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Clustered, regularly interspaced, short palindromic repeats-CRISPR associated (CRISPR-Cas) systems defend bacteria against foreign nucleic acids, such as during bacteriophage infection and transformation, processes which cause envelope stress. It is unclear if these machineries enhance membrane integrity to combat this stress. Here, we show that the Cas9-dependent CRISPR-Cas system of the intracellular bacterial pathogen Francisella novicida is involved in enhancing envelope integrity through the regulation of a bacterial lipoprotein. This action ultimately provides increased resistance to numerous membrane stressors, including antibiotics. We further find that this previously unappreciated function of Cas9 is critical during infection, as it promotes evasion of the host innate immune absent in melanoma 2/apoptosis associated speck-like protein containing a CARD (AIM2/ASC) inflammasome. Interestingly, the attenuation of the cas9 mutant is complemented only in mice lacking both the AIM2/ASC inflammasome and the bacterial lipoprotein sensor Toll-like receptor 2, but not in single knockout mice, demonstrating that Cas9 is essential for evasion of both pathways. These data represent a paradigm shift in our understanding of the function of CRISPR-Cas systems as regulators of bacterial physiology and provide a framework with which to investigate the roles of these systems in myriad bacteria, including pathogens and commensals.

We present a scheme for correcting qubit loss error while quantum computing with neutral atoms in an addressable optical lattice. The qubit loss is first detected using a quantum nondemolition measurement and then transformed into a standard qubit error by inserting a new atom in the vacated lattice site. The logical qubit, encoded here into four physical qubits with the Grassl-Beth-Pellizzari code, is reconstructed via a sequence of one projective measurement, two single-qubit gates, and three controlled-NOT operations. No ancillary qubits are required. Both quantum nondemolition and projective measurements are implemented using a cavity quantum electrodynamics system which can also detect a general leakage error and thus allow qubit loss to be corrected within the same framework. The scheme can also be applied in quantum computation with trapped ions or with photons.