148 results on '"Weiyue Feng"'
Search Results
2. Renal clearance of graphene oxide: glomerular filtration or tubular secretion and selective kidney injury association with its lateral dimension
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Wei Chen, Bing Wang, Shanshan Liang, Meng Wang, Lingna Zheng, Si Xu, Jiali Wang, Hao Fang, Pu Yang, and Weiyue Feng
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Glomerular filtration ,Tubular secretion ,Graphene oxide ,Renal excretion mechanism ,Selective kidney injury ,Lateral dimension ,Biotechnology ,TP248.13-248.65 ,Medical technology ,R855-855.5 - Abstract
Abstract Background Renal excretion is one of the major routes of nanomaterial elimination from the body. Many previous studies have found that graphene oxide nanosheets are excreted in bulk through the kidneys. However, how the lateral size affects GO disposition in the kidneys including glomerular filtration, active tubular secretion and tubular reabsorption is still unknown. Results The thin, two-dimensional graphene oxide nanosheets (GOs) was observed to excrete in urine through the kidneys, but the lateral dimension of GOs affects their renal clearance pathway and renal injury. The s-GOs could be renal excreted via the glomerular filtration, while the l-GOs were predominately excreted via proximal tubular secretion at a much faster renal clearance rate than the s-GOs. For the tubular secretion of l-GOs, the mRNA level of basolateral organic anion transporters Oat1 and Oat2 in the kidney presented dose dependent increase, while no obvious alterations of the efflux transporters such as Mdr1 and Mrp4 mRNA expression levels were observed, suggesting the accumulation of l-GOs. During the GO renal elimination, mostly the high dose of 15 mg/kg s-GO and l-GO treatment showed obvious kidney injuries but at different renal compartment, i.e., the s-GOs induced obvious glomerular changes in podocytes, while the l-GOs induced more obvious tubular injuries including necrosis of renal tubular epithelial cells, loss of brush border, cast formation and tubular dilatation. The specifically tubular injury biomarkers KIM1 and NGAL were shown slight increase with mRNA levels in l-GO administrated mice. Conclusions This study shows that the lateral size of GOs affected their interactions with different renal compartments, renal excretion pathways and potential kidney injuries.
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- 2023
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3. Multifaceted Characterization for the Hepatic Clearance of Graphene Oxide and Size-Related Hepatic Toxicity
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Zongyi Su, Wei Chen, Shanshan Liang, Hao Fang, Minglu Zhang, Meng Wang, Lingna Zheng, Bing Wang, Yi Bi, and Weiyue Feng
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graphene oxide ,hepatic clearance ,multifaceted characterization ,Organic chemistry ,QD241-441 - Abstract
Understanding the final fate of nanomaterials (NMs) in the liver is crucial for their safer application. As a representative two-dimensional (2D) soft nanomaterial, graphene oxide (GO) has shown to have high potential for applications in the biomedical field, including in biosensing, drug delivery, tissue engineering, therapeutics, etc. GO has been shown to accumulate in the liver after entering the body, and thus, understanding the GO–liver interaction will facilitate the development of safer bio-applications. In this study, the hepatic clearance of two types of PEGylated GOs with different lateral sizes (s-GOs: ~70 nm and l-GOs: ~300 nm) was carefully investigated. We found that GO sheets across the hepatic sinusoidal endothelium, which then may be taken up by the hepatocytes via the Disse space. The hepatocytes may degrade GO into dot-like particles, which may be excreted via the hepatobiliary route. In combination with ICP-MS, LA-ICP-MS, and synchrotron radiation FTIR techniques, we found that more s-GO sheets in the liver were prone to be cleared via hepatobiliary excretion than l-GO sheets. A Raman imaging analysis of ID/IG ratios further indicated that both s-GO and l-GO generated more defects in the liver. The liver microsomes may contribute to GO biotransformation into O-containing functional groups, which plays an important role in GO degradation and excretion. In particular, more small-sized GO sheets in the liver were more likely to be cleared via hepatobiliary excretion than l-GO sheets, and a greater clearance of s-GO will mitigate their hepatotoxicity. These results provide a better understanding of the hepatic clearance of soft NMs, which is important in the safer-by-design of GO.
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- 2024
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4. Bi-Functionalized Transferrin@MoS2-PEG Nanosheets for Improving Cellular Uptake in HepG2 Cells
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Si Xu, Shanshan Liang, Bing Wang, Jiali Wang, Meng Wang, Lingna Zheng, Hao Fang, Tingfeng Zhang, Yi Bi, and Weiyue Feng
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bi-functionalization ,transferrin@MoS2-PEG nanosheets ,HepG2 cellular uptake ,Technology ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Microscopy ,QH201-278.5 ,Descriptive and experimental mechanics ,QC120-168.85 - Abstract
Pre-coating with a protein corona on the surface of nanomaterials (NMs) is an important strategy for reducing non-specific serum protein absorption while maintaining targeting specificity. Here, we present lipoic acid-terminated polyethylene glycol and transferrin bi-functionalized MoS2 nanosheets (Tf@MoS2-PEG NSs) as a feasible approach to enhance cellular uptake. Tf@MoS2-PEG NSs can maintain good dispersion stability in cell culture medium and effectively protect MoS2 NSs from oxidation in ambient aqueous conditions. Competitive adsorption experiments indicate that transferrin was more prone to bind MoS2 NSs than bovine serum albumin (BSA). It is noteworthy that single HepG2 cell uptake of Tf@MoS2-PEG presented a heterogeneous distribution pattern, and the cellular uptake amount spanned a broader range (from 0.4 fg to 2.4 fg). Comparatively, the intracellular Mo masses in HepG2 cells treated with BSA@MoS2-PEG and MoS2-PEG showed narrower distribution, indicating homogeneous uptake in the single HepG2 cells. Over 5% of HepG2 cells presented uptake of the Tf@MoS2-PEG over 1.2 fg of Mo, about three-fold that of BSA@MoS2-PEG (0.4 fg of Mo). Overall, this work suggests that Tf coating enhances the cellular uptake of MoS2 NSs and is a promising strategy for improving the intracellular uptake efficiency of cancer cells.
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- 2023
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5. Surface chemistry governs the sub-organ transfer, clearance and toxicity of functional gold nanoparticles in the liver and kidney
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Xue Li, Bing Wang, Shuang Zhou, Wei Chen, Hanqing Chen, Shanshan Liang, Lingna Zheng, Hongyang Yu, Runxuan Chu, Meng Wang, Zhifang Chai, and Weiyue Feng
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Surface chemistry ,Gold nanoparticles ,Sub-organ transfer ,Clearance ,Biotechnology ,TP248.13-248.65 ,Medical technology ,R855-855.5 - Abstract
Abstract Background To effectively applied nanomaterials (NMs) in medicine, one of the top priorities is to address a better understanding of the possible sub-organ transfer, clearance routes, and potential toxicity of the NMs in the liver and kidney. Results Here we explored how the surface chemistry of polyethylene glycol (PEG), chitosan (CS), and polyethylenimine (PEI) capped gold nanoparticles (GNPs) governs their sub-organ biodistribution, transfer, and clearance profiles in the liver and kidney after intravenous injection in mice. The PEG-GNPs maintained dispersion properties in vivo, facilitating passage through the liver sinusoidal endothelium and Disse space, and were captured by hepatocytes and eliminated via the hepatobiliary route. While, the agglomeration/aggregation of CS-GNPs and PEI-GNPs in hepatic Kupffer and endothelial cells led to their long-term accumulation, impeding their elimination. The gene microarray analysis shows that the accumulation of CS-GNPs and PEI-GNPs in the liver induced obvious down-regulation of Cyp4a or Cyp2b related genes, suggesting CS-GNP and PEI-GNP treatment impacted metabolic processes, while the PEI-GNP treatment is related with immune responses. Conclusions This study demonstrates that manipulation of nanoparticle surface chemistry can help NPs selectively access distinct cell types and elimination pathways, which help to clinical potential of non-biodegradable NPs.
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- 2020
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6. Understanding the Role of the Lateral Dimensional Property of Graphene Oxide on Its Interactions with Renal Cells
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Wei Chen, Bing Wang, Shanshan Liang, Meng Wang, Lingna Zheng, Si Xu, Jiali Wang, Hao Fang, Pu Yang, and Weiyue Feng
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graphene oxide ,lateral dimension ,renal cells ,interaction ,cytotoxicity ,Organic chemistry ,QD241-441 - Abstract
Renal excretion is expected to be the major route for the elimination of biomedically applied nanoparticles from the body. Hence, understanding the nanomedicine–kidney interaction is crucially required, but it is still far from being understood. Herein, we explored the lateral dimension- (~70 nm and ~300 nm), dose- (1, 5, and 15 mg/kg in vivo and 0.1~250 μg/mL in vitro), and time-dependent (48 h and 7 d in vivo) deposition and injury of PEGylated graphene oxide sheets (GOs) in the kidney after i.v. injection in mice. We specially investigated the cytotoxic effects on three typical kidney cell types with which GO renal excretion is related: human renal glomerular endothelial cells (HRGECs) and human podocytes, and human proximal tubular epithelial cells (HK-2). By using in vivo fluorescence imaging and in situ Raman imaging and spectroscopic analysis, we revealed that GOs could gradually be eliminated from the kidneys, where the glomeruli and renal tubules are their target deposition sites, but only the high dose of GO injection induced obvious renal histological and ultrastructural changes. We showed that the high-dose GO-induced cytotoxicity included a cell viability decrease and cellular apoptosis increase. GO uptake by renal cells triggered cellular membrane damage (intracellular LDH release) and increased levels of oxidative stress (ROS level elevation and a decrease in the balance of the GSH/GSSG ratio) accompanied by a mitochondrial membrane potential decrease and up-regulation of the expression of pro-inflammatory cytokines TNF-α and IL-18, resulting in cellular apoptosis. GO treatments activated Keap1/Nrf2 signaling; however, the antioxidant function of Nrf2 could be inhibited by apoptotic engagement. GO-induced cytotoxicity was demonstrated to be associated with oxidative stress and an inflammation reaction. Generally, the l-GOs presented more pronounced cytotoxicity and more severe cellular injury than s-GOs did, demonstrating lateral size-dependent toxicity to the renal cells. More importantly, GO-induced cytotoxicity was independent of renal cell type. The results suggest that the dosage of GOs in biomedical applications should be considered and that more attention should be paid to the ability of a high dose of GO to cause renal deposition and potential nephrotoxicity.
