43 results on '"Wawrzyńska, Magdalena"'
Search Results
2. Three-Dimensional-Printed Modular Titanium Alloy Plates for Osteosynthesis of the Jawbone.
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Dobrzyński, Maciej, Szymonowicz, Maria, Nowicka, Joanna, Pajączkowska, Magdalena, Nikodem, Anna, Kuropka, Piotr, Wawrzyńska, Magdalena, and Rusak, Agnieszka
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ALLOY plating ,TITANIUM alloys ,THREE-dimensional printing ,LACTOBACILLUS rhamnosus ,SURFACE plates - Abstract
Backgrounds: The titanium–aluminum–vanadium alloy (Ti-6Al-4V) is frequently used in implantology due to its biocompatibility. The use of 3D printing enables the mechanical modification of implant structures and the adaptation of their shape to the specific needs of individual patients. Methods: The titanium alloy plates were designed using the 3D CAD method and printed using a 3D SLM printer. Qualitative tests were performed on the material surface using a microcomputed tomography scanner. The cytotoxicity of the modular titanium plates was investigated using the MTT assay on the L929 cell line and in direct contact with Balb/3T3 cells. Cell adhesion to the material surface was evaluated with hFOB1.19 human osteoblasts. Microbial biofilm formation was investigated on strains of Lactobacillus rhamnosus, Staphylococcus epidermidis, Streptococcus mutans and Candida albicans using the TTC test and scanning electron microscopy (SEM). Results: The surface analysis showed the hydrophobic nature of the implant. The study showed that the titanium plates had no cytotoxic properties. In addition, the material surface showed favorable properties for osteoblast adhesion. Among the microorganisms tested, the strains of S. mutans and S. epidermidis showed the highest adhesion capacity to the plate surface, while the fungus C. albicans showed the lowest adhesion capacity. Conclusions: The manufactured modular plates have properties that are advantageous for the implantation and reduction in selected forms of microbial biofilm. Three-dimensional-printed modular titanium plates were investigated in this study and revealed the potential clinical application of this type of materials, regarding lack of cytotoxicity, high adhesion properties for osteoblasts and reduction in biofilm formation. The 3D CAD method allows us to personalise the shape of implants for individual patients. [ABSTRACT FROM AUTHOR]
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- 2024
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3. The Use of Graphene Oxide in Orthodontics—A Systematic Review
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Rygas, Joanna, primary, Matys, Jacek, additional, Wawrzyńska, Magdalena, additional, Szymonowicz, Maria, additional, and Dobrzyński, Maciej, additional
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- 2023
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4. Palmitic Acid Modulates Microglial Cell Response to Metabolic Endotoxemia in an In Vitro Study
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Chmielarz, Mateusz, primary, Sobieszczańska, Beata, additional, Teisseyre, Andrzej, additional, Wawrzyńska, Magdalena, additional, Bożemska, Edyta, additional, and Środa-Pomianek, Kamila, additional
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- 2023
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5. Analysis of COVID-19 Incidence and Protective Potential of Persisting IgG Class Antibodies against SARS-CoV-2 Infection in Hospital Staff in Poland
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Radziejewska, Jadwiga, primary, Arkowski, Jacek, additional, Susło, Robert, additional, Kędzierski, Kamil, additional, and Wawrzyńska, Magdalena, additional
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- 2023
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6. The Role of Palmitic Acid in the Co-Toxicity of Bacterial Metabolites to Endothelial Cells
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Choroszy, Marcin, primary, Środa-Pomianek, Kamila, additional, Wawrzyńska, Magdalena, additional, Chmielarz, Mateusz, additional, Bożemska, Edyta, additional, and Sobieszczańska, Beata, additional
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- 2023
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7. Inhibition of MMP-2 expression affects metabolic enzyme expression levels: Proteomic analysis of rat cardiomyocytes
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Lin, Han-Bin, Sharma, Keshav, Bialy, Dariusz, Wawrzynska, Magdalena, Purves, Randy, Cayabyab, Francisco S., Wozniak, Mieczyslaw, and Sawicki, Grzegorz
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- 2014
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8. Photoactivated titania-based nanomaterials for potential application as cardiovascular stent coatings
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Kopaczyńska, Marta, Sobieszczańska, Beata, Ulatowska-Jarża, Agnieszka, Hołowacz, Iwona, Buzalewicz, Igor, Wasyluk, Łukasz, Tofail, Syed A.M., Biały, Dariusz, Wawrzyńska, Magdalena, and Podbielska, Halina
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- 2014
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9. Telemedicine in Cardiology: Modern Technologies to Improve Cardiovascular Patients’ Outcomes—A Narrative Review
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Kędzierski, Kamil, primary, Radziejewska, Jadwiga, additional, Sławuta, Agnieszka, additional, Wawrzyńska, Magdalena, additional, and Arkowski, Jacek, additional
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- 2022
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10. Co-toxicity of Endotoxin and Indoxyl Sulfate, Gut-Derived Bacterial Metabolites, to Vascular Endothelial Cells in Coronary Arterial Disease Accompanied by Gut Dysbiosis
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Choroszy, Marcin, primary, Sobieszczańska, Beata, additional, Litwinowicz, Kamil, additional, Łaczmański, Łukasz, additional, Chmielarz, Mateusz, additional, Walczuk, Urszula, additional, Roleder, Tomasz, additional, Radziejewska, Jadwiga, additional, and Wawrzyńska, Magdalena, additional
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- 2022
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11. Intracoronary Application of TiO2-Coated Cardiovascular Stents
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Wawrzyńska, Magdalena, primary, Kopaczyńska, Marta, additional, Sobieszczańska, Beata, additional, Ulatowska-Jarża, Agnieszka, additional, Hołowacz, Iwona, additional, Biały, Dariusz, additional, Pasławska, Urszula, additional, Wasyluk, Łukasz, additional, Tofail, Syed A. M, additional, Bauer, Joanna, additional, and Podbielska, Halina, additional
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- 2016
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12. In situ photoexcitation of silver-doped titania nanopowders for activity against bacteria and yeasts
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Kowal, Katarzyna, Wysocka-Król, Katarzyna, Kopaczyńska, Marta, Dworniczek, Ewa, Franiczek, Roman, Wawrzyńska, Magdalena, Vargová, Melinda, Zahoran, Miroslav, Rakovský, Erik, Kuš, Peter, Plesch, Gustav, Plecenik, Andrej, Laffir, Fathima, Tofail, Syed A.M., and Podbielska, Halina
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- 2011
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13. Production, Mechanical Properties and Biomedical Characterization of ZrTi-Based Bulk Metallic Glasses in Comparison with 316L Stainless Steel and Ti6Al4V Alloy
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Hasiak, Mariusz, primary, Sobieszczańska, Beata, additional, Łaszcz, Amadeusz, additional, Biały, Michał, additional, Chęcmanowski, Jacek, additional, Zatoński, Tomasz, additional, Bożemska, Edyta, additional, and Wawrzyńska, Magdalena, additional
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- 2021
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14. Co-toxicity of Endotoxin and Indoxyl Sulfate, Gut-Derived Bacterial Metabolites, to Vascular Endothelial Cells in Coronary Arterial Disease Accompanied by Gut Dysbiosis
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Choroszy, Marcin, primary, Sobieszczanska, Beata, additional, Litwinowicz, Kamil, additional, Laczmanski, Lukasz, additional, Chmielarz, Mateusz, additional, Walczuk, Urszula, additional, Roleder, Tomasz, additional, Radziejewska, Jadwiga, additional, and Wawrzyńska, Magdalena, additional
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- 2021
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15. In Vitro Photodynamic Therapy with Chlorin e6 Leads to Apoptosis of Human Vascular Smooth Muscle Cells
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Wawrzyńska, Magdalena, Kałas, Wojciech, Biały, Dariusz, Zioło, Ewa, Arkowski, Jacek, Mazurek, Walentyna, and Strządała, Leon
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- 2010
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16. Graphene Coating Obtained in a Cold-Wall CVD Process on the Co-Cr Alloy (L-605) for Medical Applications
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Wasyluk, Łukasz, primary, Boiko, Vitalii, additional, Markowska, Marta, additional, Hasiak, Mariusz, additional, Saladino, Maria Luisa, additional, Hreniak, Dariusz, additional, Amati, Matteo, additional, Gregoratti, Luca, additional, Zeller, Patrick, additional, Biały, Dariusz, additional, Arkowski, Jacek, additional, and Wawrzyńska, Magdalena, additional
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- 2021
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17. Comparison of Osmotic Resistance, Shape and Transmembrane Potential of Erythrocytes Collected from Healthy and Fed with High Fat-Carbohydrates Diet (HF-CD) Pigs—Protective Effect of Cistus incanus L. Extracts
