209 results on '"Weihua Zeng"'
Search Results
2. Cost-effective methylome sequencing of cell-free DNA for accurately detecting and locating cancer
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Mary L. Stackpole, Weihua Zeng, Shuo Li, Chun-Chi Liu, Yonggang Zhou, Shanshan He, Angela Yeh, Ziye Wang, Fengzhu Sun, Qingjiao Li, Zuyang Yuan, Asli Yildirim, Pin-Jung Chen, Paul Winograd, Benjamin Tran, Yi-Te Lee, Paul Shize Li, Zorawar Noor, Megumi Yokomizo, Preeti Ahuja, Yazhen Zhu, Hsian-Rong Tseng, James S. Tomlinson, Edward Garon, Samuel French, Clara E. Magyar, Sarah Dry, Clara Lajonchere, Daniel Geschwind, Gina Choi, Sammy Saab, Frank Alber, Wing Hung Wong, Steven M. Dubinett, Denise R. Aberle, Vatche Agopian, Steven-Huy B. Han, Xiaohui Ni, Wenyuan Li, and Xianghong Jasmine Zhou
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Science - Abstract
Abstract Early cancer detection by cell-free DNA faces multiple challenges: low fraction of tumor cell-free DNA, molecular heterogeneity of cancer, and sample sizes that are not sufficient to reflect diverse patient populations. Here, we develop a cancer detection approach to address these challenges. It consists of an assay, cfMethyl-Seq, for cost-effective sequencing of the cell-free DNA methylome (with > 12-fold enrichment over whole genome bisulfite sequencing in CpG islands), and a computational method to extract methylation information and diagnose patients. Applying our approach to 408 colon, liver, lung, and stomach cancer patients and controls, at 97.9% specificity we achieve 80.7% and 74.5% sensitivity in detecting all-stage and early-stage cancer, and 89.1% and 85.0% accuracy for locating tissue-of-origin of all-stage and early-stage cancer, respectively. Our approach cost-effectively retains methylome profiles of cancer abnormalities, allowing us to learn new features and expand to other cancer types as training cohorts grow.
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- 2022
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3. A novel regional-scale human health risk assessment model for soil heavy metal(loid) pollution based on empirical Bayesian kriging
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Liting Wang, Renzhi Liu, Jing Liu, Yushun Qi, Weihua Zeng, and Baoshan Cui
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Heavy metal(loid) ,Soil pollution ,Human health risk assessment ,Empirical Bayesian kriging ,Land use ,Environmental pollution ,TD172-193.5 ,Environmental sciences ,GE1-350 - Abstract
Soil heavy metal(loid)s contamination caused by rapid urbanization and industrialization seriously affects human health and hinders the global sustainable development goals (SDGs). Currently, there is a lack of comprehensive human health risk assessment (HHRA) studies for multiple land use types at the regional scale. We propose a practical risk assessment framework that integrates empirical Bayesian kriging (EBK), pollution level analyses, and modified HHRA modeling. The concentrations of copper industry-related metals (Cu, Ni, Cd, As, and Hg) in 332 topsoil samples from the south bank of the Yangtze River in Tongling were investigated. Obvious enrichment of Cu, Cd, As, and Hg was detected, and the average concentration of Cu was 5.24 times higher than the background values. The distribution of heavy metal(loid) pollution was typically high in the south and east, and low in the north and west. The mean errors of interpolation for Cu, Ni, and Hg were 0.84, 1.29, and 0, respectively, and the root mean square errors of interpolation for Cd and As were 1.29 and 0.86, respectively. Non-carcinogenic risks of soil heavy metal(loid)s were assessed as acceptable throughout the studied area. The hazard index decreased in the order As (0.448) > Ni (0.0729) > Cd (0.0136) > Hg (9.04 ×10−4) > Cu (6.41 ×10−4). Nevertheless, the carcinogenic risks of Ni, Cd, and As in 70–80% of the administrative units (AUs) were between 10−6 to 10−4, considered an unacceptable level. Exposure through the oral ingestion route accounted for 88.0–99.2% of the total three exposure routes. It is worth noting that four AUs were considered to be the priority control units, and Ni and As were identified as the priority control soil heavy metal(loid)s. This case demonstrates the feasibility and scientific validity of the new EBK-HHRA framework, which confirms that EBK can effectively predict the spatial distribution patterns of soil heavy metal(loid)s and that modified HHRA models are conducive to risk integration at the regional scale. The EBK-HHRA approach is generic and provides substantial support for risk source identification and risk management of soil heavy metal(loid)s contamination at the regional scale.
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- 2023
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4. Deep learning-powered 3D segmentation derives factors associated with lymphovascular invasion and prognosis in clinical T1 stage non-small cell lung cancer
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Zhichao Zuo, Xiaohong Fan, Yao Tang, Yang Zhang, Xin Peng, Weihua Zeng, and Ying Zeng
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Artificial intelligence ,Deep learning ,3D segmentation ,Clinical T1 stage non-small cell lung cancer ,Lymphovascular invasion ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Background: Lymphovascular invasion (LVI) is an invasive biologic behavior that affects the treatment and prognosis of patients with early-stage lung cancer. This study aimed to identify LVI diagnostic and prognostic biomarkers using deep learning-powered 3D segmentation with artificial intelligence (AI) technology. Methods: Between January 2016 and October 2021, we enrolled patients with clinical T1 stage non-small cell lung cancer (NSCLC). We used commercially available AI software (Dr. Wise system, Deep-wise Corporation, China) to extract quantitative AI features of pulmonary nodules automatically. Dimensionality reduction was achieved through least absolute shrinkage and selection operator regression; subsequently, the AI score was calculated.Then, the univariate and multivariate analysis was further performed on the AI score and patient baseline parameters. Results: Among 175 enrolled patients, 22 tested positive for LVI at pathology review. Based on the multivariate logistic regression results, we incorporated the AI score, carcinoembryonic antigen, spiculation, and pleural indentation into the nomogram for predicting LVI. The nomogram showed good discrimination (C-index = 0.915 [95% confidence interval: 0.89–0.94]); moreover, calibration of the nomogram revealed good predictive ability (Brier score = 0.072). Kaplan–Meier analysis revealed that relapse-free survival and overall survival were significantly higher among patients with a low-risk AI score and without LVI than those among patients with a high-risk AI score (p = 0.008 and p = 0.002, respectively) and with LVI (p = 0.013 and p = 0.008, respectively). Conclusions: Our findings indicate that a high-risk AI score is a diagnostic biomarker for LVI in patients with clinical T1 stage NSCLC; accordingly, it can serve as a prognostic biomarker for these patients.
