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Jonathan Richard White,1 Jody Anne Winter,2 Karen Robinson1 1NIHR Biomedical Research Unit in Gastrointestinal and Liver Diseases at Nottingham University Hospitals NHS Trust and The University of Nottingham, Nottingham, 2Interdisciplinary Biomedical Research Centre, School of Science and Technology, Nottingham Trent University, Nottingham, UK Abstract: The bacterial pathogen Helicobacter pylori commonly colonizes the human gastric mucosa during early childhood and persists throughout life. The organism has evolved multiple mechanisms for evading clearance by the immune system and, despite inducing inflammation in the stomach, the majority of infections are asymptomatic. H. pylori is the leading cause of peptic ulcer disease and gastric cancer. However, disease outcomes are related to the pattern and severity of chronic inflammation in the gastric mucosa, which in turn is influenced by both bacterial and host factors. Despite over 2 decades of intensive research, there remains an incomplete understanding of the circumstances leading to disease development, due to the fascinating complexity of the host–pathogen interactions. There is accumulating data concerning the virulence factors associated with increased risk of disease, and the majority of these have pro-inflammatory activities. Despite this, only a small proportion of those infected with virulent strains develop disease. Several H. pylori virulence factors have multiple effects on different cell types, including the induction of pro- and anti-inflammatory, immune stimulatory, and immune modulatory responses. The expression of multiple virulence factors is also often linked, making it difficult to assess the meaning of their effects in isolation. Overall, H. pylori is thought to usually modulate inflammation and limit acute damage to the mucosa, enabling the bacteria to persist. If this delicate balance is disturbed, disease may then develop. Keywords: Helicobacter pylori, inflammation, mucosal immunity, peptic ulcer disease, gastric cancer

Variety development of sugarcane (Saccharum spp. hybrids) is necessary to continue improving sugar yields and selecting photosynthetic traits can improve sugar production through increased carbon inputs. In this study, gas exchange and whole-plant measurements were made on 55 sugarcane genotypes in Louisiana. Variation in the relationship between photosynthetic rate and stomatal conductance suggests that sugarcane exhibits variation in both photosynthetic capacity and CO2 substrate availability. Genotypes that reached maximum photosynthetic rate (TRMPR) in the gas-exchange cuvette more quickly had greater CO2 assimilation during transitory periods. Temporary shading and fluctuating light are common transitory conditions in the field, so increasing TRMPR can improve photosynthesis in water-abundant regions. Canopy leaf area was positively correlated with stalk mass, but gas-exchange traits were not correlated with whole-plant traits. A better understanding of the relationship between leaf and whole-plant traits is necessary to identify physiological traits that lead to increased genetic gain.

Drought is a prolonged dry period that can have severe impacts on the environment, human health, economies, agriculture, and energy resources. It can lead to water shortages, ruin crops, dry out forests, and reduce the availability of food and water for wildlife and livestock. The primary objectives of this study are to: 1) quantify the variability and distributions of drought patterns in Arkansas, United States (US), 2) use remotely sensed indices to investigate the correlation between drought and vegetation cover in the area, and 3) develop a cloud-based framework (user-friendly app) to facilitate the assessment of drought impact in Arkansas over the past decades. A correlation analysis was also performed between the Vegetation Health Index (VHI) and meteorological indices to better understand the impact of meteorological drought on vegetation stress. In addition, Mann-Kendall trend analysis was used to assess trends in meteorological drought indices. The results indicate that drought is most prevalent during March and August months. The results of this study revealed that approximately 31% of the study area fell under the four drought classes (i.e., 1% Extreme drought, 4% Severe drought, 9% moderate drought, and 19% mild drought), with spring and the growing season experiencing moderate drought, particularly in agricultural areas, most notably within the Mississippi Alluvial Valley Plain at both state and county levels. In August, approximately 31% of the study area fell under the Four drought classes (i.e., 1% Extreme drought, 4% Severe drought, 9% moderate drought, and 19% mild drought), with spring and the growing season experiencing moderate drought, particularly in agricultural areas, most notably within the Mississippi Alluvial Valley Plain at both state and county levels. This study provides an essential foundation for policymakers, environmental scientists, and agricultural stakeholders aiming to mitigate drought impacts and safeguard against future climate uncertainties.

