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Xiaomin Zhang,1,* Jun Huang,2,* Fan Weng,3 Yanting Wen,3 Xiaoxia Wang,2 Junrong Jiang,4 Yumei Xue,4 Kun Li1 1School of Nursing, Sun Yat-Sen University, Guangzhou, People’s Republic of China; 2Department of Geriatrics, Guangdong Provincial Geriatrics Institute, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, People’s Republic of China; 3Yuexiu District Center for Disease Control and Prevention, Guangzhou, People’s Republic of China; 4Guangdong Cardiovascular Institute, Guangdong Provincial People’s Hospital, Guangzhou, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yumei Xue, Guangdong Cardiovascular Institute, Guangdong Provincial People’s Hospital, No. 106 Zhong Shan Second Road, Guangzhou, 510080, People’s Republic of China, Tel +8613570082363, Email 1295439278@qq.com Kun Li, School of Nursing, Sun Yat-sen University, No. 74 Zhong Shan Second Road, Guangzhou, 510080, People’s Republic of China, Tel +8613822206519, Email likun22@mail.sysu.edu.cnBackground: Investigating adherence to the Atrial Fibrillation Better Care (ABC) pathway management and identifying gaps between the real world and established guidelines can lead to better integrated management of AF. Current data on adherence to ABC pathway management among community elderly patients with atrial fibrillation (AF) in Chinese communities is limited.Aim: To investigate the adherence to ABC pathway management among community elderly patients with AF in China.Methods: In this cross-sectional study, data were collected from the 2020 National Basic Public Health Service Program database that includes health examination information for all residents > 65 years of age in Yuexiu, Guangzhou, Guangdong Province. Demographic and clinical characteristics data from 197 community AF patients were obtained.Results: Among the 197 AF patients, 103 (52.3%) were male, 11

Yan Du,1,* Xueru Duan,1,* Murui Zheng,2,* Wenjing Zhao,3,* Jun Huang,4 Lixian Lao,5 Fan Weng,6 Dan-Er Lin,7 Zhenxiao Yang,1 Haiyi Li,1 Xudong Liu,1 Hai Deng5 1Department of Epidemiology, School of Public Health, Sun Yat-sen University, Guangzhou, People’s Republic of China; 2Guangzhou Center for Disease Control and Prevention, Guangzhou, People’s Republic of China; 3Department of Public Health, Faculty of Medicine, Hokkaido University, Sapporo, Japan; 4Department of Geriatrics, Institute of Geriatrics, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Science, Guangzhou, People’s Republic of China; 5Department of Cardiology, Guangdong Cardiovascular Institute, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Science, Guangzhou, People’s Republic of China; 6Guangzhou Yuexiu District Center for Disease Control and Prevention, Guangzhou, People’s Republic of China; 7Guangzhou Baiyun District Center for Disease Control and Prevention, Guangzhou, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xudong Liu; Hai Deng Tel +86-20-87330619; +86-20-83827812-10263Email liuxudong@mail.sysu.edu.cn; doctordh@hotmail.comObjective: The effect of dietary factors on OSA remains unclear. This study was to investigate the association of obstructive sleep apnea (OSA) with individual and overall effects of eating habits among adult Chinese.Methods: A total of 9733 participants were selected from the baseline survey of the Guangzhou Heart Study. OSA was ascertained by Berlin Questionnaire. Food consumption was collected using a food frequency questionnaire, and eating patterns were extracted by principal component analysis. Odds ratios (ORs) with 95% confidence intervals (95% CIs) were calculated by the logistic regression model.Results: After adjustment for confounders, fried food intake with ≥ 1/week was associated with an increased risk of OSA (OR: 1.7