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- 2023
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5. The Value of Spectral CT Quantitative Analysis in the Differential Diagnosis of Lung Squamous Cell Carcinoma and Adenocarcinoma
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Shuiting LI, Yucun MO, Huanming ZHANG, and Weihua ZENG
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energy spectrum ct ,lung cancer ,energy spectrum curve ,Geophysics. Cosmic physics ,QC801-809 ,Medicine (General) ,R5-920 - Abstract
Objective: To investigate the value of energy spectrum CT multi-parameter quantitative analysis in the differential diagnosis of lung squamous cell carcinoma and adenocarcinoma. Methods: Seventy-five patients with lung cancer who were confirmed by pathology and underwent energy spectrum CT scanning were collected, including 40 cases of squamous cell carcinoma and 35 cases of adenocarcinoma. We measured and compared the energy spectrum parameters of arterial phase and venous phase between the two groups. Results: The spectrum curve of adenocarcinoma (λ40~65 keV) in arterial and venous phase, effective atomic number, concentration of iodine (water) and standardized iodine concentration were greater than that of squamous carcinoma, the difference was statistically significant, the difference of water (iodine) concentration between the two groups of arterial phase held no statistical significance, the water (iodine) concentration of adenocarcinoma was lower than that of squamous cell carcinomas, the difference was statistically significant. The results of ROC curve analysis showed that the energy spectrum (λ40~65 keV) in the venous phase was of great value in the differentiation of squamous cell carcinoma and adenocarcinoma, with the AUC of 0.839, the sensitivity of 88.6% and the specificity of 72.5%. Conclusion: Adenocarcinoma and squamous cell carcinoma of lung show different energy spectrum CT parameters. It holds certain value to distinguish them, which can provide a new method for preoperative diagnosis.
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- 2022
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6. Survival benefit after neoadjuvant or adjuvant radiotherapy for stage II–III gastroesophageal junction adenocarcinoma: A large population-based cohort study
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Zhichao Zuo, Yafeng Peng, Ying Zeng, Shanyue Lin, Weihua Zeng, Xiao Zhou, Yinjun Zhou, Bo Li, Jie Ma, Mingju Long, Shenghui Cao, and Yang Liu
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neoadjuvant radiotherapy ,adjuvant radiotherapy ,survival ,gastric cancer ,gastroesophageal junction adenocarcinoma (GEJA) ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
ObjectiveThe standard treatment for stage II–III gastroesophageal junction adenocarcinoma (GEJA) remains controversial, and the role of radiotherapy (RT) in stage II–III GEJA is unclear. Herein, we aimed to evaluate the prognosis of different RT sequences and identify potential candidates to undergo neoadjuvant RT (NART) or adjuvant RT (ART).Materials and methodsIn total, we enrolled 3,492 patients with resectable stage II–III GEJA from the Surveillance, Epidemiology, and End Results (SEER) database, subsequently assigned to three categories: T1–2N+, T3–4N−, and T3–4N+. Survival curves were evaluated using the Kaplan–Meier method along with the log-rank test. We compared survival curves for NART, ART, and non-RT in the three categories. To further determine histological types impacting RT-associated survival, we proposed new categories by combining the tumor, node, and metastasis (TNM) stage with Lauren’s classification.ResultsART afforded a significant survival benefit in patients with T1–2N+ and T3–4N+ tumors. In addition, NART conferred a survival advantage in patients with T3–4N+ and T3–4 exhibiting the intestinal type. Notably, ART and NART were both valuable in patients with T3–4N+, although no significant differences between treatment regimens were noted.ConclusionsBoth NART and ART can prolong the survival of patients with stage II–III GEJA. Nevertheless, the selection of NART or ART requires a concrete analysis based on the patient’s condition.
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- 2022
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7. Sensitive detection of tumor mutations from blood and its application to immunotherapy prognosis
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Shuo Li, Zorawar S. Noor, Weihua Zeng, Mary L. Stackpole, Xiaohui Ni, Yonggang Zhou, Zuyang Yuan, Wing Hung Wong, Vatche G. Agopian, Steven M. Dubinett, Frank Alber, Wenyuan Li, Edward B. Garon, and Xianghong Jasmine Zhou
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Science - Abstract
It is possible to call single-nucleotide variant (SNV) in cell-free DNA (cfDNA), but the accuracy of detection is often affected by low tumour cfDNA content. Here, the authors develop a method, cfSNV, and show that it can be used even for medium-coverage whole exome sequencing of cfDNA.
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- 2021
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8. Construction of a human-wildlife spatial interaction index in the Three-River Source Region, China
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Bingran Ma, Yuxi Xie, Tongzuo Zhang, Weihua Zeng, and Yinglan Xue
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Human-wildlife interaction ,Species habitat suitability ,Human impact intensity ,Three-River Source Region ,Ecology ,QH540-549.5 - Abstract
In the context of today’s rapid economic development and increasingly strict ecological protection, interactions between humans and wildlife will intensify. Therefore, we need to identify the spatial distribution of such interactions and to take measures to mitigate possible negative interactions. Using the Three-Rivers Source Region as a case study, we combined species habitat suitability, species feeding habits, species conservation levels and human impact intensity to construct a “human-wildlife spatial interaction index” (HWSII) to evaluate the intensity of human-wildlife spatial interactions. The overall level of HWSII was relatively low in the Three-River Source Region. Higher potential human-wildlife interactions occurred in areas with roads and railways. Areas of relatively high interactions between carnivores and livestock were mainly located in the southern part of the study area, and those with relatively high interactions between herbivores and livestock appeared in the western and eastern parts. Managers in these areas should consider mitigation measures. Although developed for a specific region of China, the ‘human-wildlife spatial interaction index’ could have application in other areas of the world for setting a baseline reference to identify areas with the potential for high levels of human-wildlife interactions and pinpoint places where potential negative interactions can be mitigated.
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- 2021
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9. Realizing Sustainable Development of Yellow River Basin by Horizontal Eco-Compensation Based on Integrated Water Rights (IWRs) Transactions
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Yuxi Xie, Weihua Zeng, Yinglan Xue, and Yue Zhuo
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horizontal eco-compensation ,integrated water rights (IWRs) ,water use rights transaction ,water pollutant discharge rights transaction ,Yellow River basin ,Hydraulic engineering ,TC1-978 ,Water supply for domestic and industrial purposes ,TD201-500 - Abstract
Horizontal/interregional eco-compensation is an important policy to promote regional ecological and environmental cooperation and realize sustainable development for river basins. To solve these problems, a horizontal compensation framework based on integrated water rights (IWRs) transaction has been established. Taking the Yellow River basin as an example, the IWRs scheme realizes sustainable development, to a certain extent, improving the current issues of shortages, the low utilization efficiency of water resources, and weak water environmental carrying capacity. Evidence for this improvement includes: (1) an increased revenue of USD 244.71; (2) the water use efficiency in the middle and upper streams (Qinghai, Gansu, Ningxia, Neimenggu, Shanxi) and the overall river basin being obviously improved (the maximum improvement value is 0.1273 m3/USD); (3) the overload situation of water resource carrying status being relieved in some subareas (Ningxia, Neimenggu, Henan, Shandong) and improved in the whole basin (from 0.6366 to 0.6124); (3) the water environmental carrying rate (COD and NH4+-N) of the middle and upper streams (Qinghai, Gansu, Ningxia, Neimenggu, Shanxi) and the whole river basin obviously decreasing (with maximum decreasing values of 0.43 and 1.14, respectively).
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- 2022
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10. Single-nucleus RNA-seq identifies divergent populations of FSHD2 myotube nuclei.