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Cyboran-Mikołajczyk, Sylwia, primary, Pasławski, Robert, additional, Pasławska, Urszula, additional, Nowak, Kacper, additional, Płóciennik, Michał, additional, Męczarska, Katarzyna, additional, Oszmiański, Jan, additional, Bonarska-Kujawa, Dorota, additional, Kowalczyk, Paweł, additional, and Wawrzyńska, Magdalena, additional
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- 2021
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18. Impact of Liposomal Drug Formulations on the RBCs Shape, Transmembrane Potential, and Mechanical Properties
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Cyboran-Mikołajczyk, Sylwia, primary, Sareło, Przemysław, additional, Pasławski, Robert, additional, Pasławska, Urszula, additional, Przybyło, Magdalena, additional, Nowak, Kacper, additional, Płóciennik, Michał, additional, Podbielska, Halina, additional, Kopaczyńska, Marta, additional, and Wawrzyńska, Magdalena, additional
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- 2021
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19. Applications for graphene and its derivatives in medical devices: Current knowledge and future applications
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Arkowski, Jacek, primary, Obremska, Marta, additional, Kędzierski, Kamil, additional, Sławuta, Agnieszka, additional, and Wawrzyńska, Magdalena, additional
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- 2020
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20. Antibody CD133 Biofunctionalization of Ammonium Acryloyldimethyltaurate and Vinylpyrrolidone Co-Polymer-Based Coating of the Vascular Implants
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Sareło, Przemysław, primary, Duda, Maciej, additional, Gąsior-Głogowska, Marlena, additional, Wysokińska, Edyta, additional, Kałas, Wojciech, additional, Podbielska, Halina, additional, Wawrzyńska, Magdalena, additional, and Kopaczyńska, Marta, additional
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- 2020
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21. Graphene Oxide Carboxymethylcellulose Nanocomposite for Dressing Materials
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Saladino, Maria Luisa, primary, Markowska, Marta, additional, Carmone, Clara, additional, Cancemi, Patrizia, additional, Alduina, Rosa, additional, Presentato, Alessandro, additional, Scaffaro, Roberto, additional, Biały, Dariusz, additional, Hasiak, Mariusz, additional, Hreniak, Dariusz, additional, and Wawrzyńska, Magdalena, additional
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- 2020
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22. Photoactive Pore Matrix for In Situ Delivery of a Photosensitizer in Vascular Smooth Muscle Cells Selective PDT
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Wawrzyńska, Magdalena, primary, Duda, Maciej, additional, Hołowacz, Iwona, additional, Kaczorowska, Aleksandra, additional, Ulatowska-Jarża, Agnieszka, additional, Buzalewicz, Igor, additional, Kałas, Wojciech, additional, Wysokińska, Edyta, additional, Biały, Dariusz, additional, Podbielska, Halina, additional, and Kopaczyńska, Marta, additional
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- 2019
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23. Photoactive Liposomal Formulation of PVP-Conjugated Chlorin e6 for Photodynamic Reduction of Atherosclerotic Plaque
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Kałas, Wojciech, primary, Wysokińska, Edyta, additional, Przybyło, Magdalena, additional, Langner, Marek, additional, Ulatowska-Jarża, Agnieszka, additional, Biały, Dariusz, additional, Wawrzyńska, Magdalena, additional, Zioło, Ewa, additional, Gil, Wojciech, additional, Trzeciak, Anna M., additional, Podbielska, Halina, additional, and Kopaczyńska, Marta, additional
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- 2019
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24. Functionalization with a VEGFR2‐binding antibody fragment leads to enhanced endothelialization of a cardiovascular stent in vitro and in vivo
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Wawrzyńska, Magdalena, primary, Kraskiewicz, Honorata, additional, Paprocka, Maria, additional, Krawczenko, Agnieszka, additional, Bielawska‐Pohl, Aleksandra, additional, Biały, Dariusz, additional, Roleder, Tomasz, additional, Wojakowski, Wojciech, additional, O'Connor, Iain B., additional, Duda, Maciej, additional, Michal, Robert, additional, Wasyluk, Łukasz, additional, Plesch, Gustav, additional, Podbielska, Halina, additional, Kopaczyńska, Marta, additional, and Wall, J. Gerard, additional
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- 2019
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25. Functionalized CD133 antibody coated stent surface simultaneously promotes EPCs adhesion and inhibits smooth muscle cell proliferation–A novel approach to prevent in-stent restenosis
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Wawrzyńska, Magdalena, primary, Duda, Maciej, additional, Wysokińska, Edyta, additional, Strządała, Leon, additional, Biały, Dariusz, additional, Ulatowska-Jarża, Agnieszka, additional, Kałas, Wojciech, additional, Kraszewski, Sebastian, additional, Pasławski, Robert, additional, Biernat, Paweł, additional, Pasławska, Urszula, additional, Zielonka, Aleksandra, additional, Podbielska, Halina, additional, and Kopaczyńska, Marta, additional
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- 2019
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26. Biocompatible Carbon-Based Coating as Potential Endovascular Material for Stent Surface
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Wawrzyńska, Magdalena, primary, Bil-Lula, Iwona, additional, Krzywonos-Zawadzka, Anna, additional, Arkowski, Jacek, additional, Łukaszewicz, Mikołaj, additional, Hreniak, Dariusz, additional, Stręk, Wiesław, additional, Sawicki, Grzegorz, additional, Woźniak, Mieczysław, additional, Drab, Marek, additional, Frączkowska, Kaja, additional, Duda, Maciej, additional, Kopaczyńska, Marta, additional, Podbielska, Halina, additional, and Biały, Dariusz, additional
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- 2018
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27. Functionalization with a VEGFR2‐binding antibody fragment leads to enhanced endothelialization of a cardiovascular stent in vitro and in vivo.
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Wawrzyńska, Magdalena, Kraskiewicz, Honorata, Paprocka, Maria, Krawczenko, Agnieszka, Bielawska‐Pohl, Aleksandra, Biały, Dariusz, Roleder, Tomasz, Wojakowski, Wojciech, O'Connor, Iain B., Duda, Maciej, Michal, Robert, Wasyluk, Łukasz, Plesch, Gustav, Podbielska, Halina, Kopaczyńska, Marta, and Wall, J. Gerard
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VASCULAR endothelial growth factors ,PROGENITOR cells ,RECOMBINANT antibodies ,ENDOTHELIAL cells ,UMBILICAL veins ,VASCULAR endothelial growth factor antagonists - Abstract
Rapid endothelialization of cardiovascular stents is critical to prevent major clinical complications such as restenosis. Reconstruction of the native endothelium on the stent surface can be achieved by the capture of endothelial progenitor cells (EPCs) or neighboring endothelial cells (ECs) in vivo. In this study, stainless steel cardiovascular stents were functionalized with recombinant scFv antibody fragments specific for vascular endothelial growth factor receptor‐2 (VEGFR2) that is expressed on EPCs and ECs. Anti‐VEGFR2 scFvs were expressed in glycosylated form in Escherichia coli and covalently attached to amine‐functionalized, titania‐coated steel disks and stents. ScFv‐coated surfaces exhibited no detectable cytotoxicity to human ECs or erythrocytes in vitro and bound 15 times more VEGFR2‐positive human umbilical vein ECs than controls after as little as 3 min. Porcine coronary arteries were successfully stented with scFv‐coated stents with no adverse clinical events after 30 days. Endovascular imaging and histology revealed coverage of the anti‐VEGFR2 scFv‐coated stent with a cell layer after 5 days and the presence of a neointima layer with a minimum thickness of 80 μm after 30 days. Biofunctionalization of cardiovascular stents with endothelial cell‐capturing antibody fragments in this manner offers promise in accelerating stent endothelialization in vivo. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:213–224, 2020. [ABSTRACT FROM AUTHOR]
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- 2020
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28. Low frequency electromagnetic field decreases ischemia–reperfusion injury of human cardiomyocytes and supports their metabolic function
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Biały, Dariusz, primary, Wawrzyńska, Magdalena, additional, Bil-Lula, Iwona, additional, Krzywonos-Zawadzka, Anna, additional, Sapa-Wojciechowska, Agnieszka, additional, Arkowski, Jacek, additional, Woźniak, Mieczysław, additional, and Sawicki, Grzegorz, additional
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- 2018
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29. Characterization of restenotic tissue depending on the prevalence of neovascularization evaluated by optical coherence tomography in porcine artery model
