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Your search keyword '"Wedderburn-Pugh, Kaylee"' showing total 9 results
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9 results on '"Wedderburn-Pugh, Kaylee"'

1. Proinflammatory immune cells disrupt angiogenesis and promote germinal matrix hemorrhage in prenatal human brain

Author

Chen, Jiapei, Crouch, Elizabeth E., Zawadzki, Miriam E., Jacobs, Kyle A., Mayo, Lakyn N., Choi, Jennifer Ja-Yoon, Lin, Pin-Yeh, Shaikh, Saba, Tsui, Jessica, Gonzalez-Granero, Susana, Waller, Shamari, Kelekar, Avani, Kang, Gugene, Valenzuela, Edward J., Birrueta, Janeth Ochoa, Diafos, Loukas N., Wedderburn-Pugh, Kaylee, Di Marco, Barbara, Xia, Wenlong, Han, Claudia Z., Coufal, Nicole G., Glass, Christopher K., Fancy, Stephen P. J., Alfonso, Julieta, Kriegstein, Arnold R., Oldham, Michael C., Garcia-Verdugo, Jose Manuel, Kutys, Matthew L., Lehtinen, Maria K., Combes, Alexis J., and Huang, Eric J.

Published
2024
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2. Profiling human brain vascular cells using single-cell transcriptomics and organoids.

Author

Crouch, Elizabeth, Diafos, Loukas, Valenzuela, Edward, Wedderburn-Pugh, Kaylee, Birrueta, Janeth, Caston, Jaela, Joseph, Tara, Andrews, Madeline, Bhaduri, Aparna, and Huang, Eric

Subjects
Humans, Organoids, Neurogenesis, Gene Expression Profiling, Transcriptome, Brain
Abstract

Angiogenesis and neurogenesis are functionally interconnected during brain development. However, the study of the vasculature has trailed other brain cell types because they are delicate and of low abundance. Here we describe a protocol extension to purify prenatal human brain endothelial and mural cells with FACS and utilize them in downstream applications, including transcriptomics, culture and organoid transplantation. This approach is simple, efficient and generates high yields from small amounts of tissue. When the experiment is completed within a 24 h postmortem interval, these healthy cells produce high-quality data in single-cell transcriptomics experiments. These vascular cells can be cultured, passaged and expanded for many in vitro assays, including Matrigel vascular tube formation, microfluidic chambers and metabolic measurements. Under these culture conditions, primary vascular cells maintain expression of cell-type markers for at least 3 weeks. Finally, we describe how to use primary vascular cells for transplantation into cortical organoids, which captures key features of neurovascular interactions in prenatal human brain development. In terms of timing, tissue processing and staining requires ~3 h, followed by an additional 3 h of FACS. The transplant procedure of primary, FACS-purified vascular cells into cortical organoids requires an additional 2 h. The time required for different transcriptomic and epigenomic protocols can vary based on the specific application, and we offer strategies to mitigate batch effects and optimize data quality. In sum, this vasculo-centric approach offers an integrated platform to interrogate neurovascular interactions and human brain vascular development.

Published
2024

3. Ensembles of endothelial and mural cells promote angiogenesis in prenatal human brain

Author

Crouch, Elizabeth E, Bhaduri, Aparna, Andrews, Madeline G, Cebrian-Silla, Arantxa, Diafos, Loukas N, Birrueta, Janeth Ochoa, Wedderburn-Pugh, Kaylee, Valenzuela, Edward J, Bennett, Neal K, Eze, Ugomma C, Sandoval-Espinosa, Carmen, Chen, Jiapei, Mora, Cristina, Ross, Jayden M, Howard, Clare E, Gonzalez-Granero, Susana, Lozano, Jaime Ferrer, Vento, Maximo, Haeussler, Maximilian, Paredes, Mercedes F, Nakamura, Ken, Garcia-Verdugo, Jose Manuel, Alvarez-Buylla, Arturo, Kriegstein, Arnold R, and Huang, Eric J

