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Stachyose (ST), a naturally occurring compound extracted from cucumbers and legumes, holds great promise as a natural therapeutic agent. In this study, the impact of ST on blood deficiency syndrome (BDS) induced by cyclophosphamide (CP) and acetylphenylhydrazine (APH) was investigated in a rat model. Subsequently, the levels of erythropoietin (EPO), granulocyte colony-stimulating factor (G-CSF), tumour necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6) were quantified in rat blood and peripheral blood cells. Furthermore, haematoxylin and eosin (HE) staining was employed to elucidate morphological alterations in the spleen tissue. To gain comprehensive insights into the therapeutic potential and underlying mechanisms of ST against BDS, we used 16S rRNA gene sequencing and serum and spleen metabolomic analyses. Our findings revealed a significant ameliorative effect of ST on BDS. Notably, at the phylum level, Firmicutes and Bacteroidota exhibited marked variations in abundance, while at the genus level, discernible changes were observed in the abundances of Lactobacillus and Bifidobacterium, among others. The therapeutic effects of ST on BDS were proposed to be due to its modulation of phenylalanine metabolism and glycine, serine and threonine metabolism and its impact on the restructuring of the gut microbiota.

Catalpol (CA), extracted from Rehmannia Radix, holds extensive promise as a natural medicinal compound. This study employed 16S rRNA gene sequencing and combined serum and spleen metabolomics to profoundly investigate the therapeutic effects of CA on blood deficiency syndrome (BDS) and the underlying mechanisms. Notably, CA exhibited effectiveness against BDS induced by cyclophosphamide (CP) and acetylphenylhydrazine (APH) in rats-CA substantially elevated levels of crucial indicators such as erythropoietin (EPO), granulocyte colony-stimulating factor (G-CSF), tumor necrosis factor-alpha (TNF-a), and interleukin-6 (IL-6). Additionally, CA could alleviate peripheral blood cytopenia. Furthermore, the analysis of 16S rRNA revealed that CA had the potential to reverse the Firmicutes/Bacteroidetes (F/B) ratio associated with BDS. Through comprehensive serum and spleen metabolomic profiling, we successfully identified 22 significant biomarkers in the serum and 23 in the spleen, respectively. Enrichment analysis underscored Glycerophospholipid metabolism and Sphingolipid metabolism as potential pathways through which CA exerts its therapeutic effects on BDS.

Bile acids are the main component of animal bile and are directly involved in the metabolic process of lipids in vivo. Taurochenodeoxycholic acid (TCDCA) is the primary biologically active substance in bile acids and has biological functions such as antioxidant, antipyretic, anti-inflammatory, and analgesic activities and improves immunity. In the present study, we assessed the impact of TCDCA on hyperlipidemia development in mouse models. Mice were fed a high-fat diet (HFD) to induce hyperlipidemia and orally administered different doses of TCDCA orally for 30 days. Then, indicators such as triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) in mice were detected. Using HE and ORO staining techniques, the morphology of the mice’s liver tissue was detected. Based on metabolomic and lipidomic analyses, we determined the mechanism of TCDCA in treating hyperlipidemia. The results showed that TCDCA had a significant ameliorating effect on dietary hyperlipidemia. In addition, it exerted therapeutic effects through glycerophospholipid metabolism.

Severe consequences can ensue from the rupture and hemorrhage of a renal artery pseudoaneurysm, necessitating immediate intervention upon detection. To date, no reports exist on the development of renal artery pseudoaneurysms as a result of renal abscesses. This document introduces the first reported case of this occurrence.

Hyperlipidaemia, which is characterized by an excess of lipids or fats in the bloodstream, is a high-risk factor and critical indicator of many metabolic diseases. This study used 16 S rRNA gene sequencing and metabolomics to determine that stachyose (ST) has a therapeutic effect and is a mechanism of hyperlipidaemia. These results show that ST significantly attenuated high-fat diet-induced weight gain and fat deposition while also adjusting the gut microbial composition. Untargeted serum metabolomics identified 12 biomarkers, which suggests that ST may function by regulating metabolic pathways. These results highlight the potential of ST in treating hyperlipidaemia and provides directions for future research including an in-depth investigation of the bioactive components, dosage, and treatment strategies of ST.

Traditional Chinese medicine (TCM) is a class of natural drugs with multiple components and significant therapeutic effects through multiple targets. It also originates from a wide range of sources containing plants, animals and minerals, and among them, plant-based Chinese medicine also includes fungi. Fungal traditional Chinese medicine is a medicinal resource with a long history and widespread application in China. Accumulating evidence confirms that polysaccharide is the main pharmacodynamic material on which fungal TCM is based. The purpose of the current systematic review is to summarize the extraction, isolation, structural identification, biological functions, quality control and medicinal and edible applications of polysaccharides from fungal TCM in the past three years. This paper will supplement and deepen the understanding and application of polysaccharides from fungal TCM, and propose some valuable insights for further research and development of drugs and functional foods.

