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2. Analysis of Characteristic Quality Indexes of Gaoyou Duck Eggs

Author

CHEN Mengying, GONG Lan, HE Tao, ZHU Lei, LUAN Fengting, DOU Weisheng, SHAO Xuemei,FANG Xiaomin, YOU Zhaorong, WEI Ruicheng, WANG Ran

Subjects
gaoyou duck eggs, conventional egg quality indicators, basic nutritional indicators, metabolomics, Food processing and manufacture, TP368-456
Abstract

To explore the quality characteristics of Gaoyou duck eggs, metabolomics was used to analyze the quality indexes, basic nutritional indexes and small molecule metabolites. The results showed that the mass, egg shape index, egg yolk color, Haugh unit, egg white height, egg yolk index and crude protein content of Gaoyou duck eggs were higher than those of the other studied breeds. The contents of total amino acids, umami amino acids, branched-chain amino acids, total fatty acids and unsaturated fatty acids were higher, and the content of saturated fatty acids was lower, indicating that sensory quality parameters, amino acids and fatty acids can characterize the quality of Gaoyou duck eggs. The characteristic components of Gaoyou duck eggs were uncovered by metabolomic fingerprinting, principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), and hierarchical cluster analysis (HCA). Totally 252 compounds and 22 differential compounds were identified in egg yolk, and 184 compounds and 40 differential compounds in egg white. Among them, uridine 5’-monophosphate, guanosine 5’-monophosphate, and N-acetyl-D-glucosamine were the main differential nutrients, and arachidonic acid in egg yolk was the unique nutrient of Gaoyou duck eggs, while indole was not detected in the egg white of Gaoyou duck eggs.

Published
2024
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9. Emergence of plasmid-mediated high-level tigecycline resistance genes in animals and humans

Author

He, Tao, Wang, Ran, Liu, Dejun, Walsh, Timothy R., Zhang, Rong, Lv, Yuan, Ke, Yuebin, Ji, Quanjiang, Wei, Ruicheng, Liu, Zhihai, Shen, Yingbo, Wang, Gang, Sun, Lichang, Lei, Lei, Lv, Ziquan, Li, Yun, Pang, Maoda, Wang, Liyuan, Sun, Qiaoling, Fu, Yulin, Song, Huangwei, Hao, Yuxin, Shen, Zhangqi, Wang, Shaolin, Chen, Gongxiang, Wu, Congming, Shen, Jianzhong, and Wang, Yang

Published
2019
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16. Dual function of Langerhans cells in skin TSLP-promoted TFH differentiation in mouse atopic dermatitis

Author

Marschall, Pierre, primary, Wei, Ruicheng, additional, Segaud, Justine, additional, Yao, Wenjin, additional, Hener, Pierre, additional, German, Beatriz Falcon, additional, Meyer, Pierre, additional, Hugel, Cecile, additional, Ada Da Silva, Grace, additional, Braun, Reinhard, additional, Kaplan, Daniel H., additional, and Li, Mei, additional

Published
2021
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22. Staphylococcus aureus Bacteriophage Suppresses LPS-Induced Inflammation in MAC-T Bovine Mammary Epithelial Cells

Author

Zhang, Lili, Hou, Xiang, Sun, Lichang, He, Tao, Wei, Ruicheng, Pang, Maoda, and Wang, Ran

Subjects
0301 basic medicine, Microbiology (medical), Staphylococcus aureus, LPS, 030106 microbiology, lcsh:QR1-502, Microbiology, NF-κB, lcsh:Microbiology, 03 medical and health sciences, MAC-T cells, 030104 developmental biology, bacteriophage, inflammation, Original Research
Abstract

Several previous studies have shown that bacteriophages can significantly affect the production of various cytokines. The aim of this present study was to investigate the inflammatory effects and mechanisms of bacteriophage vB_SauM_JS25 in stimulated MAC-T bovine mammary epithelial cells by real-time polymerase chain reaction (PCR) and Western blotting. Experiments show that vB_SauM_JS25 reduces Staphylococcus aureus- or lipopolysaccharide (LPS)-induced levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, IL-8, IL-10, and regulated on activation, normal T cell expressed and secreted (RANTES) mRNA in MAC-T cells, in a manner expected to be unrelated to its antibacterial action. Moreover, S. aureus bacteriophage vB_SauM_JS25 suppressed the LPS-induced phosphorylation of nuclear factor (NF)-κB p65, which may represent an important mechanism mediating these effects. A carefully regulated balance between activation and inhibition by bacteriophages must be kept avoiding inappropriate inflammatory responses. The ability of vB_SauM_JS25 to influence the immune response highlights the potential development and application of bacteriophage-based therapies and may represent a novel anti-inflammatory therapeutic strategy.

