup12 are redundant negative regulators of fbp1 transcription. In strains lacking tup11 and tup12, the atf1-pcr1 transcriptional activator continues to play a central role in fbp1-lacZ expression; however, spc1 MAPK phosphorylation of atf1 is no longer essential for its activation. We discuss possible models for the role of tup11- and tup12-mediated repression with respect to signaling from the MAPK and PKA pathways. A third clone identified in our screen expresses the php5 protein subunit of the CCAAT-binding factor (CBF). Deletion of php5 reduces fbp1 expression under both repressed and derepressed conditions. The CBF appears to act in parallel to atf1-pcr1, although it is unclear whether or not CBF activity is regulated by PKA.

Published
2001
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16. Microbial metallothioneins.

Author

Robinson NJ, Whitehall SK, and Cavet JS

Subjects
Amino Acid Sequence, Bacterial Proteins chemistry, Bacterial Proteins genetics, Bacterial Proteins metabolism, Cyanobacteria chemistry, Cyanobacteria genetics, Cyanobacteria metabolism, DNA-Binding Proteins chemistry, DNA-Binding Proteins genetics, DNA-Binding Proteins metabolism, DNA-Binding Proteins physiology, Gene Expression Regulation, Bacterial, Metallothionein chemistry, Metallothionein genetics, Metallothionein metabolism, Molecular Sequence Data, Mutation, Repressor Proteins chemistry, Repressor Proteins genetics, Repressor Proteins metabolism, Repressor Proteins physiology, Sequence Homology, Amino Acid, Zinc metabolism, Bacterial Proteins physiology, Cyanobacteria physiology, Metallothionein physiology
Abstract

Bacterial metallothioneins bind, sequester and buffer excess intracellular zinc. At present, the vast majority of the available experimental data relate to cyanobacterial metallothionein, SmtA, from Synechococcus PCC 7942. SmtA is required for normal resistance to zinc and smtA-mediated zinc resistance has been used as a selectable marker. The imidazole groups of histidine residues, in addition to the thiol groups of cysteine residues, co-ordinate zinc in bacterial metallothioneins. The structure of bacterial metallothionein must facilitate some discrimination between 'adventitious' and 'adventageous' zinc-binding sites such that under excess zinc conditions metal is predominantly scavenged from the former. It remains unclear whether or not bacterial metallothionein also acts as a zinc store that supplies zinc-requiring proteins or if under some conditions it deactivates a subset of proteins via zinc removal. Expression of smtA is induced in response to elevated concentrations of zinc via the action of SmtB. SmtB has some sequence similarity to the arsenic responsive repressor ArsR and genes encoding related proteins are present in many bacterial genomes. Metal perception by SmtB differs from ArsR. The latter contains a characteristic Cys-Val-Cys motif associated with a DNA-binding helix-turn-helix (the ArsR motif), while the former contains metal-binding motifs associated with a carboxyl-terminal alpha-helix that forms the interface between SmtB dimers (the SmtB motif). Some SmtB-ArsR family proteins, including the zinc sensor ZiaR from the cyanobacterium Synechocystis PCC 6803, have the metal-sensory motifs of both SmtB and ArsR. The mechanisms of action, and the features that allow discrimination between different metal ions by these sensors, are discussed.

Published
2001
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17. Cell cycle-regulated transcription in fission yeast: Cdc10-Res protein interactions during the cell cycle and domains required for regulated transcription.

Author

Whitehall S, Stacey P, Dawson K, and Jones N

Subjects
Cell Cycle Proteins genetics, DNA-Binding Proteins metabolism, Fungal Proteins metabolism, Gene Expression Regulation, Fungal, Mitosis genetics, Mutation, Protein Binding, Transcription Factors, Transcriptional Activation, Cell Cycle genetics, Cell Cycle Proteins metabolism, Schizosaccharomyces genetics, Schizosaccharomyces pombe Proteins, Transposases metabolism
Abstract

In Schizosaccharomyces pombe the MBF (DSC1) complex mediates transcriptional activation at Start and is composed of a common subunit called Cdc10 in combination with two alternative DNA-binding partners, Res1 and Res2. It has been suggested that a high-activity MBF complex (at G1/S) is switched to a low-activity complex (in G2) by the incorporation of the negative regulatory subunit Res2. We have analyzed MBF protein-protein interactions and find that both Res proteins are associated with Cdc10 throughout the cell cycle, arguing against this model. Furthermore we demonstrate that Res2 is capable of interacting with a mutant form of Cdc10 that has high transcriptional activity. It has been shown previously that both Res proteins are required for periodic cell cycle-regulated transcription. Therefore a series of Res1-Res2 hybrid molecules was used to determine the domains that are specifically required to regulate periodic transcription. In Res2 the nature of the C-terminal region is critical, and in both Res1 and Res2, a domain overlapping the N-terminal ankyrin repeat and a recently identified activation domain is important for mediating cell cycle-regulated transcription.

