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3. Antiviral activity of natural phenolic compounds in complex at an allosteric site of SARS-CoV-2 papain-like protease

Author

Srinivasan, Vasundara, Brognaro, Hévila, Prabhu, Prince R., de Souza, Edmarcia Elisa, Günther, Sebastian, Reinke, Patrick Y. A., Lane, Thomas J., Ginn, Helen, Han, Huijong, Ewert, Wiebke, Sprenger, Janina, Koua, Faisal H. M., Falke, Sven, Werner, Nadine, Andaleeb, Hina, Ullah, Najeeb, Franca, Bruno Alves, Wang, Mengying, Barra, Angélica Luana C., Perbandt, Markus, Schwinzer, Martin, Schmidt, Christina, Brings, Lea, Lorenzen, Kristina, Schubert, Robin, Machado, Rafael Rahal Guaragna, Candido, Erika Donizette, Oliveira, Danielle Bruna Leal, Durigon, Edison Luiz, Niebling, Stephan, Garcia, Angelica Struve, Yefanov, Oleksandr, Lieske, Julia, Gelisio, Luca, Domaracky, Martin, Middendorf, Philipp, Groessler, Michael, Trost, Fabian, Galchenkova, Marina, Mashhour, Aida Rahmani, Saouane, Sofiane, Hakanpää, Johanna, Wolf, Markus, Alai, Maria Garcia, Turk, Dusan, Pearson, Arwen R., Chapman, Henry N., Hinrichs, Winfried, Wrenger, Carsten, Meents, Alke, and Betzel, Christian

Published
2022
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5. Megahertz serial crystallography

Author

Wiedorn, Max O, Oberthür, Dominik, Bean, Richard, Schubert, Robin, Werner, Nadine, Abbey, Brian, Aepfelbacher, Martin, Adriano, Luigi, Allahgholi, Aschkan, Al-Qudami, Nasser, Andreasson, Jakob, Aplin, Steve, Awel, Salah, Ayyer, Kartik, Bajt, Saša, Barák, Imrich, Bari, Sadia, Bielecki, Johan, Botha, Sabine, Boukhelef, Djelloul, Brehm, Wolfgang, Brockhauser, Sandor, Cheviakov, Igor, Coleman, Matthew A, Cruz-Mazo, Francisco, Danilevski, Cyril, Darmanin, Connie, Doak, R Bruce, Domaracky, Martin, Dörner, Katerina, Du, Yang, Fangohr, Hans, Fleckenstein, Holger, Frank, Matthias, Fromme, Petra, Gañán-Calvo, Alfonso M, Gevorkov, Yaroslav, Giewekemeyer, Klaus, Ginn, Helen Mary, Graafsma, Heinz, Graceffa, Rita, Greiffenberg, Dominic, Gumprecht, Lars, Göttlicher, Peter, Hajdu, Janos, Hauf, Steffen, Heymann, Michael, Holmes, Susannah, Horke, Daniel A, Hunter, Mark S, Imlau, Siegfried, Kaukher, Alexander, Kim, Yoonhee, Klyuev, Alexander, Knoška, Juraj, Kobe, Bostjan, Kuhn, Manuela, Kupitz, Christopher, Küpper, Jochen, Lahey-Rudolph, Janine Mia, Laurus, Torsten, Le Cong, Karoline, Letrun, Romain, Xavier, P Lourdu, Maia, Luis, Maia, Filipe RNC, Mariani, Valerio, Messerschmidt, Marc, Metz, Markus, Mezza, Davide, Michelat, Thomas, Mills, Grant, Monteiro, Diana CF, Morgan, Andrew, Mühlig, Kerstin, Munke, Anna, Münnich, Astrid, Nette, Julia, Nugent, Keith A, Nuguid, Theresa, Orville, Allen M, Pandey, Suraj, Pena, Gisel, Villanueva-Perez, Pablo, Poehlsen, Jennifer, Previtali, Gianpietro, Redecke, Lars, Riekehr, Winnie Maria, Rohde, Holger, Round, Adam, Safenreiter, Tatiana, Sarrou, Iosifina, Sato, Tokushi, Schmidt, Marius, Schmitt, Bernd, Schönherr, Robert, Schulz, Joachim, Sellberg, Jonas A, Seibert, M Marvin, and Seuring, Carolin

Subjects
Biochemistry and Cell Biology, Chemical Sciences, Biological Sciences
Abstract

The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.

Published
2018

7. Time-resolved crystallography of boric acid binding to the active site serine of the β-lactamase CTX-M-14 and subsequent 1,2-diol esterification

Author

Betzel, Christian, primary, Prester, Andreas, additional, Perbandt, Markus, additional, Galchenkova, Marina, additional, Oberthuer, Dominik, additional, Werner, Nadine, additional, Henkel, Alessandra, additional, Maracke, Julia, additional, Yefanov, Oleksandr, additional, Hakanpää, Johanna, additional, Pompidor, Guillaume, additional, Meyer, Jan, additional, Chapman, Henry, additional, Aepfelbacher, Martin, additional, Hinrichs, Winfried, additional, and Rohde, Holger, additional

Published
2023
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8. Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive

Author

Zielinski, Kara A, primary, Prester, Andreas, additional, Andaleeb, Hina, additional, Bui, Soi, additional, Yefanov, Oleksandr, additional, Catapano, Lucrezia, additional, Henkel, Alessandra, additional, Wiedorn, Max O., additional, Lorbeer, Olga, additional, Crosas, Eva, additional, Meyer, Jan, additional, Mariani, Valerio, additional, Domaracky, Martin, additional, White, Thomas A., additional, Fleckenstein, Holger, additional, Sarrou, Iosifina, additional, Werner, Nadine, additional, Betzel, Christian, additional, Rohde, Holger, additional, Aepfelbacher, Martin, additional, Chapman, Henry N., additional, Perbandt, Markus, additional, Steiner, Roberto A., additional, and Oberthuer, Dominik, additional

Published
2022
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9. Zeit und Person

Author

Werner, Nadine, Lindner, Burkhardt, editor, Küpper, Thomas, editor, and Skrandies, Timo, editor

Published
2011
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13. SARS-CoV-2 papain-like protease PLpro in complex with natural compounds reveal allosteric sites for antiviral drug design

Author

Srinivasan, Vasundara, primary, Brognaro, Hévila, additional, Prabhu, Prince R., additional, de Souza, Edmarcia Elisa, additional, Günther, Sebastian, additional, Reinke, Patrick Y. A., additional, Lane, Thomas J., additional, Ginn, Helen, additional, Han, Huijong, additional, Ewert, Wiebke, additional, Sprenger, Janina, additional, Koua, Faisal H. M., additional, Falke, Sven, additional, Werner, Nadine, additional, Andaleeb, Hina, additional, Ullah, Najeeb, additional, Franca, Bruno Alves, additional, Wang, Mengying, additional, Barra, Angélica Luana C, additional, Perbandt, Markus, additional, Schwinzer, Martin, additional, Schmidt, Christina, additional, Brings, Lea, additional, Lorenzen, Kristina, additional, Schubert, Robin, additional, Guaragna Machado, Rafael Rahal, additional, Candido, Erika Donizette, additional, Leal Oliveira, Danielle Bruna, additional, Durigon, Edison Luiz, additional, Yefanov, Oleksandr, additional, Lieske, Julia, additional, Gelisio, Luca, additional, Domaracky, Martin, additional, Middendorf, Philipp, additional, Groessler, Michael, additional, Trost, Fabian, additional, Galchenkova, Marina, additional, Saouane, Sofiane, additional, Hakanpää, Johanna, additional, Wolf, Markus, additional, Turk, Dusan, additional, Pearson, Arwen R., additional, Chapman, Henry N., additional, Hinrichs, Winfried, additional, Wrenger, Carsten, additional, Meents, Alke, additional, and Betzel, Christian, additional

Published
2021
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16. X-ray screening identifies active site and allosteric inhibitors of SARS-CoV-2 main protease