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- 2022
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7. Gold Nanoparticles Modified With Polyethyleneimine Disturbed the Activity of Drug-Metabolic Enzymes and Induced Inflammation-Mediated Liver Injury in Mice
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Hanqing Chen, Shuang Zhou, Meilin Zhu, Bing Wang, Wei Chen, Lingna Zheng, Meng Wang, and Weiyue Feng
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gold nanoparticles ,drug-metabolic enzymes ,hepatic transporters ,cytochrome P450 ,liver inflammation ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Gold nanoparticles (GNPs) have been used as a potential bioactive platform for drug delivery due to their unique optical and thermal characteristics. Liver is the main organ in orchestrating physiological homeostasis through metabolization of drugs and detoxification of exogenous substances. Therefore, it is crucial to deeply understand the mechanism of nanoparticle–liver interaction and the potential hepatic effects of GNPs in vivo. In this study, we studied the hepatic impacts of the intravenously injected polyethyleneimine (PEI)-modified GNPs (PEI-GNPs) on the expression of hepatic drug-metabolic enzymes and sterol responsive element binding protein 1c (SREBP-1c)-mediated de novo lipogenesis in mice for 24 h and 1 week. PEI-GNP accumulation in the liver is associated with increased liver inflammation, as evidenced by the gene expression of pro-inflammatory cytokines. Moreover, the GNP-induced hepatotoxicity in mice is partly due to liver inflammation–triggered disruption in the function of drug-metabolic enzymes, including hepatic uptake and efflux transporters, cytochrome P450 (CYP450), and UDP-glucuronosyltransferases (UGTs). The study provides evidence that it is necessary to consider the nanomaterial–liver interaction and manipulate the surface chemistry of GNPs prior to biomedical application of nanoparticles.
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- 2021
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8. Rapamycin-Loaded mPEG-PLGA Nanoparticles Ameliorate Hepatic Steatosis and Liver Injury in Non-alcoholic Fatty Liver Disease
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Ruifang Zhao, Meilin Zhu, Shuang Zhou, Weiyue Feng, and Hanqing Chen
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non-alcoholic fatty liver disease ,mPEG-PLGA ,rapamycin ,hepatic steatosis ,SREBP-1c ,de novo lipogenesis ,Chemistry ,QD1-999 - Abstract
Non-alcoholic fatty liver disease (NAFLD) is characterized by excessive lipid accumulation and liver injury, and is the leading cause of chronic liver disease worldwide. There is an urgent need to develop novel pathophysiology-oriented therapy in human. Rapamycin (RAPA) has been recognized as a promising drug for alleviating hepatic steatosis on NAFLD, but the poorly water-soluble properties and side effects of RAPA limit their clinical use. In this study, we aimed to investigate the in vitro and in vivo therapeutic efficacy of biodegradable mPEG-PLGA polymers loaded with RAPA (NP-RAPA) on NAFLD. NP-RAPA were prepared by a green process using an emulsion/solvent evaporation method, the therapeutic efficacy on NAFLD were investigated on HepG2 cells incubated with oleic acid (OA) and in the livers of mice with NAFLD induced by high-fat diet (HFD). Compared with free RAPA, NP-RAPA significantly reduced lipid accumulation in HepG2 cells, and obviously ameliorated hepatic steatosis and liver injury in mice though enhancing the therapeutic efficacy of RAPA through reducing SREBP-1c-dependent de novo lipogenesis (DNL) and promoting PPARα-mediated fatty acid oxidation. This study suggests that mPEG-PLGA can be used as the potential therapeutic strategy and novel drug delivery for improving the efficacy of rapamycin for treatment of NAFLD.
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- 2020
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9. Manufactured nanoparticle: A prediction model for understanding PM2.5 toxicity to human
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Weiyue Feng and Yuliang Zhao
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Renewable energy sources ,TJ807-830 ,Ecology ,QH540-549.5 - Published
- 2017
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10. Laser ablation-single particle-inductively coupled plasma mass spectrometry as a sensitive tool for bioimaging of silver nanoparticles in vivo degradation
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Meng Wang, Lingna Zheng, Bing Wang, Pu Yang, Hao Fang, Shanshan Liang, Wei Chen, and Weiyue Feng
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General Chemistry - Published
- 2022
11. Professor Zhifang Chai: Scientific contributions and achievements
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Yuliang Zhao, Chunying Chen, Weiyue Feng, Zhiyong Zhang, Diandou Xu, Weiqun Shi, Shuao Wang, and Yu-Feng Li
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General Chemistry - Published
- 2022
12. Toxicology of Nanomaterials
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Yuliang Zhao, Zhiyong Zhang, Weiyue Feng, Yuliang Zhao, Zhiyong Zhang, Weiyue Feng and Yuliang Zhao, Zhiyong Zhang, Weiyue Feng, Yuliang Zhao, Zhiyong Zhang, Weiyue Feng
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- 2016
13. Clusterbody Enables Flow Sorting-Assisted Single-Cell Mass Spectrometry Analysis for Identifying Reversal Agent of Chemoresistance
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Han Li, Jiaojiao Li, Meng Wang, Weiyue Feng, Fuping Gao, Ying Han, Yijie Shi, Zhongying Du, Qing Yuan, Peng Cao, Xiayan Wang, Xueyun Gao, Kai Cao, and Liang Gao
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Analytical Chemistry - Abstract
Identifying effective reversal agents overcoming multidrug resistance with causal mechanisms from an efflux pump protein is of vital importance for enhanced tumor chemotherapy in clinic. To achieve this end, we construct a metal cluster-based probe, named clusterbody, to develop flow sorting-assisted single-cell mass spectrometry analysis. This clusterbody synthesized by biomimetic mineralization possesses an antibody-like property to selectively recognize an efflux pump protein. The intrinsic red fluorescence emission of the clusterbody facilitates fluorescence-activated high-throughput cell sorting of subpopulations with different multidrug resistance levels. Furthermore, based on the accurate formula of the clusterbody, the corresponding protein abundance at the single-cell level is determined through detecting gold content via precise signal amplification by laser ablation inductively coupled plasma mass spectrometry. Therefore, the effect of reversal agent treatment overcoming multidrug resistance is evaluated in a quantitative manner. This work opens a new avenue to identify reversal agents, shedding light on developing combined or synergetic tumor therapy.
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- 2022
14. 3D-imaging and quantitative assessment for size-related penetration of HfO2 nanoparticles in breast cancer tumor by synchrotron radiation microcomputed tomography
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Jiali Wang, Tingfeng Zhang, You Liao, Wei Chen, Shanshan Liang, Si Xu, Hao Fang, Meng Wang, Lingna Zheng, Zhanjun Gu, Zhiyong Zhang, Bing Wang, Yi Bi, and Weiyue Feng
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Environmental Chemistry ,Biochemistry ,Spectroscopy ,Analytical Chemistry - Published
- 2023
15. Iron oxide nanoparticles aggravate hepatic steatosis and liver injury in nonalcoholic fatty liver disease through BMP-SMAD-mediated hepatic iron overload
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Wei Chen, Lingna Zheng, Bing Wang, Hanqing Chen, Meilin Zhu, Xue Li, Runxuan Chu, Meng Wang, Weiyue Feng, Zhifang Chai, and Shuang Zhou
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medicine.medical_specialty ,Iron Overload ,Biomedical Engineering ,02 engineering and technology ,SMAD ,010501 environmental sciences ,Toxicology ,01 natural sciences ,Mice ,chemistry.chemical_compound ,Non-alcoholic Fatty Liver Disease ,Internal medicine ,Nonalcoholic fatty liver disease ,medicine ,Animals ,Hepatic iron ,0105 earth and related environmental sciences ,Liver injury ,business.industry ,nutritional and metabolic diseases ,021001 nanoscience & nanotechnology ,medicine.disease ,digestive system diseases ,Mice, Inbred C57BL ,Endocrinology ,Liver ,chemistry ,Lipogenesis ,Magnetic Iron Oxide Nanoparticles ,Metabolic syndrome ,Steatosis ,0210 nano-technology ,business ,Iron oxide nanoparticles - Abstract
Nonalcoholic fatty liver disease (NAFLD) is the leading hepatic manifestation of metabolic syndrome worldwide, and is clinically accompanied by iron overload. As the increasing application of iron oxide nanoparticles (IONPs) on the imaging and diagnosis in NAFLD, the potential hepatic effect and mechanism of IONPs on NAFLD should be well studied. Here, we demonstrate that carboxyl-modified (COOH-IONPs) and amino-coated IONPs (NH2-IONPs) exhibit no significant hepatic toxicity in normal mice at the clinical injection dose, but aggravate SREBP-1c-mediated de novo lipogenesis (DNL) in the livers of mice with NAFLD induced by high-fat diet (HFD) and in HepG2 cells incubated with oleic acid (OA), especially in those treated by the positive NH2-IONPs. In the present study, mice receiving IONPs for 7 day show mild iron overload in the liver and exhibit enhanced hepatic inflammation in NAFLD. The BMP-SMAD pathway is initiated by hepatic iron overload and is aggravated in NAFLD. In conclusion, BMP-SMAD-mediated hepatic iron overload aggravated lipid accumulation in the liver and hepatic inflammatory responses, implying that effective measures in addition to hepatic iron overload are needed for individuals at the risk of IONPs in NAFLD.
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- 2021
16. Interaction of Humic Acid with Graphene Oxide: Relation to Antibacterial Activities Against Escherichia coli
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Wei Du, Bing Wang, Meng Wang, Lingna Zheng, Huiyan Chen, Shuang Zhou, Xue Li, Wei Chen, Runxuan Chu, Hongyang Yu, Weiyue Feng, and Hanqing Chen
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chemistry.chemical_classification ,Aqueous solution ,Materials science ,Graphene ,Exothermic process ,Biomedical Engineering ,Oxide ,Bioengineering ,Isothermal titration calorimetry ,General Chemistry ,Condensed Matter Physics ,medicine.disease_cause ,law.invention ,chemistry.chemical_compound ,chemistry ,law ,medicine ,Humic acid ,General Materials Science ,Antibacterial activity ,Escherichia coli ,Nuclear chemistry - Abstract
Graphene oxide (GO) sheets attracted great attention as effectively antibacterial agents in water treatment and environmental remediation applications. In the study, the interaction of humic acid (HA) as the model of natural organic matter (NOM) with GO and their antibacterial activities against Escherichia coli (E. coli) was investigated. The interaction between GO and HA molecules was analyzed by isothermal titration calorimetry (ITC) and fluorescence spectroscopy analysis. The study demonstrated that GO reaction with HA was a spontaneously exothermic process, which enabled formation of stable and well dispersed GO-HA complex in aqueous solution. Both GO and GO-HA could significantly inhibit the growth of E. coli and present dose-dependent bactericidal property. GO and GO-HA showed more obvious antibacterial activity in saline solution than in LB broth. We suggest the surface wrinkles of GO and GO-HA could contribute to the firm wrapping of E. coli, which is the principle factor for the antibacterial activity of GO and GO-HA. Especially, GO-HA exhibit less surface wrinkles in comparison with GO, corresponding to its reduced antibacterial activity in saline solution.