Published
2018

28. Study of the mechanisms underlying the type 2 immune response in atopic dermatitis pathogenesis

Author

Wei, Ruicheng, Institut de Génétique et de Biologie Moléculaire et Cellulaire ( IGBMC ), Université de Strasbourg ( UNISTRA ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Université de Strasbourg, Mei Li, STAR, ABES, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), and Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Germinal center formation, Formation des centres germinatifs, Dermatite atopique, [SDV.IMM] Life Sciences [q-bio]/Immunology, Cellules dendritiques, [SDV.IMM]Life Sciences [q-bio]/Immunology, Cellules Tfh, [ SDV.IMM ] Life Sciences [q-bio]/Immunology, Tfh cells, [ SDV.MHEP.DERM ] Life Sciences [q-bio]/Human health and pathology/Dermatology, Dendritic cells, Atopic dermatitis
Abstract

My thesis aimed at studying the Tfh cell differentiation, function and regulation in AD pathogenesis. To this aim, I employed our previously established AD mouse model in which MC903 (a vitamin D analog) topical treatment on the skin induces TSLP production bykeratinocytes, promotes Th2 cell response and drives the pathogenesis of AD. my thesis work investigated Tfh cell differentiation, its cytokine expression and germinal center formation using MC903-induced AD mouse model. By exploring the role of TSLP,Langerin+ DCs and OX40L signaling in Tfh cell differentiation and regulation, my study provides novel insights into the mechanisms underlying the type 2 immune response in AD pathogenesis. In the second part of my study, we examined the role of MC903 in regulating the psoriatic inflammation using Aldara-induced psoriasis model. We showed that MC903 inhibited IL-23/IL-17/IL-22 axis in mouse psoriasis. Moreover, this inhibition exhibited a dose-dependent manner. We further explored the role of TSLP and VDR in mediating such effect of MC903., Mon travail, lors de ma thèse, avait pour but d’étudier la différentiation des lymphocytes Tfh, ainsi que leur fonction et leur régulation dans la pathogenèse de la DA. Pour cela, j’ai utilisé un modèle murin précédemment établi au sein de notre laboratoire consistant en l’application topique de MC903 (un analogue de la vitamine D3) induisant la production de TSLP par les kératinocytes et, par conséquence, la réponse immunitaire Th2 et la pathogenèse de la DA. mon travail doctoral s’est porté sur la différentiation des lymphocytes Tfh, leur production cytokinique ainsi que la formation des centres germinatifs dans le contexte d’un modèle murin de DA induite par le MC903. Mes études ont démontré un rôle critique joué par TSLP dans la réponse Tfh et ont exploré le rôle potentiellement joué par les cellules dendritiques langerine+ et la signalisation OX40L dans le développement des réponses Tfh et de type 2. Ceci nous a permis d’approfondir nos connaissances concernant les mécanismes sous-tendant la réponse immunitaire de type 2 dans la pathogenèse de la DA. Dans la deuxième partie de ma thèse, nous avons examiné le rôle de MC903 dans la régulation de l’inflammation due au psoriasis, en utilisant un modèle de psoriasis induit par l’Aldara. Nous avons montré que MC903 inhibe l’axe 23/IL-17/IL-22 chez les souris souffrant de psoriasis. De plus, cette inhibition semblait être dose-dépendante. Nous avons en outre exploré le rôle de TSLP et VDR dans la médiation de cet effet dû au MC903.

Published
2016

35. The Cytokine and Chemokine Profiles in Patients with Hand, Foot and Mouth Disease of Different Severities in Shanghai, China, 2010

Author

Zeng, Mei, primary, Zheng, Xiaoyan, additional, Wei, Ruicheng, additional, Zhang, Na, additional, Zhu, Kai, additional, Xu, Bin, additional, Yang, Chun-Hui, additional, Yang, Chun-Fu, additional, Deng, Chaoyang, additional, Pu, Dongbo, additional, Wang, Xiaohong, additional, Altmeyer, Ralf, additional, and Leng, Qibin, additional

Published
2013
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36. HCV J6/JFH1 Tilts the Capability of Myeloid-Derived Dendritic Cells to Favor the Induction of Immunosuppression and Th17-Related Inflammatory Cytokines

Author

Fang, Zhong, primary, Zhu, Kai, additional, Guo, Nining, additional, Zhang, Na, additional, Guan, Mo, additional, Yang, Chunfu, additional, Pan, Qinsong, additional, Wei, Ruicheng, additional, Yang, Chunhui, additional, Deng, Chaoyang, additional, Liu, Xiaoqing, additional, Zhao, Ping, additional, and Leng, Qibin, additional

Published
2013
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43. High-Performance Liquid Chromatographic Method for the Determination of Cyromazine and Melamine Residues in Milk and Pork.