Author

Günther, Sebastian, Reinke, Patrick Y. A., Fernández-García, Yaiza, Lieske, Julia, Lane, Thomas J., Ginn, Helen M., Koua, Faisal H. M., Ehrt, Christiane, Ewert, Wiebke, Oberthuer, Dominik, Yefanov, Oleksandr, Meier, Susanne, Lorenzen, Kristina, Krichel, Boris, Kopicki, Janine-Denise, Gelisio, Luca, Brehm, Wolfgang, Dunkel, Ilona, Seychell, Brandon, Gieseler, Henry, Norton-Baker, Brenna, Escudero-Pérez, Beatriz, Domaracky, Martin, Saouane, Sofiane, Tolstikova, Alexandra, White, Thomas A., Hänle, Anna, Groessler, Michael, Fleckenstein, Holger, Trost, Fabian, Galchenkova, Marina, Gevorkov, Yaroslav, Li, Chufeng, Awel, Salah, Peck, Ariana, Barthelmess, Miriam, Schlünzen, Frank, Lourdu Xavier, P., Werner, Nadine, Andaleeb, Hina, Ullah, Najeeb, Falke, Sven, Srinivasan, Vasundara, França, Bruno Alves, Schwinzer, Martin, Brognaro, Hévila, Rogers, Cromarte, Melo, Diogo, Zaitseva-Doyle, Joanna J., Knoska, Juraj, Peña-Murillo, Gisel E., Mashhour, Aida Rahmani, Hennicke, Vincent, Fischer, Pontus, Hakanpää, Johanna, Meyer, Jan, Gribbon, Philip, Ellinger, Bernhard, Kuzikov, Maria, Wolf, Markus, Beccari, Andrea R., Bourenkov, Gleb, von Stetten, David, Pompidor, Guillaume, Bento, Isabel, Panneerselvam, Saravanan, Karpics, Ivars, Schneider, Thomas R., Garcia-Alai, Maria Marta, Niebling, Stephan, Günther, Christian, Schmidt, Christina, Schubert, Robin, Han, Huijong, Boger, Juliane, Monteiro, Diana C. F., Zhang, Linlin, Sun, Xinyuanyuan, Pletzer-Zelgert, Jonathan, Wollenhaupt, Jan, Feiler, Christian G., Weiss, Manfred S., Schulz, Eike-Christian, Mehrabi, Pedram, Karničar, Katarina, Usenik, Aleksandra, Loboda, Jure, Tidow, Henning, Chari, Ashwin, Hilgenfeld, Rolf, Uetrecht, Charlotte, Cox, Russell, Zaliani, Andrea, Beck, Tobias, Rarey, Matthias, Günther, Stephan, Turk, Dusan, Hinrichs, Winfried, Chapman, Henry N., Pearson, Arwen R., Betzel, Christian, Meents, Alke, Günther, Sebastian, Reinke, Patrick Y. A., Fernández-García, Yaiza, Lieske, Julia, Lane, Thomas J., Ginn, Helen M., Koua, Faisal H. M., Ehrt, Christiane, Ewert, Wiebke, Oberthuer, Dominik, Yefanov, Oleksandr, Meier, Susanne, Lorenzen, Kristina, Krichel, Boris, Kopicki, Janine-Denise, Gelisio, Luca, Brehm, Wolfgang, Dunkel, Ilona, Seychell, Brandon, Gieseler, Henry, Norton-Baker, Brenna, Escudero-Pérez, Beatriz, Domaracky, Martin, Saouane, Sofiane, Tolstikova, Alexandra, White, Thomas A., Hänle, Anna, Groessler, Michael, Fleckenstein, Holger, Trost, Fabian, Galchenkova, Marina, Gevorkov, Yaroslav, Li, Chufeng, Awel, Salah, Peck, Ariana, Barthelmess, Miriam, Schlünzen, Frank, Lourdu Xavier, P., Werner, Nadine, Andaleeb, Hina, Ullah, Najeeb, Falke, Sven, Srinivasan, Vasundara, França, Bruno Alves, Schwinzer, Martin, Brognaro, Hévila, Rogers, Cromarte, Melo, Diogo, Zaitseva-Doyle, Joanna J., Knoska, Juraj, Peña-Murillo, Gisel E., Mashhour, Aida Rahmani, Hennicke, Vincent, Fischer, Pontus, Hakanpää, Johanna, Meyer, Jan, Gribbon, Philip, Ellinger, Bernhard, Kuzikov, Maria, Wolf, Markus, Beccari, Andrea R., Bourenkov, Gleb, von Stetten, David, Pompidor, Guillaume, Bento, Isabel, Panneerselvam, Saravanan, Karpics, Ivars, Schneider, Thomas R., Garcia-Alai, Maria Marta, Niebling, Stephan, Günther, Christian, Schmidt, Christina, Schubert, Robin, Han, Huijong, Boger, Juliane, Monteiro, Diana C. F., Zhang, Linlin, Sun, Xinyuanyuan, Pletzer-Zelgert, Jonathan, Wollenhaupt, Jan, Feiler, Christian G., Weiss, Manfred S., Schulz, Eike-Christian, Mehrabi, Pedram, Karničar, Katarina, Usenik, Aleksandra, Loboda, Jure, Tidow, Henning, Chari, Ashwin, Hilgenfeld, Rolf, Uetrecht, Charlotte, Cox, Russell, Zaliani, Andrea, Beck, Tobias, Rarey, Matthias, Günther, Stephan, Turk, Dusan, Hinrichs, Winfried, Chapman, Henry N., Pearson, Arwen R., Betzel, Christian, and Meents, Alke

Abstract

The coronavirus disease (COVID-19) caused by SARS-CoV-2 is creating tremendous human suffering. To date, no effective drug is available to directly treat the disease. In a search for a drug against COVID-19, we have performed a high-throughput X-ray crystallographic screen of two repurposing drug libraries against the SARS-CoV-2 main protease (Mpro), which is essential for viral replication. In contrast to commonly applied X-ray fragment screening experiments with molecules of low complexity, our screen tested alreadyapproved drugs and drugs in clinical trials. From the three-dimensional protein structures, we identified 37 compounds that bind to Mpro. In subsequent cell-based viral reduction assays, one peptidomimetic and six nonpeptidic compounds showed antiviral activity at nontoxic concentrations. We identified two allosteric binding sites representing attractive targets for drug development against SARS-CoV-2.

Published
2021

17. X-ray screening identifies active site and allosteric inhibitors of SARS-CoV-2 main protease

Author

Günther, Sebastian, primary, Reinke, Patrick Y. A., additional, Fernández-García, Yaiza, additional, Lieske, Julia, additional, Lane, Thomas J., additional, Ginn, Helen M., additional, Koua, Faisal H. M., additional, Ehrt, Christiane, additional, Ewert, Wiebke, additional, Oberthuer, Dominik, additional, Yefanov, Oleksandr, additional, Meier, Susanne, additional, Lorenzen, Kristina, additional, Krichel, Boris, additional, Kopicki, Janine-Denise, additional, Gelisio, Luca, additional, Brehm, Wolfgang, additional, Dunkel, Ilona, additional, Seychell, Brandon, additional, Gieseler, Henry, additional, Norton-Baker, Brenna, additional, Escudero-Pérez, Beatriz, additional, Domaracky, Martin, additional, Saouane, Sofiane, additional, Tolstikova, Alexandra, additional, White, Thomas A., additional, Hänle, Anna, additional, Groessler, Michael, additional, Fleckenstein, Holger, additional, Trost, Fabian, additional, Galchenkova, Marina, additional, Gevorkov, Yaroslav, additional, Li, Chufeng, additional, Awel, Salah, additional, Peck, Ariana, additional, Barthelmess, Miriam, additional, Schlünzen, Frank, additional, Lourdu Xavier, P., additional, Werner, Nadine, additional, Andaleeb, Hina, additional, Ullah, Najeeb, additional, Falke, Sven, additional, Srinivasan, Vasundara, additional, França, Bruno Alves, additional, Schwinzer, Martin, additional, Brognaro, Hévila, additional, Rogers, Cromarte, additional, Melo, Diogo, additional, Zaitseva-Doyle, Joanna J., additional, Knoska, Juraj, additional, Peña-Murillo, Gisel E., additional, Mashhour, Aida Rahmani, additional, Hennicke, Vincent, additional, Fischer, Pontus, additional, Hakanpää, Johanna, additional, Meyer, Jan, additional, Gribbon, Philip, additional, Ellinger, Bernhard, additional, Kuzikov, Maria, additional, Wolf, Markus, additional, Beccari, Andrea R., additional, Bourenkov, Gleb, additional, von Stetten, David, additional, Pompidor, Guillaume, additional, Bento, Isabel, additional, Panneerselvam, Saravanan, additional, Karpics, Ivars, additional, Schneider, Thomas R., additional, Garcia-Alai, Maria Marta, additional, Niebling, Stephan, additional, Günther, Christian, additional, Schmidt, Christina, additional, Schubert, Robin, additional, Han, Huijong, additional, Boger, Juliane, additional, Monteiro, Diana C. F., additional, Zhang, Linlin, additional, Sun, Xinyuanyuan, additional, Pletzer-Zelgert, Jonathan, additional, Wollenhaupt, Jan, additional, Feiler, Christian G., additional, Weiss, Manfred S., additional, Schulz, Eike-Christian, additional, Mehrabi, Pedram, additional, Karničar, Katarina, additional, Usenik, Aleksandra, additional, Loboda, Jure, additional, Tidow, Henning, additional, Chari, Ashwin, additional, Hilgenfeld, Rolf, additional, Uetrecht, Charlotte, additional, Cox, Russell, additional, Zaliani, Andrea, additional, Beck, Tobias, additional, Rarey, Matthias, additional, Günther, Stephan, additional, Turk, Dusan, additional, Hinrichs, Winfried, additional, Chapman, Henry N., additional, Pearson, Arwen R., additional, Betzel, Christian, additional, and Meents, Alke, additional

Published
2021
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18. The Komagataeibacter europaeus GqqA is the prototype of a novel bifunctional N-Acyl-homoserine lactone acylase with prephenate dehydratase activity

