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Abstract Background Plasmodium knowlesi is an established experimental model for basic and pre-clinical malaria vaccine research. Historically, rhesus macaques have been the most common host for malaria vaccine studies with P. knowlesi parasites. However, rhesus are not natural hosts for P. knowlesi, and there is interest in identifying alternative hosts for vaccine research. The study team previously reported that pig-tailed macaques (PTM), a natural host for P. knowlesi, could be challenged with cryopreserved P. knowlesi sporozoites (PkSPZ), with time to blood stage infection equivalent to in rhesus. Here, additional exploratory studies were performed to evaluate PTM as potential hosts for malaria vaccine studies. The aim was to further characterize the parasitological and veterinary health outcomes after PkSPZ challenge in this macaque species. Methods Malaria-naïve PTM were intravenously challenged with 2.5 × 103 PkSPZ and monitored for blood stage infection by Plasmodium 18S rRNA RT-PCR and thin blood smears. Disease signs were evaluated by daily observations, complete blood counts, serum chemistry tests, and veterinary examinations. After anti-malarial drug treatment, a subset of animals was re-challenged and monitored as above. Whole blood gene expression analysis was performed on selected animals to assess host response to infection. Results In naïve animals, the kinetics of P. knowlesi blood stage replication was reproducible, with parasite burden rising linearly during an initial acute phase of infection from 6 to 11 days post-challenge, before plateauing and transitioning into a chronic low-grade infection. After re-challenge, infections were again reproducible, but with lower blood stage parasite densities. Clinical signs of disease were absent or mild and anti-malarial treatment was not needed until the pre-defined study day. Whole blood gene expression analysis identified immunological changes associated with acute and chronic phases of infection, and further differences between initial challenge versus re-challenge. Conclusions The ability to challenge PTM with PkSPZ and achieve reliable blood stage infections indicate this model has significant potential for malaria vaccine studies. Blood stage P. knowlesi infection in PTM is characterized by low parasite burdens and a benign disease course, in contrast with the virulent P. knowlesi disease course commonly reported in rhesus macaques. These findings identify new opportunities for malaria vaccine research using this natural host-parasite combination. Graphical Abstract

OBJECTIVES/GOALS: This work aims to explore how citizen science serves as a transformative frame work to bridge scientific knowledge, focusing on its potential to enhance transdisciplinary learning in artificial intelligence (AI) biomedical and clinical sciences by facilitating near-peer mentoring. METHODS/STUDY POPULATION: Our group of eight friends comprise a multicultural and multidisciplinary cohort including students from the USA, Philippines, Indonesia, and Guatemala pursuing PhD degrees in electrical and computer engineering, epidemiology, physics, and MD, PharmD, and DMD degrees. We engage in shared online courses, collaborative projects, and abstract submissions. Employing our collective knowledge, we design interactive learning experiences, support each other’s initiatives, and collaboratively develop lectures and presentations. We in tend to expand collaborations in biomedical AI education while fostering principles of experiential and collaborativelearning, constructivism, and authentic inquiry. RESULTS/ANTICIPATED RESULTS: Our recent successes include submittedconference abstracts on data science and AI education in pharmacy and the facilitation of a guest lecture in health informatics. Additionally, we are currently collaborating on seven biomedical machine learning projects in radio frequency engineering, aiming for conference submissions. Moving forward, our goal is to expand our group, support the formation of similar communities, and promote data science and AI literacy in biomedical and clinical contexts. We aspire to extend this knowledge to families, classmates, and eventually patients, facilitating a broader understanding of the role of AI in healthcare. DISCUSSION/SIGNIFICANCE: We believe diverse expertise and pedagogical theories can help demonstrate the potential of citizen science to democratize scientific experience. By nurturing collaborative networks our efforts aim to bridge gaps between disciplines and enhance the broader public’s understanding of AI in healthcare.