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Shan Jiang, Katherine Williams, Xiangduo Kong, Weihua Zeng, Nam Viet Nguyen, Xinyi Ma, Rabi Tawil, Kyoko Yokomori, and Ali Mortazavi
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Genetics ,QH426-470 - Abstract
FSHD is characterized by the misexpression of DUX4 in skeletal muscle. Although DUX4 upregulation is thought to be the pathogenic cause of FSHD, DUX4 is lowly expressed in patient samples, and analysis of the consequences of DUX4 expression has largely relied on artificial overexpression. To better understand the native expression profile of DUX4 and its targets, we performed bulk RNA-seq on a 6-day differentiation time-course in primary FSHD2 patient myoblasts. We identify a set of 54 genes upregulated in FSHD2 cells, termed FSHD-induced genes. Using single-cell and single-nucleus RNA-seq on myoblasts and differentiated myotubes, respectively, we captured, for the first time, DUX4 expressed at the single-nucleus level in a native state. We identified two populations of FSHD myotube nuclei based on low or high enrichment of DUX4 and FSHD-induced genes ("FSHD-Lo" and "FSHD Hi", respectively). FSHD-Hi myotube nuclei coexpress multiple DUX4 target genes including DUXA, LEUTX and ZSCAN4, and also upregulate cell cycle-related genes with significant enrichment of E2F target genes and p53 signaling activation. We found more FSHD-Hi nuclei than DUX4-positive nuclei, and confirmed with in situ RNA/protein detection that DUX4 transcribed in only one or two nuclei is sufficient for DUX4 protein to activate target genes across multiple nuclei within the same myotube. DUXA (the DUX4 paralog) is more widely expressed than DUX4, and depletion of DUXA suppressed the expression of LEUTX and ZSCAN4 in late, but not early, differentiation. The results suggest that the DUXA can take over the role of DUX4 to maintain target gene expression. These results provide a possible explanation as to why it is easier to detect DUX4 target genes than DUX4 itself in patient cells and raise the possibility of a self-sustaining network of gene dysregulation triggered by the limited DUX4 expression.
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- 2020
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11. The effect of Nipped-B-like (Nipbl) haploinsufficiency on genome-wide cohesin binding and target gene expression: modeling Cornelia de Lange syndrome
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Daniel A. Newkirk, Yen-Yun Chen, Richard Chien, Weihua Zeng, Jacob Biesinger, Ebony Flowers, Shimako Kawauchi, Rosaysela Santos, Anne L. Calof, Arthur D. Lander, Xiaohui Xie, and Kyoko Yokomori
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CdLS ,Cohesin ,Nipbl ,Haploinsufficiency ,Chromatin immunoprecipitation (ChIP) ,Gene regulation ,Medicine ,Genetics ,QH426-470 - Abstract
Abstract Background Cornelia de Lange syndrome (CdLS) is a multisystem developmental disorder frequently associated with heterozygous loss-of-function mutations of Nipped-B-like (NIPBL), the human homolog of Drosophila Nipped-B. NIPBL loads cohesin onto chromatin. Cohesin mediates sister chromatid cohesion important for mitosis but is also increasingly recognized as a regulator of gene expression. In CdLS patient cells and animal models, expression changes of multiple genes with little or no sister chromatid cohesion defect suggests that disruption of gene regulation underlies this disorder. However, the effect of NIPBL haploinsufficiency on cohesin binding, and how this relates to the clinical presentation of CdLS, has not been fully investigated. Nipbl haploinsufficiency causes CdLS-like phenotype in mice. We examined genome-wide cohesin binding and its relationship to gene expression using mouse embryonic fibroblasts (MEFs) from Nipbl+/− mice that recapitulate the CdLS phenotype. Results We found a global decrease in cohesin binding, including at CCCTC-binding factor (CTCF) binding sites and repeat regions. Cohesin-bound genes were found to be enriched for histone H3 lysine 4 trimethylation (H3K4me3) at their promoters; were disproportionately downregulated in Nipbl mutant MEFs; and displayed evidence of reduced promoter-enhancer interaction. The results suggest that gene activation is the primary cohesin function sensitive to Nipbl reduction. Over 50% of significantly dysregulated transcripts in mutant MEFs come from cohesin target genes, including genes involved in adipogenesis that have been implicated in contributing to the CdLS phenotype. Conclusions Decreased cohesin binding at the gene regions is directly linked to disease-specific expression changes. Taken together, our Nipbl haploinsufficiency model allows us to analyze the dosage effect of cohesin loading on CdLS development.
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- 2017
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12. Simulation of the Urban Jobs–Housing Location Selection and Spatial Relationship Using a Multi-Agent Approach
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Huihui Wang, Weihua Zeng, and Ruoxin Cao
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jobs–housing relationship ,agent-based modeling ,location decision-making ,Beijing ,Geography (General) ,G1-922 - Abstract
The jobs–housing balance concerns the spatial relationship between the number of jobs and housing units within a given geographical area. Due to the separation of jobs and housing, spatial dislocations have occurred in large cities, which have resulted in a significant increase in commuting distance and time. These changes have ultimately led to an increase in pressure on urban traffic, and the formation of tidal traffic. In this study we introduce a multi-agent approach to examine the jobs–housing relationship under the maximum location utility of agents. The jobs/housing ratio measures the balance of the of jobs–housing relationship, as well as comparing and analyzing jobs–housing separation in Beijing by district, county, and street scales. An agent-based model was proposed to simulate spatial location selection behavior of agents by considering environmental and economical influences on residential decisions of individuals. Results show that the jobs–housing relationship imbalance in Beijing has been mainly aggravated due to rapid population growth in the 6th Ring Road. An imbalance in the jobs–housing relationship has arisen due to a mismatch with the number of households available compared to the number of jobs; the surrounding urban areas cannot provide the required volume of housing to accommodate the increase in workers. Six sets of experiments were established to examine resident agents and enterprise agents. Differences in resident agents’ income level had a greater impact on residential location decision-making, and housing price was the primary factor affecting the decision of residents to choose their residential location. The spatial distribution of jobs and housing in Beijing under the maximization of micro-agent location utility was obtained in this study. Results indicated that the imbalance in the jobs¬-housing relationship in central Beijing has improved and, compared with the initial distributions, the number of jobs–housing balance areas in Beijing has increased.
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- 2021
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13. Revealing Spatial-Temporal Characteristics and Patterns of Urban Travel: A Large-Scale Analysis and Visualization Study with Taxi GPS Data
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Huihui Wang, Hong Huang, Xiaoyong Ni, and Weihua Zeng
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human travel ,travel pattern ,OD flow ,the chord diagram plot ,taxi data ,Geography (General) ,G1-922 - Abstract
Mobility and spatial interaction data have become increasingly available due to the widespread adoption of location-aware technologies. Examples of mobile data include human daily activities, vehicle trajectories, and animal movements. In this study we focus on a special type of mobility data, i.e., origin–destination (OD) pairs, and propose a new adapted chord diagram plot to reveal the urban human travel spatial-temporal characteristics and patterns of a seven-day taxi trajectory data set collected in Beijing; this large scale data set includes approximately 88.5 million trips of anonymous customers. The spatial distribution patterns of the pick-up points (PUPs) and the drop-off points (DOPs) on weekdays and weekends are analyzed first. The maximum of the morning and the evening peaks are at 8:00–10:00 and 17:00–19:00. The morning peaks of taxis are delayed by 0.5–1 h compared with the commuting morning peaks. Second, travel demand, intensity, time, and distance on weekdays and weekends are analyzed to explore human mobility. The travel demand and high-intensity travel of residents in Beijing is mainly concentrated within the 6th Ring Road. The residents who travel long distances (>10 km) and for a long time (>60 min) mainly from outside the 6th Ring Road and the surrounding new towns of Beijing. The circular structure of the travel distance distribution also confirms the single-center urban structure of Beijing. Finally, a new adapted chord diagram plot is proposed to achieve the spatial-temporal scale visualization of taxi trajectory origin–destination (OD) flows. The method can characterize the volume, direction, and properties of OD flows in multiple spatial-temporal scales; it is implemented using a circular visualization package in R (circlize). Through the visualization experiment of taxi GPS trajectory data in Beijing, the results show that the proposed visualization technology is able to characterize the spatial-temporal patterns of trajectory OD flows in multiple spatial-temporal scales. These results are expected to enhance current urban mobility research and suggest some interesting avenues for future research.
