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Abstract Osteotomy can correct kyphosis, restore the spinal sequence, and restore the healthy appearance of a patient. However, the aorta is stretched during pedicle subtraction osteotomy (PSO), and some surgeons are concerned about aortic injury. We used finite element analysis to construct an aortic model to simulate hemodynamic changes during osteotomy. 16 patients with ankylosing spondylitis kyphosis who had undergone a two-level osteotomy at the L1 and L3 levels was included in this study. Aortic computed tomography angiography (CTA) was performed, and a 3D image model was constructed. The length, transverse diameter, and curvature of the aorta were used to evaluate morphological changes. Finite element analysis was used to analyze the changes in aortic fluid dynamics. Blood pressure, wall shear stress, and blood flow velocity were compared pre- and postoperatively. The overall length of the aorta before surgery was 424.3 ± 42.9 mm, and the overall length of the aorta after surgery was 436.2 ± 54.8 mm. The aortic curvature decreased from 0.27 ± 0.13 to 0.17 ± 0.09. The mean transverse diameter of the aorta did not change (19.3 ± 6.6 vs. 19.2 ± 7.4 mm, P > 0.05). The blood flow velocity (2.8 ± 1.1 vs. 1.5 ± 0.8 m/s, P

Abstract Diabetes mellitus (DM) is a well-documented risk factor of intervertebral disc degeneration (IVDD). The current study was aimed to clarify the effects and mechanisms of NADH: ubiquinone oxidoreductase subunit A3 (NDUFA3) in human nucleus pulposus cells (HNPCs) exposed to high glucose. NDUFA3 was overexpressed in HNPCs via lenti-virus transduction, which were co-treated with high glucose and rotenone (a mitochondrial complex I inhibitor) for 48 h. Cell activities were assessed for cell viability, cell apoptosis, reactive oxygen species (ROS) production, mitochondrial membrane potential (MMP) ratio, oxygen consumption rate (OCR) and mitochondrial complexes I activities. High glucose decreased cell viability, increased apoptotic cells, increased ROS production, decreased MMP levels and OCR values in HNPCs in a dose-dependent manner. Rotenone co-treatment augmented the high glucose-induced injuries on cell viability, apoptosis, ROS production and mitochondrial function. NDUFA3 overexpression counteracted the high glucose-induced injuries in HNPCs. HDAC/H3K27ac mechanism was involved in regulating NDUFA3 transcription. NDUFA3 knockdown decreased cell viability and increased apoptotic cells, which were reversed by ROS scavenger N-acetylcysteine. HDAC/H3K27ac-mediated transcription of NDUFA3 protects HNPCs against high glucose-induced injuries through suppressing cell apoptosis, eliminating ROS, improving mitochondrial function and oxidative phosphorylation. This study sheds light on candidate therapeutic targets and deepens the understanding of molecular mechanisms behind DM-induced IVDD.

Objective The pedicle screw technique has been widely used in adolescent idiopathic scoliosis orthopedic surgery, but misplacement of screws may damage important structures such as blood vessels and nerves around the pedicle, resulting in serious consequences. Therefore, our research team has independently developed a surgical tool to assist in the placement of pedicle screws. This study aims to investigate the safety and accuracy of postural awareness tool assisted nail placement in orthopedic surgery for adolescent idiopathic scoliosis. Method A retrospective analysis was performed on 24 adolescent patients with idiopathic scoliosis admitted to our hospital from July 2019 to July 2022, including 10 males and 14 females, with an average age of 14.88 ± 2.36 years (10–19 years). The mean follow‐up was 15.67 ± 2.20 months (12–20 months). We divided the patients into postural awareness group (n = 12) and C‐arm group (n = 12) according to whether the postural awareness surgical tool was used during the operation. All patients were treated with posterior spinal orthopedic surgery. The postural awareness group was assisted by pedicle screw placement with a postural awareness surgical tool, while the C‐arm group was given a pedicle screw placement with freehand technique. The operative time, intraoperative blood loss, intraoperative fluoroscopy times, nail placement related complications, nail placement accuracy, and scoliosis correction rate were recorded and compared between the two groups. Results The operative time, intraoperative blood loss and fluoroscopy times in the postural awareness group were significantly lower than those in the C‐arm group, with statistical significance (p