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- 2021
17. Single Particle Inductively Coupled Plasma Time-of-Flight Mass Spectrometry—A Powerful Tool for the Analysis of Nanoparticles in the Environment
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Ziwei Meng, Lingna Zheng, Hao Fang, Pu Yang, Bing Wang, Liang Li, Meng Wang, and Weiyue Feng
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Process Chemistry and Technology ,Chemical Engineering (miscellaneous) ,Bioengineering - Abstract
Single-particle inductively coupled plasma-mass spectrometry (SP-ICP-MS) has emerged as an important tool for the characterization of inorganic nanoparticles (NPs) in the environment. Although most SP-ICP-MS applications rely on the quadrupole ICP-MS (ICP-QMS), it is limited by the slow scanning speed of the quadrupole. Recent advancements in instrumentation have led to the development of inductively coupled plasma time-of-flight mass spectrometry (ICP-TOF-MS) which offers a viable solution. In this review, we discuss the recent advances in instrumentation and methodology of ICP-TOF-MS, followed by a detailed discussion of the applications of SP-ICP-TOFMS in analyzing NPs in the environment. SP-ICP-TOFMS has the potential to identify and quantify both anthropogenic and natural NPs in the environment, providing valuable insights into their occurrence, fate, behavior, and potential environmental risks.
- Published
- 2023
18. Single-Cell Isotope Dilution Analysis with LA–ICP–MS: A New Approach for Quantification of Nanoparticles in Single Cells
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Hanqing Chen, Liu-Xing Feng, Lingna Zheng, Haifang Wang, Bing Wang, Junwen Shi, Weiyue Feng, and Meng Wang
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Detection limit ,education.field_of_study ,Silver ,Chromatography ,Isotope ,Chemistry ,Macrophages ,Cell ,Population ,Microfluidics ,Metal Nanoparticles ,Nanoparticle ,Biological Transport ,Isotope dilution ,Mass spectrometry ,Mass Spectrometry ,Analytical Chemistry ,Mice ,RAW 264.7 Cells ,medicine.anatomical_structure ,Isotopes ,medicine ,Animals ,Single-Cell Analysis ,education - Abstract
Laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) is an emerging method for the analysis of metal nanoparticles (NPs) in single cells. However, two main obstacles, low analytical throughput and lack of commercial reference materials, need to be overcome. In this work, we demonstrated the principles of a new approach termed "single-cell isotope dilution analysis" (SCIDA) to remove the two obstacles. For a proof of concept, macrophage cells were chosen as a model to study the uptake of silver NPs (AgNPs) at a single-cell level. Single cells exposed to AgNPs were placed in an array by a microfluidic technique; each cell in the array was precisely dispensed with a known picoliter droplet of an enriched isotope solution with a commercial inkjet printer; accurate quantification of AgNPs in single cells was done by using isotope dilution LA-ICP-MS. The average Ag mass of 1100 single cells, 396 ± 219 fg Ag per cell, was in good accord with the average of the population of cells determined by solution ICP-MS analysis. The detection limit was 0.2 fg Ag per cell. The SCIDA approach is expected to be widely applied for the study of cell-NP interactions and biological effects of NPs at the single-cell level.
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- 2020
19. Immunological Responses Induced by Blood Protein Coronas on Two-Dimensional MoS2 Nanosheets
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Mengyu Guo, Bing Wang, Ye Tao, Jiaming Liu, Junguang Wu, Mingjing Cao, Liming Wang, Runxuan Chu, Chendong Ji, Chunying Chen, Didar Baimanov, Rong Cai, Weiyue Feng, Yuliang Zhao, Jing Wang, and Xia Yuan
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chemistry.chemical_classification ,biology ,Chemistry ,General Engineering ,General Physics and Astronomy ,Biological membrane ,Protein Corona ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Human serum albumin ,01 natural sciences ,Blood proteins ,Immunoglobulin G ,0104 chemical sciences ,Transferrin ,medicine ,Biophysics ,biology.protein ,General Materials Science ,0210 nano-technology ,Receptor ,Protein adsorption ,medicine.drug - Abstract
Two-dimensional (2D) nanosheets (NSs) have a large surface area, high surface free energy, and ultrathin structure, which enable them to more easily penetrate biological membranes and promote adsorption of drugs and proteins. NSs are capable of adsorbing a large amount of blood proteins to form NSs-protein corona complexes; however, their inflammatory effects are still unknown. Therefore, we investigated the pro-inflammatory effect of 2D model nanosheet structures, molybdenum disulfide (MoS2), and the MoS2 NSs-protein complexes with four abundant proteins in human blood, i.e., human serum albumin (HSA), transferrin (Tf), fibrinogen (Fg), and immunoglobulin G (IgG). The interactions between the NSs and the proteins were analyzed by quantifying protein adsorption, determining binding affinity, and correlating structural changes in the protein corona with the uptake of NSs by macrophages and the subsequent inflammatory response. Although all of the NSs-protein complexes induced inflammation, IgG-coated and Fg-coated NSs triggered much stronger inflammatory effects by producing and releasing more cytokines. Among the four proteins, IgG possessed the highest proportion of β-sheets and led to fewer secondary structure changes on the MoS2 nanosheets. This can facilitate uptake and produce a stronger pro-inflammatory response in macrophages due to the recognition of an NSs-IgG complex by Fc gamma receptors and the subsequent activation of the NF-κB pathways. Our results demonstrate that the blood protein components contribute to the inflammatory effects of nanosheets and provide important insights for the nanosafety evaluation and the rational design of nanomedicines in the future.
- Published
- 2020
20. Adsorption and oxidation of SO2 on the surface of TiO2 nanoparticles: the role of terminal hydroxyl and oxygen vacancy–Ti3+ states
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Hanqing Chen, Shanshan Liang, Runxuan Chu, Weiyue Feng, Dan Zhang, Bing Wang, Wei Chen, Xue Li, Xingzhong Cao, Shuang Zhou, and Meng Wang
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Chemistry ,Radical ,General Physics and Astronomy ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Photochemistry ,01 natural sciences ,Redox ,XANES ,Dissociation (chemistry) ,0104 chemical sciences ,Catalysis ,Adsorption ,X-ray photoelectron spectroscopy ,Molecule ,Physical and Theoretical Chemistry ,0210 nano-technology - Abstract
Herein, the absorption and oxidation reactions of SO2 on TiO2 nanoparticles (TiO2 NPs) at 296 K under various environmental conditions (humidity, UV irradiation, and ozone copresence) were investigated by using a flow chamber reaction system, synchrotron X-ray absorption near-edge structure (XANES) and high resolution synchrotron X-ray photoelectron spectroscopy (XPS) measurements. The results showed that oxidation of SO2 to sulfate via TiO2 NP catalysis happened at a very rapid rate. The appropriate relative humidity, UV irradiation and co-presence of ozone all markedly promoted SO2 oxidation on TiO2 NPs. High resolution XPS unraveled that the terminal hydroxyl (OHt) and oxygen vacancy (VO)–Ti3+ states on TiO2 NPs were the active sites for SO2 adsorption and oxidation. The data of XPS measurements suggest that SO2 was adsorbed on a OHt next to a Ti3+ VO and reacted to form HSO3−. HSO3− can then transform into SO32−via transfer of a proton. The resulting adsorbed SO32− could bind to a surface bridging O (Ob) atom and transform into SO42−. A H2O molecule could dissociate on VO–Ti3+ into two bridging hydroxyl (OHb) groups, subsequently forming new Ob, which provides an active O site for the adsorbed HSO3−/SO32− and oxidizes them into HSO4−/SO42− on the surface of the TiO2 NPs. The copresence of O3 could promote H2O dissociation into OHb, promoting the formation of Ob. The copresence of O3 may also promote the dissociation of adsorbed H2O into TiO2–O2− and hydroxyl radicals (˙OH) on VOs, facilitating the oxidation of adsorbed HSO3−/SO32−. Under UV irradiation, new VOs were created via oxidation of lattice O by photo-generated holes, resulting in increased Ob and subsequently enhanced oxidation of adsorbed HSO3−/SO32− on TiO2 NPs.
- Published
- 2020
21. Chemical Analysis and Imaging of Fingerprints by Air-flow Assisted Desorption Electrospray Ionization Mass Spectrometry
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Lingna Zheng, Hanqing Chen, Rong-Liang Ma, Meng Wang, Junwen Shi, Weiyue Feng, Bing Wang, and Haifang Wang
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Chromatography ,Chemistry ,010401 analytical chemistry ,Airflow ,Desorption electrospray ionization mass spectrometry ,High resolution ,02 engineering and technology ,021001 nanoscience & nanotechnology ,Mass spectrometry ,01 natural sciences ,0104 chemical sciences ,Analytical Chemistry ,Fingerprint ,0210 nano-technology - Abstract
Fingerprint analysis is of great significance in forensic sciences. Compared with existing fingerprint analytical methods, mass spectrometry-based methods can not only identify chemical components in fingerprints, but also obtain fingerprint imaging. In this study four kinds of fingerprints, including sweat, inkpad, sunscreen, and liquid foundation, were analyzed by air-flow assisted desorption electrospray ionization mass spectrometry imaging (AFADESI-MSI). AFADESI was employed with air flow of 45 L min−1, and 5 μL min−1 acetonitrile was used as spray solvent at spray voltage of 7000 V. Positive ion full scan mode (100−1000 Da) was chosen. The results showed that AFADESI-MSI method could not only obtain chemical information of various endogenous and exogenous substances in fingerprints, but also obtain high resolution images of fingerprints. In addition, overlapped fingerprints were distinguished according to the typical chemical information in fingerprints. As a new fingerprint analysis method, AFADESI-MSI would be widely used in forensic scientific research and practical applications.