Abstract Sugarcane (Saccharum spp.) represents the most valuable row crop in Louisiana. High levels of biomass production and extensive tillage have degraded portions of the state's alluvial soils used to grow sugarcane. In addition to sucrose, processing the crop generates excess bagasse each year, which can represent a disposal problem for sugar factories. However, converting the bagasse to biochar at nearby pyrolysis facilities may prove to be an economical means of improving degraded soils. The objective was to determine the impacts of low rates of biochar (86%. Biochar did not increase soil CO2 evolution, indicating its stability as a soil carbon amendment. However, adding biochar with mineral nitrogen decreased CO2 evolution, compared to biochar alone, indicating a negative priming effect. Soil moisture retention was minimally impacted by biochar. Cane yield, sucrose content, and sucrose yield were not statistically affected by applying biochar with or without starter fertilizer at planting. Overall, the results indicate that lower levels of bagasse‐derived biochar minimally impacted soil properties and crop yield; however, the biochar was stable in soil and may find utility as a carbon‐rich amendment should carbon credits prove to be an additional source of grower or land‐owner revenue.

Deer hunter surveys commonly employ hypothetical chronic wasting disease (CWD) prevalence scenarios to measure behavioral intentions. These surveys may suffer from hypothetical bias (i.e. respondents describe unrealistic behaviors in hypothetical situations) and it is unclear how hypothetical bias affects results. We used an online-based survey of Arkansas deer hunters to (1) investigate how behavioral intentions change as CWD prevalence hypothetically varies, and (2) explore the presence of hypothetical bias in behavioral intention by comparing responses across hypothetical prevalence rates from those whose primary hunting counties had CWD detected versus not. We found undesired behaviors (e.g. hunting less often, hunting cessation) were unlikely to change substantially as prevalence increased, and assistance with some CWD monitoring and management behaviors may increase. We found some evidence of hypothetical bias in anticipated behavior change. Agencies should be aware that survey respondents may overstate behavioral intentions, and should consider this when making CWD management decisions. [ABSTRACT FROM AUTHOR]

Fasciola hepatica has a complex lifecycle with multiple intermediate and definitive hosts and influenced by environmental factors. The disease causes significant morbidity in children and its prevalent worldwide. There is lack of data about distribution and burden of the disease in endemic regions, owing to poor efficacy of the different diagnostic methods used. A novel PCR-based test was developed by using a portable mini-PCR® platform to detect Fasciola sp. DNA and interpret the results via a fluorescence viewer and smartphone image analyzer application. Human stool, snail tissue, and water samples were used to extract DNA. Primers targeting the ITS-1 of the 18S rDNA gene of Fasciola sp. were used. The limit of detection of the mini-PCR test was 1 fg/μL for DNA samples diluted in water, 10 fg/μL for Fasciola/snail DNA scramble, and 100 fg/μL for Fasciola/stool DNA scramble. The product detection by agarose gel, direct visualization, and image analyses showed the same sensitivity. The Fh mini-PCR had a sensitivity and specificity equivalent to real-time PCR using the same specimens. Testing was also done on infected human stool and snail tissue successfully. These experiments demonstrated that Fh mini-PCR is as sensitive and specific as real time PCR but without the use of expensive equipment and laboratory facilities. Further testing of multiple specimens with natural infection will provide evidence for feasibility of deployment to resource constrained laboratories. [ABSTRACT FROM AUTHOR]

Variety development of sugarcane (Saccharum spp. hybrids) is necessary to continue improving sugar yields and selecting photosynthetic traits can improve sugar production through increased carbon inputs. In this study, gas exchange and whole-plant measurements were made on 55 sugarcane genotypes in Louisiana. Variation in the relationship between photosynthetic rate and stomatal conductance suggests that sugarcane exhibits variation in both photosynthetic capacity and CO2 substrate availability. Genotypes that reached maximum photosynthetic rate (TRMPR) in the gas-exchange cuvette more quickly had greater CO2 assimilation during transitory periods. Temporary shading and fluctuating light are common transitory conditions in the field, so increasing TRMPR can improve photosynthesis in water-abundant regions. Canopy leaf area was positively correlated with stalk mass, but gas-exchange traits were not correlated with whole-plant traits. A better understanding of the relationship between leaf and whole-plant traits is necessary to identify physiological traits that lead to increased genetic gain. [ABSTRACT FROM AUTHOR]