Chio Weng Fan., Thesis (M.Phil.)--Chinese University of Hong Kong, 2002., Includes bibliographical references (leaves 131-149)., s in English and Chinese., Acknowledgments --- p.i, p.ii, Contents --- p.iv, List of tables --- p.ix, List of figures --- p.x, List of abbreviation --- p.xii, Chapter Chapter 1 --- Introduction --- p.1, Chapter 1.1 --- Biology of Staphylococci --- p.1, Chapter 1.1.1 --- Taxonomy --- p.1, Chapter 1.1.2 --- Characteristics of S. aureus --- p.2, Chapter 1.2 --- Methicillin-resistant Staphylococcus aureus --- p.1, Chapter 1.2.1 --- Description of MRSA --- p.7, Chapter 1.2.2 --- Antibiotic Resistance of MRSA --- p.7, Chapter 1.2.2.1 --- Resistance to β-lactam Antibiotics --- p.8, Chapter 1.2.2.1.1 --- Production of β-lactamase --- p.8, Chapter 1.2.2.1.2 --- Penicillin-binding Protein PBP2a --- p.8, Chapter 1.2.2.1.3 --- Borderline Methicillin Resistance --- p.11, Chapter 1.2.2.2 --- Resistance to Antibiotics other than β-lactams --- p.11, Chapter 1.2.3 --- Epidemiology of MRSA --- p.15, Chapter 1.2.3.1 --- Clinical Significance of MRSA --- p.15, Chapter 1.2.3.1.1 --- Evolution of MRSA --- p.16, Chapter 1.2.3.1.2 --- Epidemiology of MRSA Worldwide --- p.17, Chapter 1.2.3.2 --- MRSA in Hong Kong --- p.21, Chapter 1.3 --- Strain Typing for MRSA --- p.26, Chapter 1.3.1 --- Phenotypic Methods --- p.27, Chapter 1.3.1.1 --- Antibiotic Susceptibility Test --- p.27, Chapter 1.3.1.2 --- Bacteriophage Typing --- p.28, Chapter 1.3.1.3 --- Multilocus Enzyme Electrophoresis --- p.29, Chapter 1.3.2 --- Genotypic Methods --- p.30, Chapter 1.3.2.1 --- Analysis of Chromosomal DNA --- p.30, Chapter 1.3.2.1.1 --- Pulsed-field Gel Electrophoresis (PFGE) --- p.30, Chapter 1.3.2.1.2 --- Amplified Fragment Length Polymorphism (AFLP) --- p.31, Chapter 1.3.2.2 --- Analysis of Gene Polymorphism --- p.32, Chapter 1.3.2.2.1 --- PCR-Restriction Length Polymorphism (PCR-RFLP) --- p.32, Chapter 1.3.2.2.2 --- Random Amplification of Polymorphic DNA (RAPD) --- p.33, Chapter 1.3.2.2.3 --- Nucleotide Sequence Typing --- p.33, Chapter 1.3.2.2.3.1 --- A Typing --- p.33, Chapter 1.3.2.2.3.2 --- Multilocus Sequence Typing --- p.33, Chapter 1.3.2.3 --- Hybridization by Southern Blotting --- p.34, Chapter 1.3.2.3.1 --- MecA/Tn544 Probe Typing --- p.34, Chapter 1.3.2.3.2 --- Binary Typing --- p.34, Chapter 1.3.2.4 --- Analysis of Plasmid DNA --- p.35, Chapter 1.4 --- Objectives of the Project --- p.37, Chapter Chapter 2 --- Materials & Methods --- p.38, Chapter 2.1 --- Bacterial Isolates & Culture Conditions --- p.38, Chapter 2.1.1 --- Study Period & Sample Sources --- p.38, Chapter 2.1.2 --- Selection of Bacterial Isolates --- p.38, Chapter 2.1.3 --- Reference Strains --- p.38, Chapter 2.1.4 --- Culture & Storage Conditions --- p.39, Chapter 2.1.5 --- Identification of MRSA --- p.39, Chapter 2.2 --- Antibiotic Susceptibility Testing --- p.40, Chapter 2.3 --- PCR for MecA Gene --- p.43, Chapter 2.3.1 --- DNA Preparation and Primer Design for mecA Gene --- p.43, Chapter 2.3.2 --- PCR Amplification of mecA Gene --- p.45, Chapter 2.3.2.1 --- Master Mix Preparation --- p.45, Chapter 2.3.2.2 --- Polymerase Chain Reaction --- p.45, Chapter 2.3.2.3 --- Analysis of PCR Products by Agarose Gel Electrophoresis --- p.45, Chapter 2.3.2.4 --- Precautions to Avoid Cross-contamination --- p.46, Chapter 2.4 --- Pulsed-field Gel Electrophoresis (PFGE) --- p.47, Chapter 2.4.1 --- PFGE Protocol --- p.47, Chapter 2.4.1.1 --- DNA Preparation for PFGE --- p.47, Chapter 2.4.1.2 --- Restriction Enzyme Digestion --- p.48, Chapter 2.4.1.3 --- Pulsed-field Gel Electrophoresis --- p.48, Chapter 2.4.1.4 --- Standards & Markers for PFGE --- p.48, Chapter 2.4.2 --- Optimization of PFGE --- p.49, Chapter 