Author

Wei, Ruicheng, Wang, Ran, Zeng, Qingfei, Chen, Ming, and Liu, Tiezheng

Subjects
*CYROMAZINE, *MELAMINE, *HIGH performance liquid chromatography, *ACETONITRILE, *MILK analysis, *PORK, *SOLID phase extraction
Abstract

A high-performance liquid chromatography method is described in this paper. The method uses NH2 column and 97% acetonitrile eluate to determine the insecticide cyromazine and metabolite melamine residues in milk and pork. Samples were treated with NaOH and extracted with acetonitrile containing 20% NH4OH. Target analytes of samples were cleaned up and concentrated by C18 column solid-phase extraction. A separation for cyromazine and melamine was achieved, and respective retention times were 8 and 12 min. The calibration curves for cyromazine and melamine were linear in a concentration range of 0.01-1.0 µg/mL, with correlation coefficients of 0.9999 and 0.9997, respectively. The limit of detection of both compounds was 0.2 ng, and the limit of quantitation was 0.02 mg/kg. Recoveries of cyromazine and melamine at fortified levels of 0.02, 0.05, and 0.1 mg/kg ranged from 84.5-90.8%, and 83.6-91.3%, respectively, with coefficient of variation of 3.1-7.8%. [ABSTRACT FROM AUTHOR]

Published
2009
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44. Intracellular Staphylococcus aureusControl by Virulent Bacteriophages within MAC-T Bovine Mammary Epithelial Cells

Author

Zhang, Lili, Sun, Lichang, Wei, Ruicheng, Gao, Qiang, He, Tao, Xu, Cunfa, Liu, Xianjin, and Wang, Ran

Abstract

ABSTRACTBacteriophages (phages) are known to effectively kill extracellular multiplying bacteria. The present study demonstrated that phages penetrated bovine mammary epithelial cells and cleared intracellular Staphylococcus aureusin a time-dependent manner. In particular, phage vB_SauM_JS25 reached the nucleus within 3 h postincubation. The phages had an endocytotic efficiency of 12%. This ability to kill intracellular host bacteria suggests the utility of phage-based therapies and may protect patients from recurrent infection and treatment failure.

Published
2016
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45. Dual function of Langerhans cells in skin TSLP-promoted T FH differentiation in mouse atopic dermatitis.

Author

Marschall P, Wei R, Segaud J, Yao W, Hener P, German BF, Meyer P, Hugel C, Ada Da Silva G, Braun R, Kaplan DH, and Li M

Subjects
Allergens immunology, Animals, Calcitriol analogs & derivatives, Calcitriol pharmacology, Cell Differentiation, Dermatologic Agents pharmacology, Gene Expression Profiling, Mice, Inbred BALB C, Mice, Transgenic, Ovalbumin immunology, Thymic Stromal Lymphopoietin, Mice, Cytokines immunology, Dermatitis, Atopic immunology, Langerhans Cells immunology, Skin immunology, T-Lymphocytes, Helper-Inducer immunology
Abstract

Background: Atopic dermatitis (AD) is among the most common chronic inflammatory skin diseases, usually occurring early in life, and often preceding other atopic diseases such as asthma. T H 2 has been believed to play a crucial role in cellular and humoral response in AD, but accumulating evidence has shown that follicular helper T cell (T FH ), a critical player in humoral immunity, is associated with disease severity and plays an important role in AD pathogenesis., Objectives: This study aimed at investigating how T FH s are generated during the pathogenesis of AD, particularly what is the role of keratinocyte-derived cytokine TSLP and Langerhans cells (LCs)., Methods: Two experimental AD mouse models were employed: (1) triggered by the overproduction of TSLP through topical application of MC903, and (2) induced by epicutaneous allergen ovalbumin (OVA) sensitization., Results: This study demonstrated that the development of T FH s and germinal center (GC) response were crucially dependent on TSLP in both the MC903 model and the OVA sensitization model. Moreover, we found that LCs promoted T FH differentiation and GC response in the MC903 model, and the depletion of Langerin + dendritic cells (DCs) or selective depletion of LCs diminished the T FH /GC response. By contrast, in the model with OVA sensitization, LCs inhibited T FH /GC response and suppressed T H 2 skin inflammation and the subsequent asthma. Transcriptomic analysis of Langerin + and Langerin - migratory DCs revealed that Langerin + DCs became activated in the MC903 model, whereas these cells remained inactivated in OVA sensitization model., Conclusions: Together, these studies revealed a dual functionality of LCs in TSLP-promoted T FH and T H 2 differentiation in AD pathogenesis., (Copyright © 2020 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.)

Published
2021
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