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2. Cytoplasmic p21 is a potential predictor for cisplatin sensitivity in ovarian cancer

Author

Liu Ronghua, Han Zhiqiang, Liao Shujie, Weng Yanjie, Weng Danhui, Fang Yong, Li Kezhen, Xu Hongbin, Chen Pingbo, Ji Teng, Li Xiao, Ma Quanfu, Xia Xi, Zhu Tao, Wang Shixuan, Xu Gang, Meng Li, Zhou Jianfeng, and Ma Ding

Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract

Abstract Background P21(WAF1/Cip1) binds to cyclin-dependent kinase complexes and inhibits their activities. It was originally described as an inhibitor of cancer cell proliferation. However, many recent studies have shown that p21 promotes tumor progression when accumulated in the cell cytoplasm. So far, little is known about the correlation between cytoplasmic p21 and drug resistance. This study was aimed to investigate the role of p21 in the cisplatin resistance of ovarian cancer. Methods RT-PCR, western blot and immunofluorescence were used to detect p21 expression and location in cisplatin-resistant ovarian cancer cell line C13* and its parental line OV2008. Regulation of cytoplasmic p21 was performed through transfection of p21 siRNA, Akt2 shRNA and Akt2 constitutively active vector in the two cell lines; their effects on cisplatin-induced apoptosis were evaluated by flow cytometry. Tumor tissue sections of clinical samples were analyzed by immunohistochemistry. Results p21 predominantly localizes to the cytoplasm in C13* compared to OV2008. Persistent exposure to low dose cisplatin in OV2008 leads to p21 translocation from nuclear to cytoplasm, while it had not impact on p21 localization in C13*. Knockdown of cytoplasmic p21 by p21 siRNA transfection in C13* notably increased cisplatin-induced apoptosis through activation of caspase 3. Inhibition of p21 translocation into the cytoplasm by transfection of Akt2 shRNA into C13* cells significantly increased cisplatin-induced apoptosis, while induction of p21 translocation into the cytoplasm by transfection of constitutively active Akt2 in OV2008 enhanced the resistance to cisplatin. Immunohistochemical analysis of clinical ovarian tumor tissues demonstrated that cytoplasmic p21 was negatively correlated with the response to cisplatin based treatment. Conclusions Cytoplasmic p21 is a novel biomarker of cisplatin resistance and it may represent a potential therapeutic target for ovarian tumors that are refractory to conventional treatment.

Published
2011
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3. Adjuvant chemotherapy versus adjuvant concurrent chemoradiotherapy after radical surgery for early-stage cervical cancer: a randomized, non-inferiority, multicenter trial

Author

Weng, Danhui, Xiong, Huihua, Zhu, Changkun, Wan, Xiaoyun, Chen, Yaxia, Wang, Xinyu, Zhang, Youzhong, Jiang, Jie, Zhang, Xi, Gao, Qinglei, Chen, Gang, Xing, Hui, Wang, Changyu, Li, Kezhen, Chen, Yaheng, Mao, Yuyan, Hu, Dongxiao, Pan, Zimin, Chen, Qingqin, Cui, Baoxia, Song, Kun, Yi, Cunjian, Peng, Guangcai, Han, Xiaobing, An, Ruifang, Fan, Liangsheng, Wang, Wei, Xiong, Tingchuan, Chen, Yile, Tang, Zhenzi, Li, Lin, Yang, Xingsheng, Cheng, Xiaodong, Lu, Weiguo, Wang, Hui, Kong, Beihua, Xie, Xing, and Ma, Ding

Published
2023
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4. Chemotherapy initiation with single-course methotrexate alone or combined with dactinomycin versus multi-course methotrexate for low-risk gestational trophoblastic neoplasia: a multi-centric randomized clinical trial

Author

Chen, Lili, Xi, Ling, Jiang, Jie, Yin, Rutie, Qu, Pengpeng, Li, Xiuqin, Wan, Xiaoyun, Chen, Yaxia, Hu, Dongxiao, Mao, Yuyan, Pan, Zimin, Cheng, Xiaodong, Wang, Xinyu, Li, Qingli, Weng, Danhui, Zhang, Xi, Zhang, Hong, Ping, Quanhong, Liu, Xiaomei, Xie, Xing, Kong, Beihua, Ma, Ding, and Lu, Weiguo