Author

Werner, Nadine, primary, Petersen, Katrin, additional, Vollstedt, Christel, additional, Garcia, Pablo Perez, additional, Chow, Jennifer, additional, Ferrer, Manuel, additional, Fernandes-Lopez, Laura, additional, Falke, Sven, additional, Perbandt, Markus, additional, Hinrichs, Winfried, additional, Betzel, Christian, additional, and Streit, Wolfgang R., additional

Published
2021
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20. Enhancing protective microglial activities with a dual function TREM 2 antibody to the stalk region

Author

Kai Schlepckow, Kathryn M Monroe, Gernot Kleinberger, Ludovico Cantuti‐Castelvetri, Samira Parhizkar, Dan Xia, Michael Willem, Georg Werner, Nadine Pettkus, Bettina Brunner, Alice Sülzen, Brigitte Nuscher, Heike Hampel, Xianyuan Xiang, Regina Feederle, Sabina Tahirovic, Joshua I Park, Rachel Prorok, Cathal Mahon, Chun‐Chi Liang, Ju Shi, Do Jin Kim, Hanna Sabelström, Fen Huang, Gilbert Di Pao

Published
2020
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21. Inhibition of SARS-CoV-2 main protease by allosteric drug-binding

Author

Günther, Sebastian, primary, Reinke, Patrick Y. A., additional, Fernández-García, Yaiza, additional, Lieske, Julia, additional, Lane, Thomas J., additional, Ginn, Helen M., additional, Koua, Faisal H. M., additional, Ehrt, Christiane, additional, Ewert, Wiebke, additional, Oberthuer, Dominik, additional, Yefanov, Oleksandr, additional, Meier, Susanne, additional, Lorenzen, Kristina, additional, Krichel, Boris, additional, Kopicki, Janine-Denise, additional, Gelisio, Luca, additional, Brehm, Wolfgang, additional, Dunkel, Ilona, additional, Seychell, Brandon, additional, Gieseler, Henry, additional, Norton-Baker, Brenna, additional, Escudero-Pérez, Beatriz, additional, Domaracky, Martin, additional, Saouane, Sofiane, additional, Tolstikova, Alexandra, additional, White, Thomas A., additional, Hänle, Anna, additional, Groessler, Michael, additional, Fleckenstein, Holger, additional, Trost, Fabian, additional, Galchenkova, Marina, additional, Gevorkov, Yaroslav, additional, Li, Chufeng, additional, Awel, Salah, additional, Peck, Ariana, additional, Barthelmess, Miriam, additional, Schlünzen, Frank, additional, Xavier, P. Lourdu, additional, Werner, Nadine, additional, Andaleeb, Hina, additional, Ullah, Najeeb, additional, Falke, Sven, additional, Srinivasan, Vasundara, additional, Franca, Bruno Alves, additional, Schwinzer, Martin, additional, Brognaro, Hévila, additional, Rogers, Cromarte, additional, Melo, Diogo, additional, Zaitsev-Doyle, Jo J., additional, Knoska, Juraj, additional, Peña Murillo, Gisel E., additional, Mashhour, Aida Rahmani, additional, Guicking, Filip, additional, Hennicke, Vincent, additional, Fischer, Pontus, additional, Hakanpää, Johanna, additional, Meyer, Jan, additional, Gribbon, Phil, additional, Ellinger, Bernhard, additional, Kuzikov, Maria, additional, Wolf, Markus, additional, Beccari, Andrea R., additional, Bourenkov, Gleb, additional, Stetten, David von, additional, Pompidor, Guillaume, additional, Bento, Isabel, additional, Panneerselvam, Saravanan, additional, Karpics, Ivars, additional, Schneider, Thomas R., additional, Garcia Alai, Maria Marta, additional, Niebling, Stephan, additional, Günther, Christian, additional, Schmidt, Christina, additional, Schubert, Robin, additional, Han, Huijong, additional, Boger, Juliane, additional, Monteiro, Diana C. F., additional, Zhang, Linlin, additional, Sun, Xinyuanyuan, additional, Pletzer-Zelgert, Jonathan, additional, Wollenhaupt, Jan, additional, Feiler, Christian G., additional, Weiss, Manfred S., additional, Schulz, Eike-Christian, additional, Mehrabi, Pedram, additional, Karničar, Katarina, additional, Usenik, Aleksandra, additional, Loboda, Jure, additional, Tidow, Henning, additional, Chari, Ashwin, additional, Hilgenfeld, Rolf, additional, Uetrecht, Charlotte, additional, Cox, Russell, additional, Zaliani, Andrea, additional, Beck, Tobias, additional, Rarey, Matthias, additional, Günther, Stephan, additional, Turk, Dusan, additional, Hinrichs, Winfried, additional, Chapman, Henry N., additional, Pearson, Arwen R., additional, Betzel, Christian, additional, and Meents, Alke, additional

Published
2020
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23. The induction mechanism of the flavonoid‐responsive regulator FrrA.

Author

Werner, Nadine, Werten, Sebastiaan, Hoppen, Jens, Palm, Gottfried J., Göttfert, Michael, and Hinrichs, Winfried

Subjects
*SMALL-angle X-ray scattering, *FLAVONOIDS, *LIGAND binding (Biochemistry), *SINGLE molecules, *GENETIC programming, *CRYSTAL structure, *BRADYRHIZOBIUM
Abstract

Bradyrhizobium diazoefficiens, a bacterial symbiont of soybean and other leguminous plants, enters a nodulation‐promoting genetic programme in the presence of host‐produced flavonoids and related signalling compounds. Here, we describe the crystal structure of an isoflavonoid‐responsive regulator (FrrA) from Bradyrhizobium, as well as cocrystal structures with inducing and noninducing ligands (genistein and naringenin, respectively). The structures reveal a TetR‐like fold whose DNA‐binding domain is capable of adopting a range of orientations. A single molecule of either genistein or naringenin is asymmetrically bound in a central cavity of the FrrA homodimer, mainly via C–H contacts to the π‐system of the ligands. Strikingly, however, the interaction does not provoke any conformational changes in the repressor. Both the flexible positioning of the DNA‐binding domain and the absence of structural change upon ligand binding are corroborated by small‐angle X‐ray scattering (SAXS) experiments in solution. Together with a model of the promoter‐bound state of FrrA our results suggest that inducers act as a wedge, preventing the DNA‐binding domains from moving close enough together to interact with successive positions of the major groove of the palindromic operator. [ABSTRACT FROM AUTHOR]

Published
2022
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24. Catalytic cleavage of HEAT and subsequent covalent binding of the tetralone moiety by the SARS-CoV-2 main protease

Author

Günther, Sebastian, primary, Reinke, Patrick Y. A., additional, Oberthuer, Dominik, additional, Yefanov, Oleksandr, additional, Ginn, Helen, additional, Meier, Susanne, additional, Lane, Thomas J., additional, Lorenzen, Kristina, additional, Gelisio, Luca, additional, Brehm, Wolfgang, additional, Dunkel, Illona, additional, Domaracky, Martin, additional, Saouane, Sofiane, additional, Lieske, Julia, additional, Ehrt, Christiane, additional, Koua, Faisal, additional, Tolstikova, Alexandra, additional, White, Thomas A., additional, Groessler, Michael, additional, Fleckenstein, Holger, additional, Trost, Fabian, additional, Galchenkova, Marina, additional, Gevorkov, Yaroslav, additional, Li, Chufeng, additional, Awel, Salah, additional, Peck, Ariana, additional, Xavier, P. Lourdu, additional, Barthelmess, Miriam, additional, Schlünzen, Frank, additional, Werner, Nadine, additional, Andaleeb, Hina, additional, Ullah, Najeeb, additional, Falke, Sven, additional, Franca, Bruno Alves, additional, Schwinzer, Martin, additional, Brognaro, Hévila, additional, Seychell, Brandon, additional, Gieseler, Henry, additional, Melo, Diogo, additional, Zaitsev-Doyle, Jo J., additional, Norton-Baker, Brenna, additional, Knoska, Juraj, additional, Esperanza, Gisel, additional, Mashhour, Aida Rahmani, additional, Guicking, Filip, additional, Hennicke, Vincent, additional, Fischer, Pontus, additional, Rogers, Cromarte, additional, Monteiro, Diana C. F., additional, Hakanpää, Johanna, additional, Meyer, Jan, additional, Noei, Heshmat, additional, Gribbon, Phil, additional, Ellinger, Bernhard, additional, Kuzikov, Maria, additional, Wolf, Markus, additional, Zhang, Linlin, additional, Sun, Xinyuanyuan, additional, Pletzer-Zelgert, Jonathan, additional, Wollenhaupt, Jan, additional, Feiler, Christian, additional, Weiss, Manfred, additional, Schulz, Eike-Christian, additional, Mehrabi, Pedram, additional, Schmidt, Christina, additional, Schubert, Robin, additional, Han, Huijong, additional, Krichel, Boris, additional, Fernández-García, Yaiza, additional, Escudero-Pérez, Beatriz, additional, Günther, Stephan, additional, Turk, Dusan, additional, Uetrecht, Charlotte, additional, Beck, Tobias, additional, Tidow, Henning, additional, Chari, Ashwin, additional, Zaliani, Andrea, additional, Rarey, Matthias, additional, Cox, Russell, additional, Hilgenfeld, Rolf, additional, Chapman, Henry N., additional, Pearson, Arwen R., additional, Betzel, Christian, additional, and Meents, Alke, additional

Published
2020
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27. Low temporal dynamics of mycosporine‐like amino acids in benthic cyanobacteria from an alpine lake.