- Published
- 2019
22. Determination of Trace Mercury in Water by On-line Solid Phase Extraction and Ultraviolet Vapor Generation–ICP-MS
- Author
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Dan-Dan Shao, Weiyue Feng, Lingna Zheng, Zhong-Yuan Wang, Bing Wang, Meng Wang, and Hong-Kang Zhang
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Chemistry ,Analytical chemistry ,medicine ,chemistry.chemical_element ,Solid phase extraction ,medicine.disease_cause ,Inductively coupled plasma mass spectrometry ,Spectroscopy ,Ultraviolet ,Mercury (element) - Published
- 2019
23. Elemental analysis and imaging of sunscreen fingermarks by X-ray fluorescence
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Meng Wang, Yuliang Zhao, Rong Liang Ma, Qian Li, Dong Liang Chen, Yuan Bo Sang, Qi Qi Yan, Ling Na Zheng, Weiyue Feng, Hailong Wang, and Bing Wang
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Titanium ,Elemental imaging ,Acid digestion ,Materials science ,010401 analytical chemistry ,Spectrometry, X-Ray Emission ,X-ray fluorescence ,Nanotechnology ,02 engineering and technology ,021001 nanoscience & nanotechnology ,Proof of Concept Study ,01 natural sciences ,Biochemistry ,0104 chemical sciences ,Analytical Chemistry ,Zinc ,Elemental analysis ,0210 nano-technology ,Sunscreening Agents - Abstract
Chemical composition in fingermarks could provide useful information for forensic studies and applications. Here, we evaluate the feasibility of analysis and imaging of fingermarks via elements by synchrotron radiation X-ray fluorescence (SRXRF) and commercial X-ray fluorescence (XRF). As a proof of concept, we chose four brands of sunscreens to make fingermarks on different substrates, including plastic film, glass, paper, and silicon wafer. We obtained an evident image of fingermarks via zinc and titanium by XRF methods. In addition, the ratios of element concentrations in sunscreen fingermarks were obtained, which were in accordance with the results obtained by acid digestion and ICP-OES analysis. In comparison, commercial XRF offers the most advantages in terms of non-destructive detection, easy accessibility, fast element images, and broad applicability. The possibility to acquire fingermark images simultaneously with element information opens up new avenues for forensic science. Graphical abstract.
- Published
- 2019
24. Determination of silver nanoparticles in single cells by microwell trapping and laser ablation ICP-MS determination
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Rui-Ping Luo, Bing Wang, Yuan-Bo Sang, Lingna Zheng, Weiyue Feng, Fu-Ting Yi, and Meng Wang
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Detection limit ,Materials science ,Chromatography ,Laser ablation ,Polydimethylsiloxane ,010401 analytical chemistry ,010501 environmental sciences ,Mass spectrometry ,Laser ,01 natural sciences ,Silver nanoparticle ,0104 chemical sciences ,Analytical Chemistry ,law.invention ,chemistry.chemical_compound ,Single-cell analysis ,chemistry ,law ,Inductively coupled plasma mass spectrometry ,Spectroscopy ,0105 earth and related environmental sciences - Abstract
Silver nanoparticles (AgNPs) are currently one of the most manufactured nanomaterials, and they have been applied in various consumer products due to their distinctive antimicrobial properties. Because of cell heterogeneity, the analysis of AgNPs in single cells after exposure is fundamental for biomedical applications or toxicological assays. Here, we developed a high-throughput method for single cell analysis by laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). Single cells were efficiently trapped by a polydimethylsiloxane (PDMS) microwell array. Under optimal conditions, 60% microwells contained one single cell and 4% microwells contained two cells or more. The cells in the array were regularly distributed; thus, they could be easily targeted and ablated in a grid pattern and quantitatively analyzed by LA-ICP-MS with a high throughput. The silver mass in single 16HBE cells exposed to AgNPs showed log-normal distribution ranging from 0.80 to 383 fg Ag per cell. The average result from single cells was in good agreement with the result from the digestion of 3.0 × 106 cells. The limit of detection (LOD) and limit of quantification (LOQ) were calculated to be 0.2 fg Ag and 0.7 fg Ag, respectively. Because of its great potential in single cell analysis, LA-ICP-MS can be widely applied for single cell analysis and can offer great benefits for the study of the biological effects of metal NPs at the single-cell level.
- Published
- 2019
25. Metabolism of Nanomaterials in vivo: Blood Circulation and Organ Clearance *
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Bing Wang, Xiao He, Zhiyong Zhang, Yuliang Zhao, and Weiyue Feng
- Published
- 2021
26. Multiscale Synchrotron-Based Imaging Analysis for the Transfer of PEGylated Gold Nanoparticles
- Author
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Xue Li, Hanqing Chen, Shanshan Liang, Wei Chen, Bing Wang, Meilin Zhu, Runxuan Chu, Meng Wang, Hongyang Yu, Weiyue Feng, Shuang Zhou, and Lingna Zheng
- Subjects
Biodistribution ,Renal cortex ,0206 medical engineering ,Biomedical Engineering ,Metal Nanoparticles ,02 engineering and technology ,Polyethylene Glycols ,Biomaterials ,Mice ,medicine ,Renal medulla ,Animals ,Tissue Distribution ,Kidney ,Chemistry ,021001 nanoscience & nanotechnology ,020601 biomedical engineering ,medicine.anatomical_structure ,Colloidal gold ,Renal pyramids ,PEGylation ,Nanomedicine ,Gold ,0210 nano-technology ,Synchrotrons ,Biomedical engineering - Abstract
High spatial resolution imaging analysis is urgently needed to explore the biodistribution, transfer and clearance profiles, and biological impact of nanoparticles in the body, which will be helpful to clarify the efficacy of nanomedicine in clinical applications. Herein, by combination with multiscale synchrotron-based imaging techniques, including X-ray fluorescence (XRF) spectrometry, Fourier transform infrared (FTIR) spectroscopy, and micro X-ray phase contrast computed tomography (micro-XPCT), we visually displayed the transfer patterns and site-specific distribution of PEGylated gold nanoparticles (PEG-GNPs) in the suborgans of the liver, spleen, and kidney after an intravenous injection in mice. A combination of XRF and FTIR imaging analysis showed that the PEG bands presented similar distribution patterns with Au in the intraorgans, suggesting the stability of PEGylation on GNPs. We show that the PEG-GNPs presented heterogeneous distribution in the hepatic lobules with a large amount around the portal vein zone and then a gradient decrease in the sinusoidal region and the CV zone; in the spleen, it gradually accumulated in the splenic red pulp over time; and in the kidney, it quickly transported via the bloodstream to the renal pyramids and renal pelvis, and parts of PEG-GNPs finally accumulated in the renal medulla and renal cortex. Multidimensional micro-XPCT images further show that the PEG-GNP transfer in the liver induced hepatic blood vessel dilatation while they transferred in the liver, providing evidence of GNP transport across the blood vessel endothelial barrier.
- Published
- 2021
27. Quantitative Analysis of Gold Nanoparticles in Single Cells with Time-resolved ICP-MS
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Jian-Hua Wang, Junwen Shi, Meng Wang, Ming-Li Chen, Weiyue Feng, Xue Li, Xing Wei, Lingna Zheng, and Jinhui Liu
- Subjects
Chromatography ,Chemistry ,Colloidal gold ,Inductively coupled plasma mass spectrometry ,Quantitative analysis (chemistry) ,Spectroscopy - Published
- 2021
28. Interaction of Humic Acid with Graphene Oxide: Relation to Antibacterial Activities Against
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Hongyang, Yu, Runxuan, Chu, Xue, Li, Bing, Wang, Wei, Chen, Shuang, Zhou, Huiyan, Chen, Hanqing, Chen, Meng, Wang, Lingna, Zheng, Wei, Du, and Weiyue, Feng
- Subjects
Escherichia coli ,Graphite ,Oxides ,Humic Substances ,Anti-Bacterial Agents - Abstract
Graphene oxide (GO) sheets attracted great attention as effectively antibacterial agents in water treatment and environmental remediation applications. In the study, the interaction of humic acid (HA) as the model of natural organic matter (NOM) with GO and their antibacterial activities against
- Published
- 2021
29. Adsorption and oxidation of SO
- Author
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Bing, Wang, Xue, Li, Shanshan, Liang, Runxuan, Chu, Dan, Zhang, Hanqing, Chen, Meng, Wang, Shuang, Zhou, Wei, Chen, Xingzhong, Cao, and Weiyue, Feng
- Abstract
Herein, the absorption and oxidation reactions of SO2 on TiO2 nanoparticles (TiO2 NPs) at 296 K under various environmental conditions (humidity, UV irradiation, and ozone copresence) were investigated by using a flow chamber reaction system, synchrotron X-ray absorption near-edge structure (XANES) and high resolution synchrotron X-ray photoelectron spectroscopy (XPS) measurements. The results showed that oxidation of SO2 to sulfate via TiO2 NP catalysis happened at a very rapid rate. The appropriate relative humidity, UV irradiation and co-presence of ozone all markedly promoted SO2 oxidation on TiO2 NPs. High resolution XPS unraveled that the terminal hydroxyl (OHt) and oxygen vacancy (VO)-Ti3+ states on TiO2 NPs were the active sites for SO2 adsorption and oxidation. The data of XPS measurements suggest that SO2 was adsorbed on a OHt next to a Ti3+ VO and reacted to form HSO3-. HSO3- can then transform into SO32-via transfer of a proton. The resulting adsorbed SO32- could bind to a surface bridging O (Ob) atom and transform into SO42-. A H2O molecule could dissociate on VO-Ti3+ into two bridging hydroxyl (OHb) groups, subsequently forming new Ob, which provides an active O site for the adsorbed HSO3-/SO32- and oxidizes them into HSO4-/SO42- on the surface of the TiO2 NPs. The copresence of O3 could promote H2O dissociation into OHb, promoting the formation of Ob. The copresence of O3 may also promote the dissociation of adsorbed H2O into TiO2-O2- and hydroxyl radicals (˙OH) on VOs, facilitating the oxidation of adsorbed HSO3-/SO32-. Under UV irradiation, new VOs were created via oxidation of lattice O by photo-generated holes, resulting in increased Ob and subsequently enhanced oxidation of adsorbed HSO3-/SO32- on TiO2 NPs.