Published
1999
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18. Functional characterization of the fission yeast Start-specific transcription factor Res2.

Author

Zhu Y, Takeda T, Whitehall S, Peat N, and Jones N

Subjects
Cell Cycle Proteins metabolism, DNA-Binding Proteins metabolism, Dimerization, Electrophoresis, Polyacrylamide Gel, Fungal Proteins chemistry, GTP Phosphohydrolases, Gene Expression Regulation, Fungal, Meiosis, Membrane Proteins, Mutation genetics, Promoter Regions, Genetic genetics, Schizosaccharomyces cytology, Schizosaccharomyces genetics, Transcription Factors metabolism, Transcriptional Activation physiology, Transformation, Genetic genetics, DNA, Fungal metabolism, Fungal Proteins metabolism, Saccharomyces cerevisiae Proteins, Schizosaccharomyces metabolism, Schizosaccharomyces pombe Proteins
Abstract

In the fission yeast Schizosaccharomyces pombe, transcriptional activation at Start is mediated by complexes that bind the MCB. Two such complexes have been identified; both contain the Cdc10 protein in partnership with either the Res1 or Res2 protein. Characterization of null mutants suggests that the Res1-Cdc10 complex predominantly functions in mitotic cells whereas the Res2-Cdc10 complex is required for meiosis and spore formation. Here we have characterized the functional domains of the Res2 protein. The N-terminus is both necessary and sufficient for DNA binding, whereas the C-terminus is the region involved in the interaction with the Cdc10 protein. The centrally located ankyrin repeats are dispensable for both functions. Res2 binds to DNA as a dimer. In addition, complexes containing both Res1 and Res2 can form and bind to DNA in vitro. Furthermore, the major MCB-specific complex detected in extracts from wild-type cells contains Res1 and Res2; the complex is lost when either gene is deleted and can be recognized by antibodies specific to both proteins. In order to understand the basis for the specific function of Res2 in meiosis, hybrids between Res1 and Res2 were constructed and their functions analysed. The results indicate an absolute requirement for the Res2 C-terminus for normal meiosis to occur whereas the origin of the DNA-binding region is irrelevant. The implications of these results for the regulation of the MCB-binding complexes will be discussed.

Published
1997
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19. Yeast RNA polymerase III: transcription complexes and RNA synthesis.

Author

Geiduschek EP, Bardeleben C, Joazeiro CA, Kassavetis GA, and Whitehall S

Subjects
Animals, Promoter Regions, Genetic genetics, RNA Polymerase III genetics, RNA, Fungal biosynthesis, Ribonucleoprotein, U4-U6 Small Nuclear genetics, Saccharomyces cerevisiae genetics, Transcription Factors physiology
Abstract

In this article we review and summarize salient features of the mechanism of transcription by eukaryotic RNA polymerase III, focusing a considerable part of the account on the work of our laboratory, dealing with Saccharomyces cerevisiae, but emphasizing properties that are common to all eukaryotic RNA polymerases. The following topics are briefly discussed: promoter structure; transcription factors; internal structure of transcription complexes and DNA-protein interactions; U6 genes; promoter opening and RNA chain elongation; hydrolytic RNA retraction.

Published
1995

20. Hydrolytic cleavage of nascent RNA in RNA polymerase III ternary transcription complexes.

Author

Whitehall SK, Bardeleben C, and Kassavetis GA

Subjects
Adenosine Triphosphate metabolism, Cytidine Triphosphate metabolism, Dinucleoside Phosphates analysis, Dinucleoside Phosphates metabolism, Hydrolysis, Macromolecular Substances, Plasmids, RNA Polymerase III isolation & purification, RNA, Transfer, Tyr genetics, Templates, Genetic, RNA metabolism, RNA Polymerase III metabolism, Ribonucleases metabolism, Saccharomyces cerevisiae enzymology, Transcription, Genetic
Abstract

Highly purified yeast RNA polymerase III ternary complexes were found to possess a hydrolytic chain retracting activity that cleaves nascent RNA from its 3'-OH end. Most of the shortened transcripts were capable of resuming RNA chain elongation, indicating that they remain stably associated with the enzyme-DNA complex. Analysis of the products of cleavage indicated that retraction primarily occurred in dinucleotide increments, but that mononucleotides were also excised at lower frequency. The ribonuclease activity was totally dependent on the presence of a divalent cation and was stimulated by the addition of non-cognate ribonucleotides. The inclusion of ATP in the reaction enhanced both the rate and extent of transcript cleavage. Evidence suggesting that the hydrolytic activity is intrinsic to RNA polymerase III and factor-independent is also presented. Transcript cleavage by RNA polymerase III ternary complexes appears to be more closely related to the intrinsic nucleolytic activity of vaccinia virus RNA polymerase ternary complexes than to TFIIS-dependent cleavage that has been described for RNA polymerase II ternary complexes.