Bear bile powder is an essential, traditional and valuable Chinese herbal medicine that clears heat, calms the liver, and improves eyesight. Early studies have shown that bear bile powder has lipid-lowering activity, but due to the scarcity of natural bear bile powder resources, it has yet to be used on a large scale. Researchers have found that tauroursodeoxycholic acid (TUDCA) is the primary characteristic bioactive substance of bear bile powder. This study aimed to investigate the therapeutic effect of TUDCA on high-fat diet (HFD)-induced hyperlipidemia. A hyperlipidemia model was established by feeding mice high-fat chow, following the intervention of different concentrations of TUDCA (25/50/100 mg/kg) orally, the hallmark biochemical indexes (total cholesterol (TC), total triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C)), histopathological examination (hematoxylin-eosin (HE) staining and oil red O (ORO) staining), and metabolomic analysis of serum and liver. The results showed that TUDCA could downregulate total TC, TG, LDL-C, upregulate HDL-C, reduce fat deposition in hepatocytes, reverse hepatocyte steatosis, and exhibit prominent lipid-lowering activity. In addition, it may play a therapeutic role by regulating glycerophospholipid metabolism.

Eleutherococcus senticosus (Rupr. & Maxim.) Maxim. leaves (ESL) have long been people’s favorite as a natural edible green vegetable, in which phenols and saponins are the main characteristic and bioactive components. This study was first carried out to comprehensively analyze the phenols and saponins in ESL, including phytochemical, qualitative, quantitative, and bioactivity analysis. The results showed that 30 compounds, including 20 phenolic compounds and 7 saponins, were identified. Twelve of them were isolated from Eleutherococcus Maxim. for the first time. In the qualitative analysis, 30 phenolic compounds and 28 saponins were accurately detected. Their characteristic cleavage processes were described by UPLC-QTOF-MS/MS. Ten representative ingredients were quantitated in 29 different regions via a 4000 QTRAP triple quadrupole tandem mass spectrometer (UPLC-QTRAP-MS/MS), and it was found that S19 (69.89 ± 1.098 mg/g) and S1 (74.28 ± 0.733 mg/g) had the highest contents of total phenols and saponins, respectively. The newly developed analysis method for the quantitative determination was validated for linearity, precision, and limits of detection and quantification, which could be applied to the quality assessment of ESL. In vitro experiment, the α-glucosidase inhibitory effect of the phenolic fraction was higher than others, indicating that the phenolic content may be related to the hypoglycemic activity. It was also suggested that ESL could be developed as a natural potential effective drug or functional food.

Radix Rehmanniae (RR, from Radix Rehmanniae (Gaertn.) DC.) is a natural medicine used in traditional Chinese medicine (TCM) since ancient times for the treatment of blood disorders. RR is steamed to get Rehmanniae Radix Praeparata (RP), which has a tonic effect on blood; the content of 5-hydromethylfurfural (5-HMF) increases more than four times after steaming. Studies have shown that 5-HMF has positive pharmacological effects on cardiovascular and hematological disorders. This study aimed to explore and verify the impact of 5-HMF on rats with chemotherapy-induced blood deficiency syndrome (BDS). Rats were given cyclophosphamide (CP) and acetophenhydrazine (APH) to induce BDS, the coefficients of some organs (liver, spleen, and kidney) were measured, and a routine blood test examined the coefficients of several peripheral blood cells. Metabolomics and network pharmacology were combined to find important biomarkers, targets, and pathways. Western blot was used to detect the expression of CYP17A1 and HSD3B1 proteins in the spleen. All these findings suggested that the 5-HMF significantly increased the number of peripheral blood cells and reversed splenomegaly in rats. In addition, 5-HMF upregulated CYP17A1 and HSD3B1 protein expression in splenic tissues. Also, 5-HMF ameliorated chemotherapy-induced BDS in rats, and its therapeutic mechanism might depend on steroid hormone biosynthesis and other pathways. It acts on blood deficiency via multiple targets and pathways, which is unique to Chinese medicine.