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Tokarek, Tomasz, Wysocki, Tomasz, Siudak, Zbigniew, Wiktorowicz, Agata, Legutko, Jacek, Żmudka, Krzysztof, Dudek, Dariusz, Dziewierz, Artur, Rakowski, Tomasz, Błachut, Aleksandra, Pruski, Maciej, Janas, Adam, Michalak, Magdalena, Milewski, Krzysztof, Buszman, Piotr P., Buszman, Paweł E., Roleder, Tomasz, Dobrolińska, Magdalena, Pociask, Elżbieta, Wańha, Wojciech, Parma, Radosław, Smolka, Grzegorz, Ochała, Andrzej, Gąsior, Zbigniew, Tendera, Michał, Wojakowski, Wojciech, Kleczyński, Paweł, Bagieński, Maciej, Rzeszutko, Łukasz, Bartuś, Stanisław, Zimoch, Wojciech, Kosowski, Michał, Kübler, Piotr, Tomasiewicz, Brunon, Telichowski, Artur, Reczuch, Krzysztof, Krzysztofik, Justyna, Langner, Anna, Żurakowski, Aleksander, Szykowski, Dariusz, Kachel, Mateusz, Kosmulski, Kamil, Nowakowski, Przemysław, Daniec, Marzena, Sorysz, Danuta, Trębacz, Jarosław, Tomala, Marek, Nawrotek, Bartłomiej, Henzel, Jan, Rudziński, Piotr N., Kłopotowski, Mariusz, Konka, Marek, Dzielińska, Zofia, Demkow, Marcin, Rzeźnik, Daniel, Rosławiecka, Agnieszka, Kabłak-Ziembicka, Anna, Misztal, Marcin, Maciejewski, Damian, Pieniążek, Piotr, Trystuła, Mariusz, Przewłocki, Tadeusz, Badacz, Rafał, Dzierwa, Karolina, Piatek, Jacek, Konstanty-Kalandyk, Janusz, Paluszek, Piotr, Tekieli, Łukasz, Trystula, Mariusz, Michalski, Michał, Musial, Robert, Urbańczyk-Zawadzka, Małgorzata, Banyś, Robert P., Musiałek, Piotr, Karch, Izabela, Konkolewska, Magda, Bailey, Charles E., Kiesz, R. Stefan, Trendel, Wojciech, Biały, Dariusz, Woźna, Katarzyna, Wawrzyńska, Magdalena, and Arkowski, Jacek
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Abstracts of original contributions: 9-P ,Abstracts of original contributions: 8-P ,Abstracts of original contributions: 15-P ,Abstracts of original contributions: 16-P ,Abstracts of original contributions: 13-P ,Abstracts of original contributions: 14-P ,Abstracts of original contributions: 17-P ,Abstracts of original contributions: 18-P ,Abstracts of original contributions: 11-P ,Abstracts of original contributions: 12-P ,Abstracts of original contributions: 10-P ,Abstracts of Original Contributions ,Abstracts of original contributions: 5-P ,Abstracts of original contributions: 4-P ,cardiovascular diseases ,Abstracts of original contributions: 7-P ,Abstracts of original contributions: 6-P ,Abstracts of original contributions: 1-P ,Abstracts of original contributions: 3-P ,Abstracts of original contributions: 2-P - Abstract
Background The utilization of the radial access (RA) for percutaneous coronary intervention (PCI) has gradually increased. Recent studies suggest an advantage over femoral access (FA) in high-risk patients presenting with ST-segment elevation myocardial infarction (STEMI). The aim was to evaluate bleeding complications and periprocedural outcomes with safety and efficacy of RA compared with FA for PCI with stent implantation in “real-world” patients with STEMI presentation from Polish National Registry. Methods The study group consisted of 22,812 consecutive patients with STEMI treated with PCI and stent implantation between January 2014 and June 2015 in 151 tertiary invasive cardiology centers in Poland. All data were stored in electronic database of National PCI Registry (ORPKI). Patients treated with RA and FA were compared using a propensity score analysis to best match between groups. The analysis was done in the “as-treated” manner. Results Femoral access and RA were used in 9,334 (40.9%) and 13,478 (59.1%) patients, respectively. After propensity score matching no differences in baseline characteristic between 6,542 matched pairs was found. Significantly higher total amount of contrast (191.8 ±8 ml vs. 174.8 ±68.8 ml, p = 0.0001) and lower radiation doses were used in FA (1279.5 ±1346.3 mGy vs. 1182.6 ±887 mGy, p = 0.02). More bleeding complications at puncture site after both angiography (0.17% vs. 0.02%; p = 0.004) and PCI (0.23% vs. 0.09%, p = 0.049) were reported in FA group. Periprocedural death (1.94% vs. 0.93%, p = 0.0001) and periprocedural cardiac arrest (1.44% vs. 0.96%, p = 0.01) occurred significantly more often after PCI performed with FA. Conclusions Radial access was associated with lower incidence of periprocedural death in STEMI patients as well as a significant reduction of bleeding complications at access site., Background Little is known on the impact of psoriasis on the outcome of percutaneous cardiovascular procedures. The aim of this analysis was to identify the incidence of active psoriasis, baseline characteristic, and periprocedural to in-hospital outcomes in this subgroup of patients. Methods The Polish National PCI database (ORPKI) is a mandatory registry for all percutaneous cardiology procedures performed in Poland since 2004. All consecutive patients who had coronary angiography or coronary angiography with immediate PCI in 155 interventional cardiology centers in Poland in 2014 for either stable angina or acute coronary syndrome were included. Patients with active psoriasis on admission were identified. Allergic origin of the periprocedural complication was defined if a typical reaction from rash to anaphylaxis was diagnosed. Results There were 206,335 patients with complete records in the database. Active psoriasis was diagnosed in 830 of them (0.4%). Patients with psoriasis were younger (63.1 ±10.6 years vs. 66.2 ±10.9 years, p < 0.01), significantly more often with diabetes mellitus, arterial hypertension and chronic kidney disease. The periprocedural mortality for patients with psoriasis (0.77%) was similar to those without (0.55%; p = 0.6). Interestingly, allergic reaction occurrence during angiography was 0.77% in patients with psoriasis vs. 0.07% in patients without psoriasis (p < 0.001). In multivariate regression analysis, the diagnosis of active psoriasis was an independent predictor of the occurrence of an allergic reaction during coronary angiography/PCI with OR = 8.3 and 95% CI: 1.98–34.93 (p = 0.004). Conclusions Psoriasis is associated with a different patient baseline profile and an increased risk for allergic reaction occurrence during the procedure. Best to our knowledge, this is the first report of such relationship and requires further study., Background Neovascularization in restenotic tissue may play a key role in neointimal proliferation and progression of neoatherosclerosis. There are still insufficient in vivo data regarding in-stent neovascularization. Methods We evaluated 48 stent segments from 24 stents implanted in porcine model of coronary artery injury. Optical coherence tomography (OCT) was performed 90 days post implantation. All measurements and qualitative analysis were performed by two independent investigators. Microvessels were defined as well delineated low backscattering structures with diameter less than 200 microns that show a trajectory within the vessel. There were two groups: with (1) or without (2) occurrence of neovascularization. Optical coherence tomography findings were compared between both groups. Results There were no statistically significant differences between the two groups, but lesions with microvessels had a larger average stent area (8.83 ±1.39 vs. 8.27 ±1.36, p = NS) and larger average neointimal area (2.62 ±1.46 vs. 1.95 ±0.87, p = NS) than those without microvessels. Minimal lumen cross-sectional area (CSA) were almost identical in both groups (2.56 ±0.47 vs. 2.57 ±0.33, p = NS). Conclusions Occurrence of neovascularization might be associated with the extent of neointimal area, but further studies are required to estimate factors associated with strut neovascularization., Background The previous study using intravascular imaging presented that the part of the treated atheroma is squeezed out of the vessel by the stent and its remnants may embolize. The present study aimed to characterize the plaque redistribution with relation to its composition. Methods The combined near-infrared spectroscopy (NIRS) and intravascular ultrasound (IVUS) imaging analyzed the plaque distribution after the stent implantation in vivo in patients with a stable coronary artery disease (CAD) (n = 33) and acute coronary syndromes (n = 16). Near-infrared spectroscopy detected lipids in the lesion and IVUS presented the redistribution of the plaque. The maximal lipid core burden index in a 4 mm long segment (LCBI4mm) was estimated by NIRS before and after the stent implantation, and IVUS estimated minimal lumen area (MLA), plaque burden (PB) and plaque volume pre and post implantation. Results NIRS-IVUS imaging was performed before and after the implantation of 50 stents in 49 patients. The median plaque volume pre and post implantation was 127.45 (74.5, 186.8) mm2 vs. 99.35 (67.5, 171.5) mm2 (p < 0.001) with a median difference 19.25 (3.9, 72) mm2. The median LCBI4mm pre and post stent was 351 (157, 589) vs. 77 (3, 231) (p < 0.001) in the treated lesions, and 34 (0, 207) vs. 0 (0, 45) (p < 0.04) in segment 4 mm long proximally to the stent. Plaque burden in 4 mm proximally to the implanted stent increased from 46.23 ±11.79 to 50.36 ±9.93 post stenting (p = 0.02). The difference in the plaque volume pre and post stenting correlated with the value of LCBI4max of the treated lesion (r = 0.457, p = 0.001). The median difference in the plaque volume was higher for stented lesions with LCBI 4mm > 265 (8.2 (1.4, 19.3) vs. 24.3 (12.1, 45.0), p = 0.012) (Figure 1). Figure 1 Case example of the combined near-infrared spectroscopy and intravascular ultrasound imaging Conclusions Lipid-rich lesions characterized greater release of the atheroma remnants to the coronary artery lumen as compared to non-lipid rich lesions. The stent implantation causes plaque shift proximally to the implanted stent., Background Adenosine is commonly used to induce maximal hyperemia to evaluate the hemodynamic significance of coronary stenoses with measuring fractional flow reserve (FFR). So far, limited number of studies validated the intracoronary application of adenosine against