Objective: This study summarized and analyzed the clinical data, preoperative preparation, surgical process and postoperative recovery of patients, to explore the clinical effect of posture-aware surgical tool in adolescent idiopathic scoliosis orthopedic surgery. Methods: This study demonstrated the whole process of using posture-aware surgical tools to assist the placement of screws in orthopedic surgery of adolescent idiopathic scoliosis. A 14-year-old and 8-month-old male with height 166 ​cm and weight 60 ​kg was diagnosed as Lenke type 1A adolescent idiopathic scoliosis. The preoperative X-ray films of the patients showed that the Cobb angle was 66°, CSVL-C7PL (Central Sacral Vertical Line -C7 plumb line) was 4 ​mm, SVA (sagittal vertical axis) was 5 ​mm. The operative fixed segment was T5∼L3 vertebral body. Renaissance and other three-dimensional software were utilized for screw placement angle, entry point and other related information before operation. The use of posture-aware surgical tools during surgery can accurately select screw length, type and screw placement angle, as planned by using three-dimensional software before operation. Results: The entire operation process was smooth. The intraoperative blood loss was about 600 ​ml, and the operation time was about 445 ​min. A total of 18 screws were placed during the whole operation.The patient's Cobb Angle was 8.94°, CSVL-C7PL was 2 ​mm, and SVA was 3 ​mm on postoperative radiographs. Conclusion: The implantation of pedicle screw assisted by posture-aware surgical tool is safe and effective with a smooth operation process and favorable patient outcomes.

Objective: To investigate the accuracy and repeatability of the software that we developed in measuring the adolescent idiopathic scoliosis (AIS) Cobb angle. Methods: Fifty patients with AIS were included in the study. Three independent surveyors measured the Cobb angle of coronal main curve, thoracic kyphosis angle (TK), thoracolumbar kyphosis angle (TLK) and lumbar kyphosis angle (LL) by using protractor, scoliosis screening software and picture archiving and communication system (PACS). The measurement was repeated after an interval of 2 weeks. The measurement time of software method and protractor method were compared by Paired t-test. Taking the Cobb angle measurement results of the PACS as the reference standard. The repeatability of the surveyor and the consistency between the surveyors are compared by the intraclass correlation coefficient (ICC). Results: Among the 50 patients, there were 18 males and 32 females, aged from 10 to 18 years (mean age of 14.4 years). The time of measuring Cobb angle by software method was significantly shorter than that by protractor method (94.7 ​± ​8.5s versus 217.9 ​± ​24.1s, t ​= ​−60.536, P ​ ​0.05). The reliability analysis showed that the three surveyors used software to measure the Cobb angle of the coronal main curve, TK, TLK and LL were all in good consistency (ICC>0.90). The repeatability and consistency of the software method used by the three surveyors were higher than that of the protractor method (ICC> 0.90). Conclusions: Compared with traditional protractor, the smartphone scoliosis screening software has shorter time, higher efficiency, higher accuracy and repeatability in measuring Cobb angle of AIS, so as to widely screen the diagnosis of scoliosis patients.

Introduction. This study aimed to compare and analyze the effect of preoperative zoledronic acid (ZOL) administration on pain intensity after percutaneous vertebroplasty (PVP) for osteoporotic vertebral compression fracture (OVCF). Methods. The study included 242 patients with OVCFs who underwent PVP in our hospital between January 2015 and June 2018. The patients were randomly assigned to either a ZOL group (n = 121) or a control group (n = 121). The patients in the ZOL group were treated preoperatively with intravenous infusion of 5 mg ZOL. Those in the control group were treated without ZOL. All the patients were followed up for 1 year. Results. No statistically significant differences in age, sex, weight, and body mass index (BMI) were found between the two groups. During the follow-up period, the visual analog scale score and Oswestry dysfunction index score in the ZOL group were lower than those in the control group. The bone mineral density at 6 or 12 months after treatment was significantly higher and the levels of the bone metabolism markers were significantly lower in the ZOL group than in the control group (P