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- 2019
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14. An Energy-Economy-Environment Model for Simulating the Impacts of Socioeconomic Development on Energy and Environment
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Wenyi Wang, Weihua Zeng, and Bo Yao
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Technology ,Medicine ,Science - Abstract
Many rapidly developing regions have begun to draw the attention of the world. Meanwhile, the energy and environmental issues associated with rapid economic growth have aroused widespread critical concern. Therefore, studying energy, economic, and environmental systems is of great importance. This study establishes a system dynamic model that covers multiple aspects of those systems, such as energy, economy, population, water pollution, air pollution, solid waste, and technology. The model designed here attempts to determine the impacts of socioeconomic development on the energy and environment of Tongzhou District in three scenarios: under current, planning, and sustainable conditions. The results reveal that energy shortages and water pollutions are very serious and are the key issues constraining future social and economic development. Solid waste emissions increase with population growth. The prediction results provide valuable insights into social advancement.
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- 2014
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15. Specific loss of histone H3 lysine 9 trimethylation and HP1gamma/cohesin binding at D4Z4 repeats is associated with facioscapulohumeral dystrophy (FSHD).
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Weihua Zeng, Jessica C de Greef, Yen-Yun Chen, Richard Chien, Xiangduo Kong, Heather C Gregson, Sara T Winokur, April Pyle, Keith D Robertson, John A Schmiesing, Virginia E Kimonis, Judit Balog, Rune R Frants, Alexander R Ball, Leslie F Lock, Peter J Donovan, Silvère M van der Maarel, and Kyoko Yokomori
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Genetics ,QH426-470 - Abstract
Facioscapulohumeral dystrophy (FSHD) is an autosomal dominant muscular dystrophy in which no mutation of pathogenic gene(s) has been identified. Instead, the disease is, in most cases, genetically linked to a contraction in the number of 3.3 kb D4Z4 repeats on chromosome 4q. How contraction of the 4qter D4Z4 repeats causes muscular dystrophy is not understood. In addition, a smaller group of FSHD cases are not associated with D4Z4 repeat contraction (termed "phenotypic" FSHD), and their etiology remains undefined. We carried out chromatin immunoprecipitation analysis using D4Z4-specific PCR primers to examine the D4Z4 chromatin structure in normal and patient cells as well as in small interfering RNA (siRNA)-treated cells. We found that SUV39H1-mediated H3K9 trimethylation at D4Z4 seen in normal cells is lost in FSHD. Furthermore, the loss of this histone modification occurs not only at the contracted 4q D4Z4 allele, but also at the genetically intact D4Z4 alleles on both chromosomes 4q and 10q, providing the first evidence that the genetic change (contraction) of one 4qD4Z4 allele spreads its effect to other genomic regions. Importantly, this epigenetic change was also observed in the phenotypic FSHD cases with no D4Z4 contraction, but not in other types of muscular dystrophies tested. We found that HP1gamma and cohesin are co-recruited to D4Z4 in an H3K9me3-dependent and cell type-specific manner, which is disrupted in FSHD. The results indicate that cohesin plays an active role in HP1 recruitment and is involved in cell type-specific D4Z4 chromatin regulation. Taken together, we identified the loss of both histone H3K9 trimethylation and HP1gamma/cohesin binding at D4Z4 to be a faithful marker for the FSHD phenotype. Based on these results, we propose a new model in which the epigenetic change initiated at 4q D4Z4 spreads its effect to other genomic regions, which compromises muscle-specific gene regulation leading to FSHD pathogenesis.
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- 2009
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16. Construction of transformer substation fault knowledge graph based on a depth learning algorithm.
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Deliang Zhu, Weihua Zeng, and Jianming Su
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- 2023
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17. A low-power and low cost smart streetlight system based on Internet of Things technology.
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Jingjing Zhang, Weihua Zeng, Shengli Hou, Yuqi Chen 0014, Linyan Guo, and Yanxing Li
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- 2022
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18. Development of a Multi-Channel Array Resistivity Method Instrument System.
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Weihua Zeng, Jingjing Zhang, Handong Tan, and Shengli Hou
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- 2022
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19. Normalized difference vegetation index prediction based on the delta downscaling method and back-propagation artificial neural network under climate change in the Sanjiangyuan region, China.
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Bingran Ma, Weihua Zeng, Guanzheng Hu, Ruoxin Cao, Dan Cui, and Tongzuo Zhang
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- 2022
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20. Design of an unattended ore grading measurement system in a uranium mine
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Cong Chen, Weihua Zeng, Shengli Hou, and Yike Ding
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Nuclear and High Energy Physics ,Radiation ,Nuclear Energy and Engineering ,General Materials Science ,Safety, Risk, Reliability and Quality - Abstract
The unattended ore grading radioactivity measurement system is an important part of the digital uranium mine. However, with the promotion of the digitization of mines, the radioactivity measurement system for the truck checkpoint used to measure the grade of ore for hydrometallurgical process still maintains the backward processing method of offline measurement and manual recording. To improve the situation, an unattended ore grading measurement system using gamma detectors is developed. Through unattended identification, weighting and radioactivity measurement, the values of the grade of ore and the uranium content are calculated and uploaded automatically. The system has been put into practical use at a uranium mine in southern China in September 2021. Through the field use, the system can realize automatic measurement, reduce labor costs, and establish a production database, which lays the foundation for further application in the construction of digital mines on a large scale.
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- 2022
21. MiR-33a functions as a tumor suppressor in triple-negative breast cancer by targeting EZH2
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Weihua, Zeng, Guorong, Zou, Xiaolong, Cao, and Weizhan, Li
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- 2020
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22. The ENCODE4 long-read RNA-seq collection reveals distinct classes of transcript structure diversity
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Fairlie Reese, Brian Williams, Gabriela Balderrama-Gutierrez, Dana Wyman, Muhammed Hasan Çelik, Elisabeth Rebboah, Narges Rezaie, Diane Trout, Milad Razavi-Mohseni, Yunzhe Jiang, Beatrice Borsari, Samuel Morabito, Heidi Yahan Liang, Cassandra J. McGill, Sorena Rahmanian, Jasmine Sakr, Shan Jiang, Weihua Zeng, Klebea Carvalho, Annika K. Weimer, Louise A. Dionne, Ariel McShane, Karan Bedi, Shaimae I. Elhajjajy, Sean Upchurch, Jennifer Jou, Ingrid Youngworth, Idan Gabdank, Paul Sud, Otto Jolanki, J. Seth Strattan, Meenakshi S. Kagda, Michael P. Snyder, Ben C. Hitz, Jill E. Moore, Zhiping Weng, David Bennett, Laura Reinholdt, Mats Ljungman, Michael A. Beer, Mark B. Gerstein, Lior Pachter, Roderic Guigó, Barbara J. Wold, and Ali Mortazavi
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Article - Abstract
The majority of mammalian genes encode multiple transcript isoforms that result from differential promoter use, changes in exonic splicing, and alternative 3’ end choice. Detecting and quantifying transcript isoforms across tissues, cell types, and species has been extremely challenging because transcripts are much longer than the short reads normally used for RNA-seq. By contrast, long-read RNA-seq (LR-RNA-seq) gives the complete structure of most transcripts. We sequenced 264 LR-RNA-seq PacBio libraries totaling over 1 billion circular consensus reads (CCS) for 81 unique human and mouse samples. We detect at least one full-length transcript from 87.7% of annotated human protein coding genes and a total of 200,000 full-length transcripts, 40% of which have novel exon junction chains.To capture and compute on the three sources of transcript structure diversity, we introduce a gene and transcript annotation framework that uses triplets representing the transcript start site, exon junction chain, and transcript end site of each transcript. Using triplets in a simplex representation demonstrates how promoter selection, splice pattern, and 3’ processing are deployed across human tissues, with nearly half of multitranscript protein coding genes showing a clear bias toward one of the three diversity mechanisms. Evaluated across samples, the predominantly expressed transcript changes for 74% of protein coding genes. In evolution, the human and mouse transcriptomes are globally similar in types of transcript structure diversity, yet among individual orthologous gene pairs, more than half (57.8%) show substantial differences in mechanism of diversification in matching tissues. This initial large-scale survey of human and mouse long-read transcriptomes provides a foundation for further analyses of alternative transcript usage, and is complemented by short-read and microRNA data on the same samples and by epigenome data elsewhere in the ENCODE4 collection.