Published
2022
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11. Risk Assessment and Triage Strategy of Cervical Cancer Primary Screening On HPV Integration Status

Author

Tian, Xun, primary, Weng, Danhui MD; PhD, additional, Chen, Ye, additional, Wang, Yi, additional, Li, Xiao, additional, Wang, Xin, additional, Cao, Chen, additional, Gong, Danni, additional, Zeng, Zhen, additional, Wu, Qiongyan, additional, Wang, Xueqian, additional, Wu, Peng, additional, Fan, Lu, additional, Zhang, Qinghua, additional, Wang, Hui, additional, Hu, Zheng, additional, Cheng, Xiaodong, additional, and Ma, Ding, additional

Published
2023
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13. Adjuvant chemotherapy versus adjuvant concurrent chemoradiotherapy after radical surgery for early-stage cervical cancer: a randomized, non-inferiority, multicenter trial

Author

Weng, Danhui, primary, Xiong, Huihua, additional, Zhu, Changkun, additional, Wan, Xiaoyun, additional, Chen, Yaxia, additional, Wang, Xinyu, additional, Zhang, Youzhong, additional, Jiang, Jie, additional, Zhang, Xi, additional, Gao, Qinglei, additional, Chen, Gang, additional, Xing, Hui, additional, Wang, Changyu, additional, Li, Kezhen, additional, Chen, Yaheng, additional, Mao, Yuyan, additional, Hu, Dongxiao, additional, Pan, Zimin, additional, Chen, Qingqin, additional, Cui, Baoxia, additional, Song, Kun, additional, Yi, Cunjian, additional, Peng, Guangcai, additional, Han, Xiaobing, additional, An, Ruifang, additional, Fan, Liangsheng, additional, Wang, Wei, additional, Xiong, Tingchuan, additional, Chen, Yile, additional, Tang, Zhenzi, additional, Li, Lin, additional, Yang, Xingsheng, additional, Cheng, Xiaodong, additional, Lu, Weiguo, additional, Wang, Hui, additional, Kong, Beihua, additional, Xie, Xing, additional, and Ma, Ding, additional

Published
2022
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16. Effectiveness and safety of niraparib as neoadjuvant therapy in advanced ovarian cancer with homologous recombination deficiency: NANT study protocol for a prospective, multicenter, exploratory, phase 2, single-arm study (041)

Author

Yu, Yang, primary, Zhang, Wei, additional, Liu, Ronghua, additional, Shan, Wanying, additional, Li, Huayi, additional, Liu, Jiahao, additional, Xia, Bairong, additional, He, Shanyang, additional, Xia, Yu, additional, Wang, Siyuan, additional, Fang, Tian, additional, Wang, Zhi, additional, Liu, Rong, additional, Weng, Danhui, additional, Chen, Youguo, additional, Song, Kun, additional, Yao, Shuzhong, additional, Li, Jundong, additional, Wang, Li, additional, Li, Qingshui, additional, Yu, Jinjin, additional, Ma, Ding, additional, Hong, Li, additional, Huang, Yi, additional, and Gao, Qinglei, additional

Published
2022
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25. Chemotherapy initiation with single-course methotrexate alone or combined with dactinomycin versus multi-course methotrexate for low-risk gestational trophoblastic neoplasia: a multi-centric randomized clinical trial

Author

Chen, Lili, primary, Xi, Ling, additional, Jiang, Jie, additional, Yin, Rutie, additional, Qu, Pengpeng, additional, Li, Xiuqin, additional, Wan, Xiaoyun, additional, Chen, Yaxia, additional, Hu, Dongxiao, additional, Mao, Yuyan, additional, Pan, Zimin, additional, Cheng, Xiaodong, additional, Wang, Xinyu, additional, Li, Qingli, additional, Weng, Danhui, additional, Zhang, Xi, additional, Zhang, Hong, additional, Ping, Quanhong, additional, Liu, Xiaomei, additional, Xie, Xing, additional, Kong, Beihua, additional, Ma, Ding, additional, and Lu, Weiguo, additional

Published
2021
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32. MCP-1/CCR-2 axis in adipocytes and cancer cell respectively facilitates ovarian cancer peritoneal metastasis