Author

Werner, Nadine, Orfanoudaki, Maria, Hartmann, Anja, Ganzera, Markus, and Sommaruga, Ruben

Subjects
*CYANOBACTERIAL toxins, *SOLAR ultraviolet radiation, *LIQUID chromatography-mass spectrometry, *AMINO acids, *HIGH performance liquid chromatography, *NUCLEAR magnetic resonance
Abstract

Cyanobacteria are one of the oldest organisms on Earth and they originated at a time when damaging ultraviolet (UV) C radiation still reached the surface. Their long evolution led to several adaptations to avoid deleterious effects caused by exposure to solar UV radiation. Synthesis of sunscreen substances, such as mycosporine‐like amino acids (MAAs), allows them to photosynthesise with reduced risk of cell damage. The interplay of solar UV radiation and MAAs is well documented for cyanobacteria in the plankton realm, but little is known for those in the benthic realm, particularly of clear alpine lakes.Here, we assessed the temporal dynamics of MAAs in the benthic algal community of one clear alpine lake dominated by cyanobacteria during the ice‐free season and along a depth gradient using state‐of‐the‐art analytical methods (high‐performance liquid chromatography, nuclear magnetic resonance, liquid chromatography–mass spectrometry). We differentiated between the epilithic cyanobacterial community and the overlying loosely attached filamentous cyanobacteria, as we expected they will have an important shielding/shading effect on the former. We hypothesised that in contrast to the case of phytoplankton, benthic cyanobacteria will show less pronounced temporal changes in MAAs concentration in response to changes in solar UV exposure.Three UV‐absorbing substances were present in both types of communities, whereby all were unknown. The chemical structure of the dominant unknown substance (maximum absorption at 334 nm) resulted in the identification of a novel MAA that we named aplysiapalythine‐D for its similarity to the previously described aplysiapalythine‐C.Chlorophyll‐a‐specific MAA concentrations for epilithic and filamentous cyanobacteria showed a significant decrease with depth, although only traces were found in the former community. The temporal dynamics in MAA concentrations of filamentous cyanobacteria showed no significant variations during the ice‐free season.Our result on the low temporal MAA dynamics agrees with the reduced growth rates of benthic cyanobacteria reported for cold ecosystems. The permanent presence of this community, which is adapted to the high UV levels characteristic of clear alpine lakes, probably represents the most important primary producers of these ecosystems. [ABSTRACT FROM AUTHOR]

Published
2021
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28. Megahertz serial crystallography

Author

Wiedorn, Max Oliver, Oberthür, Dominik, Bean, Richard, Schubert, Robin, Werner, Nadine, Abbey, Brian, Aepfelbacher, Martin, Adriano, Luigi, Allahgholi, Aschkan, Al-Qudami, Nasser, Andreasson, Jakob, Shelby, Megan L., Shoemann, Robert L., Sikorski, Marcin, Silenzi, Alessandro, Stan, Claudiu A., Shi, Xintian, Stern, Stephan, Sztuk-Dambietz, Jolanta, Szuba, Janusz, Tolstikova, Aleksandra, Aplin, Steven, Trebbin, Martin, Trunk, Ulrich, Vagovic, Patrik, Ve, Thomas, Weinhausen, Britta, White, Thomas A., Wrona, Krzysztof, Xu, Chen, Yefanov, Oleksandr, Zatsepin, Nadia, Awel, Salah, Zhang, Jiaguo, Perbandt, Markus, Mancuso, Adrian P., Betzel, Christian, Chapman, Henry N., Banty, Anton, Ayyer, Kartik, Bajt, Saša, Barák, Imrich, Bari, Sadia, Bielecki, Johan, Botha, Sabine, Boukhelef, Djelloul, Brehm, Wolfgang, Brockhauser, Sandor, Cheviakov, Igor, Coleman, Matthew A., Cruz-Mazo, Francisco, Danilevski, Cyril, Darmanin, Connie, Doak, R. Bruce, Domaracky, Martin, Dörner, Katerina Henrike, Du, Yang, Fangohr, Hans, Fleckenstein, Holger, Frank, Matthias, Fromme, Petra, Gañán-Calvo, Alfonso M., Gevorkov, Yaroslav, Giewekemeyer, Klaus, Ginn, Helen Mary, Graafsma, Heinz, Graceffa, Rita, Greiffenberg, Dominic, Gumprecht, Lars, Göttlicher, Peter, Hajdu, János, Hauf, Steffen, Heymann, Michael, Holmes, Susannah, Horke, Daniel A., Hunter, Mark S., Imlau, Siegfried, Kaukher, Alexander, Kim, Yoonhee, Klyuev, Alexander, Knoška, Juraj, Kobe, Bostjan, Kuhn, Manuela, Kupitz, Christopher, Küpper, Jochen, Lahey-Rudolph, Janine Mia, Laurus, Torsten, Le Cong, Karoline, Letrun, Romain, Xavier, Paulrajpillai Lourdu, Maia, Luis, Maia, Filipe R. N. C., Mariani, Valerio, Messerschmidt, Marc, Metz, Markus, Mezza, Davide, Michelat, Thomas, Mills, Grant, Monteiro, Diana C.F., Morgan, Andrew J., Mühlig, Kerstin, Munke, Anna, Münnich, Astrid, Nette, Julia, Nugent, Keith A., Nuguid, Theresa, Orville, Allen M., Pandey, Suraj, Pena, Gisel, Vilanueva-Pérez, Pablo, Pöhlsen, Jennifer, Previtali, Gianpietro, Redecke, Lars, Riekehr, Winnie Maria, Rohde, Holger, Round, Adam R., Safenreiter, Tatiana, Sarrou, Iosifina, Sato, Tokushi, Schmidt, Marius, Schmitt, Bernd, Schönherr, Robert, Schulz, Joachim, Sellberg, Jonas Alexander, Seibert, M. Marvin, Seuring, Carolin, Wiedorn, Max Oliver, Oberthür, Dominik, Bean, Richard, Schubert, Robin, Werner, Nadine, Abbey, Brian, Aepfelbacher, Martin, Adriano, Luigi, Allahgholi, Aschkan, Al-Qudami, Nasser, Andreasson, Jakob, Shelby, Megan L., Shoemann, Robert L., Sikorski, Marcin, Silenzi, Alessandro, Stan, Claudiu A., Shi, Xintian, Stern, Stephan, Sztuk-Dambietz, Jolanta, Szuba, Janusz, Tolstikova, Aleksandra, Aplin, Steven, Trebbin, Martin, Trunk, Ulrich, Vagovic, Patrik, Ve, Thomas, Weinhausen, Britta, White, Thomas A., Wrona, Krzysztof, Xu, Chen, Yefanov, Oleksandr, Zatsepin, Nadia, Awel, Salah, Zhang, Jiaguo, Perbandt, Markus, Mancuso, Adrian P., Betzel, Christian, Chapman, Henry N., Banty, Anton, Ayyer, Kartik, Bajt, Saša, Barák, Imrich, Bari, Sadia, Bielecki, Johan, Botha, Sabine, Boukhelef, Djelloul, Brehm, Wolfgang, Brockhauser, Sandor, Cheviakov, Igor, Coleman, Matthew A., Cruz-Mazo, Francisco, Danilevski, Cyril, Darmanin, Connie, Doak, R. Bruce, Domaracky, Martin, Dörner, Katerina Henrike, Du, Yang, Fangohr, Hans, Fleckenstein, Holger, Frank, Matthias, Fromme, Petra, Gañán-Calvo, Alfonso M., Gevorkov, Yaroslav, Giewekemeyer, Klaus, Ginn, Helen Mary, Graafsma, Heinz, Graceffa, Rita, Greiffenberg, Dominic, Gumprecht, Lars, Göttlicher, Peter, Hajdu, János, Hauf, Steffen, Heymann, Michael, Holmes, Susannah, Horke, Daniel A., Hunter, Mark S., Imlau, Siegfried, Kaukher, Alexander, Kim, Yoonhee, Klyuev, Alexander, Knoška, Juraj, Kobe, Bostjan, Kuhn, Manuela, Kupitz, Christopher, Küpper, Jochen, Lahey-Rudolph, Janine Mia, Laurus, Torsten, Le Cong, Karoline, Letrun, Romain, Xavier, Paulrajpillai Lourdu, Maia, Luis, Maia, Filipe R. N. C., Mariani, Valerio, Messerschmidt, Marc, Metz, Markus, Mezza, Davide, Michelat, Thomas, Mills, Grant, Monteiro, Diana C.F., Morgan, Andrew J., Mühlig, Kerstin, Munke, Anna, Münnich, Astrid, Nette, Julia, Nugent, Keith A., Nuguid, Theresa, Orville, Allen M., Pandey, Suraj, Pena, Gisel, Vilanueva-Pérez, Pablo, Pöhlsen, Jennifer, Previtali, Gianpietro, Redecke, Lars, Riekehr, Winnie Maria, Rohde, Holger, Round, Adam R., Safenreiter, Tatiana, Sarrou, Iosifina, Sato, Tokushi, Schmidt, Marius, Schmitt, Bernd, Schönherr, Robert, Schulz, Joachim, Sellberg, Jonas Alexander, Seibert, M. Marvin, and Seuring, Carolin

Abstract

The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.