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- 2023
23. Supplementary Figure from cfTrack: A Method of Exome-Wide Mutation Analysis of Cell-free DNA to Simultaneously Monitor the Full Spectrum of Cancer Treatment Outcomes Including MRD, Recurrence, and Evolution
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Xianghong Jasmine Zhou, Wenyuan Li, Steven M. Dubinett, Edward B. Garon, Sanaz Memarzadeh, Adam Neal, Zuyang Yuan, Zorawar S. Noor, Mary L. Stackpole, Yonggang Zhou, Xiaohui Ni, Weihua Zeng, and Shuo Li
- Abstract
Supplementary Figure from cfTrack: A Method of Exome-Wide Mutation Analysis of Cell-free DNA to Simultaneously Monitor the Full Spectrum of Cancer Treatment Outcomes Including MRD, Recurrence, and Evolution
- Published
- 2023
24. Data from cfTrack: A Method of Exome-Wide Mutation Analysis of Cell-free DNA to Simultaneously Monitor the Full Spectrum of Cancer Treatment Outcomes Including MRD, Recurrence, and Evolution
- Author
-
Xianghong Jasmine Zhou, Wenyuan Li, Steven M. Dubinett, Edward B. Garon, Sanaz Memarzadeh, Adam Neal, Zuyang Yuan, Zorawar S. Noor, Mary L. Stackpole, Yonggang Zhou, Xiaohui Ni, Weihua Zeng, and Shuo Li
- Abstract
Purpose:Cell-free DNA (cfDNA) offers a noninvasive approach to monitor cancer. Here we develop a method using whole-exome sequencing (WES) of cfDNA for simultaneously monitoring the full spectrum of cancer treatment outcomes, including minimal residual disease (MRD), recurrence, evolution, and second primary cancers.Experimental Design:Three simulation datasets were generated from 26 patients with cancer to benchmark the detection performance of MRD/recurrence and second primary cancers. For further validation, cfDNA samples (n = 76) from patients with cancer (n = 35) with six different cancer types were used for performance validation during various treatments.Results:We present a cfDNA-based cancer monitoring method, named cfTrack. Taking advantage of the broad genome coverage of WES data, cfTrack can sensitively detect MRD and cancer recurrence by integrating signals across known clonal tumor mutations of a patient. In addition, cfTrack detects tumor evolution and second primary cancers by de novo identifying emerging tumor mutations. A series of machine learning and statistical denoising techniques are applied to enhance the detection power. On the simulation data, cfTrack achieved an average AUC of 99% on the validation dataset and 100% on the independent dataset in detecting recurrence in samples with tumor fractions ≥0.05%. In addition, cfTrack yielded an average AUC of 88% in detecting second primary cancers in samples with tumor fractions ≥0.2%. On real data, cfTrack accurately monitors tumor evolution during treatment, which cannot be accomplished by previous methods.Conclusions:Our results demonstrated that cfTrack can sensitively and specifically monitor the full spectrum of cancer treatment outcomes using exome-wide mutation analysis of cfDNA.
- Published
- 2023
25. Supplementary Table from cfTrack: A Method of Exome-Wide Mutation Analysis of Cell-free DNA to Simultaneously Monitor the Full Spectrum of Cancer Treatment Outcomes Including MRD, Recurrence, and Evolution
- Author
-
Xianghong Jasmine Zhou, Wenyuan Li, Steven M. Dubinett, Edward B. Garon, Sanaz Memarzadeh, Adam Neal, Zuyang Yuan, Zorawar S. Noor, Mary L. Stackpole, Yonggang Zhou, Xiaohui Ni, Weihua Zeng, and Shuo Li
- Abstract
Supplementary Table from cfTrack: A Method of Exome-Wide Mutation Analysis of Cell-free DNA to Simultaneously Monitor the Full Spectrum of Cancer Treatment Outcomes Including MRD, Recurrence, and Evolution
- Published
- 2023
26. Supplementary Figures 1-5 from Inhibitors of the Proteasome Suppress Homologous DNA Recombination in Mammalian Cells
- Author
-
Shunichi Takeda, Simon J. Boulton, Helfrid Hochegger, Guang Yu Zhao, Alan Lehmann, Atsuko Niimi, Hiroshi Kimura, Kyoko Yokomori, Weihua Zeng, Louise J. Barber, Eiichiro Sonoda, and Yasuhiro Murakawa
- Abstract
Supplementary Figures 1-5 from Inhibitors of the Proteasome Suppress Homologous DNA Recombination in Mammalian Cells
- Published
- 2023
27. Data from Inhibitors of the Proteasome Suppress Homologous DNA Recombination in Mammalian Cells
- Author
-
Shunichi Takeda, Simon J. Boulton, Helfrid Hochegger, Guang Yu Zhao, Alan Lehmann, Atsuko Niimi, Hiroshi Kimura, Kyoko Yokomori, Weihua Zeng, Louise J. Barber, Eiichiro Sonoda, and Yasuhiro Murakawa
- Abstract
Proteasome inhibitors are novel antitumor agents against multiple myeloma and other malignancies. Despite the increasing clinical application, the molecular basis of their antitumor effect has been poorly understood due to the involvement of the ubiquitin-proteasome pathway in multiple cellular metabolisms. Here, we show that treatment of cells with proteasome inhibitors has no significant effect on nonhomologous end joining but suppresses homologous recombination (HR), which plays a key role in DNA double-strand break (DSB) repair. In this study, we treat human cells with proteasome inhibitors and show that the inhibition of the proteasome reduces the efficiency of HR-dependent repair of an artificial HR substrate. We further show that inhibition of the proteasome interferes with the activation of Rad51, a key factor for HR, although it does not affect the activation of ATM, γH2AX, or Mre11. These data show that the proteasome-mediated destruction is required for the promotion of HR at an early step. We suggest that the defect in HR-mediated DNA repair caused by proteasome inhibitors contributes to antitumor effect, as HR plays an essential role in cellular proliferation. Moreover, because HR plays key roles in the repair of DSBs caused by chemotherapeutic agents such as cisplatin and by radiotherapy, proteasome inhibitors may enhance the efficacy of these treatments through the suppression of HR-mediated DNA repair pathways. [Cancer Res 2007;67(18):8536–43]
- Published
- 2023
28. Spatiotemporal spillover effect and efficiency of carbon emissions from land use in China
- Author
-
Huihui Wang, Yingyan He, Wanyang Shi, Weihua Zeng, and Yifeng He
- Subjects
Economics and Econometrics ,Geography, Planning and Development ,Management, Monitoring, Policy and Law - Published
- 2023
29. cfTrack: A Method of Exome-Wide Mutation Analysis of Cell-free DNA to Simultaneously Monitor the Full Spectrum of Cancer Treatment Outcomes Including MRD, Recurrence, and Evolution
