Search

Your search keyword '"Weile, Jan"' showing total 23 results
Start Over Author "Weile, Jan"
23 results on '"Weile, Jan"'

4. Treatment of tularemia in patient with chronic graft-versus-host disease

Author

Weile, Jan, Seibold, Erik, Knabbe, Cornelius, Kaufmann, Martin, and Splettstoesser, Wolf

Subjects
Levofloxacin -- Health aspects, Tularemia -- Care and treatment -- Health aspects, Infection -- Care and treatment -- Health aspects, Health
Abstract

Tularemia is a zoonotic infection caused by the gramnegative bacterium Francisella tularensis. Humans are accidental hosts; infection occurs after contact with infected animals, contaminated water or soil, or invertebrate vectors [...]

Published
2013
Full Text
View/download PDF

19. Cefotaxime Resistant Escherichia coli Collected from a Healthy Volunteer; Characterisation and the Effect of Plasmid Loss.

Author

Kirchner, Miranda, AbuOun, Manal, Mafura, Muriel, Bagnall, Mary, Hunt, Theresa, Thomas, Christopher, Weile, Jan, and Anjum, Muna F.

Subjects
CEFOTAXIME, ESCHERICHIA coli, PLASMIDS, DRUG resistance, POLYMERASE chain reaction, MICROBIAL virulence, ANTIBIOTICS
Abstract

In this study 6 CTX-M positive E. coli isolates collected during a clinical study examining the effect of antibiotic use in a human trial were analysed. The aim of the study was to analyse these isolates and assess the effect of full or partial loss of plasmid genes on bacterial fitness and pathogenicity. A DNA array was utilised to assess resistance and virulence gene carriage. Plasmids were characterised by PCR-based replicon typing and addiction system multiplex PCR. A phenotypic array and insect virulence model were utilised to assess the effect of plasmid-loss in E. coli of a large multi-resistance plasmid. All six E. coli carrying blaCTX-M-14 were detected from a single participant and were identical by pulse field gel electrophoresis and MLST. Plasmid profiling and arrays indicated absence of a large multi-drug resistance (MDR) F-replicon plasmid carrying blaTEM, aadA4, strA, strB, dfrA17/19, sul1, and tetB from one isolate. Although this isolate partially retained the plasmid it showed altered fitness characteristics e.g. inability to respire in presence of antiseptics, similar to a plasmid-cured strain. However, unlike the plasmid-cured or plasmid harbouring strains, the survival rate for Galleria mellonella infected by the former strain was approximately 5-times lower, indicating other possible changes accompanying partial plasmid loss. In conclusion, our results demonstrated that an apparently healthy individual can harbour blaCTX-M-14E. coli strains. In one such strain, isolated from the same individual, partial absence of a large MDR plasmid resulted in altered fitness and virulence characteristics, which may have implications in the ability of this strain to infect and any subsequent treatment. [ABSTRACT FROM AUTHOR]

Published
2013
Full Text
View/download PDF

20. DNA Microarray for Genotyping Antibiotic Resistance Determinants in Acinetobacter baumanniiClinical Isolates

Author

Dally, Simon, Lemuth, Karin, Kaase, Martin, Rupp, Steffen, Knabbe, Cornelius, and Weile, Jan

Abstract

ABSTRACTIn recent decades, Acinetobacter baumanniihas emerged as an organism of great concern due to its ability to accumulate antibiotic resistance. In order to improve the diagnosis of resistance determinants in A. baumanniiin terms of lead time and accuracy, we developed a microarray that can be used to detect 91 target sequences associated with antibiotic resistance within 4 h from bacterial culture to result. The array was validated with 60 multidrug-resistant strains of A. baumanniiin a blinded, prospective study. The results were compared to phenotype results determined by the automated susceptibility testing system VITEK2. Antibiotics considered were piperacillin-tazobactam, ceftazidime, imipenem, meropenem, trimethoprim-sulfamethoxazole, amikacin, gentamicin, tobramycin, ciprofloxacin, and tigecycline. The average positive predictive value, negative predictive value, sensitivity, and specificity were 98, 98, 99, and 94%, respectively. For carbapenemase genes, the array results were compared to singleplex PCR results provided by the German National Reference Center for Gram-Negative Pathogens, and results were in complete concordance. The presented array is able to detect all relevant resistance determinants of A. baumanniiin parallel. The short handling time of 4 h from culture to result helps to provide fast results in order to initiate adequate anti-infective therapy for critically ill patients. Another application would be data acquisition for epidemiologic surveillance.

Published
2013
Full Text
View/download PDF

22. A VIM-1 metallo-beta-lactamase-producing Klebsiella pneumoniae clinical isolate in an acute hospital in Germany.

Author

Weile J, Ohler S, Schönthal S, and Knabbe C

Subjects
Anti-Bacterial Agents pharmacology, Carbapenems pharmacology, Gene Order, Genes, Bacterial, Germany, Hospitals, Humans, Microbial Sensitivity Tests, Plasmids, beta-Lactamases biosynthesis, Klebsiella Infections microbiology, Klebsiella pneumoniae enzymology, Klebsiella pneumoniae isolation & purification, beta-Lactam Resistance
Published
2009
Full Text
View/download PDF

23. First detection of a VIM-1 metallo-beta-lactamase in a carbapenem-resistant Citrobacter freundii clinical isolate in an acute hospital in Germany.

Author

Weile J, Rahmig H, Gfröer S, Schroeppel K, Knabbe C, and Susa M

Subjects
Aged, 80 and over, Cilastatin therapeutic use, Ciprofloxacin therapeutic use, Citrobacter freundii drug effects, Citrobacter freundii genetics, Citrobacter freundii isolation & purification, Enterobacteriaceae Infections drug therapy, Enterobacteriaceae Infections microbiology, Female, Germany, Humans, Imipenem therapeutic use, Polymerase Chain Reaction methods, beta-Lactam Resistance genetics, beta-Lactamases genetics, beta-Lactamases metabolism, Carbapenems pharmacology, Citrobacter freundii enzymology
Abstract

We report the first detection of a carbapenem-resistant Citrobacter freundii clinical strain in Germany. It was isolated from an abscess of a patient with acute necrotic pancreatitis in an acute hospital. PCR and sequencing experiments revealed that the carbapenem resistance was mediated by a VIM-1 metallo-beta-lactamase, located on a plasmid encoded class 1 integron. Carbapenem resistance in Enterobacteriaceae is so far a rare event and 1 of the major therapeutic concerns in the future.

Published
2007
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results