Abstract Heritable color polymorphisms have a long history of study in evolutionary biology, though they are less frequently examined today than in the past. These systems, where multiple discrete, visually identifiable color phenotypes co‐occur in the same population, are valuable for tracking evolutionary change and ascertaining the relative importance of different evolutionary mechanisms. Here, we use a combination of citizen science data and field surveys in the Great Lakes region of North America to identify patterns of color morph frequencies in the eastern gray squirrel (Sciurus carolinensis). Using over 68,000 individual squirrel records from both large and small spatial scales, we identify the following patterns: (a) the melanistic (black) phenotype is often localized but nonetheless widespread throughout the Great Lakes region, occurring in all states and provinces sampled. (b) In Ohio, where intensive surveys were performed, there is a weak but significantly positive association between color morph frequency and geographic proximity of populations. Nonetheless, even nearby populations often had radically different frequencies of the melanistic morph, which ranged from 0% to 96%. These patterns were mosaic rather than clinal. (c) In the Wooster, Ohio population, which had over eight years of continuous data on color morph frequency representing nearly 40,000 records, we found that the frequency of the melanistic morph increased gradually over time on some survey routes but decreased or did not change over time on others. These differences were statistically significant and occurred at very small spatial scales (on the order of hundreds of meters). Together, these patterns are suggestive of genetic drift as an important mechanism of evolutionary change in this system. We argue that studies of color polymorphism are still quite valuable in advancing our understanding of fundamental evolutionary processes, especially when coupled with the growing availability of data from citizen science efforts.

Intraoperative electrocochleography (ECOG) is performed using a single low-frequency acoustic stimulus (e.g., 500 Hz) to monitor cochlear microphonics (CM) during cochlear implant (CI) electrode insertion. A decrease in CM amplitude is commonly associated with cochlear trauma and is used to guide electrode placement. However, advancement of the recording electrode beyond the sites of CM generation can also lead to a decrease in CM amplitude and is sometimes interpreted as cochlear trauma, resulting in unnecessary electrode manipulation and increased risk of cochlear trauma during CI electrode placement. In the present study, multi-frequency ECOG was used to monitor CM during CI electrode placement. The intraoperative CM tracings were compared with electrode scan measurements, where CM was measured for each of the intracochlear electrodes. Comparison between the peak CM amplitude measured during electrode placement and electrode scan measurements was used to differentiate between different mechanisms for decrease in CM amplitude during CI electrode insertion. Analysis of the data shows that both multi-frequency electrocochleography and electrode scan could potentially be used to differentiate between different mechanisms for decreasing CM amplitude and providing appropriate feedback to the surgeon during CI electrode placement.

Until recently, chirality has not been a major focus in the study of cannabinoids, as most cannabinoids of interest, such as cannabidiol and tetrahydrocannabinol, exist as a single isomer from natural sources. However, this is changing as more cannabinoids are identified, and compounds such as cannabichromene and cannabicyclol are emerging as potential investigatory candidates for varying indications. Because these molecules are chiral, the separation and study of the individual enantiomers’ biological and physiological effects should therefore be of interest. The purpose of this study was to identify analytical separation conditions and then adapt those conditions to preparative separation. This was accomplished with a column-screening approach on Daicel’s immobilized polysaccharide chiral stationary phases using non-traditional mobile phases, which included dichloromethane, ethyl acetate, and methyl tert-butyl ether under high-performance liquid chromatography conditions. CHIRALPAK® IK was found to separate all four compounds well with mobile phases containing hexane-dichloromethane (with or without an acidic additive). From these methods, the separation productivities were calculated to better visualize the separation scalability, which shows that the kilogram-scale separations of each are feasible.