- Author
-
Shuo Li, Weihua Zeng, Xiaohui Ni, Yonggang Zhou, Mary L. Stackpole, Zorawar S. Noor, Zuyang Yuan, Adam Neal, Sanaz Memarzadeh, Edward B. Garon, Steven M. Dubinett, Wenyuan Li, and Xianghong Jasmine Zhou
- Subjects
Cancer Research ,Neoplasm, Residual ,Treatment Outcome ,Oncology ,Mutation ,Exome Sequencing ,Biomarkers, Tumor ,Humans ,Exome ,Neoplasms, Second Primary ,Neoplasm Recurrence, Local ,Cell-Free Nucleic Acids ,Article - Abstract
Purpose:Cell-free DNA (cfDNA) offers a noninvasive approach to monitor cancer. Here we develop a method using whole-exome sequencing (WES) of cfDNA for simultaneously monitoring the full spectrum of cancer treatment outcomes, including minimal residual disease (MRD), recurrence, evolution, and second primary cancers.Experimental Design:Three simulation datasets were generated from 26 patients with cancer to benchmark the detection performance of MRD/recurrence and second primary cancers. For further validation, cfDNA samples (n = 76) from patients with cancer (n = 35) with six different cancer types were used for performance validation during various treatments.Results:We present a cfDNA-based cancer monitoring method, named cfTrack. Taking advantage of the broad genome coverage of WES data, cfTrack can sensitively detect MRD and cancer recurrence by integrating signals across known clonal tumor mutations of a patient. In addition, cfTrack detects tumor evolution and second primary cancers by de novo identifying emerging tumor mutations. A series of machine learning and statistical denoising techniques are applied to enhance the detection power. On the simulation data, cfTrack achieved an average AUC of 99% on the validation dataset and 100% on the independent dataset in detecting recurrence in samples with tumor fractions ≥0.05%. In addition, cfTrack yielded an average AUC of 88% in detecting second primary cancers in samples with tumor fractions ≥0.2%. On real data, cfTrack accurately monitors tumor evolution during treatment, which cannot be accomplished by previous methods.Conclusions:Our results demonstrated that cfTrack can sensitively and specifically monitor the full spectrum of cancer treatment outcomes using exome-wide mutation analysis of cfDNA.
- Published
- 2022
30. Overloading risk assessment of water environment-water resources carrying capacity based on a novel Bayesian methodology
- Author
-
Liting Wang, Weihua Zeng, Ruoxin Cao, Yue Zhuo, Jie Fu, and Jianping Wang
- Subjects
Water Science and Technology - Published
- 2023
31. Comprehensive tissue deconvolution of cell-free DNA by deep learning for disease diagnosis and monitoring.
- Author
-
Shuo Li, Weihua Zeng, Xiaohui Ni, Qiao Liu, Wenyuan Li, Stackpole, Mary L., Yonggang Zhou, Gower, Arjan, Krysan, Kostyantyn, Ahuja, Preeti, Lu, David S., Raman, Steven S., Hsu, William, Aberle, Denise R., Magyar, Clara E., French, Samuel W., Hane, Steven-Huy B., Garon, Edward B., Agopian, Vatche G., and Wing Hung Wong
- Subjects
- *
CELL-free DNA , *DEEP learning , *DIAGNOSIS , *TISSUES , *CELL death , *CANCER education - Abstract
Plasma cell-free DNA (cfDNA) is a noninvasive biomarker for cell death of all organs. Deciphering the tissue origin of cfDNA can reveal abnormal cell death because of dis- eases, which has great clinical potential in disease detection and monitoring. Despite the great promise, the sensitive and accurate quantification of tissue-derived cfDNA remains challenging to existing methods due to the limited characterization of tissue methylation and the reliance on unsupervised methods. To fully exploit the clinical potential of tissue-derived cfDNA, here we present one of the largest comprehensive and high- resolution methylation atlas based on 521 noncancer tissue samples spanning 29 major types of human tissues. We systematically identified fragment-level tissue-specific methylation patterns and extensively validated them in orthogonal datasets. Based on the rich tissue methylation atlas, we develop the first supervised tissue deconvolution approach, a deep-learning-powered model, cfSort, for sensitive and accurate tissue decon- volution in cfDNA. On the benchmarking data, cfSort showed superior sensitivity and accuracy compared to the existing methods. We further demonstrated the clinical util- ities of cfSort with two potential applications: aiding disease diagnosis and monitoring treatment side effects. The tissue-derived cfDNA fraction estimated from cfSort reflected the clinical outcomes of the patients. In summary, the tissue methylation atlas and cfSort enhanced the performance of tissue deconvolution in cfDNA, thus facilitating cfDNA-based disease detection and longitudinal treatment monitoring. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
32. Research on the Construction Safety Accident Analysis Model of Deep Excavation Project Caused by Rainstorm
- Author
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Junfang Rao, Wei Chen, Weihua Zeng, Daoyuan Guo, and Yishuai Tian
- Published
- 2022
33. Construction of transformer substation fault knowledge graph based on a depth learning algorithm
- Author
-
Deliang Zhu, Weihua Zeng, and Jianming Su
- Subjects
Modeling and Simulation ,General Mathematics ,General Engineering ,Computer Science Applications - Abstract
A knowledge graph is a visual method that can display the information contained in the knowledge points, core structure, and comprehensive knowledge structure technology. In recent years, with the innovation of science and technology, the business field became keen on knowledge graphs and the graphical display method. However, the application of knowledge graphs in the business field is mainly limited to search engines, question, and answer systems because of the technical difficulties of knowledge extraction and knowledge graph drawing of unstructured text, especially the knowledge extraction of amorphous culture. It can provide knowledgeable service to users by analyzing the knowledge entity contained in encyclopedia knowledge or knowledge base. This paper will focus on the critical link of knowledge extraction of the knowledge graph, adopt a depth learning algorithm to solve this urgent problem and combine with the application of knowledge graph in substation fault to analyze the construction process of substation fault knowledge map based on AI.
