1. Synthesis, interaction with DNA and antiproliferative activities of two novel Cu(II) complexes with norcantharidin and benzimidazole derivatives
- Author
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Wen-Ji Song, Fang-Yuan Du, Qiu-Yue Lin, Wen-Jiao Jiang, Qiong Wei, and Qing-Yuan Qi
- Subjects
Models, Molecular ,Benzimidazole ,Carcinoma, Hepatocellular ,Stereochemistry ,Intercalation (chemistry) ,Infrared spectroscopy ,Antineoplastic Agents ,Crystallography, X-Ray ,Analytical Chemistry ,chemistry.chemical_compound ,Coordination Complexes ,Cell Line, Tumor ,Animals ,Humans ,Bovine serum albumin ,Imide ,Instrumentation ,Spectroscopy ,Cell Proliferation ,biology ,Liver Neoplasms ,Serum Albumin, Bovine ,DNA ,Bridged Bicyclo Compounds, Heterocyclic ,Fluorescence ,Atomic and Molecular Physics, and Optics ,Intercalating Agents ,Crystallography ,chemistry ,Agarose gel electrophoresis ,biology.protein ,Hydroxyl radical ,Benzimidazoles ,Cattle ,Copper - Abstract
Two novel complexes [Cu(L)2(Ac)2]·3H2O (1) (L = N-2-methyl benzimidazole demethylcantharate imide, C16H15N3O3, Ac = acetate, C2H3O2) and [Cu(bimz)2(DCA)] (2) (bimz = benzimidazole, C7H6N2; DCA = demethylcantharate, C8H8O5) were synthesized and characterized by elemental analysis, infrared spectra and X-ray diffraction techniques. Cu(II) ion was four-coordinated in complex 1, Cu(II) ion was five-coordinated in complex 2. A large amount of intermolecular hydrogen-bonding and π–π stacking interactions were observed in these complex structures. The DNA-binding properties of these complexes were investigated using electronic absorption spectra, fluorescence spectra, viscosity measurements and agarose gel electrophoresis. The interactions between the complexes and bovine serum albumin (BSA) were investigated by fluorescence spectra. The antiproliferative activities of the complexes against human hepatoma cells (SMMC7721) were tested in vitro. And the results showed that these complexes could bind to DNA in moderate intensity via partial intercalation, and complexes 1 and 2 could cleave plasmid DNA through hydroxyl radical mechanism. Title complexes could effectively quench the fluorescence of BSA through static quenching. Meanwhile, title complexes had stronger antiproliferative effect compared to L and Na2(DCA) within the tested concentration range. And complex 1 possessed more antiproliferative active than complex 2.
- Published
- 2014