Abstract Background Acute respiratory distress syndrome (ARDS) after cardiac surgery is a severe respiratory complication with high mortality and morbidity. Traditional clinical approaches may lead to under recognition of this heterogeneous syndrome, potentially resulting in diagnosis delay. This study aims to develop and external validate seven machine learning (ML) models, trained on electronic health records data, for predicting ARDS after cardiac surgery. Methods This multicenter, observational cohort study included patients who underwent cardiac surgery in the training and testing cohorts (data from Nanjing First Hospital), as well as those patients who had cardiac surgery in a validation cohort (data from Shanghai General Hospital). The number of important features was determined using the sliding windows sequential forward feature selection method (SWSFS). We developed a set of tree-based ML models, including Decision Tree, GBDT, AdaBoost, XGBoost, LightGBM, Random Forest, and Deep Forest. Model performance was evaluated using the area under the receiver operating characteristic curve (AUC) and Brier score. The SHapley Additive exPlanation (SHAP) techinque was employed to interpret the ML model. Furthermore, a comparison was made between the ML models and traditional scoring systems. ARDS is defined according to the Berlin definition. Results A total of 1996 patients who had cardiac surgery were included in the study. The top five important features identified by the SWSFS were chronic obstructive pulmonary disease, preoperative albumin, central venous pressure_T4, cardiopulmonary bypass time, and left ventricular ejection fraction. Among the seven ML models, Deep Forest demonstrated the best performance, with an AUC of 0.882 and a Brier score of 0.809 in the validation cohort. Notably, the SHAP values effectively illustrated the contribution of the 13 features attributed to the model output and the individual feature's effect on model prediction. In addition, the ensemble ML models demonstrated better performance than the other six traditional scoring systems. Conclusions Our study identified 13 important features and provided multiple ML models to enhance the risk stratification for ARDS after cardiac surgery. Using these predictors and ML models might provide a basis for early diagnostic and preventive strategies in the perioperative management of ARDS patients.

Abstract Adult tendon stem/progenitor cells (TSPCs) are essential for tendon maintenance, regeneration, and repair, yet they become susceptible to senescence with age, impairing the self-healing capacity of tendons. In this study, we employ a recently developed deep-learning-based efficacy prediction system to screen potential stemness-promoting and senescence-inhibiting drugs from natural products using the transcriptional signatures of stemness. The top-ranked candidate, prim-O-glucosylcimifugin (POG), a saposhnikovia root extract, could ameliorate TPSC senescent phenotypes caused by long-term passage and natural aging in rats and humans, as well as restore the self-renewal and proliferative capacities and tenogenic potential of aged TSPCs. In vivo, the systematic administration of POG or the local delivery of POG nanoparticles functionally rescued endogenous tendon regeneration and repair in aged rats to levels similar to those of normal animals. Mechanistically, POG protects TSPCs against functional impairment during both passage-induced and natural aging by simultaneously suppressing nuclear factor-κB and decreasing mTOR signaling with the induction of autophagy. Thus, the strategy of pharmacological intervention with the deep learning-predicted compound POG could rejuvenate aged TSPCs and improve the regenerative capacity of aged tendons.

Goose meat is increasingly popular among consumers because of its good quality. The fiber characteristics have been well demonstrated to be key contributing factors of meat quality, and the marketable ages are also closely related to meat quality. However, little is known about the effect of different marketable ages on the quality of goose meat through its fiber characteristics. Here, fiber characteristics of Yangzhou geese of different marketable ages (70, 90, and 120 d) and their effect on meat quality were investigated. The results showed that only fast-twitch fibers were present in breast muscle, irrespective of age, and that few slow-twitch fibers could be identified in leg muscle, especially in gastrocnemius and extensor digitorum longus. Fiber diameter in breast muscle increased rapidly from age 70 d to 90 d, from 19.88 to 26.27 μm, and remained stable for 90 d thereafter. The diameter and cross-sectional area of muscle fiber continue to grow with day increasing in leg muscle. In addition, we measured the proximate composition and physical properties at different ages. Among the 3 marketable ages investigated, the 120-day-old geese had higher intramuscular fat and protein content, as well as lower moisture content, both in breast and leg meat. Greater lightness and pressing loss, with lower redness and shear force, were observed in the breast and leg meat of 70-day-old geese when compared with 90- or 120-day-old geese. Taken together, although older marketable age hardly affected muscle fiber type in geese, it would contribute to larger muscle fiber area, higher intramuscular fat and protein content, as well as redder and chewier meat. As a result, the reasonable marketable age should be taken into account to improve quality in goose meat production, and the marketable age of 90 or 120 d was recommended and it could potentially improve meat quality in goose meat production.

ABSTRACT: Muscle fiber characteristics had beneficial effects on meat masses and meat quality in broilers. Its number is determined at birth and directly affects the growth and development of muscle fibers after birth. However, whether the muscle fiber characteristics in different types of chickens are different at birth has not been fully documented. In this study, the 1-day-old Xueshan chicken (slow-growing broiler) and Ross 308 broiler (fast-growing broiler) were selected, respectively, and the fiber type distribution, fiber density, and fiber size in the breast (pectoralis major, PM) and leg (gastrocnemius, GAS) muscles were detected. The results showed that the PM only made up of type IIB fibers regardless of breed, and that few type I fibers (approximately 17.55%) was identified in GAS of Ross 308 broiler. The PM muscles had significantly higher fiber density, lower cross-sectional area and diameter than those of GAS muscles in both 2 breeds (P < 0.05). The highest fiber density was observed in PM of Xueshan chicken. Furthermore, the muscle fiber characteristics were partly controlled by glycolytic potential (GP), and the GP was also invesgated. The GP in PM and GAS of Ross 308 broiler were higher than in Xueshan chicken (P < 0.05). Taken together, 1-day-old Xueshan chicken exhibited higher fiber density and lower GP compared to 1-day-old Ross 308 broiler, especially in PM, which could not only reveal the differences of muscle characteristics among different types of chickens, but also explore a new way to improve the masses and quality of poultry meat.