The increased use and applicability of Cannabis and Cannabis-derived products has skyrocketed over the last 5 years. With more and more governing bodies moving toward medical and recreational legalization, the need for robust and reliable analytical testing methods is also growing. While many stationary phases and methods have been developed for this sort of analysis, chiral stationary phases (CSPs) are unique in this area; not only can they serve their traditional chiral separation role, but they can also be used to perform achiral separations. Given that mixtures of cannabinoids routinely contain enantiomers, diastereomers, and structural isomers, this offers an advantage over the strictly achiral-only analyses. This work presents the separation of a 10-cannabinoid mixture on several polysaccharide-based sub-2 µm CSPs with both normal-phase and reversed-phase ultra-high-performance liquid chromatography (UHPLC) conditions. Along with the separation of the mixture, appropriate single-peak identification was performed to determine the elution order and reported where applicable.

Urban systems often support large numbers of non-native species, but due to the heterogeneity of urban landscapes, species are not evenly distributed. Understanding the drivers of ecological resistance in urban landscapes may help to identify habitats that are most resistant to invasion, and inform efforts to model and conserve native biodiversity. We used pitfall traps to survey non-native ground-dwelling arthropods in three adjacent, low-elevation habitat types in southern California: California sage scrub, non-native grassland, and suburban development. We found that non-native species were fewer and less widely distributed in the sage scrub and grassland habitats. Due to the proximity of our sites, differences in propagule pressure is an unlikely explanation. Instead, we suggest that the absence of water subsidies in the sage scrub and grassland habitats increases those habitats’ resistance to arthropod invasions. Comparisons to studies conducted at fragments closer to the coast provide further support for the relationship between aridity and invasibility in southern California. Our findings highlight that inland fragments are important for conserving native arthropod diversity, that models of non-native species distributions in arid and semi-arid urban systems should include aridity measures, and that reducing resource subsidies across the region is critical to mitigating spread of non-natives.

We conducted a preregistered multilaboratory project (k = 36; N = 3,531) to assess the size and robustness of ego-depletion effects using a novel replication method, termed the paradigmatic replication approach. Each laboratory implemented one of two procedures that was intended to manipulate self-control and tested performance on a subsequent measure of self-control. Confirmatory tests found a nonsignificant result (d = 0.06). Confirmatory Bayesian meta-analyses using an informed-prior hypothesis (δ = 0.30, SD = 0.15) found that the data were 4 times more likely under the null than the alternative hypothesis. Hence, preregistered analyses did not find evidence for a depletion effect. Exploratory analyses on the full sample (i.e., ignoring exclusion criteria) found a statistically significant effect (d = 0.08); Bayesian analyses showed that the data were about equally likely under the null and informed-prior hypotheses. Exploratory moderator tests suggested that the depletion effect was larger for participants who reported more fatigue but was not moderated by trait self-control, willpower beliefs, or action orientation.

The marriage of Aboriginal perspectives and mathematics is complex and comes with multiple interpretations. Through the research presented in this paper, we propose that one possibility for a lasting relationship between Aboriginal perspectives and mathematics lies in understanding more about teachers' experiences and stories from their own mathematics classrooms, with their own students. The purpose of this paper, and of the research project informing this paper, is to understand how Grade 6 teachers in one particular Canadian province (Saskatchewan) are addressing Aboriginal-focused curriculum goals/outcomes and to listen to teachers' perspectives on teaching mathematics with a distinctly Aboriginal focus. Data collection consisted of focus group discussions, individual interviews, and classroom observations with three case study teachers (Chris, Joe, and Lindsay). In this paper, we present three brief vignettes constructed out of the data, which provide a glimpse into the uniqueness of each teacher, each classroom, and each interpretation of what it means to teach mathematics through a distinctly Aboriginal focus.