Published
2018

29. Megahertz serial crystallography

Author

Wiedorn, Max O., Oberthuer, Dominik, Bean, Richard, Schubert, Robin, Werner, Nadine, Abbey, Brian, Aepfelbacher, Martin, Adriano, Luigi, Allahgholi, Aschkan, Al-Qudami, Nasser, Andreasson, Jakob, Aplin, Steve, Awel, Salah, Ayyer, Kartik, Bajt, Sasa, Barak, Imrich, Bari, Sadia, Bielecki, Johan, Botha, Sabine, Boukhelef, Djelloul, Brehm, Wolfgang, Brockhauser, Sandor, Cheviakov, Igor, Coleman, Matthew A., Cruz-Mazo, Francisco, Danilevski, Cyril, Darmanin, Connie, Doak, R. Bruce, Domaracky, Martin, Doerner, Katerina, Du, Yang, Fangohr, Hans, Fleckenstein, Holger, Frank, Matthias, Fromme, Petra, Ganan-Calvo, Alfonso M., Gevorkov, Yaroslav, Giewekemeyer, Klaus, Ginn, Helen Mary, Graafsma, Heinz, Graceffa, Rita, Greiffenberg, Dominic, Gumprecht, Lars, Goettlicher, Peter, Hajdu, Janos, Hauf, Steffen, Heymann, Michael, Holmes, Susannah, Horke, Daniel A., Hunter, Mark S., Imlau, Siegfried, Kaukher, Alexander, Kim, Yoonhee, Klyuev, Alexander, Knoska, Juraj, Kobe, Bostjan, Kuhn, Manuela, Kupitz, Christopher, Kueper, Jochen, Lahey-Rudolph, Janine Mia, Laurus, Torsten, Le Cong, Karoline, Letrun, Romain, Xavier, P. Lourdu, Maia, Luis, Maia, Filipe R.N.C., Mariani, Valerio, Messerschmidt, Marc, Metz, Markus, Mezza, Davide, Michelat, Thomas, Mills, Grant, Monteiro, Diana C. F., Morgan, Andrew, Mühlig, Kerstin, Munke, Anna, Muennich, Astrid, Nette, Julia, Nugent, Keith A., Nuguid, Theresa, Orville, Allen M., Pandey, Suraj, Pena, Gisel, Villanueva-Perez, Pablo, Poehlsen, Jennifer, Previtali, Gianpietro, Redecke, Lars, Riekehr, Winnie Maria, Rohde, Holger, Round, Adam, Safenreiter, Tatiana, Sarrou, Iosifina, Sato, Tokushi, Schmidt, Marius, Schmitt, Bernd, Schoenherr, Robert, Schulz, Joachim, Sellberg, Jonas A., Seibert, M. Marvin, Seuring, Carolin, Shelby, Megan L., Shoeman, Robert L., Sikorski, Marcin, Silenzi, Alessandro, Stan, Claudiu A., Shi, Xintian, Stern, Stephan, Sztuk-Dambietz, Jola, Szuba, Janusz, Tolstikova, Aleksandra, Trebbin, Martin, Trunk, Ulrich, Vagovic, Patrik, Ve, Thomas, Weinhausen, Britta, White, Thomas A., Wrona, Krzysztof, Xu, Chen, Yefanov, Oleksandr, Zatsepin, Nadia, Zhang, Jiaguo, Perbandt, Markus, Mancuso, Adrian P., Betzel, Christian, Chapman, Henry, Barty, Anton, Wiedorn, Max O., Oberthuer, Dominik, Bean, Richard, Schubert, Robin, Werner, Nadine, Abbey, Brian, Aepfelbacher, Martin, Adriano, Luigi, Allahgholi, Aschkan, Al-Qudami, Nasser, Andreasson, Jakob, Aplin, Steve, Awel, Salah, Ayyer, Kartik, Bajt, Sasa, Barak, Imrich, Bari, Sadia, Bielecki, Johan, Botha, Sabine, Boukhelef, Djelloul, Brehm, Wolfgang, Brockhauser, Sandor, Cheviakov, Igor, Coleman, Matthew A., Cruz-Mazo, Francisco, Danilevski, Cyril, Darmanin, Connie, Doak, R. Bruce, Domaracky, Martin, Doerner, Katerina, Du, Yang, Fangohr, Hans, Fleckenstein, Holger, Frank, Matthias, Fromme, Petra, Ganan-Calvo, Alfonso M., Gevorkov, Yaroslav, Giewekemeyer, Klaus, Ginn, Helen Mary, Graafsma, Heinz, Graceffa, Rita, Greiffenberg, Dominic, Gumprecht, Lars, Goettlicher, Peter, Hajdu, Janos, Hauf, Steffen, Heymann, Michael, Holmes, Susannah, Horke, Daniel A., Hunter, Mark S., Imlau, Siegfried, Kaukher, Alexander, Kim, Yoonhee, Klyuev, Alexander, Knoska, Juraj, Kobe, Bostjan, Kuhn, Manuela, Kupitz, Christopher, Kueper, Jochen, Lahey-Rudolph, Janine Mia, Laurus, Torsten, Le Cong, Karoline, Letrun, Romain, Xavier, P. Lourdu, Maia, Luis, Maia, Filipe R.N.C., Mariani, Valerio, Messerschmidt, Marc, Metz, Markus, Mezza, Davide, Michelat, Thomas, Mills, Grant, Monteiro, Diana C. F., Morgan, Andrew, Mühlig, Kerstin, Munke, Anna, Muennich, Astrid, Nette, Julia, Nugent, Keith A., Nuguid, Theresa, Orville, Allen M., Pandey, Suraj, Pena, Gisel, Villanueva-Perez, Pablo, Poehlsen, Jennifer, Previtali, Gianpietro, Redecke, Lars, Riekehr, Winnie Maria, Rohde, Holger, Round, Adam, Safenreiter, Tatiana, Sarrou, Iosifina, Sato, Tokushi, Schmidt, Marius, Schmitt, Bernd, Schoenherr, Robert, Schulz, Joachim, Sellberg, Jonas A., Seibert, M. Marvin, Seuring, Carolin, Shelby, Megan L., Shoeman, Robert L., Sikorski, Marcin, Silenzi, Alessandro, Stan, Claudiu A., Shi, Xintian, Stern, Stephan, Sztuk-Dambietz, Jola, Szuba, Janusz, Tolstikova, Aleksandra, Trebbin, Martin, Trunk, Ulrich, Vagovic, Patrik, Ve, Thomas, Weinhausen, Britta, White, Thomas A., Wrona, Krzysztof, Xu, Chen, Yefanov, Oleksandr, Zatsepin, Nadia, Zhang, Jiaguo, Perbandt, Markus, Mancuso, Adrian P., Betzel, Christian, Chapman, Henry, and Barty, Anton

Abstract

The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a beta-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.

Published
2018
Full Text
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30. Paper & Pattern.

Author

Werner, Nadine

Subjects
BOOKBINDERS, WORK in art
Published
2020

32. Benjamin Lektüren. Zur internationalen Rezeption. Ein Symposium des Walter Benjamin Archivs, AdK, Berlin.

Author

Werner, Nadine

Subjects
*TRANSLATING & interpreting, *TRANSLATING & interpreting of philosophical works, *PHILOSOPHY of culture, *CONFERENCES & conventions, *INTELLECTUAL life, *WESTERN civilization, EAST Asian civilization -- Western influences, MIDDLE Eastern civilization
Abstract

The article presents a report on a conference on the philosopher and cultural critic Walter Benjamin, held in Berlin, Germany, from September 18-20, 2013. The papers presented revolved around the translation and reception of Benjamin's works in various locales, including in East Asia, the Middle East, and Latin America.

