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1. Efficient quantification of HIV-1 in heparin plasma spiked with cultured HIV-1 by the Roche Cobas TaqMan and Abbott RealTime HIV-1 tests.

Author

Jagodzinski LL, Weston HR, Liu Y, O'Connell RJ, and Peel SA

Subjects
Anticoagulants metabolism, Automation methods, Heparin metabolism, Humans, HIV-1 isolation & purification, Plasma virology, Real-Time Polymerase Chain Reaction methods, Specimen Handling methods, Viral Load methods
Abstract

The current automated real-time HIV-1 viral load assays, the Roche Cobas AmpliPrep/Cobas TaqMan test and the Abbott RealTime test, are FDA cleared for use with EDTA plasma. We show that both real-time reverse transcription-PCR (RT-PCR) tests reliably quantify HIV-1 RNA in heparin plasma specimens spiked with HIV-1 isolate MN.

Published
2012
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