2.4.3 --- Computer Analysis of PFGE Patterns --- p.49, Chapter 2.5 --- Amplified Fragment Length Polymorphism (AFLP) --- p.52, Chapter 2.5.1 --- Amplified Fragment Length Polymorphism Protocol --- p.52, Chapter 2.5.1.1 --- DNA Preparation --- p.52, Chapter 2.5.1.2 --- Enzyme Digestion & Ligation --- p.53, Chapter 2.5.1.3 --- PCR for AFLP --- p.53, Chapter 2.5.1.4 --- Gel Electrophoresis for AFLP --- p.54, Chapter 2.5.1.5 --- Standards & Markers for AFLP --- p.55, Chapter 2.5.2 --- Computer Analysis for AFLP Patterns --- p.55, Chapter 2.6 --- Phage Typing --- p.58, Chapter 2.6.1 --- Phages & Propagating Strains --- p.58, Chapter 2.6.2 --- Phage Typing Protocol --- p.58, Chapter 2.6.3 --- Data Analysis and Results Interpretation for Phage Tying --- p.62, Chapter 2.7 --- Other Typing Methods --- p.63, Chapter 2.7.1 --- PCR Restriction Fragment Length Polymorphism for the coa gene --- p.63, Chapter 2.7.1.1 --- Primer Design --- p.63, Chapter 2.7.1.2 --- DNA Preparation --- p.63, Chapter 2.7.1.3 --- Optimization of PCR --- p.63, Chapter 2.7.1.3.1 --- PCR Amplification --- p.64, Chapter 2.7.1.3.2 --- Restriction Enzyme Digestion --- p.64, Chapter 2.7.2 --- Multilocus Sequence Typing --- p.64, Chapter 2.7.2.1 --- Preparation of Chromosomal DNA --- p.65, Chapter 2.7.2.2 --- PCR for MLST Gene --- p.65, Chapter 2.7.2.3 --- Purification of DNA for Sequencing --- p.67, Chapter 2.7.2.4 --- PCR for Sequencing --- p.67, Chapter 2.7.2.5 --- Sequencing by Automatic DNA Analyzer & Data Analysis --- p.68, Chapter Chapter 3 --- Results --- p.69, Chapter 3.1 --- Bacterial Isolates --- p.69, Chapter 3.2 --- Antibiotic Susceptibility Testing --- p.72, Chapter 3.3 --- PCR for MecA Gene --- p.78, Chapter 3.4 --- Pulsed-field Gel Electrophoresis --- p.80, Chapter 3.4.1 --- Optimization of PFGE --- p.80, Chapter 3.4.2 --- Analysis of PFGE Profiles --- p.83, Chapter 3.5 --- Amplified Fragment Length Polymorphism --- p.95, Chapter 3.6 --- Phage Typing --- p.102, Chapter 3.7 --- Other Typing Methods --- p.109, Chapter 3.7.1 --- PCR Restriction Fragment Length Polymorphism for the Coa Gene --- p.109, Chapter 3.7.1.1 --- Optimization of PCR conditions for the Coa Gene --- p.109, Chapter 3.7.1.2 --- Analysis of MRSA by PCR-RFLP of the coa Gene --- p.109, Chapter 3.7.2 --- Multilocus Sequence Typing --- p.115, Chapter Chapter 4 --- Discussion --- p.116, Chapter 4.1 --- Evaluation of Typing methods for MRSA --- p.116, Chapter 4.1.1 --- Antibiotic Susceptibility Testing --- p.116, Chapter 4.1.2 --- Phage Typing --- p.117, Chapter 4.1.3 --- Pulsed-field Gel Electrophoresis --- p.118, Chapter 4.1.4 --- Amplified Fragment Length Polymorphism --- p.119, Chapter 4.1.5 --- PCR-Restriction Fragment Length Polymorphism for the Coa Gene --- p.120, Chapter 4.1.6 --- Multilocus Sequence Typing --- p.121, Chapter 4.1.7 --- Conclusion for Method Evaluation --- p.122, Chapter 4.2 --- "Analysis of Results of Antibiotic Susceptibility Test, Phage Typing, PFGE, AFLP, PCR-RFLP (Coa) & MLST" --- p.124, Chapter 4.2.1 --- Correlation between Methods --- p.124, Chapter 4.2.2 --- Clinical Correlation --- p.125, Chapter 4.2.3 --- Correlation between MRSA Isolates at PWH and Reference Strains --- p.128, Chapter 4.3 --- Future Research --- p.129, Chapter 4.4 --- Conclusion --- p.130, Reference List --- p.131, Appendix I Reagent & Material Lists for Methods --- p.150, Appendix II Buffers & Media --- p.156, Appendix III Coa Gene Sequences of Isolates from PWH and Reference Strains --- p.158, Appendix IV Unique antibiotic resistance profiles --- p.165, "Appendix V Details of MRSA iolates selected for AFLP, Phage typing and MLST" --- p.166, http://library.cuhk.edu.hk/record=b5891350, Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)