Author

Sun, Chaoyang, primary, Li, Xi, additional, Guo, Ensong, additional, Li, Na, additional, Zhou, Bo, additional, Lu, Hao, additional, Huang, Jia, additional, Xia, Meng, additional, Shan, Wanying, additional, Wang, Beibei, additional, Li, Kezhen, additional, Weng, Danhui, additional, Xu, Xiaoyan, additional, Gao, Qinglei, additional, Wang, Shixuan, additional, Hu, Junbo, additional, Lu, Yiling, additional, Mills, Gordon B., additional, and Chen, Gang, additional

Published
2019
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34. Double-Dose Adenovirus-Mediated Adjuvant Gene Therapy Improves Liver Transplantation Outcomes in Patients with Advanced Hepatocellular Carcinoma

Author

Zhu, Ruidong, primary, Weng, Danhui, additional, Lu, Shichun, additional, Lin, Dongdong, additional, Wang, Menglong, additional, Chen, Dongdong, additional, Lv, Jun, additional, Li, Hongjun, additional, Lv, Fudong, additional, Xi, Ling, additional, Zhou, Jianfeng, additional, Ma, Ding, additional, and Li, Ning, additional

Published
2018
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35. Clinicopathological Significance of VEGF-C, VEGFR-3 and Cyclooxygenase-2 in Early-Stage Cervical Cancer

Author

Shi, Xiaoyan, Xi, Ling, Weng, Danhui, Chen, Gang, Song, Xiaohong, Wu, Peng, Wang, Beibei, Wei, Juncheng, Wang, Shixuan, Zhou, Jianfeng, and Ma, Ding

Subjects
lymph node metastasis, cyclooxygenase-2, cervical cancer, vascular endothelial growth factor-C, vascular endothelial growth factor receptor-3, Article, lymphagiogenesis
Abstract

To investigate the roles of VEGF-C, VEGFR-3 and cyclooxygenase-2 (COX-2) in tumor progression and lymph node metastasis. The expression of VEGF-C, VEGFR-3 and COX-2 were examined in 93 cases of surgical speciments of cervical diseases by immunohistochemical staining. The correlation between expression of these factors and tumor aggressiveness was evaluated. The expression levels of VEGF-C and COX-2 were much higher in cervical cancer than in cervical intraepithelial neoplasia (CIN) and in chronic cervicitis. VEGF-C expression correlated with lymph node metastases (P

Published
2008

37. Proteomics-based identification of VDAC1 as a tumor promoter in cervical carcinoma

Author

Zhang, Changlin, primary, Ding, Wencheng, additional, Liu, Yuan, additional, Hu, Zheng, additional, Zhu, Da, additional, Wang, Xiaoli, additional, Yu, Lan, additional, Wang, Liming, additional, Shen, Hui, additional, Zhang, Weican, additional, Ren, Ci, additional, Li, Kezhen, additional, Weng, Danhui, additional, Deng, Wuguo, additional, Ma, Ding, additional, and Wang, Hui, additional

Published
2016
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38. Cisplatin-induced CCL5 secretion from CAFs promotes cisplatin-resistance in ovarian cancer via regulation of the STAT3 and PI3K/Akt signaling pathways

Author

ZHOU, BO, primary, SUN, CHAOYANG, additional, LI, NA, additional, SHAN, WANYING, additional, LU, HAO, additional, GUO, LILI, additional, GUO, ENSONG, additional, XIA, MENG, additional, WENG, DANHUI, additional, MENG, LI, additional, HU, JUNBO, additional, MA, DING, additional, and CHEN, GANG, additional

Published
2016
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40. Adenovirus-mediated delivery of herpes simplex virus thymidine kinase administration improves outcome of recurrent high-grade glioma

Author

Ji, Nan, primary, Weng, Danhui, additional, Liu, Cang, additional, Gu, Zheng, additional, Chen, Shizhang, additional, Guo, Ying, additional, Fan, Zhong, additional, Wang, Xiao, additional, Chen, Jianfei, additional, Zhao, Yanyan, additional, Zhou, Jianfeng, additional, Wang, Jisheng, additional, Ma, Ding, additional, and Li, Ning, additional

Published
2015
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41. Reversal of multidrug resistance and inhibition of phosphorylation of AKT in human ovarian cancer cell line by wild-type PTEN gene