Since its inception in 1990, Tyler's process-based model of regulation has provided a fundamental framework for understanding why people obey the law. Numerous studies have found support for Tyler's process-based model of regulation, with the majority of research reporting an association between procedural justice and legitimacy. The research is limited, however, in its ability to explain the correlates of legitimacy outside of procedural justice, which is surprising considering research indicates that the development of behaviors and attitudes toward legal institutions and actors is complex. Based on the legal socialization literature, the current study explores how perceptions of police legitimacy may be impacted by family and/or peer views of the police. In order to investigate this relationship, the current exploratory study relies on data from students located at two universities in the United States. The results suggest that family views, peer views, and procedural justice are all significantly related to perceived police legitimacy. These findings are explored and contextualized in the broader literature on police legitimacy. [ABSTRACT FROM AUTHOR]

A joint earthquake source inversion technique is presented that uses InSAR and long-period teleseismic data, and, for the first time, takes 3-D Earth structure into account when modelling seismic surface and body waves. Ten average source parameters (Moment, latitude, longitude, depth, strike, dip, rake, length, width and slip) are estimated; hence, the technique is potentially useful for rapid source inversions of moderate magnitude earthquakes using multiple data sets. Unwrapped interferograms and long-period seismic data are jointly inverted for the location, fault geometry and seismic moment, using a hybrid downhill Powell-Monte Carlo algorithm. While the InSAR data are modelled assuming a rectangular dislocation in a homogeneous halfspace, seismic data are modelled using the spectral element method for a 3-D earth model. The effect of noise and lateral heterogeneity on the inversions is investigated by carrying out realistic synthetic tests for various earthquakes with different faulting mechanisms and magnitude (Mw 6.0-6.6). Synthetic tests highlight the improvement in the constraint of fault geometry (strike, dip and rake) and moment when InSAR and seismic data are combined. Tests comparing the effect of using a 1-D or 3-D earth model show that long-period surface waves are more sensitive than long-period body waves to the change in earth model. Incorrect source parameters, particularly incorrect fault dip angles, can compensate for systematic errors in the assumed Earth structure, leading to an acceptable data fit despite large discrepancies in source parameters. Three real earthquakes are also investigated: Eureka Valley, California (1993 May 17, Mw 6.0), Aiquile, Bolivia (1998 February 22, Mw 6.6) and Zarand, Iran (2005 May 22, Mw 6.5). These events are located in different tectonic environments and show large discrepancies between InSAR and seismically determined source models. Despite the 40-50 km discrepancies in location between previous geodetic and seismic estimates for the Eureka Valley and Aiquile earthquakes, the seismic data are found to be compatible with the InSAR location. A 30° difference in strike between InSAR and seismic-derived source models is also resolved when taking 3-D Earth structure into account in the analysis of the Eureka Valley earthquake. The combination of both InSAR and seismic data further constrains the dip for the Zarand earthquake, and in all cases the seismic moment is more robustly constrained in the joint inversions than in the individual data set inversions. Unmodelled lateral heterogeneities in Earth and the models could partly explain some of the observed source parameter discrepancies related to the seismic data. © The Authors 2014. Published by Oxford University Press on behalf of The Royal Astronomical Society.

An optimized method employing chiral supercritical fluid chromatography with diode array UV–VIS detection has been developed for the quantitative analysis of nicotine and nornicotine enantiomer distributions. The method parameters that were optimized included: column type (stationary phases, Chiralpak IG-3), column temperature (40°C), modifier types and concentration (isopropyl alcohol, 10%), additive types and concentrations (diethylamine, 0.2%), elution times (<6 min, flow rate 3 mL/min) and resolution factor (>1.2). These optimized conditions led to nicotine and nornicotine enantiomer detection limits of ~5 ng/μL with accompanying %RSD values of <2% from the analyses of commercially available nicotine-containing formulations. [ABSTRACT FROM AUTHOR]

Drawing from organizational justice theory, this study examined the mediating effects of organizational trust on the association between organizational justice (i.e. in the forms of distributive and procedural justice) and the job attitudes of job satisfaction and organizational commitment in a sample of 220 correctional staff employed full-time at a high-security prison in the U.S. Midwest. Ordinary least squares regression results indicated that participant perceptions of organizational trust fully mediated the association between procedural justice and job satisfaction, but only partially mediated the association between distributive justice and job satisfaction. Further, organizational trust fully mediated the association between both distributive and procedural organizational justice and staff commitment toward the organization. [ABSTRACT FROM AUTHOR]