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6 results on '"Weissinger, Eva Maria"'

2. Sequential anti-cytomegalovirus response monitoring may allow prediction of cytomegalovirus reactivation after allogeneic stem cell transplantation

Author

Borchers, Sylvia, Bremm, Melanie, Lehrnbecher, Thomas, Dammann, Elke, Pabst, Brigitte, Wölk, Benno, Esser, Ruth, Yildiz, Meral, Eder, Matthias, Stadler, Michael, Bader, Peter, Martin, Hans, Jarisch, Andrea, Schneider, Gisbert, Klingebiel, Thomas, Ganser, Arnold, Mischak-Weissinger, Eva Maria, Köhl, Ulrike, Borchers, Sylvia, Bremm, Melanie, Lehrnbecher, Thomas, Dammann, Elke, Pabst, Brigitte, Wölk, Benno, Esser, Ruth, Yildiz, Meral, Eder, Matthias, Stadler, Michael, Bader, Peter, Martin, Hans, Jarisch, Andrea, Schneider, Gisbert, Klingebiel, Thomas, Ganser, Arnold, Mischak-Weissinger, Eva Maria, and Köhl, Ulrike

Abstract

Background: Reconstitution of cytomegalovirus-specific CD3+CD8+ T cells (CMV-CTLs) after allogeneic hematopoietic stem cell transplantation (HSCT) is necessary to bring cytomegalovirus (CMV) reactivation under control. However, the parameters determining protective CMV-CTL reconstitution remain unclear to date. Design and Methods: In a prospective tri-center study, CMV-CTL reconstitution was analyzed in the peripheral blood from 278 patients during the year following HSCT using 7 commercially available tetrameric HLA-CMV epitope complexes. All patients included could be monitored with at least CMV-specific tetramer. Results: CMV-CTL reconstitution was detected in 198 patients (71%) after allogeneic HSCT. Most importantly, reconstitution with 1 CMV-CTL per µl blood between day +50 and day +75 post-HSCT discriminated between patients with and without CMV reactivation in the R+/D+ patient group, independent of the CMV-epitope recognized. In addition, CMV-CTLs expanded more daramtaically in patients experiencing only one CMV-reactivation than those without or those with multiple CMV reactivations. Monitoring using at least 2 tetramers was possible in 63% (n = 176) of the patients. The combinations of particular HLA molecules influenced the numbers of CMV-CTLs detected. The highest CMV-CTL count obtained for an individual tetramer also changed over time in 11% of these patients (n = 19) resulting in higher levels of HLA-B*0801 (IE-1) recognizing CMV-CTLs in 14 patients. Conclusions: Our results indicate that 1 CMV-CTL per µl blood between day +50 to +75 marks the beginning of an immune response against CMV in the R+/D+ group. Detection of CMV-CTL expansion thereafter indicates successful resolution of the CMV reactivation. Thus, sequential monitoring of CMV-CTL reconstitution can be used to predict patients at risk for recurrent CMV reactivation.

Published
2012

4. Early and Effective Immune-Recovery by Gene-Engineered Lymphocytes after Haploidentical Transplantation for Leukemia Abate Late Transplant Mortality

Author

Stanghellini, Maria Teresa Lupo, primary, Ciceri, Fabio, primary, Bonini, Chiara, primary, Bondanza, Attilio, primary, Traversari, Catia, primary, Salomoni, Monica, primary, Turchetto, Lucia, primary, Colombi, Scialini, primary, Bernardi, Massimo, primary, Peccatori, Jacopo, primary, Pescarollo, Alessandra, primary, Servida, Paolo, primary, Magnani, Zulma, primary, Perna, Serena Kimi, primary, Valtolina, Veronica, primary, Crippa, Fulvio, primary, Callegaro, Luciano, primary, Spoldi, Elena, primary, Crocchiolo, Roberto, primary, Fleischhauer, Katharina, primary, Ponzoni, Maurilio, primary, Vago, Luca, primary, Santoro, Armando, primary, Todisco, Elisabetta, primary, Apperley, Jane, primary, Olavarria, Eduardo, primary, Slavin, Shimon, primary, Weissinger, Eva Maria, primary, Hertenstein, Bernd, primary, Stadler, Michael, primary, Yannaki, Evangelia, primary, Fassas, Athanasios, primary, Anagnostopoulos, Achilles, primary, Stampino, Corrado Gallo, primary, Bregni, Marco, primary, Bruzzi, Paolo, primary, and Bordignon, Claudio, primary

Published
2008
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6. Reconstitution and Phenotype of Tregs in CMV Reactivating Patients Following Allogeneic Hematopoietic Stem Cell Transplantation.

Author

Velaga, Sarvari, Ukena, Sya N., Höpting, Matthias, Ivanyi, Philipp, Borchers, Sylvia, Mischak-Weissinger, Eva-Maria, Hamwi, Iyas, Buchholz, Stefanie, Ganser, Arnold, and Franzke, Anke

Subjects
HUMAN phenotype, HEMATOPOIETIC stem cell transplantation, CYTOMEGALOVIRUS diseases, T cells, VIRUS reactivation, GRAFT versus host disease prevention
Abstract

In experimental and clinical settings Tregs prevent graft-versus-host disease (GvHD) by inhibiting the proliferation and function of conventional T cells (Tconv). The suppressive potency of Tregs might also lead to the inhibition of protective antiviral T cell responses. As the control of CMV reactivation is important to improve the clinical outcome in allogeneic HSCT, we analyzed the Treg reconstitution in CMV reactivating patients with and without GvHD (n=47) in the first 6 months following transplantation. Most importantly, CMV reactivation does not correlate with the numerical reconstitution of CD4+CD25highCD127− Tregs. During CMV reactivation the proportion of Tregs within the CD4+ T cell population decreased significantly independent of GvHD manifestation. A comprehensive FACS analysis was performed in order to characterize the phenotype of Tregs and Tconv cells in greater detail for activation, co-stimulation, proliferation, suppressive function and migratory capability. Interestingly, Tregs of patients with CMV reactivation showed a significantly higher CXCR3 expression. CD4+ Tconv cells expressed significantly higher protein levels of the proliferation marker Ki67 correlating with a numerical increase of CD4+ T cells. Our results indicate that Tregs are not inhibiting pathogen clearance by Tconv following HSCT, which is of high relevance for future Treg cell-based clinical trials in allogeneic HSCT. [ABSTRACT FROM AUTHOR]

Published
2013
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