Published
2013

33. Megahertz serial crystallography

Author

Wiedorn, Max Oliver, Oberth��r, Dominik, Bean, Richard, Schubert, Robin, Werner, Nadine, Abbey, Brian, Aepfelbacher, Martin, Adriano, Luigi, Allahgholi, Aschkan, Al-Qudami, Nasser, Andreasson, Jakob, Horke, Daniel A., Hunter, Mark S., Imlau, Siegfried, Kaukher, Alexander, Kim, Yoonhee, Klyuev, Alexander, Kno��ka, Juraj, Kobe, Bostjan, Kuhn, Manuela, Kupitz, Christopher, M��hlig, Kerstin, K��pper, Jochen, Lahey-Rudolph, Janine Mia, Laurus, Torsten, Le Cong, Karoline, Letrun, Romain, Xavier, Paulrajpillai Lourdu, Maia, Luis, Maia, Filipe R. N. C., Mariani, Valerio, Messerschmidt, Marc, Munke, Anna, Metz, Markus, Mezza, Davide, Michelat, Thomas, Mills, Grant, Monteiro, Diana C.F., Morgan, Andrew J., M��nnich, Astrid, Nette, Julia, Nugent, Keith A., Nuguid, Theresa, Orville, Allen M., Pandey, Suraj, Pena, Gisel, Villanueva-Perez, Pablo, Shelby, Megan L., P��hlsen, Jennifer, Previtali, Gianpietro, Redecke, Lars, Riekehr, Winnie Maria, Rohde, Holger, Round, Adam R., Safenreiter, Tatiana, Sarrou, Iosifina, Sato, Tokushi, Schmidt, Marius, Shoemann, Robert L., Schmitt, Bernd, Sch��nherr, Robert, Schulz, Joachim, Sellberg, Jonas Alexander, Seibert, M. Marvin, Seuring, Carolin, Sikorski, Marcin, Silenzi, Alessandro, Stan, Claudiu A., Shi, Xintian, Stern, Stephan, Sztuk-Dambietz, Jolanta, Szuba, Janusz, Tolstikova, Aleksandra, Aplin, Steven, Trebbin, Martin, Trunk, Ulrich, Vagovic, Patrik, Ve, Thomas, Weinhausen, Britta, White, Thomas A., Wrona, Krzysztof, Xu, Chen, Yefanov, Oleksandr, Zatsepin, Nadia, Awel, Salah, Zhang, Jiaguo, Perbandt, Markus, Mancuso, Adrian P., Betzel, Christian, Chapman, Henry N., Banty, Anton, Ayyer, Kartik, Bajt, Sa��a, Bar��k, Imrich, Bari, Sadia, Bielecki, Johan, Botha, Sabine, Boukhelef, Djelloul, Brehm, Wolfgang, Brockhauser, Sandor, Cheviakov, Igor, Coleman, Matthew A., Cruz-Mazo, Francisco, Danilevski, Cyril, Darmanin, Connie, Doak, R. Bruce, Domaracky, Martin, D��rner, Katerina Henrike, Du, Yang, Fangohr, Hans, Fleckenstein, Holger, Frank, Matthias, Fromme, Petra, Ga����n-Calvo, Alfonso M., Gevorkov, Yaroslav, Giewekemeyer, Klaus, Ginn, Helen Mary, Graafsma, Heinz, Graceffa, Rita, Greiffenberg, Dominic, Gumprecht, Lars, G��ttlicher, Peter, Hajdu, J��nos, Hauf, Steffen, Heymann, Michael, and Holmes, Susannah

Subjects
600: Technik, 3. Good health
Abstract

The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a ��-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.

34. X-ray screening identifies active site and allosteric inhibitors of SARS-CoV-2 main protease

Author

Günther, Sebastian, Reinke, Patrick Y. A., Fernández-García, Yaiza, Lieske, Julia, Lane, Thomas J., Ginn, Helen M., Koua, Faisal H. M., Ehrt, Christiane, Ewert, Wiebke, Oberthuer, Dominik, Yefanov, Oleksandr, Meier, Susanne, Lorenzen, Kristina, Krichel, Boris, Kopicki, Janine-Denise, Gelisio, Luca, Brehm, Wolfgang, Dunkel, Ilona, Seychell, Brandon, Gieseler, Henry, Norton-Baker, Brenna, Escudero-Pérez, Beatriz, Domaracky, Martin, Saouane, Sofiane, Tolstikova, Alexandra, White, Thomas A., Hänle, Anna, Groessler, Michael, Fleckenstein, Holger, Trost, Fabian, Galchenkova, Marina, Gevorkov, Yaroslav, Li, Chufeng, Awel, Salah, Peck, Ariana, Barthelmess, Miriam, Schluenzen, Frank, Lourdu Xavier, Paulraj, Werner, Nadine, Andaleeb, Hina, Ullah, Najeeb, Falke, Sven, Srinivasan, Vasundara, França, Bruno Alves, Schwinzer, Martin, Brognaro, Hévila, Rogers, Cromarte, Melo, Diogo, Zaitseva-Doyle, Joanna J., Knoska, Juraj, Peña-Murillo, Gisel E., Mashhour, Aida Rahmani, Hennicke, Vincent, Fischer, Pontus, Hakanpää, Johanna, Meyer, Jan, Gribbon, Philip, Ellinger, Bernhard, Kuzikov, Maria, Wolf, Markus, Beccari, Andrea R., Bourenkov, Gleb, Von Stetten, David, Pompidor, Guillaume, Bento, Isabel, Panneerselvam, Saravanan, Karpics, Ivars, Schneider, Thomas R., Garcia-Alai, Maria Marta, Niebling, Stephan, Guenther, Christian, Schmidt, Christina, Schubert, Robin, Han, Huijong, Boger, Juliane, Monteiro, Diana C. F., Zhang, Linlin, Sun, Xinyuanyuan, Pletzer-Zelgert, Jonathan, Wollenhaupt, Jan, Feiler, Christian G., Weiss, Manfred S., Schulz, Eike-Christian, Mehrabi, Pedram, Karničar, Katarina, Usenik, Aleksandra, Loboda, Jure, Tidow, Henning, Chari, Ashwin, Hilgenfeld, Rolf, Uetrecht, Charlotte, Cox, Russell, Zaliani, Andrea, Beck, Tobias, Rarey, Matthias, Günther, Stephan, Turk, Dusan, Hinrichs, Winfried, Chapman, Henry N., Pearson, Arwen R., Betzel, Christian, and Meents, Alke

Subjects
3. Good health
Abstract

Science / Science now x, eabf7945 (1-11) (2021). doi:10.1126/science.abf7945, The coronavirus disease (COVID-19) caused by SARS-CoV-2 is creating tremendous human suffering. To date, no effective drug is available to directly treat the disease. In a search for a drug against COVID-19, we have performed a high-throughput X-ray crystallographic screen of two repurposing drug libraries against the SARS-CoV-2 main protease (Mpro), which is essential for viral replication. In contrast to commonly applied X-ray fragment screening experiments with molecules of low complexity, our screen tested already approved drugs and drugs in clinical trials. From the three-dimensional protein structures, we identified 37 compounds that bind to Mpro. In subsequent cell-based viral reduction assays, one peptidomimetic and six non-peptidic compounds showed antiviral activity at non-toxic concentrations. We identified two allosteric binding sites representing attractive targets for drug development against SARS-CoV-2., Published by Assoc., Washington, DC

35. Megahertz serial crystallography

Author

Wiedorn, Max O., Oberthür, Dominik, Bean, Richard, Schubert, Robin, Werner, Nadine, Abbey, Brian, Aepfelbacher, Martin, Adriano, Luigi, Allah Gholi, Aschkan, Al-Qudami, Nasser, Andreasson, Jakob, Aplin, Steve, Awel, Salah, Ayyer, Kartik, Bajt, Saša, Barák, Imrich, Bari, Sadia, Bielecki, Johan, Botha, Sabine, Boukhelef, Djelloul, Brehm, Wolfgang, Brockhauser, Sandor, Cheviakov, Igor, Coleman, Matthew A., Cruz-Mazo, Francisco, Danilevski, Cyril, Darmanin, Connie, Doak, R. Bruce, Domaracky, Martin, Dörner, Katerina, Du, Yang, Fangohr, Hans, Fleckenstein, Holger, Frank, Matthias, Fromme, Petra, Gañán-Calvo, Alfonso M., Gevorkov, Yaroslav, Giewekemeyer, Klaus, Ginn, Helen, Graafsma, Heinz, Graceffa, Rita, Greiffenberg, Dominic, Gumprecht, Lars, Göttlicher, Peter, Hajdu, Janos, Hauf, Steffen, Heymann, Michael, Holmes, Susannah, Horke, Daniel A., Hunter, Mark S., Imlau, Siegfried, Kaukher, Alexander, Kim, Yoonhee, Klyuev, Alexander, Knoska, Juraj, Kobe, Bostjan, Kuhn, Manuela, Kupitz, Christopher, Küpper, Jochen, Lahey-Rudolph, Janine Mia, Laurus, Torsten, Le Cong, Karoline, Letrun, Romain, Xavier, P. Lourdu, Maia, Luis, Maia, Filipe R. N. C., Mariani, Valerio, Messerschmidt, Marc, Metz, Markus, Mezza, Davide, Michelat, Thomas, Mills, Grant, Monteiro, Diana, Morgan, Andrew, Mühlig, Kerstin, Munke, Anna, Münnich, Astrid, Nette, Julia, Nugent, Keith A., Nuguid, Theresa, Orville, Allen M., Pandey, Suraj, Pena, Gisel, Villanueva-Perez, Pablo, Poehlsen, Jennifer, Previtali, Gianpietro, Redecke, Lars, Riekehr, Winnie Maria, Rohde, Holger, Round, Adam, Safenreiter, Tatiana, Sarrou, Iosifina, Sato, Tokushi, Schmidt, Marius, Schmitt, Bernd, Schönherr, Robert, Schulz, Joachim, Sellberg, Jonas A., Seibert, M. Marvin, Seuring, Carolin, Shelby, Megan L., Shoeman, Robert L., Sikorski, Marcin, Silenzi, Alessandro, Stan, Claudiu A., Shi, Xintian, Stern, Stephan, Sztuk-Dambietz, Jola, Szuba, Janusz, Tolstikova, Aleksandra, Trebbin, Martin, Trunk, Ulrich, Vagovic, Patrik, Ve, Thomas, Weinhausen, Britta, White, Thomas A., Wrona, Krzysztof, Xu, Chen, Yefanov, Oleksandr, Zatsepin, Nadia, Zhang, Jiaguo, Perbandt, Markus, Mancuso, Adrian, Betzel, Christian, Chapman, Henry, and Barty, Anton