In this autoethnographic essay, the author explores a wide range of personal experiences as a neurodivergent student. The author challenges the reader (students, educators, parents, whoever they may be) to self-reflect on their own experiences with the neurodivergent community to broaden the understanding of each person's unique academic journey. The author's hope is that readers feel inspired to recognize their own personal capacity to create real change in expanding upon what it means to be part of the neurodivergent community and beyond. [ABSTRACT FROM AUTHOR]

The caffeine metabolic ratio is an established marker for cytochrome P450 (CYP) 1A2 activity. Optimal sample size calculation for clinical pharmacokinetic xenobiotic–caffeine interaction studies requires robust estimates of interindividual and intraindividual variation in this ratio. Compared with interindividual variation, factors contributing to intraindividual variation are less defined. An exploratory analysis involving healthy nonsmoking non‐naïve caffeine drinkers (1–3 cups/day; 12 men, 12 women) administered caffeine (160 mg) on five occasions evaluated the effects of CYP1A2 induction status (based on genotype) and other factors on intraindividual variation in CYP1A2 activity. Results were compared with those from previous studies. Regardless of whether a hyperinducer (CYP1A2*1A/*1F or CYP1A2*1F/*1F) or normal metabolizer (CYP1A2*1A/*1A, CYP1A2*1C/*1F, or CYP1A2*1C*1F/*1C*1F), sex, age, oral contraceptive use by women, and smoking status, intraindividual variation was ≤30%. A value of 30% is proposed for optimal design of pharmacokinetic xenobiotic–caffeine interaction studies. Prospective studies are needed for confirmation.

We describe efforts to reintroduce the extinct-in-the-wild Spix's Macaw (Cyanopsitta spixii) within its historical range in eastern Brazil. Twenty captive-reared Spix's Macaws were released, along with 15 Blue-Winged Macaws (Primolius maracana), as heterospecific flocks in two events during the dry and rainy seasons of 2022. We monitored the release groups through daily observation and telemetry tracking from early June 2022 to early June 2023. We documented an overall first-year survival of 58.3% (CI: 37.8–78.7%), with 65% of Spix's Macaws establishing a stable area of activity within 5 km of the release site, excluding any temporary long-distance forays. Eighty-five percent of released Spix's Macaws exhibited flock cohesion, including interactions with and integration into wild Blue-Winged Macaw groups at the release site. Several released Spix's Macaws formed pair-bonds with conspecifics and engaged in nest cavity exploration, breeding behavior, and territorial defense of nest sites, with three females also laying and incubating eggs. One nesting pair successfully hatched and reared chicks in an artificial nest cavity. These releases employed a novel reintroduction strategy using a surrogate species model, the sympatric Blue-Winged Macaw, to "mentor" and facilitate post-release adaptation by the target species and increase the total number of individuals in the release cohort. Lastly, participatory monitoring by local citizens is considered a way to engage and involve local communities in species and habitat conservation and potentially create new employment opportunities in the region. [ABSTRACT FROM AUTHOR]

When Jimmy Carter became the U.S. president in January 1977, the United States and Oman did not have any formal security arrangements, apart from occasional landings of U.S. military aircraft on Oman's Masirah Island. Although Oman's leader, Sultan Qaboos bin Said, hoped to establish closer security ties with the United States, the Carter administration chose to continue President Richard Nixon's policy of relying on the "twin pillars" of Iran and Saudi Arabia to reinforce Western security in the Persian/Arabian Gulf region. Oman remained mostly a strategic afterthought until a series of crises unfolded in the greater Middle East: a Communist coup in Afghanistan in April 1978, the fall of the Shah of Iran in January 1979, and a war between Marxist South Yemen and North Yemen shortly thereafter. In November 1979, Iranian terrorists backed by the Islamic regime stormed the U.S. embassy in Tehran and seized dozens of hostages. This was followed a month later by the Soviet invasion of Afghanistan. With the fall of the Shah and the collapse of the "twin pillars" policy, the Carter administration began a sustained effort to create a Rapid Deployment Force for the Middle East and to gain access to Oman's strategic military facilities. After several months of arduous negotiations, Carter and his team were able to conclude a Facilities Access Agreement with Oman in June 1980, a transformative process that gave Washington the ability to insert U.S. military personnel into the Gulf and Indian Ocean on a contingency basis. The Carter administration's effort stands as a significant achievement, converting Oman into the most reliable U.S. ally in the Gulf region in the 1980s. [ABSTRACT FROM AUTHOR]

