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Thirty cases of small intestine with suggestive histopathological lesions of canine parvovirus were evaluated by avidin-biotinperoxidase complex using a monoclonal antibody developed in mouse against canine parvovirus-2 (CPV-2). Positive immunohistochemical reactions were obtained in 76.67% (23 cases) of processed samples. The lymphocytes, macrophages and necrotic cells of the intestinal crypts were the cells that most frequently showed immunopositivity. The intestinal histopathological lesions which were associated to CPV-2 were the regeneration cells in the intestinal crypts by the evidence of parvoviral antigen. The immunohistochemical demonstration of parvoviral antigen is a useful and reliable method for confirming an etiologic diagnosis in cases with suggestive intestinal lesions of this disease. [ABSTRACT FROM AUTHOR]

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OBJECTIVE To evaluate the effects of a chemical restraint with isoflurane compared to a physical restraint in hemogram values in psittacines. ANIMALS 21 adult lilac-crowned parrots (Amazona finschi). METHODS Subjects were physically restrained for blood sample collection, after this isoflurane anesthesia was administered for 30 minutes at which time a second blood sample was taken. Hemogram values were compared between both samples using a t test in variables with normal distribution and a Wilcoxon test in variables without normal distribution. Statistical significance was established at P < .05. RESULTS When comparing the analytes of the respective CBCs, a decrease in PCV (P < .001), RBC count (P < .001), WBC count (P = .017), absolute lymphocytes (P = .024), and total proteins (P < .001) was observed in the samples taken under chemical restraint. No significant differences in MCV and absolute values of heterophils, monocytes, or eosinophils were observed. CLINICAL RELEVANCE Isoflurane causes a decrease in some blood count values in lilac-crowned parrots under isoflurane anesthesia for 30 minutes. Although the magnitude of change could be variable between values, it is important to consider the blood collection method when interpreting the blood count in this species. [ABSTRACT FROM AUTHOR]