- Published
- 2020
30. Immunological Responses Induced by Blood Protein Coronas on Two-Dimensional MoS
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Didar, Baimanov, Junguang, Wu, Runxuan, Chu, Rong, Cai, Bing, Wang, Mingjing, Cao, Ye, Tao, Jiaming, Liu, Mengyu, Guo, Jing, Wang, Xia, Yuan, Chendong, Ji, Yuliang, Zhao, Weiyue, Feng, Liming, Wang, and Chunying, Chen
- Subjects
Molybdenum ,Humans ,Protein Corona ,Serum Albumin, Human ,Adsorption ,Nanostructures - Abstract
Two-dimensional (2D) nanosheets (NSs) have a large surface area, high surface free energy, and ultrathin structure, which enable them to more easily penetrate biological membranes and promote adsorption of drugs and proteins. NSs are capable of adsorbing a large amount of blood proteins to form NSs-protein corona complexes; however, their inflammatory effects are still unknown. Therefore, we investigated the pro-inflammatory effect of 2D model nanosheet structures, molybdenum disulfide (MoS
- Published
- 2020
31. Surface chemistry governs the sub-organ transfer, clearance and toxicity of functional gold nanoparticles in the liver and kidney
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Hanqing Chen, Xue Li, Lingna Zheng, Wei Chen, Shuang Zhou, Runxuan Chu, Bing Wang, Meng Wang, Weiyue Feng, Shanshan Liang, Hongyang Yu, and Zhifang Chai
- Subjects
Male ,Gene Expression ,Metal Nanoparticles ,Pharmaceutical Science ,Medicine (miscellaneous) ,02 engineering and technology ,Kidney ,01 natural sciences ,Applied Microbiology and Biotechnology ,Polyethylene Glycols ,Mice ,chemistry.chemical_compound ,Cytosol ,Polyethyleneimine ,Tissue Distribution ,Mice, Inbred ICR ,021001 nanoscience & nanotechnology ,lcsh:R855-855.5 ,Liver ,Colloidal gold ,Toxicity ,Clearance ,Molecular Medicine ,0210 nano-technology ,Biodistribution ,lcsh:Medical technology ,lcsh:Biotechnology ,Biomedical Engineering ,Bioengineering ,macromolecular substances ,Polyethylene glycol ,010402 general chemistry ,In vivo ,lcsh:TP248.13-248.65 ,PEG ratio ,Gold nanoparticles ,Animals ,Particle Size ,Rats, Wistar ,Sub-organ transfer ,Chitosan ,Polyethylenimine ,Research ,technology, industry, and agriculture ,Surface chemistry ,Molecular medicine ,Rats ,0104 chemical sciences ,Disease Models, Animal ,Kinetics ,chemistry ,Biophysics ,Gold ,Transcriptome - Abstract
Background To effectively applied nanomaterials (NMs) in medicine, one of the top priorities is to address a better understanding of the possible sub-organ transfer, clearance routes, and potential toxicity of the NMs in the liver and kidney. Results Here we explored how the surface chemistry of polyethylene glycol (PEG), chitosan (CS), and polyethylenimine (PEI) capped gold nanoparticles (GNPs) governs their sub-organ biodistribution, transfer, and clearance profiles in the liver and kidney after intravenous injection in mice. The PEG-GNPs maintained dispersion properties in vivo, facilitating passage through the liver sinusoidal endothelium and Disse space, and were captured by hepatocytes and eliminated via the hepatobiliary route. While, the agglomeration/aggregation of CS-GNPs and PEI-GNPs in hepatic Kupffer and endothelial cells led to their long-term accumulation, impeding their elimination. The gene microarray analysis shows that the accumulation of CS-GNPs and PEI-GNPs in the liver induced obvious down-regulation of Cyp4a or Cyp2b related genes, suggesting CS-GNP and PEI-GNP treatment impacted metabolic processes, while the PEI-GNP treatment is related with immune responses. Conclusions This study demonstrates that manipulation of nanoparticle surface chemistry can help NPs selectively access distinct cell types and elimination pathways, which help to clinical potential of non-biodegradable NPs.
- Published
- 2020
32. Additional file 1 of Surface chemistry governs the sub-organ transfer, clearance and toxicity of functional gold nanoparticles in the liver and kidney
- Author
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Li, Xue, Wang, Bing, Zhou, Shuang, Chen, Wei, Hanqing Chen, Shanshan Liang, Lingna Zheng, Hongyang Yu, Runxuan Chu, Wang, Meng, Zhifang Chai, and Weiyue Feng
- Subjects
Data_FILES - Abstract
Additional file 1. Additional figures and tables.
- Published
- 2020
- Full Text
- View/download PDF
33. Quantitative imaging of trace elements in brain sections of Alzheimer’s disease mice with laser ablation inductively coupled plasma-mass spectrometry
- Author
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Meng Wang, Jian-Hua Wang, Bing Wang, Lingna Zheng, Ming-Li Chen, Weiyue Feng, Xing Wei, Jinhui Liu, and Hanqing Chen
- Subjects
Detection limit ,Materials science ,Laser ablation ,Quantitative imaging ,Resolution (mass spectrometry) ,medicine.medical_treatment ,Laser ablation inductively coupled plasma mass spectrometry ,Analytical chemistry ,Laser ,Ablation ,Analytical Chemistry ,law.invention ,Triple quadrupole mass spectrometer ,law ,medicine ,Spectroscopy - Abstract
Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has become a powerful technique for imaging of trace elements in biological samples. However, accurately quantitative imaging is still a complicated task due to lack of matrix-matched standard materials. On the other hand, traditional LA-ICP-MS analysis usually suffers from slow analytical speed and low imaging resolution mainly due to a long washout time of an ablation cell and a low repetition rate of a laser. In this work, a series of homemade matrix-matched standards were produced by homogeneous spiking of gelatin with a known amount of trace elements (Fe, Cu, and Zn). Using these standards, quantitative imaging of Fe, Cu, and Zn in the brains of Alzheimer's disease (AD) and control mice were achieved using a laser ablation system with a low-dispersion cell and a high repetition rate laser coupled to a triple quadrupole ICP-MS. The limits of detection of Fe, Cu, and Zn are 5.4 μg g−1, 0.081 μg g−1, and 0.54 μg g−1, respectively. The concentrations of Fe, Cu, and Zn in mouse brains are 10 ∼ 70 μg g−1, 5 ∼ 30 μg g−1, and 25 ∼ 150 μg g−1, respectively. The quantitative imaging shows different distribution and contents of these metals between AD and control mouse brains. The developed LA-ICP-MS approach is expected to provide a new insight into biological effects of metals and aetiology of metal-related diseases.
- Published
- 2022
34. Polyvinylpyrrolidone functionalization induces deformable structure of graphene oxide nanosheets for lung-targeting delivery
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Lingna Zheng, Shuang Zhou, Meng Wang, Zhifang Chai, Meilin Zhu, Weiyue Feng, Bing Wang, Lina Zhao, Xue Li, Shanshan Liang, Hongyang Yu, Wei Chen, and Hanqing Chen
- Subjects
Confocal ,Biomedical Engineering ,Pharmaceutical Science ,Bioengineering ,macromolecular substances ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Nanomaterials ,law.invention ,Accessible surface area ,law ,Microscopy ,medicine ,General Materials Science ,Polyvinylpyrrolidone ,Chemistry ,Graphene ,technology, industry, and agriculture ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Drug delivery ,Biophysics ,Surface modification ,0210 nano-technology ,Biotechnology ,medicine.drug - Abstract
Nanomaterials (NMs) as lung-targeted drug delivery vehicles have attracted great attentions. Recent studies indicated that elastic or deformable property of NMs could play a vital role in biomedical applications. Herein, polyvinylpyrrolidone (PVP) functionalized graphene oxide (GO-PVP) via a facile one-pot method and PEGylated GO (GO-PEG) nanosheets (NSs) were synthesized at room temperature. By using rare earth elemental labeling method, we quantitatively revealed that GO-PVP NSs were targeting retention in the lung that after 4 h intravenous injection, the lung/liver and lung/spleen ratios in GO-PVP treated mice were about 230-fold and 30-fold higher of those in GO-PEG treated mice, respectively, especially a considerable amount was retained in the lower respiratory tract including pulmonary interstitium and alveolar region as fibril-like shapes. The florescence imaging and in situ confocal Raman microscopy combining with laser-ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) elemental imaging analysis further confirmed that a more amount of GO-PVP than GO-PEG retention in the pulmonary interstitium. Utilizing atomic force microscopy PeakForce quantitative nanomechanical mapping technique, the nanomechanical property investigation shows that GO-PVP NSs are softer, more adhesive and deformable than GO-PEG NSs. Further, the molecular dynamics simulations illustrate that GO-PVP has deformable structure due to the hydrogen-bond interaction at basal plane between PVP and GO that cause GO-PVP surface roughness and to possess low solvent accessible surface area (SASA), comparatively, the GO-PEG owns relatively firm structure and higher SASA. This study indicates that the well dispersion property and deformable structure of GO-PVP in the lung contribute to its filtration of the endothelial/epithelial barrier and retention in the interstitium and alveolar region. Importantly, the study inspires us to design elastic or deformable NMs in response to the lung disease especially those have occurred in the lower respiratory tract.
- Published
- 2021
35. The effects of orally administered Ag, TiO 2 and SiO 2 nanoparticles on gut microbiota composition and colitis induction in mice
- Author
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Xiaoyan Zhou, Bing Wang, Hanqing Chen, Hailong Wang, Hong Ouyang, Lingna Zheng, Yuliang Zhao, Chengxu Cai, Weiyue Feng, Meng Wang, Zhifang Chai, and Ruifang Zhao
- Subjects
biology ,Firmicutes ,Materials Science (miscellaneous) ,technology, industry, and agriculture ,Public Health, Environmental and Occupational Health ,Bacteroidetes ,02 engineering and technology ,010501 environmental sciences ,Gut flora ,021001 nanoscience & nanotechnology ,biology.organism_classification ,medicine.disease ,01 natural sciences ,Intestinal epithelium ,Microbiology ,Toxicity ,medicine ,Ingestion ,Colitis ,0210 nano-technology ,Safety, Risk, Reliability and Quality ,Safety Research ,Bacteria ,0105 earth and related environmental sciences - Abstract
Nanotechnology offers significant potential benefits to many fields, including food and agricultural industries, which have opportunities to provide products to the general public that could result in ingested nanoparticles (NPs). Herein, we explored the possible impacts of orally administered TiO 2 NPs, SiO 2 NPs and AgNPs on gut microbiota composition and colitis induction in mice. We administered the NPs to mice at doses of 2.5 mg/kg bw/day for 7 days, which was a dose relevant to human dietary intake for TiO 2 NPs and SiO 2 NPs or the acute oral exposure following medical oral application of AgNPs. No overall toxicity was recorded in mice exposed to TiO 2 NPs and SiO 2 NPs except increased pro-inflammatory cytokine levels (IL-1β, IL-6 and TNF-α) in the colons of mice that ingested SiO 2 NPs. The colitis-like symptoms induced in the mice that ingested AgNPs included high disease activity index and histological scores, disruption of microvilli and tight junctions in the intestinal epithelium, and up-regulation of pro-inflammatory cytokines. Gene analysis by 16S rRNA sequencing showed that AgNP ingestion induced shifts in the intra- and inter-phyla abundance of Bacteroidetes and Firmicutes , reduced the Firmicutes / Bacteroidetes ratio, increased the lowly abundant families of bacteria, and decreased the probiotic bacteria genus Lactobacillus . These results are similar to those reported in studies of other intestinal inflammatory disorders. SiO 2 NP ingestion in mice increased microbial species richness and diversity within the intestinal tract, and in particular, an obvious increase of the genus Lactobacillus was recorded. No obvious disturbance of gut microbiota was found in mice that ingested TiO 2 NPs. The study suggests that alterations in microbiota composition are associated with oral responses to nanoparticle exposure.