Subjects
3. Good health
Abstract

Nature Communications 9(1), 4025 (2018). doi:doi.org/10.1038/s41467-018-06156-7, The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source., Published by Nature Publishing Group, London

36. Inhibition of SARS-CoV-2 main protease by allosteric drug-binding

Author

Günther, Sebastian, Reinke, Patrick Y. A., Fernández-García, Yaiza, Lieske, Julia, Lane, Thomas J., Ginn, Helen M., Koua, Faisal H. M., Ehrt, Christiane, Ewert, Wiebke, Oberthuer, Dominik, Yefanov, Oleksandr, Meier, Susanne, Lorenzen, Kristina, Krichel, Boris, Kopicki, Janine-Denise, Gelisio, Luca, Brehm, Wolfgang, Dunkel, Ilona, Seychell, Brandon, Gieseler, Henry, Norton-Baker, Brenna, Escudero-Pérez, Beatriz, Domaracky, Martin, Saouane, Sofiane, Tolstikova, Alexandra, White, Thomas A., Hänle, Anna, Groessler, Michael, Fleckenstein, Holger, Trost, Fabian, Galchenkova, Marina, Gevorkov, Yaroslav, Li, Chufeng, Awel, Salah, Peck, Ariana, Barthelmess, Miriam, Schlünzen, Frank, Xavier, P. Lourdu, Werner, Nadine, Andaleeb, Hina, Ullah, Najeeb, Falke, Sven, Srinivasan, Vasundara, Franca, Bruno Alves, Schwinzer, Martin, Brognaro, Hévila, Rogers, Cromarte, Melo, Diogo, Zaitsev-Doyle, Jo J., Knoska, Juraj, Peña Murillo, Gisel E., Mashhour, Aida Rahmani, Guicking, Filip, Hennicke, Vincent, Fischer, Pontus, Hakanpää, Johanna, Meyer, Jan, Gribbon, Phil, Ellinger, Bernhard, Kuzikov, Maria, Wolf, Markus, Beccari, Andrea R., Bourenkov, Gleb, Stetten, David von, Pompidor, Guillaume, Bento, Isabel, Panneerselvam, Saravanan, Karpics, Ivars, Schneider, Thomas R., Garcia Alai, Maria Marta, Niebling, Stephan, Günther, Christian, Schmidt, Christina, Schubert, Robin, Han, Huijong, Boger, Juliane, Monteiro, Diana C. F., Zhang, Linlin, Sun, Xinyuanyuan, Pletzer-Zelgert, Jonathan, Wollenhaupt, Jan, Feiler, Christian G., Weiss, Manfred S., Schulz, Eike-Christian, Mehrabi, Pedram, Karničar, Katarina, Usenik, Aleksandra, Loboda, Jure, Tidow, Henning, Chari, Ashwin, Hilgenfeld, Rolf, Uetrecht, Charlotte, Cox, Russell, Zaliani, Andrea, Beck, Tobias, Rarey, Matthias, Günther, Stephan, Turk, Dusan, Hinrichs, Winfried, Chapman, Henry N., Pearson, Arwen R., Betzel, Christian, and Meents, Alke

Abstract

The coronavirus disease (COVID-19) caused by SARS-CoV-2 is creating tremendous health problems and economical challenges for mankind. To date, no effective drug is available to directly treat the disease and prevent virus spreading. In a search for a drug against COVID-19, we have performed a massive X-ray crystallographic screen of two repurposing drug libraries against the SARS-CoV-2 main protease (Mpro), which is essential for the virus replication and, thus, a potent drug target. In contrast to commonly applied X-ray fragment screening experiments with molecules of low complexity, our screen tested already approved drugs and drugs in clinical trials. From the three-dimensional protein structures, we identified 37 compounds binding to Mpro. In subsequent cell-based viral reduction assays, one peptidomimetic and five non-peptidic compounds showed antiviral activity at non-toxic concentrations. We identified two allosteric binding sites representing attractive targets for drug development against SARS-CoV-2. Competing Interest Statement

37. Megahertz serial crystallography

Author

Wiedorn, Max O., Oberthür, Dominik, Bean, Richard, Schubert, Robin, Werner, Nadine, Abbey, Brian, Aepfelbacher, Martin, Adriano, Luigi, Allahgholi, Aschkan, Al-Qudami, Nasser, Andreasson, Jakob, Aplin, Steve, Awel, Salah, Ayyer, Kartik, Bajt, Saša, Barak, Imrich, Bari, Sadia, Bielecki, Johan, Botha, Sabine, Boukhelef, Djelloul, Brehm, Wolfgang, Brockhauser, Sandor, Cheviakov, Igor, Coleman, Matthew A., Cruz-Mazo, Francisco, Danilevski, Cyril, Darmanin, Connie, Doak, R. Bruce, Domaracky, Martin, Dörner, Katerina, Du, Yang, Fangohr, Hans, Fleckenstein, Holger, Frank, Matthias, Fromme, Petra, Ganan-Calvo, Alfonso, Gevorkov, Yaroslav, Giewekemeyer, Klaus, Ginn, Helen, Graafsma, Heinz, Graceffa, Rita, Greiffenberg, Dominic, Gumprecht, Lars, Göttlicher, Peter, Hajdu, Janos, Hauf, Steffen, Heymann, Michael, Holmes, Susannah, Horke, Daniel A., Hunter, Mark S., Imlau, Siegfried, Kaukher, Alexander, Kim, Yoonhee, Klyuev, Alexander, Knoska, Juraj, Kobe, Bostjan, Kuhn, Manuela, Kupitz, Christopher, Küpper, Jochen, Lahey-Rudolph, Janine Mia, Laurus, Torsten, Le Cong, Karoline, Letrun, Romain, Xavier, P. Lourdu, Maia, Luis, Maia, Filipe R. N. C., Mariani, Valerio, Messerschmidt, Marc, Metz, Markus, Mezza, Davide, Michelat, Thomas, Mills, Grant, Monteiro, Diana, Morgan, Andrew, Mühlig, Kerstin, Munke, Anna, Münnich, Astrid, Nette, Julia, Nugent, Keith, Nuguid, Theresa, Orville, Allen, Pandey, Suraj, Pena, Gisel, Villanueva-Perez, Pablo, Poehlsen, Jennifer, Previtali, Gianpietro, Redecke, Lars, Riekehr, Winnie Maria, Rohde, Holger, Round, Adam, Safenreiter, Tatiana, Sarrou, Iosifina, Sato, Tokushi, Schmidt, Marius, Schmitt, Bernd, Schönherr, Robert, Schulz, Joachim, Sellberg, Jonas, Seibert, M. Marvin, Seuring, Carolin, Shelby, Megan, Shoeman, Robert, Sikorski, Marcin, Silenzi, Alessandro, Stan, Claudiu, Shi, Xintian, Stern, Stephan, Sztuk-Dambietz, Jola, Szuba, Janusz, Tolstikova, Aleksandra, Trebbin, Martin, Trunk, Ulrich, Vagovic, Patrik, Ve, Thomas, Weinhausen, Britta, White, Thomas A., Wrona, Krzysztof, Xu, Chen, Yefanov, Oleksandr, Zatsepin, Nadia, Zhang, Jiaguo, Perbandt, Markus, Mancuso, Adrian P., Betzel, Christian, Chapman, Henry, and Barty, Anton

Subjects
3. Good health
Abstract

Nature Communications 9(1), 4025 (2018). doi:10.1038/s41467-018-06156-7, The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reli- able structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source., Published by Nature Publishing Group, London

38. Spatio-temporal dynamics of mycosporine-like amino acids and of primary production in benthic cyanobacteria from Gossenköllesee