Author

Xing Hui, Weng Danhui (翁丹卉), Ma Ding, Wu Huijuan (吴 娟), and LU Yunping

Subjects
Biomedical Engineering, Biology, Biochemistry, Flow cytometry, Biomaterials, Cell Line, Tumor, Genetics, medicine, PTEN, Humans, RNA, Messenger, Phosphorylation, Protein kinase B, Earth-Surface Processes, Cisplatin, Ovarian Neoplasms, medicine.diagnostic_test, PTEN Phosphohydrolase, Transfection, Molecular biology, Drug Resistance, Multiple, Blot, Apoptosis, Drug Resistance, Neoplasm, Cancer research, biology.protein, Female, Proto-Oncogene Proteins c-akt, medicine.drug
Abstract

The reversing effect of wild-type PTEN gene on resistance of C13K cells to cisplatin and its inhibitory effect on the phosphorylation of protein kinase B (AKT) were studied. The expression of PTEN mRNA and protein in OV2008 cells and C13K cells were semi-quantitatively detected by using RT-PCR and Western blotting. Recombinant eukaryotic expression plasmid containing human wild-type PTEN gene was transfected into C13K cells by lipofectamine2000. The expression of PTEN mRNA was monitored by RT-PCR and the expression of PTEN, Akt, p-Akt protein were analyzed by Western blotting in PTEN-transfected and non-transfected C13K cells. Proliferation and chemosensitivity of cells to DDP were measured by MTT, and cell apoptosis was detected by flow cytometry after treatment with cisplatin. The expression of PTEN mRNA and protein in OV2008 cells were significantly higher than those in C13K cells. After transfection with PTEN gene for 48 h, the expression of PTEN mRNA and protein in C13K cells were 2.04 +/- 0.10, 0.94 +/- 0.04 respectively and the expression of p-Akt protein (0.94 +/- 0.07) was lower than those in control groups (1.68 +/- 0.14, 1.66 +/- 0.10) (P0.05). The IC(50) of DDP to C13K cells transfected with PTEN (7.2 +/- 0.3 micromol/L) was obviously lower than those of empty-vector transfected cells and non-transfected cells (12.7 +/- 0.4 micromol/l, 13.0 +/- 0.3 micromol/L) (P0.05). The apopototis ratio of wild-type PTEN-transfected, empty vector transfected and non-transfected C13K cells were (41.65 +/- 0.87)%, (18.61 +/- 0.70)% and (15.28 +/- 0.80)% respectively, and the difference was statistically significant (P0.05). PTEN gene plays an important role in ovarian cancer multidrug resistance. Transfection of PTEN could increase the expression of PTEN and restore drug sensitivity to cisplatin in human ovarian cancer cell line C13K with multidrug-resistance by decreasing the expression of p-Akt.

Published
2007

47. Cytoplasmic p21 is a potential predictor for cisplatin sensitivity in ovarian cancer

Author

Xia, Xi, primary, Ma, Quanfu, additional, Li, Xiao, additional, Ji, Teng, additional, Chen, Pingbo, additional, Xu, Hongbin, additional, Li, Kezhen, additional, Fang, Yong, additional, Weng, Danhui, additional, Weng, Yanjie, additional, Liao, Shujie, additional, Han, Zhiqiang, additional, Liu, Ronghua, additional, Zhu, Tao, additional, Wang, Shixuan, additional, Xu, Gang, additional, Meng, Li, additional, Zhou, Jianfeng, additional, and Ma, Ding, additional

Published
2011
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49. Adjuvant Adenovirus-Mediated Delivery of Herpes Simplex Virus Thymidine Kinase Administration Improves Outcome of Liver Transplantation in Patients with Advanced Hepatocellular Carcinoma

Author

Li, Ning, primary, Zhou, Jianfeng, additional, Weng, Danhui, additional, Zhang, Chenghua, additional, Li, Lixin, additional, Wang, Beibei, additional, Song, Yang, additional, He, Qiang, additional, Lin, Dongdong, additional, Chen, Dazhi, additional, Chen, Gang, additional, Gao, Qinglei, additional, Wang, Shixuan, additional, Xu, Gang, additional, Meng, Li, additional, Lu, YunPing, additional, and Ma, Ding, additional

Published
2007
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