standard intravenous infusion. Thus, we aimed to assess agreement and reproducibility of FFR measurements during intracoronary vs. intravenous administration of adenosine. Methods Consecutive patients with angiographically borderline coronary lesions (40–90%) diameter stenosis in visual assessment), who were scheduled for FFR were enrolled. Patients received intravenous adenosine infusion via a 6-F femoral venous sheath. Adenosine was administered at 140 at 280 μg/kg/min. Intracoronary adenosine bolus injection was performed with 100 μg, 200 μg, 400 μg and 600 μg. Results Femoral vein adenosine at 140 and 280 μg/ kg/min as well as intracoronary boluses recordings from 81 vessels in 32 patients were suitable for blinded analysis. The median FFR measured using adenosine administered via femoral routes at 140 μg/kg/min was 0.82 (IQR: 0.76–0.89) and at 280 μg/kg/min – 0.82 (IQR: 0.75–0.88), p = 0.001. However, a strong correlation between them was found r = 0.99. Median FFR values for 100 μg, 200 μg, 400 μg and 600 μg bolus were: 0.84, 0.83, 0.83 and 0.83, respectively. We found numerically higher values of FFR assessed with boluses comparing to infusion with the mean difference of FFR from 0.01 (bolus 600 μg) to 0.02 (bolus 100 μg). We did not find any differences between 400 and 600 μg bolus (p = 0.21). Differences between boluses and infusion are shown in details on Figure 1. Figure 1 Mean values of fractional flow reserve assessed with adenosine i.c. boluses and i.v. infusion Conclusions Values of FFR achieved with adenosine infusion via femoral vein and with intracoronary boluses are consistent. However, there seems to be a grey zone for FFR assessed with boluses which may indicate, in selected cases, the use of femoral vein infusion to confirm the results., Background Rotational atherectomy (RA) is often used as a bailout technique after unsuccessful percutaneous coronary intervention (PCI). This study was created to evaluate outcomes of patients undergoing RA depending on reason of previous PCI failure. Methods We retrospectively evaluated data of 210 consecutive patients undergoing RA in our tertiary center. From our analysis we excluded patients undergoing RA without previous PCI attempt (n = 42), those referred from other centers without description of PCI failure cause (n = 10) or lacking complete clinical data (n = 6). Remaining patients (n = 152) were divided into two groups: those with uncrossable (32%) or undilatable lesions (68%). Follow- up data concerning death from any cause was acquired from the Polish National Health Found. Results Patients with uncrossable lesions were older (72 vs. 69 years; p = 0.03) and more often suffered from stroke or transient ischemic attack in anamnesis (20% vs. 8%, p = 0.04). The groups did not differ in any other clinical characteristics. We also did not show any differences in procedure characteristics, success and complications rates or in-hospital adverse events. Patients with uncrossable lesions had higher all-cause mortality after 1 year (18% vs. 8%; p = 0.05), 2 years (24% vs. 10%; p = 0.02) and 3 years (29% vs. 14%; p = 0.03). Multivariate Cox regression model showed uncrossable coronary lesions to be an independent predicting factor of all-cause mortality 2 years after procedure (HR = 2.8; 95% CI: 1.13–7.15; p = 0.03). Conclusions Patients undergoing RA after failed PCI due to uncrossable lesions have poorer long term prognosis. Further studies evaluating causes of higher mortality in this population are needed., Background This study was created to assess the frequency and influence of severe coronary artery calcification (CAC) in target lesions on adverse cardiac events in patients undergoing percutaneous coronary interventions (PCI) due to acute myocardial infarction. Methods We prospectively evaluated data of 206 consecutive patients who underwent PCI due to acute myocardial infarction. The pre-specified angiographic definition of severe calcifications was the presence of radiopaque densities visible without heart motion and affecting both sides of the treated lesion. Follow-up data concerning primary end points was acquired from the Polish National Health Found. Results Severe CAC in target lesions were present in 35 (17%) patients. These patients were older (71 vs. 65 years, p = 0.02), were more often diagnosed with non-ST elevation myocardial infarction (NSTEMI, 77% vs. 58%, p = 0.03) and had higher levels of NT-proBNP at admission (2006 pg/ml vs. 745 pg/ml, p = 0.01). Full revascularization during index procedure was achieved less often in patients with severe CAC (14% vs. 41%, p = 0.003). During 30 months of follow-up patients with severe CAC more often suffered from another ACS (37% vs. 13%, p = 0.0005) and had higher all-cause mortality (31% vs. 16%, p = 0.04). Multivariate Cox regression model showed severe CAC in target lesions to be the only independent predicting factor of another ACS in studied population (hazard ratio = 2.78; 95% confidence interval: 1.39–5.56; p = 0.004). Conclusions In everyday practice, almost every sixth patient with myocardial infarction has severe CAC in target lesions. This group has poorer prognosis then the rest of the population. Presence of severe CAC in target lesions independently predicts recurrent ACS., Background Rotational atherectomy (RA) is s method of treatment of highly calcified lesions that cannot be treated with traditional percutaneous coronary intervention (PCI). However, RA in patients with acute coronary syndromes (ACS) remains controversial and may be contraindicated. Methods The study included 207 consecutive patients, who underwent RA. A retrospective analysis of data in subjects presenting with ACS (n = 42) or treated electively (n = 165) was done. Results Patients presenting with ACS were older, had higher systolic blood pressure and heart rate on admission, they were less likely to undergo PCI before and had lower total left ventricular ejection fraction (LVEF). Estimated risk and lesion complexity were higher in the ACS population. We found no differences in procedural aspects. We noted higher rates of contrast-induced nephropathy and bleeding in the ACS group. During in-hospital observation 2 cases of death occurred in the ACS group, but we found no difference in total mortality, ACS, decompensated HF or stroke rates in 1-year follow-up. Analysis of Cox hazard risk model revealed PCI failure due to uncrossable lesion to be risk factor of total 1-year mortality in the whole studied group; increasing LVEF improved the prognosis. Detailed data are presented in the Table I (see page 396). Table I General characteristics, risk factors and comorbidities Parameter All patients (n = 207) Elective (n = 165) ACS (n = 42) P-value Age, mean ± SD [years] 71 ±2 70 ±1 75 ±4 < 0.001 Previous ACS, n (%) 130 (63) 99 (60) 31 (74) NS Previous PCI, n (%) 152 (74) 128 (78) 24 (57) < 0.01 Previous CABG, n (%) 31 (15) 26 (15) 6 (14) NS Heart failure, n (%) 70 (34) 50 (30) 20 (48) < 0.05 LVEF, mean ± SD [%] 50 ±11 51 ±11 47 ±13 < 0.05 LVEF < 35%, n (%) 34 (17) 22 (14) 12 (29) < 0.05 Procedural data EuroScore II mortality rate, median (IQR) 2.4 (1.4-4.9) 2.1 (1.3-3.8) 5.15 (2.6-9.3) < 0.0001 Syntax Score, median (IQR) 17 (11-24) 17 (11-24) 21.5 (15-29) < 0.05 CABG inoperable, n (%) 116 (56) 86 (52) 30 (41) < 0.05 Attempted PCI failure, n (%): Nondilatable lesion 105 (51) 86 (52) 19 (45) NS Uncrossable lesion 48 (23) 38 (23) 10 (24) NS Unknown 11 (5) 9 (6) 2 (5) NS Procedure success, n (%) 184 (89) 147 (89) 37 (88) NS Ad hoc procedure, n (%) 6 (4) 7 (4) 2 (5) NS Radial access, n (%) 125 (60) 99 (60) 26 (62) NS GP IIb/IIIa inhibitor, n (%) 5 (2) 5 (3) 0 NS IABP n (%) 4 (2) 2 (1) 2 (5) NS Outcomes CIN, n (%) 6 (3) 2 (1) 4 (10) < 0.05 ARC major bleeding, n (%) 14 (7) 8 (5) 6 (14) < 0.05 In-hospital mortality, n (%) 2 (1) 0 2 (5) < 0.05 Inhospital stroke/TIA, n (%) 1 (0.5) 1 (1) 0 NS Inhospital MI, n (%) 28 (14) 22 (13) 6 (14) NS Inhospital TVR, n (%) 1 (0.5) 0 1 (2) NS Total 1-year mortality, n (%) 20 (10) 14 (8) 6 (14) NS 1-year MI, n (%) 20 (8) 15 (9) 5 (12) NS 1-year ADHF n (%) 12 (6) 9 (5) 3 (7) NS 1-year stroke/TIA, n (%) 2 (1) 2 (1) 0 NS Predictors of 1-year mortality (n = 207) Parameter HR 95% CI P-value LVEF (%) 0.95 0.91-0.99 < 0.05 PCI failure: uncrossable lesion 4.00 1.57-10.20 < 0.01 Conclusions One-fifth of RA candidates present with ACS. Despite higher periprocedural mortality rate in ACS group, we found no significant difference in 1-year outcomes in comparison with elective patients. Rotational atherectomy in patients with ACS in experienced centre should be considered in that particular setting if no other options of treatment exist., Background Some patients receiving dual antiplatelet therapy after percutaneous coronary interventions (PCI) with drug-eluting stent (DES) implantation require surgery to be performed during dual antiplatelet therapy (DAPT) administration period. Aim of our research was to assess the risk of premature discontinuation of DAPT after PCI with second generation DES implantation in patients undergoing major vascular surgeries. Methods Our study group consist of 88 patients who underwent PCI with second generation DES implantation between 2010 and 2014 and underwent major vascular surgery up to 12 months after PCI, requiring discontinuation of DAPT in perioperative period. We assessed the frequency of death, myocardial infarction (MI), major bleeding episodes and other adverse events in short and long-term observation. Results Patient characteristics are shown in Table I (see page 397). In perioperative period, we reported no MI, death, or major bleeding. During the follow up period of mean 2.71 (0.16–5.75) years, we recorded 14 (15.9%) deaths: 7 (7.9%) heart