Author

Werner, Nadine and Werner, Nadine

Abstract

Cyanobakterien sind eines der ältesten Organismen auf der Erde und stammen aus einer Zeit in der UV-C Strahlung noch die Erdoberfläche erreichte. Auf Grund ihrer langen Evolution konnten sie somit spezielle Anpassungen entwickeln, um negative Folgen durch Sonnenstrahlung zu vermeiden. Die Synthese von Sonnenschutzverbindungen, wie mycosporinähnliche Aminosäuren (MAAs), ermöglicht es ihnen mit minimalem Zellschadenrisiko Fotosynthese betreiben zu können. Zusammenhänge zwischen Sonnenstrahlung und MAAs sind in planktonischen Cyanobakterien umfassend dokumentiert, wohingegen im benthischen Reich noch ein Informationsdefizit herrscht, insbesondere in alpinen Seen. Dieses Defizit umfasst auch den Beitrag der benthischen Cyanobakterien an der gesamten Primärproduktion, der in oligotrophen Seen dominieren soll. In dieser Arbeit wurden zeitliche und räumliche Dynamiken von MAAs sowie Bruttoprimärproduktion im Benthos des Gossenköllesees, unter Berücksichtigung des Epilithons und lose befestigter Filamente, untersucht. Sechs MAAs lagen in beiden Probetypen vor, wovon drei ähnliche Strukturen wie Shinorine, Porphyra-334 und Palythine (im Folgenden SHI, POR und PAL) aufzeigten und drei bisher noch unbekannt sind. Eines der unbekannten MAAs (Absorptionsmaximum 334 nm) wurde zusätzlich durch NMR analysiert und die chemische Struktur bestimmt und zeigte größte Similarität mit Aplysiapalythine-C. Chl-a-spezifische MAA Konzentrationen zeigen in beiden Aufnahmekategorien eine signifikante Abnahme mit der Tiefe. Aufgrund des Schattierungseffekts von Filamenten, wurden im Epilithon nur Spuren von MAAs detektiert. Die zeitliche Dynamik der MAA Konzentration im Filament zeigen Spitzenwerten Mitte August. Die MAA Komposition unterscheidet sich stark zwischen Filamenten und Epilithon, jedoch jeweils ohne signifikante räumliche oder saisonale Unterschiede. Das dominierende MAA in den Filamenten während der gesamten Saison war das unbekannte MAA mit dem Absorptionsmaximum von 334 nm (bis zu, Cyanobacteria are one of the oldest organisms on Earth and originated in a time, when UV-C radiation still reached the surface. Their long evolution led to several adaptations to avoid deleterious effects caused by exposure to solar UV radiation. Synthesis of sunscreen compounds, such as mycosporine-like amino acids (MAAs), allow them to conduct photosynthesis with minimized risk of cell damage. The interplay of solar UV radiation and MAAs is well documented in planktonic cyanobacteria, but little is known for those in the benthic realm, particularly of alpine lakes. Similarly, little is known on how much benthic cyanobacteria contribute to lake primary production, though this is expected to be high in oligotrophic lakes. Here, we assessed temporal and spatial dynamics of MAAs and gross primary production in the benthos of the alpine lake Gossenköllesee, considering the epilithon and loosely attached filamentous cyanobacteria. Six MAAs were present in both types of samples, whereby three were related to Shinorine, Porphyra-334 and Palythine (SHI, POR and PAL, hereafter) and three were unknown. One of the unknown MAAs (max. absorption at 334 nm) was analyzed via Nuclear Magnetic Resonance (NMR) and its chemical structure resulted similar to Aplysiapalythine-C. Chlorophyll a-specific MAA concentrations for epilithon and filamentous cyanobacteria showed a significant decrease with depth. Due to shading effects of the filaments on the epilithon, only traces of MAAs were found in the latter. The temporal dynamics of MAA concentrations in filaments showed a peak in mid-August. MAA composition differed greatly between filaments and epilithon, however, in either case no significant change was detected spatially or seasonally. The dominant MAA in filaments throughout the season was the Aplysiapalythine-C-like (up to 75% by weight), with marginal deviations in the deepest samples (7.7 m). In the upper three depths (0.4, 2.0 and 4.0 m), the same MAA accounted for the largest s, Nadine Werner, Zusammenfassung in deutscher Sprache, Masterarbeit Universität Innsbruck 2019

39. Spatio-temporal dynamics of mycosporine-like amino acids and of primary production in benthic cyanobacteria from Gossenköllesee

Author

Werner, Nadine and Werner, Nadine

Abstract

Cyanobakterien sind eines der ältesten Organismen auf der Erde und stammen aus einer Zeit in der UV-C Strahlung noch die Erdoberfläche erreichte. Auf Grund ihrer langen Evolution konnten sie somit spezielle Anpassungen entwickeln, um negative Folgen durch Sonnenstrahlung zu vermeiden. Die Synthese von Sonnenschutzverbindungen, wie mycosporinähnliche Aminosäuren (MAAs), ermöglicht es ihnen mit minimalem Zellschadenrisiko Fotosynthese betreiben zu können. Zusammenhänge zwischen Sonnenstrahlung und MAAs sind in planktonischen Cyanobakterien umfassend dokumentiert, wohingegen im benthischen Reich noch ein Informationsdefizit herrscht, insbesondere in alpinen Seen. Dieses Defizit umfasst auch den Beitrag der benthischen Cyanobakterien an der gesamten Primärproduktion, der in oligotrophen Seen dominieren soll. In dieser Arbeit wurden zeitliche und räumliche Dynamiken von MAAs sowie Bruttoprimärproduktion im Benthos des Gossenköllesees, unter Berücksichtigung des Epilithons und lose befestigter Filamente, untersucht. Sechs MAAs lagen in beiden Probetypen vor, wovon drei ähnliche Strukturen wie Shinorine, Porphyra-334 und Palythine (im Folgenden SHI, POR und PAL) aufzeigten und drei bisher noch unbekannt sind. Eines der unbekannten MAAs (Absorptionsmaximum 334 nm) wurde zusätzlich durch NMR analysiert und die chemische Struktur bestimmt und zeigte größte Similarität mit Aplysiapalythine-C. Chl-a-spezifische MAA Konzentrationen zeigen in beiden Aufnahmekategorien eine signifikante Abnahme mit der Tiefe. Aufgrund des Schattierungseffekts von Filamenten, wurden im Epilithon nur Spuren von MAAs detektiert. Die zeitliche Dynamik der MAA Konzentration im Filament zeigen Spitzenwerten Mitte August. Die MAA Komposition unterscheidet sich stark zwischen Filamenten und Epilithon, jedoch jeweils ohne signifikante räumliche oder saisonale Unterschiede. Das dominierende MAA in den Filamenten während der gesamten Saison war das unbekannte MAA mit dem Absorptionsmaximum von 334 nm (bis zu, Cyanobacteria are one of the oldest organisms on Earth and originated in a time, when UV-C radiation still reached the surface. Their long evolution led to several adaptations to avoid deleterious effects caused by exposure to solar UV radiation. Synthesis of sunscreen compounds, such as mycosporine-like amino acids (MAAs), allow them to conduct photosynthesis with minimized risk of cell damage. The interplay of solar UV radiation and MAAs is well documented in planktonic cyanobacteria, but little is known for those in the benthic realm, particularly of alpine lakes. Similarly, little is known on how much benthic cyanobacteria contribute to lake primary production, though this is expected to be high in oligotrophic lakes. Here, we assessed temporal and spatial dynamics of MAAs and gross primary production in the benthos of the alpine lake Gossenköllesee, considering the epilithon and loosely attached filamentous cyanobacteria. Six MAAs were present in both types of samples, whereby three were related to Shinorine, Porphyra-334 and Palythine (SHI, POR and PAL, hereafter) and three were unknown. One of the unknown MAAs (max. absorption at 334 nm) was analyzed via Nuclear Magnetic Resonance (NMR) and its chemical structure resulted similar to Aplysiapalythine-C. Chlorophyll a-specific MAA concentrations for epilithon and filamentous cyanobacteria showed a significant decrease with depth. Due to shading effects of the filaments on the epilithon, only traces of MAAs were found in the latter. The temporal dynamics of MAA concentrations in filaments showed a peak in mid-August. MAA composition differed greatly between filaments and epilithon, however, in either case no significant change was detected spatially or seasonally. The dominant MAA in filaments throughout the season was the Aplysiapalythine-C-like (up to 75% by weight), with marginal deviations in the deepest samples (7.7 m). In the upper three depths (0.4, 2.0 and 4.0 m), the same MAA accounted for the largest s, Nadine Werner, Zusammenfassung in deutscher Sprache, Masterarbeit Universität Innsbruck 2019

40. 'Kulturinstrument Radio':Zur Genealogie der medialen Katastrophe der Weimarer Republik

Author

Hagen, Wolfgang, Nitsche, Jessica, and Werner, Nadine

Subjects
Medienkultur, social media, Digitale Medien, Digitale Kultur, media theory, Kulturwissenschaften allg, Soziale Medien, digital Culture, Netzkultur, Medientheorie, Medienwissenschaften, media studies, Medien- und Kommunikationswissenschaft, Digitale Kulturen, Transdisziplinäre Studien, Medienkulturen, Neue Medien, digital Cultures, media cultures, net culture, media culture, new media
Published
2012

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