- Published
- 2022
34. Research on the Contribution of Structure Adjustment on Carbon Dioxide Emissions Reduction based on LMDI Method.
- Author
-
Leya Wu and Weihua Zeng
- Published
- 2013
- Full Text
- View/download PDF
35. Research on ANN-based assessment model of social renewability of city water resources.
- Author
-
Weihua Zeng, Jingjing Shi, and Jie Zhu
- Published
- 2011
- Full Text
- View/download PDF
36. Research on ANN-based model of joint collocation of water quantity and quality.
- Author
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Weihua Zeng, Bo Yao, Tao Wang, and Hengchen Liu
- Published
- 2010
- Full Text
- View/download PDF
37. High‐gain <scp>phase‐gradient metasurface</scp> lens antenna for 5.5–6.5 <scp>GHz</scp> with back cavity
- Author
-
Xiangyu Lin, Linyan Guo, Yajun Zhou, Lianfeng Chen, and Weihua Zeng
- Subjects
Electrical and Electronic Engineering ,Computer Graphics and Computer-Aided Design ,Computer Science Applications - Published
- 2022
38. A 1‐bit reconfigurable metasurface antenna for ground penetrating radar application
- Author
-
Ziye Wang, Weihua Zeng, Xiao Zhao, Linyan Guo, and Yan Liu
- Subjects
Electrical and Electronic Engineering ,Computer Graphics and Computer-Aided Design ,Computer Science Applications - Published
- 2022
39. Simulation of urban transport carbon dioxide emission reduction environment economic policy in China: An integrated approach using agent-based modelling and system dynamics
- Author
-
Huihui Wang, Wanyang Shi, Wanlin He, Hanyu Xue, and Weihua Zeng
- Subjects
Renewable Energy, Sustainability and the Environment ,Strategy and Management ,Building and Construction ,Industrial and Manufacturing Engineering ,General Environmental Science - Published
- 2023
40. Analysis and Designing of System on Sharing EST Information.
- Author
-
Weihua Zeng, Han Shi, Rongchang Chen, and Yongjun Zhang
- Published
- 2003
41. Development of a portable TDCR system at NIM, China
- Author
-
Qianqian Zhou, Haoran Liu, Zhijie Yang, Weihua Zeng, and Juncheng Liang
- Subjects
Radioisotopes ,China ,Radiation ,Scintillation Counting - Abstract
In order to meet the demand of on-site measurement for radionuclides, a portable liquid scintillation TDCR system was developed at National Institute of Metrology (NIM), China. The system consists small size TDCR counter for the measurement of liquid scintillation sources, and digital electronics for pulse signal processing. The optical chamber adopts Teflon material with high diffuse reflection efficiency. Two independent signal processing solutions were used here for TDCR counting. One employed the on-line TDCR solution based on FPGA counting module named TDCR-DMCA, and the other adopted the off-line TDCR solution based on a stand-alone desktop digitizer of CAEN. Two solutions are applied to perform coincidence, dead-time and counting operations follow by MAC3 logic. The performance of the TDCR counting system was tested in benchmark comparison with the traditional custom-built TDCR counting system at NIM through activity measurements of
- Published
- 2022
42. Impacts of Climate Change on Natural Hazards Caused by Extreme Climate Events in Chinese National Parks from 1961 to 2020
- Author
-
Congyu Hou, Bingran Ma, Wei Ouyang, Jingyi Hu, Li Ling, Weihua Zeng, and Zhifeng Yang
- Subjects
History ,Polymers and Plastics ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2022
43. Indoor and outdoor 222Rn and 220Rn and their progeny levels surrounding Bayan Obo mine, China
- Author
-
Miao Hu, Cong Yu, Nanping Wang, Binlin Jia, Weihua Zeng, and Zhijie Yang
- Subjects
Physics ,Nuclear and High Energy Physics ,Earth science ,010501 environmental sciences ,Condensed Matter Physics ,01 natural sciences ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,0302 clinical medicine ,Nuclear Energy and Engineering ,Safety, Risk, Reliability and Quality ,China ,Waste Management and Disposal ,Instrumentation ,0105 earth and related environmental sciences - Abstract
More than half of the total natural ionizing radiation dose received by the human population is caused by radon and thoron (Rn and Tn) and their progeny. To estimate the level of radiation due to radon and thoron and their progeny, an investigation was conducted in a residential area near the world’s largest open-pit mine of Bayan Obo in Inner Mongolia, China. The concentration of Rn, Tn, and their decay products in air and soil were studied by using AlphaGUARD, RAD7, and ERS-RDM-2S for a discrete period of time in three different locations. The average indoor concentration of radon and thoron was 62.6 ± 44.6 Bq/m3 and 108.3 ± 94.5 Bq/m3 respectively, and the outdoor concentration was 12.9 ± 6.3 Bq/m3 and 55.8 ± 18.5 Bq/m3, respectively. Relatively high concentrations were recorded in the area near to the mine, with a significant increasing trend observed in indoor thoron concentration. A prominent hotspot in thoron concentration was found in a single-story house with values 747 ± 150 Bq/m3. The equilibrium equivalent thoron concentration (EECTn) varies from 0.48 Bq/m3 to 2.36 Bq/m3 with an arithmetic mean of 1.37 ± 0.64 Bq/m3, and comparatively higher than EECRn. Concluding that the mining activity at Bayan Obo mine is significantly increasing the level of indoor thoron and its progeny in surroundings. It is suggested to further systematically investigate the indoor Rn and Tn progeny concentrations in the residential dwellings of the Bayan Obo mining area, and 232Th content of the building materials, to provide a basis for calculating the radiation dose.
- Published
- 2020
44. Impact of 17β-Estradiol on Natural Water’s Heterotrophic Nitrifying Bacteria
- Author
-
Ziyi Dong, Changhao Xiao, Weihua Zeng, and Jinbo Zhao
- Subjects
010504 meteorology & atmospheric sciences ,biology ,Chemistry ,Natural water ,Heterotroph ,010501 environmental sciences ,biology.organism_classification ,Positive correlation ,01 natural sciences ,Nitrifying bacteria ,Environmental chemistry ,Food science ,Nitrogen source ,Bacteria ,Volume concentration ,0105 earth and related environmental sciences ,General Environmental Science - Abstract
In this research, bottom water samples were collected from nature water. After cultivating and selecting, bacteria which could use (NH4)2SO4 as the only nitrogen source had been selected. The bacteria were cultivated in BM cultures with 0, 0.1, 1, 10, 100 ng/L 17β-estradiol (E2), and the initial concentration of E2 is the only difference between cultures of each group. BM culture is a kind of bacteria culture with 100 mg/L of NH4-N as only nitrogen source. Every group’s N- NH4+, N- NO3-concentration and OD600 were measured. The result shows that compared with the control group, in which no E2 was added, the growth of heterotrophic nitrifying bacteria had been promoted when the concentration of E2 was in range of 1 - 100 ng/L. In addition, heterotrophic nitrifying bacteria’s growing speed has a positive correlation between the E2’s concentration. However, low concentration of E2 (like 0.1 ng/L), could inhibit the growth of heterotrophic nitrifying bacteria. Considering the impact of E2 on heterotrophic nitrifying bacteria, it is necessary to intensify the detection of E2 in the future.
