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7. Crystallization and preliminary X-ray study of alanine dehydrogenase from Bacillus pseudofirmus OF4.

Author

Wen, Jinjin, Li, Zhenzhen, He, Guangzheng, Xu, Shujing, Zhao, Baohua, Zhu, Xianming, Dong, Hui, and Ju, Jiansong

Subjects
*CRYSTALLIZATION, *ALANINE dehydrogenase, *OXIDOREDUCTASES, *BACILLUS pseudofirmus, *CHROMATOGRAPHIC analysis, *X-ray diffraction
Abstract

Alanine dehydrogenase (OF4Ald) from the alkaliphilic Bacillus pseudofirmus OF4 was expressed and purified with a His6 tag in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop vapour-diffusion method at 289 K using a solution consisting of 0.1 M Tris-HCl pH 8.0, 0.2 M LiSO4, 22%( w/ v) PEG 3350. X-ray diffraction data were collected to 2.8 Å resolution. The crystal belonged to the triclinic space group P1, with unit-cell parameters a = 88.04, b = 105.59, c = 120.53 Å, α = 88.37, β = 78.77, γ = 82.65°. [ABSTRACT FROM AUTHOR]

Published
2013
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