- Published
- 2017
36. Interrogating the variation of element masses and distribution patterns in single cells using ICP-MS with a high efficiency cell introduction system
- Author
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Weiyue Feng, Bing Wang, Lingna Zheng, Meng Wang, Zhifang Chai, Hanqing Chen, and Hailong Wang
- Subjects
Cell ,Population ,02 engineering and technology ,Cell fate determination ,01 natural sciences ,Biochemistry ,Mass Spectrometry ,Analytical Chemistry ,HeLa ,Single-cell analysis ,Cell Line, Tumor ,medicine ,Humans ,education ,A549 cell ,education.field_of_study ,biology ,Chemistry ,010401 analytical chemistry ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Trace Elements ,0104 chemical sciences ,Cell biology ,medicine.anatomical_structure ,Cell culture ,Cancer cell ,0210 nano-technology - Abstract
Cellular heterogeneity is an inherent condition of cell populations, which results from stochastic expression of genes, proteins, and metabolites. The heterogeneity of individual cells can dramatically influence cellular decision-making and cell fate. So far, our knowledge about how the variation of endogenous metals and non-metals in individual eukaryotic cells is limited. In this study, ICP-MS equipped with a high efficiency cell introduction system (HECIS) was developed as a method of single-cell ICP-MS (SC-ICP-MS). The method was applied to the single-cell analysis of Mn, Fe, Co, Cu, Zn, P, and S in human cancer cell lines (HeLa and A549) and normal human bronchial epithelial cell line (16HBE). The analysis showed obvious variation of the masses of Cu, Fe, Zn, and P in individual HeLa cells, and variation of Fe, Zn, and P in individual A549 cells. On the basis of the single-cell data, a multimodal distribution of the elements in the cell population was fitted, which showed marked differences among the various cell lines. Importantly, subpopulations of the elements were found in the cell populations, especially in the HeLa cancer cells. This study demonstrates that SC-ICP-MS is able to unravel the extent of variation of endogenous elements in individual cells, which will help to improve our fundamental understanding of cellular biology and reveal novel insights into human biology and medicine. Graphical abstract The variations of masses and distribution patterns of elements Mn, Fe, Co, Cu, Zn, P, and S in single cells were successfully detected by ICP-MS coupled with a high efficiency cell introduction system (HECIS).
- Published
- 2016
37. Chirality of Graphene Oxide–Humic Acid Sandwich Complex Induced by a Twisted, Long-Range-Ordered Nanostructure
- Author
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Hanqing Chen, Xiaoyan Zhou, Hailong Wang, Weiyue Feng, Kurash Ibrahim, Zhihong Li, Tu Lan, Ye Tao, Dongqi Wang, and Bing Wang
- Subjects
Circular dichroism ,Materials science ,Nanostructure ,Graphene ,Hydrogen bond ,Stacking ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Nanomaterials ,law.invention ,Crystallography ,General Energy ,Liquid crystal ,law ,Physical and Theoretical Chemistry ,0210 nano-technology ,Chirality (chemistry) - Abstract
Graphene oxide (GO) with an extraordinary atomic and electronic structure has germinated into an attractive building block for the design and fabrication of new chiral nanomaterials. Herein, we have synthesized a GO–humic acid (HA) sandwich-type complex that can self-assemble into a twisted, long-range-ordered nanostructure in aqueous media. The GO–HA sandwich complex shows obvious chirality under circular dichroism (CD) measurement. By means of scanning electron microscopy, small-angle X-ray scattering, confocal microscopy, and polarized optical microscopy (POM), the morphology, size, fine texture, and orientation of the assembly nanostructure of GO–HA have been validated. The results of experiments and molecular dynamics simulations reveal that the interactions between GO and HA (via electrostatic repulsive, π–π stacking, hydrogen bond, and solvation) induce the enhanced formation of natural ripples in a single GO–HA sheet, cause interlayer and interdomain distortion, and generate a twisted conformation...
- Published
- 2016
38. Magnetic Fe3O4 nanoparticle catalyzed chemiluminescence for detection of nitric oxide in living cells
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Huiliang Wang, Xiangzhi Zhang, Mei Li, Ibrahim Kurash, Weiyue Feng, Meng Wang, and Bing Wang
- Subjects
Inorganic chemistry ,Oxide ,02 engineering and technology ,Nitric Oxide ,010402 general chemistry ,Photochemistry ,Sensitivity and Specificity ,01 natural sciences ,Biochemistry ,Catalysis ,Cell Line ,Analytical Chemistry ,Nitric oxide ,law.invention ,Mice ,chemistry.chemical_compound ,Electron transfer ,X-ray photoelectron spectroscopy ,law ,Animals ,Magnetite Nanoparticles ,Chemiluminescence ,Superoxide ,Reproducibility of Results ,021001 nanoscience & nanotechnology ,Molecular Imaging ,0104 chemical sciences ,chemistry ,Luminescent Measurements ,Microglia ,0210 nano-technology ,Peroxynitrite - Abstract
Direct and real-time measurement of nitric oxide (NO) in biological media is very difficult due to its transient nature. Fe3O4 nanoparticles (nanoFe3O4) because of their unique catalytic activities have attracted much attention as catalysts in a variety of organic and inorganic reactions. In this work, we have developed a magnetic Fe3O4 nanoparticle-based rapid-capture system for real-time detection of cellular NO. The basic principle is that the nanoFe3O4 can catalyze the decomposition of H2O2 in the system to generate superoxide anion (O2 ·−) and the O2 ·− can serve as an effective NO· trapping agent yielding peroxynitrite oxide anion, ONOO−. Then the concentration of NO in cells can be facilely determined via peroxynitrite-induced luminol chemiluminescence. The linear range of the method is from 10−4 to 10−8 mol/L, and the detection of limit (3σ, n = 11) is as low as 3.16 × 10−9 mol/L. By using this method, the NO concentration in 0.1 and 0.5 mg/L LPS-stimulated BV2 cells was measured as 4.9 and 11.3 μM, respectively. Surface measurements by synchrotron X-ray photoelectron spectroscopy (SRXPS) and scanning transmission X-ray microscopy (STXM) demonstrate the catalytic mechanism of the nanoFe3O4-based system is that the significantly excess Fe(II) exists on the surface of nanoFe3O4 and mediates the rapid heterogeneous electron transfer, thus presenting a new Fe2O3 phase on the surface.
- Published
- 2016
39. Size-Dependent Translocation Pattern, Chemical and Biological Transformation of Nano- and Submicron-Sized Ferric Oxide Particles in the Central Nervous System
- Author
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Huiliang Wang, Hanqing Chen, Jing Zhang, Hailong Wang, Xiaoyan Zhou, Qiang Wang, Bing Wang, and Weiyue Feng
- Subjects
Male ,0301 basic medicine ,Central nervous system ,Inorganic chemistry ,Kinetics ,Biomedical Engineering ,Nanoparticle ,Bioengineering ,Chromosomal translocation ,Ferric Compounds ,Models, Biological ,Mice ,Structure-Activity Relationship ,03 medical and health sciences ,0302 clinical medicine ,Nano ,medicine ,Animals ,General Materials Science ,Particle Size ,Biotransformation ,Chemistry ,Brain ,Biological Transport ,General Chemistry ,Condensed Matter Physics ,Microspheres ,Olfactory bulb ,Nasal Mucosa ,030104 developmental biology ,medicine.anatomical_structure ,Biophysics ,Nanoparticles ,Particle ,Particle size ,030217 neurology & neurosurgery - Abstract
The present study investigated the size-dependent translocation pattern and biological fate of intranasally instilled nano- and submicron-sized Fe2O3 particles (40 nm and 280 nm) in the CNS. The particle translocation in different parts of brain at 4 h, 12 h, 24 h, 3 d, 7 d, and 30 d after intranasal instillation were quantified using ICP-MS method. A biexponential model (correlation coefficient r = 0.98-0.99) was satisfactory to describe the particokinetic translocation behavior of Fe2O3 nanoparticles in brain. We found a size-dependent translocation pattern and a time-dependent translocation mode for nano- and submicron-sized Fe2O3 nanoparticles in the olfactory bulb, which are most significant in toxic concerns of nanoparticles in the CNS. The TEM images showed particle-like substances of approximately 35-50 nm were located in the axons of olfactory neurons and in the mitochondria and lysosomes of hippocampus cells in the 40 nm-Fe2O3 exposed mice. The synchrotron-based near-edge X-ray absorption spectroscopy (XANES) was used to identify the chemical forms of the nanoparticles in brain. The XANES results indicate that the presence of chemical speciation of the Fe2O3 nanoparticle (-17%) and protein-complex like apotransferrin-Fe2O3 (-16%) in the olfactory bulb, implying that self-coating of Fe2O3 nanoparticles with transferrin occurred in brain. All the findings suggest size-sensitive manners of nano- and submicron-sized Fe2O3 particles in the brain; the smaller one possesses evident detention properties in the CNS versus the larger one.