Published
1994

21. The function of the upstream region of the sigma 54-dependent Klebsiella pneumoniae nifL promoter is sensitive to DNA supercoiling.

Author

Whitehall S, Austin S, and Dixon R

Subjects
Bacterial Proteins biosynthesis, DNA, Bacterial chemistry, DNA, Superhelical chemistry, DNA-Directed RNA Polymerases metabolism, Enhancer Elements, Genetic, Escherichia coli genetics, Escherichia coli metabolism, Kinetics, Klebsiella pneumoniae genetics, Nitrogen Fixation genetics, Nucleic Acid Conformation, Plasmids, Potassium Permanganate, Transcription, Genetic, beta-Galactosidase analysis, beta-Galactosidase biosynthesis, Bacterial Proteins genetics, DNA, Bacterial metabolism, DNA, Superhelical metabolism, Genes, Bacterial, Klebsiella pneumoniae metabolism, Promoter Regions, Genetic genetics, Sigma Factor metabolism
Abstract

The positive control protein NTRC activates transcription from the sigma 54-dependent nifL and glnAp2 promoters of Klebsiella pneumoniae by binding to upstream enhancer-like sequences and contacting downstream bound sigma 54-RNA polymerase via looping of the intervening DNA. In contrast to the glnAp2 promoter, the activity of the nifL promoter is very sensitive to changes in DNA supercoiling both in vivo and in vitro. We have shown previously that the downstream elements of the nifL promoter are involved in the supercoiling response. In this study we find that the upstream region of nifL influences the supercoiling response of a hybrid nifL-glnAp2 promoter both in vivo and in vitro, demonstrating that the nifL upstream region also confers supercoiling sensitivity. DNA supercoiling did not appear to influence binding of NTRC to its sites in the nifL upstream region, suggesting that another function of this region, most probably DNA loop formation, is sensitive to changes in DNA topology.

Published
1993
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22. DNA supercoiling response of the sigma 54-dependent Klebsiella pneumoniae nifL promoter in vitro.

Author

Whitehall S, Austin S, and Dixon R

Subjects
Base Sequence, DNA-Binding Proteins metabolism, DNA-Directed RNA Polymerases metabolism, In Vitro Techniques, Macromolecular Substances, Molecular Sequence Data, Mutagenesis, Site-Directed, Transcription, Genetic, DNA, Bacterial chemistry, DNA, Superhelical, Genes, Bacterial, Klebsiella pneumoniae genetics, Nitrogen Fixation genetics, Promoter Regions, Genetic, Sigma Factor genetics
Abstract

Transcription from the sigma 54-dependent Klebsiella pneumoniae nifL and glnAp2 promoters is activated by the general nitrogen regulatory protein NTRC. Unlike the glnAp2 promoter, which is relatively insensitive to changes in DNA supercoiling, transcription from nifL in vitro in a chloride-based buffer is supercoiling-dependent at physiological salt concentrations. The replacement of chloride with an acetate-based buffer decreases the stringency of the nifL supercoiling response, but open complexes formed on linear nifL promoter DNA under these conditions are unstable and less extensive than those found on supercoiled (form I) DNA. We have introduced mutations in particular elements of the nifL promoter that increase its homology to glnAp2. At the wild-type nifL promoter, sigma 54-RNA polymerase makes only limited contacts with the promoter in the absence of NTRC. However, a G to T change at -26 (nifL74) allows the formation of a stable closed complex with sigma 54-holoenzyme on both linear and form I templates in the absence of the activator. The combination of C to T mutations at -3 and -1 (nifL18) increases the A+T rich nature of the melted region and stabilizes open complexes formed on linear DNA. Open complex formation as a function of superhelical density was assessed at each promoter. Formation of open complexes at glnAp2 peaks at -0.024 and declines at higher superhelical densities, whereas at the wild-type nifL promoter, open complex formation peaks at -0.067 and is not detectable at superhelical densities less than -0.032. Both the nifL74 and nifL18 mutations altered the supercoiling response, increasing the ability to form open complexes at low superhelical densities. The presence of the nifL74 and nifL18 mutations in combination further altered the response of the promoter to DNA supercoiling. These observations suggest that the promoter as a whole, and not any one promoter element, mediates the transcriptional response to DNA supercoiling.

Published
1992
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