related, 4 (4.5%) cancer related, 3 (3.4%) others. In a multivariate analysis, the only independent factor of mortality was left ventricular ejection fraction (LVEF; OR = 0.93; 95 CI: 0.87–0.99; p = 0.02). Time between procedures was not a predictor of mortality and MI. Table I Patient characteristics Variables Values Study group 88 Mean age [years] 69 ±8.3 Sex, male 58 (66%) Mean time between procedures [days] 94.4 ±84.4 Diabetes 31 (35.2%) Mean EF 49.4% Arterial hypertension 73 (83.0%) Atrial fibrillation 8 (9.1%) Recent MI 32 (36.4%) PCI in history 31 (35.2%) CABG in history 9 (10.2%) PAD 67 (76.1%) Stenotic carotid arteries 23 (26.1%) Conclusions In patients with peripheral atherosclerosis requiring major vascular surgery after PCI with second generation DES implantation, early discontinuation of DAPT – on average 90 days after PCI is not associated with increased risk of death, MI, or major bleeding., Background This study aimed to evaluate gender-related differences in short- and long-term outcomes of patients undergoing balloon aortic valvuloplasty (BAV) for severe aortic stenosis (AS). Methods Between October 2012 and July 2015, a cohort of 112 patients with severe aortic AS underwent 114 BAV procedures as palliative procedure, bridge to definitive treatment or before urgent non-cardiac surgery. Clinical and procedural characteristics, in-hospital outcomes and 2-year mortality data were collected. Kaplan- Meier curves for women and men were constructed and compared by the log-rank test. Multivariable Cox regression analysis was performed to identify the independent predictors of long-term mortality. Results Of the 112 patients, 62.5% (n = 70) were women. As compared with men, women were older, had higher STS score, higher prevalence of chronic kidney disease, arterial hypertension and lower aortic valve area (all p < 0.05). Indications for BAV did not differ by gender. Women had higher risk of vascular complications than man (15.7% vs. 0.0%, p = 0.007) however with similar major periprocedural complications rate (17.1% vs. 9.5%, p = 0.40). Transcatheter aortic valve implantation (TAVI) was performed in 22.8% (n = 16) women and 26.2% (n = 11) men, p = 0.61, surgical aortic valve replacement (AVR) in 10% (n = 7) women and 11.9% (n = 5) men, p = 0.70. Women and men treated with TAVI/AVR had lower mortality as compared with conservative treatment (p = 0.004). Peri-procedural, in-hospital and 24- month mortality before definitive treatment were comparable between women and men (2.9% vs. 2.4%, p = 1.00; 11.4% vs. 4.9%, p = 0.26; 63.3% vs. 39.0%, p = 0.22, respectively). Kaplan-Meier curves for survival stratified by gender are shown in Figure 1. In a multivariable Cox model, STS above 9.8 (HR = 2.29; 95% CI: 1.09–4.83, p = 0.028) was an independent predictor of all-cause mortality only in women. Figure 1 Kaplan-Meier curves for survival stratified by gender Conclusions Presence of significant gender-related differences in baseline characteristics, procedural data and risk of vascular complications were confirmed for patients with severe AS undergoing BAV. However, no difference in major procedural complications as well as long-term mortality between women and men was observed., Background Patent foramen ovale (PFO) with occasional right-to-left shunt is associated with an increased risk of decompression illness (DCI). Divers with a history of repetitive or severe DCI and diagnosed PFO should be considered for the percutaneous closure of the defect if they wish to continue with unrestricted diving. In this study we aimed to summarise our experience in transcatheter PFO closure in professional divers with the history of DCI, based on a long-term follow-up of 11 divers treated in our centre. Methods A follow-up of 11 consecutive divers (9 males, 2 females) in whom percutaneous closure of PFO was performed between 2001–2015 for the secondary prevention of DCI was carried out by phone contact (Table I). Table I Characteristics of the investigated group No. Gender Age [years] Observation time [months] Occluder implanted Time from intervention to next diving [days] Number of divings after intervention Max. depth before intervention [m] Max. depth after intervention [m] 1 Male 41 172 APFO 25 5 10,000* 70 160 2 Female 36 121 APFO 25 7 100 110 70 3 Male 35 115 APFO 25 270 300 55 60 4 Male 32 101 APFO 25 180 700 115 120 5 Male 44 99 CARDIA PFO 30 90 800 60 40 6 Female 35 91 APFO 25 365 30 100 70 7 Male 44 60 APFO 25 90 350 75 90 8 Male 40 60 APFO 25 120 150 80 102 9 Male 49 58 APFO 25 30 1,000 140 140 10 Male 30 40 APFO 25 120 150 80 100 11 Male 47 9 APFO 25 180 30 102 80 * Saturation diving technique: total time (hours) from compression to complete decompression. Results The median follow-up at the time of the analysis was 91 (minimum 9, maximum 172) months. No complications of the procedure were observed in the investigated group. All patients returned to unrestricted, deep diving after the procedure, performing a total of 3,610 dives with the median number of 225 dives (lower quartile value: 82.5, upper quartile value: 72.5 dives). The majority of subjects dived as deep as they did before the intervention, or deeper, achieving mean maximum depth of 93.8 ±35.6 m (vs. 89.73 ±25.92 m before the intervention, p = 0.71). No episode of DCI was observed in the follow-up. Conclusions Percutaneous closure of PFO appears reasonably effective in secondary prevention of DCI associated with intra-cardiac shunt., Background In the light of randomized trials, renal artery stenting (PTA) seems to have questionable effect on clinical outcome. However, smaller observational studies indicated that still there might be a field for intervention in the selected subset of subjects. Therefore, the present study aimed to investigate what features distinguish subjects who improved in terms of the systolic blood pressure (SBP) reduction after PTA from those who did not benefit. Methods Study enrolled 118 (57 male, 63.9 ±8.8 years) consecutive patients referred to PTA for ill-controlled hypertension. Blood pressure control was obtained using 24-hour ambulatory blood pressure monitoring (ABPM), with assessment of the mean SBP and diastolic blood pressure (DBP), the loads of SBP and DBP in patients on antihypertensive drugs, before PTA and 12 months after PTA. Study group was divided retrospectively with regard to achieved SBP control. Subjects in whom SBP decreased by at least 20 mm Hg during 12 month FU after PTA were comprised in group I: 25 (21%) patients. Group II comprised 93 (79%) subjects in whom SBP reduction was lower than 20 mm Hg or deteriorated. Results Analysis showed that group I vs. group II patients had higher renal artery stenosis (RAS) degree (75.2 ±14.7% vs. 66.7 ±10.5%, p = 0.001). The initial SBP, DBP, SBP load and DBP load were significantly higher in group I vs. group II subjects (154.8 ±16.7 mm Hg vs. 130.6 ±16.4 mm Hg, p < 0.001; 86.1 ±11.7 mm Hg vs. 72.7 ±8.8 mm Hg, p < 0.001; 77.9 ±18.7 mm Hg vs. 40 ±28.2 mm Hg, p < 0.001, and 50 ±26.4 mm Hg vs. 21 ±18.5 mm Hg, p < 0.001, respectively). The mean number of blood pressure lowering agents was similar in group I and group II subjects, both before (3.44 ±1.3 vs. 3.26 ±1.1, p = 0.479) and 12 months following PTA (3.12 ±1.4 vs. 2.95 ±1.2, p = 0.541). This may indicate that the effect of blood pressure (BP) values decrease was associated rather with PTA, than drug regiments. On backward logistic multivariable analysis, independent predictors of significant blood pressure reduction after the PTA were: higher RAS degree (OR = 1.24, 95% CI: 1.07–1.44; p = 0.006), higher initial SBP (OR = 1.27, 95% CI: 1.03–1.56; p = 0.026), higher initial DBP load (OR = 1.33, 95% CI: 1.09–1.64; p = 0.007) and bilateral RAS or RAS of single functioning kidney (OR = 1.16, 95% CI: 1.00–1.36; p = 0.053). Conclusions Among patients after PTA, about 20% are high responders with regard to blood pressure control. Positive effect following PTA of RAS can be expected in patients with higher initial SBP and DBP parameters, with high-grade RAS and subjects with bilateral RAS or RAS of single functioning kidney. Our results indicate that PTA of RAS is justified in carefully selected patients., Background Studies indicate that patients with renal artery stenosis (RAS) are at high risk of cardiovascular events (CVE), e.g. myocardial infarction (MI), stroke (IS), and cardiovascular death (CVD). However, the impact of renal artery stenting (PTA) in subjects with RAS on the long-term prognosis and factors associated with this unfavourable prognosis are still debated. The present study aimed to evaluate impact of PTA on renal function, blood pressure and survival in patients treated with PTA for RAS, as well as to identify independent risk factors of CVE in long-term follow-up. Methods Two-hundred-and-forty renal artery angioplasty procedures were performed in 208 consecutive subjects (113 male, mean age: 64.5 ±11.9 years) for the mean RAS degree of 70.3 ±13.4% between June 1997 and December 2015. The immediate and long term outcome of PTA was assessed with regard to renal function, blood pressure values and incidence of restenosis. Prevalence of CVE, including CVD, MI, and IS were recorded prospectively. The potential prognostic risk factors of CVD/MI/IS were analyzed. Results The procedural success was 239/240 procedures. Mean creatinine level decreased from pre-PTA: 133 ±62 to 116 ±55.9 µmol/l at 6 month, and to 117 ±58.6 µmol/l at 12 months after PTA (p = 0.003). Systolic blood pressure changed from 142 ±21.8 mm Hg before PTA to 130 ±15.9 mm Hg, at 6 month, and to 130 ±15 mm Hg at 12 month (p < 0.001), while diastolic blood pressure from 78 ±12.3 to 74 ±9.2 mm Hg, and to 73.7 ±9.5 mm Hg (p < 0.001) respectively. The mean number of blood lowering agents was reduced from 3.33 ±1.2 before PTA to 3.06 ±1.3 at 12 month following PTA (p = 0.003). Restenosis occurred in 24/207 (11.6%) patients and it was treated with repeated PTA. Factors associated with restenosis were: hyperlipidemia (OR = 1.23, 95% CI: 1.06–1.42, p = 0.005) and diabetes (OR = 1.15, 95% CI: 1.0–1.33, p = 0.055). During the mean follow-up period of 62.1 ±37 (range: 1–213) months, CVE occurred in 54 (26%) patients (24 CVDs, 17 non-fatal MIs, 13 non-fatal ISs). Initial serum creatinine (OR = 1.25; 95% CI: 1.04–1.51, p = 0.02) and creatinine at 12 month (OR = 1.18; 95% CI: 1.03–1.35, p = 0.015), concomitant coronary artery disease (OR = 1.19; 95% CI: 1.04–1.36, p = 0.011), and SBP at 12 months after PTA (OR = 1.16; 95% CI: 1.02–1.33, p = 0.025) were independently associated with CVD/MI/IS risk. Conclusions PTA of RAS resulted in significant reduction of creatinine level, blood pressure values and the number of blood lowering agents. During mean 5-year period following PTA of RAS, the CVE rate was 26%. Factors independently associated with CVE risk were pre procedural and 12 month levels of serum creatinine, SBP at 12 months following PTA and concomitant coronary artery disease. Restenosis occurred in 11.6% and it was associated with hyperlipidemia and diabetes., Aim To evaluate the role of carotid artery stenting (CAS) performed according to the ‘tailored’ algorithm in the revascularization of severe internal carotid artery (ICA) stenosis in patients requiring (urgent or elective) cardiac surgery. Methods From 2009 to 2015, 100 patients (69.2 ± 6.7 years, range: 53–84 years; 77% male) underwent CAS combined with cardiac surgery: coronary artery bypass grafting (CABG) and/or valve surgery (Table I). The decision on the type and timing of the procedures was made individually in the vascular heart team. Patients with non-ST elevation myocardial infarction (NSTEMI)/unstable angina/NYHA III–IV had a hybrid procedure (HP): CAS simultaneous with cardiac surgery. Those with severe, but stable cardiac disease had staged procedure (SP): CAS in the first step and cardiac surgery ≥ 5 weeks later. Carotid artery stenting was done according to the ‘tailored’ strategy – to fit proper neuroprotection (proximal or distal) and stent type (open, closed-cell or mesh-covered) to the ICA stenosis severity and neurological symptoms. In the HP group CAS was performed on acetylsalicylic acid and unfractionated heparin; loading dose of clopidogrel (300 mg) was given in ≥ 6th postoperative hour, if major bleedings were excluded. Patients in SP group had dual antiplatelet therapy for a month, clopidogrel was withdrawn 5–7 days before cardiac surgery. Table I Patient and procedure characteristics Parameter HP (N = 58) SP (N = 42) P-value Age [years] 70.2 ±6.6, range: 53-83 67.9 ±6.8, range: 55-84 0.09 Ipsilateral stroke/TIA 17 (29%) 22 (52%) 0.13 ICA stenosis (%) 85.3 ±9.7; 60-99 85.3 ±8.1; 60-99 1.0 Proximal neuroprotection device 28 (48.3%) 21 (50%) 1.0 Closed-cell/mesh-covered stent 55 (95%) 37 (88%) 0.88 Left main stenosis 21 (36%) 12 (28.6%) 1.0 EuroSCORE II 2.74 ±1.39; 0.95-6.91 1.98 ±1.08; 0.85-4.95 0.004 Isolated CABG 52 (89.6%) 36 (86%) 0.88 Isolated valve surgery or CABG + other procedure* 6 (10.3%) 6 (12.5%) 0.75 * Valve/aortic surgery/left ventricle aneurysmectomy/ left atrial appendage closure. Results No major neurological in hospital or 30-day complications were noted. In the early postoperative period 1 myocardial infarction and 2 deaths as a result of multi-organ failure (3; 5.2%) occurred in the HP group and 1 (2.4%) death due to cardiogenic shock in SP group; p = 0.6. Conclusions Carotid artery stenting performed according to the ‘tailored’ algorithm prior to cardiac surgery is a low risk procedure and might be an option to prevent possible perioperative neurological complications of ICA stenosis. Patients requiring urgent cardiac surgery have slightly increased risk of the procedure., Background About 30% of patients with carotid artery stenosis (CAS) develop dementia after cerebral ischemic event (CIE), and 20–50% suffer from CIE recurrence during 6 months. Carotid artery revascularization (CAR) may prevent CIE recurrence, at the cost of new micro-embolic lesions (MES). The impact of CAR on cognitive function is debatable. The aim was to assess either functional and cognitive outcome, cerebral flow on transcranial Doppler (TCD) and brain magnetic resonance imaging (MRI) in patients with symptomatic CAS referred to CAR. Methods Fifteen patients (aged 68 ±8.2 years old, 9 male) with recent CIE (17.6 ±10.2 days to CAR) related to CAS of mean 88.9 ±3.9% lumen reduction were prospectively with TCD, diffusion and perfusion MRI, Montreal Cognitive Assessment (MoCA), Mini Mental State Examination (MMSE), modified Rankin Scale (mRs) and National Institutes of Health Stroke Scale (NIHSS) 24 h before, at 48–72 h and 1 month following CAR. Results New MES were found in 7 (46.7%) subjects following CAR. Carotid artery revascularization resulted in significant increase of cerebral flow velocity in middle and anterior cerebral arteries (p < 0.001 and p = 0.009; respectively) and cerebral perfusion measured by TTP and MTT (p = 0.005 and p = 0.002; respectively). Neurologic tests showed improvement in NIHSS (3.1 ±1.1 to 1.9 ±1.2; p = 0.003), mRs (from 1.6 ±0.8 to 0.9 ±0.9, p = 0.008), and MMSE (26.4 ±2.4 to 27.8 ±2.3; p = 0.012) at 1 month. Montreal Cognitive Assessment scores before and 1 month after CAR remained similar (22.8 ±3.6 vs. 23.4 ±3.3; p = 0.752). Conclusions Improvement of cerebral flow and perfusion, functional outcome, as well as no cognitive decline are observed after CAR for symptomatic CAS., Background Very early discharge after percutaneous coronary interventions (PCI) is now possible vascular closure devices (VCDs) when using the femoral access. Methods We retrospectively analyzed 261 consecutive procedures performed from femoral access with a VCD in a single center between January 2008 and December 2013. The primary endpoint was access site complication rate. The secondary endpoint was time from end of procedure to discharge. Results The complication rate was 2.68% (7/261). The following complications occurred: 3 hematomas, 1 pseudoaneurysm requiring treatment and 3 device failures. Mean time to discharge was 4 h 1 min (range: 2 h 19 min to 6 h 48 min). Conclusions Utilization of VCDs allows for safe and early same-day discharge of patients undergoing planned PCI procedures., Background Previous studies of same-day discharge after percutaneous coronary intervention (PCI) demonstrated favorable short term outcomes. There are limited data evaluated the long term results, especially for unselected population. Methods We retrospectively studied and followed up for 12 months 396 consecutive patients undergoing planned PCI with same-day discharge between December 2007 and May 2015 in two stand-alone centers. Results The mean age of the study population was 56.6 ±7.59 years (males 77.22%). 10.1% of the lesions were on bifurcation, 4.29% were CTO, 5.3% were in a bypass graft and 1.26% were in protected LM. Procedural success occurred in 96.21%. Groin access was used in 83.08% with VCD in 93.01% of procedures. Access site complications (uncomplicated VCD failure, hematoma, small dissection, pseudoaneurysm, spasms of radial) occurred in 1.77% of cases. Only 1 patient required hospitalization due to anaphylactic drug reaction in a peri-procedural period. The mean time from procedure end to discharge was 3 h 59 min. No death or major bleeding occurred during 30-day observation. Two patients had early stent thrombosis with MI, 1 patient was re-admitted for repeat angiography without intervention, 1 had TVR and one patient had stroke. At 12 months there were 15 ISR required angioplasty or CABG and 28 TVR. One patient had stroke and one had transient ischemic attack. Seven patients had MI at 12 months (1.77%). One patient died due to non-cardiac reasons. Conclusions Our study shows excellent long term safety outcomes for unselected population of patients undergoing elective PCI in stand-alone centers without overnight stay., Background We aimed to evaluate safety and efficacy of hemostatic polyurethane matrix (Hematrix) for femoral artery access site closure. Methods and results In a series of 31 patients two sizes of Hematrix patch were used: 10 × 10 cm (body mass index > 30 kg/m2 or thick layer of fat at puncture site) or 5 × 5 cm. The arterial sheath (6 F in all cases) was removed directly after the procedure and then the access site was manually compressed for 10 min. In patients who had PCI (and heparin administered) a compression band was placed for 6 h. In the remaining cases no compression band was used, and the access site was checked for hematoma or bleeding during the first 6 h. Hemostasis was obtained in all patients. The blood flow in femoral artery (evaluated by ultrasound examination) was normal in all cases. Only one serious complication was seen – a pseudoaneurysm of femoral artery access site – probably due to administration of low molecular weight heparin 48 h after the procedure. Two cases of mild complications (6% of the group) were seen: superficial hematomas that required prolonged compression by pressure band. Conclusions The Hematrix patch appears to be efficient in closing femoral artery access site after cornarography or PCI. It offers similar advantages and has equally low incidence of complications as currently available mechanical closure devices (sheath removed directly after the procedure, short bed rest or compression time). It is distinguished from them by much lower price and requires less training.
