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[Objectives] This study was conducted to screen exit suitable agents for controlling tobacco mosaic virus disease and the best control period in Zhangzhou tobacco area, providing a theoretical basis for the control of virus diseases, thereby improving the quality of flue-cured tobacco and the income of tobacco fanners. [Methods] The effects on tobacco mosaic virus disease under the interaction between different agents and different application periods were investigated. The incidence of tobacco mosaic virus disease was investigated, and iLs control effect was analyzed. [ Results] Different agents and different abdication periods hail different control effects on tobacco mosaic virus disease. The incidence of tobacco mosaic virus disease: At 30 and 45 d after transplanting, the incidences of A2,B1, treatment were the lowest, at 0.85% , 1.71%, respectively; and at 60 d after transplanting, the incidence of A3B, treatment was the lowest, only 10.68%. The control effect; At 30 and 45 d after transplanting, A2B1 treatment hail better control effects, reaching 79.39% and 73.06% , respectively. [Conclusions] 3% hypersensitive jiroteiii sprayed at 1 d before transplanting and 7 and 15 d after transplanting achieved the best effect, followed by 10% ningnanmycin sprayed at 1 d before transplanting and 7 and 15 d after transplanting. In tobacco production, it is recommended to apply 1 000 times dilution of 3% supersensitive protein micro-granules for three times (at 1 d before transplanting and 7 and 15 d after transplanting) , which can effectively prevent tobacco mosaic virus disease. [ABSTRACT FROM AUTHOR]