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Over the past decade, although China's Internet industry has been keeping rapid development, however, the relevant laws and rules lag behind. Because of economic restructuring, aged tendency of population, industrial integration, and the gloomy employment of college students, it is urgent that China make a new paint for undergraduates create their new adventure under cyberspace. Nonetheless, implicit cost of online entrepreneurship, lagging entrepreneurship education and training, inflexible financial innovation, the influence of online credit environment and rules, abusive and inappropriate Internet regulations, are baffling Chinese undergraduates establishing their online start-ups. It is very crucial to optimize network entrepreneurial environment for these small websites and e-shops. With the era of web2.0 coming, subject and object is keeping evolution in cyberspace, entrepreneurial environment is faced with many new variables. In order to better the surrounding for entrepreneurs, government should work together with banks, portals, intermediary, traditional enterprises, small and medium entrepreneurs in case incompatible regulation poses a serious risk to undergraduate-based online start-ups. In the light of this goal, Chinese government should help third-party electronic commerce(EC) platforms build a new rule, which can promote effective competition for all kinds of communities in web2.0 ecosystem through more humanistic networks regulations. Universities ought to richen their entrepreneurial curricula system and construct a modern logistic support system for student start-ups online. It is also indispensable that intermediaries exert their functions during incubating undergraduate-based small business.

To evaluate the effect of calcium ionophore (CI) A23187 and human recombinant granulocyte/macrophage colony stimulating factor (rhGM-CSF) on the cultivation of dendritic cell (DC) from healthy human peripheral blood mononuclear cell (PBMC) and to evaluate the in vitro effect of DC stimulated by K562 cell lysate on inducing specific cytotoxic T lymphocyte (CTL) against K562 cell.Human PBMCs isolated from healthy subjects were separated into two groups. In Group A, the cells were cultured with additional rhGM-CSF, recombinant human interleukin 4 and recombinant human tumor necrosis factor-α only as control group. In Group B, the cells were cultured in the presence of rhGM-CSF and CI A23187. The cells in both groups were pre-incubated with K562 cell lysate at 37°C for 30 min. The cells were harvested after a 4-day cultivation. Morphology of DC was continuously observed under inverted microscope. The surface antigens of induced cells were analyzed by flow cytometry (FCM). Then the proliferation of allogeneic T cell and the specific cytotoxicity of T cell primed with DC were examined by colorimetry. Also, the nonspecific inhibition of DC loaded K562 cell lysate against K562 cell was detected.Typical morphological features of DC could be observed in both groups. The expressions of CD83, CD1a, CD86 and CD40 were stronger in Group B than those in control group (45.2% ± 1.8%, 31.5% ± 3.9%, 40.1% ± 7.8%, 36.4% ± 6.3% vs 16.9% ± 1.3%, 20.4% ± 3.4%, 26.5% ± 2.2%, 22.3% ± 3.0%) (all P0.05). The expression of CD14 was weaker in Group B than that in control group (5.7% ± 0.8% vs 19.0% ± 1.6%) (P0.05). As compared with the control group, DC in Group B loaded with K562 lysate could evidently stimulate the proliferation of allogeneic T cell (P0.05, exclusion of effector-to-target ratio of 1:40) and inhibit the growth of K562 cell (P0.05). In addition, both groups of DC-stimulated CTL had specific cytotoxicity against K562 cell. At the effector-to-target ratios of 10:1 and 40:1, the DC-stimulated CTL of Group B had stronger cytotoxicity against K562 cell (both P0.05).In combination with rhGM-CSF, CI A23187 induces PBMC into DC in a more effective way. DC loaded with K562 lysate can stimulate CTL and maintain high immunocompetence with specific cytotoxicity against K562 cell.

Using the advantages of Japanese encephalitis live attenuated and inactivated vaccine, to reduce the rate of immunization reaction and to increase the effect, we conducted a study on the strategy of immunization in Japanese encephalitis using live attenuated vaccine combined with inactivated vaccine.Observing the safety and immune effects of different groups.Data on side effect showed that the rate of moderate and severe systematic reactions of the group who were inoculated with combined vaccine was 0.73%, with local reaction 1.46% while the combined rate of moderate and severe systematic reaction of the group who were inoculated with inactivated vaccine was 2.8%. Under the detection of serum neutralizing antibody, the GMT rose from 1:1.05 - 1:3.35 before vaccination to 1:47.34 - 1:101.30 after vaccination in the different groups. Neutralizing antibody was detected in 97.67% of the combined group. There was a significant difference by comparing neutralizing antibody seroconversion rate of the combined group with the inactivated group (chi(2) = 3.89, P0.05), but no significant difference with attenuated group (chi(2) = 0.74, P0.05).Results showed that in children who previously had been immunized with two doses of inactivated vaccine, the booster administration of live attenuated vaccine was both effective and safe.