Subjects
Biochemistry and Cell Biology, Biomedical and Clinical Sciences, Biological Sciences, Neurosciences, Stem Cell Research, Pediatric, 1.1 Normal biological development and functioning, 2.1 Biological and endogenous factors, Brain, Collagen, Endothelial Cells, Humans, Laminin, Midkine, Neovascularization, Pathologic, Neovascularization, Physiologic, Pericytes, angiogenesis, arterial endothelial cells, blood brain barrier, cortical organoids, endothelial cells, human prenatal brain development, mural cells, pericytes, smooth muscle cells, tip cells, venous and capillary endothelial cells, ventricular zone, Human prenatal brain development, Medical and Health Sciences, Developmental Biology, Biological sciences, Biomedical and clinical sciences
Abstract

Interactions between angiogenesis and neurogenesis regulate embryonic brain development. However, a comprehensive understanding of the stages of vascular cell maturation is lacking, especially in the prenatal human brain. Using fluorescence-activated cell sorting, single-cell transcriptomics, and histological and ultrastructural analyses, we show that an ensemble of endothelial and mural cell subtypes tile the brain vasculature during the second trimester. These vascular cells follow distinct developmental trajectories and utilize diverse signaling mechanisms, including collagen, laminin, and midkine, to facilitate cell-cell communication and maturation. Interestingly, our results reveal that tip cells, a subtype of endothelial cells, are highly enriched near the ventricular zone, the site of active neurogenesis. Consistent with these observations, prenatal vascular cells transplanted into cortical organoids exhibit restricted lineage potential that favors tip cells, promotes neurogenesis, and reduces cellular stress. Together, our results uncover important mechanisms into vascular maturation during this critical period of human brain development.

Published
2022

4. Targeting ryanodine receptor type 2 to mitigate chemotherapy-induced neurocognitive impairments in mice

Author

Liu, Yang, primary, Reiken, Steven, additional, Dridi, Haikel, additional, Yuan, Qi, additional, Mohammad, Khalid S., additional, Trivedi, Trupti, additional, Miotto, Marco C., additional, Wedderburn-Pugh, Kaylee, additional, Sittenfeld, Leah, additional, Kerley, Ynez, additional, Meyer, Jill A., additional, Peters, Jonathan S., additional, Persohn, Scott C., additional, Bedwell, Amanda A., additional, Figueiredo, Lucas L., additional, Suresh, Sukanya, additional, She, Yun, additional, Soni, Rajesh Kumar, additional, Territo, Paul R., additional, Marks, Andrew R., additional, and Guise, Theresa A., additional

Published
2023
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5. Ensembles of endothelial and mural cells promote angiogenesis in prenatal human brain.

Author

Bhaduri, Aparna, Bhaduri, Aparna, Andrews, Madeline, Cebrian-Silla, Arantxa, Diafos, Loukas, Birrueta, Janeth, Wedderburn-Pugh, Kaylee, Valenzuela, Edward, Bennett, Neal, Eze, Ugomma, Sandoval-Espinosa, Carmen, Mora, Cristina, Ross, Jayden, Howard, Clare, Gonzalez-Granero, Susana, Lozano, Jaime, Vento, Maximo, Garcia-Verdugo, Jose, Huang, Eric, Kriegstein, Arnold, Alvarez-Buylla, Arturo, Nakamura, Ken, Paredes, Mercedes, Haeussler, Maximilian, Crouch, Elizabeth, Chen, Jiapei, Bhaduri, Aparna, Bhaduri, Aparna, Andrews, Madeline, Cebrian-Silla, Arantxa, Diafos, Loukas, Birrueta, Janeth, Wedderburn-Pugh, Kaylee, Valenzuela, Edward, Bennett, Neal, Eze, Ugomma, Sandoval-Espinosa, Carmen, Mora, Cristina, Ross, Jayden, Howard, Clare, Gonzalez-Granero, Susana, Lozano, Jaime, Vento, Maximo, Garcia-Verdugo, Jose, Huang, Eric, Kriegstein, Arnold, Alvarez-Buylla, Arturo, Nakamura, Ken, Paredes, Mercedes, Haeussler, Maximilian, Crouch, Elizabeth, and Chen, Jiapei