ABSTRACT: Chicken meat is an important source of high-quality animal protein. Its consumption continues to grow in both developed and developing countries. Muscle fiber characteristics are key determinants of meat quality and quantity. Skeletal muscle is a highly plastic tissue that is affected by breed differences and muscular tissues. However, studies regarding the effects of different breeds and muscular tissues on the fibers and meat quality traits in broilers are lacking. In this study, Ross 308 chickens (fast-growing [FG] broilers) and Xueshan chickens (slow-growing [SG] broilers) were selected, and their fiber and meat quality traits were characterized. The results showed that the breast muscle primarily comprised glycolytic fibers, whereas the leg muscle comprised glycolytic and a few oxidative fibers, regardless of the breed. The highest percentage of oxidative fibers (26.51%) appeared in the soleus muscle (SOL) of SG broilers. In addition, higher shear force, lower pressing loss, and thicker muscle fibers with less extracellular space were observed for SG meat than for FG meat. When comparing the different muscular tissues, a higher oxidative fiber percentage, ultimate pH, redness, and intramuscular fat (IMF) content were detected in the leg muscle than in the breast muscle in the 2 breeds. In summary, these data indicated that SG broilers had thicker muscle fibers than the FG broilers and that the leg muscle had more oxidative fibers than the breast muscle. Thicker fibers may contribute to increased firmness and more oxidative fibers lead to higher redness value and IMF content.

ABSTRACT: Marketable age is an important determinant of meat quality. Cherry Valley duck (SM3 medium) is the most efficient Pekin-type duck and is the most widely farmed breed globally. However, whether marketable age determines the meat quality of Cherry Valley ducks is not well documented. The objective of this study was to investigate the effect of marketable age on the proximate composition and nutritional profile of breast meat from Cherry Valley broiler ducks. Ducks at 28, 38, 42, and 45 days old were selected and slaughtered, and their proximate composition, cholesterol and essential mineral compositions, and amino acids and fatty acid profile of breast meat lipid were determined. The results showed higher protein content and lower intramuscular fat content were observed in the 38-day-old ducks than in the 28-day-old birds (P < 0.05). Additionally, 38-day-old ducks contained higher Fe and Mg contents (P < 0.05), whereas 28-day-old birds had higher Zn and Ca contents (P < 0.05). The essential amino acid content in 38-day-old was about 95.29 g/kg, higher than that in 28-day-old birds (P < 0.05). The contents of C20:5 n-3, omega-6 polyunsaturated fatty acids, and polyunsaturated fatty acids were the highest in 38-day-old birds (P < 0.05), whereas the content of C20:4 n-6, DHA (C22:6 n-3), and saturated fatty acids in 28-day-old birds was the lowest (P < 0.05). Finally, a comprehensive evaluation model of multiple traits was developed by applying principal component analysis, and the meat nutrition of 38-day-old ducks was identified as the optimal. Taken together, the meat of 38-day-old ducks had an advantage in proximate composition, minerals content, essential amino acids, and fatty acids, and 38 d might be recommended as an appropriate marketable age to provide duck meat of high nutrition value.