The purpose of this work was to illustrate the effect of processing with vinegar on saikosaponins of Bupleurum chinense DC. (BC) and the protective effects of saikosaponin A (SSA), saikosaponin b1 (SSb1), saikosaponin b2 (SSb2), and saikosaponin D (SSD) in lipopolysaccharide (LPS)-induced acute lung injury (ALI) mice. We comprehensively evaluated the anti-inflammatory effects and potential mechanisms of SSA, SSb1, SSb2, and SSD through an LPS-induced ALI model using intratracheal injection. The results showed that SSA, SSb1, SSb2, and SSD significantly decreased pulmonary edema; reduced the levels of IL-6, TNF-α, and IL-1β in serum and lung tissues; alleviated pulmonary pathological damage; and decreased the levels of the IL-6, TNF-α, and IL-1β genes and the expression of NF-κB/TLR4-related proteins. Interestingly, they were similar in structure, but SSb2 had a better anti-inflammatory effect at the same dose, according to a principal component analysis. These findings indicated that it may not have been comprehensive to only use SSA and SSD as indicators to evaluate the quality of BC, especially as the contents of SSb1 and SSb2 in vinegar-processed BC were significantly increased.

A dynamic load simulator, which can reproduce on-ground aerodynamic hinge moment of control surface, is an essential rig for the performance and stability test of an aircraft actuation system. In this paper, an observer-based backstepping adaptive control (OBAC) strategy with an improved friction model is proposed to deal with the force tracking problem of an electro-mechanical actuator under the influence of nonlinear friction and lumped disturbances. First, The LuGre friction model is improved by introducing the load effect of electrical linear load simulator (ELLS), and both dynamic and static parameters are identified with experimental data. Then, the ELLS system is divided into a loading subsystem and actuation subsystem for backstepping controller deign. The estimation of the position disturbance is obtained using an extended state observer and used for feedforward compensation for the loading subsystem. To reject the disturbance of friction parameter uncertainties for actuation subsystem, a friction scale factor with a reasonable adaptive updating law is introduced during the friction compensation process. Finally, the stability of the whole closed-loop system is demonstrated using a Lyapunov-based method, and experiments are performed to validate the effectiveness of the developed algorithm.

With the development of more/all electric aircraft, replacement of the traditional hydraulic servo actuator (HSA) with an electromechanical actuator (EMA) is becoming increasingly attractive in the aerospace field. This paper takes an EMA for a trimmable horizontal stabilizer as an example and focuses on how to establish a system model with an appropriate level of complexity to support the model-based system engineering (MBSE) approach. To distinguish the nonlinear effects that dominate the required system performance, an incremental approach is proposed to progressively introduce individual nonlinear effects into models with different complexity levels. Considering the special design and working principle of the mechanical power transmission function for this actuator, the nonlinear dynamics, including friction and backlash from the no-back mechanism, and the nonlinear compliance effect from the mechanical load path are mainly taken into consideration. The modelling principles for each effect are addressed in detail and the parameter identification method is utilized to model these nonlinear effects realistically. Finally, the responses from each model and experimental results are compared to analyze and verify how each individual nonlinearity affects the system’s performance.

Background Most of the existing studies on the influencing factors of pulmonary tuberculosis incidence are based on temporal or spatial regression models, and the results are limited. Objective To explore the temporal and spatial heterogeneity of pulmonary tuberculosis in China, and to analyze the temporal and spatial correlations between the incidence of pulmonary tuberculosis and meteorological and air quality factors, offering a scientific reference for the development of measures containing tuberculosis. Methods Monthly statistical data of pulmonary tuberculosis in China from 2016 to 2018 were collected. After being tested with multicollinearity and spatial-autocorrelation between incidence of pulmonary tuberculosis and meteorological and air quality factors, the incidence of pulmonary tuberculosis was used as the dependent variable, and meteorological and air quality factors as independent variables to construct OLS, GWR and GTWR models, respectively. Then the goodness of the three models was evaluated, and the optimal model was selected to describe the incidence of pulmonary tuberculosis. Kernel density plot and spatio-temporal graph were used to describe the spatio-temporal specificity of the fitting coefficients of each variable. Results The overall incidence of pulmonary tuberculosis in China during 2016-2018 decreased annually, with clustered spatial distribution. The GTWR model had higher R2 value and lower AICc value compared to other two models, indicating that it had better performance in explaining the influence of meteorological and air quality factors on the incidence of pulmonary tuberculosis. The kernel density plot of each variable showed that the increase of wind speed was associated with decreased pulmonary tuberculosis incidence in most cities. But the increase of humidity and air pollutant concentration was associated with increased incidence of pulmonary tuberculosis, and the strength of association varied across cities. Conclusion Meteorological and air quality factors may significantly influence the incidence of pulmonary tuberculosis, and the influence had spatio-temporal specificity. So prevention methods for pulmonary tuberculosis should be developed according to region-specific factors influencing the disease.