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- 2016
30. Efficiency of Chicken Blastoderm Cell Transfection Combining two Nonviral Methods: Electroporation and Synthetic Carriers
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Kinal, Anita, primary, Kozłowska, Izabela, additional, Łakota, Paweł, additional, Wawrzyńska, Magdalena, additional, and Kijewska, Ewelina, additional
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- 2017
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31. Calcium oxalate crystals in some Philodendron Schott (Araceae) species
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Klimko, Małgorzata, primary, Wawrzyńska, Magdalena, additional, and Wiland-Szymańska, Justyna, additional
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- 2016
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32. Subthreshold nitric oxide synthase inhibition improves synergistic effects of subthreshold MMP ‐2/ MLCK ‐mediated cardiomyocyte protection from hypoxic injury
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Bil‐Lula, Iwona, primary, Lin, Han‐Bin, additional, Biały, Dariusz, additional, Wawrzyńska, Magdalena, additional, Diebel, Lucas, additional, Sawicka, Jolanta, additional, Woźniak, Mieczyslaw, additional, and Sawicki, Grzegorz, additional
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- 2016
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33. Comparative leaf morphology and anatomy of some neotropical Philodendron Schott (Araceae) species
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Klimko, Małgorzata, primary, Wawrzyńska, Magdalena, additional, and Wiland-Szymańska, Justyna, additional
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- 2015
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34. Occurrence of Melampyrum cristatum L. in Dębno upon Warta (Wielkopolska, Poland)
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Czarna, Aneta, primary, Klimko, Małgorzata, additional, and Wawrzyńska, Magdalena, additional
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- 2015
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35. Multimodality imaging of intermediate lesions: Data from fractional flow reserve, optical coherence tomography, near-infrared spectroscopy-intravascular ultrasound.
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Biały, Dariusz, Wawrzyńska, Magdalena, Arkowski, Jacek, Rogała, Marcin, Proniewska, Klaudia, Wańha, Wojciech, Wojakowski, Wojciech, and Roleder, Tomasz
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- 2018
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36. Multimodality imaging of intermediate lesions: Data from FFR, OCT, NIRS-IVUS
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Biały, Dariusz, primary, Wawrzyńska, Magdalena, additional, Arkowski, Jacek, additional, Rogała, Marcin, additional, Proniewska, Klaudia, additional, Wańha, Wojciech, additional, Wojakowski, Wojciech, additional, and Roleder, Tomasz, additional
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- 2013
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37. Identification of the Rate of Chimerism of Different Tissues with Microsatellite Markers in Chicken Chimeras
- Author
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Siwek, Maria, primary, Sławińska, Anna, additional, Łakota, Paweł, additional, Grajewski, Bartosz, additional, Wawrzyńska, Magdalena, additional, Wiśniewska, Ewa, additional, Pławski, Andrzej, additional, Słomski, Ryszard, additional, and Bednarczyk, Marek, additional
- Published
- 2010
- Full Text
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38. Production, Mechanical Properties and Biomedical Characterization of ZrTi-Based Bulk Metallic Glasses in Comparison with 316L Stainless Steel and Ti6Al4V Alloy.
- Author
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Hasiak, Mariusz, Sobieszczańska, Beata, Łaszcz, Amadeusz, Biały, Michał, Chęcmanowski, Jacek, Zatoński, Tomasz, Bożemska, Edyta, and Wawrzyńska, Magdalena
- Subjects
STEEL alloys ,COMPOSITE materials ,STAINLESS steel ,NANOINDENTATION tests ,METALLIC glasses ,BIOMEDICAL materials ,ELASTIC deformation - Abstract
Microstructure, mechanical properties, corrosion resistance, and biocompatibility were studied for rapidly cooled 3 mm rods of Zr
40 Ti15 Cu10 Ni10 Be25 , Zr50 Ti5 Cu10 Ni10 Be25 , and Zr40 Ti15 Cu10 Ni5 Si5 Be25 (at.%) alloys, as well as for the reference 316L stainless steel and Ti-based Ti6Al4V alloy. Microstructure investigations confirm that Zr-based bulk metallic samples exhibit a glassy structure with minor fractions of crystalline phases. The nanoindentation tests carried out for all investigated composite materials allowed us to determine the mechanical parameters of individual phases observed in the samples. The instrumental hardness and elastic to total deformation energy ratio for every single phase observed in the manufactured Zr-based materials are higher than for the reference materials (316L stainless steel and Ti6Al4V alloy). A scratch tester used to determine the wear behavior of manufactured samples and reference materials revealed the effect of microstructure on mechanical parameters such as residual depth, friction force, and coefficient of friction. Electrochemical investigations in simulated body fluid performed up to 120 h show better or comparable corrosion resistance of Zr-based bulk metallic glasses in comparison with 316L stainless steel and Ti6Al4V alloy. The fibroblasts viability studies confirm the good biocompatibility of the produced materials. All obtained results show that fabricated biocompatible Zr-based materials are promising candidates for biomedical implants that require enhanced mechanical properties. [ABSTRACT FROM AUTHOR]- Published
- 2022
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39. Graphene Oxide Carboxymethylcellulose Nanocomposite for Dressing Materials
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Dariusz Hreniak, Marta Markowska, Rosa Alduina, Maria Luisa Saladino, Alessandro Presentato, Magdalena Wawrzyńska, Dariusz Biały, Roberto Scaffaro, Mariusz Hasiak, Patrizia Cancemi, Clara Carmone, Saladino, Maria Luisa, Markowska, Marta, Carmone, Clara, Cancemi, Patrizia, Alduina, Rosa, Presentato, Alessandro, Scaffaro, Roberto, Biały, Dariusz, Hasiak, Mariusz, Hreniak, Dariusz, and Wawrzyńska, Magdalena
- Subjects
Materials science ,Composite number ,Oxide ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,lcsh:Technology ,Article ,law.invention ,medical devices ,chemistry.chemical_compound ,biocompatibility ,law ,General Materials Science ,Graphite ,lcsh:Microscopy ,graphene oxide nanocomposite ,Settore CHIM/02 - Chimica Fisica ,lcsh:QC120-168.85 ,carboxymethyl cellulose ,Nanocomposite ,lcsh:QH201-278.5 ,Graphene ,lcsh:T ,021001 nanoscience & nanotechnology ,Casting ,Exfoliation joint ,0104 chemical sciences ,Solvent ,Settore ING-IND/22 - Scienza E Tecnologia Dei Materiali ,chemistry ,Chemical engineering ,lcsh:TA1-2040 ,lcsh:Descriptive and experimental mechanics ,lcsh:Electrical engineering. Electronics. Nuclear engineering ,0210 nano-technology ,lcsh:Engineering (General). Civil engineering (General) ,lcsh:TK1-9971 - Abstract
Sore, infected wounds are a major clinical issue, and there is thus an urgent need for novel biomaterials as multifunctional constituents for dressings. A set of biocomposites was prepared by solvent casting using different concentrations of carboxymethylcellulose (CMC) and exfoliated graphene oxide (Exf-GO) as a filler. Exf-GO was first obtained by the strong oxidation and exfoliation of graphite. The structural, morphological and mechanical properties of the composites (CMCx/Exf-GO) were evaluated, and the obtained composites were homogenous, transparent and brownish in color. The results confirmed that Exf-GO may be homogeneously dispersed in CMC. It was found that the composite has an inhibitory activity against the Gram-positive Staphylococcus aureus, but not against Gram-negative Pseudomonas aeruginosa. At the same time, it does not exhibit any cytotoxic effect on normal fibroblasts.