- Published
- 2020
45. DETECT: Development of Technologies for Early HCC Detection
- Author
-
Jihane N. Benhammou, Nicole E. Rich, George Cholankeril, Peng Zhang, Weihua Zeng, Shuyun Rao, Nabihah Tayob, Wenyuan Li, Xiaoli Wu, Shuo Feng, Naoto Fujiwara, Xiaoqing Meng, Shijia Zhu, and Sobia Zaidi
- Subjects
Carcinoma, Hepatocellular ,Early Diagnosis ,Hepatology ,Liver Neoplasms ,Gastroenterology ,Biomarkers, Tumor ,Humans ,alpha-Fetoproteins ,Early Detection of Cancer ,Article - Published
- 2021
46. cfTrack: Exome-wide mutation analysis of cell-free DNA to simultaneously monitor the full spectrum of cancer treatment outcomes: MRD, recurrence, and evolution
- Author
-
Edward B. Garon, Shuo Li, Adam Neal, Xiaohui Ni, Xianghong Jasmine Zhou, Weihua Zeng, Sanaz Memarzadeh, Zorawar S. Noor, Steven M. Dubinett, Wenyuan Li, Yonggang Zhou, Mary L. Stackpole, and Zuyang Yuan
- Subjects
Oncology ,medicine.medical_specialty ,business.industry ,Cancer ,Second primary cancer ,medicine.disease ,Minimal residual disease ,Deep sequencing ,Cancer treatment ,Cell-free fetal DNA ,Internal medicine ,medicine ,Mutation testing ,business ,Exome - Abstract
PurposeCell-free DNA (cfDNA) offers a non-invasive approach to monitor cancer. Here we develop a method using whole-exome sequencing (WES) of cfDNA for simultaneously monitoring the full spectrum of cancer treatment outcomes, including MRD, recurrence, evolution, and second primary cancer.Experimental DesignThree simulation datasets were generated from 26 cancer patients to benchmark the detection performance of MRD/recurrence and second primary cancers. For further validation, cfDNA samples (n=76) from cancer patients (n=35) with six different cancer types were used for validating the performance of cancer monitoring during various treatments.ResultsWe present a cfDNA-based cancer monitoring method, named cfTrack. Taking advantage of the broad genome coverage of WES data, cfTrack can sensitively detect MRD and cancer recurrence by integrating signals across the known clonal tumor mutations of a patient. In addition, cfTrack detects tumor evolution and second primary cancers by de novo identifying emerging tumor mutations. A series of machine learning and statistical denoising techniques are applied to enhance the detection power. On the simulation data, cfTrack achieved an average AUC of 99% on the validation dataset and 100% on the independent dataset in detecting recurrence in samples with tumor fraction ≥0.05%. In addition, cfTrack yielded an average AUC of 88% in detecting second primary cancers in samples with tumor fraction ≥0.2%. On real data, cfTrack accurately monitors tumor evolution during treatment, which cannot be accomplished by previous methods.ConclusionOur results demonstrated that cfTrack can sensitively and specifically monitor the full spectrum of cancer treatment outcomes using exome-wide mutation analysis of cfDNA.Translational RelevanceContinuous cancer monitoring is clinically necessary for cancer patients to detect minimal residual disease (MRD), recurrence, and progression, allowing for early intervention and therapy adjustment. Cell-free DNA (cfDNA) in blood has become an appealing option due to its non-invasiveness. Until now, cfDNA-based cancer monitoring methods have been focused on deep sequencing at a few known mutations, which are however insufficient when tumors evolve or new tumors emerge. We present the method, cfTrack, which for the first time uses whole-exome sequencing (WES) of cfDNA to track the full range of cancer treatment outcomes, including MRD, recurrence, evolution, and second primary cancer. We demonstrate that, even with very low tumor fractions, cfTrack achieves sensitive and specific monitoring of tumor MRD/recurrence/evolution based on both simulation data and a cohort of cancer patients. These findings demonstrate the clinical utility of cfTrack.
- Published
- 2021
47. Boundary delineation and grading functional zoning of Sanjiangyuan National Park based on biodiversity importance evaluations
- Author
-
Bingran Ma, Weihua Zeng, Yuxi Xie, Zhengzao Wang, Guanzheng Hu, Qing Li, Ruoxin Cao, Yue Zhuo, and Tongzuo Zhang
- Subjects
China ,Conservation of Natural Resources ,Environmental Engineering ,Parks, Recreational ,Environmental Chemistry ,Biodiversity ,City Planning ,Pollution ,Waste Management and Disposal ,Ecosystem - Abstract
Recently, China advanced a policy of establishing a protected area system with national parks as the main body, and so the development of protected areas has entered a transitional period. However, to promote the coordinated development of economic construction and ecological protection of protected areas, their management needs to be more comprehensive and refined. Therefore, a more quantitative and refined spatial planning and management method for protected areas is urgently needed. This study took Sanjiangyuan National Park as the research object and considered the three biodiversity elements of species, ecosystems and landscape. The maximum entropy (MaxEnt) model, Integrated Valuation of Ecosystem Services and Tradeoffs (InVEST) model, and Fragstats software were used to determine the areas with highly suitable species habitat, areas of high ecosystem service value and areas of high landscape diversity. Based on these areas, Marxan software was used to calculate the irreplaceability value, identify the boundary of Sanjiangyuan National Park and clarify the gaps of the existing Sanjiangyuan National Park in the space. We suggest that at least 18,569 km
- Published
- 2021
48. Construction of a human-wildlife spatial interaction index in the Three-River Source Region, China
- Author
-
Yuxi Xie, Tongzuo Zhang, Yinglan Xue, Bingran Ma, and Weihua Zeng
- Subjects
Herbivore ,Ecology ,business.industry ,Environmental resource management ,Wildlife ,General Decision Sciences ,Context (language use) ,Spatial distribution ,Geography ,River source ,Livestock ,Human impact intensity ,Species habitat suitability ,China ,business ,Baseline (configuration management) ,Three-River Source Region ,Ecology, Evolution, Behavior and Systematics ,QH540-549.5 ,Human-wildlife interaction - Abstract
In the context of today’s rapid economic development and increasingly strict ecological protection, interactions between humans and wildlife will intensify. Therefore, we need to identify the spatial distribution of such interactions and to take measures to mitigate possible negative interactions. Using the Three-Rivers Source Region as a case study, we combined species habitat suitability, species feeding habits, species conservation levels and human impact intensity to construct a “human-wildlife spatial interaction index” (HWSII) to evaluate the intensity of human-wildlife spatial interactions. The overall level of HWSII was relatively low in the Three-River Source Region. Higher potential human-wildlife interactions occurred in areas with roads and railways. Areas of relatively high interactions between carnivores and livestock were mainly located in the southern part of the study area, and those with relatively high interactions between herbivores and livestock appeared in the western and eastern parts. Managers in these areas should consider mitigation measures. Although developed for a specific region of China, the ‘human-wildlife spatial interaction index’ could have application in other areas of the world for setting a baseline reference to identify areas with the potential for high levels of human-wildlife interactions and pinpoint places where potential negative interactions can be mitigated.
- Published
- 2021
49. A <scp>high‐gain</scp> bow‐tie antenna with phase gradient metasurface lens
- Author
-
Xiao Zhao, Linyan Guo, Weihua Zeng, Minjie Guo, Ziye Wang, and Zhengwei Yang
- Subjects
Physics ,High-gain antenna ,business.industry ,Bow tie ,Computer Graphics and Computer-Aided Design ,Computer Science Applications ,law.invention ,Lens (optics) ,Optics ,law ,Phase gradient ,Electrical and Electronic Engineering ,Antenna (radio) ,business - Published
- 2021
50. Spatiotemporal variation and influencing factors of nitrogen and phosphorus in lake sediments in China since 1850
- Author
-
Yue Zhuo, Weihua Zeng, Bingran Ma, Dan Cui, Yuxi Xie, and Jianping Wang
- Subjects
Renewable Energy, Sustainability and the Environment ,Strategy and Management ,Building and Construction ,Industrial and Manufacturing Engineering ,General Environmental Science - Published
- 2022
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