- Published
- 2016
40. Hepatic impacts of gold nanoparticles with different surface coatings as revealed by assessing the hepatic drug-metabolizing enzyme and lipid homeostasis in mice
- Author
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Runxuan Chu, Hongyang Yu, Xue Li, Meilin Zhu, Wei Chen, Shuang Zhou, Meng Wang, Bing Wang, Weiyue Feng, Hanqing Chen, Zhifang Chai, and Lingna Zheng
- Subjects
Liver injury ,chemistry.chemical_classification ,biology ,Materials Science (miscellaneous) ,Public Health, Environmental and Occupational Health ,Cytochrome P450 ,02 engineering and technology ,010501 environmental sciences ,Pharmacology ,021001 nanoscience & nanotechnology ,medicine.disease ,01 natural sciences ,Enzyme ,chemistry ,Detoxification ,Lipogenesis ,medicine ,biology.protein ,Steatosis ,0210 nano-technology ,Safety, Risk, Reliability and Quality ,Cytotoxicity ,Safety Research ,PI3K/AKT/mTOR pathway ,0105 earth and related environmental sciences - Abstract
Liver is the major organ in the metabolization of drugs, and detoxification of exogenous substances, including nanoparticles. In clinical medical practice, drug-induced liver injury (DILI) is the major cause of acute liver failure (ALF). In this study, we explore the possible impact of intravenously injected citrate acid (CA), chitosan (CS) and polyethyleneglycol (PEG) functionalized gold nanoparticles (GNPs) on the liver of mice at the doses (60 and 120 μg/mouse) relevant to their biomedical applications. Overall, the results provide the evidence that all the GNPs did not induce obvious liver injury and hepatic steatosis in mice. However, the study highlights that alteration in the function of drug-metabolizing enzymes in the HepaRG cells was associated with the increase cytotoxicity of GNP treatments. Moreover, the deposited GNPs in the liver disturb the expression of drug-metabolizing enzymes, including drug uptake and efflux transporters, and cytochrome P450 (CYP450) isoforms and phase II metabolized enzymes. The mechanism of GNP-induced hepatotoxicity in mice is associated with hyperactivation of mTOR signalling and SREBP-1c-mediated de novo lipogenesis with hepatic inflammation. This also suggests that when using GNPs for biomedical applications, it is necessary to consider the influence of nanomaterial-liver interaction and nanomaterial-induced hepatotoxicity on drug-metabolizing enzymes and lipid homeostasis.
- Published
- 2020
41. In vivo pharmacokinetics, transfer and clearance study of graphene oxide by La/Ce dual elemental labelling method
- Author
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Shanshan Liang, Zhifang Chai, Bing Wang, Lingna Zheng, Hongyang Yu, Runxuan Chu, Meng Wang, Weiyue Feng, Xue Li, Shuang Zhou, and Hanqing Chen
- Subjects
Polyvinylpyrrolidone ,Chemistry ,Graphene ,Materials Science (miscellaneous) ,Public Health, Environmental and Occupational Health ,02 engineering and technology ,Mononuclear phagocyte system ,010501 environmental sciences ,021001 nanoscience & nanotechnology ,01 natural sciences ,law.invention ,Nanomaterials ,law ,Transmission electron microscopy ,In vivo ,Biophysics ,medicine ,Glomerular Filtration Barrier ,Distribution (pharmacology) ,0210 nano-technology ,Safety, Risk, Reliability and Quality ,Safety Research ,0105 earth and related environmental sciences ,medicine.drug - Abstract
Understanding the pharmacokinetics, distribution, and the behavior underlying the transfer and clearance of graphene oxide (GO) in vivo is critical for its clinical translation. However, GO is a single-atomic layered nanomaterial comprised only of carbon, hydrogen, and oxygen molecules, tracking GO in vivo is difficult. In this study, we developed a novel dual-element labelling strategy using the two rare-earth elements lanthanum (La) and cerium (Ce) as tags on polyvinylpyrrolidone (PVP) modified GO (La/Ce-GO-PVP) to successfully track the bioaccumulation, transfer, and clearance of GO in vivo. The data showed that intravenously injected La/Ce-GO-PVP nanosheets could be rapidly intercepted by the mononuclear phagocyte system rich organs (lung, liver, and spleen) and the kidney. A visual exploration for La/Ce-GO-PVP transfer and clearance in intra-organs and ultrastructure of the organs by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) and transmission electron microscopy (TEM) was performed. The thin, flexible, and well-dispersed GO-PVP was observed to enter into the deep sub-organs of the lung, liver and spleen, pass through the glomerular filtration barrier (GFB) of the kidney and was excreted in the urine. In addition, we observed the GO nanosheets by rolling, crumpling or folding their morphology to facilitate their transfer across the continuous, fenestrated, and discontinuous endothelium, where GO can then be transferred and cleared in the intra-organs. This study provides a further understanding for the thin and flexible GO transfer and clearance in vivo, and the interactions with physiological barriers in view of their potential biomedical applications.
- Published
- 2020
42. Concentration of chromium in whole blood and erythrocytes showed different relationships with serum apolipoprotein levels in Cr(VI) exposed subjects
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Guiping Hu, Feizai Ha, Huimin Feng, Shanfa Yu, Weiyue Feng, Zhang Ji, Yonghua Wu, Guang Jia, and Tiancheng Wang
- Subjects
0301 basic medicine ,Adult ,Chromium ,Male ,medicine.medical_specialty ,Erythrocytes ,Apolipoprotein B ,chemistry.chemical_element ,010501 environmental sciences ,01 natural sciences ,Biochemistry ,Inorganic Chemistry ,03 medical and health sciences ,chemistry.chemical_compound ,Young Adult ,Internal medicine ,medicine ,Homeostasis ,Humans ,Hexavalent chromium ,0105 earth and related environmental sciences ,Whole blood ,biology ,Chemistry ,Metabolism ,Middle Aged ,030104 developmental biology ,Endocrinology ,Apolipoproteins ,Cross-Sectional Studies ,biology.protein ,Molecular Medicine ,lipids (amino acids, peptides, and proteins) ,Female - Abstract
To explore the effects of hexavalent chromium [Cr(VI)] on apolipoprotein(Apo) metabolism.One hundred and six long-term Cr(VI) exposed subjects and fifty five non-exposed individuals were recruited. The blood metals, glucose and lipid parameters were measured to evaluate the effects of hexavalent chromium on the apolipoproteins.The quantity and homeostasis of multi-elements were significantly different between Cr(VI)exposed subjects and the controls(P 0.05). The levels of serum TG, FPG, LDL-C, Apo AI, AII, CII, CIII, E and Lp(a) also changed significantly (P 0.05). Concentration of chromium in whole blood and erythrocytes had different relationships with serum apolipoprotein levels in Cr(VI)exposed subjects.Exposure of hexavalent chromium could significantly change the homeostasis of multi-elements as well as the levels of serum apolipoproteins. Cr(III) and Cr(VI)may have different effects on the apolipoproteins.
- Published
- 2018
43. Transferrin Adsorbed on PEGylated Gold Nanoparticles and Its Relevance to Targeting Specificity
- Author
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Xinyi Wang, Chunying Chen, Mingzhe Wang, Chengxu Cai, Bing Wang, Hanqing Chen, Jian Chen, and Weiyue Feng
- Subjects
chemistry.chemical_classification ,Materials science ,technology, industry, and agriculture ,Biomedical Engineering ,Nanoparticle ,Bioengineering ,02 engineering and technology ,General Chemistry ,Polyethylene glycol ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Adsorption ,chemistry ,Dynamic light scattering ,Colloidal gold ,Transferrin ,030220 oncology & carcinogenesis ,PEG ratio ,Surface modification ,General Materials Science ,0210 nano-technology ,Nuclear chemistry - Abstract
Polyethylene glycol (PEG) has been frequently used for surface modification of nanoparticles (NPs) to reduce non-specific binding of proteins on NPs. The investigation of protein absorption on PEGylated nanoparticles is necessary. In the work, the conjugation of transferrin (Tf) to PEGylated AuNPs via adsorption or bonding was studied. The 13 nm AuNPs were coated with various molecular weight (300, 2000, 5000) carboxyl and methoxy PEG thiol. The presence of Tf on PEGylated AuNP was characterized by dynamic light scattering (DLS) and infrared spectroscopy (IR). The data of IR confirmed the presence of Tf on PEGylated AuNPs. The diameter decrease of PEGylated AuNPs after Tf adsorption was observed by DLS measurement, which is attributed to competitive adsorption between Tf and PEG molecules. These phenomena may be important to the preservation of Tf targeting specificity on PEGylated AuNPs.
- Published
- 2018
44. Thermal Unfolding Process of Lysozyme on PEGylated Gold Nanoparticles Reveals Length-Dependent Effects of PEG Layer
- Author
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Liming Wang, Mingzhe Wang, Weiyue Feng, Chengxu Cai, Chunying Chen, and Bing Wang
- Subjects
Circular dichroism ,Materials science ,Biomedical Engineering ,Bioengineering ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,0104 chemical sciences ,chemistry.chemical_compound ,chemistry ,Dynamic light scattering ,Colloidal gold ,Drug delivery ,PEG ratio ,PEGylation ,Biophysics ,General Materials Science ,Lysozyme ,Nanocarriers ,0210 nano-technology - Abstract
Characterization of bio-nano interface is crucial for developing safer and more efficient nanoparticles in nanomedical application. PEGylation is commonly used in nanocarrier for drug delivery, as it confers nanoparticles good stability, stealth effect and better targeting specificity compared with those without PEGylation. However, the protein binding state on PEGylated AuNP is still limited known. In present work, we prepared 13 nm AuNPs and then PEGylated them with thiol PEG methoxy. Lysozyme is selected as a model protein and to investigate the interactions on protein-PEGylated/AuNP interface. The thermal unfolding processes of lysozyme in absence and presence of PEGylated AuNP were measured by synchrotron radiation based circular dichroism (SRCD), dynamic light scattering (DLS) and infrared spectroscopy (IR). The results suggest that in terms of secondary structural changes, α helix content is decreased, while β sheet content is increased, and thus the adsorbed lysozyme may be present in PEG layer.
- Published
- 2018
45. Inhibition of Lysozyme Fibrillation by Gold Nanorods and Nanoparticles
- Author
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Miao Yu, Hailong Wang, Lingna Zheng, Hongli Liang, Weiyue Feng, Bing Wang, Aiping Zhang, and Meng Wang
- Subjects
Amyloid ,Materials science ,Biomedical Engineering ,Nanoparticle ,Bioengineering ,macromolecular substances ,02 engineering and technology ,010402 general chemistry ,Fibril ,01 natural sciences ,Oligomer ,chemistry.chemical_compound ,Renal Dialysis ,medicine ,General Materials Science ,Fibrillation ,Nanotubes ,Amyloidosis ,General Chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,medicine.disease ,0104 chemical sciences ,chemistry ,Diabetes Mellitus, Type 2 ,Biophysics ,Nanoparticles ,Nanorod ,Muramidase ,Gold ,medicine.symptom ,Lysozyme ,0210 nano-technology - Abstract
Amyloid fibrillation has been implicated in many neurodegenerations, dialysis-related amyloidosis, type II diabetes and more than 30 other amyloid-related diseases. Nanomaterials as potential inhibitors of amyloid fibrillation have attracted increasing interests. In the present study, the effects of gold nanorods (AuNRs) and nanoparticles (AuNPs) on amyloid fibrillation were investigated using hen egg white lysozyme (HEWL) as a model system. Our results indicated that AuNRs and AuNPs, especially AuNRs, present significant inhibitory effects on HEWL amyloid fibril formation during all the kinetic processes, from nucleation to elongation and equilibration stages. The stronger adsorption capacity of HEWL on AuNRs surface is the key mechanism of inhibition of HEWL amyloid fibrillation. Furthermore, AuNRs lead to more stable α-helix conformation and hydrophobic microenvironment of aromatic side groups in HEWL molecules, which facilitate the system to form small amorphous aggregates rather than oligomer, profibril or mature fibril.