Sugarcane croplands account for ~70% of global sugar production and ~60% of global ethanol production. Monitoring and predicting gross primary production (GPP) and transpiration (T) in these fields is crucial to improve crop yield estimation and management. While moderate-spatial-resolution (MSR, hundreds of meters) satellite images have been employed in several models to estimate GPP and T, the potential of high-spatial-resolution (HSR, tens of meters) imagery has been considered in only a few publications, and it is underexplored in sugarcane fields. Our study evaluated the efficacy of MSR and HSR satellite images in predicting daily GPP and T for sugarcane plantations at two sites equipped with eddy flux towers: Louisiana, USA (subtropical climate) and Sao Paulo, Brazil (tropical climate). We employed the Vegetation Photosynthesis Model (VPM) and Vegetation Transpiration Model (VTM) with C4 photosynthesis pathway, integrating vegetation index data derived from satellite images and on-ground weather data, to calculate daily GPP and T. The seasonal dynamics of vegetation indices from both MSR images (MODIS sensor, 500 m) and HSR images (Landsat, 30 m; Sentinel-2, 10 m) tracked well with the GPP seasonality from the EC flux towers. The enhanced vegetation index (EVI) from the HSR images had a stronger correlation with the tower-based GPP. Our findings underscored the potential of HSR imagery for estimating GPP and T in smaller sugarcane plantations. [ABSTRACT FROM AUTHOR]

Due to complex polyploid, sugarcane whole genome sequencing and characterization lag far behind other crops. PCR-based DNA markers are a viable low-cost option to evaluate genetic diversity and verify genotypes. In this study, the 5S ribosomal RNA-intergenic spacer (ITS) of 171 accessions of Saccharum spp. and Tripidium spp. was dissected, including 30 accessions of S. officinarum, 71 of S. spontaneum, 17 of S. robustum, 25 of S. barberi, 13 of S. sinense, 2 of S. edule, 5 sugarcane cultivars (Saccharum spp. hybrids), 6 of Tripidium spp. (formally Erianthus spp.), and 2 of unknown species. The ITS spacers were amplified from 10 ng of the leaf DNA of each accession with the universal PCR primers PI and PII. The PCR-amplified spacers (amplicons) were analyzed by both agarose gel and capillary electrophoresis (CE). While agarose gel electrophoresis revealed five banding patterns, a total of 42 polymorphic amplicons, ranging from 60 to 506 bp, were detected by CE. Three amplicons, 234-, 235-, and 236-bp in size, were amplified from all accessions of six Saccharum species, except for three S. robustum accessions (Molokai 5573, NG 57-054, and NG 77-235) that lacked the 236-bp amplicon. The 234-, 235-, 236-bp banding pattern found in S. spontaneum was less consistent than other Saccharum species, sometimes missing a few but not all the bands in this region. An amplicon of 61-bp was amplified only from the sugarcane hybrid varieties. The PI/PII patterns indicated diversity and subpopulations within Saccharum, which could potentially be used in Breeding. Moreover, all Saccharum-specific amplicons were mostly absent in Tripidium spp. accessions, which produced 405-bp and 406-bp amplicons, and any pattern of the exceptions indicated misidentification. The T. bengalense accession Kalimpong had a unique CE-banding pattern that was different from all other accessions. Although the clustering pattern of the 42 amplicons only discriminated at the genus level, these amplicons helped identify nine misclassified accessions. This study further demonstrates that these PI/PII amplicons could be particularly useful markers for breeders at sugarcane field stations to quickly confirm and discriminate among the accessions of germplasm collections. [ABSTRACT FROM AUTHOR]