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Genetically encoded calcium indicators (GECIs) such as GCaMP are invaluable tools in neuroscience to monitor neuronal activity using optical imaging. The viral transduction of GECIs is commonly used to target expression to specific brain regions, can be conveniently used with any mouse strain of interest without the need for prior crossing with a GECI mouse line, and avoids potential hazards due to the chronic expression of GECIs during development. A key requirement for monitoring neuronal activity with an indicator is that the indicator itself minimally affects activity. Here, using common adeno-associated viral (AAV) transduction procedures, we describe spatially confined aberrant Ca2+ microwaves slowly travelling through the hippocampus following expression of GCaMP6, GCaMP7, or R-CaMP1.07 driven by the synapsin promoter with AAV-dependent gene transfer in a titre-dependent fashion. Ca2+ microwaves developed in hippocampal CA1 and CA3, but not dentate gyrus nor neocortex, were typically first observed at 4 wk after viral transduction, and persisted up to at least 8 wk. The phenomenon was robust and observed across laboratories with various experimenters and setups. Our results indicate that aberrant hippocampal Ca2+ microwaves depend on the promoter and viral titre of the GECI, density of expression, as well as the targeted brain region. We used an alternative viral transduction method of GCaMP which avoids this artefact. The results show that commonly used Ca2+-indicator AAV transduction procedures can produce artefactual Ca2+ responses. Our aim is to raise awareness in the field of these artefactual transduction-induced Ca2+ microwaves, and we provide a potential solution.

Prior research has studied preferences for identity- or person-first language among persons with an autism diagnosis. The current study differs from this previous body of research by specifically examining quantitative predictors of language preferences through a social identity theoretical approach, thereby leading to a better understanding of psychological and social factors that might underlie language use and preference within the autistic community. Australian adults with an autism diagnosis (N = 198) completed the measures of autism knowledge, internalised stigma, and autism identity to determine whether these factors predict language preference. Results indicated a stronger autism identity was associated with a preference for identity-first terms (autistic/autistic person) and finding these less offensive. Contrastingly, stigma was associated with finding identity-first language less favourable and more offensive. Person-first terms (e.g. person with autism) were not associated with any of the predictors. Together, these findings suggest decision-making around identity-first language is influenced by a strong sense of autistic identity and experiences of stigma.

Many relationships within which interpersonal transgressions transpire often involve histories of reciprocal wrongdoing, where each party has occupied both the role of victim and offender. We investigate whether past incidents of being wronged by the victim of a present transgression may dampen offenders' conciliatory sentiments for their present wrongdoing. Across four studies (combined N = 1037), we find evidence that past victimization experiences within the context of an interpersonal relationship can blur offenders' construal of their role as offender and elicit feelings of victimhood, allowing them to exonerate themselves and feel less guilt for their present wrongdoing, display less empathy for the present victim, and reduce their willingness to reconcile with the present victim. These findings highlight the importance of taking into account historical transgressions within a relationship as a determinant of relationship repair in the aftermath of present wrongdoings. [ABSTRACT FROM AUTHOR]

We investigate if and how imagining a positive future where a societal issue has been rectified can promote hope and support for collective action. Participants imagined (vs. not) a positive future related to climate change (Study 1, N = 301) and economic equality (Study 2, N = 489), two years versus several decades from now. In both studies, imagining a positive future led to support for collective action via increased hope. In Study 1, imagination increased the perceived possibility of achieving the collective goal, in Study 2 it increased its perceived desirability. Possibility was positively related to hope, in particular when imagining a near future, whereas desirability of the collective goal was positively related to hope for a distant imagined future. The research provides evidence for the power of imagination in inspiring hope and collective action, and shows the shifting meaning of hope depending on collective action context and timeframe of the imagination. [ABSTRACT FROM AUTHOR]

Pundits have speculated that the spread of conspiracies and misinformation (termed "misbeliefs") is leading to a resurgence of right‐wing, reactionary movements. However, the current empirical picture regarding the relationship between misbeliefs and collective action is mixed. We help clarify these associations by using two waves of data collected during the COVID‐19 Pandemic (in Australia, N = 519, and the United States, N = 510) and democratic elections (in New Zealand N = 603, and the United States N = 609) to examine the effects of misbeliefs on support for reactionary movements (e.g., anti‐lockdown protests, Study 1; anti‐election protests, Study 2). Results reveal that within‐person changes in misbeliefs correlate positively with support for reactionary collective action both directly (Studies 1–2) and indirectly by shaping the legitimacy of the authority (Study 1b). The relationship between misbelief and legitimacy is, however, conditioned by the stance of the authority in question: the association is positive when authorities endorse misbeliefs (Study 1a) and negative when they do not (Study 1b). Thus, the relationship between conspiracy beliefs and action hinges upon the alignment of the content of the conspiracy and the goals of the collective action. [ABSTRACT FROM AUTHOR]

The theoretical prediction of the rates of nonradiative processes in molecules is fundamental in assessing their emissive properties. In this context, global harmonic models have been widely used to simulate vibronic spectra as well as internal conversion rates and to predict photoluminescence quantum yields. However, these simplified models suffer from the limitations that are inherent to the harmonic approximation and can have a severe effect on the calculated internal conversion rates. Therefore, the development of more accurate semiclassical methods is highly desirable. Here, we introduce a procedure for the calculation of nonradiative rates in the framework of the time-dependent semi-classical Extended Thawed Gaussian Approximation (ETGA). We systematically investigate the performance of the ETGA method by comparing it to the adiabatic and vertical harmonic methods, which belong to the class of widely used global harmonic models. Its performance is tested in potentials that cannot be treated adequately by global harmonic models, beginning with Morse potentials of varying anharmonicity followed by a double well potential. The calculated radiative and nonradiative internal conversion rates are compared to reference values based on exact quantum dynamics. We find that the ETGA has the capability to predict internal conversion rates in anharmonic systems with an appreciable energy gap, whereas the global harmonic models prove to be insufficient. [ABSTRACT FROM AUTHOR]

Anecdotally, people often report feeling despair about the political status quo. We conceptualise these feelings as political despair. But what is political despair, and what are its effects? We adapt intergroup emotion theory to analyse political despair in the context of racial inequality (Studies 1 and 2) and climate change (Study 3). Three cross‐sectional studies (total N = 866) tested the measurement of political despair (relative to anger and hope), its pattern of appraisals and outcomes for conventional and radical actions along with well‐being (stress, burnout and optimism). Structural equation modelling differentiated political despair from anger and hope and found that despair is associated with evaluations that the situation is both illegitimate and intractable (unchangeable). Moreover, political despair consistently had a negative relationship with well‐being and positive relationships with conventional and radical collective action. The results suggest political despair is negatively associated with well‐being and impact people's engagement in action for social change. [ABSTRACT FROM AUTHOR]