- Published
- 2018
46. Coculture with Low-Dose SWCNT Attenuates Bacterial Invasion and Inflammation in Human Enterocyte-like Caco-2 Cells
- Author
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Hanqing Chen, Lingna Zheng, Ming Guan, Weiyue Feng, Xiaoyan Zhou, Yuliang Zhao, Zhifang Chai, Di Gao, Ruifang Zhao, and Bing Wang
- Subjects
Staphylococcus aureus ,Materials science ,Cell Survival ,Inflammasomes ,Enterocyte ,Interleukin-1beta ,Cell ,Inflammation ,medicine.disease_cause ,Bacterial Adhesion ,Epithelium ,Microbiology ,Biomaterials ,Superoxides ,NLR Family, Pyrin Domain-Containing 3 Protein ,Escherichia coli ,medicine ,Humans ,General Materials Science ,Secretion ,Viability assay ,Cytotoxicity ,Cell Shape ,Microvilli ,Nanotubes, Carbon ,Caspase 1 ,General Chemistry ,Coculture Techniques ,Mitochondria ,CARD Signaling Adaptor Proteins ,Cytoskeletal Proteins ,Enterocytes ,medicine.anatomical_structure ,Caco-2 ,Caco-2 Cells ,medicine.symptom ,Carrier Proteins ,Reactive Oxygen Species ,Biotechnology - Abstract
Single walled carbon nanotubes (SWCNTs) have been shown to be highly effective against a wide range of bacteria. Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) infection is a well-known mediator to prolong hospitalization and initiate chronic inflammation, yet the biological effects of SWCNTs on the pathogen-infected enterocytes remain unclear. Herein, it is shown that the low-dose SWCNT treatment attenuates the human enterocyte-like Caco-2 cells from the damage of E. coli and S. aureus infection by suppressing NLRP3 inflammasome activation. The relatively low-dose (1 and 10 mu g mL(-1)) SWCNT treatments reduce the adhesion and invasion of E. coli and S. aureus to Caco-2 cells, increase the cell viability and proliferation, reduce the tight junction permeability, and restitute the integrity of cell surface microvilli structure, meanwhile has low cytotoxicity to the host cells. The low-dose SWCNT treatment further reduces the NLRP3-mediated IL-1 beta secretion in the infected cells. The results identify that a low-dose SWCNT treatment serves a protective function for the E. coli- and S. aureus-infected Caco-2 cells by negatively regulating mitochondrial reactive oxygen species-mediated NLRP3 inflammasome activation.
- Published
- 2015
47. Probing the interaction at nano-bio interface using synchrotron radiation-based analytical techniques
- Author
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Yuliang Zhao, Weiyue Feng, Bing Wang, and Zhifang Chai
- Subjects
chemistry.chemical_classification ,Light source ,Materials science ,chemistry ,Bio interface ,Biomolecule ,Interface (computing) ,Nano ,High spatial resolution ,Synchrotron radiation ,Nanotechnology ,General Chemistry ,Nanomaterials - Abstract
Understanding the interactions of nanomaterials (NMs) with biomolecules, organelles, cells, and organic tissues at the nano-bio interface can offer important information for their uptake, distribution, translocation, metabolism and degradation in vitro and in vivo, which can help to precisely tune and design “smart” NMs for biomedical applications. However, probing the interactions at the nano-bio interface, which generally requires dedicated analytical methods and tools, is remarkably complicated due to the dynamically changed nature of the nano-bio interface. Because of the advantages of high spatial resolution, high sensitivity, excellent accuracy, low matrix effects and non-destructiveness, synchrotron radiation (SR)-based analytical techniques have become extremely valuable tools. Herein, we present a comprehensive overview of SR-based techniques for the visualized study of NMs at cellular and subcellular interfaces and their transformation in vitro; the exploration of biodistribution, translocation, metabolism and degradation of NMs in vivo; and clarification of the molecular mechanisms of NMs’ reactions with biomolecules. Rapid development of advanced light source means that in situ, real-time analysis of NMs at the nano-bio interface will be achieved.
- Published
- 2015
48. Quantitative analysis of Gd@C82(OH)22 and cisplatin uptake in single cells by inductively coupled plasma mass spectrometry
- Author
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Hanqing Chen, Meng Wang, Bing Wang, Bao Yun Sun, Yuliang Zhao, Ling Na Zheng, Weiyue Feng, Zhifang Chai, and Lei Chao Zhao
- Subjects
Cisplatin ,Chromatography ,biology ,Chemistry ,Antineoplastic Agents ,Gadolinium ,biology.organism_classification ,Mass spectrometry ,Biochemistry ,Mass Spectrometry ,Nanostructures ,Analytical Chemistry ,HeLa ,Single-cell analysis ,Cell culture ,Cell Line, Tumor ,Neoplasms ,medicine ,Humans ,Single-Cell Analysis ,Quantitative analysis (chemistry) ,Inductively coupled plasma mass spectrometry ,Intracellular ,medicine.drug - Abstract
Cisplatin is a commonly used chemotherapeutic drug in cancer treatment, whereas Gd@C82(OH)22 is a new nanomaterial anti-tumor agent. In this study, we determined intracellular Gd@C82(OH)22 and cisplatin after treatment of Hela and 16HBE cells by single cell inductively coupled plasma-mass spectrometry (SC-ICP-MS), which could provide quantitative information at a single-cell level. The cell digestion method validated the accuracy of the SC-ICP-MS. The concentrations of Gd@C82(OH)22 and cisplatin in cells at different exposure times and doses were studied. The SC-ICP-MS is a promising complement to available methods for single cell analysis and is anticipated to be applied further to biomedical research.
- Published
- 2015
49. Facile Approach To Observe and Quantify the αIIbβ3 Integrin on a Single-Cell
- Author
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Xueyun Gao, Meng Wang, Lina Zhao, Lingna Zheng, Yuliang Zhao, Fuping Gao, Zhifang Chai, Weiyue Feng, Liang Gao, Jiao Zhai, Yaling Wang, Ru Liu, and Chao Xu
- Subjects
Blood Platelets ,Integrin ,Cell ,Peptide ,Platelet Glycoprotein GPIIb-IIIa Complex ,Analytical Chemistry ,law.invention ,Confocal microscopy ,law ,Mole ,Tumor Cells, Cultured ,medicine ,Humans ,chemistry.chemical_classification ,Microscopy, Confocal ,biology ,Chemistry ,Cell Membrane ,αiibβ3 integrin ,Molecular biology ,Fluorescence ,Peptide Fragments ,Membrane ,medicine.anatomical_structure ,biology.protein ,Biophysics ,Gold ,Leukemia, Erythroblastic, Acute ,Single-Cell Analysis - Abstract
We report for the first time seeing and counting integrin α(IIb)β3 on a single-cell level. The proposed method is based on the using of the Au cluster probe. With the fluorescent property of Au24 cluster and the specific targeting ability of peptide, our probe can directly visualize integrin α(IIb)β3 on the membrane of human erythroleukemia cells (HEL) via confocal microscopy. On the basis of the accurate formula of our probe (Au24Peptide8), the number of integrin α(IIb)β3 can be precisely counted by quantifying the gold content on a single HEL cell via laser ablation inductively coupled plasma mass spectrometry. Our results reveal that the number of integrin α(IIb)β3 on a single cell varies from 5.75 × 10(-17) to 9.11 × 10(-17) mol, because of the heteroexpression levels of α(IIb)β3 on individual cells.
- Published
- 2015
50. Structure and catalytic activities of ferrous centers confined on the interface between carbon nanotubes and humic acid
- Author
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Hanqing Chen, Xiaoyan Zhou, Jing Zhang, Bing Wang, Jun-Jie Yin, Dongqi Wang, Weiyue Feng, Xingfa Gao, Kurash Ibrahim, Yuliang Zhao, and Zhifang Chai
- Subjects
Iron ,Normal Distribution ,chemistry.chemical_element ,Electrons ,Carbon nanotube ,Ligands ,Catalysis ,Phenolsulfonphthalein ,Ferrous ,law.invention ,Electron transfer ,Phenols ,Transition metal ,law ,Humic acid ,General Materials Science ,Benzhydryl Compounds ,Humic Substances ,Ions ,chemistry.chemical_classification ,Nanotubes, Carbon ,Chemistry ,Electron Spin Resonance Spectroscopy ,Water ,Aromaticity ,Hydrogen Peroxide ,Carbon ,Oxygen ,Chemical engineering ,Metals ,Physical chemistry ,Adsorption - Abstract
Preparation of heterogeneous catalysts with active ferrous centers is of great significance for industrial and environmental catalytic processes. Nanostructured carbon materials (NCM), which possess free-flowing p electrons, can coordinate with transition metals, provide a confinement environment for catalysis, and act as potential supports or ligands to construct analogous complexes. However, designing such catalysts using NCM is still seldom studied to date. Herein, we synthesized a sandwich structured ternary complex via the coordination of Fe-loaded humic acid (HA) with C=C bonds in the aromatic rings of carbon nanotubes (CNTs), in which the O/N-Fe-C interface configuration provides the confinement environment for the ferrous sites. The experimental and theoretical results revealed octahedrally/tetra-hedrally coordinated geometry at Fe centers, and the strong hybridization between CNT C pi* and Fe 3d orbitals induces discretization of the atomic charges on aromatic rings of CNTs, which facilitates O-2 adsorption and electron transfer from carbon to O-2, which enhances O-2 activation. The O-2 activation by the novel HA/Fe-CNT complex can be applied in the oxidative degradation of phenol red (PR) and bisphenol A (BPA) in aqueous media.
- Published
- 2015
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