Abstract

Interactions between angiogenesis and neurogenesis regulate embryonic brain development. However, a comprehensive understanding of the stages of vascular cell maturation is lacking, especially in the prenatal human brain. Using fluorescence-activated cell sorting, single-cell transcriptomics, and histological and ultrastructural analyses, we show that an ensemble of endothelial and mural cell subtypes tile the brain vasculature during the second trimester. These vascular cells follow distinct developmental trajectories and utilize diverse signaling mechanisms, including collagen, laminin, and midkine, to facilitate cell-cell communication and maturation. Interestingly, our results reveal that tip cells, a subtype of endothelial cells, are highly enriched near the ventricular zone, the site of active neurogenesis. Consistent with these observations, prenatal vascular cells transplanted into cortical organoids exhibit restricted lineage potential that favors tip cells, promotes neurogenesis, and reduces cellular stress. Together, our results uncover important mechanisms into vascular maturation during this critical period of human brain development.

Published
2022

6. Proinflammatory Milieu Disrupts Homeostatic Microglia-Vascular Interactions to Promote Germinal Matrix Hemorrhage

Author

Chen, Jiapei, primary, Crouch, Elizabeth Erin, additional, Waller, Shamari, additional, Kelekar, Avani, additional, Kang, Gugene, additional, Diafos, Loukas N., additional, Wedderburn-Pugh, Kaylee, additional, Di Marco, Barbara, additional, Xia, Wenlong, additional, Valenzuela, Edward J., additional, Han, Claudia Z., additional, Coufal, Nicole G., additional, Glass, Christopher K., additional, Fancy, Stephen PJ, additional, Alfonso, Julieta, additional, Oldham, Michael C., additional, and Huang, Eric, additional

Published
2022
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7. Ensembles of Endothelial and Mural Cells Promote Angiogenesis in Prenatal Human Brain

Author

Crouch, Elizabeth Erin, primary, Bhaduri, Aparna, additional, Andrews, Madeline G., additional, Cebrian-Silla, Arantxa, additional, Diafos, Loukas N., additional, Birrueta, Janeth Ochoa, additional, Wedderburn-Pugh, Kaylee, additional, Eze, Ugomma C., additional, Sandoval-Espinosa, Carmen, additional, Chen, Jiapei, additional, Mora, Cristina, additional, Ross, Jayden M., additional, Howard, Clare E., additional, Gonzalez-Granero, Susana, additional, Ferrer Lozano, Jaime, additional, Vento, Maximo, additional, Haeussler, Maximilian, additional, Paredes, Mercedes F., additional, García Verdugo, Jose Manuel, additional, Alvarez-Buylla, Arturo, additional, Kriegstein, Arnold R., additional, and Huang, Eric, additional

Published
2022
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8. Intracellular calcium leak as a therapeutic target for RYR1-related myopathies

Author

Kushnir, Alexander, primary, Todd, Joshua J., additional, Witherspoon, Jessica W., additional, Yuan, Qi, additional, Reiken, Steven, additional, Lin, Harvey, additional, Munce, Ross H., additional, Wajsberg, Benjamin, additional, Melville, Zephan, additional, Clarke, Oliver B., additional, Wedderburn-Pugh, Kaylee, additional, Wronska, Anetta, additional, Razaqyar, Muslima S., additional, Chrismer, Irene C., additional, Shelton, Monique O., additional, Mankodi, Ami, additional, Grunseich, Christopher, additional, Tarnopolsky, Mark A., additional, Tanji, Kurenai, additional, Hirano, Michio, additional, Riazi, Sheila, additional, Kraeva, Natalia, additional, Voermans, Nicol C., additional, Gruber, Angela, additional, Allen, Carolyn, additional, Meilleur, Katherine G., additional, and Marks, Andrew R., additional

Published
2020
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