ABSTRACT: Goose (Anas cygnoides), as a typical species domesticated from a migratory bird, has maintained the capability of depositing excess lipid and preferentially accumulating fat within the abdomen and subcutaneous, which not only leads to decrease in yield of meat product, but also affects the feed conversion rate. Here, an experiment was conducted to examine the difference in developmental dynamics between subcutaneous (SAT) and abdominal adipose tissues (AAT) in goose. The results showed that SAT could be clearly observed at embryonic days (E) 15, whereas AAT were clearer until E20. Although the weights of SAT and AAT showed a significant rising with advancing age (P < 0.05), their gains were not completely uniform, and more adipose deposited preferentially toward AAT after birth (P < 0.05). Additionally, a clear expansion in adipocyte size was observed in AAT and SAT during embryonic stages (P < 0.05). The average adipocyte area in AAT continued to increase after birth (P < 0.05), while the cell areas in SAT were relatively invariable (P > 0.05). Furthermore, the expression levels of FABP4/aP2, ACSL1 and PPARγ were much higher in SAT than in AAT, whereas relative higher expression level of IL-6 was observed in the AAT during embryonic stages. After birth, the more expression of LPL and PPARα were detected in AAT than did in SAT (P < 0.05), whereas greater ATGL expression was in SAT (P < 0.05). Taken together, these findings suggest that AAT may display greater fat storage capacity than SAT accompanied by changes in cell area and lipogenic capacity. Considering that there is disparity in the individual adipose tissues, we suggested that careful consideration for the precise interventions used to control SAT or AAT deposition in meat-producing animals to improve feed efficiency.

ABSTRACT: This study evaluated the effects of muscle fiber characteristics on meat quality traits in 45 female fast- and slow-growing ducks. Three duck breeds at typical market ages were selected and slaughtered, including fast-growing ducks (Cherry Valley duck) and slow-growing ducks (Small-sized Beijing duck and Liancheng White duck). M. pectoralis major (PM), m. soleus (SOL), m. gastrocnemius (GAS) and m. extensor digitorum longus (EDL) were used to assess muscle fiber characteristics as well as meat quality properties. The results showed that the fiber compositions in PM, GAS, and EDL muscles only consisted of fast-twitch fibers irrespective of the breeds, while a low percentage of slow-twitch fibers were observed in slow-growing ducks (17.03% and 29.14%). The significant clear differences of fiber diameter, fiber density and fiber cross-sectional area (CSA) was observed among three duck breeds. Small-sized Beijing ducks had the highest diameter and cross-sectional fiber area coupled with a dramatically lowest fiber density when compared to other 2 breeds both in breast and leg muscles. In addition, the meat quality traits such as moisture content, release water, and intramuscular fat content were significantly affected by the breeds. Slow-growing ducks, especially Liancheng White ducks, exhibited higher release water, intramuscular fat content, as well as lower moisture content (P < 0.05) compared to the fast-growing ducks. The lower pH24 h value and shear force tended to be present in breast of Liancheng White ducks (P < 0.05). The higher protein content and collagen content were detected in breast of Liancheng White ducks and the leg muscle of Small-sized Beijing ducks (P < 0.05), respectively. Finally, the correlation coefficients between muscle fiber characteristics and meat quality showed that the diameter, density and CSA of fibers had a moderate or significant correlation with pH, shear force value, moisture content, and protein content of meat in fast-growing ducks. In slow-growing ducks, muscle fiber characteristics had a moderate or significant correlation with pH, shear force value, release water, protein content, and intramuscular fat content of meat. These results indicated that muscle fiber characteristics is a useful parameter to explain in parts the variation of meat quality including pH, shear force value, and protein content of meat, both in slow-growing ducks and fast-growing ducks.

ABSTRACT: Integrated rice-duck farming (RD) system, which aims to improve the welfare of ducks, has gained popularity in Asian countries. However, the effects of RD system on the carcass and quality traits of duck meat have not been evaluated. Here, a paddy field experiment was conducted to examine the effects of RD system on the carcass and quality traits of duck meat. A total of 180 Jinding ducklings (7 days old) were randomly divided into 2 rearing systems of floor pen rearing (FPR) system and RD system. After 11 weeks, 12 ducks from each rearing system (6 males and females each) were used for carcass traits, meat quality, amino acid, and fatty acid analyses. The results showed that ducks reared in the RD system had higher carcass yield and intramuscular fat content (P < 0.05) than those reared in the FPR system; however, ducks reared in the RD system had lower protein and moisture content (P < 0.05). Additionally, the concentration of essential amino acids, including Tyr, Val, Met, Phe, His, Ala, Arg, and Pro, was higher in the breast muscle of ducks reared in the RD system than those reared in the FPR system. Furthermore, higher saturated fatty acid (C12:0, C14:0, C16:0, C18:0, and C21:0), monounsaturated fatty acid (C16:1, C18:1, and C18:1T), and polyunsaturated fatty acid (C22:2, C18:2n-6, and C22:6n3) content was recorded in the breast muscle of ducks reared in the RD system than those reared in the FPR system (P < 0.05). Taken together, our results indicated that the RD system improved the carcass traits, intramuscular fat, essential amino acids, and polyunsaturated fatty acids profiles of the ducks. These findings suggest that the RD system is an effective strategy to improve the welfare and meat quality of ducks.