- Published
- 2020
40. Subthreshold nitric oxide synthase inhibition improves synergistic effects of subthreshold MMP-2/MLCK-mediated cardiomyocyte protection from hypoxic injury.
- Author
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Bil-Lula I, Lin HB, Biały D, Wawrzyńska M, Diebel L, Sawicka J, Woźniak M, and Sawicki G
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- Animals, Azepines, Cell Hypoxia drug effects, Doxycycline pharmacology, Drug Synergism, Imines pharmacology, Immunoblotting, Male, Myocardial Contraction drug effects, NG-Nitroarginine Methyl Ester pharmacology, Naphthalenes, Nitric Oxide metabolism, Nitric Oxide Synthase metabolism, Oxygen, Rats, Sprague-Dawley, Cardiotonic Agents pharmacology, Enzyme Inhibitors pharmacology, Matrix Metalloproteinase 2 metabolism, Myocytes, Cardiac enzymology, Myocytes, Cardiac pathology, Myosin-Light-Chain Kinase metabolism, Nitric Oxide Synthase antagonists & inhibitors
- Abstract
Injury of myocardium during ischaemia/reperfusion (I/R) is a complex and multifactorial process involving uncontrolled protein phosphorylation, nitration/nitrosylation by increased production of nitric oxide and accelerated contractile protein degradation by matrix metalloproteinase-2 (MMP-2). It has been shown that simultaneous inhibition of MMP-2 with doxycycline (Doxy) and myosin light chain kinase (MLCK) with ML-7 at subthreshold concentrations protects the heart from contractile dysfunction triggered by I/R in a synergistic manner. In this study, we showed that additional co-administration of nitric oxide synthase (NOS) inhibitor (1400W or L-NAME) in subthreshold concentrations improves this synergistic protection in the model of hypoxia-reoxygenation (H-R)-induced contractile dysfunction of cardiomyocytes. Isolated cardiomyocytes were subjected to 3 min. of hypoxia and 20 min. of reoxygenation in the presence or absence of the inhibitor cocktails. Contractility of cardiomyocytes was expressed as myocyte peak shortening. Inhibition of MMP-2 by Doxy (25-100 μM), MLCK by ML-7 (0.5-5 μM) and NOS by L-NAME (25-100 μM) or 1400W (25-100 μM) protected myocyte contractility after H-R in a concentration-dependent manner. Inhibition of these activities resulted in full recovery of cardiomyocyte contractility after H-R at the level of highest single-drug concentration. The combination of subthreshold concentrations of NOS, MMP-2 and MLCK inhibitors fully protected cardiomyocyte contractility and MLC1 from degradation by MMP-2. The observed protection with addition of L-NAME or 1400W was better than previously reported combination of ML-7 and Doxy. The results of this study suggest that addition of NOS inhibitor to the mixture of inhibitors is better strategy for protecting cardiomyocyte contractility., (© 2016 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.)
- Published
- 2016
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41. Generation of cloned and chimeric embryos/offspring using the new methods of animal biotechnology.
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Skrzyszowska M, Karasiewicz J, Bednarczyk M, Samiec M, Smorag Z, Waś B, Guszkiewicz A, Korwin-Kossakowski M, Górniewska M, Szablisty E, Modliński JA, Łakota P, Wawrzyńska M, Sechman A, Wojtysiak D, Hrabia A, Mika M, Lisowski M, Czekalski P, Rzasa J, and Kapkowska E
- Subjects
- Animals, Blastocyst physiology, Cells, Cultured, Chimera physiology, Cloning, Organism methods
- Abstract
The article summarizes results of studies concerning: 1/ qualitative evaluation of pig nuclear donor cells to somatic cell cloning, 2/ developmental potency of sheep somatic cells to create chimera, 3/ efficient production of chicken chimera. The quality of nuclear donor cells is one of the most important factors to determine the efficiency of somatic cell cloning. Morphological criteria commonly used for qualitative evaluation of somatic cells may be insufficient for practical application in the cloning. Therefore, different types of somatic cells being the source of genomic DNA in the cloning procedure were analyzed on apoptosis with the use of live-DNA or plasma membrane fluorescent markers. It has been found that morphological criteria are a sufficient selection factor for qualitative evaluation of nuclear donor cells to somatic cell cloning. Developmental potencies of sheep somatic cells in embryos and chimeric animals were studied using blastocyst complementation test. Fetal fibroblasts stained with vital fluorescent dye and microsurgically placed in morulae or blastocysts were later identified in embryos cultured in vitro. Transfer of Polish merino blastocysts harbouring Heatherhead fibroblasts to recipient ewes brought about normal births at term. Newly-born animals were of merino appearance with dark patches on their noses, near the mouth and on their clovens. This overt chimerism shows that fetal fibroblasts introduced to sheep morulae/blastocysts revealed full developmental plasticity. To achieve the efficient production of chicken chimeras, the blastodermal cells from embryos of the donor breeds, (Green-legged Partridgelike breed or GPxAraucana) were transferred into the embryos of the recipient breed (White Leghorn), and the effect of chimerism on the selected reproductive and physiological traits of recipients was examined. Using the model which allowed identification of the chimerism at many loci, it has been found that 93.9% of the examined birds were chimeras. The effect of donor cells on the reproduction and physiology of the recipients was evident.
- Published
- 2006
42. In vitro photodynamic diagnosis of atherosclerotic wall changes with the use of mono-l-aspartyl chlorin e6. A preliminary report.
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Biały D, Derkacz A, Wawrzyńska M, Bednarkiewicz A, Ziółkowski P, Nowosad H, and Strek W
- Subjects
- Aged, Aged, 80 and over, Cell Wall metabolism, Cell Wall pathology, Coronary Artery Disease pathology, Female, Humans, In Vitro Techniques, Male, Middle Aged, Spectrometry, Fluorescence instrumentation, Coronary Artery Disease diagnosis, Porphyrins pharmacokinetics, Radiation-Sensitizing Agents pharmacokinetics
- Abstract
Background: Although several methods for atherosclerosis detection are available, none of them seems to be accurate enough to identify the vulnerable atheromatous plaque. Photodynamic diagnosis (PDD) and therapy (PDT) - a new method evaluated for neoplasm treatment, is a modern approach for detecting and treating atherosclerosis., Aim: To asses in vitro the capability of PDD with the use of chlorin e6 to detect atherosclerotic plaque and the usefulness of this method as a feedback system for photoangioplasty treatment., Methods: 30 specimens of human aorta and 15 specimens of human coronary arteries were examined. The samples were soaked with chlorin e6 and then washed out. The luminescence spectra were then collected. All samples were examined with light microscopy., Results: Tissue fluorescence is seen as green light. We noted a very strong red fluorescence of chlorin e6 originating from lipid-rich plaque. We established a quantitative factor (R) which is the ratio of chlorin e6 red intensity in its 660 nm maximum to the area of green luminescence centred at 515 nm. The highest value of R was reached at the atheromatous samples, followed by calcified and normal ones R(2)=3.51+/-0.62, R(3)=1.63+/-0.31, and R(1)=1.51+/-0.15, respectively. A statistically significant difference was noted between groups two and one, and between groups two and three (R(2)=3.51+/-0.62 vs. R(3)=1.63+/-0.31, p<0.05; and R(2)=3.51+/-0.62 vs. R(1)=1.51+/-0.15, p<0.05, respectively)., Conclusions: This in vitro study confirms that photosensitiser chlorin e6 accumulates within atheromatous plaque. It may be a specific tool for atheromatous and normal or calcified segments discrimination. The advantage of the above method is the possibility of a real-time imaging followed by targeted therapy of various forms and stages of atherosclerosis.
- Published
- 2003
43. [Is it possible to detect unstable atheromatous plaque? New experimental methods tested in catheterisation laboratory].
- Author
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Derkacz A, Biały D, and Wawrzyńska M
- Subjects
- Cardiac Catheterization instrumentation, Hemodynamics physiology, Humans, Coronary Artery Disease diagnosis, Coronary Artery Disease pathology
- Published
- 2003
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